##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938979_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2045521 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.78552163483044 33.0 31.0 34.0 30.0 34.0 2 31.86215003414778 33.0 31.0 34.0 30.0 34.0 3 31.89726627103804 34.0 31.0 34.0 30.0 34.0 4 35.4414317917049 37.0 35.0 37.0 33.0 37.0 5 35.493854133005726 37.0 35.0 37.0 33.0 37.0 6 35.58346211063098 37.0 35.0 37.0 33.0 37.0 7 35.51045283817668 37.0 35.0 37.0 33.0 37.0 8 35.50304396777154 37.0 35.0 37.0 33.0 37.0 9 37.10138395059254 39.0 37.0 39.0 33.0 39.0 10-11 36.98856868250191 39.0 37.0 39.0 33.0 39.0 12-13 36.88530232639997 39.0 37.0 39.0 33.0 39.0 14-15 38.06616993910109 40.0 38.0 41.0 33.0 41.0 16-17 37.993249397097365 40.0 38.0 41.0 33.0 41.0 18-19 37.951609149942726 40.0 38.0 41.0 33.0 41.0 20-21 38.068170651877935 40.0 38.0 41.0 33.0 41.0 22-23 37.997331975570035 40.0 38.0 41.0 33.0 41.0 24-25 37.91938679681118 40.0 38.0 41.0 33.0 41.0 26-27 37.79467993728737 40.0 38.0 41.0 32.0 41.0 28-29 37.67006425257917 40.0 37.0 41.0 32.0 41.0 30-31 37.45946509471182 40.0 37.0 41.0 32.0 41.0 32-33 37.23642020785903 40.0 36.5 41.0 31.5 41.0 34-35 36.901367182248435 40.0 36.0 41.0 30.5 41.0 36-37 36.62709671521338 39.5 35.5 41.0 30.0 41.0 38-39 36.38380833049379 39.0 35.0 41.0 30.0 41.0 40-41 36.2414568220028 39.0 35.0 41.0 30.0 41.0 42-43 36.01521030583406 39.0 35.0 41.0 29.0 41.0 44-45 35.88699627136559 39.0 35.0 41.0 28.5 41.0 46-47 35.6244846667426 39.0 35.0 41.0 26.5 41.0 48-49 35.35296117712798 39.0 35.0 41.0 25.5 41.0 50-51 34.242391302753674 38.0 34.0 40.0 23.5 40.5 52-53 34.3538013053887 38.0 34.0 39.5 24.0 40.5 54-55 34.86058515165574 39.0 35.0 40.5 24.0 41.0 56-57 34.78850009361918 39.0 35.0 41.0 23.5 41.0 58-59 34.59542483308653 38.5 34.0 40.5 23.0 41.0 60-61 34.29690577608345 38.0 34.0 40.0 22.5 41.0 62-63 33.94351610176576 38.0 34.0 40.0 22.0 41.0 64-65 33.57436002856974 37.0 33.5 40.0 20.5 41.0 66-67 33.16998627733472 37.0 33.0 39.5 19.0 41.0 68-69 32.73403157435196 36.0 32.5 39.0 17.5 41.0 70-71 32.27864979142233 36.0 32.0 39.0 13.5 40.5 72-73 31.837330929381807 35.0 31.5 38.5 9.5 40.0 74-75 31.367696053963755 35.0 31.0 37.5 7.5 39.5 76-77 30.925607461375364 35.0 31.0 37.0 4.5 39.0 78-79 30.51913840043686 35.0 30.5 36.5 2.0 39.0 80-81 30.0474971902024 34.5 30.0 36.0 2.0 38.5 82-83 29.557131899403622 34.0 29.5 35.5 2.0 37.0 84-85 29.116042074366383 34.0 29.0 35.0 2.0 37.0 86-87 28.65745768437479 34.0 29.0 35.0 2.0 36.0 88-89 28.15727851241811 34.0 28.5 35.0 2.0 36.0 90-91 27.67883194550435 34.0 27.0 35.0 2.0 35.5 92-93 27.155791605170513 34.0 26.0 35.0 2.0 35.0 94-95 26.602478537252857 33.0 25.0 35.0 2.0 35.0 96-97 25.94982134135998 33.0 23.5 35.0 2.0 35.0 98-99 25.106295413246798 32.5 13.5 35.0 2.0 35.0 100-101 21.977106810440958 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2673414438857833 1101 2 0.2934935588638403 1101 3 0.37510727850989056 1101 4 0.3981515130808688 1101 5 0.23089418653979976 1101 6 0.3622275213980757 1101 7 0.4235138079673746 1101 8 0.36849700265448604 1101 9 0.471966457981857 1101 10-11 0.3553195432090135 1101 12-13 0.3419914785912255 1101 14-15 0.6279639939259383 1101 