FastQCFastQC Report
Fri 27 May 2016
SRR938977_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938977_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3110129
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144930.4659935327441402No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120360.3869935941563839No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT79670.2561630080295705No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75540.24288381607322396No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT43220.13896529693784404No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT32610.10485095634296841No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG132100.025.7628251
TACATGG132800.025.1421572
ACATGGG137050.023.2566683
GAGTACT108700.022.10224212-13
GGTATCA187850.021.7049671
GTATCAA241900.021.2112851
AGTACTT114000.020.34796712-13
GTACTTT119200.019.8779514-15
CATGGGG68700.019.6471524
CATGGGA75050.018.7420564
ATGGGGA39300.018.4378535
AGAGTAC201150.018.32151610-11
ATCAACG278900.018.2545623
CAACGCA279800.018.212775
TCAACGC279900.018.2062644
TATCAAC282300.018.0514832
AACGCAG284600.017.9646456
TACCTGG19950.017.3297162
ACTTTTT142700.017.07012216-17
TACTTTT135200.016.96527314-15
Produced by FastQC (version 0.11.5)