##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938976_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3118863 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.144913707335014 33.0 31.0 34.0 30.0 34.0 2 32.37321902244504 34.0 31.0 34.0 30.0 34.0 3 32.46212610172361 34.0 31.0 34.0 30.0 34.0 4 36.04561341745373 37.0 35.0 37.0 35.0 37.0 5 35.963004146062204 37.0 35.0 37.0 35.0 37.0 6 36.0037350149718 37.0 35.0 37.0 35.0 37.0 7 35.94200386486999 37.0 35.0 37.0 35.0 37.0 8 35.909475985319006 37.0 35.0 37.0 35.0 37.0 9 37.58335265127067 39.0 37.0 39.0 35.0 39.0 10-11 37.500271893956224 39.0 37.0 39.0 34.5 39.0 12-13 37.42397550004601 39.0 37.0 39.0 34.0 39.0 14-15 38.664287915179344 40.0 38.0 41.0 34.0 41.0 16-17 38.65517049001511 40.0 38.0 41.0 34.0 41.0 18-19 38.76300930819981 40.0 38.0 41.0 34.0 41.0 20-21 38.7657739374894 40.0 38.0 41.0 34.0 41.0 22-23 38.703772656894515 40.0 38.0 41.0 34.0 41.0 24-25 38.636632003393544 40.0 38.0 41.0 34.0 41.0 26-27 38.480202560997384 40.0 38.0 41.0 34.0 41.0 28-29 38.29288253443643 40.0 38.0 41.0 34.0 41.0 30-31 38.11424355606514 40.0 37.5 41.0 33.0 41.0 32-33 37.95080194288752 40.0 37.0 41.0 33.0 41.0 34-35 37.91482440876691 40.0 37.0 41.0 33.0 41.0 36-37 37.80821039590389 40.0 37.0 41.0 33.0 41.0 38-39 37.68210258033136 40.0 37.0 41.0 33.0 41.0 40-41 37.52302970665912 40.0 37.0 41.0 32.5 41.0 42-43 37.337223853692834 40.0 37.0 41.0 32.0 41.0 44-45 37.07368165898919 40.0 36.0 41.0 31.0 41.0 46-47 36.743579791738206 40.0 36.0 41.0 30.5 41.0 48-49 36.43313428643708 39.0 35.5 41.0 30.0 41.0 50-51 36.15135483668247 39.0 35.0 41.0 29.5 41.0 52-53 35.9158191623037 39.0 35.0 40.5 29.0 41.0 54-55 35.656657730717896 39.0 35.0 40.0 28.0 41.0 56-57 35.369012040605824 38.0 35.0 40.0 28.0 41.0 58-59 35.052303355421515 38.0 34.0 40.0 27.0 41.0 60-61 34.63304864625346 37.5 34.0 40.0 26.0 41.0 62-63 34.30880243858098 37.0 34.0 40.0 26.0 41.0 64-65 34.044210342038106 37.0 33.5 39.5 26.0 41.0 66-67 33.58350174406506 36.0 33.0 39.0 25.0 40.5 68-69 33.16382604814639 35.5 32.5 39.0 24.0 40.0 70-71 32.69319893179021 35.0 32.0 38.0 23.0 40.0 72-73 32.17255967960119 35.0 31.5 37.0 22.0 39.5 74-75 31.703519519773714 35.0 31.0 37.0 20.5 39.0 76-77 29.743642635152618 33.0 28.5 35.0 18.0 37.0 78-79 30.729763057883595 34.0 30.5 36.0 18.0 38.0 80-81 30.73073440545481 34.0 31.0 35.5 18.0 37.0 82-83 30.408511851915264 34.0 30.5 35.0 17.5 37.0 84-85 30.006768973180293 34.0 30.0 35.0 10.5 36.0 86-87 29.421910965630744 34.0 29.5 35.0 6.0 36.0 88-89 28.8556568531545 34.0 29.0 35.0 2.0 35.5 90-91 28.386289811383186 33.5 28.5 35.0 2.0 35.0 92-93 28.014966351519767 33.0 27.5 35.0 2.0 35.0 94-95 27.603640974290954 33.0 27.0 35.0 2.0 35.0 96-97 26.962420600071244 33.0 26.0 35.0 2.0 35.0 98-99 26.02975940270541 32.5 24.5 34.0 2.0 35.0 100-101 21.646906100075572 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.009964962776216169 1101 2 0.033671559682879604 1101 3 0.12159875326076275 1101 4 0.041355343949319945 1101 5 0.061510658968423115 1101 6 0.03755227367981462 1101 7 0.0697986371904733 1101 8 0.12775156247524677 1101 9 0.13180854764869565 1101 10-11 0.21612041410956095 1101 12-13 0.15320300529967312 1101 14-15 0.20089335678071762 1101 16-17 0.13630501535678974 1101 18-19 0.12744251903971104 1101 20-21 0.1433399015788055 1101 22-23 