##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938974_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 496284 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.736805941759155 33.0 31.0 34.0 30.0 34.0 2 31.681434420614003 33.0 31.0 34.0 30.0 34.0 3 31.764465507652876 33.0 31.0 34.0 30.0 34.0 4 35.21834675306881 37.0 35.0 37.0 33.0 37.0 5 35.296007930942764 37.0 35.0 37.0 33.0 37.0 6 35.48301577322662 37.0 35.0 37.0 33.0 37.0 7 35.44054815387963 37.0 35.0 37.0 33.0 37.0 8 35.47313433437306 37.0 35.0 37.0 33.0 37.0 9 36.949621587639335 39.0 37.0 39.0 33.0 39.0 10-11 36.85758960595143 39.0 37.0 39.0 32.5 39.0 12-13 36.80826401818314 39.0 37.0 39.0 32.5 39.0 14-15 37.73873326563016 40.0 37.0 41.0 32.0 41.0 16-17 37.75043624215167 40.0 37.0 41.0 32.5 41.0 18-19 37.78692442230658 40.0 37.0 41.0 33.0 41.0 20-21 37.5962604073474 40.0 37.0 41.0 32.0 41.0 22-23 37.761248599592165 40.0 37.0 41.0 32.5 41.0 24-25 37.59367015660388 40.0 37.0 41.0 32.0 41.0 26-27 37.38160206655866 39.5 36.5 41.0 31.5 41.0 28-29 37.337337089247285 39.5 36.5 41.0 32.0 41.0 30-31 37.34671276930145 40.0 36.0 41.0 32.0 41.0 32-33 37.132974264735516 40.0 36.0 41.0 31.0 41.0 34-35 36.73034895342183 39.0 35.0 41.0 30.5 41.0 36-37 36.355273391848215 39.0 35.0 41.0 30.0 41.0 38-39 36.08336154298748 38.5 35.0 40.0 30.0 41.0 40-41 36.16773258859846 39.0 35.0 40.0 30.0 41.0 42-43 36.047823423684825 39.0 35.0 40.0 30.0 41.0 44-45 36.01383079043451 39.0 35.0 41.0 30.0 41.0 46-47 35.74594284724069 39.0 35.0 41.0 28.0 41.0 48-49 35.481578088352634 39.0 35.0 41.0 27.0 41.0 50-51 34.24991033359931 38.0 34.0 39.5 24.5 40.5 52-53 34.53997912485593 38.0 33.5 39.5 25.0 40.5 54-55 35.216321702895925 39.0 35.0 40.0 26.5 41.0 56-57 35.0990068186764 39.0 35.0 40.5 25.5 41.0 58-59 34.95551438289367 39.0 35.0 40.0 26.0 41.0 60-61 34.4745689967841 38.0 34.0 40.0 23.0 41.0 62-63 34.1912997396652 38.0 34.0 40.0 23.0 41.0 64-65 33.714042765835686 37.0 33.0 39.5 22.0 41.0 66-67 33.09614152380492 36.5 33.0 39.0 21.5 40.5 68-69 32.64154899210936 36.0 32.0 39.0 21.0 40.0 70-71 31.851268628446615 35.0 31.5 37.5 18.5 39.0 72-73 31.295334929193768 35.0 31.0 37.0 15.5 39.0 74-75 30.882232753826436 35.0 31.0 37.0 11.5 38.0 76-77 30.4326111661871 34.5 31.0 36.0 7.5 37.0 78-79 29.907260761983057 34.0 30.0 35.5 2.0 37.0 80-81 29.369696585019867 34.0 29.0 35.0 2.0 36.0 82-83 28.919000008059903 34.0 29.0 35.0 2.0 36.0 84-85 28.504250590387763 34.0 29.0 35.0 2.0 35.5 86-87 28.260386190165306 34.0 29.0 35.0 2.0 35.0 88-89 27.63825954493798 34.0 26.5 35.0 2.0 35.0 90-91 27.023168185958042 33.0 25.0 35.0 2.0 35.0 92-93 26.547935254813776 33.0 25.0 35.0 2.0 35.0 94-95 25.925479161125484 33.0 23.5 35.0 2.0 35.0 96-97 25.201498537127936 32.0 19.5 35.0 2.0 35.0 98-99 24.538045756059027 32.0 7.5 35.0 2.0 35.0 100-101 21.901497932635344 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19167343532336645 1101 2 0.13194212732818045 1101 3 0.048492976464743265 1101 4 -0.05396173287277861 1101 5 0.027714506400712935 1101 6 0.016776289727772564 1101 7 0.07116715010125318 1101 8 0.10960148827693672 1101 9 -0.07993296271270367 1101 10-11 0.2873579719380288 1101 12-13 0.18617837385934877 1101 14-15 0.28259018249197965 1101 16-17 0.4562317975439001 1101 18-19 0.2879808717060186 1101 20-21 0.3702668661733881 1101 22-23 0.5014345708831769 1101 24-25 0.6278337712198052 1101 26-27 0.3304239652883467 1101 28-29 0.4095651255345274 1101 30-31 0.48680578898854776 1101 32-33 0.3322722431974441 1101 34-35 0.6250299648198734 1101 36-37 0.4852623014800912 1101 38-39 0.5590859836568285 1101 40-41 0.6638511769024262 1101 42-43 0.5359275131459427 1101 44-45 0.5568339499721446 1101 46-47 0.7627313924579724 1101 48-49 0.7348433189056536 1101 50-51 0.5750322194430524 1101 52-53 0.7132322703625391 1101 54-55 