##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938970_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2246128 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00120251383714 33.0 31.0 34.0 30.0 34.0 2 32.2107578018706 34.0 31.0 34.0 30.0 34.0 3 32.32004854576409 34.0 31.0 34.0 30.0 34.0 4 35.862500267126364 37.0 35.0 37.0 35.0 37.0 5 35.8008350370059 37.0 35.0 37.0 35.0 37.0 6 35.90026659210873 37.0 35.0 37.0 35.0 37.0 7 35.85190959731592 37.0 35.0 37.0 35.0 37.0 8 35.84878956141413 37.0 35.0 37.0 35.0 37.0 9 37.44549731805133 39.0 37.0 39.0 34.0 39.0 10-11 37.34908696209655 39.0 37.0 39.0 34.0 39.0 12-13 37.304387372402644 39.0 37.0 39.0 34.0 39.0 14-15 38.309586764423045 40.0 37.5 41.0 33.0 41.0 16-17 38.39844723898193 40.0 38.0 41.0 33.5 41.0 18-19 38.629032940242055 40.0 38.0 41.0 34.0 41.0 20-21 38.61957644444128 40.0 38.0 41.0 34.0 41.0 22-23 38.56686929685219 40.0 38.0 41.0 34.0 41.0 24-25 38.430528447176656 40.0 38.0 41.0 34.0 41.0 26-27 38.176735698054614 40.0 37.5 41.0 33.5 41.0 28-29 38.044693356745476 40.0 37.0 41.0 33.0 41.0 30-31 37.974503234009816 40.0 37.0 41.0 33.0 41.0 32-33 37.78757488442332 40.0 37.0 41.0 33.0 41.0 34-35 37.65856976984392 40.0 37.0 41.0 33.0 41.0 36-37 37.48857589594182 40.0 36.5 41.0 32.5 41.0 38-39 37.32069654979591 39.5 36.0 41.0 32.0 41.0 40-41 37.30160369311099 40.0 36.0 41.0 32.0 41.0 42-43 37.11522740467151 40.0 36.0 41.0 31.0 41.0 44-45 36.857935522819716 39.5 36.0 41.0 31.0 41.0 46-47 36.52199607502333 39.0 36.0 41.0 30.0 41.0 48-49 36.15706429019183 39.0 35.0 41.0 29.0 41.0 50-51 35.85664329904618 39.0 35.0 40.0 28.0 41.0 52-53 35.70752112079098 39.0 35.0 40.0 28.0 41.0 54-55 35.531303870482894 38.5 35.0 40.0 28.0 41.0 56-57 35.182962636145405 38.0 34.5 40.0 27.0 41.0 58-59 34.89263835364681 38.0 34.0 40.0 26.0 41.0 60-61 34.24872558465056 37.5 33.0 40.0 25.5 41.0 62-63 34.02273089512263 37.0 33.0 40.0 25.5 41.0 64-65 33.763024413568594 36.5 33.0 39.0 25.5 40.5 66-67 33.19207988146713 36.0 32.5 39.0 22.5 40.0 68-69 32.70503439697114 35.5 32.0 38.5 22.0 40.0 70-71 32.07660605272718 35.0 31.5 37.5 21.5 39.0 72-73 31.46489915089434 35.0 31.0 37.0 20.0 39.0 74-75 31.039049867149156 34.0 31.0 36.5 18.5 38.5 76-77 29.072761881780558 32.5 28.0 34.5 13.5 36.0 78-79 30.086690295477375 34.0 29.5 35.0 14.0 37.0 80-81 30.02937032083657 34.0 30.0 35.0 12.5 37.0 82-83 29.676821178490272 34.0 29.5 35.0 9.0 36.0 84-85 29.248932830185993 34.0 29.0 35.0 4.5 36.0 86-87 28.799895642634795 34.0 29.0 35.0 2.0 35.0 88-89 28.144284297243967 33.0 27.5 35.0 2.0 35.0 90-91 27.57079805781327 33.0 26.0 35.0 2.0 35.0 92-93 27.253274301375523 33.0 26.0 35.0 2.0 35.0 94-95 26.77974563337441 32.5 25.0 34.0 2.0 35.0 96-97 25.965027371547837 32.0 24.5 34.0 2.0 35.0 98-99 25.043668259333394 32.0 20.5 34.0 2.0 35.0 100-101 20.63892062251127 27.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13676208116905286 1101 2 0.23377665241679324 1101 3 0.18296172528074806 1101 4 0.12623756887990822 1101 5 0.21522318459371803 1101 6 0.11223942749982285 1101 7 0.1145180736751712 1101 8 0.22852661418195908 1101 9 0.30825806907967745 1101 10-11 0.2946692445110273 1101 12-13 0.2608957175592437 1101 14-15 0.31982525252045235 1101 16-17 0.3431806228888874 1101 18-19 0.2945426174149688 1101 20-21 0.3077979368064234 1101 22-23 0.21787649170574497 1101 24-25 0.24325525066847575 1101 26-27 0.3183846728949078 1101 28-29 0.4000705908571973 1101 30-31 0.33609256722803593 1101 32-33 0.3599214623647313 1101 34-35 0.4370277129252145 1101 36-37 0.4091147608795609 1101 38-39 0.45185126307535484 1101 40-41 0.4128684952771877 1101 42-43 0.35014492994137214 1101 44-45 0.48131846956142255 1101 46-47 