##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938979_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2045521 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.167087015972946 33.0 31.0 34.0 30.0 34.0 2 32.394853438317185 34.0 31.0 34.0 31.0 34.0 3 32.478295749591425 34.0 31.0 34.0 30.0 34.0 4 36.03765299891813 37.0 35.0 37.0 35.0 37.0 5 35.96656695286922 37.0 35.0 37.0 35.0 37.0 6 36.01531345803832 37.0 35.0 37.0 35.0 37.0 7 35.94925938183964 37.0 35.0 37.0 35.0 37.0 8 35.9277475029589 37.0 35.0 37.0 35.0 37.0 9 37.594286736728684 39.0 37.0 39.0 35.0 39.0 10-11 37.50951273538624 39.0 37.0 39.0 34.5 39.0 12-13 37.426411657470155 39.0 37.0 39.0 34.0 39.0 14-15 38.70810175011647 40.0 38.0 41.0 34.0 41.0 16-17 38.79686153307641 40.0 38.0 41.0 34.5 41.0 18-19 38.861890931454624 40.0 38.0 41.0 35.0 41.0 20-21 38.846954638940396 40.0 38.0 41.0 34.5 41.0 22-23 38.770500033976674 40.0 38.0 41.0 34.0 41.0 24-25 38.69378803737532 40.0 38.0 41.0 34.0 41.0 26-27 38.526827395074406 40.0 38.0 41.0 34.0 41.0 28-29 38.366133615836745 40.0 38.0 41.0 34.0 41.0 30-31 38.15319544507243 40.0 37.5 41.0 33.5 41.0 32-33 38.02182011331098 40.0 37.0 41.0 33.0 41.0 34-35 37.90968120102409 40.0 37.0 41.0 33.0 41.0 36-37 37.75494605041943 40.0 37.0 41.0 33.0 41.0 38-39 37.602155392195925 40.0 37.0 41.0 33.0 41.0 40-41 37.43089755617273 40.0 37.0 41.0 32.0 41.0 42-43 37.23459524492782 40.0 36.5 41.0 31.5 41.0 44-45 36.946453250785495 40.0 36.0 41.0 31.0 41.0 46-47 36.58060440347471 40.0 36.0 41.0 30.0 41.0 48-49 36.20814061552045 39.0 35.5 41.0 29.5 41.0 50-51 35.941069781243996 39.0 35.0 41.0 29.0 41.0 52-53 35.692721560912844 39.0 35.0 40.0 28.0 41.0 54-55 35.41074474424853 39.0 35.0 40.0 27.5 41.0 56-57 35.30057085700905 38.5 35.0 40.0 27.0 41.0 58-59 35.04644757985862 38.0 34.5 40.0 26.5 41.0 60-61 34.69675182997388 38.0 34.0 40.0 26.0 41.0 62-63 34.304042588660785 37.5 34.0 40.0 26.0 41.0 64-65 33.914590463749825 37.0 33.5 40.0 25.0 41.0 66-67 33.47547813002164 36.5 33.0 39.0 23.0 40.5 68-69 32.99781595984593 36.0 32.5 39.0 22.0 40.0 70-71 32.47558739313847 35.0 32.0 38.5 21.5 40.0 72-73 31.970469626075705 35.0 31.0 37.5 19.5 40.0 74-75 31.444210301434207 35.0 31.0 37.0 18.0 39.0 76-77 29.519718448258416 33.0 28.5 35.0 13.0 37.5 78-79 30.477043990259695 34.0 30.0 36.0 13.0 38.0 80-81 30.449894183437863 34.0 30.0 36.0 11.5 37.5 82-83 30.029604193748195 34.0 30.0 35.0 7.0 37.0 84-85 29.57119017599917 34.0 29.5 35.0 3.5 36.5 86-87 28.88431260299943 34.0 29.0 35.0 2.0 36.0 88-89 28.189097300883247 33.0 27.5 35.0 2.0 35.5 90-91 27.61028535028484 33.0 26.5 35.0 2.0 35.0 92-93 27.217522088504595 33.0 26.0 35.0 2.0 35.0 94-95 26.677503677547186 32.5 25.5 34.0 2.0 35.0 96-97 25.843861539431764 32.0 24.5 34.0 2.0 35.0 98-99 24.674927316805842 32.0 11.0 34.0 2.0 35.0 100-101 19.213697879415562 24.