##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938980_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 956882 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1354503481098 33.0 31.0 34.0 30.0 34.0 2 32.34871175338234 34.0 31.0 34.0 30.0 34.0 3 32.41329233907629 34.0 31.0 34.0 30.0 34.0 4 36.009009470342214 37.0 35.0 37.0 35.0 37.0 5 35.935630516615426 37.0 35.0 37.0 35.0 37.0 6 35.997460501921864 37.0 35.0 37.0 35.0 37.0 7 35.945399746259206 37.0 35.0 37.0 35.0 37.0 8 35.92270729306226 37.0 35.0 37.0 35.0 37.0 9 37.578483031345556 39.0 37.0 39.0 35.0 39.0 10-11 37.504029754975015 39.0 37.0 39.0 34.5 39.0 12-13 37.43640124905683 39.0 37.0 39.0 34.0 39.0 14-15 38.600989463695626 40.0 38.0 41.0 34.0 41.0 16-17 38.590440096062004 40.0 38.0 41.0 34.0 41.0 18-19 38.75219201531641 40.0 38.0 41.0 34.0 41.0 20-21 38.77299552086882 40.0 38.0 41.0 34.0 41.0 22-23 38.705979943190485 40.0 38.0 41.0 34.0 41.0 24-25 38.628201282916805 40.0 38.0 41.0 34.0 41.0 26-27 38.42606455132399 40.0 38.0 41.0 34.0 41.0 28-29 38.21222574988347 40.0 38.0 41.0 33.5 41.0 30-31 37.98150712418041 40.0 37.0 41.0 33.0 41.0 32-33 37.7334002520687 40.0 36.5 41.0 33.0 41.0 34-35 37.64466203774342 40.0 36.0 41.0 33.0 41.0 36-37 37.4735845172132 40.0 36.0 41.0 33.0 41.0 38-39 37.2839488045548 40.0 36.0 41.0 32.5 41.0 40-41 37.06195539261894 40.0 35.5 41.0 31.5 41.0 42-43 36.831079485244786 40.0 35.0 41.0 31.0 41.0 44-45 36.47071791506163 40.0 35.0 41.0 30.5 41.0 46-47 35.98663419313981 39.0 35.0 41.0 29.5 41.0 48-49 35.54904000702281 39.0 35.0 41.0 27.0 41.0 50-51 35.23570304384449 39.0 35.0 41.0 26.0 41.0 52-53 34.968483052246775 39.0 35.0 40.0 25.0 41.0 54-55 34.72686444096556 38.0 35.0 40.0 24.0 41.0 56-57 34.47872830714759 38.0 34.0 40.0 23.5 41.0 58-59 34.19606754019827 38.0 34.0 40.0 23.0 41.0 60-61 33.78562560482902 37.5 33.5 40.0 22.0 41.0 62-63 33.46401332661708 37.0 33.0 40.0 21.0 41.0 64-65 33.19222171594826 36.5 33.0 39.5 20.0 41.0 66-67 32.74595561417186 36.0 33.0 39.0 17.5 40.5 68-69 32.340289084756535 35.5 32.0 39.0 14.5 40.0 70-71 31.88726196124496 35.0 32.0 38.0 10.5 40.0 72-73 31.376737675073834 35.0 31.0 37.0 7.5 39.5 74-75 30.907934834180182 35.0 31.0 37.0 4.5 39.0 76-77 28.91298352356926 32.5 28.0 35.0 2.0 37.0 78-79 29.85833833220815 34.0 30.0 36.0 2.0 38.0 80-81 29.868378755165214 34.0 30.0 35.5 2.0 37.0 82-83 29.55160563162438 34.0 30.0 35.0 2.0 37.0 84-85 29.16575398011458 34.0 29.5 35.0 2.0 36.0 86-87 28.61059096105894 34.0 29.0 35.0 2.0 36.0 88-89 28.036684774089174 34.0 28.5 35.0 2.0 35.5 90-91 27.576078346128362 33.0 27.0 35.0 2.0 35.0 92-93 27.254377237736733 33.0 26.0 35.0 2.0 35.0 94-95 26.90406758618095 33.0 26.0 35.0 2.0 35.0 96-97 26.324221795372885 33.0 25.0 35.0 2.0 35.0 98-99 25.526782821706334 33.0 22.0 34.5 2.0 35.0 100-101 21.592842691157323 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.03767267992392931 1101 2 -0.003882325513153262 1101 3 0.09193248912724528 1101 4 -0.04220682456062974 1101 5 -0.06265386796909667 1101 6 0.01063535581378261 1101 7 0.12115322029522702 1101 8 0.1820906542162959 1101 9 0.10131979041850059 1101 10-11 0.20287745832710158 1101 12-13 0.06502989595723108 1101 14-15 0.1631429612113422 1101 16-17 0.20575317008315608 1101 18-19 0.20457510115202382 