##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938968_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2962304 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.62736167523657 33.0 31.0 34.0 30.0 34.0 2 31.69471431696409 33.0 31.0 34.0 30.0 34.0 3 31.741101858553343 33.0 31.0 34.0 28.0 34.0 4 35.27832592468565 37.0 35.0 37.0 33.0 37.0 5 35.315621556734214 37.0 35.0 37.0 33.0 37.0 6 35.390193916627055 37.0 35.0 37.0 33.0 37.0 7 35.30278087596682 37.0 35.0 37.0 33.0 37.0 8 35.27886030603206 37.0 35.0 37.0 32.0 37.0 9 36.854926773214366 39.0 37.0 39.0 33.0 39.0 10-11 36.73281894768397 39.0 37.0 39.0 32.0 39.0 12-13 36.607272076059715 39.0 37.0 39.0 32.0 39.0 14-15 37.75726360292529 40.0 38.0 41.0 32.0 41.0 16-17 37.68582242065635 40.0 37.0 41.0 32.0 41.0 18-19 37.638922271313135 40.0 37.0 41.0 32.0 41.0 20-21 37.50672415795273 40.0 37.0 41.0 32.0 41.0 22-23 37.595667088860566 40.0 37.0 41.0 32.0 41.0 24-25 37.52932092722421 40.0 37.0 41.0 32.0 41.0 26-27 37.42799270432744 40.0 37.0 41.0 31.5 41.0 28-29 37.29589755136543 40.0 37.0 41.0 31.0 41.0 30-31 37.11127537889426 40.0 37.0 41.0 30.5 41.0 32-33 36.91078903448127 40.0 36.0 41.0 30.0 41.0 34-35 36.579705864084175 39.0 36.0 41.0 30.0 41.0 36-37 36.2987878691721 39.0 35.0 41.0 30.0 41.0 38-39 36.076820441116105 39.0 35.0 41.0 29.0 41.0 40-41 35.929288148684265 39.0 35.0 41.0 28.5 41.0 42-43 35.724110354642875 39.0 35.0 41.0 27.0 41.0 44-45 35.63748065694811 39.0 35.0 41.0 27.0 41.0 46-47 35.40472905549194 39.0 35.0 41.0 25.5 41.0 48-49 35.20323538705008 39.0 35.0 41.0 24.5 41.0 50-51 34.04878027373287 38.0 33.5 40.0 23.5 40.5 52-53 34.197015735049476 38.0 33.5 39.5 23.5 40.5 54-55 34.70290220044938 39.0 34.0 40.0 23.5 41.0 56-57 34.595455091712395 38.5 34.0 40.0 23.0 41.0 58-59 34.38603009684354 38.0 34.0 40.0 23.0 41.0 60-61 34.10829678520503 38.0 34.0 40.0 22.0 41.0 62-63 33.75456114564879 37.5 33.5 40.0 21.5 41.0 64-65 33.339257719666854 37.0 33.0 40.0 20.0 41.0 66-67 32.94319978638249 36.0 32.5 39.0 18.5 41.0 68-69 32.538521535939594 36.0 32.0 39.0 17.5 41.0 70-71 32.09993116844186 35.0 31.5 39.0 13.5 40.0 72-73 31.649838098993214 35.0 31.0 38.0 11.0 40.0 74-75 31.17886060984963 35.0 31.0 37.0 7.5 39.0 76-77 30.758602763254547 35.0 30.5 36.5 7.0 39.0 78-79 30.31095154312319 34.5 30.0 36.0 2.0 39.0 80-81 29.86007006708292 34.0 30.0 36.0 2.0 37.5 82-83 29.45481760143456 34.0 29.0 35.0 2.0 37.0 84-85 29.077978323629175 34.0 29.0 35.0 2.0 37.0 86-87 28.670883541999743 34.0 29.0 35.0 2.0 36.0 88-89 28.222716169576113 34.0 28.5 35.0 2.0 36.0 90-91 27.76251306415547 34.0 27.0 35.0 2.0 35.5 92-93 27.316074582487147 34.0 26.0 35.0 2.0 35.0 94-95 26.800544441083694 33.0 25.0 35.0 2.0 35.0 96-97 26.180220531046103 33.0 24.5 35.0 2.0 35.0 98-99 25.434107876841807 33.0 21.0 35.0 2.0 35.0 100-101 22.752178371969926 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.44037811329285503 1101 2 0.5139086292822306 1101 3 0.3752422538769018 1101 4 0.3271104147487094 1101 5 0.22443706797331942 1101 6 0.33417442966194955 1101 7 0.3328134135708041 1101 8 0.4096660512307295 1101 9 0.34305800895478455 1101 10-11 0.5250150148840973 1101 12-13 0.6297067045995064 1101 14-15 0.3943419685938423 1101 16-17 0.552947213257589 1101 18-19 0.6391181941528004 1101 20-21 0.7399313266949079 1101 22-23 0.544615400408702 1101 24-25 0.7340655513513425 1101 26-27 