##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938971_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2235463 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.876928850980757 33.0 31.0 34.0 30.0 34.0 2 31.894016586273178 33.0 31.0 34.0 30.0 34.0 3 31.97542701444846 34.0 31.0 34.0 30.0 34.0 4 35.45244139580928 37.0 35.0 37.0 33.0 37.0 5 35.532857846450604 37.0 35.0 37.0 33.0 37.0 6 35.67736616530893 37.0 35.0 37.0 33.0 37.0 7 35.62583679533054 37.0 35.0 37.0 33.0 37.0 8 35.63913873770221 37.0 35.0 37.0 33.0 37.0 9 37.188028162398574 39.0 37.0 39.0 34.0 39.0 10-11 37.08236257992192 39.0 37.0 39.0 33.0 39.0 12-13 37.00522531573996 39.0 37.0 39.0 33.0 39.0 14-15 38.05557506431553 40.0 38.0 41.0 33.0 41.0 16-17 38.054228587098066 40.0 38.0 41.0 33.0 41.0 18-19 38.06736166064927 40.0 38.0 41.0 33.0 41.0 20-21 38.18266059424826 40.0 38.0 41.0 33.0 41.0 22-23 38.134559373158936 40.0 38.0 41.0 33.0 41.0 24-25 37.9792201883905 40.0 38.0 41.0 33.0 41.0 26-27 37.796724436951095 40.0 37.5 41.0 32.5 41.0 28-29 37.7344395769467 40.0 37.0 41.0 33.0 41.0 30-31 37.63881419643269 40.0 37.0 41.0 33.0 41.0 32-33 37.417395635713945 40.0 37.0 41.0 32.0 41.0 34-35 36.996708511838484 39.0 36.0 41.0 31.0 41.0 36-37 36.64588968817645 39.0 35.5 41.0 30.0 41.0 38-39 36.37233763206996 39.0 35.0 40.5 30.0 41.0 40-41 36.3806184669574 39.0 35.0 41.0 30.0 41.0 42-43 36.18225016473097 39.0 35.0 41.0 30.0 41.0 44-45 36.083719345835746 39.0 35.0 41.0 30.0 41.0 46-47 35.789667285926896 39.0 35.0 41.0 27.5 41.0 48-49 35.440074382801235 39.0 35.0 41.0 26.5 41.0 50-51 34.25364656001911 38.0 34.0 39.5 24.5 40.5 52-53 34.45606078025 38.0 33.5 39.5 25.0 40.5 54-55 35.047485464979744 39.0 35.0 40.0 26.0 41.0 56-57 34.89418590242826 38.5 34.5 40.0 25.0 41.0 58-59 34.68274156181516 38.0 34.0 40.0 25.0 41.0 60-61 34.18439379224796 38.0 34.0 40.0 23.0 41.0 62-63 33.888295623770105 37.5 33.5 40.0 22.0 41.0 64-65 33.4949019509605 37.0 33.0 39.5 22.0 41.0 66-67 32.91028771220995 36.0 32.0 39.0 20.5 40.5 68-69 32.41080259436188 36.0 32.0 39.0 18.5 40.0 70-71 31.73893931592695 35.0 31.0 37.5 15.5 40.0 72-73 31.260641307863292 35.0 31.0 37.0 12.0 39.0 74-75 30.844171878487813 35.0 31.0 37.0 8.0 39.0 76-77 30.427702896446952 34.5 30.0 36.0 7.0 38.5 78-79 29.97811594287179 34.0 30.0 36.0 2.0 37.0 80-81 29.415407904313334 34.0 29.0 35.0 2.0 37.0 82-83 28.89835081144264 34.0 29.0 35.0 2.0 36.5 84-85 28.444779895708407 34.0 28.5 35.0 2.0 36.0 86-87 28.144803112375378 34.0 27.5 35.0 2.0 36.0 88-89 27.566688869375156 33.5 26.5 35.0 2.0 35.0 90-91 26.975054832041508 33.0 25.0 35.0 2.0 35.0 92-93 26.405617091403435 33.0 24.5 35.0 2.0 35.0 94-95 25.76972846341004 32.0 23.0 35.0 2.0 35.0 96-97 25.015085018181914 32.0 18.5 34.5 2.0 35.0 98-99 24.181346772458323 32.0 2.0 34.0 2.0 35.0 100-101 20.968599569753557 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13026117114196012 1101 2 0.2051867825925413 1101 3 0.25977697076590545 1101 4 0.19663127443408968 1101 5 -0.018275049148058997 1101 6 0.15927717067081204 1101 7 0.22451626085941712 1101 8 0.12938843938055555 1101 9 0.32040231052319257 1101 10-11 0.14434623243229794 1101 12-13 0.16237868476102335 1101 14-15 0.3192881906064784 1101 16-17 0.35977202302139943 1101 18-19 0.49522738679763023 1101 20-21 0.3554931954514231 1101 22-23 0.4326375403260485 1101 24-25 0.3586825138378842 1101 26-27 0.42785422217634306 1101 28-29 0.3816391924968414 1101 30-31 0.31666188192775024 1101 32-33 0.34658394330269715 1101 34-35 0.28578503406975386 1101 36-37 0.22980695685095753 1101 38-39 0.603361749089089 1101 40-41 0.250866923324665 1101 42-43 0.30814184781950615 1101 44-45 