##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938981_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 949384 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16061888550892 33.0 31.0 34.0 30.0 34.0 2 32.38306417635014 34.0 31.0 34.0 30.0 34.0 3 32.446326249441746 34.0 31.0 34.0 30.0 34.0 4 36.01306952718816 37.0 35.0 37.0 35.0 37.0 5 35.9493766484373 37.0 35.0 37.0 35.0 37.0 6 36.009718933540064 37.0 35.0 37.0 35.0 37.0 7 35.94932292939422 37.0 35.0 37.0 35.0 37.0 8 35.934401675191495 37.0 35.0 37.0 35.0 37.0 9 37.58517417609734 39.0 37.0 39.0 35.0 39.0 10-11 37.50562891306363 39.0 37.0 39.0 35.0 39.0 12-13 37.426473376420915 39.0 37.0 39.0 34.0 39.0 14-15 38.636180934163626 40.0 38.0 41.0 34.0 41.0 16-17 38.70909505532008 40.0 38.0 41.0 34.0 41.0 18-19 38.82248226218263 40.0 38.0 41.0 35.0 41.0 20-21 38.82392951640222 40.0 38.0 41.0 34.0 41.0 22-23 38.742071701229435 40.0 38.0 41.0 34.0 41.0 24-25 38.648919720576714 40.0 38.0 41.0 34.0 41.0 26-27 38.443580258357 40.0 38.0 41.0 34.0 41.0 28-29 38.25665273482595 40.0 38.0 41.0 34.0 41.0 30-31 37.995432828023226 40.0 37.0 41.0 33.0 41.0 32-33 37.78972417904662 40.0 36.5 41.0 33.0 41.0 34-35 37.64757463787045 40.0 36.0 41.0 33.0 41.0 36-37 37.455868226134 40.0 36.0 41.0 33.0 41.0 38-39 37.24623966698407 40.0 36.0 41.0 32.5 41.0 40-41 37.00722363132304 40.0 35.0 41.0 31.0 41.0 42-43 36.760884426112085 40.0 35.0 41.0 31.0 41.0 44-45 36.408874069923236 39.5 35.0 41.0 30.5 41.0 46-47 35.9068190531966 39.0 35.0 41.0 29.0 41.0 48-49 35.4290692701794 39.0 35.0 41.0 26.5 41.0 50-51 35.120156859605814 39.0 35.0 40.5 25.5 41.0 52-53 34.82233690477193 38.5 35.0 40.0 24.0 41.0 54-55 34.532034456026224 38.0 34.0 40.0 23.5 41.0 56-57 34.44272496692592 38.0 34.0 40.0 23.5 41.0 58-59 34.197242633117895 38.0 34.0 40.0 23.0 41.0 60-61 33.850497796465916 38.0 34.0 40.0 22.0 41.0 62-63 33.422044188652855 37.0 33.0 40.0 20.5 41.0 64-65 32.98872110758134 36.5 33.0 39.5 18.5 41.0 66-67 32.543789446630655 36.0 32.0 39.0 16.5 40.0 68-69 32.08434100427225 35.5 32.0 39.0 12.0 40.0 70-71 31.56472249374331 35.0 31.0 38.0 8.0 40.0 72-73 31.065957505076973 35.0 31.0 37.0 6.5 39.0 74-75 30.550083527845423 35.0 30.5 37.0 2.0 39.0 76-77 28.622311941216623 32.5 28.0 35.0 2.0 37.0 78-79 29.552581463348865 34.0 29.0 35.5 2.0 37.5 80-81 29.548291313104073 34.0 30.0 35.0 2.0 37.0 82-83 29.148319331271647 34.0 29.0 35.0 2.0 37.0 84-85 28.716960155216434 34.0 29.0 35.0 2.0 36.0 86-87 28.044334536920783 34.0 27.5 35.0 2.0 36.0 88-89 27.36130006404153 33.0 26.0 35.0 2.0 35.0 90-91 26.793661995567653 33.0 25.5 35.0 2.0 35.0 92-93 26.44199501992871 33.0 25.0 34.5 2.0 35.0 94-95 25.948611942059273 32.0 24.5 34.0 2.0 35.0 96-97 25.17390065558299 32.0 20.5 34.0 2.0 35.0 98-99 24.099656198124258 31.5 4.5 34.0 2.0 35.0 100-101 18.96201958322449 24.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12093083974236407 1101 2 0.17989309191683134 1101 3 0.22033204856149524 1101 4 0.2194424691528596 1101 5 0.21994381663834872 1101 6 0.1582140361958082 1101 7 0.12470597130473493 1101 8 0.21493428450277463 1101 9 0.35831690070808975 1101 10-11 0.4351211166138782 1101 12-13 0.42540695340353807 1101 14-15 0.3455121561961221 1101 16-17 0.4348393291576045 1101 18-19 0.45083210220499836 1101 20-21 0.43082994069499847 1101 22-23 0.37714415346669483 1101 24-25 0.3142636406623396 1101 26-27 