0.459291416620367 1101 48-49 0.293673378457477 1101 50-51 0.31682579452556325 1101 52-53 0.29377170307912337 1101 54-55 0.4843929999991019 1101 56-57 0.3179621899224827 1101 58-59 0.21653082900681397 1101 60-61 0.5922868262599437 1101 62-63 0.47725127225218955 1101 64-65 0.46020400737326383 1101 66-67 0.6389326084243336 1101 68-69 0.6484829876741216 1101 70-71 0.5981082059749951 1101 72-73 0.6631458577828404 1101 74-75 0.618222639280603 1101 76-77 0.33994388107880624 1101 78-79 0.544260557939392 1101 80-81 0.8647275556085994 1101 82-83 0.7012427902271234 1101 84-85 0.739807040200585 1101 86-87 0.8777746249863654 1101 88-89 1.070538256300452 1101 90-91 1.1241442028874928 1101 92-93 1.1133591244236314 1101 94-95 0.9860902000392535 1101 96-97 0.8871139581019776 1101 98-99 1.1061225990461274 1101 100-101 1.642497570269775 1102 1 0.12327476095757106 1102 2 0.06846013309184684 1102 3 0.11994982722857372 1102 4 0.15005924031414963 1102 5 0.1397459647490038 1102 6 0.15646066219353116 1102 7 0.20139090689356465 1102 8 0.15091910860000013 1102 9 0.2118619546368592 1102 10-11 0.19769558796413378 1102 12-13 0.20929506989029534 1102 14-15 0.263179215292503 1102 16-17 0.29781252197744124 1102 18-19 0.118060702586682 1102 20-21 0.26103334821842594 1102 22-23 0.3051537185357489 1102 24-25 0.33192451376356047 1102 26-27 0.2613428024620248 1102 28-29 0.23019365754642251 1102 30-31 0.31955888362568885 1102 32-33 0.24312840985382422 1102 34-35 0.27367563572541087 1102 36-37 0.3485656347601207 1102 38-39 0.4366102008818302 1102 40-41 0.34850405296942455 1102 42-43 0.27742747107183874 1102 44-45 0.23093867285167136 1102 46-47 0.3130252263232123 1102 48-49 0.06622839504982636 1102 50-51 0.1769474938324862 1102 52-53 0.31931458695001425 1102 54-55 0.1403119048696979 1102 56-57 0.08243789468935603 1102 58-59 0.14243971021188173 1102 60-61 -0.11712583168242219 1102 62-63 0.031874200034543776 1102 64-65 0.16559920939087647 1102 66-67 0.2197283518705362 1102 68-69 0.22347865610834816 1102 70-71 0.14752332474892427 1102 72-73 0.22374086768209978 1102 74-75 0.4545847586364111 1102 76-77 0.21623858551992114 1102 78-79 0.1973075034379086 1102 80-81 0.38659597051576 1102 82-83 0.39198223359067796 1102 84-85 0.5354603167932623 1102 86-87 0.6676783900358458 1102 88-89 0.8414348367718141 1102 90-91 0.7820952341653928 1102 92-93 0.7555983852793098 1102 94-95 0.8503084534286636 1102 96-97 0.6415249073871578 1102 98-99 0.6722419247234903 1102 100-101 1.0917251134125117 1103 1 -0.03908015328156722 1103 2 0.06317651967466276 1103 3 0.05855706655286497 1103 4 0.07358887838372397 1103 5 0.09586012229203789 1103 6 0.0027455809687708665 1103 7 0.09712889943361347 1103 8 0.08125020788325799 1103 9 0.0948355763836446 1103 10-11 0.1348303851952295 1103 12-13 0.07993992482018797 1103 14-15 0.1411591043683771 1103 16-17 0.06738987316528267 1103 18-19 0.1000039951130347 1103 20-21 0.1705260255981571 1103 22-23 0.062234771518618004 1103 24-25 0.14903011714049086 1103 26-27 0.15275249074180408 1103 28-29 0.20703160436761436 1103 30-31 0.2329699837320618 1103 32-33 0.25433713204339625 1103 34-35 0.16608961764006835 1103 36-37 0.04646963591964237 1103 38-39 0.14194261983823964 1103 40-41 0.31807451501607176 1103 42-43 0.2235322432783633 1103 44-45 0.3385536566443008 1103 46-47 0.5788113131210153 1103 48-49 0.4567865586373969 1103 50-51 0.48506768876833917 1103 52-53 0.5611982615642432 1103 54-55 0.6214042318873894 1103 56-57 0.5840878216376169 1103 58-59 0.45698168073887757 1103 60-61 0.563736827472404 1103 62-63 0.5438790877460136 1103 64-65 0.519168052936692 1103 66-67 0.471714795472181 1103 68-69 0.5519148836862016 1103 70-71 0.5567919932031913 1103 