1101 20-21 0.1566046218082704 1101 22-23 0.1695791662295889 1101 24-25 0.1377174331717299 1101 26-27 0.10838461390218157 1101 28-29 0.12015709744542136 1101 30-31 0.13245597565418166 1101 32-33 0.12490588917665946 1101 34-35 0.25178902146346616 1101 36-37 0.11220878842075166 1101 38-39 0.16095885944281463 1101 40-41 0.31965733264580365 1101 42-43 0.10626718346902209 1101 44-45 0.38271949316592213 1101 46-47 0.38855380466815603 1101 48-49 0.32342276355556976 1101 50-51 0.357754058225197 1101 52-53 0.561905986023028 1101 54-55 0.5981254385019596 1101 56-57 0.3903091459779162 1101 58-59 0.2448723587487862 1101 60-61 0.3680027389042593 1101 62-63 0.2341451190978745 1101 64-65 0.26848058225868954 1101 66-67 0.28398091687250826 1101 68-69 0.1806263693268093 1101 70-71 0.17401129605339705 1101 72-73 0.03353843658966582 1101 74-75 -0.07419619929686405 1101 76-77 -0.3921835269434588 1101 78-79 -0.10783170333943204 1101 80-81 0.10293756256372788 1101 82-83 0.302642930994363 1101 84-85 0.15200758020785443 1101 86-87 0.2964746146650512 1101 88-89 0.33118997093822244 1101 90-91 0.11325859993829823 1101 92-93 0.25987862572812404 1101 94-95 0.03907356550313423 1101 96-97 0.19236972807566133 1101 98-99 0.07170974837384136 1101 100-101 0.5731915095857545 1102 1 0.10461215534792245 1102 2 0.11021280402281519 1102 3 0.0796996121117246 1102 4 0.017350119253627838 1102 5 0.020129357094809563 1102 6 -0.033834880239382414 1102 7 0.09670406634607076 1102 8 -0.03124806492138532 1102 9 -0.04647488870191552 1102 10-11 0.007881138011917699 1102 12-13 0.15642441350352243 1102 14-15 0.10830459067538101 1102 16-17 0.14022365513755375 1102 18-19 0.09612745781031862 1102 20-21 0.18488424465440545 1102 22-23 0.1744353818535842 1102 24-25 0.05684667587598824 1102 26-27 0.1820371022544549 1102 28-29 0.04273016547255537 1102 30-31 0.11951621181801642 1102 32-33 0.2940634903541621 1102 34-35 0.20055840205907316 1102 36-37 0.22271899193774658 1102 38-39 0.12940556211320597 1102 40-41 0.08993571601916273 1102 42-43 0.06912035021130691 1102 44-45 0.03369862132961998 1102 46-47 0.20780035461780244 1102 48-49 0.23339775276551933 1102 50-51 0.28161847428965814 1102 52-53 0.21966555597024495 1102 54-55 0.280370129242435 1102 56-57 0.25958142605450263 1102 58-59 0.2884766238631613 1102 60-61 0.1565149288977281 1102 62-63 0.2780080688217552 1102 64-65 0.24582562049420886 1102 66-67 0.25757848662051686 1102 68-69 0.1089257739372087 1102 70-71 0.0336472285906666 1102 72-73 0.3350941409311403 1102 74-75 0.1316040076508216 1102 76-77 -0.04404868447823418 1102 78-79 0.10556442441479774 1102 80-81 0.3753808732840618 1102 82-83 0.41972400026097034 1102 84-85 0.17343413591219914 1102 86-87 0.2823576283545819 1102 88-89 0.15490955128814932 1102 90-91 0.2980327281573274 1102 92-93 0.07427726009471769 1102 94-95 0.13015060648320187 1102 96-97 0.013695438282500305 1102 98-99 0.04661111622058911 1102 100-101 0.8415719502625905 1103 1 0.025509199180746123 1103 2 -0.028885323559137532 1103 3 -0.09485776757603048 1103 4 0.009915439281520833 1103 5 0.04627748735931192 1103 6 -0.04956399660828481 1103 7 0.049799725984463805 1103 8 0.11069624539568679 1103 9 0.1293137802180908 1103 10-11 0.08993154307657392 1103 12-13 0.043993489097708505 1103 14-15 