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1846920900470721 1101 2 0.14468203176831906 1101 3 0.263537817386748 1101 4 0.16813398004199342 1101 5 0.05789019196576817 1101 6 0.1430159579221808 1101 7 0.21678221850900314 1101 8 0.1782223081005796 1101 9 0.2533435524704899 1101 10-11 0.2578760964597251 1101 12-13 0.2834706195764696 1101 14-15 0.26714995841977895 1101 16-17 0.2892272348710918 1101 18-19 0.29129942879622206 1101 20-21 0.27441935818514906 1101 22-23 0.3685754867807489 1101 24-25 0.3397169185884863 1101 26-27 0.29619582621842966 1101 28-29 0.27882391604099865 1101 30-31 0.25421416880472947 1101 32-33 0.3442259736181299 1101 34-35 0.40649348186613565 1101 36-37 0.4485809648831349 1101 38-39 0.4587806781080843 1101 40-41 0.49789199708737897 1101 42-43 0.3513289065344125 1101 44-45 0.2971031464078848 1101 46-47 0.24538969781070818 1101 48-49 0.18319980884937337 1101 50-51 0.03127184094221036 1101 52-53 0.04937277311675814 1101 54-55 0.1811213113832153 1101 56-57 0.07809116147928563 1101 58-59 0.20554401114739562 1101 60-61 0.1879435835745582 1101 62-63 0.17591873135738467 1101 64-65 0.08300457369605141 1101 66-67 0.05511534626983661 1101 68-69 0.16167362160494747 1101 70-71 0.23545986393764196 1101 72-73 0.2763447027105954 1101 74-75 0.36994916710079195 1101 76-77 0.07594276366522834 1101 78-79 0.332057429265177 1101 80-81 0.33690417689953733 1101 82-83 0.16357240535768724 1101 84-85 0.3265950041922068 1101 86-87 0.5002495099870146 1101 88-89 0.652959754956747 1101 90-91 0.6122491130029815 1101 92-93 0.5642266408603049 1101 94-95 0.4040256555051478 1101 96-97 0.2628506727445483 1101 98-99 0.37951000659372625 1101 100-101 0.9768340015632653 1102 1 0.12561412530931904 1102 2 0.1525870741378199 1102 3 0.1816136211585544 1102 4 0.1004745871242676 1102 5 0.0327581233004679 1102 6 0.041634396546243124 1102 7 0.056135385555485584 1102 8 0.09322142359605579 1102 9 0.1483528657828046 1102 10-11 0.2824046208633746 1102 12-13 0.21994671887684092 1102 14-15 0.2921771005683311 1102 16-17 0.1976879541987273 1102 18-19 0.25844962460124776 1102 20-21 0.21251011181768575 1102 22-23 0.28655937145523325 1102 24-25 0.3022850341082446 1102 26-27 0.2342007308827121 1102 28-29 0.2310233839508271 1102 30-31 0.09943975214412859 1102 32-33 0.3261907529949539 1102 34-35 0.3183976986980852 1102 36-37 0.29851176541176017 1102 38-39 0.35716411602106746 1102 40-41 0.2368287532797808 1102 42-43 0.38433306543998214 1102 44-45 0.26681060087324227 1102 46-47 0.22525334536860697 1102 48-49 0.2617169283476741 1102 50-51 0.1571984027864204 1102 52-53 0.27898581106026654 1102 54-55 0.3463207411379514 1102 56-57 0.2896169491218856 1102 58-59 0.13797046719792405 1102 60-61 0.2523398485374244 1102 62-63 0.2436448956948567 1102 64-65 0.36250800245181125 1102 66-67 0.19696298949887137 1102 68-69 0.24033295555570078 1102 70-71 0.33926167248923633 1102 72-73 0.5189703630539242 1102 74-75 0.44013720235610165 1102 76-77 0.17276305662700153 1102 78-79 0.3421364450024207 1102 80-81 0.2946508756164832 1102 82-83 0.26473197344199306 1102 84-85 0.6117271769168582 1102 86-87 0.5711616249240379 1102 88-89 0.3987635614479714 1102 90-91 0.39159211691908524 1102 92-93 0.5539108900894014 1102 94-95 0.6693449269509095 1102 96-97 0.69061526967063 1102 98-99 0.8740711709485396 1102 100-101 1.0566137079077649 1103 1 0.017213304550203645 1103 2 0.01349179945363943 1103 3 0.022818202427295375 1103 4 0.017039300666624513 1103 5 0.07875225523451945 1103 6 0.04073796750182623 1103 7 0.09675035980028923 1103 8 0.1576680500415577 1103 9 0.13525735889637502 1103 10-11 0.10315688031380432 1103 12-13 0.10519198688082554 1103 14-15 0.11374746455994256 1103 16-17 0.08631867852389519 1103 18-19 0.21610742732617894 1103 20-21 0.30250861242124216 