##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938969_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2943800 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19839832869081 33.0 31.0 34.0 30.0 34.0 2 32.44126842856172 34.0 31.0 34.0 31.0 34.0 3 32.534353896324475 34.0 31.0 34.0 31.0 34.0 4 36.06996195393709 37.0 35.0 37.0 35.0 37.0 5 35.99963448603845 37.0 35.0 37.0 35.0 37.0 6 36.03960697058224 37.0 35.0 37.0 35.0 37.0 7 35.9801538827366 37.0 35.0 37.0 35.0 37.0 8 35.953072559277125 37.0 35.0 37.0 35.0 37.0 9 37.63289625653917 39.0 37.0 39.0 35.0 39.0 10-11 37.545870643386095 39.0 37.0 39.0 35.0 39.0 12-13 37.46247859908961 39.0 37.0 39.0 34.0 39.0 14-15 38.76717507982879 40.0 38.0 41.0 34.5 41.0 16-17 38.86241133908553 40.0 38.0 41.0 35.0 41.0 18-19 38.91121017052789 40.0 38.0 41.0 35.0 41.0 20-21 38.88133704735376 40.0 38.0 41.0 35.0 41.0 22-23 38.81121611522522 40.0 38.0 41.0 34.0 41.0 24-25 38.7380102248794 40.0 38.0 41.0 34.0 41.0 26-27 38.5716361505537 40.0 38.0 41.0 34.0 41.0 28-29 38.42287842244718 40.0 38.0 41.0 34.0 41.0 30-31 38.2361257558258 40.0 38.0 41.0 34.0 41.0 32-33 38.134124261159045 40.0 38.0 41.0 33.0 41.0 34-35 38.03356766764047 40.0 37.5 41.0 33.0 41.0 36-37 37.90352996127454 40.0 37.0 41.0 33.0 41.0 38-39 37.75716217134317 40.0 37.0 41.0 33.0 41.0 40-41 37.5932412188328 40.0 37.0 41.0 33.0 41.0 42-43 37.41611726340105 40.0 37.0 41.0 32.0 41.0 44-45 37.16816699504042 40.0 36.5 41.0 31.0 41.0 46-47 36.86749575378762 40.0 36.0 41.0 31.0 41.0 48-49 36.53384197296012 39.5 36.0 41.0 30.0 41.0 50-51 36.27563370473538 39.0 35.5 41.0 30.0 41.0 52-53 36.018439262178134 39.0 35.0 40.0 29.0 41.0 54-55 35.73010275833956 39.0 35.0 40.0 28.0 41.0 56-57 35.5999859025749 38.5 35.0 40.0 28.0 41.0 58-59 35.32972620422583 38.0 34.5 40.0 28.0 41.0 60-61 34.95701100618248 38.0 34.0 40.0 27.0 41.0 62-63 34.54303926897208 37.5 34.0 40.0 26.0 41.0 64-65 34.13688599769006 37.0 33.5 39.5 26.0 41.0 66-67 33.67856495006454 36.0 33.0 39.0 25.5 40.5 68-69 33.19234153135403 36.0 32.5 39.0 24.0 40.0 70-71 32.65714552619064 35.0 32.0 38.0 22.5 40.0 72-73 32.13512789591684 35.0 31.0 37.5 21.5 39.5 74-75 31.612534479244513 35.0 31.0 37.0 20.0 39.0 76-77 29.704270161016375 32.5 28.5 35.0 18.0 37.5 78-79 30.65700302330321 34.0 30.0 36.0 18.0 38.0 80-81 30.63489554317549 34.0 30.5 35.5 18.0 37.0 82-83 30.218008866091445 34.0 30.0 35.0 14.5 37.0 84-85 29.753692166587406 34.0 30.0 35.0 7.5 36.0 86-87 29.06304300563897 34.0 29.0 35.0 3.5 36.0 88-89 28.35100278551532 33.0 28.0 35.0 2.0 35.5 90-91 27.761793430260205 33.0 26.5 35.0 2.0 35.0 92-93 27.348704395679057 33.0 26.0 35.0 2.0 35.0 94-95 26.787922922752905 32.5 25.5 34.0 2.0 35.0 96-97 25.921339085535703 32.0 24.5 34.0 2.0 35.0 98-99 24.707179835586658 32.0 11.0 34.0 2.0 35.0 100-101 19.146152591888036 24.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10720662147634386 1101 2 0.11292125147575405 1101 3 0.11966747124051835 1101 4 0.07784451127955094 1101 5 0.10305651124332371 1101 6 0.11238791171835061 1101 7 0.2192075119075696 1101 8 0.07205255443260228 1101 9 0.1835913996532028 1101 10-11 0.2020373586977371 1101 12-13 0.24535892042637641 1101 14-15 0.20948734940227354 1101 16-17 0.16796160650982728 1101 18-19 0.27732851616003273 1101 20-21 0.2687329098698612 1101 22-23 0.2932538513759013 1101 24-25 0.18198309950140157 1101 26-27 0.24019981260476442 1101 28-29 0.2207472716600165 1101 30-31 0.14429660213342288 1101 32-33 