0.4059621222241958 1101 28-29 0.36007868035463275 1101 30-31 0.5451979392500945 1101 32-33 0.594524338789995 1101 34-35 0.37244304759732216 1101 36-37 0.5676409442025374 1101 38-39 0.46307408423725605 1101 40-41 0.45345009882098 1101 42-43 0.6125603129937005 1101 44-45 0.7793858251738754 1101 46-47 0.9089830006702115 1101 48-49 0.739995897594234 1101 50-51 0.5309709619094392 1101 52-53 0.5176131718173025 1101 54-55 0.522101680594723 1101 56-57 0.6621814845398291 1101 58-59 0.39923928624032357 1101 60-61 0.5396088305465838 1101 62-63 0.569759158016403 1101 64-65 0.6072187037279448 1101 66-67 0.3394138695756155 1101 68-69 0.44952915788952197 1101 70-71 0.607621527749302 1101 72-73 0.5936762617203364 1101 74-75 0.610580260402056 1101 76-77 0.31414750184670126 1101 78-79 0.6670368744001145 1101 80-81 0.6747967088373237 1101 82-83 0.5481695928760217 1101 84-85 0.5978847768821431 1101 86-87 0.8239579608752017 1101 88-89 0.8793424543756956 1101 90-91 0.7524036768545699 1101 92-93 0.5891045367370467 1101 94-95 0.31624842249151897 1101 96-97 0.3800079066622075 1101 98-99 0.43218817703417045 1101 100-101 1.0996759394606563 1102 1 0.2486707953873193 1102 2 0.202822759941661 1102 3 0.13026809477326395 1102 4 0.07072412369925019 1102 5 0.12342001448249107 1102 6 0.1315021548885369 1102 7 0.13147097551674136 1102 8 0.09854956464443632 1102 9 0.1753910148965474 1102 10-11 0.199884642324335 1102 12-13 0.10296037716615558 1102 14-15 0.18212054239447895 1102 16-17 0.26685533479653856 1102 18-19 0.23021732062780842 1102 20-21 0.19239147902428755 1102 22-23 0.11791426366664126 1102 24-25 0.16222941163213278 1102 26-27 0.04536461848496032 1102 28-29 0.16026392284608448 1102 30-31 0.11870181601870655 1102 32-33 0.09036605316411794 1102 34-35 0.03473653015615952 1102 36-37 -0.06085975206854499 1102 38-39 -0.06429108845516396 1102 40-41 -0.19981549851083713 1102 42-43 -0.1824136413102906 1102 44-45 -0.10678029994861049 1102 46-47 -0.04941050423806104 1102 48-49 -0.09785245284501087 1102 50-51 -0.0922024009939193 1102 52-53 -0.020503371927432568 1102 54-55 0.1517606796835551 1102 56-57 -0.1766639948557014 1102 58-59 -0.11397267243039977 1102 60-61 -0.2320335955713233 1102 62-63 -0.21880867254044034 1102 64-65 -0.21643560197804135 1102 66-67 -0.34705139423375897 1102 68-69 -0.19673504690013033 1102 70-71 -0.17324863918383926 1102 72-73 -0.10605898334989305 1102 74-75 -0.060362886055557397 1102 76-77 -0.2650683765723336 1102 78-79 -0.02425715829884112 1102 80-81 0.11850834945829192 1102 82-83 -0.04451182442244672 1102 84-85 -0.0249103646929818 1102 86-87 0.16708421803132012 1102 88-89 -0.00516895243572435 1102 90-91 0.043004447050726924 1102 92-93 0.16304680640885394 1102 94-95 0.23044375661567784 1102 96-97 0.07981406823148163 1102 98-99 0.34676603045621235 1102 100-101 1.037396210404662 1103 1 0.18171684632634566 1103 2 0.028179361719665508 1103 3 0.11927847124183444 1103 4 0.009365749631847109 1103 5 0.03484270550384139 1103 6 -0.051708261599472394 1103 7 -0.024659144827424484 1103 8 0.05475543606770117 1103 9 0.08146682314627185 1103 10-11 0.08309362954669552 1103 12-13 0.12149938182555786 1103 14-15 0.12705665422022605 1103 16-17 0.08645128139917091 1103 18-19 0.18261419911164722 1103 20-21 0.1591024529258931 1103 22-23 0.16197923808278603 1103 24-25 0.21306195886936763 1103 26-27 0.19911000910326493 1103 28-29 0.11943909364332939 