0.11802579771757848 1101 24-25 0.06813275812504571 1101 26-27 0.095018010296684 1101 28-29 0.17253562102514763 1101 30-31 -0.0018798490739087015 1101 32-33 0.18726576932920125 1101 34-35 0.17459312800932025 1101 36-37 0.1481034191861852 1101 38-39 0.3088599745643492 1101 40-41 0.310553927386799 1101 42-43 0.22233274679444293 1101 44-45 0.1810607900739356 1101 46-47 0.276362576633872 1101 48-49 0.08286635769027129 1101 50-51 0.14447688158909244 1101 52-53 0.20709407842194594 1101 54-55 0.23042327890538417 1101 56-57 0.10371948012202381 1101 58-59 0.15413079993414414 1101 60-61 0.29817638805626245 1101 62-63 0.01045781949832758 1101 64-65 0.11611659834304788 1101 66-67 0.1553495639870519 1101 68-69 0.14562729575529687 1101 70-71 0.3091360302215662 1101 72-73 0.17903064827454074 1101 74-75 0.1486684967510321 1101 76-77 -0.10550372877353098 1101 78-79 0.12588265938157406 1101 80-81 0.260748755578291 1101 82-83 0.21138139288245839 1101 84-85 0.14952301297576653 1101 86-87 0.3424692687376556 1101 88-89 0.5151943409538937 1101 90-91 0.46153698211997707 1101 92-93 0.42892138725812146 1101 94-95 0.5998310212860893 1101 96-97 0.48461294669074917 1101 98-99 0.40261041653773333 1101 100-101 0.7206836061852187 1102 1 0.048106163749274344 1102 2 0.05219432320897255 1102 3 0.10965799581700963 1102 4 0.06871721358300675 1102 5 0.0526549319248204 1102 6 0.028023167881556788 1102 7 0.06515239303723064 1102 8 0.07778868256566085 1102 9 0.1395483635164254 1102 10-11 0.1569017504605128 1102 12-13 0.15109601310273035 1102 14-15 0.14185948562159467 1102 16-17 0.1681914154144053 1102 18-19 0.09536913345200304 1102 20-21 0.16095768976621372 1102 22-23 0.1420395298425774 1102 24-25 0.14755370779916177 1102 26-27 0.17476231797236608 1102 28-29 0.2175967098903655 1102 30-31 0.12298747199594118 1102 32-33 0.13801464637720784 1102 34-35 0.24035097574584796 1102 36-37 0.22513937996631483 1102 38-39 0.3002597944118719 1102 40-41 0.24307396600276832 1102 42-43 0.025140230647899386 1102 44-45 0.10522494166979612 1102 46-47 0.22738748818510146 1102 48-49 0.17140279424469185 1102 50-51 0.2253428138874014 1102 52-53 0.1754500093028568 1102 54-55 0.13761572348956008 1102 56-57 0.07529993030007631 1102 58-59 0.028240656472377168 1102 60-61 -0.07009057464774315 1102 62-63 -0.005676896419863908 1102 64-65 -0.004245006279049335 1102 66-67 0.05150322546383279 1102 68-69 0.14119377649831932 1102 70-71 0.11398159864141633 1102 72-73 -0.02335810341467237 1102 74-75 0.132612268586211 1102 76-77 -0.0029133674389960618 1102 78-79 0.016753979633310934 1102 80-81 0.2820873249681597 1102 82-83 0.3058535812087442 1102 84-85 0.2562201305822782 1102 86-87 0.3174564336815173 1102 88-89 0.48450430201934935 1102 90-91 0.5061676002849076 1102 92-93 0.5442501955416255 1102 94-95 0.4665009313437416 1102 96-97 0.32032549775972896 1102 98-99 0.5147563732872911 1102 100-101 0.7873559516716888 1103 1 -0.01861147852571321 1103 2 -0.00849169650945214 1103 3 0.039930435397657504 1103 4 0.03761472609118499 1103 5 -5.129222083297691E-4 1103 6 0.04462117953817568 1103 7 0.02914829141602837 1103 8 0.08954106486504543 1103 9 0.06980312369534403 1103 10-11 0.06830584854584032 1103 12-13 0.011136292673015191 1103 14-15 0.05828600708144194 1103 16-17 -0.04881661103397761 1103 18-19 0.012454949267997506 1103 20-21 0.06313829664113513 1103 