##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938981_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 949384 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.841940668896886 33.0 31.0 34.0 30.0 34.0 2 31.901344450717517 33.0 31.0 34.0 30.0 34.0 3 31.904962586266464 34.0 31.0 34.0 30.0 34.0 4 35.47012905210115 37.0 35.0 37.0 33.0 37.0 5 35.523257185712 37.0 35.0 37.0 33.0 37.0 6 35.62931964305276 37.0 35.0 37.0 33.0 37.0 7 35.57353821003935 37.0 35.0 37.0 33.0 37.0 8 35.57142842095506 37.0 35.0 37.0 33.0 37.0 9 37.15169309784029 39.0 37.0 39.0 33.0 39.0 10-11 37.05209535867468 39.0 37.0 39.0 33.0 39.0 12-13 36.953972259907474 39.0 37.0 39.0 33.0 39.0 14-15 38.08522631516857 40.0 38.0 41.0 33.0 41.0 16-17 38.022535138574064 40.0 38.0 41.0 33.0 41.0 18-19 38.03930548650493 40.0 38.0 41.0 33.0 41.0 20-21 38.14632804007651 40.0 38.0 41.0 33.0 41.0 22-23 38.07602350576795 40.0 38.0 41.0 33.0 41.0 24-25 37.984389351411025 40.0 38.0 41.0 33.0 41.0 26-27 37.82695516250537 40.0 37.5 41.0 33.0 41.0 28-29 37.674624282692776 40.0 37.0 41.0 32.5 41.0 30-31 37.42681412368441 40.0 37.0 41.0 32.0 41.0 32-33 37.14695897550412 40.0 36.0 41.0 31.5 41.0 34-35 36.801214787693915 39.5 35.0 41.0 30.5 41.0 36-37 36.48948792058851 39.0 35.0 41.0 30.0 41.0 38-39 36.19594810951101 39.0 35.0 41.0 30.0 41.0 40-41 36.00082316533668 39.0 35.0 41.0 30.0 41.0 42-43 35.72900059406942 39.0 35.0 41.0 28.5 41.0 44-45 35.48509454551583 39.0 35.0 41.0 26.5 41.0 46-47 35.077041534300136 39.0 35.0 41.0 24.0 41.0 48-49 34.71180260042301 39.0 35.0 41.0 22.5 41.0 50-51 33.55204743286173 38.0 33.5 40.0 19.0 40.5 52-53 33.62080886132482 38.0 33.5 39.5 19.5 40.5 54-55 34.10224945859631 38.0 34.0 40.0 19.0 41.0 56-57 34.04142106881936 38.0 34.0 40.0 18.0 41.0 58-59 33.85373831874142 38.0 34.0 40.0 16.0 41.0 60-61 33.54779572859876 38.0 34.0 40.0 13.0 41.0 62-63 33.15439010979751 37.5 33.0 40.0 8.5 41.0 64-65 32.74638239110834 37.0 33.0 40.0 7.0 41.0 66-67 32.33278157205093 36.0 32.0 39.0 2.0 41.0 68-69 31.904147320789058 36.0 32.0 39.0 2.0 41.0 70-71 31.442726020240492 35.0 31.0 38.5 2.0 40.0 72-73 30.98251076487491 35.0 31.0 37.5 2.0 40.0 74-75 30.511856635460468 35.0 31.0 37.0 2.0 39.0 76-77 30.070616842078653 35.0 30.0 36.5 2.0 39.0 78-79 29.66715575573214 35.0 30.0 36.0 2.0 39.0 80-81 29.225768498310483 34.0 29.0 36.0 2.0 37.5 82-83 28.74376806434488 34.0 29.0 35.0 2.0 37.0 84-85 28.302891664489817 34.0 29.0 35.0 2.0 36.5 86-87 27.844133143174943 34.0 27.5 35.0 2.0 36.0 88-89 27.33043320721647 34.0 26.0 35.0 2.0 36.0 90-91 26.838620094714045 34.0 25.0 35.0 2.0 35.0 92-93 26.35296360587497 33.0 24.5 35.0 2.0 35.0 94-95 25.825602706597117 33.0 22.5 35.0 2.0 35.0 96-97 25.183923470376584 33.0 18.0 35.0 2.0 35.0 98-99 24.37937388875313 32.5 2.0 35.0 2.0 35.0 100-101 21.44950409950031 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3335858778740466 1101 2 0.3793088217364051 1101 3 0.5357171533401015 1101 4 0.38149089359702515 1101 5 0.2895319929303284 1101 6 0.5148337199331152 1101 7 0.4411600991101352 1101 8 0.537771854309014 1101 9 0.631451085437071 1101 10-11 0.5184773495626303 1101 12-13 0.5371257953360669 1101 14-15 0.7344978759635197 1101 16-17 0.5507694876612561 1101 18-19 0.8773042565707314 1101 20-21 0.8179245233924206 1101 22-23 0.7120559932966799 1101 24-25 0.6317775321778072 1101 26-27 0.7070052852415216 1101 28-29 0.5977495872344463 1101 30-31 0.715650547003662 1101 32-33 0.7767753395192969 