##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938978_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2059780 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.787519055433105 33.0 31.0 34.0 30.0 34.0 2 31.847332239365368 33.0 31.0 34.0 30.0 34.0 3 31.88626261057006 34.0 31.0 34.0 30.0 34.0 4 35.435325617298936 37.0 35.0 37.0 33.0 37.0 5 35.48520812902349 37.0 35.0 37.0 33.0 37.0 6 35.568012117798986 37.0 35.0 37.0 33.0 37.0 7 35.48807785297459 37.0 35.0 37.0 33.0 37.0 8 35.47640961656099 37.0 35.0 37.0 33.0 37.0 9 37.07404577187855 39.0 37.0 39.0 33.0 39.0 10-11 36.96250182058278 39.0 37.0 39.0 33.0 39.0 12-13 36.846085747021526 39.0 37.0 39.0 33.0 39.0 14-15 38.03083533192866 40.0 38.0 41.0 33.0 41.0 16-17 37.96978585091612 40.0 38.0 41.0 33.0 41.0 18-19 37.95067264465137 40.0 38.0 41.0 33.0 41.0 20-21 37.843404635446504 40.0 38.0 41.0 32.0 41.0 22-23 37.91234986260669 40.0 38.0 41.0 33.0 41.0 24-25 37.840398489159035 40.0 38.0 41.0 32.0 41.0 26-27 37.755691384516794 40.0 37.5 41.0 32.0 41.0 28-29 37.62830909126217 40.0 37.0 41.0 32.0 41.0 30-31 37.42673368029595 40.0 37.0 41.0 32.0 41.0 32-33 37.21719067084834 40.0 36.5 41.0 31.0 41.0 34-35 36.889312693588636 40.0 36.0 41.0 30.5 41.0 36-37 36.61118104846149 39.5 35.5 41.0 30.0 41.0 38-39 36.39804299488295 39.0 35.0 41.0 30.0 41.0 40-41 36.24880618318461 39.0 35.0 41.0 30.0 41.0 42-43 36.02727742768646 39.0 35.0 41.0 29.0 41.0 44-45 35.896491858353805 39.0 35.0 41.0 28.0 41.0 46-47 35.6169486547107 39.0 35.0 41.0 26.5 41.0 48-49 35.39889915427861 39.0 35.0 41.0 25.5 41.0 50-51 34.22691792327336 38.0 34.0 40.0 23.5 40.5 52-53 34.38079892027304 38.0 34.0 39.5 24.0 40.5 54-55 34.893196846265134 39.0 35.0 40.5 24.0 41.0 56-57 34.80896090844654 39.0 35.0 41.0 23.5 41.0 58-59 34.62491746691394 38.5 34.0 40.5 23.0 41.0 60-61 34.369096699647535 38.0 34.0 40.0 23.0 41.0 62-63 34.035258377108235 38.0 34.0 40.0 22.0 41.0 64-65 33.644506209401 37.0 33.5 40.0 20.5 41.0 66-67 33.261834759051936 37.0 33.0 40.0 19.5 41.0 68-69 32.872029294390664 36.0 33.0 39.0 18.5 41.0 70-71 32.44319417607706 36.0 32.0 39.0 15.5 40.5 72-73 32.00478594801386 35.0 32.0 38.5 12.5 40.0 74-75 31.530362223150043 35.0 31.0 37.5 8.0 39.5 76-77 31.109309974851683 35.0 31.0 37.0 7.0 39.0 78-79 30.65846449620833 35.0 31.0 36.5 2.0 39.0 80-81 30.195412616881413 34.5 30.0 36.0 2.0 38.5 82-83 29.79011520647836 34.0 30.0 35.5 2.0 37.0 84-85 29.40552024973541 34.0 29.5 35.0 2.0 37.0 86-87 28.994107380399846 34.0 29.0 35.0 2.0 36.5 88-89 28.548566109001932 34.0 29.0 35.0 2.0 36.0 90-91 28.09223679227879 34.0 28.5 35.0 2.0 36.0 92-93 27.646354465039956 34.0 27.0 35.0 2.0 35.0 94-95 27.142988328850656 34.0 26.0 35.0 2.0 35.0 96-97 26.542542650185943 33.5 25.0 35.0 2.0 35.0 98-99 25.830052481332956 33.0 23.5 35.0 2.0 35.0 100-101 23.259461932827776 30.0 9.