22-23 0.03599479506306835 1103 24-25 0.07368180095782151 1103 26-27 0.036586743833794344 1103 28-29 0.11870255646851291 1103 30-31 0.08575759852153197 1103 32-33 0.06397552186118816 1103 34-35 0.0675893353330892 1103 36-37 -0.0876711145265503 1103 38-39 0.07871331821129246 1103 40-41 0.12761421316081112 1103 42-43 0.10288981960180621 1103 44-45 0.09847656670147131 1103 46-47 0.06585226787029086 1103 48-49 0.06285347755356696 1103 50-51 -0.025955148856205312 1103 52-53 -0.010450373161525306 1103 54-55 0.08978483875336707 1103 56-57 -0.004272846957142917 1103 58-59 -0.032982375073565606 1103 60-61 0.03904879207608758 1103 62-63 -0.05242639108363534 1103 64-65 0.01599864534244233 1103 66-67 0.05416544870752915 1103 68-69 0.09575624077120182 1103 70-71 -0.07779497497266163 1103 72-73 -0.047458074538319295 1103 74-75 -0.06716423431026541 1103 76-77 -0.054820827557211516 1103 78-79 0.0493430101538479 1103 80-81 0.08646842804496657 1103 82-83 0.1751961726554505 1103 84-85 0.13534532431905788 1103 86-87 0.06210473144978934 1103 88-89 0.3650954128161601 1103 90-91 0.4295060311790735 1103 92-93 0.4580108105490801 1103 94-95 0.26596421417291793 1103 96-97 -0.011562544482256243 1103 98-99 0.23885604975423647 1103 100-101 0.542122023721145 1104 1 0.017022493034019703 1104 2 0.04530093589453088 1104 3 0.03332871106667312 1104 4 0.07024757795547742 1104 5 0.052395822613078735 1104 6 0.0978139910934317 1104 7 0.018649019218067053 1104 8 0.06377653681667539 1104 9 0.08265767525731604 1104 10-11 -0.023841839283072375 1104 12-13 0.025525162900308374 1104 14-15 0.07255854095222958 1104 16-17 0.04950720488808713 1104 18-19 0.062405570698963686 1104 20-21 0.00402179233031319 1104 22-23 0.04437218841207624 1104 24-25 0.06177165651710936 1104 26-27 0.05259430177803637 1104 28-29 -0.014279808328261367 1104 30-31 -0.07992278441431466 1104 32-33 -0.015624354972310073 1104 34-35 -0.012115960826349692 1104 36-37 -0.04504078197700068 1104 38-39 0.144385975248575 1104 40-41 8.033857058791227E-4 1104 42-43 -0.025011591214450846 1104 44-45 0.05766758674402439 1104 46-47 0.07882203609333516 1104 48-49 -0.005454830586614889 1104 50-51 -0.20637783388588105 1104 52-53 -0.23669911350415873 1104 54-55 -0.05331072725267205 1104 56-57 -0.15430195903865496 1104 58-59 -0.09358997780562106 1104 60-61 0.0075679948529341345 1104 62-63 0.07963754352615382 1104 64-65 0.14641018940245942 1104 66-67 0.015088245706742498 1104 68-69 0.10942117190992917 1104 70-71 -0.020264015393671286 1104 72-73 -0.13943097790860293 1104 74-75 -0.09375817675791964 1104 76-77 -0.2928371191259096 1104 78-79 -0.12512726193214618 1104 80-81 0.08509069878732589 1104 82-83 -0.005718618521353136 1104 84-85 -0.02216178574700578 1104 86-87 -0.18327568507691794 1104 88-89 0.07857380269411607 1104 90-91 0.36271955710002146 1104 92-93 0.18577718609493132 1104 94-95 0.3344516735839598 1104 96-97 0.3959381833197817 1104 98-99 0.46430023293640943 1104 100-101 1.0627608099712837 1105 1 -0.044745103247521456 1105 2 -0.005543685619414873 1105 3 0.01836191159787859 1105 4 0.0013604597615852754 1105 5 0.06326700455198875 1105 6 0.0870636590881162 1105 7 -0.02969493828525316 1105 8 0.03759990999064655 1105 9 -0.003068752365521732 1105 10-11 -0.01368513277576966 1105 12-13 0.006579611771485361 1105 14-15 0.05226106419259935 1105 16-17 0.09457360595050801 