1101 34-35 0.8199057609536808 1101 36-37 0.6493449472177701 1101 38-39 0.7816004911217078 1101 40-41 0.7497938135388011 1101 42-43 0.7270380026014749 1101 44-45 1.0366822343835551 1101 46-47 0.5228934733822825 1101 48-49 0.6952898558231979 1101 50-51 0.45957954111823085 1101 52-53 0.4974972468718164 1101 54-55 0.5896250618649361 1101 56-57 0.5767628645744836 1101 58-59 0.4733831835737021 1101 60-61 0.4879784802417504 1101 62-63 0.43962050069979597 1101 64-65 0.477386274838139 1101 66-67 0.23946054575490905 1101 68-69 0.3120135990050166 1101 70-71 0.08934356401764632 1101 72-73 0.21092304140716323 1101 74-75 0.09873307360771832 1101 76-77 0.09534762851014378 1101 78-79 0.21182914224969807 1101 80-81 0.028104292456962554 1101 82-83 -0.06301508886502205 1101 84-85 0.06148353217477265 1101 86-87 -0.07451293021031447 1101 88-89 -0.07837754647540862 1101 90-91 0.08896064275354831 1101 92-93 -0.2064947300576101 1101 94-95 -0.2853998861179363 1101 96-97 -0.8861299888051555 1101 98-99 -1.0506860947762249 1101 100-101 -0.5269930923075457 1102 1 0.2758813374989195 1102 2 0.29302278326098374 1102 3 0.27479601248704455 1102 4 0.07119897549912935 1102 5 0.1381446083308191 1102 6 0.13505652667920742 1102 7 0.21244364476316946 1102 8 0.30266431910864355 1102 9 0.4266657260408451 1102 10-11 0.34262592177712037 1102 12-13 0.4890401110133382 1102 14-15 0.6042973327001491 1102 16-17 0.3538687535680438 1102 18-19 0.26749972822992163 1102 20-21 0.3648519048974066 1102 22-23 0.4338999941906536 1102 24-25 0.3452104064879933 1102 26-27 0.37222138715497266 1102 28-29 0.36581180464293794 1102 30-31 0.32457184258363725 1102 32-33 0.37531488943591285 1102 34-35 0.4406249831930964 1102 36-37 0.5461378022730443 1102 38-39 0.5898831084132965 1102 40-41 0.5917037452870488 1102 42-43 0.34325080367424476 1102 44-45 0.6697882051229769 1102 46-47 0.8352118841650267 1102 48-49 0.8685388629922173 1102 50-51 0.6560483292627097 1102 52-53 0.6950061436689623 1102 54-55 0.7705545407791021 1102 56-57 0.8262041285213897 1102 58-59 0.8037724077042157 1102 60-61 0.7635965282758548 1102 62-63 0.72761240332499 1102 64-65 0.6612577139702935 1102 66-67 0.8613595606602686 1102 68-69 0.7714858942927201 1102 70-71 0.8308636699196228 1102 72-73 0.8505318402106106 1102 74-75 0.5015989597631005 1102 76-77 0.49898714920056975 1102 78-79 0.3944735110498776 1102 80-81 0.5544207605574307 1102 82-83 0.5699318569977514 1102 84-85 0.38760057448398655 1102 86-87 0.34050761408456154 1102 88-89 0.22145741149044795 1102 90-91 0.16907264784234854 1102 92-93 0.3488889067123786 1102 94-95 0.1466943425684306 1102 96-97 0.49265652244693214 1102 98-99 0.585699226797864 1102 100-101 0.6678424673740615 1103 1 0.17923153616684218 1103 2 0.14308710021493098 1103 3 0.3795897694547108 1103 4 0.08001897378401424 1103 5 0.050849350634223356 1103 6 0.11206535954088537 1103 7 0.036328102928564476 1103 8 0.0439129255746451 1103 9 0.1478317209526594 1103 10-11 0.17143277081808606 1103 12-13 0.15062716758367145 1103 14-15 0.3383331500971707 1103 16-17 0.3475006604942621 1103 18-19 0.3560684616147043 1103 20-21 0.3095843463689789 1103 22-23 0.20887069424496474 1103 24-25 0.3970284323862785 1103 26-27 0.42928553259704927 1103 28-29 0.334245758277568 1103 30-31 0.37854019864228405 1103 32-33 0.3715144720903467 1103 34-35 0.43549463416642453 1103 36-37 0.24598487084920606 1103 38-39 0.7075952828980405 1103 40-41 0.5177856993790328 1103 42-43 0.6679099238183213 