##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938972_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1026241 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.986201097013275 33.0 31.0 34.0 30.0 34.0 2 32.17926978165947 33.0 31.0 34.0 30.0 34.0 3 32.27624310468983 34.0 31.0 34.0 30.0 34.0 4 35.77243551953196 37.0 35.0 37.0 35.0 37.0 5 35.71512344566237 37.0 35.0 37.0 33.0 37.0 6 35.82552831157594 37.0 35.0 37.0 35.0 37.0 7 35.79623792072233 37.0 35.0 37.0 33.0 37.0 8 35.84189776085734 37.0 35.0 37.0 35.0 37.0 9 37.40038451007122 39.0 37.0 39.0 34.0 39.0 10-11 37.31993264739958 39.0 37.0 39.0 33.5 39.0 12-13 37.228665099133636 39.0 37.0 39.0 33.5 39.0 14-15 38.07669982002278 40.0 37.0 41.0 33.0 41.0 16-17 38.07419845825688 40.0 37.0 41.0 33.0 41.0 18-19 38.4071811592014 40.0 37.5 41.0 34.0 41.0 20-21 38.42795892972508 40.0 38.0 41.0 34.0 41.0 22-23 38.43370027118387 40.0 38.0 41.0 34.0 41.0 24-25 38.309926225906 40.0 37.5 41.0 34.0 41.0 26-27 37.786498980259026 39.5 36.5 41.0 32.5 41.0 28-29 37.47423022467432 39.0 36.0 41.0 32.5 41.0 30-31 37.4406864469457 39.0 36.0 41.0 33.0 41.0 32-33 37.316342360127884 39.0 35.0 41.0 32.5 41.0 34-35 37.35473343980605 39.5 35.0 41.0 33.0 41.0 36-37 37.06058810747183 39.0 35.0 41.0 32.0 41.0 38-39 36.69365041934594 39.0 35.0 41.0 30.5 41.0 40-41 36.41642362758846 38.0 35.0 40.5 30.5 41.0 42-43 36.508163774396074 39.0 35.0 40.0 31.0 41.0 44-45 36.487241301019935 39.0 35.0 41.0 30.5 41.0 46-47 36.18822138269665 39.0 35.0 41.0 30.0 41.0 48-49 35.98837992245486 39.0 35.0 41.0 29.5 41.0 50-51 35.82823868857315 39.0 35.0 40.0 28.5 41.0 52-53 35.2164545170189 38.0 34.0 40.0 27.5 41.0 54-55 35.11622075126603 38.0 34.0 40.0 27.5 41.0 56-57 35.16807065786691 38.0 34.0 40.0 27.5 41.0 58-59 35.07113582482087 38.0 34.0 40.0 27.0 41.0 60-61 34.566559901621545 37.5 33.5 40.0 26.0 41.0 62-63 34.015909518329515 37.0 33.0 39.0 26.0 41.0 64-65 33.43177869525774 36.0 32.0 39.0 23.0 40.0 66-67 32.982499724723525 36.0 32.0 39.0 22.5 40.0 68-69 32.74817172574473 35.0 32.0 38.0 23.0 39.5 70-71 32.21063911888143 35.0 31.5 37.0 22.5 39.0 72-73 31.53695184659354 35.0 31.0 36.5 21.0 39.0 74-75 31.062876069071493 34.5 31.0 36.0 20.0 37.0 76-77 28.815437601888835 32.5 28.0 34.5 13.5 36.0 78-79 29.73066803996332 34.0 29.0 35.0 14.0 36.0 80-81 29.983446870666832 34.0 30.0 35.0 13.5 36.0 82-83 29.787257086785658 34.0 30.0 35.0 9.0 36.0 84-85 29.514505364724272 34.0 30.0 35.0 4.5 35.5 86-87 28.80752815371828 34.0 29.0 35.0 2.0 35.0 88-89 27.989282244618956 33.0 27.5 35.0 2.0 35.0 90-91 27.303601688102503 33.0 25.5 35.0 2.0 35.0 92-93 27.176215430878322 33.0 25.5 35.0 2.0 35.0 94-95 26.9637663083038 33.0 25.5 35.0 2.0 35.0 96-97 26.117122099000138 32.0 24.5 34.0 2.0 35.0 98-99 25.157962895655114 32.0 21.0 34.0 2.0 35.0 100-101 20.94847019364847 27.5 2.0 32.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19005975393117325 1101 2 0.15497782288356632 1101 3 0.16308557417978875 1101 4 0.09024246757277155 1101 5 0.1114736475410183 1101 6 0.07582129452727315 1101 7 0.11701619734180468 1101 8 0.11733889504910167 1101 9 0.11667761913647468 1101 10-11 0.1438129604474483 1101 12-13 0.21086848508934253 1101 14-15 0.16717013112187118 1101 16-17 0.13806254580182298 1101 18-19 0.3256725773222655 1101 20-21 0.19907770555511917 1101 22-23 0.18724365671554466 1101 24-25 0.23970525874532456 1101 26-27 0.10909800432155947 1101 28-29 0.1590442499492326 1101 30-31 0.0613926444703381 1101 32-33 0.3272753681265428 1101 34-35 0.2669445121546232 1101 36-37 0.2232094732063885 1101 38-39 0.4130473605418956 1101 40-41 0.5153689119786975 1101 42-43 0.1819127442868691 1101 44-45 0.23111412541245357 1101 46-47 0.26501774638225584 1101 48-49 0.16003877047018022 1101 50-51 0.14339437161850555 1101 52-53 0.21343554195433967 1101 54-55 -0.03338685759630522 1101 56-57 -0.06311281587905881 1101 58-59 0.05352246558658891 1101 60-61 0.23519790832891374 1101 62-63 0.041346379655628596 