1103 22-23 0.1696849187786924 1103 24-25 0.04975035400185135 1103 26-27 0.07459129156946886 1103 28-29 0.03066828369838248 1103 30-31 0.19561582446054615 1103 32-33 0.1562669830105321 1103 34-35 0.07869904821323104 1103 36-37 0.03372243644709272 1103 38-39 0.10754348665962254 1103 40-41 0.05780315093955579 1103 42-43 0.1359072858559287 1103 44-45 0.10171552922017923 1103 46-47 -0.07104970890778617 1103 48-49 -0.037901571520770005 1103 50-51 0.05233968735494443 1103 52-53 0.105098558672168 1103 54-55 0.16506558878585764 1103 56-57 0.13953742860203278 1103 58-59 0.09957308279876287 1103 60-61 -0.01274410891238631 1103 62-63 0.1135018054329393 1103 64-65 -0.043667994019649825 1103 66-67 -0.19924493769930507 1103 68-69 -0.12128631676444712 1103 70-71 0.145236563141232 1103 72-73 0.016860998127079085 1103 74-75 0.06429685482283887 1103 76-77 -0.005651702101438616 1103 78-79 -0.047103810233362964 1103 80-81 0.11472373928284085 1103 82-83 0.18959310424361675 1103 84-85 0.11365538908204087 1103 86-87 0.4588986914628208 1103 88-89 0.30665744618580604 1103 90-91 0.403389945769689 1103 92-93 0.4693045283311257 1103 94-95 0.4067222841975777 1103 96-97 0.2608128399482794 1103 98-99 0.6611748154153894 1103 100-101 0.9819266996237452 1104 1 0.09468445054056218 1104 2 0.05810475873007448 1104 3 0.06586159563581617 1104 4 -0.011645842539024898 1104 5 -0.057786759529065534 1104 6 0.0651551337676608 1104 7 0.038313654508179695 1104 8 0.0975610917087053 1104 9 0.05543301451240268 1104 10-11 0.12407013992509519 1104 12-13 0.11262958972537973 1104 14-15 0.1594990496605746 1104 16-17 0.13816312002764164 1104 18-19 0.13840268755885177 1104 20-21 0.19276671628416153 1104 22-23 0.18146601379267935 1104 24-25 0.20290760979278843 1104 26-27 0.14793096338747347 1104 28-29 0.16223307432701262 1104 30-31 -0.05769519472175233 1104 32-33 0.16373493578546316 1104 34-35 0.2866182224649876 1104 36-37 0.18675455271466035 1104 38-39 0.2182026411191913 1104 40-41 0.2325177752908445 1104 42-43 0.06282465349571709 1104 44-45 0.2913085237082882 1104 46-47 0.2090270721009162 1104 48-49 0.21542541373866442 1104 50-51 0.3161322204090382 1104 52-53 0.07903429736933276 1104 54-55 0.27819948519908877 1104 56-57 0.2028534245975706 1104 58-59 0.2929083243899626 1104 60-61 0.2200149730525638 1104 62-63 0.03305967455138159 1104 64-65 0.0012319231293602684 1104 66-67 -0.07311149006228135 1104 68-69 0.12059730635559163 1104 70-71 0.07644923704202 1104 72-73 0.02693904491488297 1104 74-75 0.028557294623535512 1104 76-77 0.05426423866009955 1104 78-79 0.153395597151885 1104 80-81 0.07931227477643787 1104 82-83 -0.0669993033832732 1104 84-85 0.091493467169105 1104 86-87 0.13705824867027872 1104 88-89 0.0010943470983200143 1104 90-91 0.2838081994548318 1104 92-93 0.42724772361782826 1104 94-95 0.4552833235172784 1104 96-97 0.34884101504004406 1104 98-99 0.4379838726401104 1104 100-101 0.44076298835946304 1105 1 -0.054184568551534085 1105 2 -0.04434388877390916 1105 3 0.01018356436178891 1105 4 0.030799517067769955 1105 5 -0.015683285503456545 1105 6 0.06690022699905285 1105 7 0.08019617069689389 1105 8 0.06871648107374995 1105 9 0.03381620499867921 1105 10-11 0.08608183146461101 1105 12-13 0.16127542189773436 1105 14-15 0.11332972852775214 1105 16-17 0.07361236381127867 1105 18-19 