##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938982_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3230162 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13705164013446 33.0 31.0 34.0 30.0 34.0 2 32.36829360261188 34.0 31.0 34.0 30.0 34.0 3 32.47735562488816 34.0 31.0 34.0 30.0 34.0 4 36.04621935370424 37.0 35.0 37.0 35.0 37.0 5 35.95431034109125 37.0 35.0 37.0 35.0 37.0 6 35.99309910772277 37.0 35.0 37.0 35.0 37.0 7 35.93193251607814 37.0 35.0 37.0 35.0 37.0 8 35.892789278060974 37.0 35.0 37.0 35.0 37.0 9 37.57503988964021 39.0 37.0 39.0 35.0 39.0 10-11 37.490024648918535 39.0 37.0 39.0 34.5 39.0 12-13 37.41797981030054 39.0 37.0 39.0 34.0 39.0 14-15 38.679213147823546 40.0 38.0 41.0 34.0 41.0 16-17 38.671072534442544 40.0 38.0 41.0 34.0 41.0 18-19 38.75283468754817 40.0 38.0 41.0 34.0 41.0 20-21 38.74049799979072 40.0 38.0 41.0 34.0 41.0 22-23 38.682064243217525 40.0 38.0 41.0 34.0 41.0 24-25 38.62026486597267 40.0 38.0 41.0 34.0 41.0 26-27 38.46395892837573 40.0 38.0 41.0 34.0 41.0 28-29 38.29008730831457 40.0 38.0 41.0 34.0 41.0 30-31 38.15258615512163 40.0 38.0 41.0 33.0 41.0 32-33 38.02406860708534 40.0 37.5 41.0 33.0 41.0 34-35 38.012691468725095 40.0 37.0 41.0 33.0 41.0 36-37 37.928858366855906 40.0 37.0 41.0 33.0 41.0 38-39 37.81266899307217 40.0 37.0 41.0 33.0 41.0 40-41 37.67879985585862 40.0 37.0 41.0 33.0 41.0 42-43 37.527775696698804 40.0 37.0 41.0 32.0 41.0 44-45 37.30878544172088 40.0 37.0 41.0 31.5 41.0 46-47 37.06297872985937 40.0 36.0 41.0 31.0 41.0 48-49 36.81353675140751 39.5 36.0 41.0 30.5 41.0 50-51 36.54984285617873 39.0 35.5 41.0 30.0 41.0 52-53 36.329275900094174 39.0 35.0 40.5 30.0 41.0 54-55 36.08009102949016 39.0 35.0 40.0 29.0 41.0 56-57 35.7750245962896 38.0 35.0 40.0 28.5 41.0 58-59 35.45541539402667 38.0 34.5 40.0 28.0 41.0 60-61 35.01895199064319 38.0 34.0 40.0 27.5 41.0 62-63 34.69519051985628 37.0 34.0 40.0 27.0 41.0 64-65 34.42269876866857 37.0 34.0 39.5 26.5 41.0 66-67 33.94663704173351 36.0 33.0 39.0 26.0 40.5 68-69 33.50759667781368 35.5 33.0 39.0 26.0 40.0 70-71 33.03041720508135 35.0 32.0 38.0 25.0 40.0 72-73 32.503124146714626 35.0 32.0 37.0 24.0 39.5 74-75 32.03337789250199 35.0 31.0 37.0 22.5 39.0 76-77 30.07019864638368 33.0 29.0 35.0 20.0 37.0 78-79 31.064419369678674 34.0 30.5 36.0 21.0 37.5 80-81 31.06327422587474 34.0 31.0 35.0 20.5 37.0 82-83 30.740328348856806 34.0 31.0 35.0 20.0 37.0 84-85 30.33616255159958 34.0 30.0 35.0 18.5 36.0 86-87 29.765751686757504 34.0 29.5 35.0 13.5 36.0 88-89 29.181185030348324 34.0 29.0 35.0 7.0 35.5 90-91 28.697406043412066 33.5 29.0 35.0 2.0 35.0 92-93 28.31577998255196 33.0 29.0 35.0 2.0 35.0 94-95 27.88794818959544 33.0 27.5 35.0 2.0 35.0 96-97 27.21564073257007 33.0 26.5 35.0 2.0 35.0 98-99 26.261647248651926 33.0 25.0 34.0 2.0 35.0 100-101 21.831412170658936 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15196571027500028 1101 2 0.1750375066994181 1101 3 0.18339940351063433 1101 4 0.07907584944221924 1101 5 0.13391307227450255 1101 6 0.17871863492263884 1101 7 0.12851004313352377 1101 8 0.14759078462866881 1101 9 0.28088218889627115 1101 10-11 0.31363018035721524 1101 12-13 0.2865052198202278 1101 14-15 0.2773221244660604 1101 16-17 0.29147697714831367 1101 18-19 0.3445169324073234 1101 20-21 0.29704440793571507 1101 22-23 0.30030039962946375 1101 24-25 0.32608366287782786 1101 26-27 0.2743193408300826 1101 28-29 0.31181264486496474 1101 30-31 0.3232557743265403 1101 32-33 0.36489703523285044 