1101 64-65 0.05988876241040941 1101 66-67 0.23377084513814594 1101 68-69 0.3924056063853527 1101 70-71 0.3191231250212141 1101 72-73 0.0037467990276986995 1101 74-75 0.10445011886418243 1101 76-77 0.15144925291831157 1101 78-79 0.32448959815689804 1101 80-81 0.44546429487973427 1101 82-83 0.3157138527706813 1101 84-85 0.406631879431945 1101 86-87 0.5871034175572838 1101 88-89 0.6600034322281871 1101 90-91 0.7744583066404189 1101 92-93 0.655365029118272 1101 94-95 0.8770436335527627 1101 96-97 0.9973777940116122 1101 98-99 0.809386482054439 1101 100-101 0.9667514386694656 1102 1 0.09129301611476848 1102 2 0.028754335777051665 1102 3 0.18001728965774078 1102 4 0.15917759311039958 1102 5 0.012610761425470685 1102 6 0.1759180838150698 1102 7 0.14367491574907376 1102 8 0.18038333479296398 1102 9 0.280821995422464 1102 10-11 0.2674500583071904 1102 12-13 0.3665149542032964 1102 14-15 0.12762113074368386 1102 16-17 0.33599022946109613 1102 18-19 0.20843254629841823 1102 20-21 0.23882701488984281 1102 22-23 0.16958121416414684 1102 24-25 0.18558017621803913 1102 26-27 -0.026749927528520345 1102 28-29 0.24962556256581792 1102 30-31 0.18107804723555887 1102 32-33 0.10110251912423251 1102 34-35 0.37717533217141863 1102 36-37 0.33913387500372494 1102 38-39 0.23390545206225255 1102 40-41 0.25286803061928254 1102 42-43 0.13187877188784114 1102 44-45 0.11386127461538109 1102 46-47 0.21164582553154077 1102 48-49 0.09644628542124423 1102 50-51 0.30697628035454017 1102 52-53 0.4607594142950333 1102 54-55 0.35362126373002667 1102 56-57 0.34520497347924817 1102 58-59 0.30504641612790095 1102 60-61 0.21553397868382262 1102 62-63 0.28385963209619547 1102 64-65 0.2126748196250574 1102 66-67 0.05747813125626067 1102 68-69 0.20355752710787556 1102 70-71 0.3985304028064789 1102 72-73 0.41347316096835485 1102 74-75 0.43489099086515637 1102 76-77 0.37001356651560613 1102 78-79 0.5318686596212991 1102 80-81 0.6680155579478928 1102 82-83 0.7096029873718912 1102 84-85 0.6161742776269215 1102 86-87 0.6552373072687736 1102 88-89 0.8107335183129649 1102 90-91 0.7272494917643542 1102 92-93 0.6290700717760664 1102 94-95 0.9248309406953013 1102 96-97 0.8435933969575977 1102 98-99 0.9548139894418348 1102 100-101 1.017402362654618 1103 1 -0.020564182698951328 1103 2 0.137818010058119 1103 3 0.0884120676177389 1103 4 -0.1082539569298504 1103 5 -0.023838492415087842 1103 6 -0.037188237986903516 1103 7 0.15695599424754647 1103 8 0.1119010923976731 1103 9 0.09344278630412362 1103 10-11 0.09635421329830507 1103 12-13 -0.05247821478987191 1103 14-15 0.30423515518379673 1103 16-17 -0.07022331805679727 1103 18-19 -0.018671528631749368 1103 20-21 -0.016173566197700495 1103 22-23 0.037058820772195133 1103 24-25 -0.10904519449429273 1103 26-27 0.01706622498328869 1103 28-29 0.18053102607996863 1103 30-31 0.19057237194727605 1103 32-33 0.2568038487386062 1103 34-35 0.1283030724295955 1103 36-37 0.03380573271905973 1103 38-39 0.19324223748599678 1103 40-41 0.05416622322182718 1103 42-43 -0.041520977598864306 1103 44-45 0.09775869329541109 1103 46-47 0.1162301757680595 1103 48-49 0.11256393197367487 1103 50-51 -5.277119320652446E-4 1103 52-53 0.32913340223424115 1103 54-55 0.27359474892090674 1103 56-57 0.05696510915242925 1103 58-59 0.14094621941900698 1103 60-61 0.10613779320068772 1103 62-63 -0.014656872942047983 1103 64-65 0.07102504754050187 1103 66-67 0.10605382113629247 1103 68-69 0.08194451419051774 1103 70-71 0.3305410087301226 1103 72-73 0.348019030296836 1103 74-75 0.21479009685122108 1103 76-77 -0.027541776691911934 1103 78-79 0.2390069971944193 1103 80-81 0.3445951475709599 1103 82-83 0.3517423795112826 1103 84-85 0.45269067936071394 1103 86-87 0.39071442318067184 1103 88-89 0.7945276325418646 1103 90-91 0.5846710557076591 1103 92-93 0.4522096231764863 1103 94-95 0.4957677398929725 1103 96-97 0.4038607833119414 1103 98-99 0.46786908945345473 1103 100-101 0.7420465003422336 1104 1 0.12374232772496185 1104 2 