FastQCFastQC Report
Fri 27 May 2016
SRR938979_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938979_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2045521
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108070.528325057528131No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94480.4618872160197817No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT81230.39711154273165616No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61280.2995813780450066No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT42070.20566887360237315No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT32040.15663491110577696No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA25960.12691143234413138No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC24970.12207158958524503No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT23670.1157162405079195No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC22280.10892090572524066No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC21440.10481437247527646No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA20860.10197890904077739No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20820.10178335983839813No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCCG550.002591911234.513284
GTACATG95200.023.5650391
TACATGG94600.022.8249132
GAGTACT92550.022.69148412-13
ACATGGG92550.021.7409423
GTACTTT103000.020.25130514-15
AGTACTT97650.019.99970812-13
GTATAGG6800.018.9120711
GGTATCA186750.018.5420631
CATGGGA61050.018.500364
GTATCAA230450.018.3949031
AGTACAT75250.017.5948932
ACTTTTT121200.017.38563216-17
AGAGTAC175150.017.36875310-11
CATGGGG34250.017.3196184
GTGTACG1952.2292329E-417.0980911
CGCCGTA1154.112764E-516.59661590-91
CAACGCA257400.016.2979375
TCAACGC258150.016.2873524
ATCAACG259100.016.2459493
Produced by FastQC (version 0.11.5)