72-73 0.4627713642102371 1103 74-75 0.5010268527206101 1103 76-77 0.46939215700281167 1103 78-79 0.5996601898898355 1103 80-81 0.7727597343488419 1103 82-83 0.6625857984080987 1103 84-85 0.5471388649917479 1103 86-87 0.48981217178964087 1103 88-89 0.7459319920933183 1103 90-91 0.7272224417101647 1103 92-93 0.8445889784701563 1103 94-95 0.6973017459102842 1103 96-97 0.5802125573042645 1103 98-99 0.9225242005736511 1103 100-101 1.0623085923572368 1104 1 -0.10295207729440037 1104 2 -0.01878349303629534 1104 3 -0.03194388459264985 1104 4 -0.034031168368237275 1104 5 -0.0489414430714703 1104 6 0.0035565576667906385 1104 7 -0.063940518952883 1104 8 0.03449912070435346 1104 9 0.0861342273676513 1104 10-11 -0.031163006846483654 1104 12-13 0.025940216475795808 1104 14-15 -0.04635368277092056 1104 16-17 -0.010243733395661536 1104 18-19 -0.037270026208297224 1104 20-21 -0.0487747192118988 1104 22-23 -0.04242845332173317 1104 24-25 -0.0420693809506858 1104 26-27 0.027914316070571488 1104 28-29 -0.08150175972740215 1104 30-31 -0.1342261611902984 1104 32-33 -0.10025209062913376 1104 34-35 -0.14471556947658826 1104 36-37 -0.022504737564943866 1104 38-39 0.17607437852151975 1104 40-41 0.09580541266236509 1104 42-43 -0.070924776697872 1104 44-45 -0.049137715138236615 1104 46-47 -0.011621186019247887 1104 48-49 -0.08850995063681211 1104 50-51 -0.09340834541195164 1104 52-53 -0.048038841381476516 1104 54-55 0.10321482551537287 1104 56-57 0.09719458500044453 1104 58-59 0.13889979516364548 1104 60-61 0.2549822621916036 1104 62-63 0.14975257451050794 1104 64-65 0.18207794876775552 1104 66-67 0.11812590798594869 1104 68-69 0.1386541305182547 1104 70-71 0.10994992688853955 1104 72-73 0.20151421973624295 1104 74-75 0.2856930594130169 1104 76-77 0.07880349152308597 1104 78-79 0.2658964831647772 1104 80-81 0.29138836535226886 1104 82-83 0.5074910756332791 1104 84-85 0.34201149420171006 1104 86-87 -0.0018506133907720823 1104 88-89 0.12132877430493494 1104 90-91 0.3026673253308729 1104 92-93 0.36638686113754204 1104 94-95 0.493719823333965 1104 96-97 0.3073566612903704 1104 98-99 0.5790951577699239 1104 100-101 1.1564181286248285 1105 1 -0.02725136430305497 1105 2 -0.01450575665680276 1105 3 0.046442400908730974 1105 4 0.015109981901886727 1105 5 0.028384670024877323 1105 6 0.01805538910615212 1105 7 0.03341349564566087 1105 8 0.06677235219790845 1105 9 -0.11970116647115248 1105 10-11 -0.05840084280767144 1105 12-13 -0.10091857821318939 1105 14-15 -0.021446087973551187 1105 16-17 -0.047336113194802465 1105 18-19 0.009129452173333164 1105 20-21 0.016327233469866087 1105 22-23 -0.059461812340828146 1105 24-25 0.0830854745034415 1105 26-27 -0.03631597928736596 1105 28-29 0.06952835187860273 1105 30-31 0.10233761427670629 1105 32-33 -0.032970200778670744 1105 34-35 0.05928600977384235 1105 36-37 0.09196339798809561 1105 38-39 0.20427871151049715 1105 40-41 0.15470584367494666 1105 42-43 0.05938211865667853 1105 44-45 0.14590659278595552 1105 46-47 0.015479788628063318 1105 48-49 0.02570637280044963 1105 50-51 0.1414178311931238 1105 52-53 0.08568736401422683 1105 54-55 0.1454662311752699 1105 56-57 0.2695687106969231 1105 58-59 0.035587920724637456 1105 60-61 0.25793181932405673 1105 62-63 0.13219315150849553 1105 64-65 0.08842681069130265 1105 66-67 -0.018477666285399152 1105 68-69 0.05033571468002407 1105 70-71 0.22166665254776063 1105 72-73 0.30322779112852416 1105 74-75 0.47144974476536206 1105 76-77 0.32153999803484723 1105 78-79 0.3615086649860366 1105 80-81 0.2776427474546601 1105 82-83 0.291885075328441 1105 84-85 0.16652618165408128 1105 86-87 0.3432515933259097 1105 88-89 0.3338664561516005 1105 90-91 0.3057991525193877 