0.6502206729977829 1101 28-29 0.6087059296138619 1101 30-31 0.5684402912582058 1101 32-33 0.6529856795046527 1101 34-35 0.50164375740178 1101 36-37 0.489125647891548 1101 38-39 0.545525976909893 1101 40-41 0.5422017608607774 1101 42-43 0.44629913635199614 1101 44-45 0.5552212492677882 1101 46-47 0.5284831984983143 1101 48-49 0.5704890963199176 1101 50-51 0.4585974713463088 1101 52-53 0.5101832979486645 1101 54-55 0.511754039272077 1101 56-57 0.452791948462 1101 58-59 0.3979836308876017 1101 60-61 0.509773705099775 1101 62-63 0.457695573037995 1101 64-65 0.48333783674848974 1101 66-67 0.32815297741678506 1101 68-69 0.4353133400537459 1101 70-71 0.22540497300674645 1101 72-73 0.14929075263532354 1101 74-75 0.034565232912466826 1101 76-77 0.06721504525901523 1101 78-79 0.194273326864554 1101 80-81 0.08054983849207886 1101 82-83 0.1282627588615739 1101 84-85 -0.0519320354000854 1101 86-87 -0.1421578876384153 1101 88-89 -0.0749198383655596 1101 90-91 0.023511722915106503 1101 92-93 -0.003170692604243186 1101 94-95 -0.05861460772321436 1101 96-97 -0.01498271850119437 1101 98-99 0.0166199319631275 1101 100-101 0.36908338151665987 1102 1 0.28136893025621035 1102 2 0.2673485532027051 1102 3 0.2116960365549403 1102 4 0.25413812896177745 1102 5 0.2766129622544753 1102 6 0.28302712794256024 1102 7 0.22787881092452977 1102 8 0.23035248404530506 1102 9 0.3795441518482505 1102 10-11 0.22996167005958057 1102 12-13 0.246870496820506 1102 14-15 0.4486609016313068 1102 16-17 0.4695395835112848 1102 18-19 0.483379704318196 1102 20-21 0.5241605096655064 1102 22-23 0.45900859683851536 1102 24-25 0.49831065026463506 1102 26-27 0.5879371644900218 1102 28-29 0.6659224227571272 1102 30-31 0.6129033923574596 1102 32-33 0.5407295781181176 1102 34-35 0.38076101695381936 1102 36-37 0.5555969975436668 1102 38-39 0.5015689169345308 1102 40-41 0.672623335218276 1102 42-43 0.6348865469073743 1102 44-45 0.7321977815074803 1102 46-47 0.6558866874578726 1102 48-49 0.6974162627207718 1102 50-51 0.5675812284994066 1102 52-53 0.44132785895592264 1102 54-55 0.520558989724627 1102 56-57 0.595975170020516 1102 58-59 0.5396767966082265 1102 60-61 0.6074214806651739 1102 62-63 0.4730072382352475 1102 64-65 0.4099387593448327 1102 66-67 0.5147822647325668 1102 68-69 0.3674196268511096 1102 70-71 0.38591824564816335 1102 72-73 0.35072483850877134 1102 74-75 0.21613988759925107 1102 76-77 0.2645289822754684 1102 78-79 0.35899342751142527 1102 80-81 0.2559314744000325 1102 82-83 0.2137771046734045 1102 84-85 0.10328307748093124 1102 86-87 0.13612428023185075 1102 88-89 0.18109982211634446 1102 90-91 0.27316087874275397 1102 92-93 0.4481494721026493 1102 94-95 0.43210329374983303 1102 96-97 0.27121944699159073 1102 98-99 0.3331267787903407 1102 100-101 0.5035598889908783 1103 1 0.16114117071805723 1103 2 0.29986459101438 1103 3 0.39258898479176807 1103 4 0.36657339951732126 1103 5 0.3230298818558097 1103 6 0.24949247790393514 1103 7 0.24702647126808586 1103 8 0.28030420131022993 1103 9 0.4043417474828672 1103 10-11 0.4527453683272853 1103 12-13 0.535647297487678 1103 14-15 0.5297771853439812 1103 16-17 0.545705627380265 1103 18-19 0.571104021031374 1103 20-21 0.6712567735589445 1103 22-23 0.649043572237872 1103 24-25 0.7155036975507869 1103 26-27 0.6338683862552799 1103 28-29 0.6793128853879793 1103 30-31 0.6807668209916855 1103 32-33 