FastQCFastQC Report
Fri 27 May 2016
SRR938981_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938981_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences949384
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89270.9402939168976937No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76030.8008350677913257No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA60690.6392566127088722No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA51380.541193026214893No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49820.5247613189183723No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT32530.342643229715268No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31720.3341113816959207No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30740.3237888989070808No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25610.26975386145121466No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG23560.24816091276027405No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA21630.2278319415536811No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT20490.21582415545237754No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC18490.1947578640465818No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16600.1748502186681048No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG15500.16326375839491714No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14870.15662787660209146No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT14280.15041332063738172No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT13720.1445147590437589No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG13550.14272412427426626No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA11620.12239515306767335No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG10760.11333664776318117No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG9710.1022768447751384No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT9570.10080220437673271No Hit
GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA9570.10080220437673271No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCCG200.002082740271.1741945
GGACGTG1255.7098077E-934.1636166
TAGGACG1153.8957896E-628.8822844
CTTTGCG3450.024.7562452
GAGTACT65650.024.64950412-13
GTACTTT70400.022.91955614-15
GTACATG70200.022.860321
TTTGCGT3850.022.1841663
TACATGG73050.021.8248062
GTATAGG5050.021.6883221
AACGGCG550.002894111221.57021158-59
AGTACTT68950.021.54262412-13
ACATGGG72350.020.9866333
CATGGGG18700.020.8067174
CTGGTCG2551.2050805E-820.4694739
ACTTTTT80050.020.24393816-17
GTCGGGA3353.45608E-1119.8296282
ACGCAGT1456.189164E-419.6342627
CCTATAC3900.019.4664483
GTGTAAC1250.00594311919.0480041
Produced by FastQC (version 0.11.5)