1103 30-31 0.029598537283327175 1103 32-33 0.10591574873053844 1103 34-35 0.027846755122574507 1103 36-37 0.10525807659109176 1103 38-39 0.3742233328351787 1103 40-41 0.2752495330759288 1103 42-43 -0.08613973684240506 1103 44-45 0.09772831852321673 1103 46-47 0.2801545702266779 1103 48-49 0.16476724815260724 1103 50-51 0.010811205615006259 1103 52-53 -0.07342264700390189 1103 54-55 -0.07353660757025438 1103 56-57 -0.10751254986813308 1103 58-59 -0.23497144326194785 1103 60-61 -0.09117303472747551 1103 62-63 0.16537007142091653 1103 64-65 -0.04067308875733033 1103 66-67 -0.1688870000994669 1103 68-69 -0.07155853259317979 1103 70-71 -0.05461744689043968 1103 72-73 -0.022358328746225453 1103 74-75 0.037766205217348414 1103 76-77 -0.5586981396000859 1103 78-79 -0.31357196834943935 1103 80-81 -0.2573363465369063 1103 82-83 -0.10820276001973994 1103 84-85 0.05191623679917612 1103 86-87 0.17727416743531776 1103 88-89 -0.014365562217619754 1103 90-91 0.037276664985153474 1103 92-93 0.048516891878939816 1103 94-95 0.2112458604947065 1103 96-97 0.22703914898498923 1103 98-99 0.2810212401294834 1103 100-101 0.806550513923515 1104 1 0.1281648203046828 1104 2 0.1048186918002898 1104 3 0.07724241463088077 1104 4 0.08483548906919935 1104 5 0.08723898219162152 1104 6 0.13329467967877662 1104 7 0.1594648733046924 1104 8 0.1715345633731431 1104 9 0.12834041738828716 1104 10-11 0.1698820997359789 1104 12-13 0.07650474533294016 1104 14-15 0.18987543687705966 1104 16-17 0.1724680985900804 1104 18-19 0.2550838347391817 1104 20-21 0.17158039333906316 1104 22-23 0.14686798855857575 1104 24-25 0.1755589310829393 1104 26-27 0.033166550852115506 1104 28-29 0.1547655755285149 1104 30-31 0.08602955565766734 1104 32-33 0.13575761177078505 1104 34-35 0.28460304426752003 1104 36-37 0.3023998462024977 1104 38-39 0.3233392191980258 1104 40-41 0.2856408960988759 1104 42-43 0.18518107010593354 1104 44-45 0.2782120722863226 1104 46-47 0.27897113827400943 1104 48-49 0.5096926780982827 1104 50-51 0.554117202362292 1104 52-53 0.4386054508541619 1104 54-55 0.49591908293806597 1104 56-57 0.48282621617913435 1104 58-59 0.5197674750397212 1104 60-61 0.5933350797401502 1104 62-63 0.5677092527074663 1104 64-65 0.5577317003357152 1104 66-67 0.6042262564145986 1104 68-69 0.5788688178341701 1104 70-71 0.5736430465522417 1104 72-73 0.4553190995384888 1104 74-75 0.5285132898915563 1104 76-77 0.43189323034350835 1104 78-79 0.6059996431250063 1104 80-81 0.6772357839866423 1104 82-83 0.7729557575760246 1104 84-85 0.7077555391971835 1104 86-87 0.8085347646878169 1104 88-89 0.8377054436995373 1104 90-91 0.8545160158277412 1104 92-93 0.8010585023152892 1104 94-95 0.7491572069080732 1104 96-97 0.767666852365732 1104 98-99 0.5492450541022862 1104 100-101 0.5667567494333916 1105 1 0.003476811623187359 1105 2 -0.01595058534446281 1105 3 -0.0028685156157024494 1105 4 -0.00704369342311395 1105 5 -0.01849442348753172 1105 6 0.0920455086412133 1105 7 0.0671998464764556 1105 8 0.080607934085144 1105 9 -0.010611774086967785 1105 10-11 0.03884574596494872 1105 12-13 0.03133081394089032 1105 14-15 0.02028479360220814 1105 16-17 -0.03517041349770267 1105 18-19 0.0016202789424681896 1105 20-21 -0.006908784933081336 1105 22-23 -0.054946685583821875 1105 24-25 -0.14190052511769835 1105 26-27 -0.2714153506370067 1105 28-29 -0.09823376130152894 1105 30-31 -0.14406027372680086 