##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938975_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 490562 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.730631398273818 33.0 31.0 34.0 30.0 34.0 2 31.696140345155147 33.0 31.0 34.0 30.0 34.0 3 31.76862048018395 33.0 31.0 34.0 30.0 34.0 4 35.21321260105756 37.0 35.0 37.0 33.0 37.0 5 35.31282488248173 37.0 35.0 37.0 33.0 37.0 6 35.48994010950705 37.0 35.0 37.0 33.0 37.0 7 35.46604098972199 37.0 35.0 37.0 33.0 37.0 8 35.50567716211203 37.0 35.0 37.0 33.0 37.0 9 36.97268235207782 39.0 37.0 39.0 33.0 39.0 10-11 36.88134425414117 39.0 37.0 39.0 32.5 39.0 12-13 36.848988507059246 39.0 37.0 39.0 33.0 39.0 14-15 37.7771433580261 40.0 37.0 41.0 32.0 41.0 16-17 37.77337319237935 40.0 37.0 41.0 32.5 41.0 18-19 37.78592614185363 40.0 37.0 41.0 33.0 41.0 20-21 37.89162022333568 40.0 37.0 41.0 33.0 41.0 22-23 37.85853266253807 40.0 37.5 41.0 33.0 41.0 24-25 37.66326070914584 40.0 37.0 41.0 32.5 41.0 26-27 37.41284587880838 39.5 36.5 41.0 31.5 41.0 28-29 37.37146069202262 39.5 36.5 41.0 32.0 41.0 30-31 37.37538272430396 40.0 36.0 41.0 32.0 41.0 32-33 37.1579851272622 40.0 36.0 41.0 31.5 41.0 34-35 36.75022423261484 39.0 35.0 41.0 30.5 41.0 36-37 36.36233136688125 39.0 35.0 41.0 30.0 41.0 38-39 36.05650559969993 38.5 35.0 40.0 30.0 41.0 40-41 36.147776427852136 39.0 35.0 40.0 30.0 41.0 42-43 36.02097899959638 39.0 35.0 40.0 30.0 41.0 44-45 36.00602981886082 39.0 35.0 41.0 30.0 41.0 46-47 35.74739992090704 39.0 35.0 41.0 28.5 41.0 48-49 35.43196068998414 39.0 35.0 40.5 26.5 41.0 50-51 34.24910612725813 38.0 34.0 39.5 25.0 40.5 52-53 34.50630399419441 38.0 33.5 39.5 25.0 40.5 54-55 35.17402693237552 39.0 35.0 40.0 26.5 41.0 56-57 35.06542598081384 38.5 35.0 40.0 25.5 41.0 58-59 34.91357361556745 39.0 35.0 40.0 26.0 41.0 60-61 34.3924763842287 38.0 34.0 40.0 23.0 41.0 62-63 34.07849976149804 38.0 34.0 40.0 22.5 41.0 64-65 33.64289427228363 37.0 33.0 39.5 22.0 41.0 66-67 32.989649626346925 36.0 32.0 39.0 21.5 40.0 68-69 32.48740628095939 36.0 32.0 39.0 20.5 40.0 70-71 31.666671083369685 35.0 31.5 37.5 16.0 39.0 72-73 31.095389573591106 35.0 31.0 37.0 13.0 39.0 74-75 30.695572628943946 35.0 31.0 36.5 9.0 37.5 76-77 30.226992714478495 34.0 30.0 36.0 4.5 37.0 78-79 29.745677406729428 34.0 29.5 35.5 2.0 37.0 80-81 29.186686901961423 34.0 29.0 35.0 2.0 36.0 82-83 28.64853576102511 34.0 28.5 35.0 2.0 36.0 84-85 28.158347976402574 34.0 28.0 35.0 2.0 35.0 86-87 27.882440955475555 34.0 27.5 35.0 2.0 35.0 88-89 27.216749360937047 33.5 25.5 35.0 2.0 35.0 90-91 26.565019304389658 33.0 25.0 35.0 2.0 35.0 92-93 25.9841192346737 33.0 23.5 35.0 2.0 35.0 94-95 25.312744362588216 32.0 20.0 35.0 2.0 35.0 96-97 24.519496006620976 32.0 12.0 34.5 2.0 35.0 98-99 23.70674043240202 32.0 2.0 34.0 2.0 35.0 100-101 20.551269768143477 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.255329075789799 1101 2 0.5760832383499555 1101 3 0.3658919885444547 1101 4 0.3325450753502608 1101 5 -0.11094320170820993 1101 6 0.28368985991443196 1101 7 0.3023942682840044 1101 8 0.5323341195413036 1101 9 0.5817812073878912 1101 10-11 0.48254345022652956 1101 12-13 0.4664975783048675 1101 14-15 0.7137317235310405 1101 16-17 0.6020934471422805 1101 18-19 0.4641683649862429 1101 20-21 0.3977289458670299 1101 22-23 0.6445433639296283 1101 24-25 0.7136694305094053 1101 26-27 0.5858424461838467 1101 28-29 0.8621881780833576 1101 30-31 0.8300758876265277 1101 32-33 0.7617220019510995 1101 34-35 0.8361372244808081 1101 36-37 0.8828200961242558 1101 38-39 1.1194875396187243 1101 40-41 1.0531392391385097 1101 42-43 0.9730441172417201 1101 44-45 1.0936587050324533 1101 46-47 1.0676200974445962 1101 48-49 0.8307521540766842 1101 50-51 