##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938974_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 496284 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.954205656438653 33.0 31.0 34.0 30.0 34.0 2 32.15388366338629 33.0 31.0 34.0 30.0 34.0 3 32.259790764965224 34.0 31.0 34.0 30.0 34.0 4 35.76822343658067 37.0 35.0 37.0 35.0 37.0 5 35.707570665183646 37.0 35.0 37.0 33.0 37.0 6 35.82962174883736 37.0 35.0 37.0 35.0 37.0 7 35.810431124114416 37.0 35.0 37.0 33.0 37.0 8 35.83444962964754 37.0 35.0 37.0 33.0 37.0 9 37.38756437846072 39.0 37.0 39.0 34.0 39.0 10-11 37.29831507765715 39.0 37.0 39.0 33.5 39.0 12-13 37.292997557849944 39.0 37.0 39.0 34.0 39.0 14-15 38.18732923084363 40.0 37.0 41.0 33.0 41.0 16-17 38.265095187433005 40.0 37.5 41.0 33.5 41.0 18-19 38.48706587357239 40.0 38.0 41.0 34.0 41.0 20-21 38.46258694618404 40.0 38.0 41.0 34.0 41.0 22-23 38.438396160263075 40.0 38.0 41.0 34.0 41.0 24-25 38.277008728872985 40.0 37.5 41.0 33.5 41.0 26-27 37.94335501446753 40.0 37.0 41.0 33.0 41.0 28-29 37.826380661073095 40.0 37.0 41.0 33.0 41.0 30-31 37.849896430269766 40.0 37.0 41.0 33.0 41.0 32-33 37.66348300569835 40.0 36.0 41.0 33.0 41.0 34-35 37.52984178413973 39.5 36.0 41.0 33.0 41.0 36-37 37.350471705716885 39.5 35.5 41.0 32.0 41.0 38-39 37.153409136703985 39.0 35.0 41.0 31.5 41.0 40-41 37.21363473333817 39.0 35.5 41.0 32.0 41.0 42-43 37.084955388446936 39.0 35.0 41.0 31.0 41.0 44-45 36.93149486987289 39.0 35.0 41.0 31.0 41.0 46-47 36.68141930829928 39.0 35.0 41.0 31.0 41.0 48-49 36.37868639730477 39.0 35.0 41.0 30.0 41.0 50-51 36.13751904151655 39.0 35.0 40.0 29.0 41.0 52-53 36.04162032223485 39.0 35.0 40.0 29.0 41.0 54-55 35.9515207018562 39.0 35.0 40.0 28.0 41.0 56-57 35.632430221405485 38.5 35.0 40.0 28.0 41.0 58-59 35.42974486382796 38.0 35.0 40.0 28.0 41.0 60-61 34.735888120511646 38.0 34.0 40.0 26.0 41.0 62-63 34.52671051252912 37.5 34.0 40.0 26.0 41.0 64-65 34.23671123792022 37.0 33.5 39.5 26.0 40.5 66-67 33.611636683834256 36.0 33.0 39.0 25.5 40.0 68-69 33.11703278767803 36.0 32.5 38.0 25.0 39.5 70-71 32.41025098532292 35.0 31.5 37.0 23.5 39.0 72-73 31.757366749683648 35.0 31.0 37.0 21.5 38.5 74-75 31.368761636482336 34.5 31.0 36.0 21.5 37.0 76-77 29.33306030418067 32.5 28.5 34.0 19.0 36.0 78-79 30.346293654439798 34.0 30.0 35.0 20.0 36.0 80-81 30.32925703830871 34.0 30.5 35.0 18.0 36.0 82-83 30.014612600849514 34.0 30.0 35.0 16.0 36.0 84-85 29.628893939760296 34.0 30.0 35.0 10.0 35.0 86-87 29.271456464443744 34.0 29.0 35.0 4.5 35.0 88-89 28.600255095872527 33.5 29.0 35.0 2.0 35.0 90-91 28.044968203689823 33.0 27.5 35.0 2.0 35.0 92-93 27.835718056596626 33.0 27.0 35.0 2.0 35.0 94-95 27.442407976078215 33.0 27.0 35.0 2.0 35.0 96-97 26.71381406613955 32.5 25.0 34.0 2.0 35.0 98-99 25.93754785566329 32.0 24.5 34.0 2.0 35.0 100-101 21.62454562307066 27.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.12299404942494974 1101 2 0.10200320038595834 1101 3 0.20432230741646862 1101 4 0.2768649429362071 1101 5 0.13393867979792873 1101 6 0.16139131564923304 1101 7 0.020403800950624884 1101 8 0.22390105112143033 1101 9 0.3560323004602637 1101 10-11 0.26253525046509907 1101 12-13 0.40891499947620247 1101 14-15 0.35494707691314176 1101 16-17 0.4173543500003163 1101 18-19 0.17473328149362288 1101 20-21 0.3549173213003769 1101 22-23 0.23500592944889576 1101 24-25 0.14723289715104215 1101 26-27 0.30638775057961 1101 28-29 0.33437192441179064 1101 30-31 0.2804498065543015 1101 32-33 0.5487096186506264 1101 34-35 0.6981208705623416 1101 36-37 0.6384273399848936 1101 38-39 0.7812193888716692 1101 40-41 0.6202902260685406 1101 42-43 0.6757210897319155 1101 44-45 0.8479717698731406 1101 46-47 0.5988214243946572 1101 48-49 0.6011104862971806 1101 50-51 0.546150231670083 1101 52-53 0.5845891050673515 1101 54-55 0.5452061236563139 1101 56-57 0.2348991775994449 1101 58-59 0.4517689028214562 1101 60-61 0.3783992858264611 1101 