0.6360129371999008 1103 34-35 0.7208164020897669 1103 36-37 0.6928930729926819 1103 38-39 0.6970056185148934 1103 40-41 0.6972369854656932 1103 42-43 0.6721467354314825 1103 44-45 0.7331356100755286 1103 46-47 0.7215003585602346 1103 48-49 0.6497888770252587 1103 50-51 0.7038511933107685 1103 52-53 0.7264224713244047 1103 54-55 0.8187781640511957 1103 56-57 0.7446234291824609 1103 58-59 0.8046060151262111 1103 60-61 0.8369093439016666 1103 62-63 0.6605120272592515 1103 64-65 0.6211623101656656 1103 66-67 0.6913498602101811 1103 68-69 0.6531927282242336 1103 70-71 0.7579674745889378 1103 72-73 0.7253905441291302 1103 74-75 0.585852775797246 1103 76-77 0.45137894915358245 1103 78-79 0.6268416367152092 1103 80-81 0.5448804456063705 1103 82-83 0.5812181501768059 1103 84-85 0.4997481275058746 1103 86-87 0.548783378346446 1103 88-89 0.544327639532856 1103 90-91 0.5869452305438116 1103 92-93 0.4866328388702996 1103 94-95 0.5070224339351981 1103 96-97 0.6097616762831457 1103 98-99 0.35437375980131947 1103 100-101 0.545782619438711 1104 1 0.23186445821968604 1104 2 0.17847315889379445 1104 3 0.23157176738736496 1104 4 0.25312494865154633 1104 5 0.2543307971856166 1104 6 0.2781959777424561 1104 7 0.23133580403452925 1104 8 0.31426759162220463 1104 9 0.5054948889977666 1104 10-11 0.3363125907250364 1104 12-13 0.35799079407905765 1104 14-15 0.5128461828613311 1104 16-17 0.37731595490282643 1104 18-19 0.4556482454247117 1104 20-21 0.3595677322286477 1104 22-23 0.4505479415608562 1104 24-25 0.48602713444395107 1104 26-27 0.4496130166572172 1104 28-29 0.4207571827250334 1104 30-31 0.465217661070902 1104 32-33 0.46357742873427554 1104 34-35 0.44824259840473246 1104 36-37 0.50738825748072 1104 38-39 0.5768367675274035 1104 40-41 0.6972236698786816 1104 42-43 0.55952437657524 1104 44-45 0.6418278851078156 1104 46-47 0.7535374272856004 1104 48-49 0.7415147581462662 1104 50-51 0.6873429023138016 1104 52-53 0.5998897041760713 1104 54-55 0.5538110062457164 1104 56-57 0.6468756906421831 1104 58-59 0.6946006363298878 1104 60-61 0.6909253658233183 1104 62-63 0.6338414905067751 1104 64-65 0.6482218606244388 1104 66-67 0.8161288827876945 1104 68-69 0.7201964116192912 1104 70-71 0.6279054344658022 1104 72-73 0.559617559703117 1104 74-75 0.5551679983748734 1104 76-77 0.5255029674940523 1104 78-79 0.6210672856894739 1104 80-81 0.5650067217564576 1104 82-83 0.4831721541679812 1104 84-85 0.647579993691231 1104 86-87 0.6234406041840579 1104 88-89 0.6662665875664437 1104 90-91 0.6532941141077657 1104 92-93 0.7078578261533295 1104 94-95 0.6009144924139846 1104 96-97 0.5611589076922812 1104 98-99 0.482878927475511 1104 100-101 0.8709657817621448 1105 1 0.13495134981342716 1105 2 0.20126464946127953 1105 3 0.15303947196805723 1105 4 0.18641600675484682 1105 5 0.2046516890330068 1105 6 0.1802606085711531 1105 7 0.11245260135451218 1105 8 0.17860820657826793 1105 9 0.3324192974587561 1105 10-11 0.31694048072260017 1105 12-13 0.3823773176497838 1105 14-15 0.42881936436945267 1105 16-17 0.48767150101310364 1105 18-19 0.42464050677247656 1105 20-21 0.39971126234746635 1105 22-23 0.3928861845337437 1105 24-25 0.41080716042608145 1105 26-27 0.383378071495045 1105 28-29 0.38360411097254143 1105 30-31 0.41226896377640543 1105 32-33 0.42130088474576155 1105 34-35 0.33812642150232364 1105 36-37 0.5055527126958239 1105 38-39 0.5145551276598113 