##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938972_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1026241 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.82900507775464 33.0 31.0 34.0 30.0 34.0 2 31.805672351815996 33.0 31.0 34.0 30.0 34.0 3 31.838979343058796 33.0 31.0 34.0 30.0 34.0 4 35.30589695792704 37.0 35.0 37.0 33.0 37.0 5 35.38931011331646 37.0 35.0 37.0 33.0 37.0 6 35.53272769261801 37.0 35.0 37.0 33.0 37.0 7 35.502561289209844 37.0 35.0 37.0 33.0 37.0 8 35.52830572935597 37.0 35.0 37.0 33.0 37.0 9 37.023668904282715 39.0 37.0 39.0 33.0 39.0 10-11 36.94085404890274 39.0 37.0 39.0 33.0 39.0 12-13 36.824569959687835 39.0 37.0 39.0 32.5 39.0 14-15 37.71378263000601 40.0 37.0 41.0 32.0 41.0 16-17 37.66728575451575 40.0 37.0 41.0 32.5 41.0 18-19 37.78803906684687 40.0 37.0 41.0 33.0 41.0 20-21 37.657357287420794 40.0 37.0 41.0 32.0 41.0 22-23 37.8278557375899 40.0 37.0 41.0 33.0 41.0 24-25 37.765445933265184 40.0 37.0 41.0 33.0 41.0 26-27 37.3992853530506 39.5 36.5 41.0 32.0 41.0 28-29 37.16152346281234 39.0 36.0 41.0 31.5 41.0 30-31 37.105376319987215 39.0 35.5 41.0 32.0 41.0 32-33 36.95675820786735 39.0 35.0 41.0 31.5 41.0 34-35 36.70355598733631 39.0 35.0 41.0 31.0 41.0 36-37 36.259938455002285 38.5 35.0 41.0 30.0 41.0 38-39 35.81535185205035 38.0 35.0 40.0 30.0 41.0 40-41 35.56876649831764 38.0 35.0 40.0 30.0 41.0 42-43 35.64186385069394 38.0 35.0 40.0 30.0 41.0 44-45 35.71539920934751 38.0 35.0 40.0 29.5 41.0 46-47 35.43603597985269 38.0 35.0 40.5 27.0 41.0 48-49 35.31824590909932 38.0 35.0 40.5 27.0 41.0 50-51 34.12803815088269 37.5 33.5 39.0 24.5 40.5 52-53 34.085810253147166 37.5 33.5 39.5 24.0 40.5 54-55 34.66692618985209 38.0 34.5 40.0 25.0 41.0 56-57 34.839887024587796 38.0 35.0 40.0 26.0 41.0 58-59 34.794386016539974 38.0 35.0 40.0 26.0 41.0 60-61 34.462845471970034 38.0 34.5 40.0 24.5 41.0 62-63 33.896152073440845 37.5 33.5 40.0 22.5 41.0 64-65 33.17555622899494 36.0 32.5 39.0 22.0 41.0 66-67 32.76917117908951 36.0 32.0 39.0 21.0 40.0 68-69 32.51606250383682 36.0 33.0 38.5 21.0 40.0 70-71 31.908349500750795 35.0 31.5 37.5 18.5 39.0 72-73 31.26667322782855 35.0 31.0 37.0 15.0 39.0 74-75 30.814139661151717 35.0 31.0 36.0 10.0 37.5 76-77 30.160225522075223 34.5 30.0 35.5 7.0 37.0 78-79 29.707672954013724 34.0 29.5 35.0 2.0 36.5 80-81 29.474040698042664 34.0 29.0 35.0 2.0 36.0 82-83 29.16378219151252 34.0 29.0 35.0 2.0 36.0 84-85 28.87810368129903 34.0 29.0 35.0 2.0 35.5 86-87 28.303835064083387 34.0 29.0 35.0 2.0 35.0 88-89 27.609743715170218 33.5 27.0 35.0 2.0 35.0 90-91 26.977461434497357 33.0 25.0 35.0 2.0 35.0 92-93 26.697857520796774 33.0 25.0 35.0 2.0 35.0 94-95 26.297701514556522 33.0 24.5 35.0 2.0 35.0 96-97 25.501227294563364 33.0 20.5 35.0 2.0 35.0 98-99 24.692933238878588 32.0 10.0 35.0 2.0 35.0 100-101 22.13332345910951 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.31183258758649757 1101 2 0.3068941814229156 1101 3 0.2145909713390033 1101 4 0.16983593118273177 1101 5 0.0026328836994764515 1101 6 0.19259564771670057 1101 7 0.14613029110540765 1101 8 0.12799494832016478 1101 9 0.12680344582619085 1101 10-11 0.23049673986598407 1101 12-13 0.19958353726294575 1101 14-15 0.037978140954166406 1101 16-17 0.23181485907191046 1101 18-19 0.29123184602467944 1101 20-21 0.3783081492692517 1101 22-23 0.4334893584296893 1101 24-25 0.5056521182557603 1101 26-27 0.3393789954365687 1101 28-29 0.32939881935539717 1101 30-31 0.3775341919851698 1101 32-33 0.5092163023444485 1101 34-35 0.2740391043122443 1101 36-37 0.4352414721256608 1101 38-39 0.4282283710495278 1101 40-41 0.4619188665496168 1101 42-43 0.5568062662522593 1101 44-45 0.6933815008525386 1101 46-47 0.5954900009215081 1101 48-49 0.6600357552235678 1101 50-51 0.39914443343014483 1101 52-53 0.40684374007857826 1101 54-55 0.6022751439718732 1101 56-57 0.38702212280266934 1101 58-59 0.489456400834257 1101 60-61 0.526180294042021 1101 62-63 