FastQCFastQC Report
Fri 27 May 2016
SRR938968_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938968_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2962304
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT138120.4662586959339757No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111550.37656499805556753No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94280.31826578230998576No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT68180.23015868729205374No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA63020.2127398133344856No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61750.20845260986043299No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA52810.17827339800371603No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT51640.17432376960636045No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC45320.15298902475910642No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT40570.1369542086159962No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC35010.1181850343516398No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT32820.11079214017197425No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT31700.10701129931296721No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC31650.10684251177461868No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA30340.10242027826988721No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG29860.10079991790174134No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT103250.021.3021212-13
TTTGCGT7300.020.1505683
GTACATG130500.020.1178651
TACATGG125750.020.1125762
GTACTTT109550.019.83883914-15
AGTACTT111350.019.21982612-13
ACATGGG126350.018.8904043
AGCCGCG4400.018.3334717
CATGGGG56250.018.1369574
CTTTGCG8500.017.8640332
GCCGCGC4850.017.6108068
ACTTTTT131000.016.55377416-17
TACTTTT128600.016.30964314-15
TAGACCG2355.184034E-516.1536485
TTGCGTT9600.015.8171134
GCACCGT5103.6379788E-1215.8171126
GTATAGG6850.015.302871
AGTACAT99850.015.3022842
TGCACCG5004.1836756E-1115.1844285
TATACTG14700.015.1715165
Produced by FastQC (version 0.11.5)