0.12890428692579547 1105 20-21 0.07868114179378694 1105 22-23 0.023651824565831703 1105 24-25 -0.010809330122278027 1105 26-27 -0.0639677415153912 1105 28-29 -0.011238332112810667 1105 30-31 -0.01534554635957619 1105 32-33 0.010622160911687217 1105 34-35 -0.040046066513319545 1105 36-37 0.07328922052558795 1105 38-39 0.14214786698101278 1105 40-41 0.20733777685119748 1105 42-43 0.106298456199589 1105 44-45 0.11666322651581851 1105 46-47 0.11053769368067634 1105 48-49 0.1744674096594352 1105 50-51 0.10074026405448677 1105 52-53 0.050694991951552026 1105 54-55 0.15174166501920894 1105 56-57 0.13244951478626632 1105 58-59 0.1489523432034261 1105 60-61 0.04649522337856382 1105 62-63 0.04352981598668748 1105 64-65 0.12375137187716945 1105 66-67 0.1927581866092467 1105 68-69 0.1769355641044399 1105 70-71 0.20923375160349877 1105 72-73 0.17056930137086823 1105 74-75 0.19431050171647968 1105 76-77 -0.019862518135816742 1105 78-79 0.03766161589847172 1105 80-81 0.16502176398210366 1105 82-83 0.25752896695989236 1105 84-85 0.10506509874629444 1105 86-87 0.2520499453366867 1105 88-89 0.5118312818864972 1105 90-91 0.3761620273473447 1105 92-93 0.5593109905616416 1105 94-95 0.5074706110578227 1105 96-97 0.48854697114940393 1105 98-99 0.5090103931532433 1105 100-101 0.7601543372889417 1106 1 -0.013191217707721137 1106 2 0.0012204596124831824 1106 3 -0.06395354917814444 1106 4 -0.019424896578570383 1106 5 0.05894145396685957 1106 6 0.023587943952946944 1106 7 0.03570882809171394 1106 8 0.042190951940149546 1106 9 -0.037283884386191346 1106 10-11 0.09763380868864857 1106 12-13 0.07743732745000642 1106 14-15 0.009058918265246518 1106 16-17 -0.10957852284330727 1106 18-19 -0.09884477363443978 1106 20-21 -0.14315337303030873 1106 22-23 -0.15376247881096816 1106 24-25 0.03935746701846199 1106 26-27 -0.04525585628747564 1106 28-29 -0.05475438729568083 1106 30-31 -0.07767259773182644 1106 32-33 -0.0468467354256461 1106 34-35 0.08897882963160697 1106 36-37 0.15882660544454552 1106 38-39 0.12620685993963576 1106 40-41 0.18227248254979145 1106 42-43 0.10208544744980941 1106 44-45 0.33960381966981856 1106 46-47 0.34338013262435396 1106 48-49 0.24979055103818837 1106 50-51 0.2910149414945522 1106 52-53 0.4154382628458535 1106 54-55 0.47987466822934977 1106 56-57 0.4067330218236691 1106 58-59 0.30740394454583964 1106 60-61 0.3028393820956552 1106 62-63 0.384737272936853 1106 64-65 0.31538241586182636 1106 66-67 0.32015396284365494 1106 68-69 0.3773315850768455 1106 70-71 0.29146254833299423 1106 72-73 0.43897314973056467 1106 74-75 0.5349794557760355 1106 76-77 0.404879885867949 1106 78-79 0.553314011820067 1106 80-81 0.4984061945222322 1106 82-83 0.5222260653609538 1106 84-85 0.6602787581925718 1106 86-87 0.7535019869960635 1106 88-89 0.8309975181913494 1106 90-91 0.8660910321834336 1106 92-93 0.9224736398425719 1106 94-95 0.9736260677902031 1106 96-97 1.1451763844283676 1106 98-99 1.2933049001858912 1106 100-101 1.3389369798144735 1107 1 0.007824402310760092 1107 2 0.0333496423170061 1107 3 0.008171504084572234 1107 4 -0.021585768700482788 1107 5 -0.0039561186703238604 1107 6 0.007236164092681463 1107 7 -0.016943073121517216 1107 8 0.04230329704812874 1107 9 -0.016150352610239338 1107 10-11 0.06099741110032397 1107 12-13 0.00935593898592657 1107 14-15 