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2849530772448752 1101 2 0.36992607640790354 1101 3 0.28228180498420485 1101 4 0.2494334676823442 1101 5 0.15349795243005104 1101 6 0.12856883868643365 1101 7 0.14539792686342423 1101 8 0.17297851136552822 1101 9 0.26958830306653425 1101 10-11 0.24464623053857082 1101 12-13 0.3540748333146908 1101 14-15 0.20343271089034687 1101 16-17 0.4548104724912889 1101 18-19 0.38946161992490147 1101 20-21 0.5734291416244517 1101 22-23 0.3633598823459252 1101 24-25 0.5711644853289712 1101 26-27 0.3719767267264231 1101 28-29 0.3356103236907586 1101 30-31 0.41167601534223053 1101 32-33 0.43726932330888957 1101 34-35 0.24844415491865135 1101 36-37 0.37264661246779696 1101 38-39 0.2442746145451835 1101 40-41 0.18101878257327542 1101 42-43 0.13044373961602673 1101 44-45 0.2563674961261029 1101 46-47 0.32066406997834207 1101 48-49 0.3898522392609962 1101 50-51 0.3098361293115204 1101 52-53 0.32636483340026246 1101 54-55 0.2693848182530587 1101 56-57 0.2545552795419539 1101 58-59 0.2092009765903171 1101 60-61 0.07741832298514595 1101 62-63 0.1900487735268399 1101 64-65 0.06294640057041789 1101 66-67 0.12256272038880667 1101 68-69 0.10859570246132932 1101 70-71 0.12630874693163463 1101 72-73 0.09004842355091114 1101 74-75 0.1763431764756298 1101 76-77 0.15386559959558 1101 78-79 -0.06730301193107024 1101 80-81 -0.1106242761330627 1101 82-83 -0.05383457378290046 1101 84-85 -0.19767610982962225 1101 86-87 -0.1487132384118084 1101 88-89 -0.12820518220885901 1101 90-91 -0.06357018491161526 1101 92-93 -0.13346882276839622 1101 94-95 -0.03183093314608243 1101 96-97 -0.09027021396494916 1101 98-99 -0.050112049734639896 1101 100-101 0.24301602830245272 1102 1 0.13758530961586501 1102 2 0.10756107226623257 1102 3 0.13513756518241493 1102 4 0.1698829038369567 1102 5 0.14176743985654383 1102 6 0.25570036160572585 1102 7 0.1475295565635193 1102 8 0.13405306230951197 1102 9 0.2299905620359084 1102 10-11 0.19561775081539423 1102 12-13 0.2041121496481395 1102 14-15 0.4420110796041925 1102 16-17 0.39176580138044415 1102 18-19 0.5277911751159081 1102 20-21 0.5687154095839162 1102 22-23 0.4296654969947653 1102 24-25 0.4626102604679474 1102 26-27 0.338912547473619 1102 28-29 0.4018797752388892 1102 30-31 0.43944059184811834 1102 32-33 0.45898341441769475 1102 34-35 0.2909693986138038 1102 36-37 0.36507770396414685 1102 38-39 0.3824414357830008 1102 40-41 0.3374263287588235 1102 42-43 0.12550761874356908 1102 44-45 0.28047390448382004 1102 46-47 0.2631194647868469 1102 48-49 0.4044352231837891 1102 50-51 0.33882896987182676 1102 52-53 0.32631571422803773 1102 54-55 0.3248773810408849 1102 56-57 0.2850883264939057 1102 58-59 0.43413560537893403 1102 60-61 0.34142903295793303 1102 62-63 0.317043617010377 1102 64-65 0.3308857900746318 1102 66-67 0.27353768612640295 1102 68-69 0.1465713718132733 1102 70-71 0.1885455856274021 1102 72-73 0.16547198030861665 1102 74-75 0.22293150114737514 1102 76-77 0.17631719015980352 1102 78-79 0.1927509707510744 1102 80-81 -0.06469656873412077 1102 82-83 -0.049409007552743134 1102 84-85 0.04765408063601839 1102 86-87 0.08312427137999379 1102 88-89 0.1358627622776467 1102 90-91 0.026225356889941764 1102 92-93 0.07754614448406372 1102 94-95 -0.1029476639334419 1102 96-97 -0.1726200011294381 1102 98-99 -0.3170663506805518 1102 100-101 0.020297030117159665 1103 1 0.027234441813426713 1103 2 0.24126429966871754 1103 3 0.30002543630485334 1103 4 0.28953233833222924 1103 5 0.22560374758673873 1103 6 0.19548654005511423 1103 7 0.22643674756048426 1103 8 0.1589967836276287 1103 9 0.18402106415241093 1103 10-11 0.285395595277393 1103 12-13 0.335942101915613 1103 14-15 0.30959095345452425 1103 16-17 0.4458965054817412 1103 18-19 0.3417632644396136 1103 20-21 0.4543606224500394 