1105 32-33 -0.003544251133902776 1105 34-35 0.007313600727400171 1105 36-37 -0.04713894923971651 1105 38-39 0.18508637058164368 1105 40-41 -0.00896934083366574 1105 42-43 -0.0787605709382575 1105 44-45 0.07605375553224292 1105 46-47 0.09008224938512654 1105 48-49 0.13458566658324145 1105 50-51 0.15694471788649622 1105 52-53 0.06013435903526698 1105 54-55 0.3540429148546238 1105 56-57 0.12109112243101094 1105 58-59 -0.016134134654613774 1105 60-61 -0.09803165324350971 1105 62-63 0.009470873253668799 1105 64-65 -0.1557005056086993 1105 66-67 1.0857389213470015E-4 1105 68-69 -0.010707356278082614 1105 70-71 0.11062381462362225 1105 72-73 0.030401995782536773 1105 74-75 -0.24442334723390147 1105 76-77 -0.2961829739128383 1105 78-79 -0.08578910710107479 1105 80-81 0.052475429492158554 1105 82-83 -0.2319215559827832 1105 84-85 -0.10732856281041236 1105 86-87 -0.23070078122163196 1105 88-89 -0.2051353352237122 1105 90-91 -0.19860992617503115 1105 92-93 0.0026802542897250703 1105 94-95 -0.0049514323877595245 1105 96-97 -0.16223608366851394 1105 98-99 0.038670507770820706 1105 100-101 0.21151469240077958 1106 1 0.03940206374007005 1106 2 -0.056412818347631344 1106 3 0.054607395711030904 1106 4 0.03392389636194082 1106 5 -0.039741868628240695 1106 6 -0.004545017603646784 1106 7 -0.09207738102394103 1106 8 0.07205055924517723 1106 9 -0.07707180598059438 1106 10-11 0.05318103334451507 1106 12-13 -0.14220870495945803 1106 14-15 -0.26076095803178134 1106 16-17 -0.1937513716541588 1106 18-19 -0.06948903534452455 1106 20-21 0.07912539964566889 1106 22-23 -0.08558815179230095 1106 24-25 0.10829600251970106 1106 26-27 0.08447374039459987 1106 28-29 -0.12822949384425897 1106 30-31 -0.14903523049071765 1106 32-33 -0.1334546345312475 1106 34-35 0.013080283116281066 1106 36-37 0.06817785331177362 1106 38-39 0.013500897904599185 1106 40-41 -2.322835823562741E-4 1106 42-43 -0.10727392358744936 1106 44-45 -0.1604532183913534 1106 46-47 -0.132139972837102 1106 48-49 0.05175207495644685 1106 50-51 0.03526041988322248 1106 52-53 0.03591653903212233 1106 54-55 0.04139551912657424 1106 56-57 0.2976066312691472 1106 58-59 0.23006445933052078 1106 60-61 0.06586116619812543 1106 62-63 0.28672083394814507 1106 64-65 0.308121563883482 1106 66-67 0.19613085451114642 1106 68-69 0.17941348014353053 1106 70-71 0.1880163982320937 1106 72-73 0.08485699656864654 1106 74-75 0.04707767470981139 1106 76-77 -0.14668720767965482 1106 78-79 0.04335201097273256 1106 80-81 0.027774713008543728 1106 82-83 -0.06217782802195515 1106 84-85 -0.03569704651546601 1106 86-87 0.25582865300337687 1106 88-89 0.58509106461457 1106 90-91 0.7005657807629575 1106 92-93 0.7392995297204017 1106 94-95 0.6345831901668753 1106 96-97 0.4560454786700525 1106 98-99 0.5674253086602512 1106 100-101 0.7536389877088219 1107 1 -0.026675701669830687 1107 2 -0.08400507795889922 1107 3 -0.1210925786018393 1107 4 0.050456419123591445 1107 5 0.007082440911197807 1107 6 -0.08308341986411705 1107 7 0.07252955830686147 1107 8 -0.04359861163133161 1107 9 -0.06717274689067665 1107 10-11 -0.005007453112661153 1107 12-13 0.0037156036286063454 1107 14-15 0.039328946925834884 1107 16-17 0.014360766942182579 1107 18-19 0.028331364042784912 1107 20-21 0.09266941501989834 1107 22-23 0.0856879277807252 1107 24-25 0.026531249068590057 1107 26-27 0.06443777841154485 1107 28-29 