##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938971_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2235463 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.03101728814121 33.0 31.0 34.0 30.0 34.0 2 32.24627247241399 34.0 31.0 34.0 30.0 34.0 3 32.347294050494234 34.0 31.0 34.0 30.0 34.0 4 35.8608010957909 37.0 35.0 37.0 35.0 37.0 5 35.81135093714367 37.0 35.0 37.0 35.0 37.0 6 35.90950375828184 37.0 35.0 37.0 35.0 37.0 7 35.857035880262835 37.0 35.0 37.0 35.0 37.0 8 35.8585196892098 37.0 35.0 37.0 35.0 37.0 9 37.44767817673565 39.0 37.0 39.0 34.0 39.0 10-11 37.33958200158088 39.0 37.0 39.0 34.0 39.0 12-13 37.27656574946667 39.0 37.0 39.0 34.0 39.0 14-15 38.342706410260426 40.0 37.5 41.0 33.5 41.0 16-17 38.56267694880211 40.0 38.0 41.0 34.0 41.0 18-19 38.69051914525089 40.0 38.0 41.0 34.0 41.0 20-21 38.657356216586905 40.0 38.0 41.0 34.0 41.0 22-23 38.592931531409825 40.0 38.0 41.0 34.0 41.0 24-25 38.436646457579485 40.0 38.0 41.0 34.0 41.0 26-27 38.173273948170916 40.0 37.5 41.0 33.5 41.0 28-29 38.06290083978129 40.0 37.0 41.0 33.0 41.0 30-31 37.96205685354667 40.0 37.0 41.0 33.0 41.0 32-33 37.83196791895013 40.0 37.0 41.0 33.0 41.0 34-35 37.62821616819424 40.0 37.0 41.0 33.0 41.0 36-37 37.42600347221135 40.0 36.5 41.0 32.0 41.0 38-39 37.237929905348466 39.0 36.0 41.0 31.0 41.0 40-41 37.20611166456345 40.0 36.0 41.0 31.0 41.0 42-43 36.99578633151164 39.5 36.0 41.0 31.0 41.0 44-45 36.742477732800765 39.0 36.0 41.0 30.5 41.0 46-47 36.38812451827653 39.0 35.0 41.0 30.0 41.0 48-49 35.96584756714829 39.0 35.0 40.0 28.5 41.0 50-51 35.66425926083322 39.0 35.0 40.0 28.0 41.0 52-53 35.46967205451399 38.5 35.0 40.0 28.0 41.0 54-55 35.24394991104751 38.0 34.5 40.0 27.0 41.0 56-57 35.047965231363705 38.0 34.0 40.0 26.5 41.0 58-59 34.76052992154198 38.0 34.0 40.0 26.0 41.0 60-61 34.18221996964387 37.5 33.0 40.0 25.0 41.0 62-63 33.821703378673675 37.0 33.0 39.5 24.0 41.0 64-65 33.38922854012793 36.5 32.5 39.0 22.5 40.5 66-67 32.81142832603358 36.0 32.0 39.0 22.0 40.0 68-69 32.24745321215336 35.0 31.0 38.0 21.5 40.0 70-71 31.549230070012342 35.0 30.5 37.0 19.0 39.0 72-73 30.917137747303354 34.0 29.5 36.5 16.0 39.0 74-75 30.403414192048807 34.0 29.5 36.0 12.5 38.0 76-77 28.535162514432134 31.5 27.0 34.0 8.0 36.0 78-79 29.517331979996985 34.0 29.0 35.0 7.5 37.0 80-81 29.43464329313435 34.0 29.0 35.0 6.5 36.5 82-83 28.971375281093895 33.5 29.0 35.0 2.0 36.0 84-85 28.45260042326802 33.0 28.5 35.0 2.0 35.5 86-87 27.85564936659654 33.0 26.5 35.0 2.0 35.0 88-89 27.030985527382917 32.0 25.5 34.0 2.0 35.0 90-91 26.31537113340726 32.0 24.5 34.0 2.0 35.0 92-93 25.906774569742375 32.0 24.0 34.0 2.0 35.0 94-95 25.246791827912162 31.0 21.5 34.0 2.0 35.0 96-97 24.12075104799319 31.0 12.5 34.0 2.0 35.0 98-99 22.82776028947918 31.0 2.0 34.0 2.0 35.0 100-101 17.333849632044906 23.5 2.0 30.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2127242666645799 1101 2 0.2085260718899704 1101 3 0.25845610657950147 1101 4 0.14604531111849894 1101 5 0.13036537462641462 1101 6 0.16856266960215294 1101 7 0.20170232773511998 1101 8 0.23436182286855 1101 9 0.27649919916274257 1101 10-11 0.26157300762374547 1101 12-13 0.3279566223816843 1101 14-15 0.3827469536524575 1101 16-17 0.3646504858562736 1101 18-19 0.3441210935555077 1101 20-21 0.35390833080617057 1101 22-23 0.4611099417326301 1101 24-25 0.4862624511186553 1101 26-27 0.33690463933906045 1101 28-29 0.40988332715866704 1101 30-31 0.37330169651554 1101 32-33 0.3260442350343027 1101 34-35 0.5344271909874081 1101 36-37 0.6390953082456079 1101 38-39 0.6282680263669462 1101 40-41 0.4871320220558175 1101 42-43 0.5153461673227113 1101 44-45 0.5384986138330632 1101 46-47 0.5729179745954625 1101 48-49 0.5863519890420292 1101 50-51 0.5656859183611758 1101 52-53 0.47710667998699563 1101 54-55 0.5484761241340408 1101 56-57 