1101 34-35 0.3853477286757183 1101 36-37 0.3324393001640473 1101 38-39 0.469033988258019 1101 40-41 0.41318666215953925 1101 42-43 0.3800657456660943 1101 44-45 0.5138421616188182 1101 46-47 0.5301972222270663 1101 48-49 0.31226400152478107 1101 50-51 0.43466389441123 1101 52-53 0.385236230634284 1101 54-55 0.39258076484574644 1101 56-57 0.1771708558629399 1101 58-59 0.26047302746548695 1101 60-61 0.3999784877900652 1101 62-63 0.34287858463907384 1101 64-65 0.2751587230836847 1101 66-67 0.393776502202563 1101 68-69 0.2887512203793676 1101 70-71 0.2905391958587913 1101 72-73 0.24262871405340292 1101 74-75 0.30526757292539486 1101 76-77 0.09647373813491811 1101 78-79 0.4251779550777819 1101 80-81 0.6537043443850656 1101 82-83 0.606049063458503 1101 84-85 0.3929032000014061 1101 86-87 0.52236042005568 1101 88-89 0.8006424031554644 1101 90-91 0.7723370179415916 1101 92-93 0.7143467628250306 1101 94-95 0.5905272369598933 1101 96-97 0.477189243116122 1101 98-99 0.45857582486416604 1101 100-101 1.2605376452955 1102 1 -0.02468214524346024 1102 2 0.00719158713649648 1102 3 0.014088427881411292 1102 4 0.026142065889963817 1102 5 0.021821437896818452 1102 6 0.038980146419504536 1102 7 0.028124703697478992 1102 8 0.08135731000776047 1102 9 0.06175526313024449 1102 10-11 0.08197908441184154 1102 12-13 0.053786379265190476 1102 14-15 0.09266067724563953 1102 16-17 0.1342914235993291 1102 18-19 0.07918205104738973 1102 20-21 0.11060971823452803 1102 22-23 0.18148315012935967 1102 24-25 0.12706584744702099 1102 26-27 0.030642536433745704 1102 28-29 0.031247118537699237 1102 30-31 0.015459381682568107 1102 32-33 0.08379372574068356 1102 34-35 0.07822118388599364 1102 36-37 -0.03170848884319355 1102 38-39 -0.009006133441559427 1102 40-41 -0.05076861675660638 1102 42-43 -0.06653600959175066 1102 44-45 -0.0830913516318077 1102 46-47 0.08060017383562723 1102 48-49 -0.07029393507352921 1102 50-51 -0.10581717862235962 1102 52-53 -0.04791652915590561 1102 54-55 0.0044265205250084705 1102 56-57 -0.06277067810940196 1102 58-59 -0.03629181331728404 1102 60-61 -0.16398392112947136 1102 62-63 -0.12333206332925073 1102 64-65 -0.0814348805583549 1102 66-67 -0.05126367589853231 1102 68-69 -0.022761127828715644 1102 70-71 -0.13552208385975462 1102 72-73 -0.10890237435757655 1102 74-75 -0.04833371728596347 1102 76-77 -0.3107186793725383 1102 78-79 -0.32424627054531996 1102 80-81 -0.1184230889946285 1102 82-83 -0.05314949223693333 1102 84-85 -0.08831450879861436 1102 86-87 -0.07964348335512028 1102 88-89 0.05493365421565599 1102 90-91 0.1270167477676729 1102 92-93 0.02429497984530471 1102 94-95 -0.12337775946978269 1102 96-97 -0.27567457062690437 1102 98-99 -0.01668082865278464 1102 100-101 0.39719975017891684 1103 1 0.11105479745866376 1103 2 0.07579527382301876 1103 3 0.06855770819408491 1103 4 0.10568811079522789 1103 5 0.0689782864483135 1103 6 0.07133286162560637 1103 7 0.07343366190983858 1103 8 0.09783706741335152 1103 9 0.03710341505698267 1103 10-11 0.1392249041061291 1103 12-13 0.10627329073882663 1103 14-15 0.11500532792296525 1103 16-17 0.1193781604024764 1103 18-19 0.18878391001796047 1103 20-21 0.1611097137919799 1103 22-23 0.14097365797515948 1103 24-25 0.18263243677852614 1103 26-27 0.05086072296530375 1103 28-29 0.11683868543435239 1103 30-31 0.1199768244272903 1103 32-33 0.14137357267666317 1103 34-35 0.06076098257068452 1103 36-37 -0.0027319522839093224 1103 38-39 0.1671714158092925 1103 40-41 0.19678325258860951 1103 42-43 