0.021097658973040723 1103 16-17 0.05341575235306806 1103 18-19 0.10055701411697981 1103 20-21 0.24198897130283115 1103 22-23 0.0967816718906036 1103 24-25 -0.005407871041761325 1103 26-27 -5.954155844847264E-4 1103 28-29 0.0838066717228898 1103 30-31 0.04649459048640381 1103 32-33 0.1085626587644839 1103 34-35 0.0011566101174480536 1103 36-37 -0.0697853001380011 1103 38-39 0.06107102163356615 1103 40-41 -0.08534058635756736 1103 42-43 0.047755201866444 1103 44-45 -0.04369152835651846 1103 46-47 0.0010101566458118327 1103 48-49 -0.12122695886063894 1103 50-51 -0.2871023530079526 1103 52-53 -0.3960642310355027 1103 54-55 -0.11475769413865322 1103 56-57 0.028351880478020064 1103 58-59 -0.04784909103570811 1103 60-61 -0.22437728634987053 1103 62-63 -0.0669031388610577 1103 64-65 -0.0294694597139582 1103 66-67 0.04453862396694319 1103 68-69 -0.11612586509922806 1103 70-71 -0.13306973583875603 1103 72-73 0.0870912974309519 1103 74-75 -0.10764098939837652 1103 76-77 -0.28500514673479316 1103 78-79 -0.06643045191976782 1103 80-81 -0.28964139185891824 1103 82-83 -0.05912880836610057 1103 84-85 -0.2826780482178535 1103 86-87 -0.20887178309452992 1103 88-89 -0.0863574158238194 1103 90-91 -0.1622561821919355 1103 92-93 -0.06106395992781444 1103 94-95 -0.47711306109552964 1103 96-97 -0.5508889010060898 1103 98-99 -0.6001530610421355 1103 100-101 -0.033602415314959444 1104 1 -0.047420777018679416 1104 2 0.008610143782654234 1104 3 -0.03836535277352482 1104 4 -0.06671714179537247 1104 5 -0.01417993202529999 1104 6 -0.0773572678359642 1104 7 -0.07216998596922508 1104 8 0.0019480502177771086 1104 9 0.0333387807125618 1104 10-11 -0.06379446679967771 1104 12-13 -0.008346021836715067 1104 14-15 0.010813304720251438 1104 16-17 -0.0675280954287345 1104 18-19 0.041958808719989804 1104 20-21 0.009185052448948738 1104 22-23 -0.06938656719640335 1104 24-25 -0.04611674582172043 1104 26-27 0.1588433918813834 1104 28-29 0.07533801793886852 1104 30-31 -0.15875142571908896 1104 32-33 -0.09014314369732546 1104 34-35 -0.04154465638966798 1104 36-37 -0.04667105077916034 1104 38-39 0.2543402714209435 1104 40-41 0.13078183167548474 1104 42-43 -0.12353115005243609 1104 44-45 -0.04103918159333375 1104 46-47 -0.18820838011322394 1104 48-49 -0.23135044495280255 1104 50-51 -0.4841256327405432 1104 52-53 -0.35270432808383134 1104 54-55 -0.23106867075342308 1104 56-57 -0.48708486998513223 1104 58-59 -0.5355914646322333 1104 60-61 -0.4172338170038259 1104 62-63 -0.5157457451889798 1104 64-65 -0.6929860113335096 1104 66-67 -0.5274380105920216 1104 68-69 -0.6026076652403134 1104 70-71 -0.5543794927564178 1104 72-73 -0.6147276995165107 1104 74-75 -0.46572889989238675 1104 76-77 -0.6096852234768164 1104 78-79 -0.489147805242947 1104 80-81 -0.5540301917428856 1104 82-83 -0.5043235313629957 1104 84-85 -0.607642940719682 1104 86-87 -0.8880769361500356 1104 88-89 -0.4627210042386771 1104 90-91 -0.6711823872197265 1104 92-93 -0.7652511471743964 1104 94-95 -0.34132253253813616 1104 96-97 -0.4142643633372245 1104 98-99 -0.32087011108927754 1104 100-101 0.5535960392958259 1105 1 0.14323463339440679 1105 2 0.08726229030064303 1105 3 0.1632924258948023 1105 4 0.05659387698892715 1105 5 0.060114721415189365 1105 6 -0.012812052508209604 1105 7 0.013973186044317742 