16-17 0.45094828207569293 1101 18-19 0.6565751075927864 1101 20-21 0.5959123585290413 1101 22-23 0.6597605648129701 1101 24-25 0.5958515273471647 1101 26-27 0.6699316369685846 1101 28-29 0.5488592196144353 1101 30-31 0.5422654814190437 1101 32-33 0.5939430115507989 1101 34-35 0.5362720007314294 1101 36-37 0.402584661852444 1101 38-39 0.5612355386756178 1101 40-41 0.45902340315698353 1101 42-43 0.40759359502640535 1101 44-45 0.6903522671932194 1101 46-47 0.6392701049711675 1101 48-49 0.4128037156976987 1101 50-51 0.4384319522672939 1101 52-53 0.35549469199009565 1101 54-55 0.4086530756988225 1101 56-57 0.3342060087303409 1101 58-59 0.2192727192240369 1101 60-61 0.43306366678431374 1101 62-63 0.47414332321431374 1101 64-65 0.32305151822882294 1101 66-67 0.26452073796726694 1101 68-69 0.1849017869514782 1101 70-71 0.04198889637964953 1101 72-73 0.19719800064545367 1101 74-75 -0.09402809784538846 1101 76-77 0.05428400813687162 1101 78-79 -0.03175804686095063 1101 80-81 -0.20078881658589864 1101 82-83 -0.14712113890104206 1101 84-85 -0.09454028256839209 1101 86-87 -0.06070589989716524 1101 88-89 -0.17781100770034186 1101 90-91 -0.2687487843567169 1101 92-93 -0.26094571362918373 1101 94-95 -0.5567437305331886 1101 96-97 -0.7644521250059881 1101 98-99 -0.7879715971365613 1101 100-101 -0.2523435779481993 1102 1 0.27427873424953475 1102 2 0.25416779411429147 1102 3 0.3786504995631752 1102 4 0.15775705875078216 1102 5 0.27885167482445183 1102 6 0.18781190139509363 1102 7 0.24039535720305594 1102 8 0.3262818564684338 1102 9 0.3188795372022426 1102 10-11 0.43161412830701806 1102 12-13 0.4310211944744964 1102 14-15 0.5164305752639322 1102 16-17 0.6496001931257567 1102 18-19 0.5378069622426764 1102 20-21 0.5301581189774609 1102 22-23 0.5368118348574029 1102 24-25 0.5755330710330213 1102 26-27 0.6446781657219276 1102 28-29 0.4783728808734864 1102 30-31 0.4900424267219776 1102 32-33 0.3963221552810481 1102 34-35 0.5421166678628921 1102 36-37 0.435164778884527 1102 38-39 0.5331204351539043 1102 40-41 0.4776248801060987 1102 42-43 0.30058242252027867 1102 44-45 0.4209096437874962 1102 46-47 0.4806576324168219 1102 48-49 0.3760360767697861 1102 50-51 0.2505579889586187 1102 52-53 0.4218568116097998 1102 54-55 0.49191356748333703 1102 56-57 0.5599792564575168 1102 58-59 0.33802872431601116 1102 60-61 0.39781442728476435 1102 62-63 0.46388393792761917 1102 64-65 0.3773245422283793 1102 66-67 0.264063743964563 1102 68-69 0.31670683521767984 1102 70-71 0.358947622970625 1102 72-73 0.1760305934230395 1102 74-75 0.11259475983263201 1102 76-77 0.28644476596647195 1102 78-79 0.2738738188886032 1102 80-81 0.22885318454487802 1102 82-83 0.2241296892379978 1102 84-85 0.10709119469290229 1102 86-87 0.12554126882120542 1102 88-89 0.10959746342491883 1102 90-91 0.13412047893773504 1102 92-93 0.2248709962221156 1102 94-95 0.2573542309440384 1102 96-97 0.1625112148830823 1102 98-99 0.19336622462363806 1102 100-101 0.1555300731824012 1103 1 0.12954847100336053 1103 2 0.2399299454973267 1103 3 0.2859002855928843 1103 4 0.368191516164714 1103 5 0.22906996244369537 1103 6 0.35383417764226266 1103 7 0.1337838939963234 1103 8 0.18526566240335995 1103 9 