0.40375351158683515 1101 64-65 0.40955251861178965 1101 66-67 0.2626429964982222 1101 68-69 0.2735532121092916 1101 70-71 0.15674930811421106 1101 72-73 0.29759546791856195 1101 74-75 0.3928505676728449 1101 76-77 0.23631330449600796 1101 78-79 0.20383390763266718 1101 80-81 0.23156037714278455 1101 82-83 0.4771888076419728 1101 84-85 0.17776402711668382 1101 86-87 0.20161877439691267 1101 88-89 0.2844431479003049 1101 90-91 0.3031276799748319 1101 92-93 0.5397566947252272 1101 94-95 0.639244222243363 1101 96-97 0.6951566442652535 1101 98-99 0.7454528800231017 1101 100-101 0.8606422234192301 1102 1 0.12291399955632087 1102 2 0.05371007024983854 1102 3 0.18479781837987375 1102 4 0.2551611367798472 1102 5 0.20782296888686602 1102 6 0.17110329791636758 1102 7 0.27139229522055075 1102 8 0.0494001236626076 1102 9 0.44621450544454433 1102 10-11 0.10536133101136613 1102 12-13 0.2023510058064275 1102 14-15 0.3540784941389177 1102 16-17 0.38997400373663993 1102 18-19 0.37493374783803546 1102 20-21 0.486718561489063 1102 22-23 0.5632847548491284 1102 24-25 0.5413119199338681 1102 26-27 0.6264601894702437 1102 28-29 0.44174329948155133 1102 30-31 0.2896097773680637 1102 32-33 0.3317105174217829 1102 34-35 0.31861345579196865 1102 36-37 0.2366583775606088 1102 38-39 0.1739209272482043 1102 40-41 0.12776186278700408 1102 42-43 -0.049051408625473414 1102 44-45 0.29626659081529283 1102 46-47 0.2893762510737403 1102 48-49 0.36513930694173524 1102 50-51 0.2772043530501733 1102 52-53 0.35316734961392626 1102 54-55 0.30667681310634265 1102 56-57 0.38840665831023813 1102 58-59 0.5210379121573183 1102 60-61 0.41135679026979943 1102 62-63 0.38472255824439827 1102 64-65 0.5150896991590699 1102 66-67 0.5030938679223098 1102 68-69 0.5282756960685546 1102 70-71 0.5494526136927398 1102 72-73 0.6525862056324137 1102 74-75 0.8046697575913768 1102 76-77 0.6559878104539969 1102 78-79 0.5899141978723286 1102 80-81 0.5155375835860028 1102 82-83 0.40743962650498844 1102 84-85 0.42867102607187135 1102 86-87 0.3643914349485513 1102 88-89 0.3205756787158336 1102 90-91 0.30737422985336593 1102 92-93 0.2898031664031464 1102 94-95 0.45306425826692376 1102 96-97 0.41758932443114105 1102 98-99 0.2911890234507233 1102 100-101 0.9132177158587673 1103 1 -0.03413674445094372 1103 2 0.20293334990790513 1103 3 0.21200562819638336 1103 4 0.25125278504540915 1103 5 0.2155557374892041 1103 6 0.3238044940958247 1103 7 0.1593048135678501 1103 8 0.12575717828053712 1103 9 0.1614041336776495 1103 10-11 0.141562008516388 1103 12-13 0.26497287277612 1103 14-15 0.30252096432052156 1103 16-17 0.3139257863840754 1103 18-19 0.25935113225542494 1103 20-21 0.40172159605732105 1103 22-23 0.5744028964020487 1103 24-25 0.4835216882306028 1103 26-27 0.5378346551925333 1103 28-29 0.4157374799974676 1103 30-31 0.4499372094346228 1103 32-33 0.30034793171137153 1103 34-35 0.16191566865939677 1103 36-37 0.13384892692506867 1103 38-39 0.2571864670354813 1103 40-41 0.20513797277181567 1103 42-43 0.22284590675010207 1103 44-45 0.4264753949371425 1103 46-47 0.41481045259490656 1103 48-49 0.3682513535320453 1103 50-51 0.31388496581774206 1103 52-53 0.19722497628024627 1103 54-55 0.49387739161127087 1103 56-57 0.5969535951180447 1103 58-59 0.42433310893077447 1103 60-61 0.47180173114952595 1103 62-63 0.4662319223624607 1103 64-65 0.34463305044155135 1103 66-67 0.4883632988439146 1103 68-69 0.4232182975328982 1103 70-71 0.33587604142050864 1103 72-73 0.49664168012266785 1103 74-75 0.3750825019171664 1103 76-77 0.20230048720145888 1103 78-79 0.31494787942775204 1103 80-81 0.35257696410364403 1103 82-83 0.35967530700153816 1103 84-85 0.41516289299852005 1103 86-87 0.5831741211225392 1103 88-89 0.48223530776677137 1103 90-91 0.4767989101476111 1103 92-93 0.2709813106247694 1103 94-95 0.3786936621571968 1103 96-97 0.32408588310161335 1103 98-99 0.4943329222467945 1103 100-101 0.8415900681441641 1104 1 0.3114861104407076 