##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938975_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 490562 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.979333906825232 33.0 31.0 34.0 30.0 34.0 2 32.1853241792067 33.0 31.0 34.0 30.0 34.0 3 32.28181962728463 34.0 31.0 34.0 30.0 34.0 4 35.762739470240255 37.0 35.0 37.0 35.0 37.0 5 35.70956168639234 37.0 35.0 37.0 33.0 37.0 6 35.841706043272815 37.0 35.0 37.0 35.0 37.0 7 35.808295383662006 37.0 35.0 37.0 33.0 37.0 8 35.84307793917996 37.0 35.0 37.0 35.0 37.0 9 37.38934528153424 39.0 37.0 39.0 34.0 39.0 10-11 37.288431431704865 39.0 37.0 39.0 33.5 39.0 12-13 37.26614474826831 39.0 37.0 39.0 33.5 39.0 14-15 38.22038294853658 40.0 37.5 41.0 33.5 41.0 16-17 38.44268410516918 40.0 38.0 41.0 34.0 41.0 18-19 38.55178142620097 40.0 38.0 41.0 34.0 41.0 20-21 38.508029566089505 40.0 38.0 41.0 34.0 41.0 22-23 38.46201295656818 40.0 38.0 41.0 34.0 41.0 24-25 38.27798015337511 40.0 37.5 41.0 33.5 41.0 26-27 37.942194870373164 40.0 37.0 41.0 33.5 41.0 28-29 37.835895360831046 40.0 37.0 41.0 33.0 41.0 30-31 37.847957037030994 40.0 37.0 41.0 33.0 41.0 32-33 37.70574769346179 40.0 36.0 41.0 33.0 41.0 34-35 37.501002931331826 39.5 36.0 41.0 33.0 41.0 36-37 37.293899649789424 39.5 35.5 41.0 32.0 41.0 38-39 37.07691076765016 39.0 35.0 41.0 31.0 41.0 40-41 37.13619481329577 39.0 35.0 41.0 31.5 41.0 42-43 36.976937675563946 39.0 35.0 41.0 31.0 41.0 44-45 36.817921078273486 39.0 35.0 41.0 31.0 41.0 46-47 36.57015219279113 39.0 35.0 41.0 30.5 41.0 48-49 36.20419131526698 39.0 35.0 40.0 30.0 41.0 50-51 35.971411360847355 39.0 35.0 40.0 29.0 41.0 52-53 35.828930899662026 39.0 35.0 40.0 28.0 41.0 54-55 35.689959678898894 38.5 35.0 40.0 28.0 41.0 56-57 35.55501649128958 38.5 34.5 40.0 28.0 41.0 58-59 35.35808521654755 38.0 35.0 40.0 28.0 41.0 60-61 34.7713897529772 38.0 34.0 40.0 26.0 41.0 62-63 34.4267146660361 37.5 34.0 40.0 26.0 41.0 64-65 33.960828600666176 37.0 33.0 39.0 26.0 40.5 66-67 33.33469673558082 36.0 32.5 39.0 24.5 40.0 68-69 32.787198152323256 36.0 32.0 38.0 23.5 39.0 70-71 32.01806907179928 35.0 31.5 37.0 21.5 39.0 72-73 31.352116552036236 34.5 30.5 36.5 20.5 38.5 74-75 30.909417158279687 34.0 30.0 36.0 20.0 37.0 76-77 28.94698631365658 32.0 28.0 34.0 16.0 36.0 78-79 29.930560255380563 34.0 29.0 35.0 18.0 36.0 80-81 29.89045421373853 34.0 29.5 35.0 14.5 36.0 82-83 29.488095286630436 34.0 29.5 35.0 9.0 35.5 84-85 29.032184514903314 34.0 29.0 35.0 4.5 35.0 86-87 28.56144075570468 33.0 29.0 35.0 2.0 35.0 88-89 27.742385264247943 33.0 26.5 35.0 2.0 35.0 90-91 27.050625608995396 32.0 25.0 34.0 2.0 35.0 92-93 26.76932579368153 32.0 25.0 34.0 2.0 35.0 94-95 26.210757253925088 32.0 25.0 34.0 2.0 35.0 96-97 25.193511727365756 31.0 21.5 34.0 2.0 35.0 98-99 24.100358364488077 31.0 10.0 34.0 2.0 35.0 100-101 18.564854187646006 23.5 2.0 30.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0010648728561477583 1101 2 0.08949376705876944 1101 3 0.25055696302533903 1101 4 0.15709744172810503 1101 5 0.20031163515780293 1101 6 0.1634069736905417 1101 7 0.27215797242956086 1101 8 0.18851904515587137 1101 9 0.18886331132836176 1101 10-11 0.29133072402083826 1101 12-13 0.2859698947225411 1101 14-15 0.3478517840450408 1101 16-17 0.395573204654454 1101 18-19 0.46516799339017467 1101 20-21 0.6009077718063338 1101 22-23 0.4864587928288131 1101 24-25 0.4625489449653841 1101 26-27 0.3290229292123783 1101 28-29 0.3438324309319043 1101 30-31 0.3010357828366139 1101 32-33 0.4047573049459601 1101 34-35 0.32888786003962167 1101 36-37 0.4085061927013456 1101 38-39 0.3649886302255183 1101 40-41 0.4856412961655181 1101 42-43 0.6915892214526878 1101 44-45 0.30251028589011497 1101 46-47 0.46148948582811045 1101 48-49 -0.27845897814135867 