1103 22-23 0.5015502289357343 1103 24-25 0.5524403830205529 1103 26-27 0.45300455746775015 1103 28-29 0.38874133425752433 1103 30-31 0.5116851690015665 1103 32-33 0.4279744639093721 1103 34-35 0.4892904260266633 1103 36-37 0.4779440215370414 1103 38-39 0.4505869668937308 1103 40-41 0.5173755593834173 1103 42-43 0.27982297405293366 1103 44-45 0.43807723275389776 1103 46-47 0.40312449013964624 1103 48-49 0.32804528465006655 1103 50-51 0.1493275475472231 1103 52-53 0.43606378929882084 1103 54-55 0.5485776880810604 1103 56-57 0.5225169710602984 1103 58-59 0.47773428492033787 1103 60-61 0.28038597272885823 1103 62-63 0.16371405731393196 1103 64-65 0.22138052385435003 1103 66-67 0.3412856541449685 1103 68-69 0.2390847332334829 1103 70-71 0.39638746561964666 1103 72-73 0.28122496369582706 1103 74-75 0.14383530474593798 1103 76-77 0.12029643201378093 1103 78-79 0.2110299981955457 1103 80-81 0.1233090345897132 1103 82-83 0.0840570964149272 1103 84-85 0.06878566140067832 1103 86-87 0.14513981502914675 1103 88-89 0.21447888772510026 1103 90-91 0.27490904448938736 1103 92-93 0.17884721719720176 1103 94-95 0.06175702586889287 1103 96-97 -0.026286830778023074 1103 98-99 0.00934820154304461 1103 100-101 0.33878403284088776 1104 1 0.21460435201847972 1104 2 0.3222684352011349 1104 3 0.34712264554460504 1104 4 0.3350242777391159 1104 5 0.2757942820170669 1104 6 0.3199837427438439 1104 7 0.1757372911595354 1104 8 0.2524307968861308 1104 9 0.3397132887180163 1104 10-11 0.37479220601949237 1104 12-13 0.38224628620167067 1104 14-15 0.48001470949898817 1104 16-17 0.45607480954258506 1104 18-19 0.45658919011589205 1104 20-21 0.4490506443039095 1104 22-23 0.3936678397203295 1104 24-25 0.5114204600789165 1104 26-27 0.40125423133810045 1104 28-29 0.47395003151743964 1104 30-31 0.5959309750649382 1104 32-33 0.6196564050027362 1104 34-35 0.4922155091934499 1104 36-37 0.4390571042696223 1104 38-39 0.503473298900893 1104 40-41 0.5797015818360762 1104 42-43 0.5363385964685605 1104 44-45 0.5117741421086706 1104 46-47 0.5957044890189351 1104 48-49 0.7224365314087393 1104 50-51 0.6036320393520924 1104 52-53 0.6319815583364985 1104 54-55 0.54441481464265 1104 56-57 0.5244588602923486 1104 58-59 0.38527304352430747 1104 60-61 0.4231889362789403 1104 62-63 0.325705274078679 1104 64-65 0.3673281108468416 1104 66-67 0.557192687094691 1104 68-69 0.3465552475668119 1104 70-71 0.46307113244575504 1104 72-73 0.5060339352322032 1104 74-75 0.37578908055444415 1104 76-77 0.3524585139161083 1104 78-79 0.4282169157607463 1104 80-81 0.3535573803902814 1104 82-83 0.4351542428515387 1104 84-85 0.4546213342862053 1104 86-87 0.347236519407474 1104 88-89 0.2653714908420284 1104 90-91 0.28409874055197903 1104 92-93 0.2239505621951352 1104 94-95 0.32773940904761645 1104 96-97 0.3940712120705001 1104 98-99 0.43742412728699875 1104 100-101 1.0686994137351427 1105 1 0.0508368208269836 1105 2 -0.00782666955353406 1105 3 0.0184593539404041 1105 4 0.007270697360780787 1105 5 0.0786000010492316 1105 6 0.01735582972337113 1105 7 0.09621807237176938 1105 8 0.10310418350143635 1105 9 0.03467127212990562 1105 10-11 0.037510460622613095 1105 12-13 0.11116716889990386 1105 14-15 0.010576662342288046 1105 16-17 0.078383883996608 1105 18-19 0.1884866000793295 1105 20-21 0.1210777655088151 1105 22-23 