FastQCFastQC Report
Fri 27 May 2016
SRR938976_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938976_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3118863
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141990.45526206184753865No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129830.41627349453951645No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT84450.27077175239822976No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81890.2625636329649619No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT42400.1359469781134984No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG129600.024.3725851
TACATGG130450.023.28012
ACATGGG131150.022.1065963
GAGTACT119150.021.92432212-13
GGTATCA199550.021.6067731
GTATCAA252150.021.1050931
GTACTTT131150.019.8459214-15
AGTACTT124850.019.7641312-13
CATGGGG63800.019.1144334
AGAGTAC210800.018.58272410-11
CATGGGA75500.018.352064
ATCAACG295100.017.7520623
GTATAGG7800.017.7131771
TCAACGC296300.017.6801684
TATCAAC298600.017.6393322
CAACGCA297000.017.622525
AACGCAG300000.017.477936
AGTACAT101100.017.1313132
ACTTTTT156150.016.74410816-17
TACTTTT150400.016.35926614-15
Produced by FastQC (version 0.11.5)