0.5300112790186873 1101 58-59 0.643876521466737 1101 60-61 0.6679636720294155 1101 62-63 0.6477979322905796 1101 64-65 0.499245602383219 1101 66-67 0.4423338169039397 1101 68-69 0.5951422141418377 1101 70-71 0.7875473552069572 1101 72-73 0.8715308483791766 1101 74-75 0.827642552467438 1101 76-77 0.5364979034800577 1101 78-79 0.8230070179298572 1101 80-81 0.9261835941025574 1101 82-83 0.9486752437399062 1101 84-85 1.0658137127599723 1101 86-87 1.137515314799817 1101 88-89 1.0485614924769422 1101 90-91 1.172186699556665 1101 92-93 1.1300079271201646 1101 94-95 1.0161929580329065 1101 96-97 1.109445861340621 1101 98-99 1.5188001274911969 1101 100-101 1.6830835376820303 1102 1 0.14449570320375926 1102 2 0.11680610062583696 1102 3 0.21128698816949765 1102 4 0.05537040764503587 1102 5 0.06955488048562586 1102 6 0.1393868111810903 1102 7 0.16823520924723567 1102 8 0.059881203140086825 1102 9 0.13696632721059387 1102 10-11 0.19736406121857186 1102 12-13 0.22437716027214805 1102 14-15 0.31481661457902277 1102 16-17 0.25786027330552486 1102 18-19 0.17336967768917333 1102 20-21 0.21936995954036576 1102 22-23 0.2730665245521564 1102 24-25 0.3390224956727721 1102 26-27 0.37771929323205455 1102 28-29 0.3016187809085906 1102 30-31 0.21341087135734682 1102 32-33 0.36060946748027334 1102 34-35 0.4906293605406873 1102 36-37 0.2771053471453513 1102 38-39 0.3786552633891205 1102 40-41 0.3971566284823993 1102 42-43 0.42323493685448454 1102 44-45 0.3135349475431184 1102 46-47 0.50554397906253 1102 48-49 0.4367467574541237 1102 50-51 0.4746982173774015 1102 52-53 0.535960994692303 1102 54-55 0.46084303946093996 1102 56-57 0.5186140476322407 1102 58-59 0.4438585440623015 1102 60-61 0.5578525362281965 1102 62-63 0.5483428971367061 1102 64-65 0.5476908600208787 1102 66-67 0.4656362404492498 1102 68-69 0.36113330191937365 1102 70-71 0.5771288314386602 1102 72-73 0.6802436872071986 1102 74-75 0.7796533752846138 1102 76-77 0.5239125545283763 1102 78-79 0.8706003890942426 1102 80-81 0.9534573685204073 1102 82-83 0.9852662568564163 1102 84-85 1.0381455313434813 1102 86-87 1.1570575228774267 1102 88-89 1.020930618255118 1102 90-91 1.0631868518166492 1102 92-93 1.1421676242976666 1102 94-95 1.3863213670652605 1102 96-97 1.2804799957915556 1102 98-99 1.539313488480044 1102 100-101 1.5205289788244514 1103 1 0.049061058497770205 1103 2 0.06685768896129929 1103 3 0.07695908081516478 1103 4 0.07995345306542845 1103 5 0.08420233344725858 1103 6 0.06301719515311532 1103 7 0.06245410615363767 1103 8 0.10385447726719121 1103 9 0.11924622956563269 1103 10-11 0.1813414699643232 1103 12-13 0.05471136112021924 1103 14-15 0.1908525111871242 1103 16-17 0.09984633454319436 1103 18-19 0.1705999315811937 1103 20-21 0.13590954332208582 1103 22-23 0.11277972550217896 1103 24-25 0.09879218419816738 1103 26-27 0.20555655728503552 1103 28-29 0.1777198119201202 1103 30-31 0.273012390404908 1103 32-33 0.3570460728262077 1103 34-35 0.29051806566874205 1103 36-37 0.2487443883291789 1103 38-39 0.18798385512667437 1103 40-41 0.3765459556912063 1103 42-43 0.47455954878559936 1103 44-45 0.41233097585487855 1103 46-47 0.411231250856126 1103 48-49 0.30720162686436225 1103 50-51 0.39695417103947506 1103 52-53 0.4732468299419068 1103 54-55 0.5710285025948636 1103 56-57 0.5025637270576979 1103 58-59 0.4200470817288675 1103 60-61 0.4132808279699134 1103 62-63 0.4839032606810747 1103 64-65 0.487731455745795 1103 66-67 0.4591363697740789 1103 68-69 0.45193986406892606 1103 70-71 0.6222772387609048 1103 72-73 0.45471192144183803 1103 74-75 0.5784090721849715 1103 76-77 0.40674791646798525 1103 78-79 0.5924707046090667 1103 80-81 0.6347904856830411 1103 82-83 0.6100330945716905 1103 84-85 0.527308794973564 1103 86-87 0.9598398710765075 1103 88-89 0.7006777362937626 1103 90-91 0.5109448123735234 1103 92-93 