1101 50-51 -0.3114286419809744 1101 52-53 -0.2917079860525149 1101 54-55 0.14374215593415585 1101 56-57 0.08910407484150795 1101 58-59 0.1595605784236156 1101 60-61 0.4009928546919497 1101 62-63 0.056283496782974396 1101 64-65 0.447447043950298 1101 66-67 0.4781676585879566 1101 68-69 0.5469543172138884 1101 70-71 1.0001498792585792 1101 72-73 0.48239932017632015 1101 74-75 0.8649059760216744 1101 76-77 0.5661175101072615 1101 78-79 0.834028946960288 1101 80-81 0.9112815179699503 1101 82-83 1.0690107292441127 1101 84-85 1.1636232438985132 1101 86-87 1.3602447241321904 1101 88-89 1.6756812895892566 1101 90-91 1.6608225889333852 1101 92-93 1.3106555105941418 1101 94-95 1.5680995243955138 1101 96-97 1.8353246935543837 1101 98-99 1.8210037679388016 1101 100-101 1.4536111677851444 1102 1 0.1526258994382026 1102 2 0.1321168014395866 1102 3 0.20173569738321362 1102 4 0.17041650281840504 1102 5 0.2621512253405385 1102 6 0.18236866800967988 1102 7 0.21860138043730615 1102 8 0.35599362272296275 1102 9 0.3049731056551437 1102 10-11 0.26400702445155133 1102 12-13 0.26123813643317106 1102 14-15 0.4876025295148807 1102 16-17 0.5212989227266789 1102 18-19 0.4891275213867061 1102 20-21 0.5373657069185995 1102 22-23 0.3660214505243502 1102 24-25 0.41482358369923134 1102 26-27 0.20661805626082952 1102 28-29 0.20059772899240613 1102 30-31 0.1739822364390804 1102 32-33 0.5371145188002302 1102 34-35 0.5903757413761141 1102 36-37 0.386402056299481 1102 38-39 0.8052185152829949 1102 40-41 0.6797259611500976 1102 42-43 0.8740088763702332 1102 44-45 1.0606849679504151 1102 46-47 1.140165225563564 1102 48-49 1.2095166571008917 1102 50-51 0.9544505027038852 1102 52-53 1.0506754785344796 1102 54-55 1.266271911012275 1102 56-57 1.1420948743419856 1102 58-59 1.1512062190179506 1102 60-61 0.9662119081364011 1102 62-63 1.173843452946791 1102 64-65 1.3265203675988957 1102 66-67 1.1731927624440104 1102 68-69 0.9684977583536281 1102 70-71 1.1285032192183166 1102 72-73 1.0533196249906496 1102 74-75 1.1022227943309275 1102 76-77 0.8047341219787079 1102 78-79 1.0518131812289617 1102 80-81 1.140674692779445 1102 82-83 1.2438304147450445 1102 84-85 1.48791154320784 1102 86-87 1.4586136106423702 1102 88-89 1.0063971954943938 1102 90-91 1.3739683442153883 1102 92-93 1.485949238453049 1102 94-95 1.4563504100391675 1102 96-97 1.7880937635648344 1102 98-99 2.0190387859065346 1102 100-101 2.4260835790296333 1103 1 0.02723859309137211 1103 2 0.0013321937434369602 1103 3 -0.07537574689464321 1103 4 0.09335503355303842 1103 5 -0.01790874467445036 1103 6 0.026146778954206695 1103 7 0.08529303461021698 1103 8 0.06356483711576288 1103 9 -0.09884998280062973 1103 10-11 0.011095979973795522 1103 12-13 -0.10033107895912963 1103 14-15 0.057617522018624356 1103 16-17 0.04561176630488717 1103 18-19 0.1325183259843996 1103 20-21 0.14041418268072192 1103 22-23 0.035849036731256945 1103 24-25 0.16606046525844675 1103 26-27 0.025383673452232358 1103 28-29 -0.019854544870661073 1103 30-31 0.2998692929108415 1103 32-33 0.32395859676124417 1103 34-35 0.16316434707326977 1103 36-37 0.3407124011270639 1103 38-39 0.01172776066029968 1103 40-41 0.16358403211460626 1103 42-43 0.4929244185991237 1103 44-45 0.5312122043322276 1103 46-47 0.5241232465530743 1103 48-49 0.5272577865361683 1103 50-51 0.34014515537754875 1103 52-53 0.33696983135806136 1103 54-55 0.5192579180087762 1103 56-57 0.8766775829876607 1103 58-59 0.2263686378085623 1103 60-61 0.3512194043882815 1103 62-63 0.42506784075288806 1103 64-65 0.4557932311671138 1103 66-67 0.59820525619714 1103 68-69 0.5332528807924106 1103 70-71 0.49043475345120413 1103 72-73 0.7202861417322808 1103 74-75 0.813941934760706 1103 76-77 0.683619679200099 1103 78-79 1.0304113821285128 1103 80-81 