##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938973_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1015343 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.82838410271209 33.0 31.0 34.0 30.0 34.0 2 31.820465596355124 33.0 31.0 34.0 30.0 34.0 3 31.852163259115393 33.0 31.0 34.0 30.0 34.0 4 35.31223931223242 37.0 35.0 37.0 33.0 37.0 5 35.40008745812991 37.0 35.0 37.0 33.0 37.0 6 35.54349121429901 37.0 35.0 37.0 33.0 37.0 7 35.527188349158855 37.0 35.0 37.0 33.0 37.0 8 35.556727135559115 37.0 35.0 37.0 33.0 37.0 9 37.04986984693842 39.0 37.0 39.0 33.0 39.0 10-11 36.97161156377697 39.0 37.0 39.0 33.0 39.0 12-13 36.86737240518721 39.0 37.0 39.0 32.5 39.0 14-15 37.754143673615715 40.0 37.0 41.0 32.0 41.0 16-17 37.704434856004326 40.0 37.0 41.0 32.5 41.0 18-19 37.79318466764433 40.0 37.0 41.0 33.0 41.0 20-21 37.908892364452214 40.0 37.0 41.0 33.0 41.0 22-23 37.91833104674972 40.0 37.0 41.0 33.0 41.0 24-25 37.82952066444541 40.0 37.0 41.0 33.0 41.0 26-27 37.42080902709725 39.5 36.5 41.0 32.0 41.0 28-29 37.18801232686885 39.0 36.0 41.0 31.5 41.0 30-31 37.12129152414504 39.0 35.5 41.0 32.0 41.0 32-33 36.959474778473876 39.0 35.0 41.0 31.5 41.0 34-35 36.70889492516322 39.0 35.0 41.0 31.0 41.0 36-37 36.272143994689486 38.5 35.0 41.0 30.0 41.0 38-39 35.774236391052085 38.0 35.0 40.0 29.5 41.0 40-41 35.539757500667264 38.0 35.0 40.0 30.0 41.0 42-43 35.612139936947415 38.0 35.0 40.0 30.0 41.0 44-45 35.70020278861429 38.0 35.0 40.0 29.5 41.0 46-47 35.447580768272395 38.0 35.0 40.0 27.0 41.0 48-49 35.27678873050782 38.0 35.0 40.0 27.0 41.0 50-51 34.132768926362814 37.5 33.5 39.5 24.5 40.5 52-53 34.04078769440475 37.5 33.5 39.5 24.0 40.5 54-55 34.62734465101941 38.0 34.5 40.0 25.0 41.0 56-57 34.81188721446841 38.0 35.0 40.0 26.0 41.0 58-59 34.756230653089645 38.0 35.0 40.0 26.0 41.0 60-61 34.38017251313103 38.0 34.5 40.0 24.5 41.0 62-63 33.79934071540356 37.0 33.5 39.5 22.5 41.0 64-65 33.1066802056054 36.0 32.5 39.0 22.0 41.0 66-67 32.67315774078317 36.0 32.0 39.0 20.0 40.0 68-69 32.37178618457014 35.0 32.0 38.0 20.0 40.0 70-71 31.721793521992076 35.0 31.5 37.0 16.5 39.0 72-73 31.082780892762347 35.0 31.0 37.0 12.5 39.0 74-75 30.633602634774654 35.0 31.0 36.0 7.5 37.5 76-77 29.94991396995892 34.5 29.5 35.5 4.5 37.0 78-79 29.540660643743053 34.0 29.0 35.0 2.0 36.5 80-81 29.312367347782967 34.0 29.0 35.0 2.0 36.0 82-83 28.90094972831841 34.0 29.0 35.0 2.0 36.0 84-85 28.553884746336955 34.0 29.0 35.0 2.0 35.5 86-87 27.92598314067266 34.0 27.0 35.0 2.0 35.0 88-89 27.173808752313256 33.5 25.5 35.0 2.0 35.0 90-91 26.513392518587317 33.0 25.0 35.0 2.0 35.0 92-93 26.167751685883488 33.0 24.0 35.0 2.0 35.0 94-95 25.698656020674786 32.5 22.0 35.0 2.0 35.0 96-97 24.833127327415465 32.0 16.0 35.0 2.0 35.0 98-99 23.873006461855745 32.0 2.0 34.5 2.0 35.0 100-101 20.826961923212153 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13387191025561407 1101 2 0.3362556576769329 1101 3 0.29238829258364873 1101 4 0.14184278746529344 1101 5 0.18757401081509073 1101 6 0.2418697044708864 1101 7 0.23741396340421517 1101 8 0.19684437722222725 1101 9 0.24759223135468034 1101 10-11 -0.00653936284486889 1101 12-13 -0.062242798882245154 1101 14-15 0.16916151161050408 1101 16-17 0.19172690583661023 1101 18-19 0.4065378297920148 1101 20-21 0.40770016887671545 1101 22-23 0.412308524206054 1101 24-25 0.31475418581073455 1101 26-27 0.41439529814723386 1101 28-29 0.2241897989220547 1101 30-31 0.2243931726704389 1101 32-33 0.24194173204247704 1101 34-35 0.19532809706073806 1101 36-37 0.16942060081684218 1101 38-39 0.49712562269283467 1101 40-41 0.2753669512656387 1101 42-43 0.509097784178131 1101 44-45 0.4985746059542677 1101 46-47 0.5196834259589309 1101 48-49 0.401529602395577 1101 50-51 0.4935273569805503 1101 52-53 0.5211468266591339 1101 54-55 0.5791569228465008 