0.30953421210132603 1101 46-47 0.35993865520684665 1101 48-49 0.26824415065609486 1101 50-51 0.21383989180649365 1101 52-53 0.2925223038815048 1101 54-55 0.4374370555455869 1101 56-57 0.5304808776442869 1101 58-59 0.5476632677996562 1101 60-61 0.6194414781535471 1101 62-63 0.5714408388523324 1101 64-65 0.49432634793669905 1101 66-67 0.533832160816452 1101 68-69 0.37759610425975865 1101 70-71 0.33047077693671056 1101 72-73 0.32603384363839893 1101 74-75 0.07482104649834298 1101 76-77 0.1469475612359048 1101 78-79 0.08722698558050368 1101 80-81 -0.014779904234742958 1101 82-83 0.0930045065727434 1101 84-85 -0.018321676490465677 1101 86-87 0.04268405259839625 1101 88-89 -0.08444709867100642 1101 90-91 -0.08327289984883635 1101 92-93 -0.07582760623898821 1101 94-95 -0.25585571140319274 1101 96-97 -0.5169604417517881 1101 98-99 -0.42495466198397125 1101 100-101 0.09139362073225499 1102 1 0.2623552216470415 1102 2 0.2734123148437959 1102 3 0.3544554025673037 1102 4 0.1894703915873066 1102 5 0.32915146558816843 1102 6 0.2683701698125134 1102 7 0.2753428833668039 1102 8 0.344676601318028 1102 9 0.34380348154940066 1102 10-11 0.31770273975138963 1102 12-13 0.378074894140191 1102 14-15 0.5439434896154793 1102 16-17 0.628265257711405 1102 18-19 0.5236039385728972 1102 20-21 0.5427249479778808 1102 22-23 0.5083501602074705 1102 24-25 0.6059668415284207 1102 26-27 0.6326953203416039 1102 28-29 0.5688282749193903 1102 30-31 0.5759166893678369 1102 32-33 0.4108439539305806 1102 34-35 0.4176414843787839 1102 36-37 0.5067110985791246 1102 38-39 0.6794067527580125 1102 40-41 0.5834485555470792 1102 42-43 0.42715144905921676 1102 44-45 0.5807902777265426 1102 46-47 0.6701388852523351 1102 48-49 0.7197088894113008 1102 50-51 0.7286664704779255 1102 52-53 0.7278832754727773 1102 54-55 0.7309347379527011 1102 56-57 0.6647460902941376 1102 58-59 0.6500382904462825 1102 60-61 0.8526587783391264 1102 62-63 0.7844089596213166 1102 64-65 0.5670201162594779 1102 66-67 0.6420336345905397 1102 68-69 0.7533048535197295 1102 70-71 0.686399484753057 1102 72-73 0.6901338763521458 1102 74-75 0.665949348972017 1102 76-77 0.7610139591819589 1102 78-79 0.7671937448700525 1102 80-81 0.7635347992068731 1102 82-83 0.7571373929347942 1102 84-85 0.7175781275768074 1102 86-87 0.7363431352564476 1102 88-89 0.4626787667101766 1102 90-91 0.648818539152618 1102 92-93 0.8019385182220127 1102 94-95 0.6148081724576464 1102 96-97 0.6987327364178135 1102 98-99 0.5692185371336187 1102 100-101 0.754056370454375 1103 1 0.2513007806134979 1103 2 0.2990939133364563 1103 3 0.34287304247411754 1103 4 0.3772873318184651 1103 5 0.12774220253202628 1103 6 0.2569058068269783 1103 7 0.209384860129866 1103 8 0.18725430005348187 1103 9 0.32971627885491017 1103 10-11 0.4036394432709187 1103 12-13 0.46409336965532333 1103 14-15 0.46893603219827895 1103 16-17 0.49054446152355524 1103 18-19 0.5169143285486157 1103 20-21 0.5653563212376582 1103 22-23 0.44663342500808767 1103 24-25 0.508706309578578 1103 26-27 0.5389380045209293 1103 28-29 0.470873683982326 1103 30-31 0.5040778082824815 1103 32-33 0.6099499134452984 1103 34-35 0.5670960309489388 1103 36-37 0.5589763834524604 1103 38-39 0.7808960650519907 1103 40-41 0.665289461462784 1103 42-43 0.5277487032193235 1103 44-45 0.6362692138268642 1103 46-47 0.5181000840274308 1103 48-49 0.6103916489954955 1103 50-51 0.5806031098070221 1103 52-53 0.608748911694299 1103 54-55 0.6692316904178028 1103 56-57 0.5235049683965158 1103 58-59 0.5161292728241236 1103 60-61 0.47146664412071715 1103 62-63 0.46768584313834793 1103 64-65 0.47788049924977116 1103 66-67 0.46306801139589027 1103 68-69 0.5262926960417431 