FastQCFastQC Report
Fri 27 May 2016
SRR938979_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938979_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2045521
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112630.5506176665993652No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91440.447025476638959No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT76730.37511225746399085No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57810.28261748473860693No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT43700.2136375035993275No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT33170.16215917607299069No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC27460.13424452743335316No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA24820.12133828007632286No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT21950.10730762480561187No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA21070.10300554235326843No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT86650.023.76635212-13
GTACATG100050.023.0567991
TACATGG99050.022.713992
ACATGGG100550.021.5743483
GTACTTT98900.020.84754614-15
AGTACTT91000.020.60134712-13
TACATCG1200.004844043419.7352685
CATGGGA64100.019.1379784
GTATCAA228700.019.052691
GTATAGG5800.018.8226031
ACTTTTT116350.018.3945116-17
CCGACGC2850.018.35880994-95
GGTATCA183450.018.0858651
ATGGGAT26300.017.469095
AGAGTAC166250.017.29858210-11
CAACGCA253350.017.012765
CATGGGG36250.016.9859434
TCAACGC255350.016.9166074
ATCAACG255600.016.881533
AACGCAG255600.016.8724486
Produced by FastQC (version 0.11.5)