1105 18-19 0.011254176276658256 1105 20-21 0.09253905660654027 1105 22-23 0.004311763445272732 1105 24-25 0.009286264750841156 1105 26-27 0.04821116764789224 1105 28-29 0.05723612792672128 1105 30-31 0.11342316512088502 1105 32-33 0.03120101023352362 1105 34-35 0.07151484926661311 1105 36-37 0.10986028043999596 1105 38-39 0.13752342544778173 1105 40-41 0.17891427548011052 1105 42-43 0.05780713388515579 1105 44-45 0.06320543534959455 1105 46-47 0.1499986629193657 1105 48-49 -0.040448854490996666 1105 50-51 0.032486707946787874 1105 52-53 0.25703594625679926 1105 54-55 0.2507058730129259 1105 56-57 0.1031083995270734 1105 58-59 0.08977589204829428 1105 60-61 0.010243957668336634 1105 62-63 0.012779641799724573 1105 64-65 -0.0021575396812067993 1105 66-67 0.056928219146321624 1105 68-69 -0.028214286257401966 1105 70-71 0.03369747199013062 1105 72-73 0.02717592780189193 1105 74-75 0.14324248725270294 1105 76-77 -0.03894535285764533 1105 78-79 0.14777712053797742 1105 80-81 0.09688817554695461 1105 82-83 0.27382985425421325 1105 84-85 0.11156729791833087 1105 86-87 0.10278341186863571 1105 88-89 0.29446822500487 1105 90-91 0.407188348600684 1105 92-93 0.39449630960090687 1105 94-95 0.28969802152552404 1105 96-97 0.1996805471976053 1105 98-99 0.4194184268275123 1105 100-101 0.8674088843404739 1106 1 -0.004675522969485257 1106 2 -0.02723287973628885 1106 3 -0.03335110916790285 1106 4 0.006425151122193995 1106 5 -0.026155531108280172 1106 6 -0.060921622191671077 1106 7 0.012179327230782633 1106 8 -0.0057621017890454596 1106 9 -0.0146931961665544 1106 10-11 0.06373275240825649 1106 12-13 0.05347309669402023 1106 14-15 0.07215911094017002 1106 16-17 0.061204015147872326 1106 18-19 -0.03752833262230837 1106 20-21 -0.007510214684920413 1106 22-23 0.0309543982114846 1106 24-25 0.04505495148587357 1106 26-27 0.02040143758862456 1106 28-29 0.028377376808215615 1106 30-31 0.06749796533905084 1106 32-33 0.1323000668187646 1106 34-35 0.017006476968234097 1106 36-37 -0.037065280497223796 1106 38-39 0.12325887889869591 1106 40-41 0.1562421120020474 1106 42-43 0.25241770835805966 1106 44-45 0.1182666221490436 1106 46-47 0.16002460103907623 1106 48-49 0.027278864469835185 1106 50-51 0.06904856094038081 1106 52-53 0.1718515277322794 1106 54-55 0.11046812838691977 1106 56-57 0.01730058398446488 1106 58-59 0.23213122136132114 1106 60-61 0.2694462147367389 1106 62-63 0.18826639368509035 1106 64-65 0.054337241907120415 1106 66-67 0.3190836967645154 1106 68-69 0.16050420380732788 1106 70-71 0.3127079236614634 1106 72-73 0.2828566467762599 1106 74-75 0.2876455829702387 1106 76-77 0.10300586015722502 1106 78-79 0.38205261310896077 1106 80-81 0.3802072722828882 1106 82-83 0.40892020642658267 1106 84-85 0.37385575234353396 1106 86-87 0.18680570262449692 1106 88-89 0.24922035536319243 1106 90-91 0.48926893778619984 1106 92-93 0.5221150683704998 1106 94-95 0.42346521079759114 1106 96-97 0.34634517663338826 1106 98-99 0.47313658062905617 1106 100-101 1.1295453106837705 1107 1 -0.01488910679348976 1107 2 -0.04054554965958346 1107 3 -0.08297573050297302 1107 4 -0.08188955011696919 1107 5 -0.11188705793887976 1107 6 -0.07766466349906409 1107 7 -0.12172139906567736 1107 8 -0.05485347611993774 1107 9 -0.013373372608143086 1107 10-11 -0.046252792280050414 1107 12-13 0.01577182094256102 1107 