1101 56-57 0.49098040961947476 1101 58-59 0.44263693800566983 1101 60-61 0.41959070674992915 1101 62-63 0.48041469311764473 1101 64-65 0.32353401179886276 1101 66-67 0.40457015341228697 1101 68-69 0.3347877230076932 1101 70-71 0.21531938244723747 1101 72-73 0.25964672183713233 1101 74-75 0.21646766279666352 1101 76-77 0.15317049931662652 1101 78-79 0.19393472930718403 1101 80-81 0.2005319185990082 1101 82-83 0.5244561466044395 1101 84-85 0.5908604139443732 1101 86-87 0.7935243979750304 1101 88-89 0.3946843009039789 1101 90-91 0.26992705822979346 1101 92-93 0.3061108523222984 1101 94-95 0.23929478125174697 1101 96-97 -0.005352257493637325 1101 98-99 0.11317187571939158 1101 100-101 0.5780587863287003 1102 1 0.3007570807460951 1102 2 0.3684389708849132 1102 3 0.38989274891163817 1102 4 0.2815529823937055 1102 5 0.30623308734892873 1102 6 0.3242168955467264 1102 7 0.4064381713278564 1102 8 0.5007774460509324 1102 9 0.4212510203834867 1102 10-11 0.5999467506621201 1102 12-13 0.4899422393559192 1102 14-15 0.4629945286883057 1102 16-17 0.5450303386466757 1102 18-19 0.6229083662764054 1102 20-21 0.5972781114907093 1102 22-23 0.6099164823453265 1102 24-25 0.6903536338010667 1102 26-27 0.7696738905226042 1102 28-29 0.8399939504947085 1102 30-31 0.8400621968783568 1102 32-33 0.7808354126818884 1102 34-35 0.6608447436020484 1102 36-37 0.7042719607156371 1102 38-39 0.8318706057300389 1102 40-41 0.6772213587177731 1102 42-43 0.6380984166892034 1102 44-45 0.774185554145852 1102 46-47 1.0965478960871593 1102 48-49 0.97186483326211 1102 50-51 0.943777486357817 1102 52-53 1.0403205371990794 1102 54-55 1.0526288336049348 1102 56-57 0.9174136222006908 1102 58-59 0.9621556545832064 1102 60-61 1.2439998839105257 1102 62-63 1.1262976210688862 1102 64-65 1.0867908687938623 1102 66-67 0.956387185210346 1102 68-69 1.1996776085806857 1102 70-71 0.9378367765016371 1102 72-73 1.0078699407434648 1102 74-75 1.1769932219939498 1102 76-77 1.118307639216578 1102 78-79 1.1556131836801349 1102 80-81 1.1035133457739832 1102 82-83 1.1871782442411494 1102 84-85 1.1272355505092655 1102 86-87 1.044053407232692 1102 88-89 1.0268446137094536 1102 90-91 1.2003939664006857 1102 92-93 1.4453552890999433 1102 94-95 1.5187324214104514 1102 96-97 1.6964992749081809 1102 98-99 1.5163574314666164 1102 100-101 1.3872445720799504 1103 1 0.17362220765810576 1103 2 0.2898735734340683 1103 3 0.30187629831225493 1103 4 0.3121175527194424 1103 5 0.10528533982886756 1103 6 0.20985995659889767 1103 7 0.22259927691841597 1103 8 -0.04780064547276197 1103 9 0.2837621492369422 1103 10-11 0.15996214437941347 1103 12-13 0.25908760084980287 1103 14-15 0.380275125137409 1103 16-17 0.38836250404872175 1103 18-19 0.46858003207191246 1103 20-21 0.32354450321486183 1103 22-23 0.3490998111792507 1103 24-25 0.4595845797679772 1103 26-27 0.2684630712666731 1103 28-29 0.32521598189099876 1103 30-31 0.2116435566950159 1103 32-33 0.30614486660699924 1103 34-35 0.2168658123166125 1103 36-37 0.08189880092540847 1103 38-39 0.46833648394735405 1103 40-41 0.43986402343916353 1103 42-43 0.456813271051999 1103 44-45 0.34201096182088975 1103 46-47 0.1936615740942429 1103 48-49 0.28525966487897136 1103 50-51 -0.05019254184485078 1103 52-53 0.1633439092273079 1103 54-55 0.31894129160249207 1103 56-57 0.19091919667928892 1103 58-59 0.0922072924632431 1103 60-61 0.20118524609367938 1103 62-63 0.3366768923946353 1103 64-65 0.15439226341869272 1103 66-67 0.3153003198421871 1103 68-69 0.1400878396587686 1103 70-71 0.030583348814765543 1103 72-73 -0.048497486610255436 1103 74-75 -0.029377364518168747 1103 76-77 0.12243005373262505 1103 78-79 -0.016877439363337743 1103 80-81 -0.1367854531714343 1103 82-83 0.026098501339205882 1103 84-85 0.01170053267437865 1103 86-87 0.03707855987748232 1103 88-89 0.26999469670915843 1103 90-91 