0.7474408489199078 1101 52-53 1.1590733673034421 1101 54-55 1.5342013483095585 1101 56-57 1.622341488276433 1101 58-59 1.6224984480310454 1101 60-61 1.7391487000983048 1101 62-63 1.506047173627529 1101 64-65 1.4073004737771484 1101 66-67 1.332354928814297 1101 68-69 1.416070400368362 1101 70-71 1.1315148947287668 1101 72-73 0.7337184353941133 1101 74-75 0.7782273806319182 1101 76-77 0.8575559027836306 1101 78-79 1.2135785447027132 1101 80-81 1.3160088797306777 1101 82-83 1.07052364432084 1101 84-85 1.240010589793254 1101 86-87 0.9111733218350544 1101 88-89 0.3598195593479474 1101 90-91 0.5238765774768908 1101 92-93 0.08057124909496949 1101 94-95 0.19275741658656997 1101 96-97 -0.11574108145206097 1101 98-99 -0.2310231530895095 1101 100-101 0.1128325206689702 1102 1 0.30327871516079696 1102 2 0.3565557515390374 1102 3 0.6743151605504707 1102 4 0.40375337364013575 1102 5 0.38202516998177316 1102 6 0.4830832895854016 1102 7 0.5405419350271288 1102 8 0.4324895850458006 1102 9 0.21185486235641093 1102 10-11 0.5248581022131802 1102 12-13 0.4268844580357296 1102 14-15 0.42723173627411626 1102 16-17 0.7010485150628654 1102 18-19 0.5720639737228908 1102 20-21 0.631434376965224 1102 22-23 0.6828413989475877 1102 24-25 0.47892416456144105 1102 26-27 0.6371511598979467 1102 28-29 0.39352849462130735 1102 30-31 0.4610899814665217 1102 32-33 0.6094753979803613 1102 34-35 0.5812680958522165 1102 36-37 0.8183834674319499 1102 38-39 0.9646770291311739 1102 40-41 0.8370345420413798 1102 42-43 0.8559582034358826 1102 44-45 1.0697348576435104 1102 46-47 1.0950737763700928 1102 48-49 1.2628596036778177 1102 50-51 0.9125018882450391 1102 52-53 1.1116079647497301 1102 54-55 1.0343848485771687 1102 56-57 1.341575884382145 1102 58-59 1.1349152994722829 1102 60-61 1.220059575037773 1102 62-63 1.193821468298374 1102 64-65 0.6821940691080854 1102 66-67 0.798269754106748 1102 68-69 0.940256755084576 1102 70-71 1.1673152872154589 1102 72-73 1.063600944250556 1102 74-75 1.076507065623126 1102 76-77 1.26601985262689 1102 78-79 1.278598806190594 1102 80-81 1.2171022354918186 1102 82-83 1.2772290499328918 1102 84-85 1.0218797439079914 1102 86-87 0.7454088648174455 1102 88-89 0.5867074457816734 1102 90-91 0.5603192209001904 1102 92-93 0.8629985498570036 1102 94-95 0.9452741737290609 1102 96-97 1.066813650051369 1102 98-99 1.084645730872058 1102 100-101 1.5792264855490608 1103 1 0.06419825539068569 1103 2 -0.05333930093472716 1103 3 0.13063699963093 1103 4 -0.013213143101488356 1103 5 0.004413975578977158 1103 6 -0.025362487526038535 1103 7 0.21742849174876966 1103 8 0.14033566200731684 1103 9 0.18806675640938408 1103 10-11 0.09597254499178831 1103 12-13 -0.09429245350849413 1103 14-15 0.1393631705569689 1103 16-17 0.07778514674702564 1103 18-19 0.18700650245852302 1103 20-21 0.3751125378847675 1103 22-23 0.06934814557428126 1103 24-25 0.17868678325209686 1103 26-27 0.22458244425577334 1103 28-29 0.38865593090316963 1103 30-31 0.23389108091679844 1103 32-33 0.3789156778404319 1103 34-35 0.3971851373314763 1103 36-37 0.3990931126093571 1103 38-39 0.5938874239337792 1103 40-41 0.5173443871188468 1103 42-43 0.4582195727512186 1103 44-45 0.6943600450498124 1103 46-47 0.3810058103531091 1103 48-49 0.626852607176076 1103 50-51 0.5166373205288934 1103 52-53 0.425625955754235 1103 54-55 0.5802993913058074 1103 56-57 0.3056563441522613 1103 58-59 0.28491529947228145 1103 60-61 0.32280820072492844 1103 62-63 0.4077270155247632 1103 64-65 0.49884824202163003 1103 66-67 0.6965456161757118 1103 68-69 0.736221272825702 1103 70-71 0.8590069413883725 1103 72-73 0.4530812041206147 1103 74-75 0.4502701840639034 1103 76-77 0.43858357076782184 1103 78-79 0.4621445333270273 1103 80-81 