1105 8 0.13574602187114237 1105 9 0.1302754992909385 1105 10-11 0.13488126570577208 1105 12-13 0.14055108580181042 1105 14-15 0.15770246506392738 1105 16-17 0.2627004737926413 1105 18-19 0.19256209820478887 1105 20-21 0.2589098423598415 1105 22-23 0.1284864692158223 1105 24-25 0.16470746011268034 1105 26-27 0.10005887896702603 1105 28-29 0.045031110407045105 1105 30-31 0.1510615038440264 1105 32-33 0.0662576635856027 1105 34-35 0.04237027955156236 1105 36-37 0.22672784210375596 1105 38-39 0.25507718264637447 1105 40-41 0.27521703539130016 1105 42-43 0.23948604574988508 1105 44-45 0.14643018921363193 1105 46-47 0.0852375881796732 1105 48-49 0.13758316423439254 1105 50-51 0.1452279373861316 1105 52-53 -0.03528871980918069 1105 54-55 -0.01203282343225709 1105 56-57 -0.026476124581016336 1105 58-59 0.0012317659962377547 1105 60-61 -0.14924799273753564 1105 62-63 0.056081070413021905 1105 64-65 -0.2122048492227009 1105 66-67 -0.12708941114581762 1105 68-69 -0.016846528284666817 1105 70-71 -0.08380041693120788 1105 72-73 0.014501543245344806 1105 74-75 0.11827596215252001 1105 76-77 -0.08327893613334325 1105 78-79 0.0606247843149994 1105 80-81 0.1493971395159157 1105 82-83 0.11889816484852034 1105 84-85 -0.08938190636429155 1105 86-87 -0.23051734954455583 1105 88-89 -0.09319714183406447 1105 90-91 0.08546912679788221 1105 92-93 0.07880793608631365 1105 94-95 -0.04263267740969212 1105 96-97 0.03983952605728547 1105 98-99 0.3502683171714196 1105 100-101 0.7093130460722676 1106 1 -0.027537395801331854 1106 2 0.00856044139087686 1106 3 -0.06961724691954885 1106 4 0.011764147072504727 1106 5 0.011736834652275263 1106 6 -0.13111464742888757 1106 7 -0.04744607669427836 1106 8 0.025297264071411973 1106 9 -0.2397297964655536 1106 10-11 -0.03214919640654301 1106 12-13 -4.41523164134594E-4 1106 14-15 0.03418009624902396 1106 16-17 -0.0813890014669667 1106 18-19 -0.15005607775247398 1106 20-21 0.06456018428151111 1106 22-23 0.004280257063214776 1106 24-25 -0.10753011212834451 1106 26-27 -0.10346279909703071 1106 28-29 -0.17529538449291238 1106 30-31 -0.2359143889514428 1106 32-33 -0.27051676021307003 1106 34-35 -0.2082755724896117 1106 36-37 -0.3136638136260288 1106 38-39 -0.29461658447849004 1106 40-41 -0.21297688139624427 1106 42-43 -0.4278437417291059 1106 44-45 -0.6169795872133363 1106 46-47 -0.6998387397888237 1106 48-49 -0.6761316938163091 1106 50-51 -0.36228628056878875 1106 52-53 -0.5409897167929003 1106 54-55 -0.44496897502904886 1106 56-57 -0.5830704926591253 1106 58-59 -0.4070077403471615 1106 60-61 -0.44589209487656234 1106 62-63 -0.6076878964539674 1106 64-65 -0.5063323157788489 1106 66-67 -0.3560744679025163 1106 68-69 -0.6997515960933782 1106 70-71 -0.6211285926582271 1106 72-73 -0.6111448844215275 1106 74-75 -0.4933700971139032 1106 76-77 -0.7753560239277775 1106 78-79 -0.6762682183789259 1106 80-81 -0.483255804233945 1106 82-83 -0.3749748751459556 1106 84-85 -0.38522473752175657 1106 86-87 -0.34278047956689406 1106 88-89 -0.2611923526281963 1106 90-91 -0.070264105225327 1106 92-93 -0.0020939542685027845 1106 94-95 0.016501351279490706 1106 96-97 -0.2268651319059174 1106 98-99 -0.2128110501008038 1106 100-101 0.31797275918041734 1107 1 -0.0666154736719804 1107 2 -0.016592099420989825 1107 3 -0.07546560408378866 1107 4 -0.11042967761493117 1107 5 0.013005513706410454 1107 6 0.03965047425838719 1107 7 0.006478539363378388 1107 8 -0.07196275757211623 1107 9 0.07674230871277388 1107 10-11 0.1900204520012707 1107 12-13 0.15554037916369623 1107 14-15 0.09239197255857334 1107 16-17 0.12619083781833496 1107 18-19 -0.025853319354098403 1107 20-21 0.0661903562784687 1107 22-23 0.07977126578970228 1107 24-25 -0.11687712232843239 1107 26-27 0.022435752628695127 1107 28-29 -0.01974405019252856 1107 30-31 0.038641803629893445 1107 32-33 0.10051890555562437 1107 34-35 -0.03311151916363997 1107 36-37 -0.008282864534358225 1107 38-39 0.0061478464579423076 1107 40-41 -0.07917268623610596 1107 42-43 0.006081334829111995 1107 44-45 -0.2400794467606744 1107 46-47 -0.32429131974323866 1107 48-49 -0.24892647173991378 1107 50-51 -0.06355150586868774 1107 52-53 0.012891151711158955 1107 54-55 -0.16353175276844922 1107 56-57 -0.14103715241827786 1107 58-59 -0.2749994973870642 1107 60-61 -0.13747069081033914 1107 62-63 -0.26565340496171785 1107 64-65 -0.4097423224561041 1107 66-67 -0.0735562205676672 1107 68-69 -0.030229825929204424 1107 70-71 -0.07469977453292032 1107 72-73 0.04769212140380219 1107 74-75 0.09900401354438415 1107 76-77 -0.17642668773933678 1107 78-79 -0.11335808506401435 1107 80-81 -0.16537802108365796 1107 82-83 -0.36343588226068846 1107 84-85 -0.45608426182467454 1107 86-87 -0.28779786346319014 1107 88-89 -0.09212647802250018 1107 90-91 0.03247126812550505 1107 92-93 0.007073460711580992 1107 94-95 0.13295618768666984 1107 96-97 0.20525708494379558 1107 98-99 0.13185289961253233 1107 100-101 0.6434672216611332 1108 1 -0.0441899759769413 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3168.0 13 4502.0 14 5330.0 15 7289.0 16 9746.0 17 14069.0 18 13087.0 19 7538.0 20 6286.0 21 6278.0 22 6858.0 23 7005.0 24 8279.0 25 9430.0 26 10684.0 27 13258.0 28 15657.0 29 19001.0 30 22922.0 31 27481.0 32 34113.0 33 43123.0 34 58199.0 35 86283.0 36 127179.0 37 203411.0 38 167688.0 39 13730.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.973233865298454 20.865204024887994 15.788976675375366 20.372585434438186 2 15.25768067536018 22.970648418509285 39.85527996137455 21.91639094475599 3 18.754141053964858 28.26419558524458 32.199059027131874 20.782604333658696 4 12.17213825738179 18.66478834380833 38.66850876074584 30.49456463806405 5 11.746023783621093 39.616377026514385 34.55167174046403 14.085927449400499 6 27.228017665710087 38.63527582293324 18.29410522927592 15.842601282080759 7 24.818211649921306 36.33729132745731 22.021315062881317 16.823181959740072 8 24.760315273983625 34.365470350576146 21.06811498178459 19.806099393655643 9 26.51685369773912 17.50445718489845 22.22019015928819 33.75849895807425 10-11 23.57479814647992 27.55595778789861 27.659523326805186 21.20972073881628 12-13 26.264471481332073 26.14486425703483 28.081518933369004 19.50914532826409 14-15 23.17124468385842 26.73289625013129 28.58355885077808 21.512300215232212 16-17 19.417742579389714 30.19745794064348 29.536599844073645 20.84819963589316 18-19 19.267213721232086 28.45700932821393 32.86868182283709 19.407095127716897 20-21 20.889984344987155 27.1520417355536 32.262546479085195 19.695427440374047 22-23 20.46579411045458 