0.2657010426060822 1103 44-45 0.31113506249611333 1103 46-47 0.2904823313662419 1103 48-49 0.29193955786047354 1103 50-51 0.159094176892701 1103 52-53 0.31813467929517714 1103 54-55 0.2622252020816944 1103 56-57 0.22531843624017966 1103 58-59 0.22034887926005808 1103 60-61 0.23947342545763917 1103 62-63 0.27590439356733754 1103 64-65 0.31639835878462463 1103 66-67 0.28627030305006684 1103 68-69 0.41769343354297206 1103 70-71 0.1960870535701673 1103 72-73 0.39829779163687107 1103 74-75 0.3216990059898919 1103 76-77 0.20648892396762974 1103 78-79 0.3059686389547771 1103 80-81 0.344153891192132 1103 82-83 0.37714428553224977 1103 84-85 0.34972759295451894 1103 86-87 0.15638656289440078 1103 88-89 0.37128849766292404 1103 90-91 0.4441697861821119 1103 92-93 0.40922983381879874 1103 94-95 0.20240735611744043 1103 96-97 0.17805771733164732 1103 98-99 0.37904256689536453 1103 100-101 0.7915255205556981 1104 1 0.010766955678334966 1104 2 -0.049006499580436014 1104 3 -0.0014007248082563706 1104 4 0.02877302572917273 1104 5 0.0679498278606161 1104 6 0.029991711771749863 1104 7 -0.09878494091219636 1104 8 -0.004209917025463028 1104 9 0.018188350126251862 1104 10-11 -0.01893368692740438 1104 12-13 0.085765822348435 1104 14-15 0.1552692377241769 1104 16-17 0.18024787995160807 1104 18-19 0.0493352941816525 1104 20-21 0.0056493605124572355 1104 22-23 -0.016590525718399363 1104 24-25 0.020931792437650643 1104 26-27 0.10035055706368468 1104 28-29 0.06418137740524088 1104 30-31 0.03606585454486577 1104 32-33 0.0219417044513861 1104 34-35 0.10983991066960641 1104 36-37 0.19435035564400494 1104 38-39 0.31458142620181917 1104 40-41 0.15804381546237067 1104 42-43 0.13087862218811352 1104 44-45 0.1418827879046276 1104 46-47 0.18000435292827177 1104 48-49 0.11644756305454962 1104 50-51 0.06546505697363614 1104 52-53 -0.004048940989065386 1104 54-55 0.12571598087985336 1104 56-57 0.11972078389488416 1104 58-59 0.1414226485633776 1104 60-61 0.23685827187957642 1104 62-63 0.27865758831217846 1104 64-65 0.2640748405242235 1104 66-67 0.3692835382918318 1104 68-69 0.3682247726761787 1104 70-71 0.15794908152775378 1104 72-73 0.09348758630530796 1104 74-75 0.21911423184756273 1104 76-77 0.08777538876136859 1104 78-79 0.29877275013209825 1104 80-81 0.30935947341085424 1104 82-83 0.5012337976522119 1104 84-85 0.3695650427342585 1104 86-87 0.14326933075887638 1104 88-89 0.28774183191238123 1104 90-91 0.4222334879251761 1104 92-93 0.5037974260912712 1104 94-95 0.5846949826365808 1104 96-97 0.3837139904671645 1104 98-99 0.4102591755933922 1104 100-101 0.9680818854107507 1105 1 -0.06810918445892611 1105 2 -0.015952392004500382 1105 3 -0.008388239857524127 1105 4 0.0030838002418605015 1105 5 0.015165281581722923 1105 6 0.01015718589190584 1105 7 0.029129627598493357 1105 8 0.07832856104449348 1105 9 -0.02259657739743659 1105 10-11 0.046504900719810394 1105 12-13 0.014512282108839258 1105 14-15 0.07211261448362905 1105 16-17 0.08384009170003281 1105 18-19 0.08496637575559163 1105 20-21 0.1429681034087693 1105 22-23 0.08830235735150183 1105 24-25 0.022576063693726667 1105 26-27 0.028483871919853243 1105 28-29 0.11017352106840406 1105 30-31 0.16305950665572055 1105 32-33 0.12948574914437216 1105 34-35 0.17996813586691474 1105 36-37 0.0565441378026037 1105 38-39 0.14387580290229351 1105 40-41 0.20363899920582895 1105 42-43 0.11280827191027498 1105 44-45 0.12164334249742836 1105 46-47 0.2253523806497526 1105 48-49 0.26272045636581254 1105 50-51 