0.12368352358931389 1105 24-25 0.05350533506971544 1105 26-27 -0.132597848107757 1105 28-29 0.0812859407617168 1105 30-31 0.09756946981934078 1105 32-33 0.06632890922156065 1105 34-35 0.10203302845750528 1105 36-37 0.045276620684091995 1105 38-39 0.048794191276357424 1105 40-41 -0.029765658284240715 1105 42-43 -0.10016595325819821 1105 44-45 -0.23148399241880924 1105 46-47 -0.1669494588598397 1105 48-49 -0.02630172227348737 1105 50-51 -0.10206116086216355 1105 52-53 -0.043553557115139085 1105 54-55 0.04697537729527568 1105 56-57 -0.14800035423431268 1105 58-59 -0.15874181361825634 1105 60-61 -0.015364974061562009 1105 62-63 -0.2438120638934791 1105 64-65 -0.05844379746820749 1105 66-67 -0.19629326024715965 1105 68-69 -0.32371358419696605 1105 70-71 -0.4585024988836821 1105 72-73 -0.16149717821843623 1105 74-75 -0.19591590870565767 1105 76-77 -0.1676176968361709 1105 78-79 -0.18973675686339675 1105 80-81 -0.3674687593046322 1105 82-83 -0.2952662195897702 1105 84-85 -0.2461128081360151 1105 86-87 -0.23122509829685356 1105 88-89 -0.1865753614192549 1105 90-91 -0.19122420433179954 1105 92-93 -0.24580732767023505 1105 94-95 0.0061696277971350355 1105 96-97 0.04504490484512402 1105 98-99 -0.024463602849522204 1105 100-101 0.062627865220378 1106 1 0.09318132977500682 1106 2 0.16353496892699937 1106 3 0.1671574429042444 1106 4 0.18325788450274416 1106 5 0.16626227976923502 1106 6 0.13644208656146617 1106 7 0.26570377288332026 1106 8 0.22110295103036037 1106 9 0.14771998398583008 1106 10-11 0.23141625928807485 1106 12-13 0.219058438123227 1106 14-15 0.19118561601266748 1106 16-17 0.3253298115975056 1106 18-19 0.26755612476101476 1106 20-21 0.3291593768790193 1106 22-23 0.23357049832667798 1106 24-25 0.16042358754273067 1106 26-27 0.29209219348562243 1106 28-29 0.1997162496661673 1106 30-31 0.24588253764357404 1106 32-33 0.3400398574376098 1106 34-35 0.16642522959839567 1106 36-37 0.22098556890820475 1106 38-39 0.1649584982282235 1106 40-41 0.38865461536064316 1106 42-43 0.3137471524882187 1106 44-45 0.1502261418822073 1106 46-47 0.12650510612426302 1106 48-49 0.08613630117371684 1106 50-51 0.005183111475616897 1106 52-53 -0.12221700678352221 1106 54-55 -0.12816826811614845 1106 56-57 -0.028451290387870642 1106 58-59 0.13295431166212524 1106 60-61 0.08871163581596164 1106 62-63 -0.07013828143772827 1106 64-65 -0.14583111511896618 1106 66-67 -0.04268079373164113 1106 68-69 0.022589103437177016 1106 70-71 0.12714844150586657 1106 72-73 -0.018325920210401137 1106 74-75 -0.041782642475769904 1106 76-77 -0.007676335026236103 1106 78-79 0.117148414552954 1106 80-81 0.17115602183593737 1106 82-83 0.22621137908860334 1106 84-85 0.31440207632234163 1106 86-87 0.38375212317678375 1106 88-89 0.5958014620731333 1106 90-91 0.5238562441585763 1106 92-93 0.5139594004518919 1106 94-95 0.5162680489835338 1106 96-97 0.7865802390444827 1106 98-99 0.8908931430637601 1106 100-101 1.3820094562174852 1107 1 0.06983378243463179 1107 2 0.08740924732329347 1107 3 0.06727628944847552 1107 4 0.15540080961454805 1107 5 -0.002431419282366676 1107 6 -0.08275395968460941 1107 7 -0.043214475760699145 1107 8 0.009074867958709376 1107 9 0.013363420563997863 1107 10-11 0.101839576999879 1107 12-13 0.09684498814385023 1107 14-15 0.06829591036354543 1107 16-17 0.04993664661193975 