0.43608958436078993 1103 92-93 0.18424050825937854 1103 94-95 0.3193751665467808 1103 96-97 0.1666630341917994 1103 98-99 0.10135200345809281 1103 100-101 0.28753004512084246 1104 1 0.1306576534153585 1104 2 0.13734940587230682 1104 3 0.058052452984654224 1104 4 0.2498189490674534 1104 5 0.3372939327397191 1104 6 0.3247901391982566 1104 7 0.2720085142975819 1104 8 0.34317679813626256 1104 9 0.25905753054735925 1104 10-11 0.3747849155931604 1104 12-13 0.36649898645667633 1104 14-15 0.4608336643425659 1104 16-17 0.5741842011486185 1104 18-19 0.5878066700955458 1104 20-21 0.6387969199710355 1104 22-23 0.5429773621996645 1104 24-25 0.4998638493705556 1104 26-27 0.539139762995994 1104 28-29 0.7296198487008851 1104 30-31 0.7701827618507551 1104 32-33 0.7067893349206358 1104 34-35 0.6739012580738688 1104 36-37 0.7784616365859875 1104 38-39 0.94040952368956 1104 40-41 0.675094957918212 1104 42-43 0.6490796512883037 1104 44-45 0.6379293291678252 1104 46-47 0.7140697373595515 1104 48-49 0.9404691170809016 1104 50-51 0.8880459114311918 1104 52-53 0.8948154452101278 1104 54-55 1.0686727631384727 1104 56-57 0.892315544692174 1104 58-59 0.9514983773182379 1104 60-61 0.9847513040958304 1104 62-63 0.9081054638232118 1104 64-65 0.678559825180244 1104 66-67 1.0693282468024492 1104 68-69 1.0063606645782102 1104 70-71 0.9161902231434418 1104 72-73 0.8306647045820164 1104 74-75 0.8315248911209316 1104 76-77 0.7577683995972855 1104 78-79 0.7967638066087339 1104 80-81 1.0882413996749705 1104 82-83 1.0089126796421048 1104 84-85 0.9784030031362505 1104 86-87 0.8002364546143248 1104 88-89 0.7554941596522333 1104 90-91 0.7083774468742128 1104 92-93 0.8185047402679686 1104 94-95 0.6684621697691249 1104 96-97 0.618864967596739 1104 98-99 0.35806521505852373 1104 100-101 0.4110531385687466 1105 1 0.1382867587810246 1105 2 0.13171365080592068 1105 3 0.12157224832362346 1105 4 0.2018816042234235 1105 5 0.18470514007043448 1105 6 0.22917617839226523 1105 7 0.11091594885908762 1105 8 0.2113738913434986 1105 9 0.15518826936833108 1105 10-11 0.3611356471939544 1105 12-13 0.17779088692510214 1105 14-15 0.2247531325447838 1105 16-17 0.384987513519647 1105 18-19 0.49096093245564987 1105 20-21 0.41717792732047343 1105 22-23 0.3953908871204419 1105 24-25 0.5045480554399688 1105 26-27 0.3606207030461803 1105 28-29 0.47614290649355695 1105 30-31 0.44267497341108 1105 32-33 0.33559943069951004 1105 34-35 0.43625635298363363 1105 36-37 0.27687386454771 1105 38-39 0.2950598572888268 1105 40-41 0.47867641452356935 1105 42-43 0.5640625367770653 1105 44-45 0.5381331211017226 1105 46-47 0.40101568848513836 1105 48-49 0.4602343719489568 1105 50-51 0.3312766542633767 1105 52-53 0.45746057314555344 1105 54-55 0.27379842554582723 1105 56-57 0.176003610440425 1105 58-59 0.22531328655338712 1105 60-61 0.32114003080292974 1105 62-63 0.26270064323588116 1105 64-65 0.6098276806517617 1105 66-67 0.5241268838812161 1105 68-69 0.3700262006769037 1105 70-71 0.43509696688483857 1105 72-73 0.5521811884413346 1105 74-75 0.5510335397430772 1105 76-77 0.40311026641608194 1105 78-79 0.6337185601196573 1105 80-81 0.5802579704439168 1105 82-83 0.7817041723038116 1105 84-85 0.6912474661016041 1105 86-87 0.6290673864192406 1105 88-89 0.5794096834944433 1105 90-91 0.589126852991761 1105 92-93 0.7032230193682096 1105 94-95 0.7604197979831078 1105 96-97 0.8058220609822477 1105 98-99 1.0258832485833551 1105 100-101 1.0828339168333159 1106 1 0.14550556815674298 1106 2 0.11346746576242595 1106 3 0.2571384636993237 1106 4 0.2559449269632026 1106 5 0.17538395097980697 1106 6 0.2231352980766772 1106 7 0.16157328865647713 1106 8 0.17873481903185962 1106 9 0.15792541013191652 1106 10-11 0.2517437397583038 1106 12-13 0.3710000087395784 1106 14-15 0.4520753355490328 1106 16-17 0.35443804049911876 1106 18-19 0.36084663286547425 