1105 92-93 0.5111921645399136 1105 94-95 0.5507779719274559 1105 96-97 0.46555119276529666 1105 98-99 0.4089099663122724 1105 100-101 0.8875994333481856 1106 1 -0.007714303029608516 1106 2 -0.015101949307165796 1106 3 -0.02899346789657642 1106 4 0.02834612140641468 1106 5 0.05229574351126587 1106 6 0.03515363105226754 1106 7 0.08975898172801067 1106 8 0.028078641564434292 1106 9 0.09557863612053552 1106 10-11 0.091858963826283 1106 12-13 0.0965432646288562 1106 14-15 0.1315201930765113 1106 16-17 0.013652282688717321 1106 18-19 0.08503208660348349 1106 20-21 0.057091569428457944 1106 22-23 0.10946292449779094 1106 24-25 0.14464521304842748 1106 26-27 0.12629913828621397 1106 28-29 0.1703020976996612 1106 30-31 0.14677633171907445 1106 32-33 0.041190552971187344 1106 34-35 0.0732271641717972 1106 36-37 0.03373159066496356 1106 38-39 0.03126014608379535 1106 40-41 0.04241713070557296 1106 42-43 0.13165365200038792 1106 44-45 0.16315610903950528 1106 46-47 0.10130781924987531 1106 48-49 -0.08618039159846091 1106 50-51 0.022225998390482005 1106 52-53 0.040607309774983946 1106 54-55 0.08123035590895711 1106 56-57 0.21849742767543745 1106 58-59 0.19587293767233405 1106 60-61 0.3547827873139582 1106 62-63 0.14180233159139988 1106 64-65 0.14446218962049784 1106 66-67 0.07775857510408457 1106 68-69 0.27985580341105276 1106 70-71 0.25210726770095704 1106 72-73 0.17113053075696527 1106 74-75 0.324308404655163 1106 76-77 0.21978157140361176 1106 78-79 0.320366816279396 1106 80-81 0.4135711513233069 1106 82-83 0.493956694653118 1106 84-85 0.5063345592480069 1106 86-87 0.46945723195405975 1106 88-89 0.4946754972402303 1106 90-91 0.6761447495983504 1106 92-93 0.7492779409032693 1106 94-95 0.733042493432638 1106 96-97 0.6654260730711847 1106 98-99 0.63661248543848 1106 100-101 1.1160244659016705 1107 1 -0.18693202953953403 1107 2 -0.12427002477048177 1107 3 -0.17206662853408972 1107 4 -0.134738835536659 1107 5 -0.1672867432705516 1107 6 -0.1710419488852537 1107 7 -0.130546524412388 1107 8 -0.07043723780760303 1107 9 -0.19569717810747989 1107 10-11 -0.0613111693819306 1107 12-13 -0.04625935748830301 1107 14-15 -0.02231843875029682 1107 16-17 -0.06110298320934504 1107 18-19 -0.057965385203715414 1107 20-21 -0.13559505155368612 1107 22-23 -0.03771281856413111 1107 24-25 -0.0771553798233171 1107 26-27 -0.17164782542485568 1107 28-29 -0.11692100818993367 1107 30-31 -0.18183018425281716 1107 32-33 -0.0053104752817105805 1107 34-35 -0.2050545403613171 1107 36-37 -0.02323256649189176 1107 38-39 -0.06908796058024791 1107 40-41 -0.007910262207637686 1107 42-43 -0.008064108171957685 1107 44-45 -0.020732330787566866 1107 46-47 -0.04889795523995133 1107 48-49 0.14383362684028356 1107 50-51 0.1428663527422458 1107 52-53 0.17277611589882014 1107 54-55 -0.0064309397764290566 1107 56-57 0.15992108810704764 1107 58-59 0.23556919932562437 1107 60-61 0.022104873128775182 1107 62-63 0.17779304158773357 1107 64-65 0.05158239174375723 1107 66-67 0.10965744160963453 1107 68-69 0.16346734396477558 1107 70-71 0.11718588702104427 1107 72-73 0.13060964291149801 1107 74-75 0.09389931736952306 1107 76-77 -0.17815157625631528 1107 78-79 0.04482353982248455 1107 80-81 0.1456095620106943 1107 82-83 0.04089172038731803 1107 84-85 -0.060929061333904855 1107 86-87 -0.020645911373115666 1107 88-89 0.22085354793377832 1107 90-91 0.4034800236497418 1107 92-93 0.5177070868647888 1107 94-95 0.5189586504474129 1107 96-97 0.2762446400265759 1107 98-99 0.3389815974567121 1107 100-101 1.0501572164688753 1108 1 -0.018092087393277723 1108 2 -0.04070210970285615 1108 3 -0.04798981105831501 1108 4 -0.045714336263174005 1108 5 0.032914875865635906 1108 6 