0.992861099576043 1103 82-83 0.6112092253397492 1103 84-85 0.7473068455566647 1103 86-87 0.9611123612670589 1103 88-89 1.0101578151845487 1103 90-91 0.735487584595198 1103 92-93 1.1600746757344105 1103 94-95 1.138072049219577 1103 96-97 0.7281112548192077 1103 98-99 0.9083941082453677 1103 100-101 0.7843544435973477 1104 1 0.07909253624966439 1104 2 0.0957233098736836 1104 3 0.09033101011645783 1104 4 0.21733395318413073 1104 5 0.011472019488920182 1104 6 0.10243136393444274 1104 7 0.202524354015452 1104 8 0.13963297187954993 1104 9 0.18120836464902368 1104 10-11 0.36035722597416964 1104 12-13 0.14884076369096988 1104 14-15 0.31163596734142374 1104 16-17 0.2370102229207376 1104 18-19 0.25492092338700445 1104 20-21 0.2034576609415879 1104 22-23 0.17968020556241981 1104 24-25 0.1045086113152891 1104 26-27 0.09142922210647697 1104 28-29 0.0046655003015558805 1104 30-31 -0.09047514525968126 1104 32-33 0.14733992557736286 1104 34-35 0.29111465763227073 1104 36-37 0.3540240894387523 1104 38-39 0.5208327860696684 1104 40-41 0.5570434712262298 1104 42-43 0.5610591823860602 1104 44-45 0.36184932083890686 1104 46-47 0.7486291754068262 1104 48-49 0.8485075512641913 1104 50-51 0.6968598175269918 1104 52-53 0.6855911375880623 1104 54-55 0.627534786068253 1104 56-57 0.6884600034657282 1104 58-59 0.8631557637261125 1104 60-61 1.1329905318115792 1104 62-63 0.8605280327348197 1104 64-65 0.7989938711068874 1104 66-67 0.8179681020469403 1104 68-69 0.9051002363354215 1104 70-71 0.7932815443414682 1104 72-73 0.8787935763266717 1104 74-75 0.6926168830008734 1104 76-77 0.5692586778825763 1104 78-79 1.01204605180666 1104 80-81 1.0397790367113764 1104 82-83 1.2395312656183108 1104 84-85 1.2210881367292608 1104 86-87 1.2649802325262343 1104 88-89 1.2274973596980097 1104 90-91 0.9990392503207772 1104 92-93 1.1135802281530083 1104 94-95 1.4597688306989696 1104 96-97 1.6524242606539516 1104 98-99 2.5401464139107155 1104 100-101 2.2944146732228 1105 1 0.041980149351868334 1105 2 0.06104482669734068 1105 3 0.08056752610404772 1105 4 0.0157472709107509 1105 5 0.05016365689300528 1105 6 -0.04739306056809056 1105 7 0.028388761424920972 1105 8 0.053758903172862915 1105 9 0.006645445450914167 1105 10-11 0.1357227760178361 1105 12-13 0.21694826420190338 1105 14-15 0.15218740445937584 1105 16-17 0.061230351861894405 1105 18-19 0.3431406435809734 1105 20-21 0.1895091630875143 1105 22-23 0.2114928135552887 1105 24-25 0.09457791254339298 1105 26-27 0.027613567089105118 1105 28-29 0.17646473111366134 1105 30-31 0.1270851913993667 1105 32-33 0.09264211980659098 1105 34-35 0.21539424071013258 1105 36-37 0.30305145692105384 1105 38-39 0.10533197786499926 1105 40-41 0.265160818828555 1105 42-43 -0.05227991056676018 1105 44-45 -0.19247225172300375 1105 46-47 8.72640096638122E-4 1105 48-49 0.12543841725085514 1105 50-51 -0.010913810847490879 1105 52-53 0.07342719280609344 1105 54-55 -0.06106023836368735 1105 56-57 0.0861849537657875 1105 58-59 0.22762820115993065 1105 60-61 0.03155155590908265 1105 62-63 0.41551381948026744 1105 64-65 -0.010567097252369706 1105 66-67 0.07479603805942503 1105 68-69 0.3538117537164638 1105 70-71 0.4104898010347071 1105 72-73 0.27649065750014756 1105 74-75 0.5206875962647217 1105 76-77 0.2565717224917563 1105 78-79 0.39960806061330345 1105 80-81 0.41030371946781585 1105 82-83 0.2310683408463703 1105 84-85 0.28198682316442714 1105 86-87 0.31446186677181487 1105 88-89 0.5042192072353089 1105 90-91 -0.19416346970597687 1105 92-93 0.08938518142577223 1105 94-95 0.3706294293278063 1105 96-97 1.2648018687397133 1105 98-99 1.2144213521341527 1105 100-101 1.3539756415820499 1106 1 0.09602577844606586 1106 2 0.14462738825144328 1106 3 0.07341143846004883 1106 4 -0.1927776895590938 1106 5 