FastQCFastQC Report
Fri 27 May 2016
SRR938980_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938980_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences956882
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86900.908157954690338No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75980.7940373003149814No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA60370.6309032879707216No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA51440.5375793462516799No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50070.5232620114078852No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT33110.34601967640733133No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31130.3253274698447666No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30960.3235508662510111No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25010.2613697404695668No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG22640.23660179625073938No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA20880.2182087237506819No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT20540.2146555165631708No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC18150.18967856015684276No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16710.1746296826567957No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15970.16689623171927154No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG15290.15978981734424935No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT14580.15236988468797616No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT14380.15027976281296962No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG13770.1439048910941997No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA11300.11809188593786904No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG10290.10753677046908607No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT10240.10701424000033441No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAAT650.005871736429.2020362
CTTTGCG2950.027.3459762
TTTGCGT3200.025.2095723
GAGTACT69700.023.86531412-13
GTATAGT2955.456968E-1222.608821
GTCGGGA4000.022.5403212
CTTACTA3650.022.1026979
AGTACTT72200.021.9543812-13
GTACTTT75250.021.91594514-15
CTCCCGC2205.6958015E-821.5696877
CATGGGG15850.021.5560784
CGTAACC804.7838097E-520.89622388-89
CGTCACT1856.3854786E-620.6010881
GTACATG68500.020.0296731
ACTTTTT84300.019.78680216-17
ATGGGGA8450.019.6552185
ATGGGAT18900.019.332835
CGGGAGT4950.019.1730544
TACATGG72050.019.0340142
ACATGGG70700.018.793393
Produced by FastQC (version 0.11.5)