0.13364715933732185 1104 3 0.08285485866196751 1104 4 -0.017612892035437255 1104 5 0.05414549648548217 1104 6 0.04204614969238918 1104 7 0.055394280430427045 1104 8 0.21421433013830438 1104 9 0.1413508306076352 1104 10-11 0.05992457285218222 1104 12-13 0.14557189108722213 1104 14-15 0.22481128070327827 1104 16-17 0.14736694510504122 1104 18-19 0.23968141355229733 1104 20-21 0.11950487148897793 1104 22-23 0.07829200515676149 1104 24-25 0.0644802877335664 1104 26-27 0.14684541397976858 1104 28-29 0.12213965126223059 1104 30-31 0.13082889639535722 1104 32-33 0.19355272627018394 1104 34-35 0.03128850656847959 1104 36-37 -0.1049628385932806 1104 38-39 0.017270555592581616 1104 40-41 -0.08138642520414407 1104 42-43 -0.24593976698160702 1104 44-45 -0.17979129589206622 1104 46-47 -0.3214516833865062 1104 48-49 -0.374337926709849 1104 50-51 -0.20059472816797808 1104 52-53 -0.09083144100557661 1104 54-55 0.01580652022639839 1104 56-57 -0.0175824242004623 1104 58-59 -0.030735781047745547 1104 60-61 -0.032227181930508664 1104 62-63 -0.0314430483276098 1104 64-65 -0.09207919589329805 1104 66-67 0.2142368675273758 1104 68-69 0.12167250963427279 1104 70-71 0.07799203656220044 1104 72-73 -0.0119041900795267 1104 74-75 0.048867638704251704 1104 76-77 0.08493148639926673 1104 78-79 0.30521175855880855 1104 80-81 0.40994454196257735 1104 82-83 0.38591851873376726 1104 84-85 0.17187873377018903 1104 86-87 -0.04716869879101537 1104 88-89 0.0056228876638932945 1104 90-91 0.162960685748061 1104 92-93 0.07016141002631926 1104 94-95 0.1084802702162122 1104 96-97 -0.037942681406047285 1104 98-99 0.3047637754727539 1104 100-101 1.0600032117078015 1105 1 -0.013074765570980418 1105 2 0.06400091214463544 1105 3 0.06448684489733125 1105 4 -0.025542654339027138 1105 5 0.06078482413594344 1105 6 0.07758687076410808 1105 7 0.053993207549815736 1105 8 0.11020198828893513 1105 9 -0.018936042942179654 1105 10-11 0.02343050182037132 1105 12-13 -0.032796577638350755 1105 14-15 0.10425748984468441 1105 16-17 0.20856125892054678 1105 18-19 0.08399067945214966 1105 20-21 0.07500090298882611 1105 22-23 0.29272284902658186 1105 24-25 0.22772078835603082 1105 26-27 0.3044412969969059 1105 28-29 0.20220295287237633 1105 30-31 0.2490985765578202 1105 32-33 0.22671989546189053 1105 34-35 0.3308533984738986 1105 36-37 0.26503000611200633 1105 38-39 0.3803764291382592 1105 40-41 0.37398604304163996 1105 42-43 0.2642373989426403 1105 44-45 0.052797142309763956 1105 46-47 0.2961577864185614 1105 48-49 0.33109606466243235 1105 50-51 0.5699193570089776 1105 52-53 0.6499478450366354 1105 54-55 0.620580404641494 1105 56-57 0.6468120227945207 1105 58-59 0.5909228410823815 1105 60-61 0.4620635753312854 1105 62-63 0.3923401379706277 1105 64-65 0.38737673286809127 1105 66-67 0.41618937374172305 1105 68-69 0.13974014578133165 1105 70-71 0.26905598338966996 1105 72-73 0.34193857542119943 1105 74-75 0.4951600172813819 1105 76-77 0.3018527243893345 1105 78-79 0.5680101198722411 1105 80-81 0.4295399162506648 1105 82-83 0.5968663264424592 1105 84-85 0.5363892992039112 1105 86-87 0.4993965529471396 1105 88-89 0.6133695281909866 1105 90-91 0.6624436846851083 1105 92-93 0.5957348676053478 1105 94-95 0.6526833440977313 1105 96-97 0.560130352232111 1105 98-99 0.704620669578528 1105 100-101 1.0447041162528627 1106 1 -0.08190096858663765 1106 2 -0.11822499748189585 1106 3 -0.0015016600205441932 1106 4 0.04981867120265804 1106 5 0.060093435828221686 1106 6 0.030283886371449853 1106 7 0.023926834272273823 1106 8 0.042578199721489796 1106 9 -0.061164838363382046 1106 10-11 0.06969339771533356 1106 12-13 0.044302236781000204 1106 14-15 -0.07360064929871868 1106 16-17 -0.003833229211615219 1106 18-19 0.00810029155324088 1106 20-21 -0.09121786429588497 1106 22-23 0.03173578700604196 1106 24-25 -0.01465289800618308 1106 26-27 0.18247138140640828 1106 28-29 0.1390671762328921 1106 30-31 0.10103626744110983 1106 32-33 -0.005745895847063309 1106 34-35 