1106 20-21 0.392964349398234 1106 22-23 0.44179341082812584 1106 24-25 0.27762098053815976 1106 26-27 0.32561938240238675 1106 28-29 0.3457879089218352 1106 30-31 0.31805306094845776 1106 32-33 0.37799635658156916 1106 34-35 0.3634707744138481 1106 36-37 0.27492058977711764 1106 38-39 0.49577215290855037 1106 40-41 0.6788146561076616 1106 42-43 0.5339614436248183 1106 44-45 0.4693414249690804 1106 46-47 0.602168908725929 1106 48-49 0.5890227025699986 1106 50-51 0.5233610987835533 1106 52-53 0.5673083156966925 1106 54-55 0.6767204564403713 1106 56-57 0.5405620041967651 1106 58-59 0.5966410447818475 1106 60-61 0.850020593482391 1106 62-63 0.9310561009028575 1106 64-65 0.9203280768449034 1106 66-67 0.9622195760348831 1106 68-69 0.8582106988748563 1106 70-71 0.8338749453626058 1106 72-73 0.7023373226187424 1106 74-75 0.8080531875145986 1106 76-77 0.7827889665617782 1106 78-79 0.7609679147082566 1106 80-81 0.8161696832763141 1106 82-83 0.7711475422459593 1106 84-85 0.911491444391924 1106 86-87 1.0224285952769243 1106 88-89 0.9765272719136711 1106 90-91 1.0673415555912982 1106 92-93 0.9690505092077721 1106 94-95 0.7869466326129455 1106 96-97 0.8143955952806472 1106 98-99 0.6833061187257279 1106 100-101 0.9084290585682169 1107 1 -0.0262718907548809 1107 2 0.23284848058145613 1107 3 0.021303955234252214 1107 4 0.08130385755209346 1107 5 0.05975304955067884 1107 6 0.13958961637656842 1107 7 -0.0048583516624987055 1107 8 0.08895475421406474 1107 9 0.03925565925984387 1107 10-11 0.00887390312222891 1107 12-13 0.13250392996864235 1107 14-15 0.09997184973720152 1107 16-17 0.17161743569798915 1107 18-19 0.17435425679033756 1107 20-21 0.21489993568588517 1107 22-23 0.26328986893983597 1107 24-25 0.22865818891994394 1107 26-27 0.28693383940905903 1107 28-29 0.3498472675060711 1107 30-31 0.3580147557770559 1107 32-33 0.2806222606083537 1107 34-35 0.38700124125039537 1107 36-37 0.4326088376350796 1107 38-39 0.5207426715333554 1107 40-41 0.36190018095860665 1107 42-43 0.2584208830251242 1107 44-45 -0.07041101394468541 1107 46-47 0.18109034395348544 1107 48-49 0.23604508367553478 1107 50-51 0.24562283566950072 1107 52-53 0.4136588100766829 1107 54-55 0.44240021861019585 1107 56-57 0.4673244956409732 1107 58-59 0.3718538054755456 1107 60-61 0.5037855506416307 1107 62-63 0.5204619790355025 1107 64-65 0.4549759084176017 1107 66-67 0.30740730848418707 1107 68-69 0.4120678111020055 1107 70-71 0.06894355656305251 1107 72-73 0.2975693802125683 1107 74-75 0.2669761483517874 1107 76-77 0.3104220863097602 1107 78-79 0.3961791910250234 1107 80-81 0.2534403264238563 1107 82-83 0.37828760145610474 1107 84-85 0.3613844455546875 1107 86-87 0.47668607694899023 1107 88-89 0.7017350846842625 1107 90-91 0.7232629825723258 1107 92-93 0.6811659112677582 1107 94-95 0.7042740531781639 1107 96-97 0.8507236590758716 1107 98-99 0.7840716036634277 1107 100-101 0.9441942961097602 1108 1 0.11268084325809724 1108 2 0.11761215064633035 1108 3 0.19956777976313944 1108 4 0.11072616215511744 1108 5 0.07220593802766473 1108 6 0.08899956385880614 1108 7 0.14955622974003546 1108 8 0.1030974789591994 1108 9 0.21614475410074618 1108 10-11 0.2796971962949186 1108 12-13 0.2050773003733397 1108 14-15 0.20619211541529836 1108 16-17 0.24818679003842448 1108 18-19 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2316 84-85 -1.3567836252494807 2316 86-87 -1.4838450368240963 2316 88-89 -1.448937861826515 2316 90-91 -1.8022763151279264 2316 92-93 -2.147373389732465 2316 94-95 -1.9630563200815168 2316 96-97 -2.0098963064064392 2316 98-99 -2.262123447700617 2316 100-101 -2.3273710119589985 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3403.0 3 761.0 4 651.0 5 700.0 6 841.0 7 1065.0 8 1286.0 9 1617.0 10 2185.0 11 3077.0 12 4462.0 13 6683.0 14 5965.0 15 5978.0 16 7212.0 17 8311.0 18 7656.0 19 6673.0 20 7070.0 21 7748.0 22 8749.0 23 9726.0 24 11137.0 25 12868.0 26 14791.0 27 16962.0 28 20329.0 29 23793.0 30 28297.0 31 34310.0 32 42426.0 33 54187.0 34 75866.0 35 134124.0 36 143511.0 37 179052.0 38 110118.0 39 11747.