14-15 -0.08932459833194173 1107 16-17 -0.03301543418047714 1107 18-19 -0.12232534780039117 1107 20-21 -0.002918483650709902 1107 22-23 0.012437518295648431 1107 24-25 -0.06980208216335626 1107 26-27 -0.07560168787267685 1107 28-29 0.00866521107666074 1107 30-31 -0.15072550110254213 1107 32-33 -0.16872116394169723 1107 34-35 -0.11354890606136792 1107 36-37 -0.07054466296018091 1107 38-39 -0.09962113988725463 1107 40-41 -0.14782641162055654 1107 42-43 -0.16749218629853857 1107 44-45 -0.24034448742587955 1107 46-47 -0.25318184488985196 1107 48-49 -0.20626832778368254 1107 50-51 -0.21882069718903097 1107 52-53 -0.16754322734633575 1107 54-55 -0.27549119297066227 1107 56-57 -0.11243477490756959 1107 58-59 -0.10142158625754405 1107 60-61 -0.2530637429650895 1107 62-63 -0.20347457376578149 1107 64-65 -0.3143140451814119 1107 66-67 -0.1745402579586255 1107 68-69 -0.023754119163811538 1107 70-71 -0.19860209215089242 1107 72-73 -0.20466814910308173 1107 74-75 -0.28155916856472984 1107 76-77 -0.34565018510262036 1107 78-79 -0.14698754653757717 1107 80-81 -0.08418679151021635 1107 82-83 -0.03461038221259827 1107 84-85 -0.18163612813596686 1107 86-87 -0.23372523229554432 1107 88-89 0.0587163210209809 1107 90-91 0.19816802626776564 1107 92-93 0.3700404461208038 1107 94-95 0.3474040237348852 1107 96-97 0.38446297849011657 1107 98-99 0.4342515050364568 1107 100-101 0.9745546866422607 1108 1 0.009013808999284834 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quality scores pass #Quality Count 2 1.0 3 8.0 4 24.0 5 81.0 6 217.0 7 447.0 8 929.0 9 1858.0 10 3068.0 11 4594.0 12 6690.0 13 9038.0 14 11227.0 15 14983.0 16 19619.0 17 27673.0 18 27578.0 19 19167.0 20 17324.0 21 18489.0 22 19858.0 23 22069.0 24 24810.0 25 30129.0 26 36138.0 27 43293.0 28 52615.0 29 64647.0 30 78077.0 31 95297.0 32 117786.0 33 150428.0 34 203027.0 35 291420.0 36 452712.0 37 701612.0 38 516442.0 39 35488.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.66940250796898 19.792539188222577 14.901729258586577 22.63632904522186 2 15.744615906501824 21.198334136510645 39.235099457719045 23.821950499268482 3 19.298923998906012 24.974902712943788 30.53077355433695 25.19539973381325 4 13.17787924637921 17.261418664429954 36.68875484431346 32.87194724487738 5 13.55787321745309 37.72109096552193 34.14381317228262 14.57722264474236 6 31.59994523645316 36.2802405876757 17.16343423869532 14.956379937175823 7 27.960830597560715 33.387712124578734 20.813738852908898 17.837718424951657 8 26.208813917123003 35.12446683294521 19.54734786362851 19.11937138630328 9 26.287817066668207 16.911098692055408 20.65005740874158 36.151026832534804 10-11 25.06572106565758 26.69205091727338 26.902319851817797 21.33990816525125 12-13 26.126540344991106 24.17853878160086 27.681001057116006 22.013919816292027 14-15 22.87024300779459 25.4072520785038 26.420404446539248 25.302100467162365 16-17 21.936901089077608 28.66682741297415 27.0589902609541 22.337281236994137 18-19 21.84382577881747 27.292269650831084 29.343610155367518 21.520294414983923 20-21 22.724579438083687 26.251858449697856 29.26792552285881 21.755636589359646 22-23 22.454080220901016 26.222617024216838 29.306481240118593 22.016821514763553 24-25 22.43306294633653 26.350820795911844 29.331025441002055 21.885090816749567 26-27 22.183116090703567 26.38912000943934 