0.6072071830180583 1103 82-83 0.583600864025847 1103 84-85 0.7476168753422776 1103 86-87 0.6426227578709174 1103 88-89 0.6217399295397925 1103 90-91 0.6632552528841984 1103 92-93 0.3662094444097299 1103 94-95 0.1828928643837351 1103 96-97 0.35067172101588895 1103 98-99 0.37667471637930205 1103 100-101 0.7998661657089805 1104 1 0.29883029002271755 1104 2 0.3377768293118386 1104 3 0.3886456576395929 1104 4 0.407933101957795 1104 5 0.4402129592040467 1104 6 0.2972048002586405 1104 7 0.3182848817355932 1104 8 0.41244181672216484 1104 9 0.46004115881856933 1104 10-11 0.4975629835387494 1104 12-13 0.4987011470938043 1104 14-15 0.6915323781609644 1104 16-17 0.5988466938955312 1104 18-19 0.5885216539736788 1104 20-21 0.793727256499487 1104 22-23 0.7666001689132571 1104 24-25 0.6446429285560669 1104 26-27 0.8342850604801271 1104 28-29 1.003223971558171 1104 30-31 1.026517657239026 1104 32-33 0.6902934305225301 1104 34-35 0.8261330010619545 1104 36-37 1.0872071724625485 1104 38-39 0.9286417999924979 1104 40-41 0.939714046445026 1104 42-43 0.9700349313057117 1104 44-45 0.9725785656596315 1104 46-47 1.203798959233211 1104 48-49 1.2703308680456402 1104 50-51 1.0781609418351223 1104 52-53 1.3088463585398529 1104 54-55 1.4642773698489933 1104 56-57 1.5148554783514 1104 58-59 1.548984563541545 1104 60-61 1.596608313655473 1104 62-63 1.545008212588563 1104 64-65 1.6138256559120876 1104 66-67 1.5331257027557967 1104 68-69 1.4914584269759033 1104 70-71 1.404268563823905 1104 72-73 1.3954489812710058 1104 74-75 1.0848739860590086 1104 76-77 1.2352615304892574 1104 78-79 1.111424601085357 1104 80-81 0.9747302396715476 1104 82-83 1.006591641364448 1104 84-85 0.971849888705723 1104 86-87 1.0622397350965898 1104 88-89 1.284378740004687 1104 90-91 1.2007058457695727 1104 92-93 1.130433987982098 1104 94-95 1.1488866532036077 1104 96-97 1.4785467445430847 1104 98-99 0.9993643455906742 1104 100-101 1.1701654316604184 1105 1 0.13758881333059847 1105 2 0.23010910361836423 1105 3 0.1297786305322184 1105 4 0.19002440121647624 1105 5 0.13749850627500138 1105 6 0.21514880189362628 1105 7 0.37362367741773284 1105 8 0.27368143547251833 1105 9 0.2566429833173913 1105 10-11 0.1949089137172919 1105 12-13 0.37220782639447236 1105 14-15 0.37929132879545335 1105 16-17 0.39364631487541146 1105 18-19 0.5485944852911402 1105 20-21 0.6931382889577336 1105 22-23 0.3234067385953665 1105 24-25 0.5677752325896606 1105 26-27 0.3437837877900165 1105 28-29 0.30988190589850717 1105 30-31 0.2705443744417124 1105 32-33 0.590438349980758 1105 34-35 0.6375751354654611 1105 36-37 0.6409945867649824 1105 38-39 0.5262255467178747 1105 40-41 0.45919921077997117 1105 42-43 0.42791074647765726 1105 44-45 0.12270955055457478 1105 46-47 0.2155365063784771 1105 48-49 0.22097464486967766 1105 50-51 0.23503161050837207 1105 52-53 0.21456045845996385 1105 54-55 0.2770665120372868 1105 56-57 0.35061342569732545 1105 58-59 0.19585950548086117 1105 60-61 0.46226518896900615 1105 62-63 0.2623873999248332 1105 64-65 0.568287503077805 1105 66-67 0.6868889638151927 1105 68-69 0.591926628302474 1105 70-71 0.2854717669155207 1105 72-73 0.3117115456022326 1105 74-75 0.13280423892488713 1105 76-77 -0.00520258336541346 1105 78-79 0.2580719451575959 1105 80-81 0.22713627426642446 1105 82-83 0.6034833047797186 1105 84-85 0.8040266532859199 1105 86-87 0.6342303339112227 1105 88-89 0.20203476066500414 1105 90-91 0.3873035827964948 1105 92-93 0.4846970353781934 1105 94-95 0.1750975667535819 1105 96-97 -0.15526222188390904 1105 98-99 0.34780739038565045 1105 100-101 0.4183957421224882 1106 1 0.5778824659170034 1106 2 0.5260657333902152 1106 3 0.48116331542040314 1106 4 0.6031484129625824 1106 5 0.3539791929702787 1106 