0.032226807401876556 1107 16-17 0.05742222713620038 1107 18-19 0.03810872055144898 1107 20-21 0.08868745723040661 1107 22-23 0.08787540153035422 1107 24-25 0.03634995811435715 1107 26-27 0.04979746715410016 1107 28-29 0.10930033995368404 1107 30-31 0.09757033591269959 1107 32-33 0.0906307829012789 1107 34-35 0.11236887868897583 1107 36-37 0.09774603780823554 1107 38-39 0.20789592309277083 1107 40-41 0.13778837432197832 1107 42-43 0.18949829143079455 1107 44-45 0.2458988905535051 1107 46-47 0.2637196071528862 1107 48-49 0.17814068721867926 1107 50-51 0.13249700625593874 1107 52-53 0.023213910677341687 1107 54-55 0.16775592046233356 1107 56-57 0.1616916413755618 1107 58-59 0.045146688418221004 1107 60-61 0.1108787879013633 1107 62-63 0.28966699651728334 1107 64-65 0.3023493712183054 1107 66-67 0.24033149847780777 1107 68-69 0.11734046135249088 1107 70-71 0.10760067006449248 1107 72-73 0.03662635151552962 1107 74-75 0.03492480139918186 1107 76-77 -0.037172305013474016 1107 78-79 0.23217982216735678 1107 80-81 0.19444410644164378 1107 82-83 0.12109583308997429 1107 84-85 0.2953643884435664 1107 86-87 0.15996633463029042 1107 88-89 0.24781962932798862 1107 90-91 0.2896376559093028 1107 92-93 0.5710826859516338 1107 94-95 0.6377498720991959 1107 96-97 0.7689660177076263 1107 98-99 0.7893520729858245 1107 100-101 0.7874039751496191 1108 1 0.13667510932452842 1108 2 0.14417977793578984 1108 3 0.18966443919550358 1108 4 0.140086847263575 1108 5 0.107330645861893 1108 6 0.1316895871562167 1108 7 0.15498059951859489 1108 8 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#Quality Count 3 7.0 4 15.0 5 58.0 6 138.0 7 288.0 8 747.0 9 1258.0 10 2100.0 11 3402.0 12 5204.0 13 6991.0 14 8729.0 15 11297.0 16 15186.0 17 21902.0 18 21107.0 19 13488.0 20 12020.0 21 12632.0 22 13691.0 23 15285.0 24 17427.0 25 20518.0 26 24427.0 27 29563.0 28 35908.0 29 43179.0 30 52671.0 31 63447.0 32 78681.0 33 100149.0 34 133870.0 35 191656.0 36 295556.0 37 454118.0 38 320320.0 39 18486.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.15551452580703 20.82745616540682 15.10046889738931 21.916560411396844 2 15.217247830748255 22.349073903421182 39.46798884000702 22.965689425823545 3 18.844049999975557 26.416155101805362 31.424952371547395 23.31484252667169 4 12.31299996431227 18.200937560650807 38.60522575910978 30.88083671592714 5 12.43644039831417 38.810063548602045 34.95578876970708 13.797707283376702 6 29.21387754024525 38.16465340614934 18.004361724959068 14.617107328646345 7 25.95069911284216 34.642763383998506 22.121307969949953 17.285229533209385 8 25.11653510279288 35.09057105744698 20.826967799401714 18.965926040358422 9 25.62926511143127 17.151816089886147 21.930500835728402 35.288417962954185 10-11 23.9196762096307 27.41942028461209 28.189835254685725 20.471068251071486 12-13 25.334988005500797 25.369991312726686 28.593179928243217 20.701840753529297 14-15 22.148173515479463 26.594346958116116 27.75934300676045 23.49813651964397 16-17 20.40939745129419 29.73754925213991 28.588448764452366 21.26460453211353 18-19 20.24945343757455 28.436719021936806 31.107932879396454 20.205894661092184 20-21 21.4678558665494 27.230373093211952 30.77641344185662 20.525357598382026 22-23 20.98790479295984 27.295442090303645 30.739870184662 20.976782932074517 24-25 21.06514802970494 