0.6553981679905796 1103 94-95 0.8784727894495923 1103 96-97 0.47283581276576925 1103 98-99 0.9910875985930581 1103 100-101 1.1356215876744216 1104 1 0.031646778457172786 1104 2 0.1237000245452009 1104 3 0.08023195785795423 1104 4 0.1164221290579519 1104 5 0.10995417581961675 1104 6 0.1511826188619878 1104 7 0.1380355015024719 1104 8 0.08967446167505955 1104 9 0.13560417934719027 1104 10-11 0.226845493028911 1104 12-13 0.19949525930064738 1104 14-15 0.32344586173064727 1104 16-17 0.20036319169865635 1104 18-19 0.207248340006835 1104 20-21 0.3665182065172914 1104 22-23 0.2857990832827966 1104 24-25 0.3301625267838162 1104 26-27 0.22154309068309175 1104 28-29 0.24201851527242724 1104 30-31 0.15534050919931275 1104 32-33 0.27271846518900134 1104 34-35 0.3463463609778614 1104 36-37 0.26349761707754027 1104 38-39 0.15107986810050988 1104 40-41 0.2518994447987879 1104 42-43 0.22763246775063095 1104 44-45 0.22841872941091168 1104 46-47 0.3445822356611572 1104 48-49 0.292341753807861 1104 50-51 0.02317138710819222 1104 52-53 0.08211366062016623 1104 54-55 0.3475195003443048 1104 56-57 0.33211532451588965 1104 58-59 0.3301325994318205 1104 60-61 0.4276437937113542 1104 62-63 0.35858148906169873 1104 64-65 0.5129275525641219 1104 66-67 0.4969082208250839 1104 68-69 0.3429282361619457 1104 70-71 0.4388136338891009 1104 72-73 0.4193386479764136 1104 74-75 0.4647381912294364 1104 76-77 0.3428693549158339 1104 78-79 0.5941305864913069 1104 80-81 0.6391109591837711 1104 82-83 0.6105479291626921 1104 84-85 0.6999884538588717 1104 86-87 0.6493316374004436 1104 88-89 0.6414193555220962 1104 90-91 0.6807685183000025 1104 92-93 0.7961236067032011 1104 94-95 1.065639525986672 1104 96-97 1.0559126481510823 1104 98-99 1.1561582780276218 1104 100-101 1.2229747678812402 1105 1 -0.07458552937870877 1105 2 -0.002404833050931643 1105 3 0.019431349936311904 1105 4 0.04436897146493379 1105 5 -0.044175069744291307 1105 6 -0.07357485684114806 1105 7 0.0359277731652341 1105 8 -0.035354534587646924 1105 9 -0.04242865197677048 1105 10-11 0.10403676615371182 1105 12-13 0.038935509223989584 1105 14-15 0.059135261763529456 1105 16-17 0.03390697938824161 1105 18-19 0.0272647601126792 1105 20-21 -0.010734725327047556 1105 22-23 0.03241418622508263 1105 24-25 -0.10892299108277115 1105 26-27 -0.08705391236984639 1105 28-29 0.04587457382488225 1105 30-31 0.11140694952477759 1105 32-33 0.19454187461623462 1105 34-35 0.20636551406479953 1105 36-37 0.26309489120664864 1105 38-39 0.13930016415124413 1105 40-41 0.2955251552627658 1105 42-43 0.33645988555011996 1105 44-45 0.31138592475017646 1105 46-47 0.23516967105836528 1105 48-49 0.39722134710472545 1105 50-51 0.390558368341722 1105 52-53 0.4398040027538528 1105 54-55 0.46706698089124643 1105 56-57 0.3516783586137109 1105 58-59 0.4360219514708916 1105 60-61 0.4416518771139337 1105 62-63 0.39655326158879944 1105 64-65 0.37336642933111364 1105 66-67 0.4110869489001274 1105 68-69 0.3863123111034028 1105 70-71 0.5602575366749569 1105 72-73 0.514359067566776 1105 74-75 0.4779418227613732 1105 76-77 0.27160765086879834 1105 78-79 0.2207220480362615 1105 80-81 0.306970990557506 1105 82-83 0.3607415708710917 1105 84-85 0.4666064230983906 1105 86-87 0.6501938596029149 1105 88-89 0.7434275411337268 1105 90-91 0.5261904416965457 1105 92-93 0.8223142310227303 1105 94-95 0.9716159779106412 1105 96-97 0.9909691297601206 1105 98-99 1.1063718060617838 1105 100-101 0.962010514385021 1106 1 0.06370994130670482 1106 2 0.08256106333430324 1106 3 0.060543212142114555 1106 4 0.11939713751595349 1106 5 0.0808108605790423 1106 6 0.04910930641622713 1106 7 0.13301954163014784 1106 8 0.1586880385987044 1106 9 0.17906725099745557 1106 10-11 0.22802409650331157 1106 12-13 0.1881615435187598 1106 14-15 0.1156843625323134 1106 16-17 -0.01611923773223367 1106 18-19 