FastQCFastQC Report
Fri 27 May 2016
SRR938969_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938969_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2943800
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT138720.4712276649228888No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109910.3733609620218765No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93790.3186018071879883No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT69770.23700659012161154No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61990.21057816427746448No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA61920.21034037638426525No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA52770.17925810177321827No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT52140.1771180107344249No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC43640.14824376656022828No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT39590.1344860384536993No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC34490.11716149194918132No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT32310.10975609756097562No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT32300.10972212786194714No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC32290.10968815816291867No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT101850.021.03769312-13
TACATGG126900.020.1183572
GTACATG130700.020.0838871
GTAAGCG3000.019.77279394-95
AGTACTT110350.019.30969212-13
TTTGCGT6700.019.123213
GTACTTT111850.019.07227314-15
ACATGGG128350.018.9297963
CATGGGG56150.016.7335194
TACTTTT131000.016.1030914-15
ACTTTTT130800.016.09088116-17
AGTACAT98600.016.0265182
CTTTGCG8850.015.5498642
TATAGGG9500.014.9854412
TACACTG23500.014.7409975
AGAGTAC243950.014.69696410-11
TCTATAC9400.014.6400313
CATGGGA68650.014.4469994
GTATAGA8950.013.8395551
CATGGGT20950.013.8171254
Produced by FastQC (version 0.11.5)