27.474843175458936 30.92179317859995 20.538215616236176 26-27 20.862000649712385 27.347953735980646 31.368470763309386 20.42157485099758 28-29 20.339805848974418 27.908953269118236 31.255166776581618 20.496074105325736 30-31 20.740608382900984 27.532594385489073 30.936690456856713 20.790106774753227 32-33 20.573878244222378 27.658381409919524 30.954656539825304 20.81308380603279 34-35 21.2250453853612 27.544134910766978 30.805500319518806 20.425319384353013 36-37 21.116412575350267 27.188785508298768 31.015864051060316 20.67893786529065 38-39 20.752615516400084 27.73420349348309 30.991852516068597 20.521328474048232 40-41 20.803250432252636 27.65888844154133 30.916434253720382 20.621426872485657 42-43 20.59691254827581 27.852040313707505 30.30162434545471 21.249422792561976 44-45 20.827414794428396 27.769087883794484 30.147332202547126 21.25616511922999 46-47 21.231670421660052 28.0235844709737 29.750425140864195 20.994319966502054 48-49 21.11645222756334 28.082490304712525 29.66138299471633 21.139674473007805 50-51 20.77550849800502 28.346091768334492 29.772900101417278 21.105499632243212 52-53 21.030118977023456 28.706574021972887 29.09165928875822 21.171647712245438 54-55 20.659724344066866 28.23473824028206 29.341008965442057 21.764528450209017 56-57 21.068666613542465 28.484039029665304 29.016323958541612 21.43097039825062 58-59 21.24792777131102 28.13146693013894 29.130463265025767 21.490142033524272 60-61 21.04330553720895 28.36206892337236 29.449143945530874 21.14548159388782 62-63 20.80744407884364 28.251962914536676 29.58609214460192 21.35450086201776 64-65 20.649717306973095 28.364535897003428 29.386630685331983 21.59911611069149 66-67 20.764169099081943 28.34237814558901 28.862211425429983 22.031241329899068 68-69 20.646983736184655 28.281399645361123 29.124608095681122 21.947008522773107 70-71 20.990853423787943 28.27562875836676 28.844579579906227 21.888938237939065 72-73 20.918582600716395 27.86507691683439 29.103661121054248 22.112679361394967 74-75 20.597050824704315 28.77266965237707 28.52373551774829 22.10654400517032 76-77 20.778722245505662 28.112761666533327 28.847719197291323 22.260796890669685 78-79 20.628984134107927 28.163901758873443 28.910091974556295 22.29702213246233 80-81 20.750134012738606 28.386134637520293 28.591999714497256 22.271731635243846 82-83 20.709222735919113 28.109488976158154 28.787433617156704 22.393854670766032 84-85 20.605631286150675 28.062738127276777 28.623696143545025 22.707934443027526 86-87 20.495542112492117 28.147152124035014 28.648380055400036 22.708925708072837 88-89 20.62706367852154 27.80735154403046 28.532317972814063 23.033266804633932 90-91 20.524558900678365 27.717938865177434 28.63417109446811 23.123331139676097 92-93 20.695583179053163 27.990032857154727 28.46822398792288 22.846159975869227 94-95 20.55686588350094 27.659476172469194 28.5459091572409 23.237748786788963 96-97 20.273739617862542 27.529985644240675 28.767592404964898 23.42868233293189 98-99 20.330028388855457 27.703895486773295 28.173873551041517 23.792202573329728 100-101 20.472854949203402 26.949671744615404 28.98251341596305 23.594959890218142 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1887.0 1 1315.0 2 784.5 3 859.5 4 804.5 5 814.5 6 1356.0 7 2468.0 8 3685.0 9 4267.0 10 3730.0 11 3087.5 