0.762590031212639 1101 56-57 0.539916258769253 1101 58-59 0.7166090049877525 1101 60-61 0.5480371929767713 1101 62-63 0.7365917533831237 1101 64-65 0.6538727032313219 1101 66-67 0.5434889984415392 1101 68-69 0.34260022676300395 1101 70-71 0.4400923050907686 1101 72-73 0.2602115908782565 1101 74-75 0.12511641545745888 1101 76-77 0.20279507678388242 1101 78-79 0.09300242119337554 1101 80-81 0.1366308770693827 1101 82-83 0.1429511035338571 1101 84-85 0.010744478164344429 1101 86-87 0.18102854293137938 1101 88-89 0.05236760745438218 1101 90-91 0.4391730441918078 1101 92-93 0.6529168888822205 1101 94-95 0.5425276408106541 1101 96-97 0.5917184897450731 1101 98-99 0.9965688078542598 1101 100-101 0.9617358185582567 1102 1 0.3063406255845109 1102 2 0.26585141458519956 1102 3 0.2996890226879181 1102 4 0.35242580047044925 1102 5 0.3608642624024938 1102 6 0.5722206346679783 1102 7 0.5997166178068127 1102 8 0.2744810695290667 1102 9 0.4431522858110952 1102 10-11 0.5143170214224924 1102 12-13 0.4470960370316561 1102 14-15 0.6658900728244745 1102 16-17 0.4976109262379964 1102 18-19 0.6751646198443026 1102 20-21 0.9928789640791678 1102 22-23 0.717630401602527 1102 24-25 0.6560073044768302 1102 26-27 1.0863467287178281 1102 28-29 0.8503566471351931 1102 30-31 0.919154349662449 1102 32-33 0.8906402566831915 1102 34-35 0.5888229279016741 1102 36-37 0.7028205952644839 1102 38-39 0.867390543238848 1102 40-41 0.7626692581521581 1102 42-43 1.0241250870517788 1102 44-45 1.014322228958605 1102 46-47 1.0429845376165616 1102 48-49 1.1789895166998505 1102 50-51 1.1749014804564908 1102 52-53 1.2658367537998743 1102 54-55 1.4037857422076456 1102 56-57 1.1911951542403187 1102 58-59 1.09556290153634 1102 60-61 1.5865429373509272 1102 62-63 1.5082223510949504 1102 64-65 1.4448148380600898 1102 66-67 1.3387483932110271 1102 68-69 1.1954901330427887 1102 70-71 1.4370010224598992 1102 72-73 1.3374745007545599 1102 74-75 1.3744830736636082 1102 76-77 1.4368777248948739 1102 78-79 1.1583910703675144 1102 80-81 1.0512722068716478 1102 82-83 0.9822163791632512 1102 84-85 1.0343205965094668 1102 86-87 1.1581899265617075 1102 88-89 1.1641207071177178 1102 90-91 1.556656151086493 1102 92-93 1.3275587933613515 1102 94-95 1.0312759467111512 1102 96-97 1.1731717517546194 1102 98-99 0.8135007038569029 1102 100-101 1.1996759851187697 1103 1 -0.04277415460248335 1103 2 0.2855450688521586 1103 3 0.19541794019193404 1103 4 0.200174785221094 1103 5 0.29932341226155046 1103 6 0.282099484003524 1103 7 0.003744622678063081 1103 8 0.18986482650966252 1103 9 0.33681916757639385 1103 10-11 0.33260800620224273 1103 12-13 0.33133577658441027 1103 14-15 0.21898870605867415 1103 16-17 0.3798225929936123 1103 18-19 0.5917225242337807 1103 20-21 0.4570262273938539 1103 22-23 0.4062533013584826 1103 24-25 0.5312323089647961 1103 26-27 0.33744931135693434 1103 28-29 0.4071548150770852 1103 30-31 0.5163344551416458 1103 32-33 0.49674048617564637 1103 34-35 0.48189060634054215 1103 36-37 0.41537038736277054 1103 38-39 0.6652625223564925 1103 40-41 0.6869003614140539 1103 42-43 0.7965151562876613 1103 44-45 1.1179140526230071 1103 46-47 0.8764011610209081 1103 48-49 1.0727097688019072 1103 50-51 0.7457554543632554 1103 52-53 0.8699331697391699 1103 54-55 1.0953901030493398 1103 56-57 1.0784575955935907 1103 58-59 1.0354363653197183 1103 60-61 0.8794005307368877 1103 62-63 0.9231403305910391 1103 64-65 0.5758802555374203 1103 66-67 0.6355699470641198 1103 68-69 0.5550218325968999 1103 70-71 0.9161380726328652 1103 72-73 0.7693424140229084 1103 74-75 1.0391912106992258 1103 76-77 1.3407286372850358 1103 78-79 1.2368648728099387 1103 80-81 1.0026081634378983 1103 82-83 1.0302690896485807 1103 84-85 0.9840845540654932 1103 86-87 1.1796059871515965 1103 88-89 1.273265471730273 1103 90-91 1.1229639426704772 