0.2786758930648503 1101 34-35 0.32723152849356296 1101 36-37 0.3575033387402229 1101 38-39 0.28408579106510956 1101 40-41 0.2427287241306928 1101 42-43 0.3125640707222388 1101 44-45 0.3846000691539331 1101 46-47 0.24583398618264596 1101 48-49 0.23054737923506963 1101 50-51 0.20314958487837487 1101 52-53 0.12078988802810642 1101 54-55 0.1700794284154341 1101 56-57 0.24458445258826345 1101 58-59 0.273093547310701 1101 60-61 0.27580039019284897 1101 62-63 0.20725226982301592 1101 64-65 0.22270326703831245 1101 66-67 0.2158406310626404 1101 68-69 0.2704914525120472 1101 70-71 0.28944124386369197 1101 72-73 0.2512285805628238 1101 74-75 0.23705953961242798 1101 76-77 -0.041408167074692415 1101 78-79 0.29059504473713815 1101 80-81 0.3369840813853777 1101 82-83 0.37870217741805945 1101 84-85 0.3943709924490406 1101 86-87 0.4700627997900533 1101 88-89 0.42497090576270224 1101 90-91 0.5227584159303511 1101 92-93 0.46134922647102883 1101 94-95 0.41492469105206453 1101 96-97 0.6143310879678872 1101 98-99 0.7766478280615097 1101 100-101 1.0901694563387174 1102 1 0.05718182735652988 1102 2 0.09749500767860297 1102 3 0.12507965926845088 1102 4 0.10345945736284534 1102 5 0.09678555180729376 1102 6 0.0675708715578125 1102 7 0.12343744313169935 1102 8 0.05035325000283564 1102 9 0.065241130675318 1102 10-11 0.20204162289083172 1102 12-13 0.26404072114691246 1102 14-15 0.30859770276421017 1102 16-17 0.23910225220506476 1102 18-19 0.16289580463257636 1102 20-21 0.24075096582299693 1102 22-23 0.2134593657068038 1102 24-25 0.20123889769160996 1102 26-27 0.19673588125110797 1102 28-29 0.18341222177266303 1102 30-31 0.24323898336760408 1102 32-33 0.0930631693059425 1102 34-35 0.21105439119346414 1102 36-37 0.12961491030178252 1102 38-39 0.17501471524750656 1102 40-41 0.2227361475346612 1102 42-43 0.07027855221861046 1102 44-45 0.11257662424953452 1102 46-47 0.24450535013609453 1102 48-49 0.2670953455981291 1102 50-51 0.22199243613788155 1102 52-53 0.19632605159908678 1102 54-55 0.22423461884070406 1102 56-57 0.14645412203790187 1102 58-59 0.1309446313056597 1102 60-61 0.09642698432126195 1102 62-63 0.09747389415181118 1102 64-65 0.13562288217820395 1102 66-67 0.002406766596244836 1102 68-69 0.10357017025805959 1102 70-71 0.15402561234535028 1102 72-73 0.2840334505926947 1102 74-75 0.3974063013223912 1102 76-77 0.10967658184714679 1102 78-79 0.2980393370714296 1102 80-81 0.23197820297890814 1102 82-83 0.2908154892648014 1102 84-85 0.5299485397049502 1102 86-87 0.6325701477981411 1102 88-89 0.2898642119436339 1102 90-91 0.49943612253112235 1102 92-93 0.6332662272279563 1102 94-95 0.7712002484006426 1102 96-97 0.6632727152432984 1102 98-99 0.9844887397929618 1102 100-101 0.9894361758072172 1103 1 0.09151941689852094 1103 2 0.02776890317745284 1103 3 0.12864323039561754 1103 4 0.058790848020095154 1103 5 0.1184972765032839 1103 6 0.07621257185169128 1103 7 0.07228380659238809 1103 8 0.1726054081023065 1103 9 0.04584694892984231 1103 10-11 0.06913397657430664 1103 12-13 0.0806180184291918 1103 14-15 0.05462829805212266 1103 16-17 0.10636800300091664 1103 18-19 0.10096543833304139 1103 20-21 0.10519516854233046 1103 22-23 0.23154947217361865 1103 24-25 0.14207054397936503 1103 26-27 0.09680153153120585 1103 28-29 0.048083589593083786 1103 30-31 0.09389805363660031 1103 32-33 0.16381626099630608 1103 34-35 0.17827408880775408 1103 36-37 0.28531528455675925 1103 38-39 0.13943847744886284 1103 40-41 0.2901135149695975 1103 42-43 