27.08097759180338 32.016225616115676 20.437002681626364 24-25 20.37806124475118 27.615317249148795 32.48493544658589 19.521686059514128 26-27 20.480842987954627 27.111179852897223 33.026329265259456 19.381647893888694 28-29 19.58130678599869 27.137776653756678 33.50366084846407 19.777255711780555 30-31 19.664389130530203 27.852180310633912 32.92898183893102 19.554448719904858 32-33 19.396069734826238 28.03788763922824 32.44151316463263 20.12452946131289 34-35 20.12795980197792 27.416829786500262 32.30384077971455 20.15136963180727 36-37 20.559580445306555 27.245292760215044 32.35255386570571 19.84257292877269 38-39 20.544975759751622 27.055587172134764 32.69023643452781 19.709200633585798 40-41 19.8533745793533 27.357136885228766 33.13744957465042 19.65203896076751 42-43 19.524287266950925 28.166384499623774 32.00124362511495 20.30808460831034 44-45 19.685498539784792 28.390565374219012 31.33743590493892 20.58650018105728 46-47 20.147510659643842 28.165914221218962 30.49363556558816 21.192939553549035 48-49 20.399747303605103 28.20558910500225 30.326598072171052 21.068065519221594 50-51 20.717291773132807 28.692715991710532 30.18946008734665 20.40053214781001 52-53 20.21795790912568 28.97034326071553 30.319464677985376 20.492234152173413 54-55 19.60769457467065 28.543853892120453 30.532291337907914 21.316160195300988 56-57 20.325912703969767 29.36166632876363 29.214521748763172 21.09789921850343 58-59 20.191779633694733 28.414549585397115 29.931407246157953 21.462263534750196 60-61 20.286344176315023 28.47347378992962 30.058927392903108 21.181254640852252 62-63 20.729881009361655 28.346337374932336 30.095142347750297 20.828639267955715 64-65 20.417607223476296 28.476036702616835 30.186752779867902 20.91960329403896 66-67 19.839717161456456 28.78397401530614 29.84691771409103 21.529391109146374 68-69 19.661437105222454 29.056672393684398 29.95930473168713 21.32258576940602 70-71 20.11078690998472 28.84504045430889 29.263848625013324 21.780324010693064 72-73 19.904909905296577 28.4705428673546 29.11513645360661 22.509410773742218 74-75 19.79277486670248 29.128565486653528 28.877385090324616 22.20127455631938 76-77 20.06989567965178 28.316068681922342 29.619026692197426 21.99500894622846 78-79 19.57388685334242 28.075666592118985 29.970414324859284 22.380032229679312 80-81 19.56380201529551 29.114718429231605 29.221210138763194 22.10026941670969 82-83 19.477793500138993 28.785158462589955 28.991714756887475 22.74533328038358 84-85 19.354946586935483 27.9761245378218 29.15573707102861 23.513191804214102 86-87 19.49030680804926 28.2587104768523 29.245821106082996 23.005161609015442 88-89 19.90169359701617 27.48559280094003 29.415771743574158 23.19694185846964 90-91 19.210928779535383 27.560030220239696 29.807411508542565 23.421629491682356 92-93 19.199232507247498 28.151903787509852 29.356702289310487 23.292161415932163 94-95 18.989697789278093 27.718412510633495 29.33146406766979 23.960425632418627 96-97 18.829392222005083 27.368873180412574 29.04499941998936 24.756735177592986 98-99 19.0565816892783 27.350864578913598 28.773819551418043 24.81873418039006 100-101 19.292033918497786 26.912409262584102 29.665413290248953 24.13014352866916 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3433.0 1 2209.0 2 