1103 92-93 1.3151307826678753 1103 94-95 0.8088574941598061 1103 96-97 0.7068557120276857 1103 98-99 0.6414662751378408 1103 100-101 0.6743299270033241 1104 1 0.08435331057393824 1104 2 0.13688735538858054 1104 3 0.02190838018804797 1104 4 0.21897558647571458 1104 5 0.1449616171181134 1104 6 0.17867657237746215 1104 7 0.1127883447185809 1104 8 0.13257419591487007 1104 9 0.17145631303741737 1104 10-11 0.14683906545899816 1104 12-13 0.3372110269764903 1104 14-15 0.39311123509952495 1104 16-17 0.22973076329260067 1104 18-19 0.36335107887591533 1104 20-21 0.6731244134681234 1104 22-23 0.40452773569214884 1104 24-25 0.36126177774038126 1104 26-27 0.6954324813089627 1104 28-29 0.5022413478003784 1104 30-31 0.6524343991648465 1104 32-33 0.5526316968935845 1104 34-35 0.5514383045839821 1104 36-37 0.529098118082004 1104 38-39 0.7038263705992378 1104 40-41 0.6443533709763685 1104 42-43 0.8143328939924217 1104 44-45 0.7946295563447734 1104 46-47 0.7093590026297107 1104 48-49 0.9628032639282296 1104 50-51 1.092577961661057 1104 52-53 0.7871846628392163 1104 54-55 0.9719288866412015 1104 56-57 1.103998743164155 1104 58-59 0.9251921492030206 1104 60-61 0.8090201198394524 1104 62-63 0.76001423476432 1104 64-65 0.7558549040632911 1104 66-67 1.2588484293065179 1104 68-69 1.1376084231474621 1104 70-71 0.7577353080268558 1104 72-73 0.8195040990632236 1104 74-75 0.7990692645572253 1104 76-77 0.7438957210790633 1104 78-79 0.6379455487875632 1104 80-81 0.5811283405281209 1104 82-83 0.4417378559847833 1104 84-85 0.5416943911776464 1104 86-87 0.3489288529784531 1104 88-89 0.32245103249285023 1104 90-91 0.2693617665147414 1104 92-93 0.4096760521660734 1104 94-95 -0.041379135019372626 1104 96-97 0.47784354054438793 1104 98-99 0.8072871216365165 1104 100-101 1.4009341710621506 1105 1 -0.03762968981437709 1105 2 0.16381059825866728 1105 3 0.405327286862768 1105 4 0.08454490088223565 1105 5 0.24697223108886135 1105 6 0.068537210078631 1105 7 0.3643394514191911 1105 8 0.2043738248573561 1105 9 0.3209838619005154 1105 10-11 0.12244634854136649 1105 12-13 0.24941062140199222 1105 14-15 0.04492984837992964 1105 16-17 0.25803899990648915 1105 18-19 0.04891003818902817 1105 20-21 0.17093905729605297 1105 22-23 0.27692493981692934 1105 24-25 0.31999888780639196 1105 26-27 0.16063289551270543 1105 28-29 0.36457901013804417 1105 30-31 0.041447213592945786 1105 32-33 0.0022131785348662447 1105 34-35 0.3767974968867023 1105 36-37 0.39849234511741827 1105 38-39 0.22571444929794637 1105 40-41 0.1977274055796414 1105 42-43 0.14939998636848628 1105 44-45 0.17701511098946554 1105 46-47 0.33005408827496296 1105 48-49 0.20918545316755655 1105 50-51 0.07433358145653557 1105 52-53 -0.003105279776306702 1105 54-55 0.17860360726940883 1105 56-57 -0.15347202527006232 1105 58-59 -0.10414296750750651 1105 60-61 0.5313051437856089 1105 62-63 0.28733226557071845 1105 64-65 0.35314100666500536 1105 66-67 0.19529620080412968 1105 68-69 0.14444709942134892 1105 70-71 0.09930022151867846 1105 72-73 0.5565839627211631 1105 74-75 0.41839230032544705 1105 76-77 0.29481473561934024 1105 78-79 0.44840419313324986 1105 80-81 0.425646612953372 1105 82-83 0.4735236781360541 1105 84-85 0.44814475144860566 1105 86-87 0.8257744326479575 1105 88-89 0.9140483056515869 1105 90-91 1.0009771610869471 1105 92-93 1.1843600060859067 1105 94-95 0.8921044909891727 1105 96-97 0.5646170660222332 1105 98-99 0.9557899477140914 1105 100-101 1.4225525858699335 1106 1 0.1491922184361023 1106 2 0.2829454316792166 1106 3 0.3064261879616659 1106 4 0.14913586547319824 1106 5 0.2867507065816213 1106 6 0.23320666760523778 1106 7 0.23856760421877254 1106 8 0.10518491852559464 1106 9 0.5150532845413665 1106 10-11 0.2606854903519391 1106 12-13 0.37681773809482877 1106 14-15 0.46023597082891143 1106 16-17 