0.3006128628343987 1103 10-11 0.45824241742565874 1103 12-13 0.37164888733509116 1103 14-15 0.45075963619251525 1103 16-17 0.5227263122035097 1103 18-19 0.5973839049091438 1103 20-21 0.49919601986911033 1103 22-23 0.3537123373594113 1103 24-25 0.46695766101809966 1103 26-27 0.44805770030226455 1103 28-29 0.36616639356263647 1103 30-31 0.43353705894102745 1103 32-33 0.46875758934465495 1103 34-35 0.3449619330664504 1103 36-37 0.1839691998287023 1103 38-39 0.3383163105884748 1103 40-41 0.4542812610298128 1103 42-43 0.23476241893806105 1103 44-45 0.32424028754198275 1103 46-47 0.4137811961029598 1103 48-49 0.455221128545638 1103 50-51 0.46536490811898545 1103 52-53 0.41866403064944535 1103 54-55 0.3575310358603687 1103 56-57 0.2955103724343715 1103 58-59 0.40500157123813096 1103 60-61 0.41330138399421656 1103 62-63 0.35124557193136496 1103 64-65 0.18879323313858976 1103 66-67 0.19800264093385067 1103 68-69 0.03514658194219322 1103 70-71 0.22444811508771068 1103 72-73 0.11205493498671615 1103 74-75 0.04202919565991792 1103 76-77 0.01718029285263256 1103 78-79 -0.06437003781577033 1103 80-81 0.14748306874129824 1103 82-83 0.16575445156615842 1103 84-85 0.10825045568298108 1103 86-87 0.04818801860227495 1103 88-89 0.10980122381786828 1103 90-91 0.2425921855601061 1103 92-93 0.05850493812371127 1103 94-95 0.1841855803483341 1103 96-97 -0.01785348850771129 1103 98-99 -0.08676799761258991 1103 100-101 0.2751425287912248 1104 1 0.22850880608960722 1104 2 0.1624093190517506 1104 3 0.14204132790443325 1104 4 0.14973300495106656 1104 5 0.23068210471726758 1104 6 0.22309329412846068 1104 7 0.26903785530951296 1104 8 0.11130446428591512 1104 9 0.20261912268733795 1104 10-11 0.3339408973400424 1104 12-13 0.25844292781700773 1104 14-15 0.3097885247234089 1104 16-17 0.3172954007274953 1104 18-19 0.3575833514184694 1104 20-21 0.34988917775779527 1104 22-23 0.30019749897638803 1104 24-25 0.30544309880505693 1104 26-27 0.2504050013160395 1104 28-29 0.223129760765147 1104 30-31 0.2402487087114622 1104 32-33 0.29374119175193414 1104 34-35 0.36908653175694184 1104 36-37 0.48925799883448917 1104 38-39 0.6341856994473218 1104 40-41 0.5925420230288196 1104 42-43 0.5948091089257517 1104 44-45 0.6035460940158686 1104 46-47 0.4604999514968995 1104 48-49 0.612020951336568 1104 50-51 0.5920869430314752 1104 52-53 0.505206785214888 1104 54-55 0.6493397191049084 1104 56-57 0.6573810788541792 1104 58-59 0.47359759208896435 1104 60-61 0.5244612403858255 1104 62-63 0.6941474280237898 1104 64-65 0.6087026436307568 1104 66-67 0.6844170915281964 1104 68-69 0.793805517767197 1104 70-71 0.662291169987995 1104 72-73 0.6485907853025843 1104 74-75 0.5875276249431458 1104 76-77 0.49821288091058236 1104 78-79 0.33703713454882234 1104 80-81 0.46180924275140356 1104 82-83 0.5264521547524694 1104 84-85 0.5403645656676765 1104 86-87 0.5219966040366089 1104 88-89 0.4978106999003984 1104 90-91 0.4002727316862469 1104 92-93 0.33799672782026846 1104 94-95 0.2502276042140217 1104 96-97 0.10738916709681234 1104 98-99 0.269714373536857 1104 100-101 0.43454611669958254 1105 1 0.17876737969949374 1105 2 0.1973755484466615 1105 3 0.19449471220945824 1105 4 0.182363434160969 1105 5 