1104 2 0.2386318068216049 1104 3 0.3091877346472849 1104 4 0.27802395136343705 1104 5 0.26544536410385433 1104 6 0.10146046742635662 1104 7 0.20654697769982278 1104 8 0.16364383305699448 1104 9 0.19336638191177968 1104 10-11 0.11472660938439105 1104 12-13 0.18710610876105704 1104 14-15 0.17693938971731882 1104 16-17 0.28191322244565953 1104 18-19 0.3975785421100184 1104 20-21 0.4069902926236395 1104 22-23 0.3638825249650992 1104 24-25 0.39532093499379783 1104 26-27 0.38361488047842585 1104 28-29 0.4297937607890745 1104 30-31 0.49952868873774037 1104 32-33 0.49220331795399375 1104 34-35 0.4655538152429628 1104 36-37 0.49863403604592094 1104 38-39 0.4976832222078329 1104 40-41 0.6147136936245658 1104 42-43 0.724680288862551 1104 44-45 0.7032428143410243 1104 46-47 1.056668086986015 1104 48-49 1.1290504953816551 1104 50-51 0.9757281050038245 1104 52-53 0.8748158603592842 1104 54-55 0.9785525127436543 1104 56-57 0.9456644425415632 1104 58-59 1.0088468986453947 1104 60-61 0.8541373861422343 1104 62-63 0.7685896948541142 1104 64-65 1.0560002244760724 1104 66-67 1.1579224048087937 1104 68-69 0.7362638749965953 1104 70-71 0.6498139197250232 1104 72-73 0.7916421190468945 1104 74-75 0.7383244176822537 1104 76-77 0.7075418848218469 1104 78-79 0.7903745458945828 1104 80-81 0.9230660541601914 1104 82-83 1.062240444427644 1104 84-85 1.2691638479263538 1104 86-87 1.3173150393520245 1104 88-89 1.2798746447984826 1104 90-91 1.3955738405085363 1104 92-93 1.4288436211647308 1104 94-95 1.5299753209053861 1104 96-97 1.6183492504854762 1104 98-99 1.5630422280827752 1104 100-101 2.0607534999749504 1105 1 0.025144037721403834 1105 2 0.09322215585935822 1105 3 0.10617899526131325 1105 4 0.07764984939428388 1105 5 0.017770834885020292 1105 6 0.255702564548109 1105 7 0.13807018691154127 1105 8 0.19255660050164636 1105 9 0.20616112421777189 1105 10-11 0.10692971635398152 1105 12-13 0.2276353730892211 1105 14-15 0.23860417450011795 1105 16-17 0.12635742562294894 1105 18-19 0.3382154460595004 1105 20-21 0.3366212779449498 1105 22-23 0.3612592615716679 1105 24-25 0.3876538092301942 1105 26-27 0.3987325085602649 1105 28-29 0.5907211986558849 1105 30-31 0.47985834929552595 1105 32-33 0.5250147904143745 1105 34-35 0.3668810603717745 1105 36-37 0.5436565566122198 1105 38-39 0.6701449537891548 1105 40-41 0.6699118291119319 1105 42-43 0.4055277776849877 1105 44-45 0.3640813280250086 1105 46-47 0.8699407450745937 1105 48-49 0.69751539514548 1105 50-51 0.5468824442971467 1105 52-53 0.5902398632710373 1105 54-55 0.42399390757115896 1105 56-57 0.5185109263139296 1105 58-59 0.47332220460662455 1105 60-61 0.42454931190914635 1105 62-63 0.5392506083329565 1105 64-65 0.6258775711439029 1105 66-67 0.3726029601920118 1105 68-69 0.4722908872681302 1105 70-71 0.40558210058440025 1105 72-73 0.6702967995609193 1105 74-75 0.5676939038659157 1105 76-77 0.4911826269872108 1105 78-79 0.7870848681310072 1105 80-81 0.6719239988121259 1105 82-83 0.6778909972051821 1105 84-85 0.6673368695700894 1105 86-87 0.6176373378146707 1105 88-89 0.7150292694763927 1105 90-91 0.8554173339467575 1105 92-93 0.8199735373278401 1105 94-95 1.0123853937404945 1105 96-97 1.011090658961809 1105 98-99 0.9712259413084894 1105 100-101 1.238562573550407 1106 1 0.09371773370293468 1106 2 0.07369494522039588 1106 3 0.06247724214104977 1106 4 0.005498970429322014 1106 5 0.22284261902758118 1106 6 0.278800946098265 1106 7 0.16306237121762734 1106 8 0.12215523455883215 1106 9 0.12674164991337022 1106 10-11 0.23118421939608425 1106 12-13 0.2745265305333717 1106 14-15 0.3025666110727556 1106 16-17 -0.018783016384610107 1106 18-19 0.3302374261956089 1106 20-21 0.41426719093824005 1106 22-23 0.21222600278802872 1106 24-25 0.37149937111846754 1106 26-27 0.3747834187880059 1106 28-29 0.26824867382602946 1106 30-31 0.3778184531841333 1106 32-33 0.18603912728281102 1106 34-35 0.19105800768198122 1106 36-37 -0.11451505197782552 1106 