62-63 0.36248903692685275 1101 64-65 0.7339140714269021 1101 66-67 0.4147837914976975 1101 68-69 0.16479029604520434 1101 70-71 0.23610537607678594 1101 72-73 0.2420020999868484 1101 74-75 0.29138999093715867 1101 76-77 0.28637016250758407 1101 78-79 0.48733827315852096 1101 80-81 0.5596138462996265 1101 82-83 0.6478529507988675 1101 84-85 0.8525934328656888 1101 86-87 0.6710277572592034 1101 88-89 0.8561067904986679 1101 90-91 0.9686565140000916 1101 92-93 0.9738497516325779 1101 94-95 0.9632889297047527 1101 96-97 0.24953831798564607 1101 98-99 0.4243025525656243 1101 100-101 1.1638879459448894 1102 1 0.25680276823077364 1102 2 0.2558729307188372 1102 3 0.23499089605797963 1102 4 0.08335208693586083 1102 5 0.12599856112715457 1102 6 0.32143882226998954 1102 7 0.05657970953869551 1102 8 0.1933426088788508 1102 9 0.08631250332119578 1102 10-11 0.24192590143031367 1102 12-13 0.35883909424042315 1102 14-15 0.47499228482644895 1102 16-17 0.23631437643544473 1102 18-19 0.021914730924457615 1102 20-21 0.18806707730215777 1102 22-23 0.3704931835949452 1102 24-25 0.31053690600107586 1102 26-27 0.47075251714107935 1102 28-29 0.29247558654009964 1102 30-31 0.19832608820343012 1102 32-33 0.00677975796187269 1102 34-35 0.22620820228977578 1102 36-37 0.7029827077062478 1102 38-39 0.5559173243450317 1102 40-41 0.642726568633428 1102 42-43 0.6009460517792959 1102 44-45 0.319283421925455 1102 46-47 0.5274130350702393 1102 48-49 0.46307070908024883 1102 50-51 0.5768858659457266 1102 52-53 0.6214187085882088 1102 54-55 0.3640152974146531 1102 56-57 0.6270730365066939 1102 58-59 0.6372865669515662 1102 60-61 0.34057597022828645 1102 62-63 0.46187700606670745 1102 64-65 0.4366888028851861 1102 66-67 0.6655584080206154 1102 68-69 0.8170533256458512 1102 70-71 0.6928944648787265 1102 72-73 0.6992030405413132 1102 74-75 0.9003798526794853 1102 76-77 0.4724361641454138 1102 78-79 0.7699164651293273 1102 80-81 0.8011672936424645 1102 82-83 0.9808757989931856 1102 84-85 0.9132146585556562 1102 86-87 0.982478672671558 1102 88-89 1.532446386213497 1102 90-91 1.6349272921604587 1102 92-93 1.5344063261753504 1102 94-95 1.088096772128356 1102 96-97 1.3297823640938873 1102 98-99 0.9721132731625204 1102 100-101 2.0146515478279134 1103 1 0.11133948944838679 1103 2 0.1035297303423306 1103 3 -0.03218586164554438 1103 4 -0.046192275943617744 1103 5 -0.10192673980876776 1103 6 -0.037534914672470165 1103 7 0.044818828054800974 1103 8 0.0020390626625683694 1103 9 -0.1610832668370179 1103 10-11 -0.024986430513770586 1103 12-13 -0.04850757520797089 1103 14-15 -0.0639365239961549 1103 16-17 0.11412784225660033 1103 18-19 -0.001876309651557051 1103 20-21 0.0751039076863762 1103 22-23 0.1273524848316825 1103 24-25 0.2792352034873318 1103 26-27 0.14186752051311657 1103 28-29 0.09870972705935088 1103 30-31 -0.09431802383546284 1103 32-33 -0.06880719444633598 1103 34-35 -0.033096430740641836 1103 36-37 0.19872763631129686 1103 38-39 0.31665567869496414 1103 40-41 0.40597818066018476 1103 42-43 0.37077191892597483 1103 44-45 0.37484436842412094 1103 46-47 0.5154459500124773 1103 48-49 0.4996269638411235 1103 50-51 0.5131762393925285 1103 52-53 0.48077088973683146 1103 54-55 0.7752749829178214 1103 56-57 0.5651731495547665 1103 58-59 0.5208810106143247 1103 60-61 0.7021866869919151 1103 62-63 0.4449003871260615 1103 64-65 0.558259152266821 1103 66-67 0.6337108725172129 1103 68-69 0.36847807104508945 1103 70-71 0.47517745381166065 1103 72-73 0.8642095662779354 1103 74-75 0.41136201243146076 1103 76-77 0.32894758782340716 1103 78-79 0.3368380155861317 1103 80-81 0.37628846856616605 1103 82-83 0.40681717895602176 1103 84-85 0.2167511711437804 1103 86-87 0.3608137122383859 1103 88-89 0.8216747966993125 1103 90-91 1.0753174778407093 1103 92-93 0.8100762451979939 1103 94-95 0.18515800731202248 1103 96-97 0.3252386076955105 1103 98-99 0.5698853041949441 1103 100-101 0.627995587574258 