1107 18-19 0.05932934691980307 1107 20-21 0.006879565130354592 1107 22-23 -0.058242124583841814 1107 24-25 0.1712731668715506 1107 26-27 0.12328623946208239 1107 28-29 0.02413088830923016 1107 30-31 0.07253310699682203 1107 32-33 0.06423162163972762 1107 34-35 0.04069594398605858 1107 36-37 -0.10676301762080698 1107 38-39 0.015642782730395766 1107 40-41 -0.04165041088670307 1107 42-43 0.10985941340743466 1107 44-45 0.36304397461288573 1107 46-47 0.4296587026447085 1107 48-49 0.4577954147906169 1107 50-51 0.4048368309065893 1107 52-53 0.41965161971122455 1107 54-55 0.3038411503981493 1107 56-57 0.5204202621702478 1107 58-59 0.5174994535246569 1107 60-61 0.42417682240205323 1107 62-63 0.36812148400141353 1107 64-65 0.40150950760124715 1107 66-67 0.4032036366772118 1107 68-69 0.39941789304608477 1107 70-71 0.4790556361326921 1107 72-73 0.36007567802285934 1107 74-75 0.4717171949651551 1107 76-77 0.5149838054882458 1107 78-79 0.46643806112573216 1107 80-81 0.35517611770614366 1107 82-83 0.4655858140966913 1107 84-85 0.420483272598549 1107 86-87 0.6329499833442611 1107 88-89 0.638129764004816 1107 90-91 0.7816474330601544 1107 92-93 0.6201972085211658 1107 94-95 0.33257951415624376 1107 96-97 0.3697019482143986 1107 98-99 0.5104471662026562 1107 100-101 0.8281848079001719 1108 1 0.17144495748125976 1108 2 0.17507145614110087 1108 3 0.020713595161662823 1108 4 0.10352485333762473 1108 5 0.1863437084784323 1108 6 0.11803222671608893 1108 7 0.12415107225487532 1108 8 0.22126141857138748 1108 9 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-1.734321280373976 2316 90-91 -1.917945217495003 2316 92-93 -2.2092429511124045 2316 94-95 -2.400979327330706 2316 96-97 -2.656152343263468 2316 98-99 -3.3516365073033363 2316 100-101 -3.8729539225013987 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6496.0 3 1549.0 4 1549.0 5 1711.0 6 2015.0 7 2601.0 8 3071.0 9 3866.0 10 4971.0 11 6621.0 12 9174.0 13 13671.0 14 13286.0 15 13224.0 16 16865.0 17 22831.0 18 19123.0 19 12640.0 20 12759.0 21 13283.0 22 14475.0 23 16417.0 24 18218.0 25 21223.0 26 24454.0 27 28521.0 28 33176.0 29 39640.0 30 47360.0 31 57364.0 32 70101.0 33 89341.0 34 119503.0 35 168160.0 36 252349.0 37 396596.0 38 405197.0 39 76357.0 40 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.198712265177555 20.159710668655137 14.67077020155913 21.970806864608182 2 15.048705266446744 21.798540421216615 40.17320775455286 22.97954655778378 3 18.89291889102375 26.300769037143727 31.341057720061926 23.465254351770593 4 12.061331886982453 17.637413053705497 39.09905365106396 31.202201408248087 5 12.486725685221952 38.49359309273916 35.06591593379489 13.953765288244 6 29.09519200494443 37.81908504658729 18.375013119074552 14.710709829393723 7 26.058641102419294 34.523979602249696 22.28469770258043 17.132681592750576 8 25.0859236777132 34.6553893206332 21.158386748632484 19.100300253021114 9 25.9015980787678 17.15385893793802 22.08270200977138 34.8618409735228 10-11 23.917221802042437 27.22291235368248 28.3373052673214 20.52256057695368 12-13 25.475354088927137 25.279667931232375 28.6089566628294 20.63602131701109 14-15 22.34931432953588 26.3931128729539 27.870154648844775 23.387418148665446 16-17 20.46208605651255 29.58123375203479 28.64197380888748 21.314706382565173 18-19 20.31062633354935 