38-39 0.10065441679086007 1106 40-41 0.05348805016380709 1106 42-43 0.14896560083386845 1106 44-45 0.18264600589817803 1106 46-47 -0.01502426466496587 1106 48-49 -0.0320104928375855 1106 50-51 -0.12656884589355144 1106 52-53 -0.2160451570497699 1106 54-55 -0.45757728738831815 1106 56-57 -0.4570019034482371 1106 58-59 -0.06102777014267957 1106 60-61 0.028290624464759162 1106 62-63 -0.0745669372986768 1106 64-65 -0.3139385796869689 1106 66-67 -0.3806014743124777 1106 68-69 -0.35936207187381797 1106 70-71 -0.07066119595504716 1106 72-73 -0.26840411186923063 1106 74-75 -0.24087269787037258 1106 76-77 -0.28261014890678027 1106 78-79 -0.2502470944498576 1106 80-81 -0.30840149477900525 1106 82-83 0.03822154093699481 1106 84-85 0.11375427572163943 1106 86-87 0.2793410388463471 1106 88-89 0.3638400815871137 1106 90-91 0.09092823984580534 1106 92-93 0.11860518968577338 1106 94-95 0.23989122545056674 1106 96-97 0.06185359092919995 1106 98-99 0.11073006559004739 1106 100-101 0.6079803644745816 1107 1 -0.005635798039548945 1107 2 0.15257598910277892 1107 3 0.17216532865458944 1107 4 0.23044311199692658 1107 5 0.15829112500070863 1107 6 0.16984870133406105 1107 7 0.07544655806781009 1107 8 0.23652351292746232 1107 9 0.012760656675965265 1107 10-11 0.12857600299307137 1107 12-13 0.09552334287882758 1107 14-15 0.17455993785203816 1107 16-17 0.3025802272773248 1107 18-19 0.16757767107898047 1107 20-21 0.1910509368791935 1107 22-23 0.13887419893304553 1107 24-25 0.237623838601408 1107 26-27 0.19525441708990598 1107 28-29 0.2932821888854633 1107 30-31 0.2692952011806824 1107 32-33 0.3392133758006963 1107 34-35 0.2604014104287202 1107 36-37 0.09889841958650436 1107 38-39 0.31174268800961613 1107 40-41 -0.04549607114581278 1107 42-43 0.04198255200615364 1107 44-45 0.20318850299354096 1107 46-47 0.31635625787205157 1107 48-49 0.3972891168981718 1107 50-51 0.4638503157830982 1107 52-53 0.39263151944798125 1107 54-55 0.3152859413780291 1107 56-57 0.25562955006265753 1107 58-59 0.3022993070462334 1107 60-61 0.3411395534724164 1107 62-63 0.30584984108485713 1107 64-65 0.40319729781176505 1107 66-67 0.3041290646096826 1107 68-69 0.270823824989165 1107 70-71 0.24310083222739465 1107 72-73 0.3642097648549303 1107 74-75 0.4766236527120462 1107 76-77 0.5603263661228617 1107 78-79 0.5277714952364398 1107 80-81 0.400850151182798 1107 82-83 0.5090619496717643 1107 84-85 0.5553621048395208 1107 86-87 0.5122051613476089 1107 88-89 0.816932404404163 1107 90-91 0.7397187185898098 1107 92-93 0.2914594811029403 1107 94-95 0.20411799579048306 1107 96-97 0.3708386059737414 1107 98-99 0.5900564873733494 1107 100-101 0.35919435440516523 1108 1 0.1870084479354155 1108 2 0.1806475385201729 1108 3 0.13425598536096572 1108 4 0.11489773093051525 1108 5 0.17528687280055522 1108 6 0.14172293259952795 1108 7 0.13534384258367282 1108 8 0.14995835687121684 1108 9 0.21858822920685128 1108 10-11 0.21784208429193086 1108 12-13 0.28962247386366613 1108 14-15 0.1366109341319799 1108 16-17 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-3.4668882754598727 2316 98-99 -4.44408191888941 2316 100-101 -4.670345193994184 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3715.0 3 772.0 4 664.0 5 694.0 6 856.0 7 1110.0 8 1349.0 9 1598.0 10 2124.0 11 3105.0 12 4469.0 13 6540.0 14 6340.0 15 6038.0 16 6979.0 17 8315.0 18 7515.0 19 6391.0 20 6958.0 21 7416.0 22 8167.0 23 9380.0 24 10506.0 25 12417.0 26 14325.0 27 16602.0 28 19546.0 29 23031.0 30 27647.0 31 33621.0 32 41131.0 33 53111.0 34 73576.0 35 131458.0 36 143428.0 37 191317.0 38 120454.0 39 13565.