##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938967_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 963413 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.784834748960208 33.0 31.0 34.0 30.0 34.0 2 31.814990040616017 33.0 31.0 34.0 30.0 34.0 3 31.82891241866157 33.0 31.0 34.0 30.0 34.0 4 35.36375780688033 37.0 35.0 37.0 33.0 37.0 5 35.43922596020606 37.0 35.0 37.0 33.0 37.0 6 35.572068261482876 37.0 35.0 37.0 33.0 37.0 7 35.52037495861069 37.0 35.0 37.0 33.0 37.0 8 35.531360901295706 37.0 35.0 37.0 33.0 37.0 9 37.07237705947501 39.0 37.0 39.0 33.0 39.0 10-11 36.96748123598083 39.0 37.0 39.0 33.0 39.0 12-13 36.87768641278455 39.0 37.0 39.0 33.0 39.0 14-15 37.89864004326286 40.0 38.0 41.0 33.0 41.0 16-17 37.85071355690654 40.0 37.5 41.0 32.5 41.0 18-19 37.91760127795659 40.0 38.0 41.0 33.0 41.0 20-21 38.03142785077635 40.0 38.0 41.0 33.0 41.0 22-23 37.98341157945761 40.0 38.0 41.0 33.0 41.0 24-25 37.870212982386576 40.0 37.5 41.0 33.0 41.0 26-27 37.643188331484005 40.0 37.0 41.0 32.5 41.0 28-29 37.497344856255836 40.0 37.0 41.0 32.0 41.0 30-31 37.32175193816151 40.0 36.5 41.0 32.0 41.0 32-33 37.047704359397265 39.5 35.5 41.0 31.0 41.0 34-35 36.701264151511346 39.0 35.0 41.0 30.5 41.0 36-37 36.34164942760789 39.0 35.0 41.0 30.0 41.0 38-39 35.99356506503442 38.5 35.0 40.5 29.5 41.0 40-41 35.82868198789097 39.0 35.0 40.0 29.5 41.0 42-43 35.63123810868236 38.5 35.0 40.0 28.5 41.0 44-45 35.45312186985228 39.0 35.0 41.0 26.5 41.0 46-47 35.03970208000099 39.0 35.0 41.0 24.0 41.0 48-49 34.66266180755294 39.0 35.0 41.0 23.0 41.0 50-51 33.48353302270158 38.0 33.0 40.0 20.5 40.5 52-53 33.543373402684 37.5 33.5 39.5 20.0 40.5 54-55 34.069575042063995 38.0 34.0 40.0 20.0 41.0 56-57 34.028909719922815 38.0 34.0 40.0 19.5 41.0 58-59 33.861109928971274 38.0 34.0 40.0 18.5 41.0 60-61 33.48262219837183 38.0 33.5 40.0 14.0 41.0 62-63 33.06686384759185 37.0 33.0 40.0 11.5 41.0 64-65 32.5835270024382 36.5 32.0 39.5 8.0 41.0 66-67 32.11197222790226 36.0 32.0 39.0 6.0 41.0 68-69 31.679389316938842 35.5 31.5 39.0 2.0 40.0 70-71 31.11076558028592 35.0 31.0 37.5 2.0 40.0 72-73 30.59437541324437 35.0 30.5 37.0 2.0 39.0 74-75 30.128614104231517 35.0 30.0 37.0 2.0 39.0 76-77 29.62087754680495 34.5 29.5 36.0 2.0 38.5 78-79 29.202317178613946 34.0 29.0 36.0 2.0 37.5 80-81 28.7715175111816 34.0 29.0 35.0 2.0 37.0 82-83 28.295404463091117 34.0 28.5 35.0 2.0 36.5 84-85 27.869681019458945 34.0 27.0 35.0 2.0 36.0 86-87 27.42022476341922 34.0 26.0 35.0 2.0 36.0 88-89 26.82301204156473 33.5 25.5 35.0 2.0 35.0 90-91 26.250316323321357 33.0 24.5 35.0 2.0 35.0 92-93 25.775762315850002 33.0 21.5 35.0 2.0 35.0 94-95 25.21880076353547 32.5 19.0 35.0 2.0 35.0 96-97 24.49765105930686 32.0 6.5 35.0 2.0 35.0 98-99 23.64527155020744 32.0 2.0 34.5 2.0 35.0 100-101 20.643304065857528 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19377389444867887 1101 2 0.2953820854922391 1101 3 0.22291560939674682 1101 4 0.15099117187379107 1101 5 0.14710168552529268 1101 6 0.25524321746689793 1101 7 0.26846371944373715 1101 8 0.36684313183582873 1101 9 