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2316 70-71 -1.799542297649495 2316 72-73 -1.7401809576858618 2316 74-75 -2.075992417798041 2316 76-77 -1.8821400506269548 2316 78-79 -1.9886619606067697 2316 80-81 -2.032698744290702 2316 82-83 -2.180865175541012 2316 84-85 -2.317233200176098 2316 86-87 -2.202481564055148 2316 88-89 -2.472366962408614 2316 90-91 -2.8112891810201397 2316 92-93 -3.1943591812200474 2316 94-95 -3.2312180210344508 2316 96-97 -3.4877040961125942 2316 98-99 -3.7278623404495193 2316 100-101 -4.233629105285708 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 7.0 4 12.0 5 56.0 6 143.0 7 322.0 8 700.0 9 1392.0 10 2272.0 11 3521.0 12 5179.0 13 7457.0 14 8772.0 15 11632.0 16 15558.0 17 21725.0 18 21187.0 19 14800.0 20 13876.0 21 14599.0 22 15754.0 23 17399.0 24 20418.0 25 24225.0 26 28606.0 27 34800.0 28 42408.0 29 51823.0 30 62732.0 31 75817.0 32 93701.0 33 119624.0 34 161658.0 35 231808.0 36 352491.0 37 471465.0 38 283215.0 39 14968.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.22825163822312 19.240606477797943 14.73846538946352 18.79267649451542 2 14.711583667538092 25.3131611377446 35.10583546440809 24.869419730309225 3 26.408824430308513 24.454661533091613 30.738853707357727 18.397660329242143 4 11.949764216464956 16.31483156792489 41.22320722594616 30.51219698966399 5 11.286346550341678 41.27538097903007 34.22798682706764 13.21028564356061 6 27.443271265039215 38.24047427395055 16.086126881460004 18.23012757955023 7 25.206266072102746 35.3613863501991 21.177065599111003 18.255281978587153 8 24.32216685781042 28.454300022082446 19.83992007579265 27.38361304431448 9 22.24539296068612 21.90681029754315 24.631321100133206 31.21647564163752 10-11 25.60072266584985 24.407558251355223 26.750278701837114 23.24144038095781 12-13 31.29231726776034 23.32015361546626 25.348488599046892 20.039040517726505 14-15 23.871298638216746 25.924213491925336 30.381118552655067 19.82336931720285 16-17 19.54380419175673 28.945961288024662 29.89443807530915 21.615796444909453 18-19 18.929353091186254 32.34900237208209 28.780038359345504 19.941606177386152 20-21 22.634395724553542 26.127807498058882 26.681894353304887 24.55590242408269 22-23 20.29879864371042 26.654847809207666 28.60847645370166 24.43787709338025 24-25 24.69810269049671 26.681627226943434 27.71525042205965 20.905019660500205 26-27 23.434372395517975 23.26548175348867 33.33407535100404 19.966070499989318 28-29 19.360650862283897 30.96083571372602 29.625315209106518 20.05319821488357 30-31 20.979725999586847 26.666734932292375 28.382955023044097 23.970584045076684 32-33 21.642266157583183 27.154730273608628 29.640674974890125 21.562328593918068 34-35 24.79361766353201 25.194227794809965 28.744533795413684 21.267620746244337 36-37 23.05299313729266 24.238845456959872 32.50167688529659 20.206484520450875 38-39 21.72594577975416 27.0167510553314 31.43354757211997 19.82375559279447 40-41 21.0256535831315 28.389527532412025 29.6404847556632 20.944334128793276 42-43 19.698542442402356 29.12546321535287 26.70500091157175 24.470993430673023 44-45 22.183817749841225 25.359892597083462 27.468905845031088 24.98738380804422 46-47 24.617562389865537 28.114929847853055 27.410247369016233 19.85726039326518 48-49 23.36720510676643 26.802754797388207 28.447681733550954 21.38235836229441 50-51 18.77810970967584 28.218653583306246 32.2876217104238 20.715614996594113 52-53 21.732599388814887 30.43279367872178 26.390593056139277 21.444013876324057 54-55 19.267312459485836 26.426410249104237 28.96488980147169 25.34138748993824 56-57 23.28967894973038 25.943334484944756 27.51688683814992 23.25009972717494 58-59 26.61005513269177 25.556062848641876 