0.17959041139448573 1105 6 0.17590667531277404 1105 7 0.146299259854608 1105 8 0.24567859355042998 1105 9 0.17457670324081676 1105 10-11 0.23800869489474508 1105 12-13 0.18354783402835295 1105 14-15 0.3418267144852365 1105 16-17 0.29652940427173036 1105 18-19 0.3865573881760511 1105 20-21 0.2953431026154121 1105 22-23 0.324952578498916 1105 24-25 0.38449863640411763 1105 26-27 0.2919089913743278 1105 28-29 0.40512392614080284 1105 30-31 0.3634120837657946 1105 32-33 0.2136273970727558 1105 34-35 0.3948825929675621 1105 36-37 0.3728635124709001 1105 38-39 0.5609470928470088 1105 40-41 0.5178479646491425 1105 42-43 0.5688972747249252 1105 44-45 0.6918148489616414 1105 46-47 0.5687665052233655 1105 48-49 0.5528067587661027 1105 50-51 0.6908628979148403 1105 52-53 0.558807032643081 1105 54-55 0.6874118968073901 1105 56-57 0.7305044697349956 1105 58-59 0.6784369309429934 1105 60-61 0.728883462112698 1105 62-63 0.6930362302261202 1105 64-65 0.7034315592933993 1105 66-67 0.7274565017128722 1105 68-69 0.6405172026194634 1105 70-71 0.6502443703936933 1105 72-73 0.6072516080570658 1105 74-75 0.6163836596276759 1105 76-77 0.619116383914271 1105 78-79 0.6900822010312169 1105 80-81 0.538694350248921 1105 82-83 0.6357556499543655 1105 84-85 0.6007642123580474 1105 86-87 0.6699560180107298 1105 88-89 0.5550430985049921 1105 90-91 0.5426034328442562 1105 92-93 0.5602399096131236 1105 94-95 0.5911296025867934 1105 96-97 0.6098332171174334 1105 98-99 0.4558858401619119 1105 100-101 0.7179723284847448 1106 1 0.18947559227162714 1106 2 0.2570638695809784 1106 3 0.21642836442459767 1106 4 0.22577482816853234 1106 5 0.311035068626488 1106 6 0.19146233893598463 1106 7 0.26349810393007544 1106 8 0.15570513414443354 1106 9 0.22656978503140834 1106 10-11 0.16156665683507754 1106 12-13 0.3366481041916174 1106 14-15 0.3924621082517632 1106 16-17 0.24379748330043327 1106 18-19 0.42754518880601466 1106 20-21 0.27247516440355923 1106 22-23 0.29286877449863624 1106 24-25 0.25376308506437084 1106 26-27 0.3216770347230806 1106 28-29 0.16789192878798787 1106 30-31 0.32604024745376137 1106 32-33 0.3572768423949597 1106 34-35 0.2149224593607002 1106 36-37 0.24732018618017548 1106 38-39 0.43545966513854495 1106 40-41 0.48078139009840726 1106 42-43 0.38986111957478187 1106 44-45 0.21580670547646008 1106 46-47 0.2154487459662704 1106 48-49 0.32549272925270856 1106 50-51 0.29795625621179767 1106 52-53 0.35355629248241627 1106 54-55 0.3670984841606142 1106 56-57 0.332116463271376 1106 58-59 0.4453851696487092 1106 60-61 0.6139769885018467 1106 62-63 0.5349583432823906 1106 64-65 0.41991606441783347 1106 66-67 0.3141458202999061 1106 68-69 0.28183582242399297 1106 70-71 0.3376325401921747 1106 72-73 0.14860849790752084 1106 74-75 0.25355270169776745 1106 76-77 0.18860030388758986 1106 78-79 0.42592730566332193 1106 80-81 0.4273423921599111 1106 82-83 0.47814424526924526 1106 84-85 0.5804650981193156 1106 86-87 0.6110456518499063 1106 88-89 0.775215588579357 1106 90-91 0.8285992125673651 1106 92-93 0.8316507845435446 1106 94-95 0.9062917560486206 1106 96-97 1.0177357437333505 1106 98-99 0.9788280455208778 1106 100-101 1.2635099829855179 1107 1 -5.581821090316907E-4 1107 