1103 70-71 0.37680126962450444 1103 72-73 0.5069047816637173 1103 74-75 0.384032665313768 1103 76-77 0.3322398452930386 1103 78-79 0.42441332710365387 1103 80-81 0.35240762475616805 1103 82-83 0.5111330109942074 1103 84-85 0.49217651262119233 1103 86-87 0.5700386017131756 1103 88-89 0.6812348974622999 1103 90-91 0.7796630538918734 1103 92-93 0.7584322766470066 1103 94-95 0.7784065603339592 1103 96-97 0.7019167703707723 1103 98-99 0.5297508645652123 1103 100-101 0.7257896913272113 1104 1 0.2831892674410881 1104 2 0.3754208994947561 1104 3 0.3409212677657365 1104 4 0.3546842545785722 1104 5 0.38249890641387907 1104 6 0.3460209164867436 1104 7 0.3143908910493636 1104 8 0.39847254579138536 1104 9 0.41288321029365704 1104 10-11 0.5054953300367231 1104 12-13 0.5145600304381688 1104 14-15 0.5431954058652266 1104 16-17 0.5747649651788862 1104 18-19 0.5892885801997707 1104 20-21 0.5633870789270787 1104 22-23 0.6450120637854297 1104 24-25 0.6134484877701922 1104 26-27 0.6056196565515819 1104 28-29 0.686052478680999 1104 30-31 0.4829957914547478 1104 32-33 0.6185163933005597 1104 34-35 0.6746960007943485 1104 36-37 0.7405240681677583 1104 38-39 0.9030166687323202 1104 40-41 0.7509524213203989 1104 42-43 0.7147053688563219 1104 44-45 0.8429072703998273 1104 46-47 0.8145694219501465 1104 48-49 0.7651766687798514 1104 50-51 0.7611551411972286 1104 52-53 0.7093981548874524 1104 54-55 0.8932803496414365 1104 56-57 0.8570571064310215 1104 58-59 0.8113565722960558 1104 60-61 0.8185492787499626 1104 62-63 0.7148726398375231 1104 64-65 0.7316686705573048 1104 66-67 0.6449154033909466 1104 68-69 0.6580650692821095 1104 70-71 0.5153588869700165 1104 72-73 0.5236612685795627 1104 74-75 0.5056646358063936 1104 76-77 0.6844253181318365 1104 78-79 0.666627373262255 1104 80-81 0.6535573607195744 1104 82-83 0.8326757128461395 1104 84-85 0.793410828553121 1104 86-87 0.7433342234127167 1104 88-89 0.7764829741873633 1104 90-91 0.7483514576104469 1104 92-93 0.8268510357485468 1104 94-95 0.8115991415675055 1104 96-97 0.9156963181989077 1104 98-99 0.8949745234211015 1104 100-101 0.8293826851639103 1105 1 0.0902052520475145 1105 2 0.27394708945249846 1105 3 0.24995077062980897 1105 4 0.20410277396940302 1105 5 0.2249949846971191 1105 6 0.18008741458254462 1105 7 0.2516569767538712 1105 8 0.2578377831607881 1105 9 0.2621670803726204 1105 10-11 0.3055296589454457 1105 12-13 0.21075340995813718 1105 14-15 0.30913990635182387 1105 16-17 0.420389195833593 1105 18-19 0.3752452791141323 1105 20-21 0.3980928279638718 1105 22-23 0.30716072018082485 1105 24-25 0.312858452708042 1105 26-27 0.08661321738151884 1105 28-29 0.24497821714137302 1105 30-31 0.3467880137905368 1105 32-33 0.43863979961707855 1105 34-35 0.3619200919114931 1105 36-37 0.49226762122091117 1105 38-39 0.48495766300417387 1105 40-41 0.4983904182008061 1105 42-43 0.4227394444643622 1105 44-45 0.4788826335752461 1105 46-47 0.4625057990393273 1105 48-49 0.3995162427903196 1105 50-51 0.36379835607174726 1105 52-53 0.43885824669784057 1105 54-55 0.6809403845702704 1105 56-57 0.5022901652812166 1105 58-59 0.5040372934475457 1105 60-61 0.5360095838661962 1105 62-63 0.4971722545538455 1105 64-65 0.4398239527653729 1105 66-67 0.6339189318250575 1105 68-69 0.6359595851000677 1105 70-71 0.6369475488758205 1105 72-73 0.4719240480435438 1105 74-75 0.5420475020147464 1105 76-77 0.43441565271093197 1105 78-79 0.565516072052187 1105 80-81 0.5304518943130212 1105 82-83 0.5101606147921096 1105 84-85 0.48567382576680984 1105 86-87 0.5848545385720954 1105 88-89 0.7087161530220953 1105 90-91 0.7959197710278296 1105 92-93 0.895217446300073 1105 94-95 