0.427991706372012 1101 10-11 0.26490243749599784 1101 12-13 0.3635337814955122 1101 14-15 0.3986975047175676 1101 16-17 0.46975282988295675 1101 18-19 0.47722994294630894 1101 20-21 0.39427442021383996 1101 22-23 0.42087045393081013 1101 24-25 0.46427124763039274 1101 26-27 0.39685234633972755 1101 28-29 0.6398352033180785 1101 30-31 0.49172645061188547 1101 32-33 0.4631274086720367 1101 34-35 0.19328827776511304 1101 36-37 0.41183161708833893 1101 38-39 0.4503002463533079 1101 40-41 0.45193446677296834 1101 42-43 0.5329596267212438 1101 44-45 0.6583945260601141 1101 46-47 0.6567192292359465 1101 48-49 0.6453770830411898 1101 50-51 0.6754261089965112 1101 52-53 0.5178193532462814 1101 54-55 0.4065840449135152 1101 56-57 0.44650330251963766 1101 58-59 0.48234805212928933 1101 60-61 0.5653472015035987 1101 62-63 0.4272218056121915 1101 64-65 0.16095212562384376 1101 66-67 0.3108651759492389 1101 68-69 0.06827042801992178 1101 70-71 0.03582275721759842 1101 72-73 0.030414158602802388 1101 74-75 -0.15914316815967666 1101 76-77 -0.09927001938574165 1101 78-79 -0.09111553060927946 1101 80-81 -0.03748606405203603 1101 82-83 0.10295771224036443 1101 84-85 0.24328308778202867 1101 86-87 0.18779316148972214 1101 88-89 0.24416781195841963 1101 90-91 0.158761620893614 1101 92-93 0.15176930739747974 1101 94-95 0.09048926008793146 1101 96-97 -0.21714483236424442 1101 98-99 -0.29547417937045495 1101 100-101 0.08300872801273584 1102 1 0.27136999989727784 1102 2 0.40948174574139173 1102 3 0.4211691432250255 1102 4 0.09425985817050986 1102 5 0.27933974181487997 1102 6 0.20814866396662524 1102 7 0.35020184913628327 1102 8 0.32235045115967864 1102 9 0.46865694692400695 1102 10-11 0.3538268481750748 1102 12-13 0.2745536632118828 1102 14-15 0.3381865938916704 1102 16-17 0.3327350611232589 1102 18-19 0.4510381934391532 1102 20-21 0.4607873338919575 1102 22-23 0.4398424978886979 1102 24-25 0.5198438431591086 1102 26-27 0.5517031023902277 1102 28-29 0.20418535117261882 1102 30-31 0.3498673633252736 1102 32-33 0.3641756291745324 1102 34-35 0.38927483978013555 1102 36-37 0.235131027767423 1102 38-39 0.5066828959904797 1102 40-41 0.4912089833620428 1102 42-43 0.4203758686993453 1102 44-45 0.5393388551570482 1102 46-47 0.44185499849898235 1102 48-49 0.44882099269263875 1102 50-51 0.4375375976085607 1102 52-53 0.3369288966798649 1102 54-55 0.5122567830536937 1102 56-57 0.44666518226461704 1102 58-59 0.31875972215151194 1102 60-61 0.43328544339781416 1102 62-63 0.30326124397468135 1102 64-65 0.28706300079686287 1102 66-67 0.23647324083745502 1102 68-69 0.20631649362119475 1102 70-71 -0.014491040441900083 1102 72-73 0.1491126454531866 1102 74-75 0.08020357170022763 1102 76-77 0.0880812085409417 1102 78-79 -0.09936484376728316 1102 80-81 0.15236348609267125 1102 82-83 0.058795344890004486 1102 84-85 0.1573819615708807 1102 86-87 0.1403414239367642 1102 88-89 0.3351003179355203 1102 90-91 0.34413293501634 1102 92-93 0.28916457710093013 1102 94-95 0.07183835408566708 1102 96-97 0.1528001940608199 1102 98-99 0.1280419011211329 1102 100-101 0.07517099574479147 