2 0.07820084255843796 1107 3 0.02702766990916672 1107 4 -0.024596366140933412 1107 5 -0.02791923120539508 1107 6 0.0021021291441485346 1107 7 -0.10289016278733243 1107 8 -0.13325154416496332 1107 9 0.02083654089941689 1107 10-11 -0.02204674644237059 1107 12-13 0.16363056519723784 1107 14-15 0.164332208967636 1107 16-17 0.09669877041101671 1107 18-19 0.06869087612023606 1107 20-21 0.12467657185497671 1107 22-23 0.0377783265655367 1107 24-25 0.15159431842135263 1107 26-27 0.071425776407267 1107 28-29 0.2414893608419746 1107 30-31 0.23447367083186776 1107 32-33 0.36967857837991147 1107 34-35 0.2818834330992743 1107 36-37 0.3145015486328191 1107 38-39 0.38412301138313154 1107 40-41 0.24695579626341413 1107 42-43 0.24172245534798975 1107 44-45 0.12565684115089226 1107 46-47 0.41311844527611186 1107 48-49 0.5593016231392127 1107 50-51 0.4581889725242121 1107 52-53 0.3781239409373711 1107 54-55 0.5133541026811628 1107 56-57 0.4657380647053415 1107 58-59 0.52743783622563 1107 60-61 0.5644188792598968 1107 62-63 0.5157964175455447 1107 64-65 0.5027723527581358 1107 66-67 0.44115272395739424 1107 68-69 0.6358830802418467 1107 70-71 0.4415998406809294 1107 72-73 0.552985351981345 1107 74-75 0.5475598492757179 1107 76-77 0.4024730016984783 1107 78-79 0.5058502227495936 1107 80-81 0.44409572987234114 1107 82-83 0.6286947869042407 1107 84-85 0.6261397102503636 1107 86-87 0.7823780142246193 1107 88-89 0.7903652251783555 1107 90-91 0.7588460580483094 1107 92-93 0.7901185187248991 1107 94-95 0.6904471991691032 1107 96-97 0.8896132329112767 1107 98-99 0.7739123397759968 1107 100-101 1.0022823039848419 1108 1 0.30018733019974064 1108 2 0.4087015020460605 1108 3 0.3670569151540626 1108 4 0.29733646532048397 1108 5 0.201368055038472 1108 6 0.2943963325287626 1108 7 0.3140847283958976 1108 8 0.3223174128368598 1108 9 0.3171191616554623 1108 10-11 0.4059798765978684 1108 12-13 0.45424731340103364 1108 14-15 0.5600995738566468 1108 16-17 0.4455833361962789 1108 18-19 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-2.037377848250731 2316 94-95 -2.301175368772629 2316 96-97 -2.4049180612089884 2316 98-99 -2.534092761968573 2316 100-101 -2.37763182380694 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5878.0 3 1471.0 4 1530.0 5 1692.0 6 2050.0 7 2684.0 8 3132.0 9 4010.0 10 5079.0 11 6765.0 12 9430.0 13 13754.0 14 12462.0 15 13332.0 16 17055.0 17 23022.0 18 19631.0 19 13326.0 20 12921.0 21 13930.0 22 15082.0 23 17023.0 24 19096.0 25 21834.0 26 24920.0 27 29261.0 28 34486.0 29 40923.0 30 48589.0 31 58747.0 32 72332.0 33 91644.0 34 121731.0 35 171799.0 36 254778.0 37 393527.0 38 380792.0 39 65786.0 40 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.23209425940267 20.146848725998286 14.67579938684698 21.945257627752067 2 14.987586471455735 21.880691164914484 40.15578317768716 22.97593918594262 3 18.917256454636608 26.309999701497066 31.289077581891345 23.483666261974985 4 12.028317122012618 17.636980603182494 39.11016194355759 31.224540331247297 5 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30.903009018786733 20.597390027416996 26-27 20.918461338598917 27.146370708390105 31.404310246333694 20.53085770667729 28-29 20.391742498896644 