1105 40-41 0.5268900840365518 1105 42-43 0.4025098304683894 1105 44-45 0.33104284551191654 1105 46-47 0.5165274057709368 1105 48-49 0.4729594741424137 1105 50-51 0.3729701810343897 1105 52-53 0.511553840041806 1105 54-55 0.5084977867812839 1105 56-57 0.5124372599444271 1105 58-59 0.5119281676735383 1105 60-61 0.5530842932664655 1105 62-63 0.44827431116725336 1105 64-65 0.4830705324395481 1105 66-67 0.4157558895757276 1105 68-69 0.42475967992874786 1105 70-71 0.26865967027246285 1105 72-73 0.44160745478055574 1105 74-75 0.4476646020742976 1105 76-77 0.3460620402725745 1105 78-79 0.43569285854740514 1105 80-81 0.2594566124692754 1105 82-83 0.3221410041788886 1105 84-85 0.3942377368566419 1105 86-87 0.5524299291171921 1105 88-89 0.5118811608665474 1105 90-91 0.5739544760522861 1105 92-93 0.6699307177904927 1105 94-95 0.534786377900673 1105 96-97 0.5002025962860834 1105 98-99 0.6889194294310244 1105 100-101 1.0218113434914073 1106 1 0.17429809290230125 1106 2 0.24272261352551538 1106 3 0.2980569024845572 1106 4 0.1653477878213394 1106 5 0.24694920097989836 1106 6 0.2587182362748095 1106 7 0.2159893956148622 1106 8 0.17431740966087972 1106 9 0.25300808413102516 1106 10-11 0.29865688070338337 1106 12-13 0.3025190899113426 1106 14-15 0.3854078695004759 1106 16-17 0.4650813688985167 1106 18-19 0.3479042736996334 1106 20-21 0.4782379217996393 1106 22-23 0.4197065415903154 1106 24-25 0.39760991438678417 1106 26-27 0.41184774591560114 1106 28-29 0.4448281656944175 1106 30-31 0.3591750893965937 1106 32-33 0.5480639860579757 1106 34-35 0.5041398244900392 1106 36-37 0.4223176819259393 1106 38-39 0.40429805884259906 1106 40-41 0.5968485160954344 1106 42-43 0.6097404323778619 1106 44-45 0.5878962768974034 1106 46-47 0.5729151774057968 1106 48-49 0.5037535558205093 1106 50-51 0.4230031403103993 1106 52-53 0.41611254105379203 1106 54-55 0.4058394553598603 1106 56-57 0.5017931734273375 1106 58-59 0.6681840683652709 1106 60-61 0.6564906378255699 1106 62-63 0.45558304754109713 1106 64-65 0.29043591545407565 1106 66-67 0.4496127475077074 1106 68-69 0.43135779536201113 1106 70-71 0.474751925113317 1106 72-73 0.48574395220439115 1106 74-75 0.5149488998556038 1106 76-77 0.5299756463625265 1106 78-79 0.6163196735917218 1106 80-81 0.5076556959712981 1106 82-83 0.5376811336201897 1106 84-85 0.7437524165933169 1106 86-87 0.7755962056191201 1106 88-89 0.8593458683851232 1106 90-91 0.6931997153995724 1106 92-93 0.7266922422009969 1106 94-95 0.6659546522626201 1106 96-97 0.7032259838364077 1106 98-99 0.5564537642661378 1106 100-101 1.07590753178836 1107 1 0.12380281216377753 1107 2 0.03848092959824001 1107 3 0.0664246412835432 1107 4 0.12896943800360816 1107 5 0.1386938638090598 1107 6 0.08191683472700362 1107 7 -0.01958863391133292 1107 8 0.17944724892954156 1107 9 0.1855977473755317 1107 10-11 0.12051190929403077 1107 12-13 0.16735133177502348 1107 14-15 0.18535622385294204 1107 16-17 0.2024755072366773 1107 18-19 0.15869123489288484 1107 20-21 0.18089193613377574 1107 22-23 0.1597464554711081 1107 24-25 0.29711929775749724 1107 26-27 0.2937291794925585 1107 28-29 0.31883968093754333 1107 30-31 0.4073107393963866 1107 32-33 0.29637962982079813 1107 34-35 0.1762221347833588 1107 36-37 0.15841523194085738 1107 38-39 0.2967452318476873 1107 40-41 0.17296728950960016 1107 42-43 0.4061175089278066 1107 44-45 