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.899680164012445 16.080235543129977 13.63412052920983 17.38596376364775 2 11.930455577022292 24.6515610630033 40.0650893979572 23.352893962017205 3 26.483335999157337 30.0978032309787 30.774467387869592 12.644393381994373 4 11.358929813386503 15.23244505529343 40.63172146586849 32.77690366545158 5 11.508019345537123 42.70542927543979 33.678812578891204 12.107738800131884 6 23.20281643440185 36.52646258569743 20.40598394788426 19.86473703201646 7 22.75593822722192 40.837369526755914 19.298043705976557 17.108648540045607 8 18.64931949070304 30.281810335158752 22.720574251342242 28.348295922795963 9 23.96014380377768 25.18487524456591 23.273701191667495 27.58127975998892 10-11 21.24266956909306 24.163967585670832 28.10296786752608 26.49039497771003 12-13 34.85289076127745 22.509886039288624 24.52654539921848 18.11067780021545 14-15 29.125136861202623 24.376144574200108 31.32530414277946 15.17341442181781 16-17 17.494566412652247 28.673102608853394 34.96982207287273 18.862508905621624 18-19 15.89624116107366 35.40067117072521 32.97324924760308 15.729838420598044 20-21 18.363672188201694 32.11389409655501 26.83442613036701 22.68800758487629 22-23 16.849094300453434 30.17587194555657 24.697970077965863 28.277063676024135 24-25 20.4186548193015 29.847974316182007 29.108115663040873 20.62525520147562 26-27 25.73035423576917 24.096140439546822 31.500351498907452 18.673153825776552 28-29 18.890276443069897 26.039746923125705 36.369702971262996 18.700273662541402 30-31 17.35936764522966 35.39694114720394 31.42057884557696 15.82311236198945 32-33 16.68015282031322 33.904726787110455 27.98291526257029 21.432205130006032 34-35 19.23640109043638 27.546479019272397 28.76143329587037 24.455686594420854 36-37 23.430725721205327 27.766411167037557 31.834819269255703 16.96804384250141 38-39 27.399924250182146 22.50215465604144 33.53781537721303 16.56010571656339 40-41 18.831553349316863 26.913995058003557 38.01095663630776 16.24349495637182 42-43 16.286482336750836 33.4307126490391 32.41183072950221 17.870974284707856 44-45 17.173962365298966 35.17037834140155 27.276313345809307 20.37934594749018 46-47 17.046298637283897 30.30264723233202 24.241862999308157 28.40919113107592 48-49 20.607131077522645 28.70961244314364 27.753058272958548 22.930198206375167 50-51 25.91550262048215 30.344766753811893 28.391815373005237 15.347915252700725 52-53 18.248064651921577 30.004180165628647 34.387767453416636 17.35998772903314 54-55 14.179454424914702 29.247228686130423 36.06444275557999 20.508874133374885 56-57 19.331922522999147 35.18002527701849 26.52264644505188 18.96540575493049 58-59 17.009446508563578 30.402523640763263 29.06840330248176 23.5196265481914 60-61 17.788909690508454 25.708076468146107 32.229400521694174 24.273613319651265 62-63 27.611125646626274 24.524615159987025 28.84607281411167 19.018186379275033 64-65 23.922355697362683 26.501644341846664 33.44434478736283 16.131655173427827 66-67 17.16045479388935 27.893992534478407 35.637645248021485 19.307907423610754 68-69 15.70368416847263 34.610311083485826 32.30349622720403 17.38250852083751 70-71 17.114942230121706 32.716216103607856 26.77629324775486 23.392548418515577 72-73 16.64822930232262 29.792607834074502 25.502820500127193 28.05634236347569 74-75 19.16271998376726 29.877994688090748 29.216040025074484 21.743245303067507 76-77 23.63614713003713 24.70702038692175 31.18386060271352 20.4729718803276 78-79 18.615408960789992 25.433030707884868 35.70960694946003 20.24195338186511 80-81 17.084253333679534 33.61471830720263 31.43763046776598 17.86339789135186 82-83 16.56418181319306 31.98032094826336 28.308995018011203 23.146502220532376 84-85 18.422757359498696 26.623460625530438 28.759621396692616 26.19416061827825 86-87 21.71840059266344 26.773335632909284 31.165003067190085 20.343260707237196 88-89 24.974150646601363 22.130672769237556 32.81152758652796 20.08364899763313 90-91 18.65569158686127 24.989707799527537 36.54221272508062 19.812387888530566 92-93 16.439801097444118 30.67505802141462 32.885582015444605 19.999558865696663 94-95 16.42454349173984 32.401585127057686 28.908031751555598 22.265839629646873 96-97 16.09824739569804 27.99216577348773 26.368710538465834 29.540876292348393 98-99 