FastQCFastQC Report
Fri 27 May 2016
SRR938977_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938977_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3110129
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142950.4596272373268119No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126760.40757151873764724No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT85310.2742973040668088No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84900.27297903077332164No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT42020.13510693607885718No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG135400.023.4004361
TACATGG136150.022.5161362
GAGTACT120200.021.45897512-13
ACATGGG136600.021.2955443
GTATCAA254700.021.2879711
GGTATCA201450.020.623871
GTATAGG8550.020.6184771
AGTACTT126650.019.99139612-13
GTACTTT130950.019.5705114-15
CATGGGG65050.018.8943274
AGAGTAC221050.018.36721810-11
ATCAACG296750.018.1343213
TCAACGC298850.017.9910144
TATCAAC301050.017.9383542
CAACGCA299050.017.9313775
AACGCAG302900.017.7817946
CATGGGA77600.017.7343484
GCACCGT8350.017.6179036
TCGCGCT5050.017.38550460-61
CGCGGAA4750.017.08209282-83
Produced by FastQC (version 0.11.5)