0.057211373429382206 1107 30-31 -0.02426118210377126 1107 32-33 -0.06913334560810824 1107 34-35 0.10965794863178502 1107 36-37 0.08369597225269843 1107 38-39 -0.04852995519355119 1107 40-41 -0.17526272758916406 1107 42-43 -0.09148585192986758 1107 44-45 -0.07626007970938531 1107 46-47 0.028643627356977674 1107 48-49 -0.15928316398144204 1107 50-51 -0.2545391729281832 1107 52-53 -0.04982377358977175 1107 54-55 0.12802536757251204 1107 56-57 0.08708513494620718 1107 58-59 -7.546271590044284E-4 1107 60-61 0.004248918193638929 1107 62-63 -0.02917141329523787 1107 64-65 -0.22149593571219128 1107 66-67 -0.11530182142191592 1107 68-69 -0.24277116275777644 1107 70-71 -0.07153303046709425 1107 72-73 -0.053208952761110595 1107 74-75 -0.187746701425894 1107 76-77 -0.2120443063658044 1107 78-79 -0.13528652352889026 1107 80-81 -0.08045848808363232 1107 82-83 -0.0027739597303373387 1107 84-85 0.11504122064971867 1107 86-87 0.06348318012232923 1107 88-89 0.3226399415161332 1107 90-91 0.2804551358257008 1107 92-93 0.49650144008182906 1107 94-95 0.5301768958684683 1107 96-97 0.7632623731910826 1107 98-99 0.8108822256337369 1107 100-101 1.1630264447780156 1108 1 0.054139697707455525 1108 2 0.003487823523471434 1108 3 0.07340190903205013 1108 4 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-1.5399502923694826 2316 92-93 -1.3964802638002176 2316 94-95 -1.29343098089916 2316 96-97 -1.3729569613454835 2316 98-99 -1.5547826816298134 2316 100-101 -1.1728911240163242 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 6.0 5 47.0 6 123.0 7 235.0 8 478.0 9 846.0 10 1398.0 11 2188.0 12 3317.0 13 4515.0 14 5429.0 15 7319.0 16 9783.0 17 14268.0 18 13298.0 19 7735.0 20 6571.0 21 6847.0 22 7141.0 23 7663.0 24 8834.0 25 10170.0 26 11822.0 27 13868.0 28 16740.0 29 20288.0 30 24230.0 31 29248.0 32 35999.0 33 45563.0 34 62737.0 35 90341.0 36 133879.0 37 201737.0 38 137082.0 39 7638.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.09077374028754 20.90025082162057 15.764752160322812 20.244223277769084 2 15.265266741381781 22.910223892545062 39.88017493448383 21.944334431589326 3 18.69485898224533 28.277493616913702 32.21699544125454 20.810651959586426 4 12.127021310660387 18.649145129894755 38.719106283653396 30.50472727579146 5 11.773318277957074 39.573660394529504 34.52533432204461 14.12768700546881 6 27.323611942059273 38.65074616804159 18.214863532564273 15.810778357334861 7 24.844530769425226 36.33229546737674 22.04587395616526 16.77729980703277 8 24.83073234855443 34.319411323552956 21.001301896808876 19.848554431083734 9 26.538787255736352 17.42835354292889 22.29340288018336 33.7394563211514 10-11 23.55811768473031 27.64813816116555 27.59125917436991 21.202484979734226 12-13 26.31611655557709 26.14332030032105 28.103380718444804 19.437182425657056 14-15 23.172560895325873 26.789993416721526 28.496273864384463 21.541171823568135 16-17 19.38967529297261 30.201761495991786 29.53794832011132 20.870614890924287 18-19 19.27043065910245 28.4210675997649 32.99019783395936 19.3183039071733 20-21 20.825082369199396 27.13011805549704 32.36688210460677 19.677917470696787 22-23 20.45431564045739 27.05412140925063 32.071532699097524 20.42003025119446 24-25 20.36214084530779 27.601587137738022 32.55839061621138 19.4778814007428 26-27 20.457528249896775 27.002035003749803 33.157868681165894 19.382568065187535 28-29 19.532876054367883 