0.2234832697804876 1105 52-53 0.3915177412362496 1105 54-55 0.343597535387147 1105 56-57 0.4114909679802068 1105 58-59 0.3526221124531901 1105 60-61 0.2961136730346112 1105 62-63 0.2292901117996493 1105 64-65 0.259689096712151 1105 66-67 0.38029759434665067 1105 68-69 0.25097107814265485 1105 70-71 0.20743676852495696 1105 72-73 0.14655918869066653 1105 74-75 0.33495004473473244 1105 76-77 0.22875289797155673 1105 78-79 0.4008241553191887 1105 80-81 0.2902471288553592 1105 82-83 0.38224067373275616 1105 84-85 0.27551773735738294 1105 86-87 0.28421219526655506 1105 88-89 0.40581011065130923 1105 90-91 0.5519021804820632 1105 92-93 0.48078130217120574 1105 94-95 0.500609942596693 1105 96-97 0.3379142039887455 1105 98-99 0.37573830621647275 1105 100-101 0.8350265328705468 1106 1 -0.04564928682981417 1106 2 -0.14748711266202008 1106 3 -0.1086121425619524 1106 4 -0.0863719940648906 1106 5 -0.09083030650504043 1106 6 -0.06976307055926867 1106 7 -0.11322579538317967 1106 8 -0.09971861451224129 1106 9 -0.20873734065882132 1106 10-11 -0.14455857437208408 1106 12-13 -0.0622663339178402 1106 14-15 -0.09060796374674851 1106 16-17 -0.08818169254494279 1106 18-19 -0.18077738703305357 1106 20-21 -0.21695375078214596 1106 22-23 -0.06937597739729284 1106 24-25 -0.14678006512234276 1106 26-27 2.1264424412947847E-4 1106 28-29 -0.00808083471476806 1106 30-31 -0.10414787139559678 1106 32-33 -0.12761453053976624 1106 34-35 -0.08045616961933177 1106 36-37 -0.04543548563683686 1106 38-39 -0.011735381355549634 1106 40-41 0.05389911003955916 1106 42-43 0.07833863085784998 1106 44-45 -0.1063473443931926 1106 46-47 -0.04043799948281901 1106 48-49 -0.061751796453194174 1106 50-51 -0.059947890248288616 1106 52-53 0.037738319765459494 1106 54-55 0.012879703994109093 1106 56-57 0.01615850961855614 1106 58-59 -0.009850696586433116 1106 60-61 -0.045230372725285406 1106 62-63 -0.023108988047326307 1106 64-65 0.0013040011934108975 1106 66-67 0.006796745373101487 1106 68-69 -0.055252875163503745 1106 70-71 -0.16141444863596632 1106 72-73 -0.058122527409317115 1106 74-75 0.1085388191986354 1106 76-77 -0.044119632878068415 1106 78-79 -0.057594319413116324 1106 80-81 0.22520358247877326 1106 82-83 0.3535701605375543 1106 84-85 0.18835291655823738 1106 86-87 0.13544564237410128 1106 88-89 0.23590687550725065 1106 90-91 0.5232462103044178 1106 92-93 0.487791136239359 1106 94-95 0.40755699266511414 1106 96-97 0.3553993935191251 1106 98-99 0.45623286244625305 1106 100-101 0.8600652262620976 1107 1 -0.03217850060210026 1107 2 -0.020991060298406694 1107 3 -0.029586694081721987 1107 4 -0.1020662365623295 1107 5 -0.15643104819653075 1107 6 -0.1071165241293528 1107 7 -0.12196549608587759 1107 8 -0.012976290735018381 1107 9 -0.008794609409108034 1107 10-11 0.07140687571845916 1107 12-13 -0.06677876411785633 1107 14-15 0.02414986956053866 1107 16-17 0.0055352032756630365 1107 18-19 -0.08014660194202605 1107 20-21 -0.06809732997246698 1107 22-23 -0.09736958192637957 1107 24-25 -0.1518013919102259 1107 26-27 -0.14943827687103095 1107 28-29 -0.10255616652619892 1107 30-31 -0.11437434937140267 1107 32-33 -0.04105992867401653 1107 34-35 -0.19287579207973238 1107 36-37 -0.15422992910772138 1107 38-39 -0.17169788433541555 1107 40-41 -0.18942721566688903 1107 42-43 -0.1839503707003658 1107 44-45 -0.22026913683009042 1107 46-47 -0.3466707024257403 1107 48-49 -0.2907944827877884 1107 50-51 -0.1749024245440154 1107 52-53 -0.09131015856713987 1107 54-55 -0.16444640976664715 1107 