1103 44-45 0.39159951347274813 1103 46-47 0.7592485591214313 1103 48-49 0.7208992952021447 1103 50-51 0.6529639740044431 1103 52-53 0.6030929286788336 1103 54-55 0.5476827173219903 1103 56-57 0.6433052919436264 1103 58-59 0.6779098458884789 1103 60-61 0.7576886587392266 1103 62-63 0.477181479733531 1103 64-65 0.6111147874059597 1103 66-67 0.7007729900402495 1103 68-69 0.6593993093800528 1103 70-71 0.6140412132978312 1103 72-73 0.64534574982385 1103 74-75 0.4498852702567646 1103 76-77 0.5813099917240798 1103 78-79 0.6067830614029042 1103 80-81 0.6346068509441913 1103 82-83 0.8584712904368494 1103 84-85 0.7949312779685371 1103 86-87 0.827792866921655 1103 88-89 0.7636321820933105 1103 90-91 0.6908410782228174 1103 92-93 0.6659143190555206 1103 94-95 0.952347802820551 1103 96-97 0.770535063452563 1103 98-99 0.6407652588705872 1103 100-101 0.6932319422618676 1104 1 0.1761864481015074 1104 2 0.3165671514277726 1104 3 0.3876869354466166 1104 4 0.28855214102163274 1104 5 0.17477059358380131 1104 6 0.2914002846247996 1104 7 0.2639691977761345 1104 8 0.296994347077117 1104 9 0.21045672527807113 1104 10-11 0.28863405805985565 1104 12-13 0.3838532307692546 1104 14-15 0.423907045387665 1104 16-17 0.5009765593142887 1104 18-19 0.5589820572408541 1104 20-21 0.5544336491130153 1104 22-23 0.6288402433643157 1104 24-25 0.6795226363379783 1104 26-27 0.6666086233614266 1104 28-29 0.310071911431649 1104 30-31 0.4359435690011253 1104 32-33 0.4678015408470557 1104 34-35 0.5404152196538092 1104 36-37 0.43877700898547545 1104 38-39 0.5649848119474967 1104 40-41 0.4728568091060126 1104 42-43 0.4746644827996036 1104 44-45 0.574612766526478 1104 46-47 0.7104025782916423 1104 48-49 0.756506063602437 1104 50-51 0.6139176402212669 1104 52-53 0.48447982787949684 1104 54-55 0.5800956035810287 1104 56-57 0.5421080186815743 1104 58-59 0.3528570758964378 1104 60-61 0.40134760887590915 1104 62-63 0.45825694947297535 1104 64-65 0.5651097353280363 1104 66-67 0.23987468906097575 1104 68-69 0.24738851315872878 1104 70-71 0.5352114960065677 1104 72-73 0.325569979035933 1104 74-75 0.32544843853248295 1104 76-77 0.3448554431237838 1104 78-79 0.32752080319327703 1104 80-81 0.24034753401280895 1104 82-83 0.6509450784571982 1104 84-85 0.6416941417354494 1104 86-87 0.493546007168721 1104 88-89 0.5023117211777084 1104 90-91 0.41356158758300055 1104 92-93 0.41703027462491704 1104 94-95 0.465337871681065 1104 96-97 0.6048545900797784 1104 98-99 0.6717213473165522 1104 100-101 1.0157448319812303 1105 1 0.11200365434736526 1105 2 0.11686859231521751 1105 3 0.01691464389298858 1105 4 0.029650591615471455 1105 5 0.1448555999140595 1105 6 0.1138154290149842 1105 7 -0.035638929070188397 1105 8 0.1784798477704328 1105 9 0.3016968530890054 1105 10-11 0.29821384946276197 1105 12-13 0.29500654360460743 1105 14-15 0.3193914608937902 1105 16-17 0.23755942541425412 1105 18-19 0.2728784821519312 1105 20-21 0.16590648928905694 1105 22-23 0.05584331806137044 1105 24-25 0.03791173623548616 1105 26-27 0.03621735308952623 1105 28-29 0.04057772924078051 1105 30-31 0.3080060275530059 1105 32-33 0.30978622764997255 1105 34-35 0.3698358502681458 1105 36-37 0.3084641133443071 1105 38-39 0.20483529726732286 1105 40-41 0.23696122822941845 1105 42-43 0.17841440743938364 1105 44-45 0.2778924433818517 1105 46-47 0.22140517068327625 1105 48-49 0.31682438517957934 1105 50-51 0.3623833061041921 1105 52-53 0.26717109212258094 1105 54-55 0.37986650850657355 