FastQCFastQC Report
Fri 27 May 2016
SRR938968_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938968_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2962304
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT129680.4377673594607441No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116610.39364629693643866No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92420.3119868858834205No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT72910.24612598841982458No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60590.2045367389707471No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA59770.20176862334183124No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT57280.1933630039320745No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA49390.16672833038067666No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC46300.15629726051073758No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT37180.12551041351596595No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT35930.12129072505725275No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT30760.10383809359201486No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC30090.10157634057814457No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC29930.10103622045542929No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGT6400.022.1865793
GTACATG124000.021.8712021
TACATGG119850.021.7206952
GAGTACT98500.021.40777812-13
ACATGGG120050.020.8565563
AGTACTT105300.020.09274312-13
GTACTTT106250.019.77961314-15
CCTATAC7100.019.3325313
CTTTGCG7800.018.8111862
CATGGGG56650.018.2975644
CTATACT11200.017.7492644
TATCCCG2403.2002117E-617.7492645
CATGGGA61750.017.0162984
ACTTTTT131600.016.45629116-17
TACTTTT128750.016.43327114-15
GTATAGG7600.016.220271
GTATAGA9700.016.1302281
AGTACAT89400.015.7771232
TCTATAC8600.015.4102133
TTGCGTT9250.015.3507144
Produced by FastQC (version 0.11.5)