56-57 -0.13798915297969927 1107 58-59 -0.15761255804579122 1107 60-61 -0.2006908553141642 1107 62-63 -0.19292430126120763 1107 64-65 -0.07766786883849619 1107 66-67 0.054540619077151575 1107 68-69 -0.020069743435115583 1107 70-71 -0.06998593471858783 1107 72-73 -0.050888795523370334 1107 74-75 -0.07696955245858916 1107 76-77 -0.3580507102725825 1107 78-79 -0.12183093210270002 1107 80-81 0.033050496518018946 1107 82-83 0.08955150260763034 1107 84-85 0.1231625482858334 1107 86-87 0.13342357397365845 1107 88-89 0.19493288049291024 1107 90-91 0.41983171400892516 1107 92-93 0.5498645314118882 1107 94-95 0.41389266884683806 1107 96-97 0.37060092150148094 1107 98-99 0.37716705745522816 1107 100-101 0.9843663876613 1108 1 0.019064246618306413 1108 2 0.00999084697330943 1108 3 0.012783337083483559 1108 4 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-2.074366788343415 2316 94-95 -2.3060254997391993 2316 96-97 -2.4674544914185503 2316 98-99 -2.868073380302988 2316 100-101 -3.52793339453099 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 9.0 4 15.0 5 61.0 6 131.0 7 318.0 8 812.0 9 1511.0 10 2532.0 11 3746.0 12 5377.0 13 7434.0 14 9161.0 15 12163.0 16 15194.0 17 20456.0 18 20728.0 19 16626.0 20 16537.0 21 17972.0 22 19655.0 23 22169.0 24 25872.0 25 31072.0 26 37923.0 27 45795.0 28 56189.0 29 68485.0 30 82797.0 31 101414.0 32 125379.0 33 159978.0 34 214726.0 35 307029.0 36 486644.0 37 736135.0 38 521140.0 39 36972.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.63651116766148 19.267087344208036 14.299430776724167 23.796970711406317 2 15.832363825715243 20.600793396739853 38.93377483853751 24.633067939007393 3 20.279168660890694 23.474797858435583 29.351871516041612 26.89416196463211 4 13.528671317413801 16.305405115904403 36.18149801774648 33.98442554893531 5 14.109610287809895 36.93385421681264 34.29113110122029 14.66540439415718 6 33.19461996023729 35.578432289154534 16.575794031382944 14.651153719225226 7 29.07157597668476 31.76583713138846 20.57150693989961 18.591079952027172 8 25.967892632010408 35.36844282113405 19.27141734686991 19.392247199985636 9 25.608746558222155 17.144527116596628 20.355511581152896 36.89121474402832 10-11 25.492962891644446 26.0115436934742 26.91835579763492 21.577137617246443 12-13 26.236718158408156 23.21710180480112 27.398842534832617 23.147337501958106 14-15 22.864488885483443 24.86866984146886 25.677245986800813 26.589595286246887 16-17 22.625613489667614 27.898598396885284 26.405062773850986 23.070725339596116 18-19 22.598974912094192 27.350331036028535 27.743175110102836 22.307518941774436 20-21 23.38260433996809 26.087700864538682 27.678472472897642 22.851222322595586 22-23 22.911668207353067 26.008556227210892 27.79944163791166 23.280333927524378 24-25 23.26940568305862 26.122761025608003 27.752911463883233 22.854921827450138 26-27 22.988599333408047 26.014252535940923 28.204576117234986 22.792572013416045 28-29 22.644297716337448 27.011122042795378 27.69475648589761 22.649823754969564 30-31 22.924686130293157 26.263682750276924 27.579886086208678 23.231745033221245 32-33 22.92847850974657 26.209227277145853 27.760449785490636 23.101844427616943 34-35 23.33240456520494 26.248225665888047 27.700540388256034 22.718829380650973 36-37 23.187520511216928 25.97266512690638 27.985132850765034 22.85468151111166 38-39 22.803452020133996 26.507410300638345 27.898983856700234 22.790153822527426 40-41 23.050056693510626 26.5608748988998 27.573850553584073 22.815217854005503 