6 0.40557572757693805 1106 7 0.3510440523894971 1106 8 0.36422582219038446 1106 9 0.5299431866153341 1106 10-11 0.46389016512909365 1106 12-13 0.5993345487798365 1106 14-15 0.7304501270787114 1106 16-17 0.598265695946111 1106 18-19 0.7806907129848426 1106 20-21 0.6077441168321371 1106 22-23 0.7345655368786268 1106 24-25 0.47034582215369625 1106 26-27 0.42588679208185454 1106 28-29 0.46387332220751887 1106 30-31 0.22484073766737112 1106 32-33 0.5166502316161683 1106 34-35 0.5857206064390326 1106 36-37 0.5550198860647342 1106 38-39 0.6663588198147821 1106 40-41 0.7389462177824626 1106 42-43 0.7480937146276503 1106 44-45 0.7681014638141193 1106 46-47 0.9920126753416625 1106 48-49 0.9929624469930118 1106 50-51 0.8906991054259237 1106 52-53 0.5969074202851274 1106 54-55 0.52131769794196 1106 56-57 0.695393186257526 1106 58-59 0.842283720524911 1106 60-61 1.488529024523551 1106 62-63 1.258104589895467 1106 64-65 0.9328413770330783 1106 66-67 1.0875982477546557 1106 68-69 1.2586354604687244 1106 70-71 0.8849680397865356 1106 72-73 0.8226693048071141 1106 74-75 0.7642747607229445 1106 76-77 0.596981740104205 1106 78-79 0.7400507118167319 1106 80-81 0.7508227436587909 1106 82-83 0.39025990292744694 1106 84-85 0.12702190966721716 1106 86-87 0.5173023917381947 1106 88-89 0.42183146272057215 1106 90-91 0.6322369462480424 1106 92-93 0.6631316412060677 1106 94-95 0.5553528186171413 1106 96-97 0.45190741895639164 1106 98-99 0.23271590631065564 1106 100-101 0.40320712680737714 1107 1 0.13072802951799645 1107 2 0.3478748763708701 1107 3 0.1423413117459198 1107 4 0.3198240857173644 1107 5 0.1257245728470764 1107 6 -0.02361264376209249 1107 7 -0.024786489056516814 1107 8 0.17036869477225025 1107 9 0.2852009408572016 1107 10-11 0.26120779184474685 1107 12-13 0.23373852586834687 1107 14-15 0.505645198947299 1107 16-17 0.41579514585424704 1107 18-19 0.240394592807597 1107 20-21 0.4940037083159794 1107 22-23 0.2967831960162073 1107 24-25 0.19135306304854538 1107 26-27 0.5227656421845239 1107 28-29 0.8276926240555724 1107 30-31 0.6987000265466534 1107 32-33 0.5956775740996889 1107 34-35 0.5565235892554128 1107 36-37 0.5254121912630509 1107 38-39 0.6141400799466297 1107 40-41 0.18894513705188132 1107 42-43 0.10081175202092396 1107 44-45 -0.085416314203826 1107 46-47 0.22394394087717018 1107 48-49 -0.3164247787716121 1107 50-51 -0.0763342891488179 1107 52-53 -0.12643636011042503 1107 54-55 0.2022103813959788 1107 56-57 0.1578637363493911 1107 58-59 0.20021304074538193 1107 60-61 0.269812932445312 1107 62-63 0.07502234629879467 1107 64-65 0.04066370492673599 1107 66-67 0.0861760063194481 1107 68-69 0.2570921772092376 1107 70-71 0.15838422913053307 1107 72-73 0.3181691426975277 1107 74-75 0.25678745930718705 1107 76-77 0.282793909130465 1107 78-79 0.537729302544065 1107 80-81 0.6266965143277616 1107 82-83 0.5993985606600738 1107 84-85 0.32624378365403217 1107 86-87 0.43656749494796543 1107 88-89 0.33812239539105704 1107 90-91 0.6315906693648685 1107 92-93 0.9431467714341011 1107 94-95 0.7786901146292493 1107 96-97 1.2368247430674906 1107 98-99 1.1779379964435854 1107 100-101 1.4108651836538044 1108 1 0.3325743237667531 1108 2 0.31799105952606865 1108 3 0.4507224697164034 1108 4 0.34208228610697944 1108 5 0.32996209895318174 1108 6 0.3787066317604726 1108 7 0.436194749645459 1108 8 0.5011309054112587 1108 9 0.4692819180593304 1108 10-11 0.5054578664336304 1108 12-13 0.5976993710920269 1108 14-15 0.7812620928906782 1108 16-17 0.6609512560000894 1108 18-19 0.5139295793816032 1108 20-21 0.5366509994232374 1108 22-23 0.6708903232034089 1108 24-25 0.5859238698370106 1108 26-27 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-3.008458713766725 2316 98-99 -3.1176731097076527 2316 100-101 -2.9436120951605886 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2090.0 3 436.0 4 335.0 5 430.0 6 431.0 7 499.0 8 583.0 9 836.0 10 1074.0 11 1466.0 12 2009.0 13 2889.0 14 2660.0 15 2656.0 16 3221.0 17 4402.0 18 3778.0 19 3270.0 20 3213.0 21 3606.0 22 4184.0 23 4702.0 24 5301.0 25 6276.0 26 7193.0 27 8450.0 28 9895.0 29 11756.0 30 13800.0 31 16876.0 32 20618.0 33 25853.0 34 34702.0 35 54001.0 36 67674.0 37 88922.0 38 64016.0 39 6458.