0.8880975040007932 1105 96-97 0.8838249816156214 1105 98-99 0.8208334127198142 1105 100-101 0.9818367022017114 1106 1 0.16015850975166757 1106 2 0.20438917097439457 1106 3 0.2428673425197161 1106 4 0.3575084900491987 1106 5 0.2267504104663587 1106 6 0.25391333076678535 1106 7 0.2242617411148231 1106 8 0.22840797082411513 1106 9 0.23147756476462433 1106 10-11 0.23522004062613178 1106 12-13 0.2977117007086747 1106 14-15 0.3954066204335689 1106 16-17 0.34545212226227306 1106 18-19 0.28870240424401317 1106 20-21 0.33547716027094765 1106 22-23 0.39208486139659726 1106 24-25 0.3878387765188407 1106 26-27 0.40226734788617335 1106 28-29 0.3292868166772607 1106 30-31 0.31037910787208745 1106 32-33 0.39808260838373855 1106 34-35 0.24597484991839025 1106 36-37 0.4309981572782675 1106 38-39 0.5617328183579673 1106 40-41 0.5793341491052857 1106 42-43 0.49086999824896793 1106 44-45 0.5209591363074892 1106 46-47 0.25476219073058104 1106 48-49 0.39687135715782773 1106 50-51 0.2454600409515777 1106 52-53 0.3036588524147774 1106 54-55 0.19237350528328534 1106 56-57 0.18737865740700244 1106 58-59 0.3692129481547255 1106 60-61 0.6227006842484002 1106 62-63 0.5328933214785181 1106 64-65 0.351840257571709 1106 66-67 0.38114541677665414 1106 68-69 0.2934353089008326 1106 70-71 0.26066469871707554 1106 72-73 0.20046098321410355 1106 74-75 0.41690715231787223 1106 76-77 0.31639397206002684 1106 78-79 0.4995102263284643 1106 80-81 0.3952427820749129 1106 82-83 0.6141026872367945 1106 84-85 0.6378388473225165 1106 86-87 0.677069495466025 1106 88-89 0.8475595668649447 1106 90-91 0.9351150602758196 1106 92-93 0.8800978033227906 1106 94-95 0.9538369068911869 1106 96-97 1.0517799714651659 1106 98-99 1.2237773123536293 1106 100-101 1.2868077031831184 1107 1 0.01936216071262109 1107 2 0.12135796637156915 1107 3 0.06812667487128721 1107 4 -0.049826447992607825 1107 5 -0.03752127863881327 1107 6 0.014448590747001333 1107 7 -0.04241015829686745 1107 8 0.05052578732420443 1107 9 0.13889099398178217 1107 10-11 0.07583280752727717 1107 12-13 0.1561020958805912 1107 14-15 0.2169398022664737 1107 16-17 0.22511206858925448 1107 18-19 0.234671508622327 1107 20-21 0.20586019720665405 1107 22-23 0.17752892210640425 1107 24-25 0.19945746389721108 1107 26-27 0.2159392647403493 1107 28-29 0.15019549530946819 1107 30-31 0.23867586182593215 1107 32-33 0.3618508326641745 1107 34-35 0.30341761988442784 1107 36-37 0.3561565101098054 1107 38-39 0.36641095470336893 1107 40-41 0.08912031671759024 1107 42-43 0.33048729770401764 1107 44-45 0.1400392820269758 1107 46-47 0.40943528121470507 1107 48-49 0.45527346371147104 1107 50-51 0.46781214738062005 1107 52-53 0.40588270675509364 1107 54-55 0.45834085295434335 1107 56-57 0.37909864823723893 1107 58-59 0.48539951046550556 1107 60-61 0.4491308432989314 1107 62-63 0.45616913199334874 1107 64-65 0.39407193611172175 1107 66-67 0.45783254098457604 1107 68-69 0.5469340841893242 1107 70-71 0.4338314937105814 1107 72-73 0.5205514224905876 1107 74-75 0.47656480620418407 1107 76-77 0.42432587909657116 1107 78-79 0.5276745419975448 1107 80-81 0.536365243259155 1107 82-83 0.7130619939229987 1107 84-85 0.8434997430189597 1107 86-87 0.9939555013615831 1107 88-89 1.0340804517470445 1107 90-91 0.9886728285349875 1107 92-93 0.9782320182490736 1107 94-95 0.9400230829755536 1107 96-97 1.1556698944441415 1107 98-99 0.9701763739480214 1107 100-101 1.2146886407913549 1108 1 0.16521038494848028 1108 2 0.27552396054586836 1108 3 0.27972159620889414 1108 4 0.19020913646090776 1108 5 0.3377736573014829 1108 6 0.3487522345028111 1108 7 0.29047356585060413 1108 8 0.3487012194485857 1108 9 0.48014244464389577 