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.77108481344165 16.150444007796438 13.642088916154693 17.43638226260722 2 11.850528861934064 24.69835994152998 40.11780743334151 23.333303763194444 3 26.578717271577517 30.078160810766853 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30.22539145588273 24.71194011902861 28.241351303932994 24-25 20.38496930171947 29.895792928429167 29.098924019969036 20.620313749882328 26-27 25.7006985176522 24.11780911981256 31.50983410144154 18.671658261093704 28-29 18.896489524757648 26.05917982129744 36.26986747623295 18.774463177711954 30-31 17.389009572320695 35.36761575096196 31.47442518766164 15.7689494890557 32-33 16.7645895002215 33.77674973722712 28.056209989656626 21.40245077289475 34-35 19.232330896147428 27.639634352960236 28.762794732042916 24.365240018849416 36-37 23.37166627824067 27.770528808591095 31.831435983685118 17.026368929483116 38-39 27.381432916031017 22.554210716893326 33.50384555263371 16.560510814441944 40-41 18.811810673129234 26.950591028240762 37.918640882338394 16.318957416291607 42-43 16.301609030717678 33.45342231329767 32.341947845893614 17.903020810091036 44-45 17.20956122469175 35.183416787064345 27.245828962732798 20.361193025511113 46-47 17.105873144589083 30.326744287874973 24.273012389278954 28.29437017825699 48-49 20.59601446317082 28.754335413144027 27.759509950481405 22.890140173203744 50-51 25.83204020315339 30.3715209190197 28.316532537398516 15.479906340428396 52-53 18.24894702673602 30.048927629860106 34.36720661235542 17.33491873104846 54-55 14.204089751081217 29.261130443258406 35.99785463467684 20.53692517098354 56-57 19.37250653587497 35.116285396987934 26.51336731740979 18.99784074972731 58-59 16.987992864070637 30.390660973618132 29.091079049657225 23.53026711265401 60-61 17.826422064249417 25.70144721613715 32.19950848143902 24.27262223817441 62-63 27.571615581824965 24.547885050943016 28.798314729591873 19.082184637640147 64-65 23.896084230778555 26.545294035269684 33.44246346425155 16.116158269700207 66-67 17.184838391986656 27.918767909018477 35.53748088467458 19.358912814320288 68-69 15.744024359400141 34.63918974901642 32.28531224587473 17.33147364570872 70-71 17.148253424132083 32.694033326420644 26.810658475707505 23.347054773739767 72-73 16.656909201133285 29.842057728234376 25.47673425959147 28.024298811040865 74-75 19.194779074727883 29.90144696872968 29.18706863871708 21.71670531782536 76-77 23.620755767208856 24.735135135135135 31.142457789597376 20.50165130805863 78-79 18.54910956102083 25.461444815835126 35.67355632933653 20.315889293807516 80-81 17.091967226577303 33.57658130972982 31.48000835067285 17.851443113020025 82-83 16.670138158789843 31.933357246224443 28.278395527464777 23.118109067520933 84-85 18.43075362713206 26.70860493017848 28.747022226619993 26.113619216069463 86-87 21.702648093139416 26.791039902475127 31.13107477236611 20.375237232019348 88-89 24.983226723243902 22.159428263812472 32.74074352620787 20.116601486735753 90-91 18.696493856876202 25.0409244673881 36.40857365117508 19.854008024560617 92-93 16.42357421862435 30.656421367061597 32.86451866408604 20.055485750228016 94-95 16.418494735409475 32.401123442584414 28.927062841022977 22.253318980983135 96-97 16.100672877974866 27.954054068766474 26.318055468265566 29.627217584993094 98-99 19.363117720185922 26.99524312896406 28.114038213332698 25.52760093751732 100-101 23.50663429467975 26.97605102991506 29.304152365383217 20.213162310021975 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 22882.0 1 13531.5 2 4778.5 3 4837.5 4 3229.0 5 