26.41725655255837 21.416625466107988 60-61 22.059472696880512 26.95128577912857 31.387039563650514 19.602201960340405 62-63 21.06326976913159 27.053645206328405 30.142093415869443 21.740991608670566 64-65 20.03847075092092 31.142676132462977 27.875756526137046 20.94309659047906 66-67 21.75557641651253 26.702109021973087 26.321825698319547 25.22048886319484 68-69 19.801686177268774 27.859724013300468 28.080174116179407 24.25841569325135 70-71 23.891425599965807 27.515751551113738 26.219008889965313 22.373813958955143 72-73 22.306765242230185 24.45003134282641 30.91101664731485 22.33218676762856 74-75 19.548752341807766 31.51305268444185 27.201944857995624 21.736250115754757 76-77 21.517347040891995 26.210312184551892 27.039144595561122 25.233196178994994 78-79 21.5578511624014 26.809738189220017 28.808691052950724 22.82371959542786 80-81 23.607336714559455 26.67054148294309 27.524922889523662 22.197198912973793 82-83 21.675300784282996 25.694016547587673 30.373870055491047 22.25681261263828 84-85 20.484852154463148 27.64049956191277 29.098408461138458 22.77623982248563 86-87 20.854067362167843 28.524814625409796 27.6078284678123 23.013289544610057 88-89 20.419375095428872 27.934933773761088 25.96930582776148 25.67638530304856 90-91 21.57011500619072 24.833574232324295 27.559410336442685 26.036900425042298 92-93 23.123793479267434 27.991792987754927 27.716074061674135 21.168339471303504 94-95 21.786558913828596 26.895127971335558 27.979238048766586 23.339075066069253 96-97 17.527592600301364 27.2871845363457 30.91533238339829 24.269890479954643 98-99 20.796254710328174 28.77505200059836 25.872011746436534 24.55668154263693 100-101 20.025296939635723 25.641845287532313 27.93786979021393 26.39498798261804 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3671.0 1 2383.0 2 1213.0 3 1262.5 4 1042.0 5 1050.0 6 1787.5 7 3396.5 8 4955.0 9 5502.5 10 4602.5 11 3500.5 12 3356.0 13 3458.5 14 3633.5 15 3715.0 16 3791.0 17 3970.0 18 4190.5 19 4430.0 20 4789.0 21 5392.5 22 6180.5 23 7076.0 24 8031.0 25 9640.5 26 12270.5 27 14863.5 28 16278.0 29 17858.5 30 20579.0 31 23275.5 32 26569.5 33 30681.5 34 35752.5 35 39272.0 36 42429.5 37 46614.5 38 47381.5 39 46174.5 40 45592.0 41 46324.5 42 47378.5 43 49031.5 44 51686.5 45 55071.0 46 66997.5 47 145675.5 48 310943.0 49 296300.5 50 137983.0 51 75447.5 52 54174.0 53 45401.0 54 41498.0 55 38594.5 56 34774.0 57 30646.0 58 27534.5 59 24718.5 60 21683.5 61 18395.0 62 15197.5 63 11867.5 64 8675.5 65 6289.5 66 4231.0 67 3247.5 68 2242.0 69 1185.0 70 825.5 71 633.0 72 489.0 73 419.5 74 319.5 75 225.5 76 138.0 77 58.0 78 27.0 79 10.5 80 4.0 81 2.5 82 1.0 83 0.5 84 0.0 85 1.0 86 1.0 87 0.0 88 0.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39837444704843183 2 0.0 3 0.0 4 0.0 5 3.116474216963593E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001291110747027774 16-17 0.0023596161928438626 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0024041372530861998 36-37 0.006611377445987049 38-39 0.01199842573530983 40-41 0.0021370108916321778 42-43 3.339079518175278E-4 44-45 2.893868915751907E-4 46-47 4.4521060242337033E-4 48-49 4.4521060242337033E-4 50-51 8.013790843620667E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 6.678159036350556E-5 60-61 0.0017140608193299757 62-63 0.0 64-65 2.671263614540222E-4 66-67 0.001758581879572313 68-69 0.0018476240000569872 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.12428053966648384 78-79 8.904212048467407E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14411467200444497 88-89 0.4235956276757157 90-91 0.32464757128712163 92-93 0.0 94-95 0.0 96-97 4.229500723022019E-4 98-99 0.0 100-101 2.893868915751907E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2246128.