1108 10-11 0.40741285717642484 1108 12-13 0.44609137443077174 1108 14-15 0.3969362284498672 1108 16-17 0.5418117834524878 1108 18-19 0.5318045775700853 1108 20-21 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quality scores pass #Quality Count 2 5816.0 3 1385.0 4 1408.0 5 1459.0 6 1874.0 7 2341.0 8 2892.0 9 3683.0 10 4822.0 11 6703.0 12 9399.0 13 14044.0 14 12531.0 15 13348.0 16 16685.0 17 22826.0 18 19972.0 19 14561.0 20 14550.0 21 15841.0 22 17292.0 23 19739.0 24 22188.0 25 25643.0 26 29942.0 27 34851.0 28 41176.0 29 49074.0 30 59125.0 31 72215.0 32 89641.0 33 116082.0 34 156311.0 35 222398.0 36 317784.0 37 419089.0 38 317457.0 39 39312.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.32847245903405 18.309582353680206 14.20365418095016 19.15829100633559 2 14.339393890553598 24.344797667287633 35.99430163460888 25.321506807549888 3 26.180289986670623 24.416621556335528 30.675114723519563 18.727973733474286 4 11.612191421872845 15.555534659796141 42.037820130497 30.79445378783401 5 11.354988538298294 40.839303762635524 34.47529408814427 13.330413610921912 6 27.45155697107875 37.51898827091037 16.673469771388827 18.355984986622055 7 25.19482077294535 35.28530820196461 21.475826379791187 18.044044645298857 8 23.97089976810585 27.998633968442523 20.336190571249155 27.694275692202474 9 22.457132293798825 21.76944069331345 25.08069096802753 30.692736044860197 10-11 25.423517158436976 24.027831798933583 26.954351141143146 23.5942999014863 12-13 31.119924484084233 23.184867634546293 25.54373294435535 20.15147493701412 14-15 24.169052046294304 25.37535182065018 30.60795458741455 19.84764154564096 16-17 19.5785737112207 28.730990231156284 30.02831827829165 21.66211777933137 18-19 18.804393024980676 32.18802580203904 29.025369200035627 19.982211972944654 20-21 22.49759405563886 26.12168483247913 26.612766052684588 24.767955059197423 22-23 20.178070502954263 26.49579938774834 28.73226838433789 24.593861724959506 24-25 24.665978072437607 26.281062852803807 27.73946782142755 21.31349125333104 26-27 23.357113596833877 23.098970516801014 33.41218053898449 20.131735347380626 28-29 19.360907052299396 30.61444770709725 29.764645786783078 20.25999945382028 30-31 20.756410919295174 26.598854744920764 28.55406566000791 24.090668675776154 32-33 21.545246867118276 27.019718617523548 29.715862382612755 21.71917213274542 34-35 24.708495853410444 25.117299375106285 28.621114829772885 21.553089941710386 36-37 22.977647664008632 24.02365419781333 32.646078537348245 20.352619600829794 38-39 21.84709429004212 26.800625308081962 31.288455480949356 20.063824920926567 40-41 20.91192513860289 28.337234797934386 29.877214495792327 20.873625567670402 42-43 19.653992518465657 28.949354039075825 26.713656945944926 24.68299649651359 44-45 21.912853306894327 25.387938986842894 27.538325358410837 25.16088234785194 46-47 24.528231930640843 27.88446521941465 27.554077578514537 20.033225271429973 48-49 23.22403677704199 26.589167583433248 28.530300493424566 21.656495146100195 50-51 18.769684185544982 27.970837178215398 32.51803854605701 20.741440090182618 52-53 21.581816763943305 30.20232236872711 26.64197725634714 21.57388361098245 54-55 19.116798425189774 26.211107265969446 29.386997029253536 25.285097279587244 56-57 23.148018963027667 25.698178175263543 27.631416653017975 23.52238620869081 58-59 26.39126250754766 25.425229664452687 26.694157214123464 21.48935061387619 60-61 22.166125698477693 26.488541990620725 31.704897455559582 19.640434855342 62-63 21.01137291778312 26.905359752925804 30.341771739484575 21.741495589806505 64-65 20.009686590477475 30.851835620132828 28.209306768252258 