27.130486715596643 33.6020514354571 19.73458579457838 30-31 19.704250334954033 27.839051427030583 32.98101716481423 19.475681073201148 32-33 19.341857457045833 28.110016600237625 32.484063350551516 20.064062592165026 34-35 20.13665287882802 27.312983234950355 32.44347082721238 20.106893059009245 36-37 20.626270533319776 27.17628299172372 32.386266361203106 19.811180113753394 38-39 20.58173655044331 27.06149986542411 32.67344620150988 19.683317382622697 40-41 19.86906555149421 27.403905912590986 33.121306605711396 19.60572193020341 42-43 19.48673301869054 28.100118978585435 32.12000457164286 20.293143431081166 44-45 19.69070954157325 28.365652253371067 31.36770678131154 20.575931423744148 46-47 20.165004160653908 28.161148972476486 30.455512603094686 21.218334263774924 48-49 20.386336392957737 28.139924622427458 30.44048900620843 21.033249978406367 50-51 20.725558878782277 28.678077289510977 30.175425291388 20.420938540318744 52-53 20.26482434926226 28.867244444818958 30.405347046084618 20.462584159834165 54-55 19.671176257446934 28.52676050997278 30.510573171656567 21.291490060923714 56-57 20.373263084273592 29.279090441802264 29.233692583822773 21.11395389010137 58-59 20.154206308870812 28.402979847321006 29.988176462998123 21.454637380810055 60-61 20.24522591045952 28.432396448379688 30.147271425930576 21.175106215230212 62-63 20.802532205567903 28.259266671582207 30.135563584271672 20.802637538578214 64-65 20.476480892206155 28.33126355494182 30.28965310935487 20.902602443497155 66-67 19.852700689254178 28.695715374418224 29.94961339724309 21.501970539084507 68-69 19.64902671273279 28.99922052751327 30.005424707171148 21.34632805258279 70-71 20.046809348808647 28.87896665518553 29.314144028279422 21.76007996772641 72-73 19.904064108938005 28.411738558897138 29.150428067041368 22.53376926512349 74-75 19.804209887674535 29.062002308865537 28.947401683618008 22.18638611984192 76-77 20.030058002139167 28.278401833633016 29.67191864624072 22.019621517987094 78-79 19.599715805700846 28.004725335900705 29.992084061682174 22.40347479671628 80-81 19.559755019194096 29.133629772256754 29.229323753753107 22.07729145479605 82-83 19.488215516587598 28.695975495689837 29.05742039048478 22.75838859723779 84-85 19.376183664741905 27.973302632659987 29.100351597144247 23.550162105453865 86-87 19.46864120682275 28.183218517323617 29.291314258482572 23.056826017371062 88-89 19.959221656326594 27.407300423919136 29.44738149389039 23.18609642586388 90-91 19.351164534445324 27.458646680154107 29.777721609482526 23.412467175918042 92-93 19.220936944376565 28.08505304492176 29.43366435499229 23.260345655709386 94-95 18.98971375363463 27.721936917412464 29.309297858748053 23.97905147020486 96-97 18.838794418275427 27.355790702181626 29.006071305183152 24.799343574359796 98-99 19.05008932107556 27.356054030824197 28.766389574713706 24.827467073386533 100-101 19.335714184144294 26.719687206552063 29.757299628547244 24.1872989807564 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3257.0 1 2160.0 2 1080.0 3 1055.0 4 917.5 5 949.5 6 1488.5 7 2774.5 8 4135.0 9 4497.5 10 3703.5 11 2883.0 12 2714.0 13 2748.0 14 2840.0 15 2847.5 16 2884.5 17 2928.5 18 3018.0 19 3191.0 20 3357.0 21 3642.0 22 4191.0 23 4781.5 24 5281.0 25 6294.0 26 7640.5 27 8858.0 28 9554.0 29 10675.0 30 12754.5 31 14804.0 32 17363.0 33 20459.0 34 23596.5 35 26213.0 36 29843.5 37 32767.0 38 32700.5 39 31458.5 40 29711.0 41 28524.0 42 27694.0 43 28366.0 44 29766.0 45 30457.5 46 30929.5 47 33552.5 48 49089.0 49 48631.0 50 32520.0 51 27842.5 52 23875.5 53 21448.5 54 20349.5 55 18964.5 56 17338.5 57 15470.5 58 14286.5 59 13184.0 60 11580.5 61 9747.0 62 7924.5 63 5918.0 64 4114.0 65 3067.5 66 2226.5 67 1766.5 68 1331.0 69 818.0 70 555.0 71 407.5 72 328.0 73 274.5 74 215.0 75 187.5 76 127.0 77 58.5 78 31.5 79 13.5 80 4.5 81 3.0 82 3.0 83 2.