27.823513063628614 31.254923508855708 20.52982092861903 30-31 20.785904312248103 27.45652586064546 30.961635608613484 20.795934218492945 32-33 20.545806029619 27.589974753670333 30.971921316380467 20.8922979003302 34-35 21.21710663446094 27.455903088561655 30.752436886180558 20.574553390796847 36-37 21.14381351254351 27.10480203775667 31.007398880452282 20.743985569247542 38-39 20.821348720789963 27.589142923381 31.000631648021752 20.588876707807287 40-41 20.881389841861633 27.60980848767236 30.847514975558827 20.66128669490718 42-43 20.579611969310644 27.84014949234645 30.292758301302825 21.28748023704008 44-45 20.854896570155727 27.720750719975474 30.104456626083405 21.319896083785395 46-47 21.216549171664532 27.9617718152984 29.76567072857709 21.05600828445998 48-49 21.1361209802672 27.996659855024543 29.699725978397645 21.16749318631061 50-51 20.784595319824138 28.274168875359003 29.83514462891635 21.106091175900513 52-53 21.0257887990268 28.62370482390911 29.173637243095875 21.176869133968214 54-55 20.64906124217579 28.14865962272827 29.47466702431677 21.72761211077917 56-57 21.04584829450612 28.407862710769272 29.11529154855107 21.430997446173546 58-59 21.311365922685514 28.017607256621567 29.2405489413621 21.430477879330823 60-61 21.08295974306644 28.172956441808893 29.626147923337193 21.11793589178748 62-63 20.89351640235526 28.078273870123695 29.75551143747466 21.272698290046392 64-65 20.7022640913331 28.25545889988436 29.53980910771084 21.502467901071697 66-67 20.825737212719222 28.126194699832435 29.048269558679724 21.99979852876862 68-69 20.70644584469711 28.069878412603945 29.384558565337038 21.839117177361906 70-71 21.026673055422457 27.989777990736304 29.099087145086187 21.884461808755052 72-73 20.898657711030367 27.631207499894966 29.480604706917727 21.98953008215694 74-75 20.5869439431557 28.448071468146086 28.970790392782426 21.99419419591579 76-77 20.774917048794077 27.889045331593575 29.115676770812964 22.22036084879938 78-79 20.659792700432355 27.828806560106685 29.242648061033155 22.268752678427806 80-81 20.747986552785814 28.034538552675787 29.01603880908453 22.201436085453864 82-83 20.660266688674575 27.779652707365578 29.271523536670674 22.288557067289176 84-85 20.63199765375495 27.647963026007655 29.13300939824415 22.58702992199325 86-87 20.535168860491044 27.687603731675246 29.193179113485186 22.58404829434852 88-89 20.740853553689774 27.330446231619902 29.045389562218432 22.883310652471884 90-91 20.641451287899596 27.224963677020536 29.17127101684197 22.962314018237894 92-93 20.79316729350094 27.417922710204053 29.122929948198955 22.665980048096056 94-95 20.630601301006777 27.061678040750138 29.188670552897168 23.119050105345924 96-97 20.3926339732115 26.895397682798173 29.368019784514676 23.34394855947565 98-99 20.480619899820077 27.012805900232355 28.90871597760927 23.5978582223383 100-101 20.621003247144838 26.139479105793445 29.592717788256877 23.646799858804837 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3159.0 1 1960.5 2 907.5 3 1014.5 4 867.0 5 903.5 6 1510.0 7 2733.5 8 4126.0 9 4724.0 10 4194.5 11 3690.0 12 3778.5 13 4023.0 14 4365.5 15 4595.0 16 4748.0 17 4858.0 18 