1104 1 0.18615872140975043 1104 2 0.20595801296500582 1104 3 0.08834004265727202 1104 4 0.1423940092789735 1104 5 0.08809828783266482 1104 6 0.1187883813892654 1104 7 -0.09713554854412365 1104 8 -0.04251569050752835 1104 9 -0.0071748374718723085 1104 10-11 0.14518996578343746 1104 12-13 0.02322830684290267 1104 14-15 0.032641952232211224 1104 16-17 0.062437204259914836 1104 18-19 -0.19027523452699313 1104 20-21 -0.0976030098426861 1104 22-23 -0.01165501420838666 1104 24-25 -0.11325002742852064 1104 26-27 0.03783857912220867 1104 28-29 0.08771098075450823 1104 30-31 -0.029384941549579935 1104 32-33 0.10475325355307064 1104 34-35 -0.004957682307761502 1104 36-37 0.0624680890294016 1104 38-39 0.09660210751422227 1104 40-41 0.05630363252245729 1104 42-43 -0.03477302211334887 1104 44-45 0.08306416755532098 1104 46-47 0.03646269216299913 1104 48-49 -0.27845974005088436 1104 50-51 -0.16889869549396508 1104 52-53 -0.007571133107248329 1104 54-55 -0.09553196190572066 1104 56-57 -0.11053847280078344 1104 58-59 0.14280402170327733 1104 60-61 0.10366058838948788 1104 62-63 0.04479352233546052 1104 64-65 0.13939080876360066 1104 66-67 0.03687795631124402 1104 68-69 0.17999356181354642 1104 70-71 0.05338536365663771 1104 72-73 0.002224749405336013 1104 74-75 -0.08291308807233122 1104 76-77 -0.10777318974516703 1104 78-79 0.1373686385939834 1104 80-81 0.07046549402404167 1104 82-83 0.20794226627519308 1104 84-85 -0.15487692088021987 1104 86-87 -0.024998251991981135 1104 88-89 0.17476577980912467 1104 90-91 0.5065562304371554 1104 92-93 0.1847182944113115 1104 94-95 0.6334997525852195 1104 96-97 0.8577305928362158 1104 98-99 1.286066761534613 1104 100-101 1.8739048866615136 1105 1 -0.17707206773465956 1105 2 -0.0033831442166842862 1105 3 -0.012251060591644602 1105 4 -0.09381430068349061 1105 5 -0.23839349281193734 1105 6 -0.08025808709242455 1105 7 -0.0024727362733329983 1105 8 0.09381774350032401 1105 9 0.06817141096407653 1105 10-11 0.1610828768590622 1105 12-13 0.12511811139052043 1105 14-15 0.09869018970901777 1105 16-17 0.01854886668387934 1105 18-19 0.12003455535090524 1105 20-21 0.1568281072130091 1105 22-23 -0.007566909621942841 1105 24-25 8.803604560227996E-4 1105 26-27 -0.22762071241682236 1105 28-29 -0.006160098332522068 1105 30-31 -0.1645296971262269 1105 32-33 0.08120620259737876 1105 34-35 0.01393389123497002 1105 36-37 -0.023832985311535992 1105 38-39 -0.08076985607036846 1105 40-41 -0.014438913967602218 1105 42-43 0.0897577627164381 1105 44-45 -0.01991292180050408 1105 46-47 0.0910901245313056 1105 48-49 0.1872346984010278 1105 50-51 -0.04403137012830172 1105 52-53 0.23153228838820183 1105 54-55 0.08727426840881947 1105 56-57 -0.16756699245389228 1105 58-59 -0.11635160672145872 1105 60-61 -0.0218431437587725 1105 62-63 0.08242997226427207 1105 64-65 0.4292902780650607 1105 66-67 0.38677656268825444 1105 68-69 0.3063537464793811 1105 70-71 0.368958992685414 1105 72-73 0.13318647870593736 1105 74-75 0.367466598686093 1105 76-77 0.2791038866667961 1105 78-79 0.48683578274550854 1105 80-81 0.09024372244014245 1105 82-83 0.2819143075229853 1105 84-85 0.4533273333373238 1105 86-87 0.42144649927004707 1105 88-89 0.7990851481484249 1105 90-91 0.8204967749424839 1105 92-93 0.8662991720054762 1105 94-95 0.7560975866448523 1105 96-97 0.8211145510706714 1105 98-99 0.7622802490437799 1105 100-101 1.3640157724534596 1106 1 0.1089736325967543 1106 2 0.03915130546062784 1106 3 -0.085057619112483 1106 4 -0.04856748400810318 1106 5 0.005004379157362848 1106 6 -0.01612063724068946 1106 7 0.10096351002005122 1106 8 0.09033789002047143 1106 9 -0.25058840065288024 1106 10-11 0.13218875510779782 1106 12-13 0.21507973706643213 1106 14-15 0.11461553420578952 1106 16-17 0.07827762998406484 1106 18-19 0.11581337970115158 1106 20-21 0.0391671966949545 1106 22-23 0.26901167052806585 1106 24-25 0.33771535086243887 1106 26-27 0.1845674961999606 1106 28-29 