0.07751068630786762 1106 36-37 0.049743711231890586 1106 38-39 0.36819897681399283 1106 40-41 0.2991357436404485 1106 42-43 0.36670415218314645 1106 44-45 0.29291930761770146 1106 46-47 0.1808161804738262 1106 48-49 0.23861536341432554 1106 50-51 0.22101776260742412 1106 52-53 0.5796396662135592 1106 54-55 0.5341298118511801 1106 56-57 0.45007597681419753 1106 58-59 0.43606388429714826 1106 60-61 0.4207756692158 1106 62-63 0.18461402393364068 1106 64-65 0.3143751456810193 1106 66-67 0.3208829985403199 1106 68-69 0.2977945189626823 1106 70-71 0.2589617140969338 1106 72-73 0.34880436192048947 1106 74-75 0.3002937257680216 1106 76-77 -0.021169905050580695 1106 78-79 0.3069148405513644 1106 80-81 0.44862824561603176 1106 82-83 0.4449893574887973 1106 84-85 0.29425910176745873 1106 86-87 0.34359971289230984 1106 88-89 0.4043568306944145 1106 90-91 0.7411657586095473 1106 92-93 0.36385825937602334 1106 94-95 0.3857383184863039 1106 96-97 0.13920713841877586 1106 98-99 0.2651622370460025 1106 100-101 0.8148385461881702 1107 1 0.0038946044464012175 1107 2 -0.005039428321708783 1107 3 0.017540851970402116 1107 4 -0.12278835355379414 1107 5 -0.02283622723371792 1107 6 0.009829273209717826 1107 7 -0.03425530890405781 1107 8 0.003098949267176465 1107 9 -0.08308517679100902 1107 10-11 0.04062764861651402 1107 12-13 0.037481549796758884 1107 14-15 0.06413730762326253 1107 16-17 0.17102004930597303 1107 18-19 0.10134067645508082 1107 20-21 0.04805880624363823 1107 22-23 -0.15181800094045173 1107 24-25 -0.17729179930805827 1107 26-27 0.06904748293468543 1107 28-29 0.11076706004734405 1107 30-31 0.009757630684767093 1107 32-33 0.12557951303666215 1107 34-35 -0.1651904785266467 1107 36-37 -0.18404989558433016 1107 38-39 -0.11619343239743074 1107 40-41 0.04264275945955376 1107 42-43 -0.06883968551458963 1107 44-45 0.014392778586206134 1107 46-47 -0.2721093118251545 1107 48-49 -0.27163875521409153 1107 50-51 -0.25087576377265464 1107 52-53 -0.06564737165101064 1107 54-55 -0.11816112865587058 1107 56-57 -0.0150728300496894 1107 58-59 -0.278980653492944 1107 60-61 -0.4220714723220169 1107 62-63 -0.33050797088269945 1107 64-65 -0.33473409851715274 1107 66-67 -0.008432996335599796 1107 68-69 -0.06567704537133778 1107 70-71 -0.2763884810351911 1107 72-73 0.0439975576186491 1107 74-75 -0.1084559390813844 1107 76-77 -0.53571246172827 1107 78-79 0.020571110101816004 1107 80-81 0.10833569638613483 1107 82-83 -0.0688262947887317 1107 84-85 -0.23019944382791735 1107 86-87 -0.19678518894580677 1107 88-89 0.23387093681053983 1107 90-91 0.1965922191213565 1107 92-93 0.29846069629323324 1107 94-95 0.4079386938549696 1107 96-97 -0.07210605608773335 1107 98-99 -0.24314651857708114 1107 100-101 0.05367132838497213 1108 1 -0.019159650012884555 1108 2 0.06634733112023383 1108 3 0.055170062125476704 1108 4 0.08519393480260362 1108 5 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-1.3876136224824265 2316 56-57 -1.5594480296002686 2316 58-59 -1.294351807471223 2316 60-61 -1.4002520559998857 2316 62-63 -1.3988551060436052 2316 64-65 -1.3133657170289794 2316 66-67 -1.4873838957782723 2316 68-69 -1.5889271635861135 2316 70-71 -1.2033103299546823 2316 72-73 -1.2690569909221594 2316 74-75 -1.7522899776618353 2316 76-77 -1.6061676593389187 2316 78-79 -1.6809210230585414 2316 80-81 -1.7354033662212167 2316 82-83 -1.7983367441665408 2316 84-85 -1.98762878776623 2316 86-87 -2.0766232043925363 2316 88-89 -2.49219774496337 2316 90-91 -2.708977010279966 2316 92-93 -3.154940049773348 2316 94-95 -3.263515570162067 2316 96-97 -3.463466879913369 2316 98-99 -3.588674362703319 2316 100-101 -4.342575301988745 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 7.0 4 21.0 5 92.0 6 300.0 7 448.0 8 540.0 9 847.0 10 1363.0 11 1870.0 12 2628.0 13 3656.0 14 4047.0 15 5043.0 16 6110.0 17 7462.0 18 7592.0 19 6281.0 20 6574.0 21 7114.0 22 7831.0 23 9051.0 24 10229.0 25 12191.0 26 14425.0 27 16958.0 28 20373.0 29 24532.0 30 30028.0 31 36179.0 32 44114.0 33 56544.0 34 80660.0 35 132266.0 36 155934.0 37 196535.0 38 110185.0 39 6210.