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.806883451841227 #Duplication Level Percentage of deduplicated Percentage of total 1 71.11376068491614 22.619070979272564 2 14.41943010500214 9.172742655835474 3 5.627706772680284 5.369994402593379 4 2.8055621027833553 3.5694474728053107 5 1.5463814962912263 2.459278801230944 6 0.9695421515327894 1.8502868529270495 7 0.6241671666993686 1.3896968627970938 8 0.45266040000407715 1.1518173268954808 9 0.33671726531623564 0.9638934132722586 >10 1.829297356993427 10.767101689915577 >50 0.16618311473188832 3.681365363572924 >100 0.09149724807631121 5.336402070299644 >500 0.007005793021635094 1.464276543704088 >1k 0.005744750277740776 4.439045079609377 >5k 0.0014011586043270188 3.2849958618343176 >10k+ 0.0029424330690867393 22.480584623434556 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 99252 4.418804271172435 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 48854 2.1750318770791335 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 36200 1.6116623807726007 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 31886 1.4195985268871587 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 26245 1.1684552260601355 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 25423 1.1318589145409346 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 24616 1.0959304189253685 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 22932 1.0209569534772729 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 22105 0.9841380366568602 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 21360 0.9509698467763191 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 18503 0.8237731776639622 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 16409 0.7305460775165084 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 16341 0.7275186454200295 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 14175 0.6310860289351274 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 12960 0.576992940740688 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 11935 0.5313588539922925 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 11697 0.5207628416546163 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11297 0.5029544175576814 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 11192 0.49827970623223605 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 10736 0.47797810276173036 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10310 0.45901213109849487 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 8771 0.39049421938553813 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 8639 0.38461743943354965 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 8550 0.38065506507198166 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 8377 0.3729529216500574 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 8357 0.3720625004452106 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 7048 0.3137844325879914 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6701 0.2983356246839005 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 6088 0.27104421475534785 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 5737 0.2554173226102876 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 5442 0.24228360983879815 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 4773 0.2124990205366747 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 4667 0.20777978815098697 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 4250 0.1892145060299324 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 4105 0.18275895229479353 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 3967 