0.41645458856788053 1106 30-31 0.46629186226676467 1106 32-33 0.42051372047632896 1106 34-35 0.49186429416538147 1106 36-37 0.35858895782470057 1106 38-39 0.5052322370745799 1106 40-41 0.5815448641590351 1106 42-43 0.689007719109263 1106 44-45 0.6836541460522767 1106 46-47 0.691774757358786 1106 48-49 0.449361856956763 1106 50-51 0.4818146092891027 1106 52-53 0.8147260787248456 1106 54-55 0.4906451039225175 1106 56-57 0.6612966042668447 1106 58-59 0.9206360088375902 1106 60-61 0.872575136373797 1106 62-63 0.9070723933352625 1106 64-65 0.6503569098459749 1106 66-67 0.5592822316224897 1106 68-69 0.8054859634687332 1106 70-71 0.7500727202277133 1106 72-73 0.568482135430159 1106 74-75 0.8127090546428377 1106 76-77 0.524158831721742 1106 78-79 0.685483959965115 1106 80-81 0.6147771255449754 1106 82-83 0.6306758634273955 1106 84-85 0.5870662135047731 1106 86-87 0.7359633316440224 1106 88-89 0.8120103182164513 1106 90-91 0.9162276313876241 1106 92-93 0.7011369532718312 1106 94-95 1.2127651254552454 1106 96-97 1.1332722346569248 1106 98-99 1.5323308333800796 1106 100-101 2.1882875324395066 1107 1 -0.03963143110264866 1107 2 -0.0362800765745277 1107 3 -0.15375621611296708 1107 4 -0.014218185507857584 1107 5 0.12313048784950809 1107 6 -0.010960179250041335 1107 7 0.04339215056189971 1107 8 -0.07467581744606377 1107 9 -0.04421039710506136 1107 10-11 -0.08737260596299024 1107 12-13 -0.06257709247719134 1107 14-15 -0.04302033097724234 1107 16-17 -0.06760609844683074 1107 18-19 -0.05798923278070589 1107 20-21 -0.004535431913332388 1107 22-23 0.10180073680769652 1107 24-25 -0.0039029365105562874 1107 26-27 -0.10919560652540383 1107 28-29 0.17915415315424355 1107 30-31 0.057446514742167665 1107 32-33 0.0011458765801819482 1107 34-35 -0.3255240302984177 1107 36-37 -0.10014753628916395 1107 38-39 0.058046299322604966 1107 40-41 -0.083005208409908 1107 42-43 -0.4311745095635402 1107 44-45 -0.34976788265277037 1107 46-47 -0.4810401112107954 1107 48-49 -0.7803389977440958 1107 50-51 -0.5010308994998027 1107 52-53 -0.3664140599624659 1107 54-55 -0.5281623464887133 1107 56-57 -0.17987820624759365 1107 58-59 -0.24151525708725785 1107 60-61 -0.375126847177242 1107 62-63 -0.1655570212752835 1107 64-65 -0.2654429611425684 1107 66-67 -0.30104271596660226 1107 68-69 -0.45582269642482487 1107 70-71 -0.4401990101383575 1107 72-73 -0.45711857413281365 1107 74-75 -0.33494858122234916 1107 76-77 -0.48071941372254656 1107 78-79 -0.06580615453254524 1107 80-81 -0.08192501927037554 1107 82-83 -0.21757441878515138 1107 84-85 -0.25882557814173524 1107 86-87 -0.278046505479022 1107 88-89 -0.24092469226572533 1107 90-91 -0.15692589223759512 1107 92-93 0.038078575590812846 1107 94-95 -0.2496804478393848 1107 96-97 -0.5472228467815512 1107 98-99 -0.4074369460103391 1107 100-101 0.21687163177832147 1108 1 -0.15390029902717473 1108 2 -0.06426749795817699 1108 3 0.010027850972981867 1108 4 -0.03777688571750559 1108 5 0.15318813984112722 1108 6 0.09835585848581019 1108 7 -0.030874096817555596 1108 8 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-0.43661608447995803 2316 66-67 -0.6240277590404446 2316 68-69 -0.851809518826748 2316 70-71 -0.17503860635419954 2316 72-73 -0.30114725478746607 2316 74-75 -0.8408767386488449 2316 76-77 -0.6389633402239632 2316 78-79 -0.8561414081308385 2316 80-81 -0.9182796869236007 2316 82-83 -1.0195862927171113 2316 84-85 -1.391510601836746 2316 86-87 -1.353523488725152 2316 88-89 -1.657877636965619 2316 90-91 -2.1765020245773243 2316 92-93 -2.657110191740017 2316 94-95 -3.000024862334744 2316 96-97 -2.7775048967084395 2316 98-99 -4.153325993453219 2316 100-101 -5.082562858999118 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 7.0 5 39.0 6 103.0 7 139.0 8 173.0 9 292.0 10 485.0 11 723.0 12 1032.0 13 1325.0 14 1629.0 15 2184.0 16 2790.0 17 3838.0 18 3691.0 19 2924.0 20 2776.0 21 3067.0 22 3347.0 23 3844.0 24 4489.0 25 5343.0 26 6182.0 27 7500.0 28 9016.0 29 11316.0 30 13544.0 31 16476.0 32 20427.0 33 25840.0 34 35902.0 35 53643.0 36 75919.0 37 110433.0 38 62757.0 39 3088.