0.5164508261754577 1106 18-19 0.5589745768250012 1106 20-21 0.5815399643255361 1106 22-23 0.43163015523642656 1106 24-25 0.44504685571150304 1106 26-27 0.6788737816107329 1106 28-29 0.47883419939323346 1106 30-31 0.5528766571652497 1106 32-33 0.5456590184092747 1106 34-35 0.5499136664314364 1106 36-37 0.492457110510756 1106 38-39 0.547827653554009 1106 40-41 0.7266476916427891 1106 42-43 0.7433909160737002 1106 44-45 0.5648225416540384 1106 46-47 -0.09597068060695335 1106 48-49 0.017776068901035558 1106 50-51 0.03849283490151123 1106 52-53 0.01823171656069178 1106 54-55 0.1365401152059249 1106 56-57 -0.12321299858247414 1106 58-59 0.16336095713927534 1106 60-61 0.25257236862426424 1106 62-63 0.15815033638879328 1106 64-65 0.04728892224149206 1106 66-67 0.2778533262183984 1106 68-69 0.646060565320532 1106 70-71 0.5840507884121031 1106 72-73 0.2997899630700189 1106 74-75 0.3171887419790309 1106 76-77 0.505616235706551 1106 78-79 0.7220174846036862 1106 80-81 0.7186258559326149 1106 82-83 0.49693201583010804 1106 84-85 0.5092907483088851 1106 86-87 0.6569012423461942 1106 88-89 0.5723990818595084 1106 90-91 0.598552601519529 1106 92-93 1.0294629190459652 1106 94-95 0.9162585115717654 1106 96-97 1.097741084860541 1106 98-99 1.1813655625754187 1106 100-101 1.6879546441291353 1107 1 -0.052912280838210535 1107 2 -0.027649633632830017 1107 3 0.12605205475734493 1107 4 -0.057443718464874394 1107 5 0.015221920901893782 1107 6 -0.19619987972583885 1107 7 -0.3133998203901598 1107 8 -0.11385233353052371 1107 9 -0.05630617361054391 1107 10-11 -0.001429491102662439 1107 12-13 -0.15823709177132628 1107 14-15 -0.23735312770357808 1107 16-17 -0.28525454392613625 1107 18-19 -0.13631650525854155 1107 20-21 -0.21327135642918194 1107 22-23 -0.17817068333799568 1107 24-25 -0.11183913041976012 1107 26-27 0.12184668920627928 1107 28-29 0.01977517665496009 1107 30-31 -0.07675969181072162 1107 32-33 0.14280060847539033 1107 34-35 0.10399768513809704 1107 36-37 -0.24004571161470523 1107 38-39 -0.25758679572836485 1107 40-41 0.233219212365249 1107 42-43 0.1941246686942506 1107 44-45 0.4265787100139846 1107 46-47 0.3108588630150422 1107 48-49 0.32405731386152326 1107 50-51 0.3058892225809089 1107 52-53 0.33602320180500556 1107 54-55 -0.05266323909177828 1107 56-57 0.20078716268184138 1107 58-59 0.1925497976488657 1107 60-61 -0.06868629933251924 1107 62-63 0.1871375965081299 1107 64-65 0.4019494608642944 1107 66-67 0.12485381001133078 1107 68-69 0.16996799960532627 1107 70-71 9.7950958610582E-4 1107 72-73 -0.001290507821771314 1107 74-75 0.026146974522166744 1107 76-77 0.11666687270058773 1107 78-79 0.3105314339666947 1107 80-81 0.038614567430759195 1107 82-83 -0.2372237654954894 1107 84-85 0.27614286893459195 1107 86-87 -0.1728842761185696 1107 88-89 -0.24648093749220834 1107 90-91 -0.15128613416826298 1107 92-93 -0.2663159875820007 1107 94-95 0.06629479604200839 1107 96-97 -0.19676786530878942 1107 98-99 -0.2001656420689919 1107 100-101 -0.04531418347931648 1108 1 0.2550148680087503 1108 2 0.176268081251866 1108 3 0.0827082392437184 1108 4 0.14139013897747077 1108 5 0.2130861766670904 1108 6 0.03924299239155715 1108 7 0.2669329888341565 1108 8 0.21704389288456838 1108 9 0.4378631136011961 1108 10-11 0.20748036214681065 1108 12-13 0.3518978662999501 1108 14-15 0.27273597082891143 1108 16-17 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Count 2 2183.0 3 449.0 4 381.0 5 394.0 6 425.0 7 500.0 8 668.0 9 827.0 10 1083.0 11 1443.0 12 1937.0 13 2898.0 14 2890.0 15 2658.0 16 3250.0 17 4115.0 18 3798.0 19 3024.0 20 3141.0 21 3556.0 22 4035.0 23 4377.0 24 5149.0 25 6040.0 26 6959.0 27 8247.0 28 9675.0 29 11326.0 30 13561.0 31 16345.0 32 20239.0 33 25050.0 34 33763.0 35 53056.0 36 68215.0 37 93622.0 38 69462.0 39 7541.