0.4379087372184287 1107 46-47 0.6060286507577288 1107 48-49 0.43571305364913826 1107 50-51 0.47300256155232034 1107 52-53 0.3084519629221276 1107 54-55 0.38487888036031137 1107 56-57 0.42124639002857833 1107 58-59 0.4788976634606783 1107 60-61 0.3919352125473239 1107 62-63 0.26438691119385993 1107 64-65 0.48023469148287035 1107 66-67 0.3240935674649208 1107 68-69 0.41977735684042017 1107 70-71 0.551191568816229 1107 72-73 0.4058232488703375 1107 74-75 0.36317487016054883 1107 76-77 0.45319776962846703 1107 78-79 0.5406210104547995 1107 80-81 0.4852635458373662 1107 82-83 0.4874924244413563 1107 84-85 0.42855429748899 1107 86-87 0.4945509412900897 1107 88-89 0.5604726422786364 1107 90-91 0.7153283603839213 1107 92-93 0.50579577317 1107 94-95 0.6616172008953214 1107 96-97 0.631503899381304 1107 98-99 0.7696748798121256 1107 100-101 0.7882269762895433 1108 1 0.24871244200761922 1108 2 0.23842188776288253 1108 3 0.1699647415341694 1108 4 0.25095709946495504 1108 5 0.2819004379366845 1108 6 0.2801467846016905 1108 7 0.3115155689033955 1108 8 0.23080762101360364 1108 9 0.3399258753878698 1108 10-11 0.4280325136896366 1108 12-13 0.3501430466391042 1108 14-15 0.4152789874928118 1108 16-17 0.44261913553820165 1108 18-19 0.3695971053382294 1108 20-21 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-2.0947587862425934 2316 64-65 -2.585692901982007 2316 66-67 -2.45394414857639 2316 68-69 -2.614505870932433 2316 70-71 -2.540469013586687 2316 72-73 -2.504483214196288 2316 74-75 -2.6802367513626812 2316 76-77 -2.606252131005217 2316 78-79 -2.55612732521433 2316 80-81 -2.420070605125108 2316 82-83 -2.56679124691329 2316 84-85 -2.8433110878257324 2316 86-87 -2.856676421552219 2316 88-89 -3.086956311703549 2316 90-91 -3.264457597415067 2316 92-93 -3.475630588056635 2316 94-95 -3.6408131178002137 2316 96-97 -4.015984348698407 2316 98-99 -4.339127591218187 2316 100-101 -4.7106779561401595 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10593.0 3 2968.0 4 2922.0 5 3370.0 6 3895.0 7 4731.0 8 6092.0 9 7279.0 10 9277.0 11 11462.0 12 14599.0 13 19889.0 14 19062.0 15 18507.0 16 22406.0 17 28292.0 18 24905.0 19 19675.0 20 19719.0 21 21216.0 22 23501.0 23 25962.0 24 29077.0 25 33041.0 26 38439.0 27 44403.0 28 52188.0 29 60422.0 30 72043.0 31 86471.0 32 106216.0 33 134280.0 34 177847.0 35 252790.0 36 379789.0 37 569401.0 38 519648.0 39 85897.0 40 30.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.622816888820275 19.212732563886938 14.693453747225618 22.470996800067166 2 14.787589318174755 20.948499211391642 40.549708005422275 23.714203465011334 3 19.669384794052263 25.084194595529734 30.29309214049744 24.953328469920557 4 12.486220519007404 16.82205122486153 38.47623102892423 32.21549722720683 5 13.299556510222436 37.96131233501778 34.55293303787625 14.186198116883533 6 30.610459056371926 36.73765258266721 17.885903353245787 14.765985007715077 7 27.277003189397643 33.50888141989185 21.382040775227377 17.832074615483126 8 25.18023605288704 34.86721644984054 20.397581963183427 19.554965534089 9 25.880199118483066 17.59769740532343 21.290857336715952 35.23124613947755 10-11 24.550650358143404 26.54445804783253 27.719280581057447 21.185611012966618 12-13 26.10152562468977 24.194739735543973 27.867865919650857 21.835868720115396 14-15 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26.91426731288843 29.46489789176685 