1105 56-57 0.4277654612570885 1105 58-59 0.4600675419240474 1105 60-61 0.4296468793889545 1105 62-63 0.5869119202331987 1105 64-65 0.6085533960261742 1105 66-67 0.4687105243536749 1105 68-69 0.3671268624684281 1105 70-71 0.2732412783662532 1105 72-73 0.11791940931265543 1105 74-75 -0.07955548019905123 1105 76-77 -0.05462607854981627 1105 78-79 -0.17128549089245126 1105 80-81 0.1711969944336218 1105 82-83 0.27070754303789357 1105 84-85 0.44057881045191394 1105 86-87 0.33525336835519326 1105 88-89 0.3946732090899303 1105 90-91 0.45589594581534953 1105 92-93 0.4427431823237171 1105 94-95 0.44707658271983064 1105 96-97 0.4113837997017775 1105 98-99 0.5560102482630782 1105 100-101 0.4350370487775166 1106 1 0.2681139793479268 1106 2 0.21833300396761857 1106 3 0.3104595532656731 1106 4 0.3451167551713894 1106 5 0.24894786683228176 1106 6 0.15021234746162548 1106 7 0.1861340871236905 1106 8 0.2592807821189709 1106 9 0.35782514633070406 1106 10-11 0.3638203899025285 1106 12-13 0.2872072039374629 1106 14-15 0.2797377987416425 1106 16-17 0.40716277649121935 1106 18-19 0.5222832841643736 1106 20-21 0.36718448043969687 1106 22-23 0.3945895840362539 1106 24-25 0.3424397330011999 1106 26-27 0.5697561981808335 1106 28-29 0.33709862855125294 1106 30-31 0.250580651904464 1106 32-33 0.4778061274770309 1106 34-35 0.2972722314643832 1106 36-37 0.4748107207157872 1106 38-39 0.5628749621596256 1106 40-41 0.47745490565456805 1106 42-43 0.24705870364582694 1106 44-45 0.41461391318397744 1106 46-47 0.38291719817468817 1106 48-49 0.37747842915684515 1106 50-51 0.21079873183920483 1106 52-53 0.2581869715556735 1106 54-55 0.30907163843941987 1106 56-57 0.4507423496069265 1106 58-59 0.578163806775926 1106 60-61 0.5734264989114592 1106 62-63 0.6451907873356006 1106 64-65 0.32572205042951197 1106 66-67 0.5039900428048156 1106 68-69 0.36951326949401064 1106 70-71 0.37105027596300033 1106 72-73 0.45277442806700563 1106 74-75 0.5738259181793168 1106 76-77 0.2668426005598583 1106 78-79 0.3630843853512893 1106 80-81 0.4083328567968927 1106 82-83 0.6968973775054721 1106 84-85 0.6125461082530528 1106 86-87 0.86809594410256 1106 88-89 0.7257436670554753 1106 90-91 0.5457539967103955 1106 92-93 0.458768607384922 1106 94-95 0.38739956185420965 1106 96-97 0.3963578580536371 1106 98-99 0.6267265072752721 1106 100-101 1.0943367365793293 1107 1 -0.016494672458257753 1107 2 0.027596544415338542 1107 3 0.12429920848729381 1107 4 0.09694101714206482 1107 5 0.04982741321658324 1107 6 0.047166519717627864 1107 7 0.05466354645715654 1107 8 -0.02919758836556241 1107 9 0.07847046413759529 1107 10-11 0.13723016623421813 1107 12-13 0.1437481463697523 1107 14-15 0.06232477822047855 1107 16-17 0.08344546812197251 1107 18-19 0.13607608415676253 1107 20-21 0.05074994932496679 1107 22-23 -0.007641152994118272 1107 24-25 -0.052446387385813864 1107 26-27 -0.06026706294312589 1107 28-29 -0.06940854006098363 1107 30-31 0.04198687960661118 1107 32-33 0.05897151454403371 1107 34-35 0.05849726975723257 1107 36-37 0.24226726665293796 1107 38-39 0.3206227240494357 1107 40-41 0.16304680374325642 1107 42-43 0.03472602676139758 1107 44-45 -0.17779791811816636 1107 46-47 -0.028654854227056603 1107 48-49 0.009273626614771047 1107 50-51 -0.10678577406407896 1107 52-53 -0.08756299752462837 1107 54-55 -0.15939978700606616 1107 56-57 -0.015939301219340507 1107 58-59 -0.27185308013410747 1107 60-61 0.06334548931208417 1107 62-63 0.023107941582061642 1107 