19.38004329952794 26.9596173329471 28.134547131737126 25.525792235787836 100-101 23.604169739545636 27.09125237724451 29.142800299700646 20.161777583509213 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 23378.0 1 13778.5 2 4815.5 3 4917.0 4 3308.0 5 3135.5 6 6753.5 7 12655.0 8 16157.0 9 14502.0 10 9357.0 11 5469.0 12 4684.5 13 4362.0 14 3693.0 15 3121.0 16 2953.0 17 2519.0 18 1977.0 19 1772.0 20 1715.0 21 1811.0 22 2017.5 23 2308.0 24 2649.0 25 3102.0 26 3748.0 27 4433.0 28 5032.0 29 5798.5 30 6575.0 31 7186.0 32 8157.5 33 9408.5 34 10287.5 35 10960.0 36 12012.5 37 12563.5 38 12721.5 39 12841.0 40 12561.0 41 12551.0 42 12751.5 43 12913.0 44 15566.0 45 18701.0 46 30086.5 47 59528.0 48 206364.0 49 209413.5 50 67672.0 51 36937.5 52 17520.5 53 10505.5 54 7744.5 55 5916.5 56 4599.0 57 3659.0 58 3053.0 59 2624.5 60 2229.0 61 1849.0 62 1325.0 63 836.5 64 517.0 65 405.0 66 327.0 67 236.0 68 180.5 69 144.5 70 111.5 71 81.0 72 73.5 73 68.5 74 47.0 75 41.0 76 41.5 77 30.5 78 20.5 79 19.0 80 15.0 81 11.0 82 5.5 83 4.0 84 4.5 85 0.5 86 1.0 87 1.0 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28297446701116014 2 0.08594472448479451 3 0.08935522942466731 4 0.10465378015495384 5 0.10670008311887753 6 0.08097513157240843 7 0.09110920339374474 8 0.08828335644356443 9 0.09305806335938635 10-11 0.0869191544676153 12-13 0.09076815289975747 14-15 0.09973290874170883 16-17 0.08823463494442338 18-19 0.09914825075201635 20-21 0.10163304720820938 22-23 0.1146904089780081 24-25 0.12818626424007618 26-27 0.13101211119025646 28-29 0.1223884058422924 30-31 0.1153237884668416 32-33 0.10869766458366018 34-35 0.3798328073035476 36-37 0.5800294472740808 38-39 0.5628794795764348 40-41 0.5260460262258085 42-43 0.5516735347739956 44-45 0.572916108399489 46-47 0.56312308707214 48-49 0.586265799164134 50-51 0.5762291703410798 52-53 0.5794447892843884 54-55 0.5682388444819492 56-57 0.5409060834638257 58-59 0.5353031110626062 60-61 0.48263517049114196 62-63 0.5604921261185238 64-65 0.5920636575619177 66-67 0.6059492848171142 68-69 0.6081904737776019 70-71 0.6043901968446008 72-73 0.5963998709854703 74-75 0.5925021510541871 76-77 0.5754496263548231 78-79 0.5527454077550985 80-81 0.5490425738203795 82-83 0.5617588850961909 84-85 0.5709185269347063 86-87 0.5615639990996267 88-89 0.5754983478539641 90-91 0.5896275826048657 92-93 0.5986410599459581 94-95 0.5976666299631372 96-97 0.5945971755172518 98-99 0.5980564019562656 100-101 0.5738905383823099 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1026241.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.90873300600036 #Duplication Level Percentage of deduplicated Percentage of total 1 75.3959341774969 17.272253258102538 2 12.834529712908022 5.880456289011766 3 4.545743934356533 3.124117023174583 4 2.313602863394941 2.120068411177305 5 1.277543950345227 1.4633456630944894 6 0.799994641824153 1.0996118193468245 7 0.5475832652210552 0.8781107175052125 8 0.3698480898138322 0.6778200913859458 9 0.2708607273448653 0.5584568476099122 >10 1.3858765928456231 5.665146275358834 >50 0.11345911522310398 1.8419175296953354 >100 0.09639759413239539 4.42783294098947 >500 0.016634983058245224 2.563343371606496 >1k 0.021326901356724645 10.387633151450471 >5k 0.004265380271344928 7.3853564353825565 >10k+ 0.0063980704070173925 34.65453017510828 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 64486 6.283709187218207 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 53025 5.166914983907288 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 41142 4.008999835321333 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 24227 2.3607515193799506 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 23185 2.259215915170023 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 22431 2.185743894465335 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 18213 1.7747293277115221 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 