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.65090025604738 19.430151419713194 15.082663219979874 15.836285104259549 2 13.136912461388425 27.79502915505429 33.14474698962333 25.92331139393395 3 29.55438160474858 25.63218883332007 32.10619425689118 12.707235305040165 4 11.101724313845526 16.604428119579634 44.52055912661974 27.773288439955106 5 9.379853346040894 43.2621016047037 35.58817707047186 11.769867978783545 6 23.259565962307253 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17.966682648658022 28-29 16.976456894766535 33.70859758417688 30.790676607972973 18.524268913083617 30-31 18.621787777309695 29.141016996869507 28.606211818755824 23.630983407064974 32-33 19.68595252059056 29.96884004385853 30.315220440115255 20.029986995435653 34-35 24.05064844238862 26.011210221300647 28.582690207722415 21.355451128588314 36-37 21.692498507239506 24.855204792401313 34.94257865029871 18.509718050060478 38-39 21.45561817541388 27.970051688688287 32.63759758185408 17.93673255404376 40-41 18.635587901968393 30.519755074545422 31.979131581399017 18.86552544208717 42-43 16.89267793138761 31.867281105990784 26.366922683051712 24.87311827956989 44-45 20.155935077916332 27.163079456633948 27.513743916627064 25.16724154882265 46-47 23.83135170563356 30.19949409608078 27.62593832887851 18.34321586940715 48-49 21.83362593285302 27.73638592818235 29.681472816924202 20.748515322040426 50-51 16.087821775383805 30.04524034123324 35.37514044785582 18.491797435527136 52-53 19.222906398895486 32.82388800806263 27.847526926653803 20.10567866638808 54-55 15.587084960412437 27.060463334777353 31.73866779730102 25.613783907509198 56-57 22.045876688736026 27.08582065864033 27.780360386558513 23.087942266065134 58-59 25.91106629333688 25.880558356666445 27.69025230473129 20.518123045265384 60-61 20.23985566497573 27.516985256418785 33.85753363536167 18.385625443243814 62-63 19.504636987760318 27.880093722862153 32.19961986531435 20.415649424063183 64-65 18.263824801010337 33.11530082238476 29.682364256325112 18.93851012027979 66-67 19.62895696773579 27.43682014625787 27.35231059360534 25.581912292401004 68-69 16.34463088142185 29.191707254103648 30.515878788872246 23.947783075602256 70-71 23.259561590338883 28.41569521748885 26.31912707364573 22.005616118526532 72-73 20.2234311826426 23.967941948047024 33.45106527259485 22.357561596715524 74-75 16.99524899253647 33.46673987465863 28.548863261538965 20.98914787126593 76-77 19.907808993999698 25.848404067382795 28.79611982577723 25.44766711284028 78-79 19.40822852532236 26.49271420921191 32.08123469861063 22.017822566855106 80-81 22.26057625945397 27.13977640199309 28.98971301713951 21.609934321413434 82-83 19.21295822187995 25.748188413217925 33.05782331038042 21.981030054521696 84-85 18.737269207175636 27.322572815782546 30.938655628277743 23.00150234876407 86-87 19.233144321292478 28.830054040990046 29.985324855271124 21.951476782446356 88-89 19.454392948914247 27.324288700422056 27.396593873314373 25.82472447734933 90-91 19.87516119549441 23.5015092712376 30.204476292611176 26.41885324065681 92-93 22.051761649751498 28.36882992366662 30.039518808822386 19.539889617759496 94-95 20.015448271340485 26.701537963512166 30.03838506148064 