64-65 0.19329418845503454 1107 66-67 0.12322589823384078 1107 68-69 0.15070767350500702 1107 70-71 0.0792313892622758 1107 72-73 0.2583383081556754 1107 74-75 0.25802524080016553 1107 76-77 0.33584346631129947 1107 78-79 0.3140302901566834 1107 80-81 0.4135356899922762 1107 82-83 0.4975129172681996 1107 84-85 0.7204487064587575 1107 86-87 0.7921536069542121 1107 88-89 0.8475886690057948 1107 90-91 0.665104017164964 1107 92-93 0.7056516643437547 1107 94-95 0.8900729262023788 1107 96-97 0.8479768608221399 1107 98-99 0.7556330917431211 1107 100-101 0.6660097774599194 1108 1 0.27694825545996693 1108 2 0.2617301336216791 1108 3 0.3073234707310135 1108 4 0.22148818067233123 1108 5 0.09185553313746908 1108 6 0.12659158874458853 1108 7 0.21466413392068517 1108 8 0.3913430739016519 1108 9 0.34262833516976343 1108 10-11 0.29292758335290614 1108 12-13 0.2106259735379794 1108 14-15 0.21196397770351183 1108 16-17 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-0.6031381575704238 2316 84-85 -0.6149786275611113 2316 86-87 -0.5310860330798057 2316 88-89 -0.649727374095395 2316 90-91 -0.8020524955569215 2316 92-93 -0.6114118248719755 2316 94-95 -0.9158838075512534 2316 96-97 -1.0394346457460202 2316 98-99 -0.8225138297798438 2316 100-101 -0.9383662743246575 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3032.0 3 633.0 4 711.0 5 719.0 6 954.0 7 1132.0 8 1463.0 9 1822.0 10 2410.0 11 3345.0 12 4962.0 13 7394.0 14 6962.0 15 8016.0 16 10872.0 17 15128.0 18 12157.0 19 7146.0 20 6909.0 21 7104.0 22 7485.0 23 8319.0 24 9341.0 25 10405.0 26 11971.0 27 14017.0 28 16222.0 29 19290.0 30 22772.0 31 26987.0 32 33288.0 33 41827.0 34 56499.0 35 82429.0 36 116987.0 37 176389.0 38 165173.0 39 27106.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.23730694241164 20.16538357894292 15.321705405876665 20.275604072768775 2 15.12457804550467 22.53843185790702 40.545478313489255 21.79151178309905 3 18.79529682666537 28.3012780637366 31.993345043470196 20.910080066127833 4 11.934733900941035 18.322482009647572 39.11031446872447 30.63246962068693 5 11.86812792822623 39.27637815784451 34.627807485503205 14.22768642842606 6 27.14655047938846 38.33431877514325 18.59502346616246 15.924107279305828 7 24.831522964942863 36.35119952276258 22.31854332974281 16.498734182551743 8 24.5513481951165 33.985815572936815 21.368442021233427 20.094394210713258 9 26.8287798507738 17.545409223186873 22.405853528970944 33.21995739706839 10-11 23.521776592633188 27.48469749389349 27.77723029250645 21.21629562096687 12-13 26.483852710326865 26.14479177316117 28.042510674043026 19.328844842468936 14-15 23.371122356652286 26.65161640162707 28.69357270421539 21.28368853750525 16-17 19.479262949655833 30.107692907634732 29.63757721522456 20.775466927484878 18-19 19.24883173445075 28.469262507832795 33.08398389224394 19.197921865472516 20-21 20.994933157095318 27.0489513732757 32.373194535356696 19.582920934272284 22-23 20.541445171560426 27.022763151999452 32.08088996887428 20.354901707565844 24-25 20.388552186067304 27.53139050211625 32.5801756571328 19.499881654683655 26-27 20.473668285657958 26.991048281925877 33.106122823349324 19.42916060906684 28-29 19.539944515868534 27.148586883585057 33.61976765822281 19.6917009423236 30-31 19.74112676323593 27.842867097261152 32.92819712189717 19.48780901760574 32-33 19.377897756529684 28.100115264631675 32.50188550169103 20.020101477147616 34-35 20.200362470080364 27.360573188838455 