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3888837393509897 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.479831132608092E-4 16-17 0.002159294869094065 18-19 2.1066291405795758E-4 20-21 0.0 22-23 0.0 24-25 2.1066291405795758E-4 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.012376446200905007 36-37 0.02375224356003472 38-39 0.011112468716557264 40-41 0.005687898679564854 42-43 0.005635232951050366 44-45 0.0018433004980071288 46-47 0.001474640398405703 48-49 0.0031599437108693635 50-51 5.793230136593834E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 6.846544706883621E-4 60-61 0.0015273061269201926 62-63 0.001474640398405703 64-65 7.373201992028514E-4 66-67 0.0025806206972099803 68-69 0.002527954968695491 70-71 1.0533145702897879E-4 72-73 0.0 74-75 0.0 76-77 0.09316567374213174 78-79 0.0035286038104707894 80-81 3.686600996014257E-4 82-83 0.0 84-85 2.1066291405795758E-4 86-87 0.0958516258963707 88-89 0.4502393130703698 90-91 0.4016288456514961 92-93 0.0 94-95 0.0010006488417752986 96-97 0.0 98-99 0.0 100-101 3.686600996014257E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 949384.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.227252651286925 #Duplication Level Percentage of deduplicated Percentage of total 1 71.00728020590927 28.564278009238375 2 14.03486174043331 11.291678583165828 3 5.557543517113035 6.706941215502833 4 2.714752491760784 4.368281374870872 5 1.5437631486469034 3.10506751071826 6 1.0303124800841124 2.486798426767057 7 0.677020419795064 1.9064270034023463 8 0.5142145507529352 1.6548350920085064 9 0.3772271970355034 1.3657332385875591 >10 2.2158262049543014 16.902319504104117 >50 0.21220913366350636 5.917252338333458 >100 0.10322868043803246 7.215968038809637 >500 0.006272122353741914 1.7560793440629725 >1k 0.0044427533339005225 3.842369728577441 >5k 0.0010453537256236523 2.9159705918507344 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8927 0.9402939168976937 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7603 0.8008350677913257 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 6069 0.6392566127088722 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 5138 0.541193026214893 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4982 0.5247613189183723 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3253 0.342643229715268 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3172 0.3341113816959207 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3074 0.3237888989070808 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2561 0.26975386145121466 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2356 0.24816091276027405 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2163 0.2278319415536811 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2049 0.21582415545237754 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1849 0.1947578640465818 