FastQCFastQC Report
Fri 27 May 2016
SRR938978_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938978_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2059780
Sequences flagged as poor quality0
Sequence length101
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110610.5369990969909408No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93930.45601957490605793No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT82480.4004311140024663No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62950.30561516278437506No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT41110.19958442163726223No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT31930.1550165551660857No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC26590.12909145636912678No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA25460.12360543358999505No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT23310.11316742564739923No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA22260.10806979386148036No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC22200.1077785006165707No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC20730.10064181611628427No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20720.10059326724213265No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG97450.024.2957531
TACATGG97150.023.98132
ACATGGG97000.023.0400413
GAGTACT93550.022.5979812-13
AGTACTT99150.020.65160212-13
GTACTTT104550.020.2657714-15
CATGGGA64200.020.0294174
CCTACGC1708.0227175E-519.5381373
GTCCTAC4300.018.8337291
CATGGGG34250.018.7028264
GGTATCA186450.018.1917081
GTATCAA229350.018.1538431
GTGTAGC10500.017.694211
ACTTTTT122650.017.33204516-17
AGAGTAC178500.017.30646910-11
ACGCAGT2504.6099813E-617.0819156
TACGCCC1700.001800912716.7469755
TACTTTT121850.016.7264314-15
CTAGGGG4004.3837645E-1016.6074183
GTACTAG3803.767127E-916.29731
Produced by FastQC (version 0.11.5)