1103 1 0.1379170985923217 1103 2 0.1868477662659096 1103 3 0.21311823313591916 1103 4 0.24124531997210852 1103 5 -0.015322722816229373 1103 6 0.10490185244586314 1103 7 0.17602721127151 1103 8 0.07327954444909324 1103 9 0.3119146858487909 1103 10-11 0.21761108209543067 1103 12-13 0.15557165383648197 1103 14-15 0.2316094316381978 1103 16-17 0.35982063800572206 1103 18-19 0.2442119972315524 1103 20-21 0.21146237823504777 1103 22-23 0.10975307265959344 1103 24-25 0.2404573086770796 1103 26-27 0.2975452779466323 1103 28-29 0.1785791969853605 1103 30-31 0.17363261945940422 1103 32-33 0.24837220103145086 1103 34-35 0.25768210465618324 1103 36-37 0.2818720746866319 1103 38-39 0.29266282546385725 1103 40-41 0.20417590552936815 1103 42-43 0.33457793065297636 1103 44-45 0.43072336252645016 1103 46-47 0.496942708072865 1103 48-49 0.46231000194133287 1103 50-51 0.3431501657068807 1103 52-53 0.5172950702936134 1103 54-55 0.6489958239760298 1103 56-57 0.40151375589525884 1103 58-59 0.42488289563857506 1103 60-61 0.5424756805277653 1103 62-63 0.07730813151216154 1103 64-65 -0.06873287846324416 1103 66-67 0.010700272173238545 1103 68-69 0.08955295842122979 1103 70-71 0.16822228148892648 1103 72-73 0.1472498398026083 1103 74-75 0.16632484476919274 1103 76-77 0.4004736501558739 1103 78-79 0.08380708600170905 1103 80-81 0.021517720857652023 1103 82-83 0.05255035988218992 1103 84-85 -0.034326672661986635 1103 86-87 -0.007249136264253053 1103 88-89 -0.12110890225958926 1103 90-91 0.006235958792682794 1103 92-93 -0.0347663057488532 1103 94-95 -0.04158097316632947 1103 96-97 -0.07696629206612471 1103 98-99 -0.2177266332342036 1103 100-101 0.03264980080412627 1104 1 0.042524690140567145 1104 2 0.217043822490826 1104 3 0.11546205351680783 1104 4 0.2902954112179117 1104 5 0.11575078604195 1104 6 0.17445844157718682 1104 7 0.18441564814974498 1104 8 0.28583594568355153 1104 9 0.21173862905760643 1104 10-11 0.315960292285105 1104 12-13 0.1865488977240446 1104 14-15 0.3298691977709467 1104 16-17 0.2558197216867555 1104 18-19 0.26975631901394337 1104 20-21 0.2957000188681249 1104 22-23 0.16373159579020324 1104 24-25 0.31855162260898595 1104 26-27 0.15678028030178126 1104 28-29 0.19684508907078424 1104 30-31 0.23493654650393836 1104 32-33 0.15829688784855733 1104 34-35 0.09313399662320165 1104 36-37 0.13218969761107502 1104 38-39 0.3008916787009639 1104 40-41 0.19419420616949878 1104 42-43 -0.033078326184025286 1104 44-45 0.049572635777401786 1104 46-47 0.2795318754323475 1104 48-49 0.557369086497495 1104 50-51 0.1934317536155845 1104 52-53 0.20487341427872252 1104 54-55 0.38903600301857466 1104 56-57 0.47977408829253676 1104 58-59 0.3699874100538665 1104 60-61 0.16554941589175343 1104 62-63 0.3154127154235624 1104 64-65 0.23851067448635632 1104 66-67 0.2573914855494337 1104 68-69 0.31394493187886496 1104 70-71 0.3371126089185239 1104 72-73 0.30639333895707566 1104 74-75 0.247778136953972 1104 76-77 0.1810487175284834 1104 78-79 0.13354184254657042 1104 80-81 0.05295055030135032 1104 82-83 0.06977895830025105 1104 84-85 0.12249514190697042 1104 86-87 