28.301048712563727 31.175574247824102 20.21275070606282 20-21 21.566246118488788 27.14981472990222 30.759623195442032 20.52431595616696 22-23 21.095685669325785 27.195387354108064 30.762289554106847 20.946637422459307 24-25 21.13237182120327 27.325292267247157 30.90940335140564 20.632932560143928 26-27 20.860683680699456 27.21149814477444 31.387329532541496 20.54048864198461 28-29 20.403035954989544 27.835598779123778 31.26606847814833 20.49529678773835 30-31 20.765831569159776 27.493256608086625 30.9331337919577 20.807778030795895 32-33 20.58513187350486 27.594019349677424 30.945850842429312 20.874997934388396 34-35 21.211007645132135 27.502396033586628 30.709364842139586 20.577231479141652 36-37 21.146675431446447 27.12844228016462 30.980729042791307 20.744153245597627 38-39 20.83347066709539 27.61473106631304 30.94545933077319 20.60633893581838 40-41 20.86326194559511 27.63833260644767 30.86287389359419 20.635531554363034 42-43 20.603343723268377 27.81460801431155 30.29603502892386 21.286013233496213 44-45 20.880851042009784 27.668168431775825 30.130661133959226 21.32031939225517 46-47 21.20506825077377 27.96310217339449 29.837511178995523 20.994318396836213 48-49 21.1161646933276 28.01158972943867 29.706605974840077 21.16563960239365 50-51 20.730075762440904 28.286467832278994 29.842177656782738 21.141278748497363 52-53 20.99329358583155 28.65824832290808 29.186014596657927 21.16244349460244 54-55 20.63817423130733 28.165319282447705 29.472554625829243 21.723951860415717 56-57 21.082651751316114 28.393673083264925 29.122402586069484 21.401272579349477 58-59 21.277691018539358 28.039427802346456 29.246704708382374 21.436176470731816 60-61 21.053531663085334 28.171675654154445 29.65910000214685 21.115692680613375 62-63 20.903116998275387 28.08899529100643 29.73333444010633 21.274553270611857 64-65 20.74915792023883 28.207034394366858 29.55072309447705 21.49308459091726 66-67 20.765795593576954 28.192416884033953 29.033829180700177 22.007958341688916 68-69 20.718147431115618 28.13081181055165 29.332120837296415 21.81891992103632 70-71 20.99404277769229 28.04606039289823 29.09181059012894 21.86808623928054 72-73 20.90952519582424 27.62118242866996 29.454842705608126 22.014449669897676 74-75 20.551841061724637 28.459190728519197 28.973548467611533 22.015419742144633 76-77 20.776670485701086 27.865276967901554 29.110736370393596 22.247316176003768 78-79 20.69275808395162 27.822571982665178 29.23869164682745 22.245978286555747 80-81 20.74398707765151 28.021303224801652 28.998925660123387 22.23578403742345 82-83 20.67192427173657 27.78162986082695 29.234763085498987 22.311682781937485 84-85 20.554831179739615 27.636296600154314 29.14439821461709 22.664474005488977 86-87 20.56180548875378 27.678591047656344 29.227616333151524 22.53198713043835 88-89 20.72664478993478 27.397005057661445 29.002793641894716 22.87355651050906 90-91 20.605784867442434 27.241856536127518 29.209113929491327 22.94324466693872 92-93 20.783164677749504 27.418701316590628 29.153493311293683 22.64464069436619 94-95 20.63543739838431 27.109313999293192 29.15245949965209 23.102789102670407 96-97 20.3752408356671 26.894807962786082 29.42179657729442 23.3081546242524 98-99 20.479779329155946 27.005141626758732 28.9300033411293 23.585075702956022 100-101 20.554028619790063 