42-43 22.752850034797298 26.4753403571862 27.30781304564266 23.463996562373847 44-45 23.076764713212192 26.19956135157212 27.4021372694979 23.321536665717794 46-47 23.467720114892355 26.645184718747156 27.167899734811407 22.719195431549082 48-49 23.254695135089616 26.538642763560354 27.23980171830923 22.9668603830408 50-51 22.687855634740288 26.791996631724942 27.608865250422582 22.911282483112185 52-53 23.183899135708984 26.96788891702645 26.747760638630506 23.100451308634057 54-55 23.016539108564835 26.55447311930485 26.9733066019599 23.45568117017041 56-57 23.139814659450515 26.61522858605853 27.01047192060336 23.23448483388759 58-59 23.543566990036382 26.485564765992436 26.945821744611397 23.02504649935978 60-61 23.08853147522119 26.706234209991457 27.46856626374169 22.736668051045662 62-63 22.824133897928338 26.672516734454803 27.474349583705088 23.028999783911765 64-65 22.68441524186448 27.129974527546036 27.05973024213072 23.12587998845876 66-67 22.984738747939893 26.657866136694793 26.743405058994146 23.613990056371172 68-69 22.727666747675386 26.55461664558892 27.219393796796084 23.498322809939616 70-71 23.227565676272583 26.421151632642577 26.92731819642482 23.42396449466002 72-73 23.092030678337494 26.197633431388272 27.281557395573348 23.428778494700886 74-75 22.602983998944946 27.24241075215423 26.705301467852077 23.44930378104875 76-77 22.891302050060798 26.473259382486564 26.810467556692913 23.824971010759725 78-79 22.946319816813944 26.591636292770154 26.930288405352243 23.53175548506366 80-81 23.20550795904354 26.43855014082885 26.809878266167452 23.546063633960156 82-83 23.006477693688428 26.327843618988766 27.225693324359586 23.43998536296322 84-85 22.72717281671941 26.641187036439657 26.9833525377365 23.648287609104436 86-87 22.719916514595994 26.583999747013927 26.996171725458023 23.699912012932053 88-89 22.72351176132212 26.562475707778177 26.611168980581766 24.10284355031794 90-91 22.80001329512951 26.214485229980887 26.791969120508547 24.193532354381055 92-93 22.986772799630483 26.46641252048659 26.775979037583873 23.770835642299055 94-95 22.861902901464383 26.21294226110022 26.94030516116529 23.984849676270105 96-97 22.399849294667778 26.110441808408265 27.366082229448246 24.123626667475712 98-99 22.56502924621118 26.462635620132986 26.67420395633408 24.298131177321757 100-101 22.69039018548949 25.553471720870448 27.325847420372266 24.430290673267795 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 325.0 1 247.0 2 204.5 3 270.5 4 250.0 5 253.5 6 461.0 7 931.5 8 1507.5 9 1794.5 10 1731.0 11 1666.0 12 1747.0 13 1895.5 14 2094.0 15 2278.5 16 2468.0 17 2679.0 18 2961.5 19 3317.0 20 3871.0 21 4650.0 22 5795.0 23 7390.5 24 9223.0 25 11416.5 26 14767.5 27 19112.0 28 22725.5 29 26375.0 30 30570.5 31 36078.5 32 42058.0 33 46644.5 34 51385.0 35 56906.5 36 63620.0 37 69735.0 38 75601.5 39 80505.5 40 84827.0 41 89830.5 42 93075.5 43 96954.5 44 102553.0 45 107500.0 46 113269.0 47 128658.5 48 151905.5 49 150790.0 50 131564.0 51 124895.5 52 125098.0 53 125811.0 54 123505.0 55 115589.0 56 106720.5 57 97825.5 58 89875.0 59 81216.5 60 68199.5 61 55024.0 62 43127.5 63 32271.0 64 23670.5 65 17123.0 66 12052.0 67 9025.5 68 6818.0 69 4995.0 70 3516.0 71 2149.0 72 1346.5 73 843.0 74 496.5 75 297.5 76 178.0 77 87.0 78 48.5 79 23.5 80 14.5 81 13.0 82 8.5 83 6.5 84 5.0 85 2.0 86 1.0 87 2.0 88 2.