32.388551017928 20.050513323153176 36-37 20.69689063357688 27.13026880158038 32.307905670394774 19.864934894447963 38-39 20.59875543287577 27.09195815316357 32.64324190737665 19.66604450658401 40-41 19.95347217960141 27.310200989179133 33.18771123757441 19.548615593645042 42-43 19.543965666435383 28.12449886555069 32.038679773036044 20.292855694977884 44-45 19.72086469697443 28.32750125313416 31.34275111511738 20.608882934774037 46-47 20.180143560169412 28.162859601711038 30.48229526903506 21.174701569084494 48-49 20.455134985193396 28.25652639302242 30.318651662076988 20.969686959707193 50-51 20.771756196356563 28.651459376822963 30.28668769261879 20.290096734201686 52-53 20.22419153257717 28.884563487673226 30.461823334827876 20.42942164492173 54-55 19.666364439447904 28.513417882853343 30.658066687944306 21.162150989754444 56-57 20.37400210189647 29.319127363707466 29.358074891439056 20.94879564295701 58-59 20.24411433554002 28.308278871713256 30.010962870155595 21.43664392259113 60-61 20.395890992731545 28.28139602162713 30.27364998336659 21.049063002274732 62-63 20.978358492932678 28.067856029544778 30.291017962170365 20.662767515352183 64-65 20.591494076536264 28.192182901864626 30.527952957443915 20.688370064155194 66-67 19.981078153473437 28.48251449845459 30.168591852487953 21.36781549558402 68-69 19.7212608865092 28.85454437629198 30.329306313328136 21.094888423870685 70-71 20.181966729623788 28.628652002793626 29.54060363647531 21.648777631107276 72-73 19.988956202288914 28.252004004567876 29.498004863294618 22.261034929848588 74-75 19.814721576761947 28.905096427264876 29.38555181956266 21.894630176410516 76-77 20.290877602858597 28.04743724848715 29.896994522359137 21.76469062629511 78-79 19.7374694724253 27.883112793931264 30.364584573619414 22.014833160024025 80-81 19.762244468106275 28.91947874351446 29.593911476999217 21.724365311380048 82-83 19.717519686081232 28.408399253522003 29.462063738550636 22.412017321846125 84-85 19.654838605529758 27.7075459265144 29.5482055725247 23.08940989543114 86-87 19.88463928340438 27.878698704017935 29.64811699935111 22.588545013226575 88-89 20.369774409027876 27.169314969882386 29.803309232186148 22.657601388903593 90-91 19.764731541865473 27.193618180287782 30.20676117567527 22.834889102171477 92-93 19.720838384854435 27.72117721177212 29.817351114687618 22.740633288685828 94-95 19.48198269745968 27.404566006967606 29.768840602729863 23.34461069284285 96-97 19.541714048540214 26.85424594527632 29.427955199512102 24.17608480667137 98-99 19.741533118385455 26.877379118290158 29.248000084708526 24.133087678615865 100-101 20.279143968377305 26.23491750278943 30.04304294343442 23.44289558539885 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4149.0 1 2618.0 2 1171.5 3 1231.0 4 1032.0 5 1015.5 6 1603.0 7 2917.0 8 4369.0 9 4734.5 10 3847.0 11 3112.0 12 3039.5 13 3027.0 14 3088.5 15 3220.5 16 3332.0 17 3313.0 18 3269.5 19 3328.0 20 3535.0 21 3876.5 22 4254.5 23 4722.5 24 5288.5 25 6290.0 26 7642.5 27 8902.0 28 9576.0 29 10674.0 30 12784.5 31 14858.5 32 17216.0 33 20202.0 34 23595.5 35 26181.5 36 29599.0 37 32677.0 38 32663.5 39 31439.0 40 29619.0 41 28349.0 42 27478.5 43 28073.0 44 29576.5 45 30094.5 46 30474.0 47 33042.0 48 48533.0 49 48565.0 50 32232.5 51 26324.5 52 22567.5 53 21289.0 54 20604.5 55 19195.0 56 17316.0 57 15500.0 58 14457.0 59 13053.5 60 11284.0 61 9604.5 62 7944.5 63 6052.0 64 4266.0 65 3249.5 66 2394.0 67 1885.0 