0.17661504598135103 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 3811 0.16966976058354644 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 3773 0.16797796029433765 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3773 0.16797796029433765 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 3619 0.16112171701701772 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 3409 0.15177229436612696 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 3384 0.15065926786006853 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3213 0.1430461665586289 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 3146 0.14006325552239232 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3014 0.13418647557040383 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 2726 0.12136441022061076 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 2716 0.12091919961818738 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 2607 0.11606640405177265 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 2599 0.11571023556983397 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2351 0.10466901262973438 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2288 0.10186418583446714 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 4.4521060242337034E-5 0.0 9 0.0 0.0 0.0 8.904212048467407E-5 0.0 10-11 0.0 0.0 0.0 1.7808424096934814E-4 0.0 12-13 0.0 0.0 0.0 2.2260530121168517E-4 0.0 14-15 0.0 0.0 0.0 3.116474216963592E-4 0.0 16-17 0.0 0.0 0.0 5.787737831503814E-4 0.0 18-19 0.0 0.0 0.0 6.90076433756224E-4 0.0 20-21 0.0 0.0 0.0 9.349422650890778E-4 0.0 22-23 0.0 0.0 0.0 0.001513716048239459 0.0 24-25 0.0 0.0 0.0 0.0025154399036920426 0.0 26-27 0.0 0.0 0.0 0.0038955927712044907 0.0 28-29 0.0 0.0 0.0 0.009416204241254283 0.0 30-31 0.0 0.0 0.0 0.021704016868139303 0.0 32-33 0.0 0.0 0.0 0.050887571856991234 0.0 34-35 0.0 0.0 0.0 0.09485211884629906 0.0 36-37 0.0 0.0 0.0 0.1556901476674526 0.0 38-39 0.0 0.0 0.0 0.24693606063412238 0.0 40-41 0.0 0.0 0.0 0.3441700562033865 0.0 42-43 0.0 0.0 0.0 0.4509315586645106 0.0 44-45 0.0 0.0 0.0 0.583470755005948 0.0 46-47 0.0 0.0 0.0 0.7527398260473135 0.0 48-49 0.0 0.0 0.0 0.9062484417628915 0.0 50-51 0.0 0.0 0.0 1.0841100774310282 0.0 52-53 0.0 0.0 0.0 1.2506633637976108 0.0 54-55 0.0 0.0 0.0 1.448025223851891 0.0 56-57 0.0 0.0 0.0 1.7341843385595122 0.0 58-59 0.0 0.0 0.0 2.0029579792425007 0.0 60-61 0.0 0.0 0.0 2.3261363555416255 0.0 62-63 0.0 0.0 0.0 2.637138221864471 0.0 64-65 0.0 0.0 0.0 2.926013121246875 0.0 66-67 0.0 0.0 0.0 3.2805120634264835 0.0 68-69 0.0 0.0 0.0 3.700145316740631 0.0 70-71 0.0 0.0 0.0 4.136718833476988 0.0 72-73 0.0 0.0 0.0 4.557042163224892 0.0 74-75 0.0 0.0 0.0 5.0526506058425875 0.0 76-77 0.0 0.0 0.0 5.491183049229607 0.0 78-79 0.0 0.0 0.0 6.133644209056652 0.0 80-81 0.0 0.0 0.0 6.818289073463311 0.0 82-83 0.0 0.0 0.0 7.394124466637698 0.0 84-85 0.0 0.0 0.0 8.05481700063398 0.0 86-87 0.0 0.0 0.0 8.728554205281267 0.0 88-89 0.0 0.0 0.0 9.438642855616422 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGCGCG 35 2.8021788E-4 54.232025 4 GTAAGCG 710 0.0 39.432793 94-95 TTTGCGT 1590 0.0 29.546219 3 CTTTGCG 1610 0.0 29.179186 2 CGCTTAC 115 3.7773625E-6 29.0 1 TTGCGTT 1690 0.0 28.078709 4 GTAGCGC 95 0.0012340173 24.975273 3 GCTTTGC 1925 0.0 24.50204 1 GTACATG 10940 0.0 21.861715 1 GAGTACT 8935 0.0 21.724056 12-13 ACATGCG 110 0.0028947855 21.569553 3 TACATGG 11240 0.0 20.813467 2 ACATGGG 11125 0.0 20.34615 3 GGACCGT 140 4.8862165E-4 20.33701 6 AGTACTT 9210 0.0 20.250937 12-13 GTACTTT 9835 0.0 19.8326 14-15 TACATCG 145 6.189651E-4 19.635733 5 GCTTATG 610 0.0 18.744732 1 GATATAG 665 0.0 18.627285 1 CATGGGA 7360 0.0 18.504099 4 >>END_MODULE