FastQCFastQC Report
Fri 27 May 2016
SRR938981_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938981_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences949384
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92310.9723146798345033No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73620.7754501866473419No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA60490.6371499835682928No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA49730.5238133358051116No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46000.4845247023333024No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT36110.38035189133164243No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33520.35307104396113687No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31750.3344273760670076No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT24120.25405947435389686No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG22830.24047171639715856No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA21220.22351335181549298No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT20480.21571882399534856No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC17480.18411938688665494No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16280.17147961204317747No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG15990.1684249997893371No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT14580.15357326434825108No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT14070.14820136003977316No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13840.14577873652810666No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG12090.12734573154803536No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA10470.1102820355093408No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT10290.10838606928281919No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG10060.10596344577115267No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9990.10522612557194982No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG459.767726E-442.0954252
ATCACGC651.3517156E-436.428733
GAGTACT65750.025.13058512-13
GTATGGT2052.6904672E-823.1524261
GTACATG64100.023.101861
GTACTTT72350.022.9689714-15
AGTACTT68500.022.25554512-13
CGCAGAA3007.2759576E-1222.0942759
TACATGG68300.021.4945532
ACTTTTT81950.020.85600716-17
ACATGGG66350.020.8415183
GGATATG3553.6379788E-1220.054571
CATGGGA43500.019.9227494
CATGGGG17700.019.799124
GTTACAC1751.016638E-418.9429423
GTAGTAC1250.006136042518.9429423
ATGGGAG14050.018.8755285
TACTTTT81550.018.78119714-15
ACGCAGT1559.83656E-418.3270477
TACCTGG5250.018.0408952
Produced by FastQC (version 0.11.5)