23.244628703666713 96-97 15.130125346862641 26.688833097226023 33.17886486159809 25.002176694313246 98-99 19.64510158027418 28.709219102602578 27.16571930798598 24.479960009137255 100-101 18.847165382393786 24.364827718992082 29.953053374135113 26.834953524479022 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9184.0 1 5233.0 2 1227.0 3 1040.5 4 768.0 5 745.0 6 1292.0 7 2229.0 8 2803.0 9 2702.5 10 1980.0 11 1247.5 12 1065.5 13 976.0 14 860.0 15 887.5 16 1050.5 17 947.5 18 673.0 19 638.5 20 709.5 21 810.0 22 878.0 23 923.5 24 996.5 25 1227.0 26 1784.5 27 2458.5 28 2652.0 29 2817.5 30 3576.0 31 4396.0 32 5594.5 33 7345.0 34 8878.0 35 9831.5 36 10933.5 37 11995.5 38 11178.5 39 9614.5 40 8554.5 41 7468.0 42 6315.0 43 6080.5 44 6555.5 45 7448.5 46 14546.0 47 39699.0 48 94970.5 49 94982.0 50 40643.5 51 15394.5 52 6085.0 53 3355.0 54 2181.5 55 1345.0 56 796.0 57 516.0 58 356.0 59 266.5 60 233.5 61 204.5 62 179.5 63 148.0 64 117.5 65 90.5 66 79.5 67 70.0 68 53.0 69 44.0 70 35.0 71 33.5 72 34.0 73 26.0 74 21.0 75 18.0 76 14.5 77 9.5 78 5.5 79 7.0 80 6.5 81 5.0 82 3.0 83 2.5 84 2.0 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3241180523562771 2 0.08643148062834055 3 0.09764311137022436 4 0.10437008981535464 5 0.11517402489389722 6 0.05748508853111329 7 0.06013511034283129 8 0.06054280600617251 9 0.06482361047125543 10-11 0.06105242558534905 12-13 0.0590139472686429 14-15 0.05697546895193676 16-17 0.05952356684781944 18-19 0.06818709969382056 20-21 0.06941018668384424 22-23 0.08337376315328135 24-25 0.09305653515763554 26-27 0.0870430241233524 28-29 0.08531031755415218 30-31 0.07817564344568066 32-33 0.07124481716887977 34-35 0.3147410520994288 36-37 0.483628980638533 38-39 0.45947301258556517 40-41 0.44204402297772755 42-43 0.47129618682246077 44-45 0.4782270130992617 46-47 0.47435390429751995 48-49 0.49106942649451035 50-51 0.4875020894402746 52-53 0.48597323070274495 54-55 0.47710585002507333 56-57 0.45926916475389457 58-59 0.4412286316510451 60-61 0.4029052392969696 62-63 0.47170388248580203 64-65 0.4909675025786751 66-67 0.4997329593405115 68-69 0.5139003836416192 70-71 0.5101291987557128 72-73 0.498917568013829 74-75 0.5004464267513586 76-77 0.4762904586983909 78-79 0.45835184951137675 80-81 0.46375381705064805 82-83 0.4721115781491432 84-85 0.4742519803816847 86-87 0.4732327412233316 88-89 0.47975587183679125 90-91 0.4917828939053575 92-93 0.4973887092762994 94-95 0.5058483942906299 96-97 0.4966752418654523 98-99 0.4995291115088409 100-101 0.4786347087626029 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 490562.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 14.664405314720666 #Duplication Level Percentage of deduplicated Percentage of total 1 72.82104034029302 10.678772509896813 2 9.661097055798049 2.833484860221542 3 4.307876226750813 1.8951732910417032 4 2.524396007673274 1.480750649255344 5 1.6389112847173957 1.2016829676982725 6 1.1648919903250021 1.0249468976398497 7 0.8868748088631877 0.9103844162409644 8 0.7033834690983903 0.8251760226026476 9 0.6116377992159915 0.8072374134156336 >10 4.7568739748116435 14.04144634113527 >50 0.5490839333870833 5.485137454592896 >100 0.2794072673691234 7.157505065618618 >500 0.020851288609636077 1.9824201629967262 >1k 0.04865300675581751 15.947627415087185 >5k 0.020851288609636077 21.388529890207558 >10k+ 0.004170257721927215 12.339724642348978 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 29186 5.949502815138556 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 18557 3.7828042123115933 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 12791 2.6074176148988304 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 9473 1.9310505094157313 