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4015588072672516 2 0.0 3 0.0 4 0.0 5 2.786237965773853E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0012228488849785242 16-17 0.0023218649714782107 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.00238378137071763 36-37 0.006872720315575503 38-39 0.012414238047503499 40-41 0.0023063858716683558 42-43 8.049131901124463E-4 44-45 3.869774952463685E-4 46-47 2.4766559695767583E-4 48-49 3.095819961970947E-4 50-51 6.810803916336084E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.095819961970947E-4 60-61 0.0022754276720486463 62-63 0.0 64-65 1.8574919771825686E-4 66-67 0.0016562636796544571 68-69 0.0017646173783234403 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.12274926149214807 78-79 1.0835369866898317E-4 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14513203981719802 88-89 0.4368821130333401 90-91 0.33862078744038226 92-93 0.0 94-95 0.0 96-97 5.417684933449158E-4 98-99 0.0 100-101 1.8574919771825686E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3230162.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.854726515333006 #Duplication Level Percentage of deduplicated Percentage of total 1 72.78243990478515 37.01331076476509 2 14.14653547178791 14.38836385114463 3 5.215831848881871 7.957491066745538 4 2.497517362549163 5.08042249758934 5 1.4330201756504497 3.643792456182904 6 0.8911299858665711 2.7190903032514218 7 0.6079182469608705 2.1640861335013017 8 0.4143647395451417 1.6857924405732294 9 0.30848757815305367 1.4119246278315867 >10 1.5660849105375245 14.127816072734841 >50 0.09633196818989676 3.3235374215262605 >100 0.0372344193170931 3.1886480390189655 >500 0.001460417777803376 0.4626967837600312 >1k 0.0012778655535351407 1.439088496696821 >5k 3.042537032226525E-4 0.9139127940165838 >10k+ 6.085074064453051E-5 0.48002625066147186 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 15512 0.4802235925009334 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6947 0.21506661275812172 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6487 0.20082584093305536 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5507 0.1704868053057401 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5325 0.16485241297495296 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5267 0.16305683739700982 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4298 0.13305834196551133 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3665 0.11346180160623522 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3567 0.1104278980435037 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3452 0.10686770508723711 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3409 0.1055365025035896 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3279 0.10151193655302737 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.095819961970948E-5 2 3.095819961970948E-5 0.0 0.0 0.0 3.095819961970948E-5 3 3.095819961970948E-5 0.0 0.0 0.0 3.095819961970948E-5 4 3.095819961970948E-5 0.0 0.0 0.0 3.095819961970948E-5 5 3.095819961970948E-5 0.0 0.0 0.0 3.095819961970948E-5 6 3.095819961970948E-5 0.0 0.0 0.0 1.547909980985474E-4 7 3.095819961970948E-5 0.0 0.0 0.0 1.547909980985474E-4 8 3.095819961970948E-5 0.0 0.0 0.0 1.8574919771825686E-4 9 3.095819961970948E-5 0.0 0.0 0.0 1.8574919771825686E-4 10-11 3.095819961970948E-5 0.0 0.0 3.095819961970948E-5 1.8574919771825686E-4 12-13 3.095819961970948E-5 0.0 0.0 3.095819961970948E-5 1.8574919771825686E-4 14-15 3.095819961970948E-5 0.0 0.0 4.643729942956422E-5 2.4766559695767583E-4 16-17 3.095819961970948E-5 0.0 0.0 