0.058838075676042934 1106 20-21 0.1887053709554678 1106 22-23 0.23903371547025642 1106 24-25 0.25445793887212886 1106 26-27 0.20205108532881155 1106 28-29 0.1683291370455109 1106 30-31 0.18480559605925606 1106 32-33 0.24479972079701895 1106 34-35 0.11946243411379243 1106 36-37 0.143822477704461 1106 38-39 0.18865359976872043 1106 40-41 0.14038259499809413 1106 42-43 0.29328674896799356 1106 44-45 0.18525904243033864 1106 46-47 0.28742824128020317 1106 48-49 0.3017154207540145 1106 50-51 0.3661744172774988 1106 52-53 0.3250052658744167 1106 54-55 0.43859006210239215 1106 56-57 0.3629575939067635 1106 58-59 0.31655015970424216 1106 60-61 0.40287841118924916 1106 62-63 0.4218877568051056 1106 64-65 0.3133556007526366 1106 66-67 0.3635053848219627 1106 68-69 0.17848455171142064 1106 70-71 0.44849987897978494 1106 72-73 0.5402254873333128 1106 74-75 0.4606195145014311 1106 76-77 0.13766731911276509 1106 78-79 0.4077494323792479 1106 80-81 0.5192666598736473 1106 82-83 0.630837928133019 1106 84-85 0.7916014306324755 1106 86-87 1.0193046453583143 1106 88-89 0.9777240199234924 1106 90-91 0.9286944703762607 1106 92-93 1.0964891392628076 1106 94-95 1.175882160174627 1106 96-97 1.333443427904843 1106 98-99 1.4049042880595373 1106 100-101 1.6863939314956724 1107 1 -0.007463489305848725 1107 2 0.02654390491419889 1107 3 -0.02153924595231871 1107 4 0.06794430323698464 1107 5 0.001258185525031763 1107 6 -0.011865254967609928 1107 7 -0.016336083196982543 1107 8 -0.06812840916479956 1107 9 -0.13054991140950278 1107 10-11 0.07741043256016411 1107 12-13 -3.371943929764143E-4 1107 14-15 0.047419154555619514 1107 16-17 0.16139852245355968 1107 18-19 -0.004370680948987626 1107 20-21 0.08689733296075275 1107 22-23 -0.0285342915266682 1107 24-25 -0.06414677458524665 1107 26-27 -0.08993577549761511 1107 28-29 -0.026002865675515352 1107 30-31 0.09557296565801465 1107 32-33 0.13150518453036142 1107 34-35 0.0503350690999298 1107 36-37 0.04487344962455353 1107 38-39 -0.02211669767545743 1107 40-41 -0.14930310390975166 1107 42-43 -0.045279640861544124 1107 44-45 0.06098259401897366 1107 46-47 -0.04368148689115259 1107 48-49 0.019417547989455386 1107 50-51 -0.03214524862418244 1107 52-53 -0.010143954571155689 1107 54-55 -0.01707301390447924 1107 56-57 0.061117598790659144 1107 58-59 -0.05154635454003653 1107 60-61 -0.08246990274555799 1107 62-63 -0.035465994200954754 1107 64-65 0.15091001507142465 1107 66-67 0.00711869493187578 1107 68-69 0.1890120248686813 1107 70-71 0.31457057336504235 1107 72-73 0.13244389712701832 1107 74-75 0.21372116181940015 1107 76-77 0.0768444450400203 1107 78-79 0.375028579391973 1107 80-81 0.2437716671122807 1107 82-83 0.1017251774284702 1107 84-85 0.4112004902334405 1107 86-87 0.2730666152625574 1107 88-89 0.5543890295542369 1107 90-91 0.6561930438302959 1107 92-93 0.5631612255582503 1107 94-95 0.7016250853787689 1107 96-97 0.9600297594764875 1107 98-99 0.8300707391869473 1107 100-101 0.6153217295814848 1108 1 0.02054870009874321 1108 2 0.1258210716846193 1108 3 0.08073147257695723 1108 4 0.06070444250055118 1108 5 0.028749673693006628 1108 6 0.11287055160052972 1108 7 0.12303315738620313 1108 8 0.1839005963919078 1108 9 0.19237516141181032 1108 10-11 0.17010197760178158 1108 12-13 0.23662125710687576 1108 14-15 0.23251883713921728 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-1.137553409893286 2316 60-61 -1.0058377049802303 2316 62-63 -0.8561300038898096 2316 64-65 -0.8171820842862942 2316 66-67 -0.847159398396542 2316 68-69 -0.7369618320715645 2316 70-71 -0.7844917855175346 2316 72-73 -0.6469523723325139 2316 74-75 -0.825899086450594 2316 76-77 -0.8983863833598136 2316 78-79 -0.8848929679523607 2316 80-81 -1.029850957799514 2316 82-83 -1.1167059489837392 2316 84-85 -1.3301330875308004 2316 86-87 -1.4563369733146487 2316 88-89 -1.5529076819523837 