0.2661480792017059 1103 44-45 0.23297803484371826 1103 46-47 0.11718814827432311 1103 48-49 -0.031386139732944685 1103 50-51 0.07282527561606145 1103 52-53 0.17418889621965405 1103 54-55 0.3356521241102186 1103 56-57 0.43130737573920896 1103 58-59 0.2287535020439435 1103 60-61 0.25010079532932394 1103 62-63 0.2848333190786718 1103 64-65 0.1841438097708945 1103 66-67 0.11249154513290449 1103 68-69 0.21461731517165816 1103 70-71 0.21109025473923282 1103 72-73 0.044283725386186745 1103 74-75 0.13373168162393867 1103 76-77 0.03604222054264028 1103 78-79 0.16158430583886485 1103 80-81 0.1764402117708137 1103 82-83 0.24549428413750718 1103 84-85 0.2730627022303871 1103 86-87 0.2687788509440203 1103 88-89 0.2603277254950278 1103 90-91 0.319726821921801 1103 92-93 0.31771939003841254 1103 94-95 0.6166245367468903 1103 96-97 0.49230812372745447 1103 98-99 0.5493142673208062 1103 100-101 0.9821696212822566 1104 1 0.08753920830932316 1104 2 0.10249798480187877 1104 3 0.0929160908961606 1104 4 0.06748475066576987 1104 5 0.05568245033006036 1104 6 0.10203277415108403 1104 7 0.14154471010779446 1104 8 0.13418654297328914 1104 9 0.14648926146985275 1104 10-11 0.17902006169084927 1104 12-13 0.08745037207271622 1104 14-15 0.09468712827465708 1104 16-17 0.0725649199202607 1104 18-19 0.1720915533945444 1104 20-21 0.1612038893036285 1104 22-23 0.11433890959222026 1104 24-25 0.14897053260105508 1104 26-27 0.05636643596078983 1104 28-29 0.10030255405029465 1104 30-31 0.03466373042344628 1104 32-33 0.07572621906262356 1104 34-35 0.1671673022114888 1104 36-37 0.20957497454926965 1104 38-39 0.19672347269000312 1104 40-41 0.18016248074043517 1104 42-43 0.059238397047032265 1104 44-45 0.112490105856061 1104 46-47 0.1190949907365848 1104 48-49 0.18591332499846658 1104 50-51 0.04055594385452821 1104 52-53 0.0038404369053850473 1104 54-55 0.11140579019461683 1104 56-57 0.03636073412108942 1104 58-59 0.20915608399092633 1104 60-61 0.26865109206864446 1104 62-63 0.2529763897652799 1104 64-65 0.18929761952468738 1104 66-67 0.19711266961203222 1104 68-69 0.2762354661534445 1104 70-71 0.29098156996852964 1104 72-73 0.17631941126101935 1104 74-75 0.38630510708409105 1104 76-77 0.3062438362616895 1104 78-79 0.417063105325326 1104 80-81 0.39962648338409323 1104 82-83 0.4497972116821316 1104 84-85 0.5283068547348115 1104 86-87 0.5678109138303888 1104 88-89 0.7058815680652124 1104 90-91 0.6608069647317159 1104 92-93 0.6943224244804895 1104 94-95 0.8062337042408849 1104 96-97 0.5999038980307532 1104 98-99 0.9041970732396756 1104 100-101 1.1270038228012886 1105 1 -0.005572995936589109 1105 2 0.011289252662244564 1105 3 0.054600003690829624 1105 4 0.05323832154899577 1105 5 0.04870884016438026 1105 6 0.006514769535947096 1105 7 -0.0039716058091130435 1105 8 0.03609196540709547 1105 9 0.006374639293490247 1105 10-11 0.066532899365086 1105 12-13 0.046202371753054194 1105 14-15 0.05750211211785228 1105 16-17 0.03518308717369223 1105 18-19 0.07729493212293193 1105 20-21 0.04698093547073512 1105 22-23 0.006756702097348466 1105 24-25 -0.024641797464759918 1105 26-27 -0.006530556776858987 1105 28-29 0.14062474340236264 1105 30-31 0.16929924036988098 1105 32-33 0.15514974029812123 1105 34-35 0.1525738575001796 1105 36-37 0.2826120055609067 1105 38-39 0.3040426238123217 1105 40-41 0.3655610920120935 1105 42-43 0.39045621961574994 1105 44-45 0.24626200125570818 1105 46-47 0.251192101825815 1105 48-49 0.306348190956264 1105 50-51 0.42001440449430305 