0.11115304022257533 1106 6 0.1626227514942542 1106 7 0.2099383435347022 1106 8 0.19214607281370633 1106 9 0.33357564648999016 1106 10-11 0.33254906921864347 1106 12-13 0.15468036268846674 1106 14-15 0.17166786526805566 1106 16-17 -0.17976580806582376 1106 18-19 0.06541306282651504 1106 20-21 0.18608923988895754 1106 22-23 0.16032272094177813 1106 24-25 0.02916115175730738 1106 26-27 0.10348435995108929 1106 28-29 0.24111799517510235 1106 30-31 0.09977775973006686 1106 32-33 0.39083502696719563 1106 34-35 0.08863345462474825 1106 36-37 -0.13534023045188093 1106 38-39 0.18393599864656807 1106 40-41 0.31639867742353545 1106 42-43 0.40310748496067816 1106 44-45 0.407859801586234 1106 46-47 0.5081507065073012 1106 48-49 0.5096376492363959 1106 50-51 0.38912684874139103 1106 52-53 0.5879652546990286 1106 54-55 0.7306881239584371 1106 56-57 0.6815746081592877 1106 58-59 0.59945788265982 1106 60-61 0.6798235233814083 1106 62-63 0.47110903068423937 1106 64-65 0.5472463204119649 1106 66-67 0.5881594896067455 1106 68-69 0.46946569543771943 1106 70-71 0.6712013438402167 1106 72-73 0.5991877435629434 1106 74-75 0.7325918203442328 1106 76-77 0.36149153274701007 1106 78-79 0.35802007778068656 1106 80-81 0.30406247257375796 1106 82-83 0.32781105600336247 1106 84-85 0.637627211689388 1106 86-87 0.7393274642418888 1106 88-89 0.7503294398985076 1106 90-91 1.2480276303617899 1106 92-93 0.9519625475879572 1106 94-95 0.7831864656955503 1106 96-97 0.6212577079084518 1106 98-99 0.8115977695726002 1106 100-101 0.5551095923387699 1107 1 0.03219350783112773 1107 2 0.06851369539506891 1107 3 -0.09793623256556572 1107 4 -0.10016340230090748 1107 5 -0.09645530301745708 1107 6 -0.12726809471778466 1107 7 0.0794855174242528 1107 8 0.012100682129606355 1107 9 -0.10147028455939733 1107 10-11 -0.022081861290381255 1107 12-13 -0.12941776764763802 1107 14-15 -0.029008672071185515 1107 16-17 0.10208440267663832 1107 18-19 0.00916103645668187 1107 20-21 0.20807778414487643 1107 22-23 0.11659852338423349 1107 24-25 0.2335164959699938 1107 26-27 0.014451613814699726 1107 28-29 0.08370094124664718 1107 30-31 0.046115163815230176 1107 32-33 0.2237108510242578 1107 34-35 0.12266349893470618 1107 36-37 0.2653017235089976 1107 38-39 0.26231922570806177 1107 40-41 -0.015560687106884075 1107 42-43 0.1668597815131463 1107 44-45 0.08651753421349184 1107 46-47 0.2864301834337013 1107 48-49 0.13739081037332568 1107 50-51 -0.27365495175574495 1107 52-53 -0.32504025702689887 1107 54-55 -0.3502023980344333 1107 56-57 -0.3192424241545595 1107 58-59 -0.15578474135401876 1107 60-61 -0.21724769676340117 1107 62-63 -0.2669953678891943 1107 64-65 -0.3269739324789924 1107 66-67 -0.07240897467510621 1107 68-69 0.126603920878118 1107 70-71 0.12155474273787092 1107 72-73 0.1460784817019274 1107 74-75 0.028614731832398377 1107 76-77 0.1784460339898466 1107 78-79 0.03904677182585914 1107 80-81 0.2708429761942064 1107 82-83 0.26848759334260563 1107 84-85 0.2559801782948128 1107 86-87 0.36249616628446546 1107 88-89 0.22072606801911832 1107 90-91 0.45421805508890145 1107 92-93 0.40497022077503075 1107 94-95 0.7616302136691786 1107 96-97 1.0349700174296892 1107 98-99 1.3123758955857845 1107 100-101 1.6714091708538454 1108 1 -0.00304754589041778 1108 2 0.04926976342013489 1108 3 0.2199791397645896 1108 4 0.21539145124162928 1108 5 0.09810760612450764 1108 6 0.3209628324659093 1108 7 0.21700986850096626 1108 8 0.366073198319782 1108 9 0.4343718678125299 1108 10-11 0.38916175477869785 1108 12-13 0.27139876124896745 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2316 70-71 -1.111956550896629 2316 72-73 -1.1640616843546745 2316 74-75 -1.8056232826305987 2316 76-77 -1.7489890164520752 2316 78-79 -1.6376320961323572 2316 80-81 -1.4460519439022157 2316 82-83 -1.369442948573294 2316 84-85 -2.123562719660729 2316 86-87 -2.022365899602505 2316 88-89 -1.9104943587284922 2316 90-91 -2.247990676274366 2316 92-93 -2.0927514112221104 2316 94-95 -1.9834496899108558 2316 96-97 -1.9894974408329666 2316 98-99 -2.0498667613198513 2316 100-101 -1.684341208576562 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 12.0 5 40.0 6 98.0 7 155.0 8 194.0 9 322.0 10 504.0 11 710.0 12 1036.0 13 1390.0 14 1754.0 15 2177.0 16 2880.0 17 3867.0 18 4002.0 19 3043.0 20 3054.0 21 3306.0 22 3803.0 23 4425.0 24 4911.0 25 5904.0 26 6935.0 27 8080.0 28 9910.0 29 12085.0 30 14556.0 31 17499.0 32 21913.0 33 28008.0 34 39431.0 35 55977.0 36 78741.0 37 101300.0 38 46860.0 39 1676.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.75558666077305 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15.798866806438758 37.022140999937825 29.26468804561313 17.914304148010284 20-21 20.816940570203155 28.51178852010551 25.111300916092155 25.55996999359918 22-23 17.61907771087039 28.50557116123956 28.345346765546452 25.5300043623436 24-25 25.075525621633965 28.797990875771056 26.595415054570065 19.531068448024918 26-27 23.271370387433187 23.155890590791785 35.75786546858501 17.814873553190015 28-29 16.85678874433813 34.20627769782413 30.632009817311573 18.30492374052617 30-31 18.97303500882661 29.176128603520045 28.221509207806555 23.629327179846786 32-33 19.651236744794744 30.11138245522482 30.303712884406046 19.933667915574382 34-35 24.25721127136725 26.064552563254395 28.759356533492898 20.918879631885456 36-37 21.896481943825687 25.06027560094647 34.780933234513626 18.26230922071421 38-39 21.221761706621436 28.0804993955308 32.9741515515389 17.723587346308864 40-41 18.731111049952958 30.545846698937783 31.6619829715319 19.061059279577357 42-43 16.96061353232634 31.9854893570611 26.486204390498973 24.56769272011359 44-45 20.43077826294353 27.01221476126991 27.459973825511565 25.097033150274996 46-47 23.884604055206914 30.418026947056852 27.524056960031963 18.17331203770427 48-49 22.067781062073184 27.805422484965852 29.68800326164509 20.43879319131587 50-51 16.179823609212765 30.271720905865457 35.08021979030024 18.468235694621537 52-53 19.344139986382967 33.030279556916355 27.550136374199386 20.075444082501296 54-55 15.732262180927181 27.19666423408254 31.321932803600767 25.749140781389507 56-57 22.214113608473546 27.25333393128697 27.684879790933664 22.847672669305815 58-59 26.088405628703697 26.086978679337975 27.36623878570686 20.458376906251466 60-61 19.978575199825503 28.222026977716986 33.483945669479134 18.315452152978374 62-63 19.425265565469456 28.288659625690798 31.856440739812093 20.429634069027657 64-65 18.131995817033967 33.60947975888829 29.208294177482514 19.050230246595223 66-67 19.64199441036712 27.775195647343498 26.980578981594167 25.602230960695216 68-69 16.579689286901004 29.69886800299257 29.77643415846327 23.94500855164316 70-71 23.295730203317827 29.050354491379277 25.9139517532952 21.739963552007698 72-73 20.398644819615054 24.445737745687598 32.859964693555554 22.29565274114179 74-75 16.962178073311833 34.27232439528541 27.819521283752103 20.945976247650655 76-77 20.160348322504365 26.399528279270097 28.107985689233566 25.33213770899197 78-79 19.47483276373284 27.21361904072686 31.356430607778847 21.955117587761457 80-81 22.34702239472279 27.622298506610786 28.44920723578263 21.581471862883795 82-83 19.35575931278819 26.48574492113127 32.42108031196872 21.737415454111815 84-85 18.521957513948315 28.027605129637294 30.47358024792024 22.97685710849415 86-87 18.99965212565507 29.630834929032613 29.05883157066797 22.310681374644346 88-89 19.038835736534008 28.161001309161776 26.83873767734843 25.961425276955787 