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1660 0.1748502186681048 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1550 0.16326375839491714 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1487 0.15662787660209146 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1428 0.15041332063738172 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1372 0.1445147590437589 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1355 0.14272412427426626 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1162 0.12239515306767335 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1076 0.11333664776318117 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 971 0.1022768447751384 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 957 0.10080220437673271 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 957 0.10080220437673271 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.2665728514489394E-5 0.0 12-13 0.0 0.0 0.0 1.0533145702897879E-4 0.0 14-15 0.0 0.0 0.0 3.1599437108693635E-4 0.0 16-17 0.0 0.0 0.0 3.1599437108693635E-4 0.0 18-19 0.0 0.0 0.0 4.739915566304045E-4 0.0 20-21 0.0 0.0 0.0 8.953173847463197E-4 0.0 22-23 0.0 0.0 0.0 0.002422623511666512 0.0 24-25 0.0 0.0 0.0 0.004055261095615683 0.0 26-27 0.0 0.0 0.0 0.00700454189242709 0.0 28-29 0.0 0.0 0.0 0.017169027495723542 0.0 30-31 0.0 0.0 0.0 0.047399155663040454 0.0 32-33 0.0 0.0 0.0 0.09622028599597213 0.0 34-35 0.0 0.0 0.0 0.17490288439661927 0.0 36-37 0.0 0.0 0.0 0.29060948994295244 0.0 38-39 0.0 0.0 0.0 0.4508713018125437 0.0 40-41 0.0 0.0 0.0 0.6313567534316988 0.0 42-43 0.0 0.0 0.0 0.802151711004188 0.0 44-45 0.0 0.0 0.0 1.006863397740008 0.0 46-47 0.0 0.0 0.0 1.2516010381468405 0.0 48-49 0.0 0.0 0.0 1.514455689162657 0.0 50-51 0.0 0.0 0.0 1.7835775618716978 0.0 52-53 0.0 0.0 0.0 2.0369523817549062 0.0 54-55 0.0 0.0 0.0 2.314658768211809 0.0 56-57 0.0 0.0 0.0 2.6281778500585644 0.0 58-59 0.0 0.0 0.0 3.0001032248278885 0.0 60-61 0.0 0.0 0.0 3.441284032593766 0.0 62-63 0.0 0.0 0.0 3.856763964844573 0.0 64-65 0.0 0.0 0.0 4.271401245439147 0.0 66-67 0.0 0.0 0.0 4.740600220774734 0.0 68-69 0.0 0.0 0.0 5.25414373951952 0.0 70-71 0.0 0.0 0.0 5.84621185947941 0.0 72-73 0.0 0.0 0.0 6.460820911243501 0.0 74-75 0.0 0.0 0.0 7.083013827913678 0.0 76-77 0.0 0.0 0.0 7.702889452529218 0.0 78-79 0.0 0.0 0.0 8.400236363789574 1.0533145702897879E-4 80-81 0.0 0.0 0.0 9.178899159876298 1.0533145702897879E-4 82-83 0.0 0.0 0.0 9.982367514093347 1.0533145702897879E-4 84-85 0.0 0.0 0.0 10.825335164696266 1.0533145702897879E-4 86-87 0.0 0.0 0.0 11.711488712681064 1.0533145702897879E-4 88-89 0.0 0.0 0.0 12.59911690106427 1.0533145702897879E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGTCCG 20 0.0020827402 71.174194 5 GGACGTG 125 5.7098077E-9 34.163616 6 TAGGACG 115 3.8957896E-6 28.882284 4 CTTTGCG 345 0.0 24.756245 2 GAGTACT 6565 0.0 24.649504 12-13 GTACTTT 7040 0.0 22.919556 14-15 GTACATG 7020 0.0 22.86032 1 TTTGCGT 385 0.0 22.184166 3 TACATGG 7305 0.0 21.824806 2 GTATAGG 505 0.0 21.688322 1 AACGGCG 55 0.0028941112 21.570211 58-59 AGTACTT 6895 0.0 21.542624 12-13 ACATGGG 7235 0.0 20.986633 3 CATGGGG 1870 0.0 20.806717 4 CTGGTCG 255 1.2050805E-8 20.469473 9 ACTTTTT 8005 0.0 20.243938 16-17 GTCGGGA 335 3.45608E-11 19.829628 2 ACGCAGT 145 6.189164E-4 19.634262 7 CCTATAC 390 0.0 19.466448 3 GTGTAAC 125 0.005943119 19.048004 1 >>END_MODULE