1105 52-53 0.4184535513834291 1105 54-55 0.3893143826242067 1105 56-57 0.3675847406551611 1105 58-59 0.3276529903433314 1105 60-61 0.45121494983553134 1105 62-63 0.5388828710842333 1105 64-65 0.2961318050560138 1105 66-67 0.41701756973861137 1105 68-69 0.4415012268216145 1105 70-71 0.5743148081639333 1105 72-73 0.6395242227666174 1105 74-75 0.4799541270920713 1105 76-77 0.16539784125928492 1105 78-79 0.3364040991646675 1105 80-81 0.3826237895536444 1105 82-83 0.339668042916486 1105 84-85 0.36169001655824573 1105 86-87 0.48955116730637016 1105 88-89 0.5764812604842895 1105 90-91 0.5061068656455596 1105 92-93 0.686421100588646 1105 94-95 0.7948756626361195 1105 96-97 0.8435385759959999 1105 98-99 1.1821173200655188 1105 100-101 1.2216552398093796 1106 1 0.01845779108246859 1106 2 -0.03692145707677241 1106 3 -0.03380155310398436 1106 4 0.00138352351884663 1106 5 0.05235059638825845 1106 6 0.02074924164104175 1106 7 0.08663608660801003 1106 8 0.08502200129061777 1106 9 0.03689452005022531 1106 10-11 0.11081541671974549 1106 12-13 0.01821634036949149 1106 14-15 -0.06917175830455591 1106 16-17 -0.06743358556800416 1106 18-19 -0.07359477485514532 1106 20-21 -1.1766969651461068E-4 1106 22-23 -0.020231904121665423 1106 24-25 0.0549339925055321 1106 26-27 0.008101824303459182 1106 28-29 0.13148980276164224 1106 30-31 -0.031476650065869194 1106 32-33 0.08135415392196421 1106 34-35 -0.013783868557005974 1106 36-37 -0.060312595673082114 1106 38-39 0.05836260349535394 1106 40-41 -0.031079530127477994 1106 42-43 0.058240762720636496 1106 44-45 0.09306628787516757 1106 46-47 -0.051058273112296604 1106 48-49 0.07970337809182837 1106 50-51 0.04160932394587036 1106 52-53 -0.0303832251305991 1106 54-55 0.07064829477663181 1106 56-57 0.07708611902351237 1106 58-59 -0.02284173440393289 1106 60-61 0.0034678317459437835 1106 62-63 0.1388306094570666 1106 64-65 0.13122742331152182 1106 66-67 0.06352940346408076 1106 68-69 0.06249814592757019 1106 70-71 0.11388503382175941 1106 72-73 0.2998827641145212 1106 74-75 0.3628585815970524 1106 76-77 0.0652655780649738 1106 78-79 0.35343110520309295 1106 80-81 0.3590809293058079 1106 82-83 0.4659053503489474 1106 84-85 0.49823517785684146 1106 86-87 0.7168922188494911 1106 88-89 0.7601663045405367 1106 90-91 0.9330230844970124 1106 92-93 0.865118435805357 1106 94-95 0.8446134100130678 1106 96-97 0.7754083972033996 1106 98-99 0.7704748007892341 1106 100-101 0.6248596401404392 1107 1 0.005016182883949227 1107 2 0.05199874954312378 1107 3 -0.004515553791918592 1107 4 -0.013716939717987486 1107 5 -0.025470507121724495 1107 6 -0.03111456379185995 1107 7 -0.024153748444788903 1107 8 -0.022429182636471978 1107 9 -0.008868729704410327 1107 10-11 0.02747090904207994 1107 12-13 -0.0014511805608492523 1107 14-15 0.010561343975346915 1107 16-17 0.006093908698943551 1107 18-19 -0.042216116838552864 1107 20-21 -0.01933651997276087 1107 22-23 0.03599139965624687 1107 24-25 -0.054380192764448054 1107 26-27 -0.026013458601447326 1107 28-29 -0.016010269837856583 1107 30-31 -0.07983340825726515 1107 32-33 0.0374998948307379 1107 34-35 -0.028243200171935712 1107 36-37 -0.03949030893598149 1107 38-39 -0.06806909611606216 1107 40-41 -0.15513702383562844 1107 42-43 -0.05397416278650269 1107 44-45 -0.07377382698359014 1107 46-47 -0.10632656092468551 1107 48-49 -0.15591072858557453 1107 50-51 -0.29306103559574836 1107 52-53 -0.3154183812183575 1107 54-55 -0.35949115062802406 1107 56-57 -0.16884862751054186 1107 58-59 -0.12388235413866511 1107 60-61 -0.03420974343328709 1107 62-63 0.024206248491694282 1107 64-65 -0.03136975732908809 1107 66-67 -0.049036602780518024 1107 68-69 -0.06627698434369478 1107 70-71 0.007331480443895089 1107 72-73 0.07417099694566787 1107 74-75 0.07766422725761046 1107 76-77 0.095726242965263 1107 78-79 0.23019360975719394 1107 80-81 0.07152110414158486 1107 82-83 0.05421878717888262 1107 84-85 0.2431947772511016 1107 86-87 0.18514524133126642 1107 88-89 0.5035122861903965 1107 90-91 0.46364520372886986 1107 92-93 0.5098095732641355 1107 94-95 0.5160820752039861 1107 96-97 0.5435669767107214 1107 98-99 0.32027377250273403 1107 100-101 0.3839656702254395 1108 1 0.0856451508095546 1108 2 0.08917870718660481 1108 3 0.06077848343672798 1108 4 0.03614742828006001 1108 5 0.006043246754764198 1108 6 0.09456245695707821 1108 7 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-0.7561029535331585 2316 56-57 -0.8587734125243074 2316 58-59 -1.0310096106889048 2316 60-61 -0.8630351597174908 2316 62-63 -0.7682389883957583 2316 64-65 -0.818610988789537 2316 66-67 -0.8395501340300129 2316 68-69 -0.8054717622717718 2316 70-71 -0.7385305881745623 2316 72-73 -0.6027413337627365 2316 74-75 -0.8278207901345311 2316 76-77 -0.8572349004877182 2316 78-79 -0.9132845876597528 2316 80-81 -0.880173220866066 2316 82-83 -0.930541446809876 2316 84-85 -1.2898863297266914 2316 86-87 -1.29985962001469 2316 88-89 -1.3844426975447313 2316 90-91 -1.3954548735695518 2316 92-93 -1.4259994734691972 2316 94-95 -1.3228554942438109 2316 96-97 -1.229511684496746 2316 98-99 -1.4325217027215125 2316 100-101 -0.9387157797640917 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 6.0 4 29.0 5 52.0 6 136.0 7 346.0 8 811.0 9 1553.0 10 2750.0 11 4036.0 12 6001.0 13 8260.0 14 10208.0 15 13539.0 16 17748.0 17 24750.0 18 24660.0 19 17956.0 20 17073.0 21 18291.0 22 19839.0 23 22013.0 24 25428.0 25 30161.0 26 35847.0 27 43461.0 28 52539.0 29 64120.0 30 76670.0 31 93630.0 32 116166.0 33 147319.0 34 198542.0 35 283851.0 36 442109.0 37 657578.0 38 441476.0 39 24843.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.46831235830816 19.938444306729345 15.252905972382477 22.34033736258002 2 15.192370405598208 21.52924791086351 39.902574903186355 23.375806780351926 3 19.632379917113933 25.162578979550243 30.55336639717372 24.6516747061621 4 12.846830627080644 17.417079964671515 37.98226781710714 31.753821591140703 5 13.204871254840683 38.20276513350092 34.581187580678034 14.011176030980366 6 30.609756097560975 37.186867314355595 17.541069366125416 14.662307221958013 7 27.279196956314966 33.57860588355187 21.26961750118894 17.872579658944222 8 25.240029893335148 35.309599836945445 20.054215639649435 19.39615463006998 9 25.66736870711325 17.66767443440451 21.167130919220057 35.49782593926218 10-11 24.65892723690468 26.743715605679736 27.54774441198451 21.049612745431077 12-13 26.09316189958557 24.304351518445547 27.81714111012976 21.78534547183912 14-15 22.66605498073648 25.674690317012853 27.06474149786847 24.594513204382192 16-17 21.39785364474955 28.82736197846647 27.691695701497537 22.083088675286444 18-19 21.304328108835154 28.126380553785822 29.48396651053582 21.085324826843205 20-21 22.54083157823222 26.72888783205381 29.21200489163666 21.518275698077318 22-23 21.9036959032543 26.636116584007063 29.287281744683742 22.172905768054896 24-25 22.219236659061973 26.740321000504114 29.382283962611538 