90-91 19.833621680132513 24.321702947889882 29.66408194710311 26.180593424874488 92-93 21.779102335688457 29.306693139705075 29.257973507935798 19.656231016670674 94-95 19.631929353919414 27.794878485316723 29.55858430603517 23.0146078547287 96-97 14.57685267101814 28.121419922456287 32.6574418727908 24.644285533734777 98-99 19.1170535019019 29.640901659729046 26.3527342109662 24.889310627402857 100-101 18.432237508638107 25.977507292700775 29.27099492619644 26.31926027246468 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8228.0 1 4919.0 2 1400.5 3 1084.5 4 777.5 5 727.5 6 1298.5 7 2209.0 8 2774.0 9 2681.0 10 1935.5 11 1196.0 12 1009.5 13 908.0 14 773.0 15 662.5 16 627.0 17 603.5 18 567.0 19 618.0 20 682.5 21 781.0 22 871.5 23 901.0 24 945.0 25 1211.5 26 1847.5 27 2430.0 28 2607.0 29 2892.0 30 3550.5 31 4305.0 32 5636.0 33 7466.5 34 9086.0 35 9991.0 36 11057.0 37 12041.0 38 11152.5 39 9629.5 40 8609.5 41 7561.0 42 6448.5 43 6278.0 44 6631.0 45 7426.0 46 13193.5 47 40607.5 48 98763.0 49 97425.0 50 42568.5 51 16270.5 52 4860.5 53 1377.5 54 674.5 55 445.0 56 322.0 57 220.0 58 172.0 59 155.5 60 129.5 61 115.5 62 102.5 63 80.5 64 54.0 65 30.5 66 15.5 67 10.0 68 11.0 69 12.5 70 7.0 71 3.5 72 2.0 73 2.5 74 2.5 75 0.5 76 1.5 77 1.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38690318451082634 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 6.115434950118436E-4 16-17 0.002344250064212067 18-19 3.057717475059218E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.012740489479413407 36-37 0.021709794072920445 38-39 0.009988543751860111 40-41 0.006217358865953743 42-43 0.00519811970760067 44-45 0.0016307826533649163 46-47 0.001528858737529609 48-49 0.0024461739800473743 50-51 7.134674108471508E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0010192391583530725 60-61 0.0018346304850355307 62-63 0.0010192391583530725 64-65 2.0384783167061454E-4 66-67 0.00224232614837676 68-69 0.0026500218117179886 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.08999881768257631 78-79 0.002751945727553296 80-81 0.0 82-83 0.0 84-85 2.0384783167061454E-4 86-87 0.09020266551424692 88-89 0.42430926162238414 90-91 0.37844349949649586 92-93 0.0 94-95 8.153913266824582E-4 96-97 0.0 98-99 0.0 100-101 6.115434950118436E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 490562.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 12.834667177645231 #Duplication Level Percentage of deduplicated Percentage of total 1 69.295765699946 8.89388089578891 2 10.63498618214161 2.7299301617328697 3 4.947428607731648 1.9049579869618927 4 2.8636320320193134 1.470150562008472 5 1.8757345700581303 1.2037214460149788 6 1.3722562815666592 1.0567471593804656 7 0.9863092023760364 0.8861265242721614 8 0.8258949842762301 0.8480069797497565 9 0.6638925065912773 0.7668755427448518 >10 5.454083415393412 14.21797856336202 >50 0.6321273148883454 5.443960192595431 >100 0.33194625329563865 7.235782632980133 >500 0.031765191702931926 2.5955944406619347 >1k 0.05876560465042407 18.358739568087216 >5k 0.020647374606905754 20.055365071081738 >10k+ 0.004764778755439789 12.332182272577166 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 31216 6.363313913429902 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 17377 3.5422637709402682 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 11904 2.4266045882069953 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 9099 1.8548114203709214 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 8865 1.8071110277599975 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 8690 1.77143765721764 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 8203 1.6721637631940507 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 8177 1.6668637195706149 