FastQCFastQC Report
Fri 27 May 2016
SRR938982_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938982_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3230162
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT155120.4802235925009334No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT69470.21506661275812172No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64870.20082584093305536No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT55070.1704868053057401No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53250.16485241297495296No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC52670.16305683739700982No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT42980.13305834196551133No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC36650.11346180160623522No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC35670.1104278980435037No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT34520.10686770508723711No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG34090.1055365025035896No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32790.10151193655302737No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG86150.021.842821
TACATGG84900.021.6863652
TTTGCGT6600.020.1315333
ACATGGG85850.020.0092583
GAGTACT73050.019.16363712-13
AGAGTAC112350.018.79589810-11
TATACAG20150.018.3688645
GTACTTT77500.017.91019814-15
CATGGGG59350.017.6699094
AGTACTT79250.017.63446212-13
TCTATAC10450.017.25563
GAGTACA69850.015.9593921
CTTTGCG9050.015.7302422
GTACTAG5153.6379788E-1215.7256491
TACTTTT90100.015.2738814-15
ACCGTCC4059.222276E-915.2317948
GTAAGCG3600.015.15879894-95
GTGTACG3152.3662324E-615.123641
ACTTTTT93600.014.753098516-17
CGTATAG1950.00437767114.6582971
Produced by FastQC (version 0.11.5)