29.575970473855378 21.85179342600172 28-29 21.902741479827746 26.79098120052083 29.558752660825437 21.747524658825988 30-31 22.113683736669422 26.377304806270747 29.352700006380527 22.156311450679304 32-33 21.975540445348194 26.642657917324357 29.30450936767662 22.07729226965083 34-35 22.45681621973889 26.481979804684023 29.21480027927475 21.846403696302335 36-37 22.43709227214937 26.285466010187086 29.292114709706592 21.985327007956958 38-39 22.15187123385497 26.64128686619376 29.37950267926101 21.827339220690266 40-41 22.209475060255993 26.57230702932592 29.264115388820684 21.954102521597406 42-43 22.064036857239916 26.681131425436565 28.81370007385748 22.441131643466033 44-45 22.24215122985294 26.613139432710504 28.762169755921136 22.38253958151542 46-47 22.497497878627513 26.916060661393683 28.41273243517209 22.173709024806712 48-49 22.518212209574955 27.00189220052035 28.233577990387822 22.246317599516875 50-51 22.23463065591718 27.10617531193023 28.333247564444097 22.325946467708494 52-53 22.5366102967652 27.301664100026198 27.866597538910813 22.29512806429779 54-55 22.226946165958555 26.94579723444088 28.00518971176355 22.822066887837007 56-57 22.39883572955914 27.387624913309754 27.747627901578237 22.465911455552874 58-59 22.65097951702522 27.030145804947548 27.840538453411725 22.47833622461551 60-61 22.514049066604592 26.98652030275091 28.315572504373876 22.18385812627062 62-63 22.261542106851117 27.062618653015534 28.332231970432815 22.34360726970053 64-65 22.099531302803783 27.14067136112 28.202117569956698 22.557679766119524 66-67 22.21143467171815 27.18865122611217 27.826584496740196 22.77332960542949 68-69 21.945737446965076 27.20232908786744 28.14084184149922 22.711091623668263 70-71 22.25974658072509 27.066722712732172 27.946787018217854 22.72674368832488 72-73 22.26152607536785 26.912868567808207 27.91611879072598 22.909486566097968 74-75 21.950451174033613 27.47345747472717 27.631960749798886 22.94413060144033 76-77 22.060909965706344 27.039389150748434 27.779082393862613 23.12061848968261 78-79 22.032950816492114 27.09895168443731 27.84524989174732 23.022847607323254 80-81 22.003066502119523 27.304116916966215 27.740590080423537 22.952226500490724 82-83 22.0396663784206 27.075748437812113 27.908856528805533 22.97572865496176 84-85 21.920953244820307 27.050338536832175 27.71534049427628 23.31336772407124 86-87 21.8604445066649 26.97872830944517 27.913474746449847 23.247352437440078 88-89 21.830129365256226 26.93716195066782 27.680274728884275 23.552433955191677 90-91 21.79330776993975 26.864581895673943 27.83926268301102 23.50284765137528 92-93 21.891359126707393 26.968097027666815 27.688247287553185 23.452296558072604 94-95 21.699093547873055 26.678953195443338 27.77316605442432 23.848787202259285 96-97 21.46246556622883 26.69399405133207 27.932271178843354 23.91126920359575 98-99 21.44533119922228 26.73594511846144 27.61610561284673 24.202618069469548 100-101 21.48998815912368 26.127183772773165 28.311124876837894 24.07170319126526 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2000.0 1 1368.0 2 862.5 3 1003.5 4 897.0 5 896.5 6 1636.5 7 3366.0 8 5202.0 9 6056.5 10 5321.0 11 4328.5 12 4308.5 13 4485.5 14 4693.5 15 4921.0 16 5128.0 17 5351.0 18 5547.5 19 5866.5 20 6510.0 21 7529.0 22 8803.0 23 10412.5 24 12333.0 25 14852.5 26 18321.0 27 