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 8139 1.6591175019671316 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 7742 1.5781899127938976 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 7642 1.557805129626836 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 7461 1.520908672094455 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 6472 1.319303166572217 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6305 1.2852605786832245 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 5886 1.1998483372132371 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5703 1.1625441840175146 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 4913 1.001504396997729 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4833 0.9851965704640799 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 4788 0.9760234180389022 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 4616 0.9409615909915566 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 4386 0.8940765897073153 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 4324 0.8814380241437372 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3578 0.7293675417174588 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 3314 0.6755517141564165 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3105 0.6329475173372581 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3012 0.6139896689918909 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2884 0.5878971465380523 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2873 0.5856548203896755 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2847 0.5803547767662396 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2701 0.5505929933423298 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2680 0.546312188877247 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2663 0.5428467757388464 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2537 0.5171619489483491 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 2365 0.4821001219010033 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2237 0.4560075994471647 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1922 0.3917955324709211 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1824 0.37181844496720085 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1711 0.34878363998842143 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1708 0.3481720964934096 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1566 0.3192257043961823 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1560 0.31800261740615865 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1535 0.3129064216143933 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1514 0.3086256171493104 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1514 0.3086256171493104 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1400 0.28538696433886035 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1303 0.2656137246668107 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1259 0.2566444200733037 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1180 0.24054044137132516 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1122 0.2287172671344295 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1104 0.22504800616435844 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 1084 0.22097104953094615 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1079 0.21995181037259307 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1020 0.20792478830402683 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 918 0.18713230947362414 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 878 0.17897839620679953 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 830 0.16919370028661004 