2316 90-91 -1.7784503425183082 2316 92-93 -1.7431246881783515 2316 94-95 -1.5924396280501547 2316 96-97 -1.5886320158100276 2316 98-99 -1.5563467391422492 2316 100-101 -0.6826580213199414 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 6.0 4 15.0 5 64.0 6 147.0 7 370.0 8 733.0 9 1372.0 10 2306.0 11 3650.0 12 5548.0 13 7527.0 14 9199.0 15 12356.0 16 16150.0 17 22592.0 18 22518.0 19 16141.0 20 15748.0 21 17042.0 22 18828.0 23 20702.0 24 23464.0 25 27406.0 26 32435.0 27 39251.0 28 47876.0 29 57465.0 30 69503.0 31 83703.0 32 103237.0 33 130860.0 34 176967.0 35 249297.0 36 357458.0 37 429659.0 38 206855.0 39 7011.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.24540256876055 19.152872951147216 14.760854553340438 18.8408699267518 2 14.743836064385768 25.303482992113942 35.13527175354725 24.817409189953043 3 26.38366190807005 24.451131600030955 30.79438129819192 18.370825193707073 4 11.973134871836393 16.315009463363968 41.2517227974697 30.460132867329943 5 11.26536203014767 41.30799749313677 34.23657649444433 13.190063982271234 6 27.423670174813896 38.30034315038988 16.076937976607084 18.199048698189145 7 25.206277178374233 35.357239193849324 21.154096489183672 18.282387138592764 8 24.35772813059308 28.462962706159757 19.82591525782355 27.353393905423616 9 22.26509676071579 21.87135282489578 24.58573458831571 31.277815826072718 10-11 25.60793893703452 24.45674564955895 26.756425850036436 23.178889563370095 12-13 31.259161972262568 23.329797898690337 25.328869232011446 20.08217089703565 14-15 23.886507557335364 25.92104745187086 30.34770721163223 19.844737779161544 16-17 19.53967301762405 28.942328726126874 29.851557498822363 21.66644075742671 18-19 18.859405736856996 32.34232813641055 28.86192200030642 19.93634412642602 20-21 22.593082506845338 26.09184316627025 26.74233480938848 24.57273951749593 22-23 20.31937008127623 26.662463212318883 28.572067620891062 24.44609908551383 24-25 24.706089705881695 26.630659179471262 27.769193915706875 20.894057198940175 26-27 23.435525437012377 23.297746372899038 33.305113974152114 19.961614215936475 28-29 19.29705389890148 30.967924765473644 29.662356299343806 20.072665036281077 30-31 20.96780398512523 26.683823440602684 28.38159253810061 23.96678003617148 32-33 21.613688081618886 27.17656252865737 29.664615339193716 21.545134050530024 34-35 24.786487491910584 25.208244460664908 28.718822051174104 21.286445996250404 36-37 23.076177918055034 24.198110105018525 32.5287849019593 20.196927074967146 38-39 21.733894112578476 27.00052746415011 31.397202874165124 19.868375549106286 40-41 20.99013993680129 28.385511672658698 29.65064897562128 20.973699414918734 42-43 19.724992165672155 29.120504152697034 26.702028693882784 24.45247498774803 44-45 22.153351462606473 25.361127874003493 27.519977167673108 24.965543495716926 46-47 24.60124731624363 28.09525822550778 27.462832252897023 19.840662205351563 48-49 23.391009079137756 26.793063163877417 28.420087774019816 21.395839982965015 50-51 18.76805145092678 28.210801357489242 32.30228090490038 20.7188662866836 52-53 21.737420838546644 30.467267854578672 26.354115456171716 21.44119585070296 54-55 19.30756626255948 26.428015136014327 28.94145418644818 25.322964414978017 56-57 23.276095377109797 25.979159574548987 27.520965455478354 23.223779592862865 58-59 26.600131204037485 25.536385411524616 26.468435095491984 21.395048288945915 60-61 22.035012487524778 26.981217492286724 31.441520673194567 19.542249346993934 62-63 21.05084221844021 27.046273533725305 30.149002286131715 21.753881961702763 64-65 20.041548709352956 31.112078851290914 27.926275197124873 20.920097242231257 66-67 21.747453030819514 26.693744570327844 26.338059105252658 25.22074329359999 68-69 19.809726137425827 27.853308308835583 28.100153461815385 24.236812091923202 70-71 23.901245513792894 