21.59628047328258 40-41 22.022536239925905 26.976833530004484 29.42824862898658 21.57238160108303 42-43 21.620292165468094 27.19049416646751 28.846383644382644 22.34283002368175 44-45 21.98056761734732 27.01081155688762 28.785466746776166 22.223154078988898 46-47 22.407830375808132 27.294617720027738 28.321013494530977 21.976538409633157 48-49 22.150267688748922 27.15264812529586 28.55809046202202 22.1389937239332 50-51 21.90180651483517 27.431739151250756 28.820028637757495 21.846425696156587 52-53 22.15840358712418 27.69262243939045 28.142795658031343 22.006178315454022 54-55 21.81422321666545 27.3326927549567 28.424873228294324 22.428210800083523 56-57 22.164978198014843 27.536555578260064 27.99650855956188 22.30195766416321 58-59 22.414173194404537 27.18781539466219 28.16310245418732 22.234908956745947 60-61 22.076282484902066 27.18915825404698 28.809374866423727 21.92518439462723 62-63 22.016096438077874 27.17109505718525 28.722498636506455 22.090309868230424 64-65 21.861756338533755 27.698535846842105 28.408096397129768 22.031611417494375 66-67 21.87893147982599 27.35730851060939 28.116347347855974 22.647412661708643 68-69 21.742959689934594 27.44634874614193 28.3680399761833 22.442651587740176 70-71 22.253293805795753 27.16606269825541 27.98898133378438 22.591662162164457 72-73 21.93417480604813 26.87582750165946 28.468213662817597 22.721784029474808 74-75 21.596101990776273 27.842340843176277 28.004706953113857 22.556850212933593 76-77 21.939059798964433 27.101737796730855 28.056763702903613 22.902438701401095 78-79 21.956360046589296 27.159804612829163 28.28043945479253 22.60339588578901 80-81 22.019695236414123 27.271554606587483 27.995376609445703 22.713373547552692 82-83 21.908912267931616 27.095269750002377 28.34262670651998 22.653191275546025 84-85 21.821999764700408 27.20197380025645 28.039035964337 22.936990470706135 86-87 21.7025870044438 27.25762386974092 28.16369841922448 22.8760907065908 88-89 21.939206612732 26.9628449769134 27.953742929160626 23.144205481193975 90-91 21.772330851528604 26.67675485283128 28.256220458563075 23.294693837077038 92-93 21.91575513614161 27.065659914704003 28.094492949028727 22.924092000125658 94-95 21.841908283601654 26.89764330941956 28.054541601403038 23.205906805575744 96-97 21.337450048071354 26.696243030842083 28.432500243667945 23.53380667741862 98-99 21.617329537494662 26.84006619227174 27.879223726652068 23.663380543581532 100-101 21.81596552271506 26.09535580378099 28.39486575708522 23.69381291641873 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4359.0 1 2540.0 2 833.0 3 935.5 4 799.5 5 839.5 6 1416.0 7 2658.0 8 4126.0 9 4830.0 10 4355.5 11 3740.5 12 3804.0 13 4092.0 14 4364.0 15 4632.5 16 5010.0 17 5267.5 18 5476.0 19 5795.5 20 6261.5 21 7079.0 22 8448.5 23 10446.5 24 12447.0 25 14938.5 26 19280.0 27 24060.0 28 27384.5 29 31536.5 30 36319.0 31 41417.5 32 48025.5 33 53655.0 34 58865.5 35 64140.0 36 70201.0 37 76234.0 38 79859.0 39 82008.5 40 84569.0 41 88258.5 42 90485.5 43 92840.0 44 97105.0 45 99638.5 46 103683.0 47 118137.5 48 150208.0 49 149586.5 50 118517.5 51 105636.5 52 99419.5 53 95092.0 54 90391.5 55 82478.0 56 74568.5 57 67713.0 58 61099.5 59 55026.0 60 47088.5 61 38602.0 62 30220.0 63 22149.0 64 15831.0 65 11549.5 66 8584.0 67 6573.0 68 4949.0 69 3821.0 70 2868.0 71 1763.0 72 1154.5 73 786.5 74 514.0 75 366.5 