68 1428.0 69 911.5 70 642.0 71 485.5 72 375.5 73 305.0 74 255.5 75 198.0 76 140.0 77 88.0 78 59.5 79 40.0 80 23.5 81 14.0 82 13.5 83 14.5 84 11.0 85 9.5 86 7.5 87 4.0 88 3.0 89 2.5 90 1.5 91 1.0 92 0.5 93 0.0 94 1.0 95 1.0 96 1.0 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32642218533280526 2 0.08658245767782057 3 0.09669427755260253 4 0.10101286729079066 5 0.11280999047803629 6 0.06014426196354689 7 0.06214555964709748 8 0.06288287984630034 9 0.06562149772905379 10-11 0.06335687140293074 12-13 0.061829565276010544 14-15 0.05935427603582955 16-17 0.0605655877916628 18-19 0.06830744988329274 20-21 0.07025608183832885 22-23 0.08547647737901629 24-25 0.09358699957024764 26-27 0.08963706993166094 28-29 0.08684578632039301 30-31 0.08142121628340061 32-33 0.0734686912777127 34-35 0.32642218533280526 36-37 0.5058543223816706 38-39 0.4800481154095708 40-41 0.45903448973228955 42-43 0.49005460382732385 44-45 0.5005877495302218 46-47 0.4943205278369975 48-49 0.5132801901022136 50-51 0.5094882576491705 52-53 0.509224929006598 54-55 0.4982704574755842 56-57 0.47641418014207104 58-59 0.46093045595881116 60-61 0.42106249947334273 62-63 0.4896332779992079 64-65 0.5137541816588441 66-67 0.5238133358051116 68-69 0.5367691050196759 70-71 0.5361897820060165 72-73 0.5227073555063072 74-75 0.5232340127914521 76-77 0.4977438001904393 78-79 0.4783628120971072 80-81 0.4834713877630127 82-83 0.4930565503526497 84-85 0.4945838564795699 86-87 0.49453119075105545 88-89 0.5007457467157652 90-91 0.5149128276861629 92-93 0.5212853808364161 94-95 0.5281319255432997 96-97 0.5192840831528654 98-99 0.5233920099769955 100-101 0.49990309505953334 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 949384.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.77262266998743 #Duplication Level Percentage of deduplicated Percentage of total 1 72.70651804767911 30.371419440543306 2 13.12669250115889 10.966727455117276 3 5.25268848478784 6.582557222340912 4 2.605062257349779 4.35281130832392 5 1.5212294685309178 3.1772872291703766 6 0.9168500477394228 2.2979538653507317 7 0.6424594334081885 1.878605084477392 8 0.4809216098130532 1.6071485552450877 9 0.3511345862768445 1.3201031321032295 >10 2.0941389749336476 16.59017939442892 >50 0.19977391564334726 5.811967593155447 >100 0.0919501118663842 6.773835559526686 >500 0.005038362291738803 1.4573690948924805 >1k 0.004786444177151862 4.429353929750841 >5k 7.557543437608203E-4 2.3826811355733657 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9231 0.9723146798345033 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7362 0.7754501866473419 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 6049 0.6371499835682928 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4973 0.5238133358051116 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4600 0.4845247023333024 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3611 0.38035189133164243 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3352 0.35307104396113687 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3175 0.3344273760670076 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2412 0.25405947435389686 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2283 0.24047171639715856 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2122 0.22351335181549298 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2048 0.21571882399534856 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1748 