17533 1.7084680888797075 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 15309 1.491754860700362 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15298 1.490682987719259 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13597 1.324932447641441 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 12970 1.2638356877185768 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 12630 1.2307050683026697 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 10446 1.017889560054607 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 10159 0.9899234195476501 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9766 0.9516283212227926 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 9257 0.902029835097214 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 9254 0.9017375061023678 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 8612 0.8391791012052724 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8321 0.8108231887051872 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 6789 0.6615405153370407 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 6355 0.619250254082618 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 6355 0.619250254082618 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5663 0.5518196992714187 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 5209 0.5075805780513545 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 4718 0.4597360658948532 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 4589 0.44716591911646486 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4583 0.4465812611267723 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 4178 0.40711684682252997 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 4127 0.4021472539101439 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 3981 0.38792057616096026 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3663 0.3569337027072588 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3627 0.35342575476910393 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3464 0.3375425460491249 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 3314 0.3229260963068129 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 2850 0.27771254510392784 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 2744 0.26738358728602735 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2709 0.2639730823461546 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 2684 0.26153700738910257 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2467 0.24039187676189122 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2373 0.2312322349233757 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 2365 0.23045269093711906 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2119 0.2064817133597274 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 2082 0.20287632242329043 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 2050 0.19975814647826387 No Hit TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA 1902 0.18533658273251605 No Hit GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1888 0.1839723807565669 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1744 0.16994058900394743 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 1725 0.1680891720365879 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1691 0.16477611009499718 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1657 0.16146304815340645 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1531 0.1491852303698644 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1507 0.14684659841109446 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1453 0.14158467650386217 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1439 0.14022047452791303 No Hit TTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCG 1436 0.1399281455330668 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1429 0.13924604454509223 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1425 