FastQCFastQC Report
Fri 27 May 2016
SRR938969_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938969_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2943800
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT128140.43528772335077115No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115680.3929614783613017No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92700.31489910999388543No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT74340.25253074257762076No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59760.20300292139411644No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA58670.1993002242000136No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT57160.19417079964671513No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA48350.16424349480263606No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC45200.15354303960866908No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT37600.12772606834703445No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT37070.12592567429852572No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC31960.10856715809497927No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT31660.10754806712412529No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC29820.10129764250288742No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG124550.022.5585021
TACATGG120400.022.3817422
GAGTACT98550.021.99899712-13
TACCGGC1303.013226E-421.8467667
ACATGGG122600.020.8598633
GTACTTT107650.020.0306114-15
AGTACTT105750.019.87451712-13
TTTGCGT7500.019.5753063
GTATAGG6550.019.563871
CATGGGG56350.017.901684
AGTACAT92150.017.8851262
GTCTAGG8250.017.8336411
CTTTGCG8700.016.8752632
GTGTAGC16650.016.8178351
ACTTTTT130650.016.63259516-17
TACTTTT126500.016.39092414-15
CATGGGA66350.016.1315334
CCTATAC6200.016.0411593
GTAGCCC14200.015.3418463
GTATAGC13650.015.2986221
Produced by FastQC (version 0.11.5)