12 3207.0 13 3467.5 14 3721.0 15 3917.0 16 4041.5 17 4275.0 18 4513.5 19 4851.0 20 5439.0 21 6367.5 22 7494.0 23 8956.5 24 10703.5 25 12949.5 26 16135.5 27 19373.5 28 21585.5 29 24468.0 30 28773.0 31 33145.0 32 37846.0 33 43691.0 34 50325.0 35 55122.5 36 60202.5 37 65754.0 38 68163.5 39 68562.5 40 68290.0 41 67932.5 42 67652.0 43 68553.5 44 70247.5 45 70766.5 46 70706.5 47 76411.5 48 93051.5 49 91993.0 50 74762.0 51 66489.5 52 60648.0 53 56444.5 54 52357.0 55 47399.5 56 42394.0 57 37341.5 58 32857.5 59 28132.5 60 23417.0 61 19304.0 62 15619.5 63 11776.0 64 8358.0 65 6029.0 66 4344.5 67 3431.5 68 2583.0 69 1643.0 70 1001.0 71 676.0 72 509.0 73 393.0 74 301.0 75 234.0 76 161.0 77 75.0 78 32.0 79 13.5 80 8.5 81 4.5 82 3.0 83 3.0 84 1.5 85 0.0 86 0.5 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.389876222243624 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0010021896621936416 16-17 0.0025176959806328066 18-19 4.399857053533061E-4 20-21 0.0 22-23 0.0 24-25 1.2221825148702947E-4 26-27 2.4443650297405893E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.012612923553461441 36-37 0.023881446340565556 38-39 0.011097417235022276 40-41 0.006428680028217749 42-43 0.005499821316916326 44-45 0.0020288229746846893 46-47 0.0010999642633832652 48-49 0.0033487800907446076 50-51 5.622039568403355E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 8.799714107066122E-4 60-61 0.0015643936190339772 62-63 0.0014421753675469477 64-65 7.821968095169886E-4 66-67 0.0028110197842016777 68-69 0.002933238035688707 70-71 7.333095089221767E-5 72-73 0.0 74-75 0.0 76-77 0.09119925925962138 78-79 0.0031287872380679543 80-81 3.1776745386627664E-4 82-83 0.0 84-85 1.2221825148702947E-4 86-87 0.08995263309445369 88-89 0.4305015690379126 90-91 0.38268978905618667 92-93 0.0 94-95 9.533023615988298E-4 96-97 2.4443650297405893E-5 98-99 0.0 100-101 4.888730059481179E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2045521.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.515775026375735 #Duplication Level Percentage of deduplicated Percentage of total 1 69.5578262804614 31.659783723052065 2 15.104973800478838 13.750291785637888 3 6.122601851071008 8.360249052882589 4 2.9869218711527217 5.438082556349941 5 1.6523281039669158 3.760349712495805 6 1.044274800154093 2.8518586121716303 7 0.6867397759282077 2.1880245199968384 8 0.49824156247442203 1.8142280693100576 9 0.33770386901640886 1.383376799491877 >10 1.8077063661320256 14.746506811406332 >50 0.12968403826476047 4.053119251099476 >100 0.0653968037982227 5.274622580438472 >500 0.002908147725471727 0.9568634267665993 >1k 0.0022618926753668984 2.070997981367364 >5k 3.23127525052414E-4 1.1618355544083223 >10k+ 1.0770917501747135E-4 0.5298095631246356 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10807 0.528325057528131 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9448 0.4618872160197817 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8123 0.39711154273165616 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6128 0.2995813780450066 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4207 0.20566887360237315 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3204 0.15663491110577696 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2596 0.12691143234413138 