0.18411938688665494 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1628 0.17147961204317747 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1599 0.1684249997893371 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1458 0.15357326434825108 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1407 0.14820136003977316 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1384 0.14577873652810666 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1209 0.12734573154803536 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1047 0.1102820355093408 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1029 0.10838606928281919 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1006 0.10596344577115267 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 999 0.10522612557194982 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.0533145702897879E-4 0.0 5 0.0 0.0 0.0 1.0533145702897879E-4 0.0 6 0.0 0.0 0.0 1.0533145702897879E-4 0.0 7 0.0 0.0 0.0 1.0533145702897879E-4 0.0 8 0.0 0.0 0.0 1.0533145702897879E-4 0.0 9 0.0 0.0 0.0 3.1599437108693635E-4 0.0 10-11 0.0 0.0 0.0 3.686600996014258E-4 0.0 12-13 0.0 0.0 0.0 4.2132582811591515E-4 0.0 14-15 0.0 0.0 0.0 7.373201992028516E-4 0.0 16-17 0.0 0.0 0.0 7.373201992028516E-4 0.0 18-19 0.0 0.0 0.0 8.426516562318304E-4 0.0 20-21 0.0 0.0 0.0 0.001211311755833256 0.0 22-23 0.0 0.0 0.0 0.0027386178827534485 0.0 24-25 0.0 0.0 0.0 0.00437125546670262 0.0 26-27 0.0 0.0 0.0 0.007215204806485047 0.0 28-29 0.0 0.0 0.0 0.017274358952752523 0.0 30-31 0.0 0.0 0.0 0.0467145011923521 0.0 32-33 0.0 0.0 0.0 0.09495630851162437 0.0 34-35 0.0 0.0 0.0 0.1724275951564383 0.0 36-37 0.0 0.0 0.0 0.28692288894693824 0.0 38-39 0.0 0.0 0.0 0.44428808574823253 0.0 40-41 0.0 0.0 0.0 0.6220875852131487 0.0 42-43 0.0 0.0 0.0 0.7908812451020872 0.0 44-45 0.0 0.0 0.0 0.9926963167696106 0.0 46-47 0.0 0.0 0.0 1.2344320106511169 0.0 48-49 0.0 0.0 0.0 1.4942847151416077 0.0 50-51 0.0 0.0 0.0 1.7621426103663007 0.0 52-53 0.0 0.0 0.0 2.013042141009328 0.0 54-55 0.0 0.0 0.0 2.2880099095834776 0.0 56-57 0.0 0.0 0.0 2.596631078678385 0.0 58-59 0.0 0.0 0.0 2.965185846822782 0.0 60-61 0.0 0.0 0.0 3.4019427333934424 0.0 62-63 0.0 0.0 0.0 3.8132094073630904 0.0 64-65 0.0 0.0 0.0 4.220947477522267 0.0 66-67 0.0 0.0 0.0 4.682878582322854 0.0 68-69 0.0 0.0 0.0 5.189522890632242 0.0 70-71 0.0 0.0 0.0 5.775165791713363 0.0 72-73 0.0 0.0 0.0 6.382190978571368 0.0 74-75 0.0 0.0 0.0 6.996536701692888 0.0 76-77 0.0 0.0 0.0 7.610619096171833 0.0 78-79 0.0 0.0 0.0 8.305438052463492 0.0 80-81 0.0 0.0 0.0 9.079255601526885 0.0 82-83 0.0 0.0 0.0 9.875087425109335 0.0 84-85 0.0 0.0 0.0 10.711577191104968 0.0 86-87 0.0 0.0 0.0 11.59141085166803 0.0 88-89 0.0 0.0 0.0 12.474141127299387 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 45 9.767726E-4 42.095425 2 ATCACGC 65 1.3517156E-4 36.42873 3 GAGTACT 6575 0.0 25.130585 12-13 GTATGGT 205 2.6904672E-8 23.152426 1 GTACATG 6410 0.0 23.10186 1 GTACTTT 7235 0.0 22.96897 14-15 AGTACTT 6850 0.0 22.255545 12-13 CGCAGAA 300 7.2759576E-12 22.094275 9 TACATGG 6830 0.0 21.494553 2 ACTTTTT 8195 0.0 20.856007 16-17 ACATGGG 6635 0.0 20.841518 3 GGATATG 355 3.6379788E-12 20.05457 1 CATGGGA 4350 0.0 19.922749 4 CATGGGG 1770 0.0 19.79912 4 GTTACAC 175 1.016638E-4 18.942942 3 GTAGTAC 125 0.0061360425 18.942942 3 ATGGGAG 1405 0.0 18.875528 5 TACTTTT 8155 0.0 18.781197 14-15 ACGCAGT 155 9.83656E-4 18.327047 7 TACCTGG 525 0.0 18.040895 2 >>END_MODULE