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.87269641125121 19.368735855156803 15.090729065632072 15.66783866795991 2 13.082174513699114 27.73220930349814 33.15659443161535 26.029021751187393 3 29.44937345835258 25.581817485080183 32.24637315783848 12.722435898728765 4 11.208360733117363 16.568767070233548 44.498392321874505 27.724479874774584 5 9.358610228024594 43.29582681104459 35.4424532412413 11.903109719689512 6 23.310960705383145 38.90618823662281 16.28798573744968 21.494865320544363 7 21.019766034691408 37.488503428801934 23.535901573215007 17.95582896329165 8 20.8477707545914 24.812128643978234 21.798522845076217 32.541577756354144 9 18.30910634796096 26.425734726107663 29.199047190684897 26.066111735246484 10-11 23.99824132562098 23.59215292399784 27.386412223593666 25.023193526787512 12-13 32.999467535820315 23.726926552213758 25.228515877113722 18.0450900348522 14-15 24.66241532017722 27.34796460819603 32.83877728235886 15.150842789267887 16-17 16.62663189591363 30.146888279795654 33.055711862595025 20.1707679616957 18-19 15.784250543851527 36.84373484048535 29.539937410429644 17.832077205233478 20-21 20.714020606911465 28.647257925269688 25.054663987929548 25.5840574798893 22-23 17.271914818072513 28.245261170242813 28.803168096317876 25.6796559153668 24-25 24.97018927540267 28.35317688503653 26.49633696307909 20.18029687648171 26-27 23.20861635683307 22.862178442764602 35.97203880831007 17.957166392092258 28-29 16.96137384081677 33.654621147698585 30.849480946169916 18.534524065314738 30-31 18.70842340020557 29.042468526608516 28.66037988971074 23.588728183475173 32-33 19.664305108953073 29.880294845053037 30.422034301760277 20.033365744233617 34-35 24.08700585655239 25.93458581907288 28.631370934874045 21.34703738950068 36-37 21.731054525998346 24.819320816511826 34.95822997227492 18.49139468521491 38-39 21.51446655069245 27.82337606547511 32.6853772184628 17.97678016536964 40-41 18.698929261583462 30.420942395650606 32.049736873022496 18.830391469743436 42-43 16.88282479946023 31.82275911116154 26.408033342586158 24.886382746792073 44-45 20.070524219901813 27.056475106267637 27.76992486536055 25.10307580847 46-47 23.785009989827802 30.206969779251146 27.624305473939316 18.383714756981735 48-49 21.718373944193637 27.61589169086091 29.851591753183254 20.8141426117622 50-51 16.173797262872462 30.07567328757268 35.27481725123977 18.475712198315083 52-53 19.20992178055387 32.76785777619247 27.874050377011855 20.148170066241807 54-55 15.679002963751046 26.889277300706745 31.852775033563844 25.57894470197837 56-57 22.02094608899302 27.120312884610126 27.739335758371396 23.11940526802546 58-59 25.85409137319093 25.818436704860382 27.796359172163427 20.53111274978526 60-61 20.2627625499589 27.450769984571966 33.838118994302555 18.44834847116658 62-63 19.547223792532616 27.8448452281813 32.20574915963066 20.402181819655425 64-65 18.348403495223558 32.93109074730572 29.68568527177978 19.034820485690947 66-67 19.637155709440762 27.408437513303348 27.391713308615294 25.562693468640596 68-69 16.377638779504615 29.123753399359202 30.52424377877989 23.974364042356296 70-71 23.274583754384935 28.344636267163448 26.28020802398499 22.100571954466627 72-73 20.255806057657715 23.795436233448363 33.55843050213594 22.39032720675798 74-75 17.018441044600653 33.42991673355549 28.550884148566148 21.000758073277716 76-77 19.98167943262986 25.791670424342033 28.79348728149329 25.433162861534814 78-79 19.486512415750088 26.516143868916096 32.04247218853745 21.95487152679636 80-81 22.17623151761221 27.228320549133066 28.967458030949516 21.62798990230521 82-83 19.279468040806773 25.678196299264535 33.08706343408717 21.955272225841522 84-85 18.805507678995006 27.28193873218815 30.983683465513174 22.928870123303675 86-87 19.29298076932819 28.780760615065393 