FastQCFastQC Report
Fri 27 May 2016
SRR938978_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938978_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2059780
Sequences flagged as poor quality0
Sequence length101
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113000.5486022779131752No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92640.4497567701405004No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT76700.3723698647428366No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57980.28148637233102564No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT44740.21720766295429608No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT34100.16555166085698472No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC27000.13108196020934273No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA25670.12462495994717883No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT22470.10908932021866412No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG909.142867E-426.292672
GTACATG97500.024.8487821
TACATGG98200.024.0489582
ACATGGG98350.023.0498623
GAGTACT88850.022.71820612-13
ATCACGC1050.002243936522.5365733
CTACACG3950.021.5666454
TAGGACG2457.850758E-921.248774
AGTACTT93500.020.90503512-13
GTACTTT97500.020.63013514-15
CATGGGG33700.019.9418584
CATGGGA64150.019.4028844
CCCTATA4700.019.1321132
GTAGGAC5950.019.0898043
GTATCAA229350.018.7291581
GGTATCA185150.017.7203041
AGAGTAC171250.017.3278310-11
ACTTTTT119350.017.17176216-17
AGTACAT74400.016.7933832
TACTTTT116550.016.66934814-15
Produced by FastQC (version 0.11.5)