0.22789028063771255 1104 88-89 0.19470261916451292 1104 90-91 0.367714784360043 1104 92-93 0.02033123331696629 1104 94-95 0.08531183038363466 1104 96-97 0.21968554018572561 1104 98-99 0.10904918521109153 1104 100-101 0.12683761015830086 1105 1 -0.10357307842695107 1105 2 -0.07986251655368903 1105 3 -0.012247675232774924 1105 4 0.1785153226513856 1105 5 0.05862784138986399 1105 6 0.11483885825927587 1105 7 0.056102729524873496 1105 8 0.20665524775956357 1105 9 0.2224517253089502 1105 10-11 0.18060090707004406 1105 12-13 0.163943462847584 1105 14-15 0.36921839413454194 1105 16-17 0.19582514916211125 1105 18-19 0.25526557655998516 1105 20-21 0.26994603152401453 1105 22-23 0.0720905246039294 1105 24-25 0.17540319349304667 1105 26-27 0.1447491709998232 1105 28-29 0.13358539670995384 1105 30-31 0.14272559040481525 1105 32-33 0.2747649582577125 1105 34-35 0.1395271925427295 1105 36-37 0.19474320141647894 1105 38-39 0.029341517726976463 1105 40-41 0.00368241991334628 1105 42-43 -0.09046771053561997 1105 44-45 -0.0038343385284420606 1105 46-47 -0.026710954749347593 1105 48-49 0.18537609287478318 1105 50-51 0.05921337174335406 1105 52-53 0.014124175975553044 1105 54-55 -0.25244039363118986 1105 56-57 -0.15921862162124256 1105 58-59 -0.0473965468223696 1105 60-61 0.013592440969155462 1105 62-63 -0.1019262180775371 1105 64-65 -0.0039149135929363865 1105 66-67 -0.23306417567741988 1105 68-69 -0.2458047874959881 1105 70-71 -0.2744978710429926 1105 72-73 -0.28559250026630423 1105 74-75 0.0013423846935509687 1105 76-77 0.09311498206856328 1105 78-79 0.20250408458729652 1105 80-81 0.11189786678484026 1105 82-83 0.06044986826583809 1105 84-85 0.00828132404810944 1105 86-87 -0.020464207514354626 1105 88-89 0.19446393360036396 1105 90-91 0.15484179961865152 1105 92-93 0.09711274042819085 1105 94-95 -0.37245809400176455 1105 96-97 -0.31094639063863383 1105 98-99 0.010917963659078822 1105 100-101 0.16538995445760563 1106 1 0.005140411682862833 1106 2 0.2446787638457586 1106 3 0.16561506678436544 1106 4 0.1249773768073581 1106 5 0.18739056420276512 1106 6 0.1348252407031012 1106 7 0.14488221240690535 1106 8 0.08489334549171446 1106 9 0.14484911353150665 1106 10-11 0.13898356039848636 1106 12-13 0.13979150488741965 1106 14-15 0.22901866997734288 1106 16-17 0.19978296965479103 1106 18-19 0.2539387377534368 1106 20-21 0.23297960403750295 1106 22-23 0.3157225712527918 1106 24-25 0.28197628310704204 1106 26-27 0.250627416080917 1106 28-29 0.05343211191098618 1106 30-31 0.3123200316485466 1106 32-33 0.33214724495964987 1106 34-35 0.3673192280182107 1106 36-37 0.26301604137528045 1106 38-39 0.2541499117282626 1106 40-41 0.22029060092209818 1106 42-43 0.30451125208593055 1106 44-45 0.5606639941938525 1106 46-47 0.6835109415826395 1106 48-49 0.5535756075311085 1106 50-51 0.378077178723224 1106 52-53 0.3910441641038318 1106 54-55 0.356650198034167 1106 56-57 0.46629858363723287 1106 58-59 0.5450439636613993 1106 60-61 0.7414682767903145 1106 62-63 0.5958338685161522 1106 64-65 0.32909020171982917 1106 66-67 0.4698461404942975 1106 68-69 0.6598168190264744 