1.0835369866898318E-4 2.4766559695767583E-4 18-19 3.095819961970948E-5 0.0 0.0 1.547909980985474E-4 2.786237965773853E-4 20-21 3.095819961970948E-5 0.0 0.0 1.8574919771825686E-4 3.2506109600694954E-4 22-23 3.095819961970948E-5 0.0 0.0 3.095819961970948E-4 3.714983954365137E-4 24-25 3.095819961970948E-5 0.0 0.0 4.024565950562232E-4 4.3341479467593266E-4 26-27 3.095819961970948E-5 0.0 0.0 5.8820579277448E-4 5.262893935350611E-4 28-29 3.095819961970948E-5 0.0 0.0 0.002074199374520535 5.262893935350611E-4 30-31 3.095819961970948E-5 0.0 0.0 0.008234881098842722 5.417684933449159E-4 32-33 3.095819961970948E-5 0.0 0.0 0.020014476054142177 5.572475931547706E-4 34-35 3.095819961970948E-5 0.0 0.0 0.036824778447644424 5.572475931547706E-4 36-37 3.095819961970948E-5 0.0 0.0 0.06510509380024902 5.572475931547706E-4 38-39 3.095819961970948E-5 0.0 0.0 0.10935984015662373 5.727266929646253E-4 40-41 3.095819961970948E-5 0.0 0.0 0.15814996275728588 5.8820579277448E-4 42-43 6.191639923941896E-5 0.0 0.0 0.2091690757305671 6.036848925843347E-4 44-45 9.287459885912843E-5 0.0 0.0 0.27254051035211235 6.346430922040443E-4 46-47 1.7027009790840212E-4 0.0 0.0 0.3525674563690614 6.656012918237538E-4 48-49 1.8574919771825686E-4 0.0 0.0 0.43442093616357325 7.739549904927369E-4 50-51 1.8574919771825686E-4 0.0 0.0 0.5276670334181381 8.203922899223011E-4 52-53 1.8574919771825686E-4 0.0 0.0 0.6246590728266879 8.8230868916172E-4 54-55 1.8574919771825686E-4 0.0 0.0 0.7327960640983331 9.132668887814296E-4 56-57 1.8574919771825686E-4 0.0 0.0 0.8621239430096694 9.287459885912843E-4 58-59 1.8574919771825686E-4 0.0 0.0 1.004330432962805 9.287459885912843E-4 60-61 1.8574919771825686E-4 0.0 0.0 1.1573877718826486 9.597041882109938E-4 62-63 1.8574919771825686E-4 0.0 0.0 1.3154293809412656 9.597041882109938E-4 64-65 1.8574919771825686E-4 0.0 0.0 1.484600462763168 0.001006141487640558 66-67 2.1670739733796633E-4 0.0 0.0 1.6853179499975544 0.0010216205874504127 68-69 2.1670739733796633E-4 0.0 0.0 1.916157765461918 0.0010216205874504127 70-71 2.1670739733796633E-4 0.0 0.0 2.170061439642965 0.0010525787870701222 72-73 2.1670739733796633E-4 0.0 0.0 2.423376908031238 0.0011609324857391053 74-75 2.1670739733796633E-4 0.0 0.0 2.692233392628605 0.0012073697851686695 76-77 2.1670739733796633E-4 0.0 0.0 2.9813674979768816 0.0012073697851686695 78-79 2.1670739733796633E-4 0.0 0.0 3.3276968771225715 0.0012692861844080885 80-81 2.1670739733796633E-4 0.0 0.0 3.7059905973756115 0.001300244384027798 82-83 2.1670739733796633E-4 0.0 0.0 4.085027314419524 0.0013931189828869264 84-85 2.1670739733796633E-4 0.0 0.0 4.473722989744787 0.0014240771825066358 86-87 2.1670739733796633E-4 0.0 0.0 4.884956234393197 0.0014395562823164908 88-89 2.1670739733796633E-4 0.0 0.0 5.34073832829437 0.0014550353821263455 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 8615 0.0 21.84282 1 TACATGG 8490 0.0 21.686365 2 TTTGCGT 660 0.0 20.131533 3 ACATGGG 8585 0.0 20.009258 3 GAGTACT 7305 0.0 19.163637 12-13 AGAGTAC 11235 0.0 18.795898 10-11 TATACAG 2015 0.0 18.368864 5 GTACTTT 7750 0.0 17.910198 14-15 CATGGGG 5935 0.0 17.669909 4 AGTACTT 7925 0.0 17.634462 12-13 TCTATAC 1045 0.0 17.2556 3 GAGTACA 6985 0.0 15.959392 1 CTTTGCG 905 0.0 15.730242 2 GTACTAG 515 3.6379788E-12 15.725649 1 TACTTTT 9010 0.0 15.27388 14-15 ACCGTCC 405 9.222276E-9 15.231794 8 GTAAGCG 360 0.0 15.158798 94-95 GTGTACG 315 2.3662324E-6 15.12364 1 ACTTTTT 9360 0.0 14.7530985 16-17 CGTATAG 195 0.004377671 14.658297 1 >>END_MODULE