26.369773704212683 29.42619284124328 23.65000483475397 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3647.0 1 2231.5 2 941.5 3 1041.5 4 884.5 5 899.5 6 1519.5 7 2760.5 8 4170.5 9 4770.5 10 4241.5 11 3773.0 12 3892.0 13 4145.5 14 4422.0 15 4652.5 16 4826.0 17 4991.5 18 5177.5 19 5495.0 20 5971.5 21 6672.0 22 7646.0 23 9156.5 24 10882.0 25 12868.0 26 15998.5 27 19404.5 28 21743.0 29 24449.0 30 28726.0 31 33230.5 32 37919.0 33 43841.0 34 50521.0 35 55248.0 36 60381.5 37 66296.5 38 68578.5 39 68458.5 40 68156.0 41 68226.5 42 67735.0 43 68617.5 44 70278.0 45 70176.5 46 70643.5 47 76163.5 48 91991.0 49 92024.0 50 75121.0 51 65484.0 52 59417.5 53 56008.5 54 52463.0 55 47813.5 56 42939.5 57 37661.0 58 32706.0 59 27903.5 60 23397.0 61 19531.0 62 15970.5 63 12090.5 64 8752.0 65 6414.0 66 4686.5 67 3664.5 68 2763.5 69 1875.5 70 1242.0 71 858.5 72 637.0 73 472.5 74 371.0 75 278.0 76 190.5 77 122.0 78 71.0 79 47.5 80 34.0 81 23.0 82 18.5 83 14.5 84 12.0 85 12.5 86 12.5 87 10.0 88 6.0 89 2.5 90 4.5 91 5.0 92 1.5 93 2.0 94 2.0 95 1.5 96 2.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28842886133470563 2 0.08685393585722748 3 0.09136898115332706 4 0.10622493664371924 5 0.10850673372884484 6 0.08292147705094718 7 0.09253415413296566 8 0.08976686830632398 9 0.09389352260921069 10-11 0.08731515016166776 12-13 0.09190301876899475 14-15 0.10127295148025517 16-17 0.08884443969744342 18-19 0.1003262484342988 20-21 0.10236530114866636 22-23 0.11615318140772316 24-25 0.1297711406072493 26-27 0.13363077610230217 28-29 0.12472205769548203 30-31 0.11629882803017798 32-33 0.110594335317364 34-35 0.3860363728165144 36-37 0.5918593247822583 38-39 0.5752798842594841 40-41 0.5381885444076552 42-43 0.5653030906213284 44-45 0.5863975764402024 46-47 0.5755226286302421 48-49 0.5960588023963724 50-51 0.5888735690219343 52-53 0.5917136781598035 54-55 0.581858256707027 56-57 0.5544524172484441 58-59 0.5499130975152686 60-61 0.49830564429210883 62-63 0.5720999330025537 64-65 0.6053073629222538 66-67 0.6187796754993252 68-69 0.6196292807969783 70-71 0.6158424686131528 72-73 0.6086086863645632 74-75 0.6053801862334812 76-77 0.5894804299488295 78-79 0.5654972861179349 80-81 0.5605210265173951 82-83 0.5741389857169212 84-85 0.5845284447853655 86-87 0.575158512074105 88-89 0.5871743584266281 90-91 0.6013506296788977 92-93 0.6097981337812776 94-95 0.6090213517948518 96-97 0.6059627727233006 98-99 0.6101622503374147 100-101 0.5877326704793716 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2059780.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.3676225518745 #Duplication Level Percentage of deduplicated Percentage of total 1 71.32973665548501 33.787200426216216 2 14.552760235113855 13.786593078096027 3 5.530362149680279 7.858803206436861 4 2.741061939827689 5.193503494282677 5 1.585863682026783 3.755929615448529 6 0.9839217768253636 2.796362120714012 7 0.6458329111224949 2.1414098695940047 8 0.4522972932932707 1.7139397975960102 9 0.3121905899478446 1.3308953426006875 >10 1.6872995863457332 14.355861700066427 >50 0.11286418476375779 3.6883987683881383 >100 0.06076416393186679 5.041906158616161 >500 0.0023679821964994467 0.8130549269068867 >1k 0.002265026448825557 2.076479470535528 >5k 3.0886724302166686E-4 1.1085871280258723 >10k+ 1.0295574767388895E-4 0.5510748964759967 