21.658158377822375 26-27 22.017701952867373 26.59571414425473 29.833010026667917 21.55357387620998 28-29 21.53048780487805 27.491388681296286 29.56544262517834 21.412680888647326 30-31 21.86554793124533 26.973316801413137 29.296436578571917 21.864698688769618 32-33 21.67621781371017 27.0470140634554 29.33456756573137 21.942200557103064 34-35 22.358094387781968 26.825041881083205 29.206156850474596 21.61070688066023 36-37 22.311162062236217 26.541222927802433 29.63213767132934 21.515477338632007 38-39 22.053417821975927 26.968481867918324 29.50057618246503 21.477524127640724 40-41 22.06497163289582 27.117816946612162 29.339862894169922 21.4773485263221 42-43 21.629865189681738 27.30517457126847 28.81705376768911 22.247906471360686 44-45 22.021984532322104 27.11976537421722 28.73055011606788 22.127699977392794 46-47 22.408482341900886 27.358219285693053 28.283752510605048 21.949545861801013 48-49 22.19654979834984 27.283545401180547 28.51814307921427 22.00176172125534 50-51 21.97358871477976 27.567944542041957 28.699466111507334 21.75900063167095 52-53 22.18540661729737 27.823102792309264 28.036840138596368 21.954650451796997 54-55 21.857514097425096 27.455941300360077 28.281116244310073 22.40542835790475 56-57 22.254891636660098 27.671614919491816 27.87273252258985 22.200760921258237 58-59 22.479478737512636 27.329779847379694 27.99824103464394 22.19250038046373 60-61 22.048174660532805 27.36775237854212 28.672152267459982 21.911920693465092 62-63 22.04305038454222 27.357834877843302 28.537788406663587 22.06132633095089 64-65 21.87506581687317 27.872554075142997 28.238614784966508 22.013765323017324 66-67 21.91494468138014 27.47248846230084 28.019585425853244 22.592981430465777 68-69 21.760230564780443 27.570499040672235 28.24275884184952 22.426511552697804 70-71 22.287649783094867 27.332254568374843 27.834174112120785 22.545921536409505 72-73 21.97994089272369 27.077128201644136 28.232590529247908 22.710340376384266 74-75 21.64111012976425 28.050428018207757 27.700896800054352 22.607565051973637 76-77 22.00376427690675 27.302142978940143 27.80330420617398 22.890788537979127 78-79 21.9166150448528 27.415862671424197 28.104863140054583 22.56265914366842 80-81 22.0651044417865 27.539844003977183 27.759645464202137 22.63540609003418 82-83 21.893827705686526 27.40741558529791 28.065051973639516 22.633704735376046 84-85 21.775506862567923 27.507578650151032 27.758852855292957 22.958061631988087 86-87 21.694007074980924 27.51134961857393 27.869365822211524 22.925277484233618 88-89 21.881964850408835 27.33171078904377 27.60447019709375 23.181854163453654 90-91 21.727249769331596 27.048728454820964 27.911413784583424 23.312607991264013 92-93 21.81452544330457 27.497927848359264 27.718136422311296 22.969410286024868 94-95 21.745622406917388 27.390714524353893 27.637235184344426 23.22642788438429 96-97 21.248080712004892 27.202765133500918 27.978887832053807 23.570266322440382 98-99 21.45281608804946 27.393335145050614 27.41069366125416 23.743155105645762 100-101 21.701562748404438 26.556141271042616 28.2134956729221 23.52880030763085 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1766.0 1 1208.5 2 723.5 3 771.0 4 628.5 5 630.5 6 1154.0 7 2284.5 8 3553.0 9 4113.0 10 3551.0 11 2904.0 12 2969.0 13 3183.0 14 3310.5 15 3522.0 16 3833.0 17 4016.0 18 4323.5 19 4861.5 20 5629.0 21 6692.0 22 8088.5 23 10038.5 24 12149.5 25 14868.0 26 19318.5 27 24255.0 28 27728.5 29 31605.0 30 36081.0 31 41404.5 32 48120.5 