20.929171021137442 66-67 21.628814203911507 26.579715839055183 26.47935683929164 25.312113117741674 68-69 19.647036317610667 27.634779610129517 28.474118147160965 24.244065925098848 70-71 23.898077895290058 27.152350631533917 26.414255409495052 22.53531606368097 72-73 22.21864098243447 24.205464867026244 31.23527374345122 22.340620407088068 74-75 19.57362420103425 31.112671000642163 27.685274530442904 21.62843026788068 76-77 21.40723649202537 26.07147865042498 27.37036730760934 25.150917549940317 78-79 21.5552053690865 26.511819242671585 29.199687618092447 22.733287770149467 80-81 23.69118579168304 26.443276535385866 27.747434161317496 22.118103511613597 82-83 21.695955433527452 25.387897242031563 30.7182528260194 22.19789449842158 84-85 20.744426742647075 27.252301545748907 29.300671644641785 22.702600066962226 86-87 20.94271837722667 28.289685676650656 28.1567945049478 22.61080144117487 88-89 20.602251820730746 27.573534286187478 26.296814898160655 25.527398994921125 90-91 21.559885611954467 24.614488732903375 27.928631532767444 25.89699412237471 92-93 23.343236335161098 27.52861441585775 28.131874899419124 20.99627434956203 94-95 21.91980255771377 26.43818296453389 28.34861240824969 23.29340206950265 96-97 17.975346434403065 26.674263444104685 31.35185553536491 23.99853458612734 98-99 21.19259792536744 28.37939195028542 26.361647595669847 24.066362528677296 100-101 20.373669978784378 24.816703194567758 28.623380148821703 26.18624667782616 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4914.0 1 3013.5 2 1308.0 3 1464.0 4 1193.0 5 1125.0 6 1872.5 7 3543.0 8 5319.0 9 5867.5 10 4751.0 11 3712.0 12 3706.0 13 3800.5 14 3986.0 15 4498.0 16 5183.0 17 5194.5 18 4690.5 19 4646.5 20 5018.0 21 5482.0 22 6192.5 23 7065.5 24 7970.0 25 9413.0 26 11990.5 27 14852.0 28 16266.0 29 17728.0 30 20469.0 31 23106.0 32 26464.5 33 30653.0 34 35605.5 35 39031.0 36 41984.5 37 46328.5 38 47442.5 39 45790.0 40 44887.5 41 45982.0 42 47008.5 43 48504.0 44 51272.5 45 54636.0 46 69774.0 47 142085.0 48 298780.0 49 293956.0 50 140013.0 51 72056.0 52 51371.5 53 45542.5 54 42549.5 55 38928.0 56 34969.0 57 31018.0 58 27859.0 59 24835.0 60 21876.5 61 18740.5 62 15378.5 63 12067.0 64 8972.5 65 6586.5 66 4464.5 67 3358.0 68 2423.5 69 1440.0 70 1011.5 71 749.5 72 566.0 73 459.0 74 371.0 75 264.0 76 166.5 77 106.0 78 62.5 79 38.5 80 35.5 81 26.5 82 18.5 83 17.5 84 13.0 85 7.0 86 5.5 87 4.0 88 2.5 89 2.5 90 3.0 91 3.5 92 3.0 93 2.5 94 1.0 95 1.0 96 2.0 97 1.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30342707528596985 2 0.08230957076900847 3 0.09170359786764531 4 0.09640061141696374 5 0.10619723967697071 6 0.05318808676323428 7 0.05502215872058719 8 0.05609576181757425 9 0.05913763725903761 10-11 0.05618522874232318 12-13 0.05511162564533611 14-15 0.0533446538815449 16-17 0.054440623709719194 18-19 0.06155324422725851 20-21 0.06320838233511357 22-23 0.07788095799393682 24-25 0.08620138199558659 26-27 0.08132543459677033 28-29 0.07899929455329835 30-31 0.07325104463818011 32-33 0.0651990214107771 34-35 0.3025771395008551 36-37 0.4729445309539903 38-39 0.44733462374461125 40-41 0.42897153743989497 42-43 0.458562722800601 44-45 0.4682475174046719 46-47 0.4629689688444854 48-49 0.4788940814497936 50-51 0.4761429735137643 52-53 0.47518120407271336 54-55 0.466704212952753 56-57 0.4461939204540626 58-59 0.4297543730314481 60-61 0.39121649519584983 62-63 0.45719835219817995 64-65 0.4803479189769636 66-67 0.4897866795379749 68-69 0.501529213411271 70-71 0.4990688729806756 72-73 0.4879302408494348 74-75 0.48967484588203875 76-77 0.465541142931017 78-79 0.446529421421871 80-81 0.45263553903598497 82-83 0.4604191614891412 84-85 0.46200719940343454 86-87 0.4622756001776813 88-89 0.468068583555174 90-91 0.4811083878373294 92-93 0.4869237379460094 94-95 0.4927614547858766 96-97 0.4847094315584736 98-99 0.4886907097098006 100-101 0.46753178200668055 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2235463.