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 767 0.15635128689136132 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 746 0.1520704824262784 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 679 0.13841267770434726 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 663 0.13515111239761743 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 645 0.13148185142754637 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 644 0.13127800359587577 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 609 0.12414332948740424 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 582 0.11863943803229765 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 570 0.11619326405225028 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 549 0.11191245958716736 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT 539 0.10987398127046122 No Hit TACCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 537 0.10946628560712 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 527 0.10742780729041386 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 523 0.10661241596373139 No Hit GCTTTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 519 0.10579702463704894 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 507 0.10335085065700156 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 501 0.10212776366697787 No Hit TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA 497 0.10131237234029541 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.038478316706145E-4 0.0 16-17 0.0 0.0 0.0 3.057717475059218E-4 0.0 18-19 0.0 0.0 0.0 6.115434950118436E-4 0.0 20-21 0.0 0.0 0.0 0.0017327065692002233 0.0 22-23 0.0 0.0 0.0 0.004178880549247597 0.0 24-25 0.0 0.0 0.0 0.008357761098495196 0.0 26-27 0.0 0.0 0.0 0.013657804721931174 0.0 28-29 0.0 0.0 0.0 0.04240034898748782 0.0 30-31 0.0 0.0 0.0 0.10100660059278949 0.0 32-33 0.0 0.0 0.0 0.21414214716998056 0.0 34-35 0.0 0.0 0.0 0.38486470619412017 0.0 36-37 0.0 0.0 0.0 0.6040011252400308 0.0 38-39 0.0 0.0 0.0 0.8834765024604434 0.0 40-41 0.0 0.0 0.0 1.1772212278977987 0.0 42-43 0.0 0.0 0.0 1.4921661278288982 0.0 44-45 0.0 0.0 0.0 1.8517537028958624 0.0 46-47 0.0 0.0 0.0 2.2953265846111197 0.0 48-49 0.0 0.0 0.0 2.6909952258837824 0.0 50-51 0.0 0.0 0.0 3.0899254324631746 0.0 52-53 0.0 0.0 0.0 3.471732421182236 0.0 54-55 0.0 0.0 0.0 3.8904358674336783 0.0 56-57 0.0 0.0 0.0 4.493315829599521 0.0 58-59 0.0 0.0 0.0 5.0315760291257785 0.0 60-61 0.0 0.0 0.0 5.721295167583303 0.0 62-63 0.0 0.0 0.0 6.355465771910584 0.0 64-65 0.0 0.0 0.0 6.89637599324856 0.0 66-67 0.0 0.0 0.0 7.571316163909964 0.0 68-69 0.0 0.0 0.0 8.383751697033198 0.0 70-71 0.0 0.0 0.0 9.189766023458809 0.0 72-73 0.0 0.0 0.0 9.943595304976741 0.0 74-75 0.0 0.0 0.0 10.871001015162202 0.0 76-77 0.0 0.0 0.0 11.596495448077919 0.0 78-79 0.0 0.0 0.0 12.800522665840404 0.0 80-81 0.0 0.0 0.0 13.944618621091728 0.0 82-83 0.0 0.0 0.0 14.82768742788883 0.0 84-85 0.0 0.0 0.0 16.035078134873878 0.0 86-87 0.0 0.0 0.0 17.229116808884505 0.0 88-89 0.0 0.0 0.0 18.34620292643947 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGAACCG 15 6.6408707E-4 94.90909 5 GAACCGA 15 6.6408707E-4 94.90909 6 AACCGAT 20 0.0020812552 71.18181 7 ACCGATT 20 0.0020812552 71.18181 8 AGCCCAG 20 5.413285E-4 47.45454 94-95 GTAAGCG 235 0.0 42.406185 94-95 ACTCCCG 45 9.6651085E-4 42.181812 6 GCTTATG 60 8.147204E-5 39.695152 1 TACTCCC 50 0.0016225268 37.963634 5 CTCCGAC 85 0.0 36.28877 94-95 CCACTGT 140 0.0 33.896103 94-95 GTACATA 75 3.0254057E-4 31.75612 1 AACCGAA 30 0.003967252 31.639587 44-45 GCTTTGC 740 0.0 30.254143 1 TCAACGA 120 0.0 29.65909 94-95 CTTTGCG 755 0.0 29.541239 2 TTGCGTT 795 0.0 28.054884 4 GCTTTCC 85 6.297135E-4 28.020107 1 TTTGCGT 805 0.0 27.706377 3 AAGCAGG 375 0.0 27.20727 94-95 >>END_MODULE