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.79501864165738 20.059960794864896 15.605990415173885 15.539030148303842 2 13.381450943411433 28.476033883824588 32.84732129184096 25.295193880923023 3 29.699728381330043 25.574872452063737 32.146512883752045 12.578886282854171 4 11.289302093156337 17.129506492250403 43.7019932135632 27.879198201030054 5 9.071491899734022 44.096276295639555 35.25751591843314 11.574715886193278 6 23.133729880471666 39.77641834111114 15.74965140927372 21.340200369143474 7 21.088126959563475 37.250848304599785 23.182492282644613 18.478532453192123 8 21.325894044539016 25.445511038034674 21.13991182468103 32.088683092745285 9 18.070096960611263 26.481006842856107 28.64408282354458 26.804813372988047 10-11 24.223327772001515 23.880580474083388 27.421798808746605 24.47429294516849 12-13 33.236413021576354 23.716964480015477 24.98730565563266 18.05931684277551 14-15 24.269562624174355 27.90693925582033 32.63140820266061 15.192089917344715 16-17 16.738334931931938 30.395068793474493 32.742590696962125 20.12400557763144 18-19 15.809899170636168 37.0643623409177 29.24172449645767 17.88401399198846 20-21 20.8371617863965 28.538901113072352 25.085334203802663 25.538602896728484 22-23 17.586805135769033 28.430092447066603 28.398457334913076 25.58464508225129 24-25 25.061960490364388 28.909455070080842 26.40776249083186 19.62082194872291 26-27 23.32102183427231 23.13463661935505 35.755333639609574 17.789007906763064 28-29 16.98362228079084 34.139323451894484 30.56233930572011 18.31471496159457 30-31 18.991545163656294 29.293106366515946 28.175198072071638 23.540150397756125 32-33 19.738698003562476 30.057386496441552 30.19380032400803 20.01011517598794 34-35 24.235921151395164 26.149103054300355 28.675563078380108 20.939412715924373 36-37 21.929610672255357 25.05793567628567 34.708608737707564 18.303844913751412 38-39 21.201538046084956 28.10148444018105 32.90546014421197 17.79151736952202 40-41 18.78225317317826 30.500494691387132 31.612693922046308 19.104558213388305 42-43 16.923427221958594 32.069932366740915 26.491624175489818 24.515016235810666 44-45 20.38836907756803 27.076925246911777 27.444157084551986 25.090548590968215 46-47 23.820327958700897 30.450127145453592 27.488504519607805 18.241040376237706 48-49 22.122402182630037 27.83215994132368 29.557287993519815 20.488149882526464 50-51 16.11477783229444 30.287206529371996 35.01134942839785 18.58666620993571 52-53 19.292280226644422 32.97436548427916 27.65845765730912 20.074896631767295 54-55 15.786626205962714 27.159146778860492 31.294178333373633 25.76004868180316 56-57 22.13178341433534 27.33132641793812 27.603549580482145 22.9333405872444 58-59 26.02771606897281 26.091691727997663 27.32949479379184 20.55109740923768 60-61 19.994216898544856 28.31583794016807 33.33101606271542 18.358929098571654 62-63 19.44733257570262 28.239475784026887 31.810918748136146 20.502272892134343 64-65 18.100312020496432 33.64723448284895 29.137898551527712 19.11455494512691 66-67 19.683099726764947 27.796327043822387 26.952139535258606 25.56843369415406 68-69 16.595469007860626 29.750882078541764 29.783223279216397 23.870425634381213 70-71 23.240322073651377 29.14752440135084 25.72126040734741 21.89089311765038 72-73 20.482727631759236 24.41112346962626 32.806618790853626 22.29953010776088 74-75 17.023115796600333 34.39371408306534 27.61060199401955 20.972568126314773 76-77 20.145495920788246 26.506201531620952 28.041756441603926 25.306546105986875 78-79 19.492367273577226 27.295459857662145 31.288838648838162 21.923334219922463 80-81 22.27252943878908 27.851089295645238 28.347478459914083 21.528902805651605 82-83 19.3627036132537 26.567147036777328 32.33813703443996 21.732012315529012 84-85 18.507648846225145 28.12824914766545 30.340692023115796 