1047.0 3 1092.0 4 951.0 5 970.5 6 1567.5 7 2843.0 8 4136.0 9 4514.0 10 3673.0 11 2798.0 12 2677.0 13 2684.0 14 2760.0 15 2849.0 16 2904.5 17 3011.0 18 3102.5 19 3214.0 20 3387.0 21 3734.5 22 4269.0 23 4860.5 24 5436.5 25 6306.5 26 7586.0 27 8896.5 28 9613.0 29 10774.5 30 12948.0 31 14951.0 32 17518.0 33 20800.5 34 23846.5 35 26305.5 36 29785.0 37 32749.5 38 33075.0 39 31656.5 40 29756.0 41 28814.0 42 27951.0 43 28616.5 44 30185.5 45 30800.5 46 31020.0 47 33473.0 48 49475.0 49 49009.5 50 32469.0 51 28173.0 52 24527.5 53 21988.0 54 20831.0 55 19283.5 56 17485.0 57 15759.0 58 14437.5 59 13118.0 60 11479.0 61 9596.0 62 7807.5 63 5947.0 64 4186.5 65 3127.5 66 2256.5 67 1780.5 68 1318.5 69 798.5 70 542.5 71 390.0 72 320.5 73 285.5 74 216.0 75 172.5 76 131.5 77 62.5 78 33.0 79 17.0 80 8.0 81 7.0 82 3.5 83 1.0 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3983772293762449 2 0.0 3 0.0 4 0.0 5 5.225304687516329E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 8.88301796877776E-4 16-17 0.0020901218750065315 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.001985615781256205 36-37 0.006061353437518942 38-39 0.011025392890659454 40-41 0.0022991340625071847 42-43 2.0901218750065314E-4 44-45 2.6126523437581644E-4 46-47 2.0901218750065314E-4 48-49 2.6126523437581644E-4 50-51 5.225304687516329E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.6126523437581644E-4 60-61 0.0027694114843836545 62-63 0.0 64-65 2.0901218750065314E-4 66-67 0.0011495670312535924 68-69 0.0014630853125045722 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.12248114187538275 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.1427553240629461 88-89 0.4333345177357292 90-91 0.33614385054792545 92-93 0.0 94-95 0.0 96-97 3.1351828125097974E-4 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 956882.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.357675654215406 #Duplication Level Percentage of deduplicated Percentage of total 1 71.26787416860279 28.762057502619076 2 13.763171535893681 11.108992256378539 3 5.501816093360188 6.66121528214919 4 2.753215215199414 4.444534666450752 5 1.5962485270687228 3.2210440159479297 6 1.0176059000171955 2.4640925314025957 7 0.6939564494556543 1.9604528513697552 8 0.4913769136126177 1.5864664082837958 9 0.3791546229576302 1.3771619366508336 >10 2.209142552852886 16.87498416997663 >50 0.21469467643185086 6.0103835658054505 >100 0.10036207797754199 7.1434926912788566 >500 0.005690633284928934 1.6027187382881323 >1k 0.004397307538354177 3.3921934084981995 >5k 0.0012933257465747579 3.39020997490037 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8690 0.908157954690338 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7598 0.7940373003149814 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 6037 0.6309032879707216 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 5144 0.5375793462516799 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5007 0.5232620114078852 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3311 0.34601967640733133 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3113 0.3253274698447666 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3096 0.3235508662510111 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2501 0.2613697404695668 