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.58082254228782 16.751270028692424 14.257582264500535 17.410325164519218 2 12.221788059529876 25.380198218547108 39.50631479350367 22.89169892841935 3 26.54610369299219 29.928155277366624 30.89137931538498 12.634361714256205 4 11.666557855318585 15.790442985614488 39.776426784741595 32.76657237432533 5 10.99828206654824 43.325248749552976 33.62355332560929 12.052915858289492 6 23.412434311238783 37.027754689200684 19.518514656888584 20.041296342671945 7 22.605216513470033 40.446639726925746 19.288354294946313 17.659789464657912 8 19.116269959980162 30.810501626810854 22.436737569440314 27.63649084376867 9 23.7804765157502 25.083094516785042 22.779931809389804 28.356497158074955 10-11 21.46440261108258 24.516122431280763 27.812862670659232 26.206612286977425 12-13 34.740329026028 22.649358191691814 24.644601024515683 17.965711757764502 14-15 28.86708090149426 24.683806107698132 31.080279800960703 15.368833189846912 16-17 17.455344679486835 29.082464165874523 34.69197439344235 18.77021676119629 18-19 16.132175580589745 35.34671680433738 32.612514994041355 15.908592621031511 20-21 18.487811342559887 32.145714310771055 26.71102596758461 22.655448379084444 22-23 17.125704391073832 30.094782804428977 24.65985085374683 28.11966195075036 24-25 20.745322005259972 30.1294725118174 28.813797149012753 20.31140833390987 26-27 25.609773922499684 24.5039420564955 31.560520384588024 18.325763636416788 28-29 19.072128281758378 26.131824785795928 35.98623520206267 18.809811730383018 30-31 17.262709246658435 35.70233502656783 31.163245280591983 15.871710446181744 32-33 16.797418929861504 33.80005281410507 28.040928008138437 21.361600247894987 34-35 19.336379143428033 27.609276192155956 28.66489933673969 24.389445327676317 36-37 23.43048977762186 28.011167631409695 31.52253990527977 17.03580268568867 38-39 27.35396947215827 22.602589871968227 33.718914836336175 16.32452581953733 40-41 18.96814152048379 27.03278809930756 37.62621488833844 16.37285549187021 42-43 16.262227925907954 33.4955278361626 32.45926623187431 17.78297800605513 44-45 17.31706936805959 35.090222911688926 27.024436874169293 20.568270846082186 46-47 17.268061956358096 30.53247368910833 24.137761348848244 28.061703005685324 48-49 20.906777076295093 28.543107404805312 27.826656363673568 22.723459155226024 50-51 25.829937027247592 30.492746562077492 28.195276434548532 15.482039976126384 52-53 18.39197615374946 30.192469410206762 34.29447858738834 17.12107584865543 54-55 14.112084783203946 29.498480376441794 35.7402890743987 20.64914576595556 56-57 19.41956129213313 35.18915147611526 26.123201080447966 19.26808615130364 58-59 17.20098748782571 30.572219669755775 28.760217512366744 23.466575330051775 60-61 17.96081240087993 26.133738705488845 31.736352186549148 24.169096707082076 62-63 27.515271753905758 24.921631468631634 28.704125054446276 18.858971723016328 64-65 23.807025082899955 26.897977757574516 33.025899475462296 16.269097684063233 66-67 17.034001448031173 28.44323980896902 35.13521941180311 19.38753933119669 68-69 15.861523612500372 34.793766087472974 31.80261063479713 17.542099665229525 70-71 17.295498815580356 33.040679528492824 26.33621147469259 23.32761018123423 72-73 16.91439924929914 29.934342907757532 25.041778685513442 28.109479157429885 74-75 19.393446568593536 30.26862111336421 28.52059116718198 21.817341150860276 76-77 23.334188621223888 25.27180593875488 30.97607210174479 20.41793333827644 78-79 18.486465154349858 25.565657156220766 35.14455682881197 20.8033208606174 80-81 16.877760681945077 33.936180682705135 30.89167164437983 18.294386990969958 82-83 16.76053675501174 32.4138287205442 27.765456651995002 23.060177872449064 84-85 18.395727709183323 27.0928076348538 28.033863390762985 26.47760126519989 86-87 21.344493533930702 27.20130565564552 30.4170437828884 