3085.0 6 6739.5 7 12581.0 8 15934.5 9 14374.5 10 9333.0 11 5435.5 12 4714.0 13 4281.0 14 3533.0 15 3055.5 16 2727.0 17 2251.0 18 1868.0 19 1653.0 20 1608.5 21 1726.5 22 1946.0 23 2231.0 24 2554.0 25 2988.0 26 3723.5 27 4469.5 28 5002.5 29 5707.0 30 6552.0 31 7341.0 32 8259.0 33 9358.0 34 10214.0 35 10807.0 36 11732.0 37 12488.0 38 12728.5 39 12736.5 40 12520.5 41 12493.5 42 12632.5 43 12617.5 44 15110.0 45 18457.5 46 30031.5 47 59605.5 48 202960.0 49 205959.0 50 67615.5 51 36952.5 52 17760.5 53 10612.0 54 7756.0 55 5943.0 56 4704.0 57 3752.0 58 3047.5 59 2634.5 60 2296.5 61 1867.5 62 1278.5 63 814.0 64 537.5 65 388.5 66 287.5 67 231.0 68 185.5 69 142.0 70 120.5 71 94.0 72 80.0 73 64.0 74 44.5 75 39.5 76 32.5 77 27.0 78 23.5 79 15.0 80 9.5 81 10.0 82 8.5 83 6.5 84 5.5 85 2.5 86 2.5 87 2.0 88 0.5 89 0.5 90 0.5 91 0.0 92 0.5 93 0.5 94 0.5 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3039366992238091 2 0.0793820413397246 3 0.08824604099304373 4 0.09277652970474018 5 0.10420124036901815 6 0.054365864540357293 7 0.05692657555131616 8 0.058108442171758715 9 0.060472175412643804 10-11 0.05756675330405587 12-13 0.05628639779857644 14-15 0.054415108982875736 16-17 0.05658186445368708 18-19 0.06258968644093671 20-21 0.06524888633693245 22-23 0.07849564137439269 24-25 0.08593155219467706 26-27 0.0824844412183863 28-29 0.07957901910979837 30-31 0.07396515266269625 32-33 0.0673171529227069 34-35 0.3063004324646942 36-37 0.47811429241152986 38-39 0.4496017601933534 40-41 0.43266167196701016 42-43 0.4632424707709612 44-45 0.4730421148321306 46-47 0.46796993725273134 48-49 0.4840236255137426 50-51 0.4801333145547859 52-53 0.4797393590146384 54-55 0.47200798153924334 56-57 0.45063589348624067 58-59 0.4335973163748605 60-61 0.3927244290845557 62-63 0.46329171521347956 64-65 0.48540246990425895 66-67 0.49520211396542846 68-69 0.5059866468769667 70-71 0.5046078024864503 72-73 0.49298611405209863 74-75 0.4948081584252809 76-77 0.47082611491880083 78-79 0.4508328712563144 80-81 0.4575793598813406 82-83 0.46590167066695687 84-85 0.46590167066695687 86-87 0.46575393733940157 88-89 0.47343607037227814 90-91 0.48702753650736746 92-93 0.49180424743165607 94-95 0.4979105583039426 96-97 0.49062238081121357 98-99 0.49392175845994907 100-101 0.4725989148494647 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1015343.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.890990903218945 #Duplication Level Percentage of deduplicated Percentage of total 1 75.59599100255654 17.304671423593433 2 12.50592893083858 5.7254621078425725 3 4.639373916072902 3.1859959832836813 4 2.286695449740659 2.093788989537823 5 1.320030238107133 1.5108400086242157 6 0.8311928415623858 1.141609666501517 7 0.520462364793167 0.8339729480563743 8 0.3725308254148593 0.6822079788592152 9 0.2874035065729515 0.5921055949063194 >10 1.3895499345374767 5.6765236030056165 >50 0.10990455739059983 1.8163264295377 >100 0.09223362855148169 4.371515902443796 >500 0.01637793404185189 2.511680512862266 >1k 0.021549913212963012 10.439486609611711 >5k 0.004740980906851863 8.416566614493167 >10k+ 0.006033975699629643 33.69724562684056 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 64108 6.31392544194425 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 52011 5.122505399653122 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 40722 4.0106643764717935 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 24136 2.3771277292501156 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 23254 2.290260532647588 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 22239 2.190294314335156 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 18085 1.7811714858919598 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 17466 1.7202068660541314 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15260 