23.02340998299361 86-87 19.089688238754288 29.704140101361 28.967088449225166 22.23908321065955 88-89 19.060417897881365 28.117739206463572 26.924439173271374 25.89740372238369 90-91 19.859297457790753 24.350395631124396 29.65337921650176 26.136927694583083 92-93 21.77543503316649 29.42206478548573 29.193264340579184 19.609235840768594 94-95 19.695174537160174 27.807968824302215 29.47898783760911 23.0178688009285 96-97 14.614920392802055 28.15534763102721 32.602630971799215 24.62710100437152 98-99 19.124936528278162 29.635853664434077 26.384388777393593 24.854821029894172 100-101 18.360876394238375 26.306536485698196 29.154102443462254 26.178484676601176 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8483.0 1 4961.0 2 1285.0 3 1036.5 4 778.0 5 711.5 6 1261.0 7 2257.5 8 2851.5 9 2696.0 10 1925.0 11 1191.0 12 987.5 13 896.5 14 790.5 15 683.5 16 604.5 17 580.0 18 580.0 19 618.5 20 663.0 21 781.5 22 882.0 23 939.0 24 1030.0 25 1215.5 26 1828.0 27 2489.5 28 2659.5 29 2882.0 30 3656.5 31 4496.0 32 5685.5 33 7488.5 34 9128.0 35 10073.0 36 11094.5 37 12095.0 38 11297.5 39 9784.5 40 8751.0 41 7711.5 42 6520.0 43 6338.0 44 6917.0 45 7611.5 46 13193.0 47 40875.0 48 100449.0 49 98896.0 50 42679.0 51 16352.0 52 4925.5 53 1407.0 54 654.5 55 447.0 56 321.0 57 222.0 58 180.5 59 158.0 60 134.0 61 103.0 62 91.5 63 71.0 64 42.0 65 33.5 66 22.5 67 15.5 68 15.5 69 13.0 70 9.5 71 5.5 72 3.5 73 4.5 74 3.0 75 1.0 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39513665562460204 2 0.0 3 0.0 4 0.0 5 8.059901185611465E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0012089851778417197 16-17 0.003223960474244586 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0033247092390647293 36-37 0.00886589130417261 38-39 0.014104827074820062 40-41 0.003022462944604299 42-43 9.067388833812897E-4 44-45 7.052413537410032E-4 46-47 4.0299505928057325E-4 48-49 4.0299505928057325E-4 50-51 7.052413537410032E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.0224629446042994E-4 60-61 0.0021157240612230096 62-63 0.0 64-65 5.037438241007166E-4 66-67 0.001611980237122293 68-69 0.0026194678853237257 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.12089851778417197 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14679095034294878 88-89 0.44168258497150825 90-91 0.34143756397546565 92-93 0.0 94-95 0.0 96-97 7.052413537410032E-4 98-99 0.0 100-101 1.0074876482014331E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 496284.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 12.962739076818918 #Duplication Level Percentage of deduplicated Percentage of total 1 69.66672884412112 9.030716283418364 2 10.472237766585836 2.7149777143732217 3 4.863831374743518 1.8914573107333705 4 2.749797923273021 1.425796519734668 5 1.9523720698874587 1.2654044861409999 6 1.3259342162531866 1.0312643566989868 7 1.0663433439035006 0.9675911373326563 8 0.8331778896971959 0.8640214070975489 9 0.6699620717527824 0.7816089174746718 >10 5.309954610458248 14.094147705749126 >50 0.6544177081390288 5.727567280025147 >100 0.32332276316607594 7.217037019126145 >500 0.031088727227507308 2.723037615558833 >1k 0.05595970900951316 18.013677652311983 >5k 0.020207672697879746 19.93072514930967 >10k+ 0.004663309084126096 12.320969444914605 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 31446 6.3362913170684525 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 17665 3.5594538610956628 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 12036 2.4252242667504897 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 9191 1.851963794923874 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 8983 1.8100523087586944 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 8954 1.8042088803991263 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 8262 1.6647725898880479 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 8206 1.6534887282281918 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 