21.03715702753538 88-89 24.56528706442466 22.578601119046045 32.581659290357464 20.274452526171828 90-91 18.422749324828 25.494543632608668 35.828573523481325 20.25413351908201 92-93 16.075512477088715 30.94516785043669 32.406130723679915 20.57318894879468 94-95 16.575492501274066 32.8140271144887 27.989965320036912 22.62051506420032 96-97 15.976231611216512 28.932936543897753 25.51433051039867 29.576501334487066 98-99 18.905793083690867 27.299727841705803 27.659925884855507 26.134553189747827 100-101 22.75749337386966 27.727675787340196 28.77665068599938 20.73818015279077 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 21428.0 1 13029.0 2 4877.5 3 4695.5 4 3283.5 5 3215.0 6 6861.0 7 12757.0 8 15966.5 9 14350.5 10 9504.0 11 5531.5 12 4585.0 13 4093.0 14 3381.5 15 2619.5 16 1957.5 17 1607.5 18 1521.0 19 1532.5 20 1571.5 21 1656.0 22 1828.0 23 2091.5 24 2448.0 25 2885.0 26 3518.0 27 4186.5 28 4824.0 29 5660.5 30 6477.5 31 7189.5 32 8118.0 33 9343.5 34 10503.5 35 11351.5 36 12111.5 37 12654.5 38 12929.5 39 13012.0 40 12879.5 41 12761.0 42 12801.5 43 12995.0 44 15726.0 45 18653.0 46 29570.0 47 60065.0 48 207164.5 49 206227.0 50 64987.5 51 44272.5 52 25293.5 53 10807.0 54 6632.0 55 4981.5 56 4045.5 57 3388.5 58 2853.0 59 2413.0 60 2054.5 61 1708.5 62 1244.0 63 755.5 64 411.5 65 277.5 66 215.5 67 163.5 68 102.0 69 64.5 70 52.5 71 39.0 72 39.0 73 42.5 74 38.0 75 22.5 76 13.0 77 10.5 78 10.0 79 6.0 80 1.5 81 3.0 82 3.5 83 1.0 84 0.0 85 0.5 86 1.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3917208530939614 2 0.0 3 0.0 4 0.0 5 7.795439862566395E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0011205944802439192 16-17 0.0019001384665005587 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0021924674613467985 36-37 0.005944022895206877 38-39 0.012521425279247274 40-41 0.001461644974231199 42-43 3.897719931283197E-4 44-45 4.872149914103997E-4 46-47 2.4360749570519986E-4 48-49 1.9488599656415986E-4 50-51 3.410504939872798E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 4.8721499141039966E-5 60-61 0.0022899104596288784 62-63 0.0 64-65 3.897719931283197E-4 66-67 0.0019488599656415989 68-69 0.0015103664733722392 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.12131653286118953 78-79 9.744299828207993E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14309504297723438 88-89 0.4276285979609078 90-91 0.32677509473895505 92-93 0.0 94-95 0.0 96-97 3.897719931283197E-4 98-99 0.0 100-101 9.744299828207993E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1026241.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.473465691867034 #Duplication Level Percentage of deduplicated Percentage of total 1 73.4953516852625 15.781999129251863 2 13.706593043655541 5.886561109506412 3 5.037244619963017 3.2450129858495296 4 2.405479294115379 2.0661590837873245 5 1.4131503709788644 1.5172618004331908 6 0.8837612328338972 1.1386449907836484 7 0.581733126861687 0.8744278439040879 8 0.41672208428210933 0.7158773903900159 9 0.2982338441306708 0.5763702798084221 >10 1.4829125295504488 5.663118745680527 >50 0.1230614391329611 1.8664633421134706 >100 0.10171868031541872 4.435495430855935 >500 0.01952635381054913 2.8455740687061777 >1k 0.023159163821814086 10.854077690417153 >5k 0.004086911262673074 6.721452284144911 >10k+ 0.007265620022529909 35.81150382436732 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 64718 6.30631596281965 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 54939 5.35342088261919 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 37458 3.6500198296501503 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 26003 2.5338102843289247 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 22850 2.2265725107455268 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 21807 2.1249394635373173 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 19254 1.8761674889231674 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 17384 1.6939490821356775 