0.13885627255196392 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1369 0.13339946464816743 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1340 0.13057361769798712 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 1339 0.13047617469970504 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1334 0.12998895970829466 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1320 0.12862475773234552 No Hit GTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 1308 0.12745544175296056 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1292 0.12589635378044728 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1280 0.12472703780106233 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 1232 0.12004977388352248 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 1222 0.11907534390070168 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 1221 0.11897790090241961 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 1168 0.11381342199346937 No Hit CATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1147 0.11176711902954568 No Hit CAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTA 1140 0.11108501804157113 No Hit ACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGAT 1047 0.1020228192013377 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 9.744299828207995E-5 0.0 5 0.0 0.0 0.0 9.744299828207995E-5 0.0 6 0.0 0.0 0.0 9.744299828207995E-5 0.0 7 0.0 0.0 0.0 9.744299828207995E-5 0.0 8 0.0 0.0 0.0 9.744299828207995E-5 0.0 9 0.0 0.0 0.0 9.744299828207995E-5 0.0 10-11 0.0 0.0 0.0 2.9232899484623985E-4 0.0 12-13 0.0 0.0 0.0 4.8721499141039966E-4 0.0 14-15 0.0 0.0 0.0 6.333794888335196E-4 0.0 16-17 0.0 0.0 0.0 0.0011693159793849592 0.0 18-19 0.0 0.0 0.0 0.001607809471654319 0.0 20-21 0.0 0.0 0.0 0.0024360749570519983 0.0 22-23 0.0 0.0 0.0 0.004920871413245037 0.0 24-25 0.0 0.0 0.0 0.008477540850540954 0.0 26-27 0.0 0.0 0.0 0.013495855262068073 0.0 28-29 0.0 0.0 0.0 0.02650449553272574 0.0 30-31 0.0 0.0 0.0 0.053983421048272286 0.0 32-33 0.0 0.0 0.0 0.1008047817228117 0.0 34-35 0.0 0.0 0.0 0.20828440882794585 0.0 36-37 0.0 0.0 0.0 0.3650701930638125 0.0 38-39 0.0 0.0 0.0 0.518055700366678 0.0 40-41 0.0 0.0 0.0 0.682539481466829 0.0 42-43 0.0 0.0 0.0 0.8451718455996204 0.0 44-45 0.0 0.0 0.0 1.0159894215881065 0.0 46-47 0.0 0.0 0.0 1.2571608423362544 0.0 48-49 0.0 0.0 0.0 1.5692707658337564 0.0 50-51 0.0 0.0 0.0 1.8352901511438346 0.0 52-53 0.0 0.0 0.0 2.0445002684554603 0.0 54-55 0.0 0.0 0.0 2.2643316725798326 0.0 56-57 0.0 0.0 0.0 2.4899122136028478 0.0 58-59 0.0 0.0 0.0 2.9122301681573823 0.0 60-61 0.0 0.0 0.0 3.6440758067549437 0.0 62-63 0.0 0.0 0.0 4.172898958431791 0.0 64-65 0.0 0.0 0.0 4.502597343119209 0.0 66-67 0.0 0.0 0.0 4.895731119688261 0.0 68-69 0.0 0.0 0.0 5.312348658843293 0.0 70-71 0.0 0.0 0.0 5.845215694948847 0.0 72-73 0.0 0.0 0.0 6.534088971304011 0.0 74-75 0.0 0.0 0.0 7.185641579317139 0.0 76-77 0.0 0.0 0.0 7.684549730521388 0.0 78-79 0.0 0.0 0.0 8.207769909797015 0.0 80-81 0.0 0.0 0.0 8.795984568926793 0.0 82-83 0.0 0.0 0.0 9.401251752755933 0.0 84-85 0.0 0.0 0.0 10.61826607979997 0.0 86-87 0.0 0.0 0.0 11.97647531135474 0.0 88-89 0.0 0.0 0.0 12.754898703131136 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 25 5.368723E-5 75.73059 2 ATCACGC 40 5.4830615E-4 47.33162 3 TCACGCA 50 0.0016441676 37.865295 4 AAAGTAC 340 0.0 32.07476 1 GTAAGCG 30 0.0039268113 31.707506 94-95 AAGTACT 355 0.0 30.665556 2 GCTTATG 265 0.0 30.417086 1 GTCTTAG 115 3.9183687E-6 28.861223 1 AGTACTC 395 0.0 27.560183 3 CGCCGAC 35 0.008340469 27.177864 94-95 GTCTAAG 70 0.00846489 27.09421 1 CCTAGAC 70 0.00853776 27.046638 3 TGTATCG 70 0.008539791 27.04532 9 CCCTGCG 215 6.002665E-11 26.416359 9 AGGGCGA 45 9.1400347E-4 26.294062 10-11 CCGTATC 70 1.7620201E-5 23.781796 92-93 GTACTGG 160 1.8884602E-6 23.707434 1 CGCAGAA 580 0.0 23.664656 9 CATGGGT 565 0.0 23.456377 4 GTACCCT 305 0.0 23.274439 6 >>END_MODULE