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2264 0.23660179625073938 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2088 0.2182087237506819 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2054 0.2146555165631708 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1815 0.18967856015684276 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1671 0.1746296826567957 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1597 0.16689623171927154 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1529 0.15978981734424935 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1458 0.15236988468797616 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1438 0.15027976281296962 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1377 0.1439048910941997 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1130 0.11809188593786904 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1029 0.10753677046908607 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1024 0.10701424000033441 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.0450609375032659E-4 0.0 18-19 0.0 0.0 0.0 2.0901218750065317E-4 0.0 20-21 0.0 0.0 0.0 6.792896093771228E-4 0.0 22-23 0.0 0.0 0.0 0.0013585792187542456 0.0 24-25 0.0 0.0 0.0 0.00381447242188692 0.0 26-27 0.0 0.0 0.0 0.006897402187521554 0.0 28-29 0.0 0.0 0.0 0.01781828898443068 0.0 30-31 0.0 0.0 0.0 0.04890885187515284 0.0 32-33 0.0 0.0 0.0 0.10142316398469195 0.0 34-35 0.0 0.0 0.0 0.18215412140681925 0.0 36-37 0.0 0.0 0.0 0.30667313211033337 0.0 38-39 0.0 0.0 0.0 0.4697026383608428 0.0 40-41 0.0 0.0 0.0 0.6504981805489078 0.0 42-43 0.0 0.0 0.0 0.8295171191432172 0.0 44-45 0.0 0.0 0.0 1.0410897059407533 0.0 46-47 0.0 0.0 0.0 1.299533275785311 0.0 48-49 0.0 0.0 0.0 1.5739662779736685 0.0 50-51 0.0 0.0 0.0 1.8618283132089433 0.0 52-53 0.0 0.0 0.0 2.1310882637566597 0.0 54-55 0.0 0.0 0.0 2.4241233506325752 0.0 56-57 0.0 0.0 0.0 2.758020320164869 0.0 58-59 0.0 0.0 0.0 3.132622413212914 0.0 60-61 0.0 0.0 0.0 3.579594976183061 0.0 62-63 0.0 0.0 0.0 4.0004410157156265 0.0 64-65 0.0 0.0 0.0 4.42076452477944 0.0 66-67 0.0 0.0 0.0 4.886548184624646 0.0 68-69 0.0 0.0 0.0 5.407093037595022 0.0 70-71 0.0 0.0 0.0 6.010302210721907 0.0 72-73 0.0 0.0 0.0 6.619834002520687 0.0 74-75 0.0 0.0 0.0 7.244101153538263 0.0 76-77 0.0 0.0 0.0 7.871503487368349 0.0 78-79 0.0 0.0 0.0 8.57477724526117 0.0 80-81 0.0 0.0 0.0 9.361655878154256 0.0 82-83 0.0 0.0 0.0 10.14984083721922 0.0 84-85 0.0 0.0 0.0 10.991114891909348 0.0 86-87 0.0 0.0 0.0 11.850520753865158 0.0 88-89 0.0 0.0 0.0 12.727065615196022 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGTAAT 65 0.0058717364 29.202036 2 CTTTGCG 295 0.0 27.345976 2 TTTGCGT 320 0.0 25.209572 3 GAGTACT 6970 0.0 23.865314 12-13 GTATAGT 295 5.456968E-12 22.60882 1 GTCGGGA 400 0.0 22.540321 2 CTTACTA 365 0.0 22.102697 9 AGTACTT 7220 0.0 21.95438 12-13 GTACTTT 7525 0.0 21.915945 14-15 CTCCCGC 220 5.6958015E-8 21.569687 7 CATGGGG 1585 0.0 21.556078 4 CGTAACC 80 4.7838097E-5 20.896223 88-89 CGTCACT 185 6.3854786E-6 20.601088 1 GTACATG 6850 0.0 20.029673 1 ACTTTTT 8430 0.0 19.786802 16-17 ATGGGGA 845 0.0 19.655218 5 ATGGGAT 1890 0.0 19.33283 5 CGGGAGT 495 0.0 19.173054 4 TACATGG 7205 0.0 19.034014 2 ACATGGG 7070 0.0 18.79339 3 >>END_MODULE