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 15290 1.4899034437330023 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15236 1.48464152182577 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 14159 1.3796954126759697 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 13532 1.3185986527531057 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12774 1.244736860055289 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 11227 1.0939925417129115 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 10439 1.0172074590666325 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10185 0.9924569375029841 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 9768 0.9518232072193569 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 9602 0.9356476695045315 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 9287 0.9049531250456764 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8622 0.8401535311880932 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 6864 0.6688487402081967 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 6845 0.6669973232408372 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 6350 0.6187630390912077 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6065 0.5909917845808148 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 5527 0.5385674515050559 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 4996 0.48682521941727136 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 4691 0.45710510494123696 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4602 0.4484326780941319 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 4285 0.4175432476387126 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 4161 0.40546031585173464 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3948 0.38470495721765163 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 3855 0.3756427583774182 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 3626 0.35332831177082186 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3447 0.33588601507832955 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3404 0.3316959661522001 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 3122 0.30421704063665356 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 2878 0.28044094905582606 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 2738 0.26679892929633486 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2567 0.2501361765900992 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2562 0.2496489615986888 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 2369 0.23084246293024738 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2307 0.2248009970367584 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 2196 0.21398482422744755 No Hit TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA 2123 0.2068714853528557 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 2047 0.19946581748341766 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2022 0.19702974252636565 No Hit GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2013 0.19615275554182693 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 1884 0.1835826087634386 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1814 0.176761598883693 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1738 0.16935593101425492 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1717 0.16730962805033125 No Hit TTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCG 1611 0.15698067023243079 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1611 0.15698067023243079 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1572 0.15318039329942967 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1571 0.15308295030114757 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1566 0.1525957353097372 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1551 0.15113409033550598 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1546 0.1506468753440956 