FastQCFastQC Report
Fri 27 May 2016
SRR938976_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938976_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3118863
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145160.46542602223951485No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119770.3840181502040968No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT78040.25021939084852396No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77620.24887274625400346No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT44250.14187862692269587No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT32230.10333894114618052No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG131200.025.079651
TACATGG132250.024.4401932
ACATGGG132100.023.643993
GAGTACT113350.022.46140712-13
GGTATCA190550.021.8215941
GTATCAA242600.021.3416351
GTATAGG9400.021.1844541
CATGGGG65500.020.8080234
GTACTTT122950.020.63095914-15
CATGGGA71550.020.0406884
AGTACTT119600.019.78403912-13
TGCACCG7800.019.4148925
ATCAACG282000.018.2246993
TATCAAC285000.018.1158852
TCAACGC283450.018.1147754
CAACGCA283250.018.1108575
AACGCAG287600.017.8516646
AGAGTAC205850.017.80476610-11
ATGGGGA38350.017.5227645
CGTACAC2453.9016722E-617.3842533
Produced by FastQC (version 0.11.5)