76 227.5 77 128.0 78 84.0 79 56.0 80 43.0 81 34.0 82 31.5 83 27.0 84 18.5 85 14.0 86 13.0 87 12.5 88 10.0 89 7.5 90 5.0 91 3.5 92 3.5 93 2.5 94 2.5 95 2.0 96 1.5 97 1.5 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28666875513114115 2 0.08979497040141728 3 0.09421720390614872 4 0.10782147949704014 5 0.11048832260294689 6 0.08490013178930994 7 0.09489235405954284 8 0.09158411830791167 9 0.09654647193535842 10-11 0.08998063669360067 12-13 0.09435223393682755 14-15 0.10329797346929957 16-17 0.09163475456941624 18-19 0.10279161085425398 20-21 0.1046820312837575 22-23 0.11833694313615349 24-25 0.13143485611199932 26-27 0.1339835479410621 28-29 0.12562856479280993 30-31 0.11789809553644731 32-33 0.11251377306312925 34-35 0.3829958032666465 36-37 0.5851357591928446 38-39 0.5696748206801192 40-41 0.5320520783822322 42-43 0.5595644471330424 44-45 0.579565770427343 46-47 0.5688646404960463 48-49 0.5896086289590805 50-51 0.5820131897333967 52-53 0.5848994566391565 54-55 0.5755317482608132 56-57 0.5482894395713607 58-59 0.543310207190079 60-61 0.4911717365942186 62-63 0.5652863446830575 64-65 0.5986893985222314 66-67 0.611686038975068 68-69 0.612732521712829 70-71 0.6085634695156203 72-73 0.6016769379510003 74-75 0.5983180659378645 76-77 0.5824182798254331 78-79 0.5591255995333363 80-81 0.5540957308905501 82-83 0.5683413991271659 84-85 0.5780129250745366 86-87 0.5688477617422114 88-89 0.5805447381497645 90-91 0.5943178012790045 92-93 0.602622148165752 94-95 0.6020989067968716 96-97 0.5982843084301949 98-99 0.602622148165752 100-101 0.5798358304887007 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2962304.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.9181616906772 #Duplication Level Percentage of deduplicated Percentage of total 1 69.69270021744295 32.698533774618824 2 14.866820109742715 13.950477394702402 3 5.942338678680273 8.364108208412585 4 3.0596278364348923 5.742084541725966 5 1.7775400280986853 4.16994552249925 6 1.0822498174934465 3.046630315611804 7 0.7409966532442475 2.433634055241499 8 0.5309245941025175 1.992800476132726 9 0.3776767239733153 1.5947907841966744 >10 1.7825710993068993 14.261420130451993 >50 0.09910560945792497 3.173854844319521 >100 0.04255713638318154 3.5868115652403243 >500 0.0021580127820928306 0.708986198433124 >1k 0.0022299465410803273 2.2871698333338544 >5k 3.5966879694843997E-4 1.15752366565121 >10k+ 1.4386751877937598E-4 0.8312286894283365 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 12968 0.4377673594607441 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11661 0.39364629693643866 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9242 0.3119868858834205 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7291 0.24612598841982458 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6059 0.2045367389707471 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5977 0.20176862334183124 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5728 0.1933630039320745 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4939 0.16672833038067666 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4630 0.15629726051073758 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3718 0.12551041351596595 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3593 0.12129072505725275 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3076 