27.52635583769447 26.22998009808259 22.342418550430047 72-73 22.34358162045178 24.40948474656033 30.896328858943317 22.350604774044573 74-75 19.565544140072998 31.50423424588105 27.213847869546488 21.716373744499464 76-77 21.549531487639236 26.214575034731563 27.024694668911813 25.211198808717388 78-79 21.552202724266134 26.863398841634417 28.816323408298594 22.768075025800854 80-81 23.636918332413686 26.66757177554403 27.521177422886623 22.174332469155654 82-83 21.671461348275503 25.742698492437583 30.33168073012168 22.25415942916523 84-85 20.544120794852244 27.587437839960867 29.044408990634814 22.82403237455208 86-87 20.88760857753882 28.42661321356831 27.639815722920847 23.045962485972034 88-89 20.43734075546148 27.90411277657789 25.98531715941883 25.673229308541796 90-91 21.590920060677206 24.754584857952526 27.618724983954035 26.035770097416233 92-93 23.076874902425136 27.95304596855327 27.73315863425161 21.236920494769986 94-95 21.746634055673542 26.83891444311361 27.977212565297478 23.43723893591537 96-97 17.56463873479454 27.22523253572079 30.900466704212953 24.309662025271724 98-99 20.794417084961818 28.700877625798327 25.85643332052465 24.648271968715203 100-101 20.254373498346865 25.23191048973072 28.033221901780088 26.480494110142327 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3535.0 1 2347.0 2 1198.0 3 1242.5 4 1071.0 5 1051.0 6 1721.5 7 3297.0 8 4983.0 9 5553.5 10 4632.5 11 3529.0 12 3356.5 13 3461.5 14 3583.5 15 3665.5 16 3780.0 17 4019.0 18 4148.0 19 4389.0 20 4801.5 21 5297.5 22 6095.5 23 7042.5 24 8068.0 25 9562.0 26 12117.5 27 14789.0 28 16197.0 29 17843.0 30 20524.5 31 23126.5 32 26539.5 33 30887.5 34 35852.0 35 39117.0 36 42325.5 37 46522.5 38 47412.0 39 46218.5 40 45323.0 41 45815.5 42 46966.5 43 49083.5 44 51711.0 45 54700.0 46 67301.0 47 145071.5 48 304420.5 49 292458.0 50 140080.0 51 75881.0 52 53881.0 53 45421.5 54 41680.0 55 38382.5 56 34596.5 57 30703.0 58 27644.0 59 24882.0 60 21817.0 61 18429.5 62 15150.0 63 11863.5 64 8674.0 65 6317.5 66 4246.5 67 3219.0 68 2265.0 69 1222.0 70 856.0 71 643.0 72 485.5 73 386.0 74 295.5 75 215.0 76 129.5 77 63.0 78 26.5 79 8.5 80 5.0 81 2.5 82 1.0 83 1.0 84 0.5 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.36864846342793417 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 8.499357851147614E-4 16-17 0.0023261400434719786 18-19 2.460340430595362E-4 20-21 0.0 22-23 0.0 24-25 8.946692474892225E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.012950337357406496 36-37 0.023440334284217632 38-39 0.01073603096987067 40-41 0.0059271837646161 42-43 0.0048535806676290325 44-45 0.0016104046454806006 46-47 0.0010512363657998365 48-49 0.0028405748607782813 50-51 5.591682796807641E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 6.039017420552252E-4 60-61 0.001431470795982756 62-63 0.001230170215297681 64-65 6.486352044296863E-4 66-67 0.0027287412048421287 68-69 0.00239324023703367 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.08517251236097398 78-79 0.003220809290961201 80-81 2.0130058068507507E-4 82-83 0.0 84-85 1.5656711831061394E-4 86-87 0.08850515530787134 88-89 0.417229003566599 90-91 0.3698339001808574 92-93 0.0 94-95 9.170359786764531E-4 96-97 0.0 98-99 0.0 100-101 4.4733462374461127E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2235463.