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.82196277244614 #Duplication Level Percentage of deduplicated Percentage of total 1 72.08466187156417 23.65960088412847 2 14.00809421818106 9.195462938841136 3 5.456678333224104 5.37296679342885 4 2.6512347928389888 3.4807491868630103 5 1.4875031808510373 2.441138701289398 6 0.9096805741627719 1.791450116399276 7 0.6196285036718745 1.423619657618534 8 0.4429152427011823 1.1629878085829723 9 0.3293965961197615 0.9730298533691962 >10 1.747762548465596 10.66181683689318 >50 0.16153482902742697 3.6748447912652695 >100 0.08518519785327187 5.21020350241057 >500 0.006152913132607555 1.356166171911587 >1k 0.005332524714926547 4.174634820594979 >5k 0.00150404543241518 3.698390225594291 >10k+ 0.002734628058936691 21.72293771080923 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 91864 4.1093947875674965 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 52870 2.36505815573776 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 39095 1.7488547115295578 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 34214 1.530510681679813 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 25548 1.142850496742733 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 22884 1.0236805529771684 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 22260 0.9957668724555048 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 21460 0.9599801025559358 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 19871 0.8888986308429171 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 19138 0.8561090029224372 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 19137 0.8560642694600625 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 16623 0.7436043450506673 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 14713 0.6581634319154466 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 13822 0.6183059169398017 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 12642 0.5655204313379376 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 12109 0.5416774958923498 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11652 0.5212343035872211 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 11431 0.5113482084024652 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 11395 0.5097378037569845 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 11317 0.5062485936917765 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9684 0.43319884963428157 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 9613 0.4300227738056948 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 9122 0.4080586437798344 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 8442 0.3776398893652009 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 7872 0.35214181581175796 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 7532 0.3369324386044412 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 6931 0.3100476277173901 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6121 0.2738135231940766 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 5851 0.2617354883529721 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 5804 0.25963301562137237 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 5438 0.24326056839231963 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 4922 0.22017810180709768 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 