29.930183573332574 21.99607504227384 88-89 19.41362618922887 27.21235328748509 27.56791866074767 25.806101862538366 90-91 19.88931239301904 23.45012521016153 30.341193889784655 26.319368507034774 92-93 22.13155793032712 28.208171340309 30.12705388235962 19.53321684700426 94-95 19.99945270843447 26.62593736349383 30.13245469388665 23.24215523418505 96-97 15.193933736899554 26.679152693363655 33.25093771884752 24.875975850889276 98-99 19.65861168345872 28.63328120550089 27.276403524152283 24.43170358688811 100-101 18.826047129968142 24.491735637681277 29.97604591402469 26.70617131832589 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9319.0 1 5347.0 2 1270.0 3 1039.0 4 775.5 5 811.0 6 1331.5 7 2204.5 8 2825.0 9 2680.5 10 1932.0 11 1240.5 12 1039.0 13 991.0 14 883.0 15 883.5 16 1088.5 17 1036.0 18 791.5 19 683.0 20 688.0 21 792.5 22 868.5 23 945.0 24 1052.5 25 1271.0 26 1813.5 27 2431.0 28 2628.5 29 2808.0 30 3547.5 31 4439.0 32 5711.5 33 7535.5 34 9084.0 35 9896.5 36 11070.5 37 12139.5 38 11157.0 39 9650.0 40 8691.0 41 7692.0 42 6446.0 43 6108.0 44 6716.5 45 7647.5 46 14659.5 47 39936.5 48 96289.5 49 96006.0 50 40780.5 51 15705.0 52 6223.0 53 3471.0 54 2203.5 55 1334.0 56 782.0 57 486.5 58 364.5 59 280.5 60 210.5 61 186.0 62 172.5 63 136.0 64 108.0 65 94.0 66 86.0 67 79.5 68 64.0 69 50.0 70 40.0 71 36.0 72 33.0 73 27.5 74 22.0 75 12.5 76 10.0 77 9.5 78 9.5 79 9.5 80 5.5 81 5.5 82 3.0 83 2.5 84 4.0 85 1.5 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2829025316149624 2 0.09047239080848869 3 0.09409934634201385 4 0.10840567094647419 5 0.11042064624287706 6 0.08865891304172611 7 0.09752480434589872 8 0.09389784881237356 9 0.09812929693481959 10-11 0.0918828735159707 12-13 0.09591282410877643 14-15 0.10467796664812888 16-17 0.09369635128273328 18-19 0.10367047899992746 20-21 0.10639069565007134 22-23 0.11928653754704967 24-25 0.1323838769736683 26-27 0.1347010985645316 28-29 0.12543221220107842 30-31 0.11837979866366839 32-33 0.11404760177640223 34-35 0.37891610448855895 36-37 0.577894915008342 38-39 0.5608683737537378 40-41 0.5251025622425869 42-43 0.5523047287440256 44-45 0.571547742824673 46-47 0.5605661274592774 48-49 0.5806151316584859 50-51 0.573663466885896 52-53 0.577189673654601 54-55 0.5685252798800687 56-57 0.5407186207897091 58-59 0.5359834288431624 60-61 0.47916112548460155 62-63 0.5543197040404285 64-65 0.5893802741978383 66-67 0.6018731210355361 68-69 0.6029813574485576 70-71 0.5995558994446728 72-73 0.592704983436903 74-75 0.5899847667867592 76-77 0.5741672107099967 78-79 0.5515994873902845 80-81 0.5452523152066155 82-83 0.5616743638722989 84-85 0.5697342650579104 86-87 0.5592563935166155 88-89 0.5731597230617952 90-91 0.587063052606975 92-93 0.5944177124388456 94-95 0.5935109735554642 96-97 0.5908915056701405 98-99 0.5935109735554642 100-101 0.572252984178414 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 496284.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 14.846740978955598 #Duplication Level Percentage of deduplicated Percentage of total 1 72.91333025705057 10.825253282394758 2 9.539643332157107 2.8326522716831493 3 4.359273635351918 1.9416301956138016 4 2.525718628701718 1.4999476106422935 5 1.553975190684292 1.1535733571906408 6 1.2662522732824841 1.1279831709263244 7 0.925599196547325 0.9619492065027282 8 0.7383078635216199 0.8769172489945274 9 0.5523737140685648 0.7380854510723699 >10 4.713498547813577 14.100192631638336 >50 0.5523737140685648 5.626012525086443 >100 0.2673651638120572 7.062689911421686 >500 0.023072120735050623 2.3498641906650226 >1k 0.04478705789745121 15.461106946828831 >5k 0.02035775358975055 21.177390365194125 >10k+ 0.00407155071795011 12.264751634144966 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 29069 5.857331689113492 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 