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.696205083829142 #Duplication Level Percentage of deduplicated Percentage of total 1 71.022912091317 22.511567872971526 2 14.426291695038204 9.145174003301443 3 5.702209957088654 5.422152486928038 4 2.777916015277564 3.521975829035645 5 1.541522086133073 2.443020009166301 6 0.9707821189222797 1.8462065479844876 7 0.6166899560462776 1.368271192398628 8 0.475212482715803 1.2049945848444565 9 0.34473314465304106 0.9834059206904515 >10 1.8436043276625809 10.840204578097062 >50 0.1707213636356743 3.756099627707585 >100 0.09016368205014634 5.302975952316117 >500 0.007066016172070769 1.4566741987423173 >1k 0.005794133261098029 4.419922198237989 >5k 0.0015545235578555692 3.7272852629626985 >10k+ 0.0028264064688283074 22.050069734615256 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 98278 4.3963152152372915 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 48365 2.1635339077408124 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 36360 1.6265086919354066 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 31469 1.4077173274619172 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 26018 1.1638752240587298 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 25409 1.1366325454726829 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 24508 1.0963276958732935 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 22865 1.0228306171920538 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 22063 0.9869543803677359 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 21596 0.9660638534388626 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 18610 0.8324897347887217 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 16298 0.7290659697789674 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 16210 0.7251294250900149 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 14198 0.6351256987925992 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 12896 0.5768827307810507 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 12043 0.5387250873756353 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 11629 0.5202054339526084 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11334 0.5070090625521425 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 11297 0.5053539244442874 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 10818 0.4839265959669205 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9963 0.4456794856367562 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 8720 0.39007579190530106 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 8635 0.3862734476034719 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 8616 0.3854235118183571 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 8260 0.3694983992130489 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 8191 0.3664117903092111 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6869 0.30727415305017347 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 6781 0.3033376083612209 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 6056 0.27090584813973656 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 5765 0.25788841058876844 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 5355 0.23954769101523937 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 4904 0.21937289948435737 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 4613 0.2063554619333892 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 4305 0.19257755552205516 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 4092 0.18304932803629492 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 3947 0.17656297599199808 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 3812 0.1705239585714458 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 3667 0.16403760652714897 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3660 0.16372447229052772 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 3627 0.16224826803217052 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 3481 0.15571718252549918 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 3297 0.14748622544859835 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 3162 0.14144720802804608 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3125 0.13979206992019103 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3070 0.13733172948959566 