33 53818.5 34 59091.0 35 64165.0 36 70233.0 37 76103.5 38 79787.5 39 82355.5 40 85442.0 41 89183.5 42 90769.0 43 93351.5 44 97766.5 45 100347.0 46 104263.5 47 120032.5 48 152799.5 49 150312.0 50 118899.5 51 106053.5 52 98982.5 53 94423.5 54 90187.0 55 82190.0 56 73923.0 57 66960.0 58 60419.0 59 54243.5 60 46096.5 61 37465.0 62 29332.0 63 21350.0 64 14974.5 65 10863.5 66 8057.0 67 6277.0 68 4684.5 69 3509.5 70 2563.0 71 1480.5 72 962.5 73 666.5 74 397.0 75 272.5 76 161.5 77 79.5 78 40.5 79 27.0 80 15.5 81 8.5 82 6.0 83 2.5 84 1.5 85 1.0 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.40155581221550374 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 7.982879271689654E-4 16-17 0.002225015286364563 18-19 2.887424417419662E-4 20-21 0.0 22-23 0.0 24-25 1.3587879611386643E-4 26-27 1.6984849514233304E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011787485562877913 36-37 0.022912561994700726 38-39 0.009783273320198384 40-41 0.005486106393097358 42-43 0.004942591208641892 44-45 0.0016475304028806305 46-47 0.0012738637135674977 48-49 0.0029893335145050612 50-51 6.963788300835655E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 8.152727766831986E-4 60-61 0.0011040152184251647 62-63 0.0013587879611386643 64-65 8.832121747401317E-4 66-67 0.002734560771791562 68-69 0.002445818330049596 70-71 5.095454854269991E-5 72-73 0.0 74-75 0.0 76-77 0.09275426319722807 78-79 0.0039235002377878934 80-81 2.3778789319926625E-4 82-83 0.0 84-85 1.3587879611386643E-4 86-87 0.09280521774577077 88-89 0.44201372375840753 90-91 0.3927067056185883 92-93 0.0 94-95 0.0010360758203682315 96-97 0.0 98-99 0.0 100-101 4.076363883415993E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2943800.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.72704892343355 #Duplication Level Percentage of deduplicated Percentage of total 1 67.30222769859854 30.102300309312813 2 15.974598903920818 14.289933334157887 3 6.3810141345753815 8.562117941348223 4 3.261941206602772 5.835880157323441 5 1.9767336223318628 4.420673071731662 6 1.2206729598917097 3.2758259517953365 7 0.7733390751513219 2.4212422254087222 8 0.574241587851359 2.0547305274958316 9 0.42214007187187 1.6992971682439395 >10 1.952401213910797 14.991480278183374 >50 0.10729093504809818 3.3138014413209342 >100 0.04786482676010635 3.8685289471341706 >500 0.0026531742739197774 0.8125073318805106 >1k 0.0022741493774263914 2.178315746432867 >5k 4.548298754852783E-4 1.330377344600259 >10k+ 1.5160995849509276E-4 0.8429882236300584 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 13872 0.4712276649228888 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10991 0.3733609620218765 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9379 0.3186018071879883 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6977 0.23700659012161154 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6199 0.21057816427746448 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 6192 0.21034037638426525 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 5277 0.17925810177321827 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5214 0.1771180107344249 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4364 0.14824376656022828 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3959 0.1344860384536993 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3449 0.11716149194918132 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3231 0.10975609756097562 