4477 0.2002717110504625 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 4123 0.18443606536990323 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 4004 0.17911278334734235 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3981 0.17808391371272975 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 3980 0.17803918025035528 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 3311 0.1481124939218408 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 3306 0.1478888266099685 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 3241 0.14498115155562852 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 3226 0.1443101496200116 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3143 0.14059727224293134 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3068 0.13724226256484673 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 3028 0.13545292406986828 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 2889 0.12923497279981822 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 2631 0.11769373950720724 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 2555 0.1142939963667482 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 2446 0.10941804896793192 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 2274 0.10172389343952461 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.2366731187230563E-5 0.0 14-15 0.0 0.0 0.0 2.460340430595362E-4 0.0 16-17 0.0 0.0 0.0 5.591682796807641E-4 0.0 18-19 0.0 0.0 0.0 7.604688603658392E-4 0.0 20-21 0.0 0.0 0.0 0.0010959698281742976 0.0 22-23 0.0 0.0 0.0 0.001744605032603984 0.0 24-25 0.0 0.0 0.0 0.00239324023703367 0.0 26-27 0.0 0.0 0.0 0.00357867698995689 0.0 28-29 0.0 0.0 0.0 0.008186223614526388 0.0 30-31 0.0 0.0 0.0 0.019615623251201204 0.0 32-33 0.0 0.0 0.0 0.046657001256562955 0.0 34-35 0.0 0.0 0.0 0.08877355608211811 0.0 36-37 0.0 0.0 0.0 0.1454732196417476 0.0 38-39 0.0 0.0 0.0 0.22867745965824526 0.0 40-41 0.0 0.0 0.0 0.3163326791810019 0.0 42-43 0.0 0.0 0.0 0.41145838692029346 0.0 44-45 0.0 0.0 0.0 0.5310980320407898 0.0 46-47 0.0 0.0 0.0 0.6876651503514037 0.0 48-49 0.0 0.0 0.0 0.8327805022941557 0.0 50-51 0.0 0.0 0.0 0.9997481506068319 0.0 52-53 0.0 0.0 0.0 1.1611241161227004 0.0 54-55 0.0 0.0 0.0 1.3441063439654335 0.0 56-57 0.0 0.0 0.0 1.6201118068158586 0.0 58-59 0.0 0.0 0.0 1.8790067203080525 0.0 60-61 0.0 0.0 0.0 2.1779828160877637 0.0 62-63 0.0 0.0 0.0 2.472172431393407 0.0 64-65 0.0 0.0 0.0 2.7490949302225087 0.0 66-67 0.0 0.0 0.0 3.099492141001663 0.0 68-69 0.0 0.0 0.0 3.5110623615778924 0.0 70-71 0.0 0.0 0.0 3.9376182920495664 0.0 72-73 0.0 0.0 0.0 4.348360943571869 0.0 74-75 0.0 0.0 0.0 4.827255025021662 0.0 76-77 0.0 0.0 0.0 5.252401851428541 0.0 78-79 0.0 0.0 0.0 5.895803240760415 0.0 80-81 0.0 0.0 0.0 6.582282954358895 0.0 82-83 0.0 0.0 0.0 7.13959479535112 0.0 84-85 0.0 0.0 0.0 7.797020125137388 0.0 86-87 0.0 0.0 0.0 8.460193704838774 0.0 88-89 0.0 0.0 0.0 9.171209722549646 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 750 0.0 38.364525 94-95 TAGGACG 125 5.808033E-9 34.107758 4 TATAACG 60 0.0040043336 31.581255 2 TTTGCGT 1820 0.0 30.71364 3 CTTTGCG 1875 0.0 29.812706 2 TTGCGTT 1975 0.0 28.543058 4 GCTTTGC 2170 0.0 26.031761 1 GTACATG 10495 0.0 22.524918 1 GTCGGGA 275 5.4569682E-11 22.393982 2 TACATGG 10615 0.0 21.912008 2 GCTTATG 545 0.0 21.775084 1 GAGTACT 8020 0.0 21.755297 12-13 TACGCCG 155 3.9907936E-5 21.393755 5 CATGGGG 2550 0.0 21.36379 4 ACATGGG 10725 0.0 21.245573 3 AGTACTT 8165 0.0 20.26716 12-13 ATGGGAG 2405 0.0 19.69725 5 GTACTTT 8950 0.0 19.389751 14-15 CATGGGA 7275 0.0 19.33945 4 GTGTACG 200 1.2927156E-5 18.987873 1 >>END_MODULE