4959.0 19 5316.0 20 5780.5 21 6481.0 22 7566.0 23 8926.5 24 10690.0 25 13059.5 26 16096.5 27 19208.5 28 21611.0 29 24552.0 30 28729.5 31 32945.5 32 37392.0 33 43321.0 34 50014.5 35 54750.5 36 60159.0 37 66169.5 38 68282.0 39 67977.0 40 67649.5 41 67588.0 42 67331.0 43 68273.0 44 69781.0 45 70098.5 46 70121.0 47 75021.0 48 91010.0 49 91159.0 50 74325.5 51 65094.5 52 59483.0 53 56047.5 54 52216.0 55 47469.0 56 42559.5 57 37288.0 58 32707.0 59 28225.0 60 23566.5 61 19542.0 62 16025.0 63 12071.5 64 8665.5 65 6367.0 66 4607.5 67 3559.0 68 2751.0 69 1863.5 70 1214.5 71 869.5 72 653.5 73 475.0 74 354.5 75 274.0 76 197.5 77 120.5 78 76.0 79 63.5 80 41.0 81 26.0 82 21.0 83 16.0 84 15.0 85 13.5 86 7.5 87 7.5 88 10.5 89 8.5 90 5.5 91 3.5 92 4.5 93 4.5 94 1.0 95 1.5 96 2.5 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3203095934972068 2 0.08716605696054942 3 0.09728572818367545 4 0.10232112014494107 5 0.1140540722876959 6 0.057735902002472715 7 0.05954473212448076 8 0.061158013044109547 9 0.06374903997563457 10-11 0.06101135114232511 12-13 0.05964250672567038 14-15 0.05756479645039088 16-17 0.059349182922101514 18-19 0.06700004546518956 20-21 0.06897998113927943 22-23 0.08406171337277887 24-25 0.09278809652895277 26-27 0.08846157042631192 28-29 0.08579721254389468 30-31 0.07985740552162506 32-33 0.07144878981931742 34-35 0.32001626969363794 36-37 0.49598610818466293 38-39 0.4681936777965125 40-41 0.44990982737405283 42-43 0.48063549579789205 44-45 0.49058406146893624 46-47 0.48410649414012374 48-49 0.5025614501146651 50-51 0.49823492401202435 52-53 0.49767272005518404 54-55 0.48945965355525556 56-57 0.4671914881343189 58-59 0.44983649642316065 60-61 0.41155774005742307 62-63 0.4801955100925388 64-65 0.5030258794703159 66-67 0.5128766705401705 68-69 0.5245362917320331 70-71 0.5228985671621069 72-73 0.5109945094672702 74-75 0.5127544522886834 76-77 0.4882374710403853 78-79 0.4682670087474047 80-81 0.4746468014750277 82-83 0.4834465155820937 84-85 0.48535312030529143 86-87 0.48498646555083036 88-89 0.49038851226655705 90-91 0.5039302945313199 92-93 0.5097478833021025 94-95 0.5161765633303202 96-97 0.5081346023824737 98-99 0.5124611284851146 100-101 0.4908529416222078 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2045521.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.13502536651744 #Duplication Level Percentage of deduplicated Percentage of total 1 71.05056980850526 33.489704102294134 2 14.578580961485407 13.74323566854886 3 5.734336533141543 8.108642938493228 4 2.778960857436543 5.23945562031322 5 1.5842778191436357 3.7337487596473102 6 1.014863951802391 2.8701382867061898 7 0.6380319475916623 2.105155642406707 8 0.4430189930398654 1.6705369179826457 9 0.3248417104074869 1.3780280034143826 >10 1.6690187153014802 14.248887036272997 >50 0.11706720094851995 3.8113720059436464 >100 0.06121868641592483 5.003883681137706 >500 0.002397894798938636 0.7864562670070009 >1k 0.002397894798938635 2.146783834070722 >5k 3.1276888681808283E-4 1.110493546727331 >10k+ 1.0425629560602762E-4 0.5534776890339821 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11263 0.5506176665993652 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9144 