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3230 0.10972212786194714 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3229 0.10968815816291867 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 3.396969902846661E-5 0.0 0.0 0.0 5 0.0 3.396969902846661E-5 0.0 0.0 0.0 6 0.0 3.396969902846661E-5 0.0 0.0 0.0 7 0.0 6.793939805693321E-5 0.0 0.0 0.0 8 0.0 6.793939805693321E-5 0.0 0.0 0.0 9 0.0 6.793939805693321E-5 0.0 0.0 0.0 10-11 0.0 1.0190909708539982E-4 0.0 6.793939805693321E-5 0.0 12-13 0.0 1.0190909708539982E-4 0.0 6.793939805693321E-5 0.0 14-15 0.0 1.0190909708539982E-4 0.0 6.793939805693321E-5 0.0 16-17 0.0 1.0190909708539982E-4 0.0 1.0190909708539982E-4 0.0 18-19 0.0 1.0190909708539982E-4 0.0 1.6984849514233304E-4 0.0 20-21 0.0 1.3587879611386643E-4 0.0 2.3778789319926625E-4 0.0 22-23 0.0 1.3587879611386643E-4 0.0 4.925606359127658E-4 0.0 24-25 0.0 1.3587879611386643E-4 0.0 6.793939805693321E-4 0.0 26-27 0.0 1.3587879611386643E-4 0.0 0.0012908485630817312 0.0 28-29 0.0 1.3587879611386643E-4 0.0 0.00385556083973096 0.0 30-31 0.0 1.3587879611386643E-4 0.0 0.010632515795910048 0.0 32-33 0.0 1.3587879611386643E-4 0.0 0.024067531761668592 0.0 34-35 0.0 1.3587879611386643E-4 0.0 0.04512874515931789 0.0 36-37 0.0 1.3587879611386643E-4 0.0 0.07943814117806916 0.0 38-39 0.0 1.3587879611386643E-4 0.0 0.12962837149262857 0.0 40-41 0.0 1.3587879611386643E-4 0.0 0.18431958692845982 0.0 42-43 0.0 2.2080304368503296E-4 0.0 0.24101501460697058 0.0 44-45 0.0 3.057272912561995E-4 0.0 0.31131530674638225 0.0 46-47 0.0 3.057272912561995E-4 0.0 0.40113119097764793 0.0 48-49 0.0 3.566818397988994E-4 0.0 0.49554996942727086 0.0 50-51 0.0 3.736666893131327E-4 0.0 0.6038963244785651 0.0 52-53 0.0 4.416060873700659E-4 0.0 0.7117671037434609 0.0 54-55 0.0 5.095454854269991E-4 0.0 0.8334805353624567 0.0 56-57 0.0 5.265303349412324E-4 0.0 0.9794313472382635 0.0 58-59 0.0 6.284394320266323E-4 0.0 1.1415857055506489 0.0 60-61 0.0 6.454242815408655E-4 0.0 1.3244276105713704 0.0 62-63 0.0 6.454242815408655E-4 0.0 1.507286500441606 0.0 64-65 0.0 6.963788300835655E-4 0.0 1.6981112847340172 0.0 66-67 0.0 7.473333786262654E-4 0.0 1.917368707113255 0.0 68-69 0.0 7.473333786262654E-4 0.0 2.1795977987635027 0.0 70-71 0.0 7.473333786262654E-4 0.0 2.4650281948501935 0.0 72-73 0.0 7.473333786262654E-4 0.0 2.760785379441538 0.0 74-75 0.0 7.473333786262654E-4 0.0 3.0705890345811535 0.0 76-77 0.0 7.473333786262654E-4 0.0 3.3966641755554043 0.0 78-79 0.0 7.473333786262654E-4 0.0 3.7876723962225696 0.0 80-81 0.0 7.473333786262654E-4 0.0 4.211053740063863 0.0 82-83 0.0 7.473333786262654E-4 0.0 4.646494327060262 0.0 84-85 0.0 7.473333786262654E-4 0.0 5.101263672803858 0.0 86-87 0.0 7.813030776547319E-4 0.0 5.580304368503295 0.0 88-89 0.0 7.982879271689653E-4 0.0 6.094962293634078 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACT 10185 0.0 21.037693 12-13 TACATGG 12690 0.0 20.118357 2 GTACATG 13070 0.0 20.083887 1 GTAAGCG 300 0.0 19.772793 94-95 AGTACTT 11035 0.0 19.309692 12-13 TTTGCGT 670 0.0 19.12321 3 GTACTTT 11185 0.0 19.072273 14-15 ACATGGG 12835 0.0 18.929796 3 CATGGGG 5615 0.0 16.733519 4 TACTTTT 13100 0.0 16.10309 14-15 ACTTTTT 13080 0.0 16.090881 16-17 AGTACAT 9860 0.0 16.026518 2 CTTTGCG 885 0.0 15.549864 2 TATAGGG 950 0.0 14.985441 2 TACACTG 2350 0.0 14.740997 5 AGAGTAC 24395 0.0 14.696964 10-11 TCTATAC 940 0.0 14.640031 3 CATGGGA 6865 0.0 14.446999 4 GTATAGA 895 0.0 13.839555 1 CATGGGT 2095 0.0 13.817125 4 >>END_MODULE