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2497 0.12207158958524503 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2367 0.1157162405079195 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2228 0.10892090572524066 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2144 0.10481437247527646 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2086 0.10197890904077739 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2082 0.10178335983839813 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.4443650297405893E-5 0.0 16-17 0.0 0.0 0.0 1.2221825148702947E-4 0.0 18-19 0.0 0.0 0.0 1.4666190178443537E-4 0.0 20-21 0.0 0.0 0.0 2.4443650297405894E-4 0.0 22-23 0.0 0.0 0.0 4.888730059481179E-4 0.0 24-25 0.0 0.0 0.0 7.821968095169886E-4 0.0 26-27 0.0 0.0 0.0 0.0021999285267665304 0.0 28-29 0.0 0.0 0.0 0.007846411745467291 0.0 30-31 0.0 2.4443650297405893E-5 0.0 0.020166011495359862 0.0 32-33 0.0 4.8887300594811786E-5 0.0 0.04641849191477379 0.0 34-35 0.0 4.8887300594811786E-5 0.0 0.08997707674475108 0.0 36-37 0.0 4.8887300594811786E-5 0.0 0.16069255705514635 0.0 38-39 0.0 4.8887300594811786E-5 0.0 0.26308700815097963 0.0 40-41 0.0 4.8887300594811786E-5 0.0 0.37550335586874933 0.0 42-43 0.0 4.8887300594811786E-5 0.0 0.48985075196001404 0.0 44-45 0.0 4.8887300594811786E-5 0.0 0.6344104998188725 0.0 46-47 0.0 4.8887300594811786E-5 0.0 0.809744803402165 0.0 48-49 0.0 4.8887300594811786E-5 0.0 0.9939765956937132 0.0 50-51 0.0 4.8887300594811786E-5 0.0 1.188425833809577 0.0 52-53 0.0 4.8887300594811786E-5 0.0 1.383241726679902 0.0 54-55 0.0 4.8887300594811786E-5 0.0 1.601547967486034 0.0 56-57 0.0 4.8887300594811786E-5 0.0 1.8669815660655646 0.0 58-59 0.0 4.8887300594811786E-5 0.0 2.1514567682267742 0.0 60-61 0.0 4.8887300594811786E-5 0.0 2.469248665743349 0.0 62-63 0.0 4.8887300594811786E-5 0.0 2.787847203719737 0.0 64-65 0.0 4.8887300594811786E-5 0.0 3.1238496207078783 0.0 66-67 0.0 4.8887300594811786E-5 0.0 3.5094237604991587 0.0 68-69 0.0 4.8887300594811786E-5 0.0 3.9337899733124226 0.0 70-71 0.0 4.8887300594811786E-5 0.0 4.3983415472146214 0.0 72-73 0.0 4.8887300594811786E-5 0.0 4.862208698908493 0.0 74-75 0.0 4.8887300594811786E-5 0.0 5.3614946998833055 0.0 76-77 0.0 4.8887300594811786E-5 0.0 5.892264122441178 0.0 78-79 0.0 4.8887300594811786E-5 0.0 6.506484167114393 0.0 80-81 0.0 4.8887300594811786E-5 0.0 7.161598438735169 0.0 82-83 0.0 4.8887300594811786E-5 0.0 7.812043973149139 0.0 84-85 0.0 4.8887300594811786E-5 0.0 8.488644213381335 0.0 86-87 0.0 4.8887300594811786E-5 0.0 9.20838749638845 0.0 88-89 0.0 4.8887300594811786E-5 0.0 9.981711260847481 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATACCCG 55 0.0025919112 34.51328 4 GTACATG 9520 0.0 23.565039 1 TACATGG 9460 0.0 22.824913 2 GAGTACT 9255 0.0 22.691484 12-13 ACATGGG 9255 0.0 21.740942 3 GTACTTT 10300 0.0 20.251305 14-15 AGTACTT 9765 0.0 19.999708 12-13 GTATAGG 680 0.0 18.912071 1 GGTATCA 18675 0.0 18.542063 1 CATGGGA 6105 0.0 18.50036 4 GTATCAA 23045 0.0 18.394903 1 AGTACAT 7525 0.0 17.594893 2 ACTTTTT 12120 0.0 17.385632 16-17 AGAGTAC 17515 0.0 17.368753 10-11 CATGGGG 3425 0.0 17.319618 4 GTGTACG 195 2.2292329E-4 17.098091 1 CGCCGTA 115 4.112764E-5 16.596615 90-91 CAACGCA 25740 0.0 16.297937 5 TCAACGC 25815 0.0 16.287352 4 ATCAACG 25910 0.0 16.245949 3 >>END_MODULE