22145.0 28 25113.0 29 28828.0 30 34676.5 31 40617.5 32 45613.5 33 51100.0 34 57125.5 35 62331.0 36 69025.5 37 76005.0 38 80372.0 39 82595.0 40 84422.0 41 88631.0 42 92549.5 43 97416.0 44 103425.5 45 106933.0 46 108219.5 47 115647.5 48 136360.5 49 139584.0 50 124311.0 51 116637.5 52 111210.5 53 108376.0 54 105989.5 55 99259.0 56 90104.5 57 80773.0 58 72797.0 59 64509.5 60 54851.0 61 44439.5 62 35805.5 63 28994.0 64 22608.0 65 17022.5 66 12253.0 67 9379.0 68 6716.5 69 4114.5 70 2743.0 71 1826.0 72 1303.5 73 1016.0 74 788.5 75 563.5 76 355.5 77 170.5 78 81.0 79 53.0 80 28.0 81 17.0 82 11.0 83 5.0 84 4.5 85 3.5 86 1.5 87 0.5 88 1.0 89 1.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3990236185430395 2 0.0 3 0.0 4 0.0 5 3.5269263189822704E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0010580778956946811 16-17 0.002468848423287589 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.002260439140802273 36-37 0.00666909703953011 38-39 0.011943455034735415 40-41 0.0027574151221134114 42-43 5.931648809197454E-4 44-45 4.649130147749356E-4 46-47 3.0459818209392333E-4 48-49 2.4047224902151842E-4 50-51 5.290389478473406E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.4428334941291105E-4 60-61 0.001971872441976451 62-63 0.0 64-65 1.6031483268101227E-4 66-67 0.0013306131112524018 68-69 0.0014588649773972118 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.12361876748032856 78-79 8.015741634050614E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14393065678101283 88-89 0.4343570076659347 90-91 0.33704590422856023 92-93 0.0 94-95 0.0 96-97 4.3285004823873314E-4 98-99 0.0 100-101 1.9237779921721474E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3118863.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.44120292500681 #Duplication Level Percentage of deduplicated Percentage of total 1 70.54259983529317 32.05540593973109 2 14.835514256958932 13.482872276946049 3 5.64486720320608 7.695286681968094 4 2.7486510939712634 4.996080485247605 5 1.5554959591622104 3.5341803764659057 6 0.9797791547936471 2.671340603680186 7 0.6722045739063657 2.138204911499783 8 0.4879475606374975 1.773833929575247 9 0.36808114357005756 1.5053444944051995 >10 1.9428559083670818 15.950802809665312 >50 0.14496176627707186 4.586265085824104 >100 0.07166980045243707 5.720737329574734 >500 0.003392680044963622 1.0270443634267497 >1k 0.0016963400222051186 1.4553087264350493 >5k 1.4136166851709322E-4 0.5342544935119642 >10k+ 1.4136166851709322E-4 0.8730374920429367 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14199 0.45526206184753865 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12983 0.41627349453951645 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8445 0.27077175239822976 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8189 0.2625636329649619 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4240 0.1359469781134984 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 3.206296653620246E-5 0.0 0.0 0.0 0.0 8 3.206296653620246E-5 0.0 0.0 0.0 0.0 9 3.206296653620246E-5 0.0 0.0 3.206296653620246E-5 0.0 10-11 3.206296653620246E-5 0.0 0.0 4.8094449804303685E-5 0.0 12-13 3.206296653620246E-5 0.0 0.0 8.015741634050615E-5 0.0 14-15 6.412593307240492E-5 0.0 0.0 9.618889960860737E-5 0.0 16-17 9.618889960860737E-5 0.0 0.0 2.0840928248531596E-4 0.0 18-19 