1.502940385662776 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 15087 1.4859018085513958 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13360 1.3158115040927056 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 12773 1.2579985285760575 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 12634 1.2443085735559312 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 10415 1.0257617376590966 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 9962 0.9811462727373902 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9610 0.9464781852044089 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 9298 0.9157496530729025 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 9277 0.9136813864871279 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 8755 0.8622701884978771 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8074 0.7951992577877623 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 6736 0.663421129608418 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 6420 0.6322986419367642 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 6302 0.6206769535024125 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5795 0.5707430887887148 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 5080 0.5003235359873461 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 4659 0.45885971538682 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 4599 0.4529503822846072 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4419 0.435222382977969 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 4110 0.4047893175015734 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 4058 0.3996678954796556 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 3969 0.39090238471137345 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3687 0.3631285191309735 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3685 0.3629315413608997 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3580 0.3525902084320274 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 3419 0.33673349794108987 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 2826 0.2783295891142205 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 2762 0.2720263004718602 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 2691 0.26503358963424184 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2626 0.25863181210684466 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2486 0.24484336820168162 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 2443 0.2406083461450958 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2311 0.22760781332022773 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 2144 0.2111601695190689 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2097 0.2065311919223356 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 2039 0.20081883659019661 No Hit TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA 1884 0.18555305940948036 No Hit GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1838 0.1810225706977839 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 1782 0.17550719313571866 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1747 0.1720600821594279 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1725 0.16989332668861656 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1612 0.1587640826794492 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1542 0.15186986072686767 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1501 0.14783181644035562 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1462 0.14399074992391733 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1458 0.14359679438376982 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1413 0.13916479455711026 No Hit TTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCG 1394 0.13729350574140955 