0.447025476638959 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7673 0.37511225746399085 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5781 0.28261748473860693 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4370 0.2136375035993275 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3317 0.16215917607299069 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2746 0.13424452743335316 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2482 0.12133828007632286 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2195 0.10730762480561187 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2107 0.10300554235326843 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.8887300594811786E-5 0.0 10-11 0.0 0.0 0.0 1.2221825148702947E-4 0.0 12-13 0.0 0.0 0.0 1.9554920237924714E-4 0.0 14-15 0.0 0.0 0.0 2.1999285267665304E-4 0.0 16-17 0.0 0.0 0.0 3.1776745386627664E-4 0.0 18-19 0.0 0.0 0.0 3.422111041636825E-4 0.0 20-21 0.0 0.0 0.0 4.155420550559002E-4 0.0 22-23 0.0 0.0 0.0 6.355349077325533E-4 0.0 24-25 0.0 0.0 0.0 9.28858711301424E-4 0.0 26-27 0.0 0.0 0.0 0.0023465904285509655 0.0 28-29 0.0 0.0 0.0 0.007821968095169886 0.0 30-31 0.0 0.0 0.0 0.01977491309060137 0.0 32-33 0.0 0.0 0.0 0.045538520504067176 0.0 34-35 0.0 0.0 0.0 0.08814380297244565 0.0 36-37 0.0 0.0 0.0 0.15768598806856543 0.0 38-39 0.0 0.0 0.0 0.2587360383980414 0.0 40-41 0.0 0.0 0.0 0.36897690123934196 0.0 42-43 0.0 0.0 0.0 0.4821265584660338 0.0 44-45 0.0 0.0 0.0 0.6249752508040739 0.0 46-47 0.0 0.0 0.0 0.7982074004617894 0.0 48-49 0.0 0.0 0.0 0.9805081443798427 0.0 50-51 0.0 0.0 0.0 1.1735640944287544 0.0 52-53 0.0 0.0 0.0 1.3672066920848038 0.0 54-55 0.0 0.0 0.0 1.5838996519713069 0.0 56-57 0.0 0.0 0.0 1.8481355116862648 0.0 58-59 0.0 0.0 0.0 2.129873025014165 0.0 60-61 0.0 0.0 0.0 2.444805015445943 0.0 62-63 0.0 0.0 0.0 2.759908111429802 0.0 64-65 0.0 0.0 0.0 3.0911440166099493 0.0 66-67 0.0 0.0 0.0 3.4742493477211918 0.0 68-69 0.0 0.0 0.0 3.8950956748916292 0.0 70-71 0.0 0.0 0.0 4.355051842537916 0.0 72-73 0.0 0.0 0.0 4.814519137178254 0.0 74-75 0.0 0.0 0.0 5.307498676376336 0.0 76-77 0.0 4.8887300594811786E-5 0.0 5.83372157997889 0.0 78-79 0.0 4.8887300594811786E-5 0.0 6.443981753303926 0.0 80-81 0.0 4.8887300594811786E-5 0.0 7.096651659894961 0.0 82-83 0.0 4.8887300594811786E-5 0.0 7.741719591243502 0.0 84-85 0.0 4.8887300594811786E-5 0.0 8.41225780620194 0.0 86-87 0.0 4.8887300594811786E-5 0.0 9.127747894057308 0.0 88-89 0.0 9.777460118962357E-5 0.0 9.894642978488122 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACT 8665 0.0 23.766352 12-13 GTACATG 10005 0.0 23.056799 1 TACATGG 9905 0.0 22.71399 2 ACATGGG 10055 0.0 21.574348 3 GTACTTT 9890 0.0 20.847546 14-15 AGTACTT 9100 0.0 20.601347 12-13 TACATCG 120 0.0048440434 19.735268 5 CATGGGA 6410 0.0 19.137978 4 GTATCAA 22870 0.0 19.05269 1 GTATAGG 580 0.0 18.822603 1 ACTTTTT 11635 0.0 18.39451 16-17 CCGACGC 285 0.0 18.358809 94-95 GGTATCA 18345 0.0 18.085865 1 ATGGGAT 2630 0.0 17.46909 5 AGAGTAC 16625 0.0 17.298582 10-11 CAACGCA 25335 0.0 17.01276 5 CATGGGG 3625 0.0 16.985943 4 TCAACGC 25535 0.0 16.916607 4 ATCAACG 25560 0.0 16.88153 3 AACGCAG 25560 0.0 16.872448 6 >>END_MODULE