18881 3.8044748571382514 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 12918 2.6029450878932225 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 9512 1.9166445019384062 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 8704 1.7538344979890548 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 8036 1.619234148189343 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 7784 1.568456770719991 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 7562 1.5237243191398473 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 7524 1.5160674130135163 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 7314 1.4737529317890563 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 7175 1.4457447751690564 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6934 1.3971838705257473 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 6480 1.3057039920690572 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6446 1.2988530760612875 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5779 1.1644542237912163 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 5533 1.1148858314997057 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 5221 1.0520186022519364 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 5096 1.0268314110469006 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 4986 1.0046666827864692 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 4398 0.8861861353579805 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 4388 0.8841711600615776 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 4149 0.8360132504775492 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 3357 0.6764272070024421 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3303 0.6655463404018667 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3017 0.6079180469247447 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 3002 0.6048955839801404 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2958 0.5960296926759678 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2916 0.5875667964310758 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2838 0.5718499891191334 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2764 0.5569391719257522 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2584 0.5206696165905006 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2546 0.5130127104641697 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2385 0.48057160819208355 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2236 0.4505484762756809 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 2103 0.42374930483352274 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1918 0.3864722618500697 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1917 0.38627076432042945 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1696 0.34173981026992606 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1680 0.3385158497956815 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1665 0.3354933868510772 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1542 0.31070919070532194 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1514 0.3050672598753939 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1461 0.29438789080445876 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1359 0.2738351427811495 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1333 0.268596207010502 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1252 0.25227490710963885 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1251 0.2520734095799985 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1076 0.21681134189294837 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1062 0.21399037647798438 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1046 0.21076641600373977 