0.10383809359201486 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3009 0.10157634057814457 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2993 0.10103622045542929 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 3.375750766970574E-5 0.0 8 0.0 0.0 0.0 3.375750766970574E-5 0.0 9 0.0 0.0 0.0 3.375750766970574E-5 0.0 10-11 0.0 1.0127252300911723E-4 0.0 1.0127252300911723E-4 0.0 12-13 0.0 1.3503003067882297E-4 0.0 1.181512768439701E-4 0.0 14-15 0.0 1.3503003067882297E-4 0.0 1.687875383485287E-4 0.0 16-17 0.0 1.3503003067882297E-4 0.0 1.8566629218338157E-4 0.0 18-19 0.0 1.3503003067882297E-4 0.0 2.5318130752279304E-4 0.0 20-21 0.0 2.7006006135764593E-4 0.0 3.544538305319103E-4 0.0 22-23 0.0 2.7006006135764593E-4 0.0 4.726051073758804E-4 0.0 24-25 0.0 3.2069632286220456E-4 0.0 7.764226764032322E-4 0.0 26-27 0.0 3.713325843667632E-4 0.0 0.0015190878451367584 0.0 28-29 0.0 4.050900920364689E-4 0.0 0.00423656721254807 0.0 30-31 0.0 4.2196884587132177E-4 0.0 0.011933278961240981 0.0 32-33 0.0 4.5572635354102756E-4 0.0 0.026297098474700773 0.0 34-35 0.0 4.726051073758804E-4 0.0 0.04719299572224863 0.0 36-37 0.0 4.726051073758804E-4 0.0 0.08208137989888951 0.0 38-39 0.0 5.063626150455862E-4 0.0 0.13192433997321004 0.0 40-41 0.0 5.063626150455862E-4 0.0 0.18964967808840688 0.0 42-43 0.0 5.23241368880439E-4 0.0 0.24904601283325412 0.0 44-45 0.0 5.401201227152919E-4 0.0 0.3228905608607354 0.0 46-47 0.0 5.738776303849977E-4 0.0 0.4135969839692347 0.0 48-49 0.0 6.413926457244091E-4 0.0 0.5114937562113815 0.0 50-51 0.0 6.413926457244091E-4 0.0 0.6198215983234672 0.0 52-53 0.0 6.751501533941149E-4 0.0 0.7272886239899754 0.0 54-55 0.0 8.101801840729379E-4 0.0 0.848241773970531 0.0 56-57 0.0 8.101801840729379E-4 0.0 0.9945299334572009 0.0 58-59 0.0 8.776951994123493E-4 0.0 1.156160880179752 0.0 60-61 0.0 8.776951994123493E-4 0.0 1.3378100289504387 0.0 62-63 0.0 9.452102147517608E-4 0.0 1.5244552888562417 0.0 64-65 0.0 9.958464762563194E-4 0.0 1.721953587477855 0.0 66-67 0.0 0.0010296039839260253 0.0 1.9441455029598584 0.0 68-69 0.0 0.001046482737760878 0.0 2.205546763600225 0.0 70-71 0.0 0.001046482737760878 0.0 2.496249540897896 0.0 72-73 0.0 0.0010633614915957308 0.0 2.790260553947198 0.0 74-75 0.0 0.0010802402454305837 0.0 3.099614354232381 0.0 76-77 0.0 0.0010802402454305837 0.0 3.4228256114159787 0.0 78-79 0.0 0.0010802402454305837 0.0 3.81157706974031 0.0 80-81 0.0 0.0010802402454305837 0.0 4.235706396102493 0.0 82-83 0.0 0.0010802402454305837 0.0 4.663380260769996 0.0 84-85 0.0 0.0010802402454305837 0.0 5.112169446484899 0.0 86-87 0.0 0.0010802402454305837 0.0 5.590023846303417 0.0 88-89 0.0 0.0010802402454305837 0.0 6.104319475651385 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTGCGT 640 0.0 22.186579 3 GTACATG 12400 0.0 21.871202 1 TACATGG 11985 0.0 21.720695 2 GAGTACT 9850 0.0 21.407778 12-13 ACATGGG 12005 0.0 20.856556 3 AGTACTT 10530 0.0 20.092743 12-13 GTACTTT 10625 0.0 19.779613 14-15 CCTATAC 710 0.0 19.332531 3 CTTTGCG 780 0.0 18.811186 2 CATGGGG 5665 0.0 18.297564 4 CTATACT 1120 0.0 17.749264 4 TATCCCG 240 3.2002117E-6 17.749264 5 CATGGGA 6175 0.0 17.016298 4 ACTTTTT 13160 0.0 16.456291 16-17 TACTTTT 12875 0.0 16.433271 14-15 GTATAGG 760 0.0 16.22027 1 GTATAGA 970 0.0 16.130228 1 AGTACAT 8940 0.0 15.777123 2 TCTATAC 860 0.0 15.410213 3 TTGCGTT 925 0.0 15.350714 4 >>END_MODULE