1.4428334941291105E-4 0.0 0.0 2.885666988258221E-4 0.0 20-21 1.6031483268101227E-4 0.0 0.0 3.0459818209392333E-4 0.0 22-23 1.6031483268101227E-4 0.0 0.0 5.931648809197454E-4 0.0 24-25 2.5650373228961967E-4 0.0 0.0 8.336371299412638E-4 0.0 26-27 2.5650373228961967E-4 0.0 0.0 0.0010741093789627823 0.0 28-29 3.847555984344295E-4 0.0 0.0 0.003078044787475436 1.603148326810123E-5 30-31 4.8094449804303685E-4 0.0 0.0 0.008849378763991878 3.206296653620246E-5 32-33 4.969759813111381E-4 0.0 0.0 0.020311889300684255 3.206296653620246E-5 34-35 5.450704311154418E-4 0.0 0.0 0.03887634692514548 4.8094449804303685E-5 36-37 5.450704311154418E-4 0.0 0.0 0.06888728360303098 6.412593307240492E-5 38-39 5.771333976516442E-4 0.0 0.0 0.11383956268678681 6.412593307240492E-5 40-41 6.091963641878467E-4 0.0 0.0 0.1629439959369809 6.412593307240492E-5 42-43 7.214167470645553E-4 0.0 0.0 0.2157677333053744 8.015741634050615E-5 44-45 8.657000964774663E-4 0.0 0.0 0.2817693499201472 9.618889960860737E-5 46-47 9.458575128179725E-4 0.0 0.0 0.3612855069299293 1.122203828767086E-4 48-49 9.618889960860737E-4 0.0 0.0 0.44865709074108095 1.2825186614480983E-4 50-51 9.618889960860737E-4 0.0 0.0 0.5414473158968509 1.2825186614480983E-4 52-53 9.618889960860737E-4 0.0 0.0 0.6347826114837363 1.4428334941291105E-4 54-55 9.618889960860737E-4 0.0 0.0 0.7428989346438108 1.6031483268101227E-4 56-57 9.618889960860737E-4 0.0 0.0 0.8758800883527106 1.6031483268101227E-4 58-59 9.618889960860737E-4 0.0 0.0 1.025277480928146 1.6031483268101227E-4 60-61 9.618889960860737E-4 0.0 0.0 1.19317520519497 1.6031483268101227E-4 62-63 9.618889960860737E-4 0.0 0.0 1.3646479502305808 1.6031483268101227E-4 64-65 9.618889960860737E-4 0.0 0.0 1.542517257090164 1.6031483268101227E-4 66-67 0.0010260149291584787 0.0 0.0 1.751134948857965 1.6031483268101227E-4 68-69 0.0010580778956946811 0.0 0.0 1.991511008979875 1.6031483268101227E-4 70-71 0.0010901408622308836 0.0 0.0 2.260407077835737 1.7634631594911352E-4 72-73 0.0010901408622308836 0.0 0.0 2.5373028568423814 2.885666988258221E-4 74-75 0.0010901408622308836 0.0 0.0 2.8186072937477533 2.885666988258221E-4 76-77 0.0010901408622308836 0.0 0.0 3.126235426179348 2.885666988258221E-4 78-79 0.0010901408622308836 0.0 0.0 3.477148563434816 2.885666988258221E-4 80-81 0.0012183927283756933 0.0 0.0 3.867418992113472 2.885666988258221E-4 82-83 0.0012504556949118957 0.0 0.0 4.2669556181210915 3.5269263189822704E-4 84-85 0.001266487178179997 0.0 0.0 4.6797021863416255 3.5269263189822704E-4 86-87 0.0012985501447161994 0.0 0.0 5.113898879174879 3.6872411516632826E-4 88-89 0.0013145816279843006 0.0 0.0 5.587356674531712 3.847555984344295E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12960 0.0 24.372585 1 TACATGG 13045 0.0 23.2801 2 ACATGGG 13115 0.0 22.106596 3 GAGTACT 11915 0.0 21.924322 12-13 GGTATCA 19955 0.0 21.606773 1 GTATCAA 25215 0.0 21.105093 1 GTACTTT 13115 0.0 19.84592 14-15 AGTACTT 12485 0.0 19.76413 12-13 CATGGGG 6380 0.0 19.114433 4 AGAGTAC 21080 0.0 18.582724 10-11 CATGGGA 7550 0.0 18.35206 4 ATCAACG 29510 0.0 17.752062 3 GTATAGG 780 0.0 17.713177 1 TCAACGC 29630 0.0 17.680168 4 TATCAAC 29860 0.0 17.639332 2 CAACGCA 29700 0.0 17.62252 5 AACGCAG 30000 0.0 17.47793 6 AGTACAT 10110 0.0 17.131313 2 ACTTTTT 15615 0.0 16.744108 16-17 TACTTTT 15040 0.0 16.359266 14-15 >>END_MODULE