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1029 0.2073409579998549 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 985 0.1984750666956823 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 939 0.18920618033222913 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 808 0.16281000394935158 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 804 0.16200401383079044 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 794 0.15998903853438756 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 701 0.14124976827784091 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 675 0.13601083250719348 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 672 0.1354063399182726 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 646 0.13016740414762515 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 645 0.12996590661798485 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 614 0.12371948319913598 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 612 0.1233164881398554 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 595 0.11989103013597052 No Hit TACCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 558 0.11243562153927993 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 550 0.11082364130215765 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 536 0.10800267588719362 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 528 0.10639069565007134 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.014975296402866E-4 0.0 14-15 0.0 0.0 0.0 2.014975296402866E-4 0.0 16-17 0.0 0.0 0.0 3.022462944604299E-4 0.0 18-19 0.0 0.0 0.0 0.001611980237122293 0.0 20-21 0.0 0.0 0.0 0.0028209654149640127 0.0 22-23 0.0 0.0 0.0 0.005138187005827309 0.0 24-25 0.0 0.0 0.0 0.008261398715251752 0.0 26-27 0.0 0.0 0.0 0.015616058547122212 0.0 28-29 0.0 0.0 0.0 0.04352346640230191 0.0 30-31 0.0 0.0 0.0 0.10427497158884833 0.0 32-33 0.0 0.0 0.0 0.223561509135898 0.0 34-35 0.0 0.0 0.0 0.41619314747201197 0.0 36-37 0.0 0.0 0.0 0.6544639762716509 0.0 38-39 0.0 0.0 0.0 0.9570125170265413 0.0 40-41 0.0 0.0 0.0 1.2561355997775467 0.0 42-43 0.0 0.0 0.0 1.5842743267967534 0.0 44-45 0.0 0.0 0.0 1.9592612294573268 0.0 46-47 0.0 0.0 0.0 2.433787911760202 0.0 48-49 0.0 0.0 0.0 2.8480668327006313 0.0 50-51 0.0 0.0 0.0 3.2689951721191894 0.0 52-53 0.0 0.0 0.0 3.643780577250123 0.0 54-55 0.0 0.0 0.0 4.069746354909689 0.0 56-57 0.0 0.0 0.0 4.676656914186232 0.0 58-59 0.0 0.0 0.0 5.213345584383136 0.0 60-61 0.0 0.0 0.0 5.911030780762628 0.0 62-63 0.0 0.0 0.0 6.525799743695142 0.0 64-65 0.0 0.0 0.0 7.094022777280751 0.0 66-67 0.0 0.0 0.0 7.757151147326934 0.0 68-69 0.0 0.0 0.0 8.552562645581965 0.0 70-71 0.0 0.0 0.0 9.3509966067816 0.0 72-73 0.0 0.0 0.0 10.105000362695552 0.0 74-75 0.0 0.0 0.0 11.023829097855259 0.0 76-77 0.0 0.0 0.0 11.735518372544753 0.0 78-79 0.0 0.0 0.0 12.8941291679764 0.0 80-81 0.0 0.0 0.0 14.051127983170925 0.0 82-83 0.0 0.0 0.0 14.938321606177109 0.0 84-85 0.0 0.0 0.0 16.13612367112379 0.0 86-87 0.0 0.0 0.0 17.319518662701196 0.0 88-89 0.0 0.0 0.0 18.46321864093946 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTCAG 25 0.0050616106 56.880074 1 GTAGGGG 55 8.388124E-7 51.630936 6 GGGATGA 75 1.3988574E-7 44.177586 7 ATATGAG 80 2.3242501E-7 41.41231 3 GGATATG 70 4.367974E-6 40.628624 1 TAAGAGG 50 0.0016439047 37.862686 4 GTATAGT 90 5.783859E-7 36.86671 1 GATTGGC 90 5.843631E-7 36.81837 9 GGAAAAG 155 1.8189894E-12 36.641308 6 TAGGCAT 65 1.3556606E-4 36.406433 5 AAAGTCA 160 1.8189894E-12 35.50343 9 AGGGGTG 80 1.1018852E-5 35.50343 8 GGCATTC 80 1.1026472E-5 35.499847 7 GTAAGCG 115 0.0 35.15912 94-95 GTATAGG 190 0.0 34.92636 1 AGGTATA 55 0.0026217971 34.42757 8 GGTTTGC 55 0.0026217971 34.42757 8 GAAAAGT 165 1.8189894E-12 34.424095 7 CATTCCG 85 1.6689284E-5 33.41499 9 TCGGCGG 30 0.0039230273 31.712143 94-95 >>END_MODULE