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 8631 1.7594106351490737 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 7804 1.5908284783574755 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 7680 1.5655513472303195 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 7594 1.5480204337066465 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 7532 1.5353818681430687 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 7207 1.4691313228501188 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 7192 1.4660736053750596 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 7095 1.44630036570301 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 6528 1.3307186451457715 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6514 1.327864775502383 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5873 1.197198315401519 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 5414 1.103632160664707 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 5247 1.0695895727757143 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 5140 1.0477778547869587 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 4874 0.9935543315625751 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 4426 0.9022305029741398 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 4301 0.876749524015313 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 4205 0.8571801321749342 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 3408 0.6947134103334542 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3301 0.6729016923446985 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2963 0.6040011252400309 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2963 0.6040011252400309 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2937 0.5987010816165949 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2931 0.5974779946265713 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2921 0.595439516309865 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2748 0.5601738414308487 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2574 0.5247043187201618 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2528 0.5153273184633135 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2439 0.4971848614446288 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 2168 0.4419420990618923 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2128 0.43378818579506767 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1904 0.38812627150085005 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1867 0.38058390172903733 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1704 0.3473567051667271 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1696 0.3457259225133622 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1560 0.31800261740615865 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1442 0.2939485732690261 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1409 0.28722159482389586 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1402 0.28579466000220155 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1318 0.26867144214186994 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1290 0.26296370285509274 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1249 0.25460594175659756 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1210 0.2466558763214436 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1096 0.2234172235109935 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 1075 0.21913641904591064 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1070 0.21811717988755755 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1060 0.21607870157085138 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1041 0.2122055927691097 