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 2751 0.12306175499214257 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 2724 0.1218539515080321 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 2571 0.11500973176473957 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 2487 0.11125212092528483 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2272 0.10163442651477568 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.2366731187230563E-5 0.0 14-15 0.0 0.0 0.0 2.460340430595362E-4 0.0 16-17 0.0 0.0 0.0 5.591682796807641E-4 0.0 18-19 4.473346237446113E-5 0.0 0.0 7.604688603658392E-4 0.0 20-21 4.473346237446113E-5 0.0 0.0 0.0011183365593615282 0.0 22-23 4.473346237446113E-5 0.0 0.0 0.001744605032603984 0.0 24-25 4.473346237446113E-5 0.0 0.0 0.00239324023703367 0.0 26-27 4.473346237446113E-5 0.0 0.0 0.00357867698995689 0.0 28-29 4.473346237446113E-5 0.0 0.0 0.008298057270462539 0.0 30-31 4.473346237446113E-5 0.0 0.0 0.019928757487822434 0.0 32-33 4.473346237446113E-5 0.0 0.0 0.04739510338574157 0.0 34-35 4.473346237446113E-5 0.0 0.0 0.09000372629741579 0.0 36-37 4.473346237446113E-5 0.0 0.0 0.14721782467435157 0.0 38-39 4.473346237446113E-5 0.0 0.0 0.23111543335765342 0.0 40-41 4.473346237446113E-5 0.0 0.0 0.31915088731059293 0.0 42-43 4.473346237446113E-5 0.0 0.0 0.41557386545874386 0.0 44-45 4.473346237446113E-5 0.0 0.0 0.5364213140633507 0.0 46-47 8.946692474892225E-5 0.0 0.0 0.6936370675783943 0.0 48-49 8.946692474892225E-5 0.0 0.0 0.8398707560805077 0.0 50-51 8.946692474892225E-5 0.0 0.0 1.0071739053609923 0.0 52-53 8.946692474892225E-5 0.0 0.0 1.1688182716511077 0.0 54-55 8.946692474892225E-5 0.0 0.0 1.3529635695155768 0.0 56-57 8.946692474892225E-5 0.0 0.0 1.630422869893172 0.0 58-59 8.946692474892225E-5 0.0 0.0 1.8900558855145444 0.0 60-61 8.946692474892225E-5 0.0 0.0 2.1903739851654893 4.473346237446113E-5 62-63 8.946692474892225E-5 0.0 0.0 2.485927971073554 4.473346237446113E-5 64-65 8.946692474892225E-5 0.0 0.0 2.765512110913936 4.473346237446113E-5 66-67 8.946692474892225E-5 0.0 0.0 3.1175197263385703 4.473346237446113E-5 68-69 8.946692474892225E-5 2.2366731187230563E-5 0.0 3.5313713534958975 4.473346237446113E-5 70-71 8.946692474892225E-5 4.473346237446113E-5 0.0 3.960253424011044 4.473346237446113E-5 72-73 8.946692474892225E-5 4.473346237446113E-5 0.0 4.3731656484585075 4.473346237446113E-5 74-75 8.946692474892225E-5 4.473346237446113E-5 0.0 4.854318769758211 4.473346237446113E-5 76-77 8.946692474892225E-5 4.473346237446113E-5 0.0 5.281500968703128 4.473346237446113E-5 78-79 8.946692474892225E-5 4.473346237446113E-5 0.0 5.9242537228305725 4.473346237446113E-5 80-81 8.946692474892225E-5 4.473346237446113E-5 0.0 6.610308468536496 4.473346237446113E-5 82-83 8.946692474892225E-5 4.473346237446113E-5 0.0 7.165853337764928 4.473346237446113E-5 84-85 8.946692474892225E-5 4.473346237446113E-5 0.0 7.820885427314163 4.473346237446113E-5 86-87 8.946692474892225E-5 4.473346237446113E-5 0.0 8.482068367939885 4.473346237446113E-5 88-89 8.946692474892225E-5 4.473346237446113E-5 0.0 9.188566305950937 4.473346237446113E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 1470 0.0 43.09581 94-95 CTTTGCG 1490 0.0 32.16668 2 TTTGCGT 1525 0.0 31.428429 3 TTGCGTT 1650 0.0 29.047483 4 GCTTTGC 1900 0.0 25.827791 1 TACGCCG 150 1.0698968E-6 25.308699 5 TATGACG 125 2.2717429E-4 22.77783 4 TTTACGC 150 3.057747E-5 22.145113 3 GAGTACT 9140 0.0 22.067232 12-13 GTACATG 10475 0.0 21.922829 1 CTCCGAC 360 0.0 21.749664 94-95 TACATGG 10490 0.0 21.668613 2 GACCCGT 110 0.0028944998 21.569916 6 CCGTACA 110 0.0028944998 21.569916 9 AGTACTT 9330 0.0 21.38895 12-13 ATAGACC 400 0.0 21.354214 3 TAGACCG 135 3.822521E-4 21.090584 4 TTTTACG 180 5.2161195E-6 21.090584 2 ACATGGG 10655 0.0 20.308718 3 GTACTTT 9955 0.0 20.213175 14-15 >>END_MODULE