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1526 0.148698015378454 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1519 0.14801591439047943 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1403 0.13671252658975816 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1384 0.13486110962239864 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 1382 0.13466622362583447 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1376 0.134081565636142 No Hit GTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 1365 0.13300969265503912 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 1357 0.13223014866878247 No Hit CAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTA 1337 0.13028128870314087 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 1319 0.12852731473406345 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 1294 0.12609123977701145 No Hit CATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1263 0.12307050683026696 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1227 0.11956255889211209 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 1210 0.11790602792131674 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1092 0.10640775412403128 No Hit ACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGAT 1077 0.1049461091498001 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 9.744299828207995E-5 0.0 5 0.0 0.0 0.0 9.744299828207995E-5 0.0 6 0.0 0.0 0.0 9.744299828207995E-5 0.0 7 0.0 0.0 0.0 9.744299828207995E-5 0.0 8 0.0 0.0 0.0 9.744299828207995E-5 0.0 9 0.0 0.0 0.0 9.744299828207995E-5 0.0 10-11 0.0 0.0 0.0 2.9232899484623985E-4 0.0 12-13 0.0 0.0 0.0 4.8721499141039966E-4 0.0 14-15 0.0 0.0 0.0 6.333794888335196E-4 0.0 16-17 0.0 0.0 0.0 0.0011693159793849592 0.0 18-19 0.0 0.0 0.0 0.001607809471654319 0.0 20-21 0.0 0.0 0.0 0.0024360749570519983 0.0 22-23 0.0 0.0 0.0 0.004920871413245037 0.0 24-25 0.0 0.0 0.0 0.008477540850540954 0.0 26-27 0.0 0.0 0.0 0.013593298260350151 0.0 28-29 0.0 0.0 0.0 0.026699381529289905 0.0 30-31 0.0 0.0 0.0 0.05490912953195205 0.0 32-33 0.0 0.0 0.0 0.10294852768501746 0.0 34-35 0.0 0.0 0.0 0.2127180652497805 0.0 36-37 0.0 0.0 0.0 0.3720373674409812 0.0 38-39 0.0 0.0 0.0 0.5269717347094883 0.0 40-41 0.0 0.0 0.0 0.6938428692675502 0.0 42-43 0.0 0.0 0.0 0.859349801849663 0.0 44-45 0.0 0.0 0.0 1.032408566798637 0.0 46-47 0.0 0.0 0.0 1.2750416325210159 0.0 48-49 0.0 0.0 0.0 1.590805668454096 0.0 50-51 0.0 0.0 0.0 1.8594072932186494 0.0 52-53 0.0 0.0 0.0 2.0708098779916218 0.0 54-55 0.0 0.0 0.0 2.2921516485893667 0.0 56-57 0.0 0.0 0.0 2.5204605935642794 0.0 58-59 0.0 0.0 0.0 2.9453120660741483 0.0 60-61 0.0 0.0 0.0 3.6817375255909672 0.0 62-63 0.0 0.0 0.0 4.214458397199098 0.0 64-65 0.0 0.0 0.0 4.546495413845285 0.0 66-67 0.0 0.0 0.0 4.9408472278928635 0.0 68-69 0.0 0.0 0.0 5.3601931709997945 0.0 70-71 0.0 0.0 0.0 5.897786192522029 0.0 72-73 0.0 0.0 0.0 6.594260022743196 0.0 74-75 0.0 0.0 0.0 7.251366881658402 0.0 76-77 0.0 0.0 0.0 7.754854853781909 0.0 78-79 0.0 0.0 0.0 8.282801018474217 0.0 80-81 0.0 0.0 0.0 8.87510828353184 0.0 82-83 0.0 0.0 0.0 9.483932136798277 0.0 84-85 0.0 0.0 0.0 10.7021157798217 0.0 86-87 0.0 0.0 0.0 12.065245882789714 0.0 88-89 0.0 0.0 0.0 12.846738729011996 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGAGT 60 1.5029345E-6 47.45028 4 GTAAGCG 50 3.0609954E-8 37.960224 94-95 ACGTGGA 70 2.0658494E-4 33.89306 8 GTCGGGA 70 2.0658494E-4 33.89306 2 CCCTGCG 155 3.6379788E-11 33.674393 9 AAGTACT 325 0.0 32.12019 4 AGTACTC 370 0.0 32.061 5 GGTCGGG 75 3.0235673E-4 31.76352 1 GTATAGG 80 4.41365E-4 29.778301 1 TAGGACG 80 4.520951E-4 29.656424 4 ACCACTC 695 0.0 29.357727 94-95 AGTACCC 200 2.3646862E-11 28.470167 5 CTAGGTT 125 7.450246E-6 26.572157 4 GACGTGG 95 0.0012339755 24.973831 7 CATGGGG 1315 0.0 24.537025 4 TGTAGGA 215 1.6825652E-9 24.276888 2 GCGTTGT 235 0.0 24.229929 94-95 ATGGGGA 715 0.0 23.891048 5 AAAGTAC 430 0.0 23.173391 3 GTAGGAC 205 2.6984708E-8 23.146477 3 >>END_MODULE