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1025 0.20894402746237986 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 825 0.16817446112825696 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 809 0.16491289582152713 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 771 0.1571666782180438 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 714 0.14554735181281875 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 691 0.14085885168439463 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 665 0.13555880806095866 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 661 0.1347434167342762 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 633 0.129035677447499 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 628 0.12801643828914594 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 619 0.12618180780411037 No Hit TACCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 570 0.11619326405225028 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 569 0.11598941622057966 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 567 0.11558172055723843 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 502 0.1023316114986485 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 501 0.10212776366697787 No Hit GCTTTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 495 0.1009046766769542 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.038478316706145E-4 0.0 16-17 0.0 0.0 0.0 3.057717475059218E-4 0.0 18-19 0.0 0.0 0.0 6.115434950118436E-4 0.0 20-21 0.0 0.0 0.0 0.0017327065692002233 0.0 22-23 0.0 0.0 0.0 0.003975032717576983 0.0 24-25 0.0 0.0 0.0 0.008357761098495196 0.0 26-27 0.0 0.0 0.0 0.013963576469437095 0.0 28-29 0.0 0.0 0.0 0.04240034898748782 0.0 30-31 0.0 0.0 0.0 0.09988543751860111 0.0 32-33 0.0 0.0 0.0 0.2117978971057685 0.0 34-35 0.0 0.0 0.0 0.38058390172903733 0.0 36-37 0.0 0.0 0.0 0.5963568315523828 0.0 38-39 0.0 0.0 0.0 0.8721629478027242 0.0 40-41 0.0 0.0 0.0 1.1628499557650205 0.0 42-43 0.0 0.0 0.0 1.4754506056319079 0.0 44-45 0.0 0.0 0.0 1.8336112458771776 0.0 46-47 0.0 0.0 0.0 2.2741264101173755 0.0 48-49 0.0 0.0 0.0 2.667756573073332 0.0 50-51 0.0 0.0 0.0 3.064240605672677 0.0 52-53 0.0 0.0 0.0 3.4420725616741614 0.0 54-55 0.0 0.0 0.0 3.8555778882180034 0.0 56-57 0.0 0.0 0.0 4.454992437245445 0.0 58-59 0.0 0.0 0.0 4.98744297356909 0.0 60-61 0.0 0.0 0.0 5.671148600992332 0.0 62-63 0.0 0.0 0.0 6.298286455126977 0.0 64-65 0.0 0.0 0.0 6.835527415494881 0.0 66-67 0.0 0.0 0.0 7.505779086027863 0.0 68-69 0.0 0.0 0.0 8.312303032032649 0.0 70-71 0.0 0.0 0.0 9.11220192350814 0.0 72-73 0.0 0.0 0.0 9.860119617907625 0.0 74-75 0.0 0.0 0.0 10.781307969227132 0.0 76-77 0.0 0.0 0.0 11.503031217256941 0.0 78-79 0.0 0.0 0.0 12.71133923948451 0.0 80-81 0.0 0.0 0.0 13.858492912210892 0.0 82-83 0.0 0.0 0.0 14.739115545027946 0.0 84-85 0.0 0.0 0.0 15.943754306285443 0.0 86-87 0.0 0.0 0.0 17.135652578063528 0.0 88-89 0.0 0.0 0.0 18.25049636947012 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGTACG 25 0.005038279 56.94641 1 GACCAGT 25 0.0050750673 56.841846 6 CGTGATC 35 2.82486E-4 54.13509 7 CATATAG 55 8.31591E-7 51.6955 3 GGATATG 55 8.339375E-7 51.674404 9 GTCCTAC 50 2.8403414E-5 47.45534 1 ACGTGAT 40 5.45941E-4 47.368202 6 GTAAGCG 115 0.0 43.421978 94-95 TAGAGCC 25 0.0016069469 38.04203 88-89 AAGGACA 25 0.0016077555 38.038136 74-75 GACAAGA 25 0.0016093738 38.030346 78-79 GTGATCT 50 0.0016371049 37.894566 8 CATTCCG 80 1.0970392E-5 35.52615 9 GGCATTC 80 1.0970392E-5 35.52615 7 GCTTTGC 755 0.0 34.570114 1 GACAATG 55 0.0026135568 34.449604 7 CTTTGCG 775 0.0 33.629868 2 CCTTTAG 85 1.6408243E-5 33.497887 1 CCTCATC 85 1.6569627E-5 33.450027 3 TAGGCAT 85 1.6569627E-5 33.450027 5 >>END_MODULE