8153 1.6428093591572566 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 7899 1.5916289866286242 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 7732 1.557978899178696 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 7216 1.4540061738843082 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 6534 1.3165848586696327 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6262 1.2617775306074748 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5773 1.1632452386133747 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 5748 1.1582078003723675 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4948 0.9970097766601382 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 4945 0.9964052840712173 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 4876 0.9825019545260376 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 4645 0.9359560251791312 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 4399 0.8863876328876207 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 4327 0.8718798107535201 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3544 0.7141072450451758 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 3310 0.6669568231093487 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3036 0.6117464999879102 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2954 0.5952237025574066 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2890 0.5823278606604283 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2882 0.580715880423306 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2816 0.567417043467047 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2728 0.5496852608587018 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2698 0.5436403349694933 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2678 0.5396103843766875 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2652 0.53437144860604 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 2225 0.4483320034496377 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2209 0.4451080429753931 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2081 0.41931635918143645 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1777 0.3580611101707893 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1753 0.35322516945942245 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1636 0.3296499584915089 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1577 0.317761604242732 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1572 0.31675411659453057 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1566 0.31554513141668883 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1562 0.3147391412981277 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1487 0.2996268265751062 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1381 0.2782680884332358 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1362 0.2744396353700704 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1271 0.2561033601728043 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1263 0.254491379935682 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1157 0.23313264179381163 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1116 0.22487124307855985 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1054 0.21237839624086208 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1022 0.2059304752923729 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 988 0.1990795592846032 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 982 0.19787057410676148 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 928 0.18698970750618596 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 852 0.1716758952535242 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 771 0.15535459535266097 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 747 0.15051865464129408 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 703 0.14165276333712148 