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11300 0.5486022779131752 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9264 0.4497567701405004 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7670 0.3723698647428366 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5798 0.28148637233102564 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4474 0.21720766295429608 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3410 0.16555166085698472 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2700 0.13108196020934273 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2567 0.12462495994717883 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2247 0.10908932021866412 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 4.854887415160842E-5 0.0 16-17 0.0 0.0 0.0 1.4564662245482527E-4 0.0 18-19 0.0 0.0 0.0 2.9129324490965053E-4 0.0 20-21 0.0 0.0 0.0 4.126654302886716E-4 0.0 22-23 0.0 0.0 0.0 7.282331122741264E-4 0.0 24-25 0.0 0.0 0.0 0.0013350940391692317 0.0 26-27 0.0 0.0 0.0 0.0027915602637174847 0.0 28-29 0.0 0.0 0.0 0.008690248473137908 0.0 30-31 0.0 0.0 0.0 0.022648049791725328 0.0 32-33 0.0 0.0 0.0 0.04968977269417122 0.0 34-35 0.0 0.0 0.0 0.09488877452931867 0.0 36-37 0.0 0.0 0.0 0.16691102933322977 0.0 38-39 0.0 0.0 0.0 0.2737913757779957 0.0 40-41 0.0 0.0 0.0 0.3879540533455029 0.0 42-43 0.0 0.0 0.0 0.5052724077328647 0.0 44-45 0.0 0.0 0.0 0.6554098010467138 0.0 46-47 0.0 0.0 0.0 0.8312052743496878 0.0 48-49 0.0 0.0 0.0 1.0176329510918642 0.0 50-51 0.0 0.0 0.0 1.213794677101438 0.0 52-53 0.0 0.0 0.0 1.406703628542854 0.0 54-55 0.0 4.854887415160842E-5 0.0 1.6296400586470399 0.0 56-57 0.0 4.854887415160842E-5 0.0 1.898309528202041 0.0 58-59 0.0 4.854887415160842E-5 0.0 2.1871025060928835 0.0 60-61 0.0 4.854887415160842E-5 0.0 2.503349872316461 0.0 62-63 0.0 4.854887415160842E-5 0.0 2.8216605656914817 0.0 64-65 0.0 9.709774830321684E-5 0.0 3.152399770849314 0.0 66-67 0.0 9.709774830321684E-5 0.0 3.538508966977056 0.0 68-69 0.0 9.709774830321684E-5 0.0 3.9659089805707404 0.0 70-71 0.0 9.709774830321684E-5 0.0 4.436881608715494 0.0 72-73 0.0 9.709774830321684E-5 0.0 4.912150812222665 0.0 74-75 0.0 9.709774830321684E-5 0.0 5.410237986581091 0.0 76-77 0.0 9.709774830321684E-5 0.0 5.9451009331093605 0.0 78-79 0.0 9.709774830321684E-5 0.0 6.5563555331151875 0.0 80-81 0.0 9.709774830321684E-5 0.0 7.204725747409917 0.0 82-83 0.0 9.709774830321684E-5 0.0 7.861422093621648 0.0 84-85 0.0 9.709774830321684E-5 0.0 8.543048286710231 0.0 86-87 0.0 9.709774830321684E-5 0.0 9.263634951305479 0.0 88-89 0.0 1.4564662245482527E-4 0.0 10.034154132965657 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 90 9.142867E-4 26.29267 2 GTACATG 9750 0.0 24.848782 1 TACATGG 9820 0.0 24.048958 2 ACATGGG 9835 0.0 23.049862 3 GAGTACT 8885 0.0 22.718206 12-13 ATCACGC 105 0.0022439365 22.536573 3 CTACACG 395 0.0 21.566645 4 TAGGACG 245 7.850758E-9 21.24877 4 AGTACTT 9350 0.0 20.905035 12-13 GTACTTT 9750 0.0 20.630135 14-15 CATGGGG 3370 0.0 19.941858 4 CATGGGA 6415 0.0 19.402884 4 CCCTATA 470 0.0 19.132113 2 GTAGGAC 595 0.0 19.089804 3 GTATCAA 22935 0.0 18.729158 1 GGTATCA 18515 0.0 17.720304 1 AGAGTAC 17125 0.0 17.32783 10-11 ACTTTTT 11935 0.0 17.171762 16-17 AGTACAT 7440 0.0 16.793383 2 TACTTTT 11655 0.0 16.669348 14-15 >>END_MODULE