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1387 0.1366040835461514 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1371 0.13502826138556132 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1362 0.1341418614202294 No Hit GTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 1338 0.1317781281793443 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1327 0.13069475044393863 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1239 0.1220277285606933 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 1231 0.12123981748039825 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 1230 0.12114132859536138 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1229 0.12104283971032449 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1216 0.11976248420484507 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 1194 0.1175957287340337 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 1182 0.11641386211359117 No Hit CATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1166 0.11483803995300111 No Hit CAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTA 1154 0.11365617333255855 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 1144 0.11267128448218976 No Hit CTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTA 1053 0.10370879594383375 No Hit ACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGAT 1016 0.10006470719746924 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.4773332755531874E-4 0.0 12-13 0.0 0.0 0.0 2.954666551106375E-4 0.0 14-15 0.0 0.0 0.0 3.4471109762907706E-4 0.0 16-17 0.0 0.0 0.0 5.90933310221275E-4 0.0 18-19 0.0 0.0 0.0 9.848888503687916E-4 0.0 20-21 0.0 0.0 0.0 0.0014280888330347478 0.0 22-23 0.0 0.0 0.0 0.003151644321180133 0.0 24-25 0.0 0.0 0.0 0.006254044199841827 0.0 26-27 0.0 0.0 0.0 0.009553421848577277 0.0 28-29 0.0 0.0 0.0 0.020731910300263064 0.0 30-31 0.0 0.0 0.0 0.04569884265711193 0.0 32-33 0.0 0.0 0.0 0.08967412982607847 0.0 34-35 0.0 0.0 0.0 0.19362914798250444 0.0 36-37 0.0 0.0 0.0 0.3469270975424068 0.0 38-39 0.0 0.0 0.0 0.4960885139307603 0.0 40-41 0.0 0.0 0.0 0.6574133076211685 0.0 42-43 0.0 0.0 0.0 0.8103665460834417 0.0 44-45 0.0 0.0 0.0 0.9659789844417108 0.0 46-47 0.0 0.0 0.0 1.2081631527473968 0.0 48-49 0.0 0.0 0.0 1.5067814521792142 0.0 50-51 0.0 0.0 0.0 1.7635912199128767 0.0 52-53 0.0 0.0 0.0 1.9711073006855813 0.0 54-55 0.0 0.0 0.0 2.181479559124355 0.0 56-57 0.0 0.0 0.0 2.40317803934237 0.0 58-59 0.0 0.0 0.0 2.8128918010957875 0.0 60-61 0.0 0.0 0.0 3.5112764848922975 0.0 62-63 0.0 0.0 0.0 4.0410481975056705 0.0 64-65 0.0 0.0 0.0 4.372069340114622 0.0 66-67 0.0 0.0 0.0 4.766566569129841 0.0 68-69 0.0 0.0 0.0 5.192580241356861 0.0 70-71 0.0 0.0 0.0 5.7173782652758725 0.0 72-73 0.0 0.0 0.0 6.393258238841455 0.0 74-75 0.0 0.0 0.0 7.044663724475374 0.0 76-77 0.0 0.0 0.0 7.531789749867778 0.0 78-79 0.0 0.0 0.0 8.06732306225581 0.0 80-81 0.0 0.0 0.0 8.663033083401373 0.0 82-83 0.0 0.0 0.0 9.279671992617274 0.0 84-85 0.0 0.0 0.0 10.488820034215038 0.0 86-87 0.0 0.0 0.0 11.842894470144572 0.0 88-89 0.0 0.0 0.0 12.626521283940502 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCCGGA 25 0.0016065364 38.045967 94-95 TATCACG 50 0.0016368495 37.89966 2 ATCACGC 50 0.0016368495 37.89966 3 AGTACTC 400 0.0 34.346565 5 GTATTAG 60 0.0040109 31.569042 6 GGACTAT 60 0.0040109 31.569042 6 CCGTATC 75 3.3003744E-8 28.53448 94-95 CCTAGTA 100 5.0426075E-5 28.424744 2 ACCACTC 720 0.0 28.402374 94-95 TAATGCG 35 0.008333634 27.182419 34-35 TCACGCA 70 0.008500027 27.071188 4 CGTATCT 45 8.886956E-4 26.420813 94-95 TGGGGTC 115 1.3087556E-4 24.706207 6 GGGTCGC 50 0.00164248 23.77873 90-91 CGTCAAG 70 1.7637674E-5 23.77873 68-69 AAGTACT 400 0.0 23.687286 4 AAAGTAC 385 0.0 23.379658 3 TCCTAGT 145 2.3443143E-5 22.91794 1 CATGGGG 1285 0.0 22.857773 4 CGCCGTA 115 3.5797711E-9 22.744873 94-95 >>END_MODULE