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 655 0.13198088191438773 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 641 0.1291599164994237 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 638 0.12855542391050284 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 625 0.12593595602517915 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT 608 0.12251049802129425 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 593 0.11948803507668997 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 588 0.11848054742848851 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 575 0.11586107954316481 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 565 0.11384610424676193 No Hit TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA 521 0.10498021294258933 No Hit TACCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 518 0.10437572035366846 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 511 0.10296523764618645 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 505 0.10175625246834474 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 3.022462944604299E-4 0.0 14-15 0.0 0.0 0.0 4.029950592805732E-4 0.0 16-17 0.0 0.0 0.0 5.037438241007166E-4 0.0 18-19 0.0 0.0 0.0 0.0018134777667625796 0.0 20-21 0.0 0.0 0.0 0.0030224629446042994 0.0 22-23 0.0 0.0 0.0 0.005339684535467595 0.0 24-25 0.0 0.0 0.0 0.008664393774532325 0.0 26-27 0.0 0.0 0.0 0.0158175560767625 0.0 28-29 0.0 0.0 0.0 0.043724963931942196 0.0 30-31 0.0 0.0 0.0 0.10498021294258933 0.0 32-33 0.0 0.0 0.0 0.22648322331568216 0.0 34-35 0.0 0.0 0.0 0.42173432953711987 0.0 36-37 0.0 1.007487648201433E-4 0.0 0.6635313651054637 0.0 38-39 0.0 2.014975296402866E-4 0.0 0.9705128515124404 0.0 40-41 0.0 2.014975296402866E-4 0.0 1.275479362623014 0.0 42-43 0.0 2.014975296402866E-4 0.0 1.6082525328239474 0.0 44-45 0.0 2.014975296402866E-4 0.0 1.9875716323717871 0.0 46-47 0.0 2.014975296402866E-4 0.0 2.4646170337951654 0.0 48-49 0.0 2.014975296402866E-4 0.0 2.8822206639746595 0.0 50-51 0.0 2.014975296402866E-4 0.0 3.3067759589267434 0.0 52-53 0.0 2.014975296402866E-4 0.0 3.6860950584745833 0.0 54-55 0.0 2.014975296402866E-4 0.0 4.115385545373214 0.0 56-57 0.0 2.014975296402866E-4 0.0 4.7239080848868795 0.0 58-59 0.0 2.014975296402866E-4 0.0 5.262611730380185 0.0 60-61 0.0 2.014975296402866E-4 0.0 5.9652336162358655 0.0 62-63 0.0 2.014975296402866E-4 0.0 6.583327288407444 0.0 64-65 0.0 2.014975296402866E-4 0.0 7.155781770115498 0.0 66-67 0.0 2.014975296402866E-4 0.0 7.822537095695207 0.0 68-69 0.0 2.014975296402866E-4 0.0 8.62016506677628 0.0 70-71 0.0 2.014975296402866E-4 0.0 9.424039461276205 0.0 72-73 0.0 2.014975296402866E-4 0.0 10.182173916547782 0.0 74-75 0.0 2.014975296402866E-4 0.0 11.106140838713317 0.0 76-77 0.0 2.014975296402866E-4 0.0 11.821960812760436 0.0 78-79 0.0 2.014975296402866E-4 0.0 12.98530680013863 0.0 80-81 0.0 2.014975296402866E-4 0.0 14.147040807279703 0.0 82-83 0.0 2.014975296402866E-4 0.0 15.035644912993366 0.0 84-85 0.0 2.014975296402866E-4 0.0 16.234454465588254 0.0 86-87 0.0 2.014975296402866E-4 0.0 17.42187940775846 0.0 88-89 0.0 2.014975296402866E-4 0.0 18.56870259770615 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGTTGC 15 6.6425884E-4 94.90307 6 CATTCCG 40 9.114774E-8 71.17731 9 GGTTGCA 20 0.0020817923 71.17731 7 TTGCAAG 20 0.0020817923 71.17731 9 CTTTAGG 35 3.4349068E-6 67.78791 2 GTAGGGT 30 1.3070845E-4 63.268715 3 GTGTAGG 50 4.1466774E-7 57.178047 1 AGGGTCT 25 0.005039921 56.941845 5 TGGGGCT 25 0.005039921 56.941845 6 GGCATTC 50 4.2670217E-7 56.941845 7 GTTGCAA 25 0.005039921 56.941845 8 TACTCCC 35 2.8004785E-4 54.230328 5 CCTTTAG 45 1.4869953E-5 52.94264 1 TTAGGCA 65 2.6046164E-6 43.80142 4 GCATTCC 70 4.3355394E-6 40.672745 8 GTAAGCG 140 0.0 40.672745 94-95 TGTAGGG 60 8.333913E-5 39.542946 2 CATAGTA 50 0.0016230498 37.96123 2 ACTCCCG 50 0.0016230498 37.96123 6 AGCAGAT 65 1.3350911E-4 36.501183 2 >>END_MODULE