1106 70-71 0.7499857966188053 1106 72-73 0.6283846990563084 1106 74-75 0.6822865352548355 1106 76-77 0.5817006766512236 1106 78-79 0.5154163211836078 1106 80-81 0.3940379181125486 1106 82-83 0.7179037344297008 1106 84-85 0.6829299736404586 1106 86-87 0.3940262689255256 1106 88-89 0.20147522918862393 1106 90-91 0.11773221320827076 1106 92-93 0.35608897953851226 1106 94-95 0.46610752341473827 1106 96-97 0.5996019778733874 1106 98-99 0.5507562988255472 1106 100-101 0.7257541367931886 1107 1 -0.06908433155281912 1107 2 0.13405801692783115 1107 3 0.15496543952132313 1107 4 0.23885731579763103 1107 5 -0.055366786747583774 1107 6 0.07265352900115118 1107 7 0.10940225717143903 1107 8 0.1778903130554781 1107 9 0.21026083895162628 1107 10-11 0.19868521289573238 1107 12-13 0.04050234494446414 1107 14-15 0.2260652034334143 1107 16-17 0.13157176859343878 1107 18-19 0.2617922737906966 1107 20-21 0.48465150526671863 1107 22-23 0.3896816378761372 1107 24-25 0.3090583754880498 1107 26-27 0.22826658324396476 1107 28-29 0.4571573379899476 1107 30-31 0.38721827355754357 1107 32-33 0.5588652870527469 1107 34-35 0.4150614844756788 1107 36-37 0.5399999857084126 1107 38-39 0.540365413253511 1107 40-41 0.40326563747377975 1107 42-43 0.4690164054225079 1107 44-45 0.19445106482434227 1107 46-47 0.3251628296352038 1107 48-49 0.3521284314873583 1107 50-51 0.3892795938420832 1107 52-53 0.32238283578845994 1107 54-55 0.4315129035809946 1107 56-57 0.3067267798791775 1107 58-59 0.4586392733128477 1107 60-61 0.31429807679392496 1107 62-63 0.36336796086106204 1107 64-65 0.3739568331885792 1107 66-67 0.500999684654154 1107 68-69 0.8480106241924403 1107 70-71 0.5487151742473664 1107 72-73 0.436865705716837 1107 74-75 0.5200478121270962 1107 76-77 0.5275331074737721 1107 78-79 0.6401105956551874 1107 80-81 0.6428590133996401 1107 82-83 0.675634641814046 1107 84-85 0.6171865755068495 1107 86-87 0.6579869826310549 1107 88-89 0.8192399130390982 1107 90-91 0.8920182143634818 1107 92-93 0.9329479261279268 1107 94-95 0.9081730998483799 1107 96-97 0.8133757590325743 1107 98-99 0.7683922620753059 1107 100-101 1.3011498877724144 1108 1 0.00546237715825626 1108 2 0.05649171251879892 1108 3 0.07190896111600864 1108 4 0.1603935791007416 1108 5 -0.018840706074868763 1108 6 0.0391095043751406 1108 7 0.04538072818144201 1108 8 0.06066102086560221 1108 9 0.22791620618326647 1108 10-11 0.15240527502385248 1108 12-13 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-1.731338423156867 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3451.0 3 770.0 4 728.0 5 830.0 6 940.0 7 1181.0 8 1424.0 9 1823.0 10 2522.0 11 3405.0 12 5012.0 13 7414.0 14 6862.0 15 8087.0 16 10347.0 17 14631.0 18 11920.0 19 7432.0 20 7321.0 21 7519.0 22 8110.0 23 9137.0 24 10134.0 25 11364.0 26 13343.0 27 15371.0 28 17858.0 29 21151.0 30 25095.0 31 29871.0 32 36544.0 33 46550.0 34 62898.0 35 92093.0 36 128234.0 37 176045.0 38 138335.0 39 17661.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.41672922799152 19.05484328778591 14.930064942299543 18.59836254192303 2 14.411474743948945 23.636169087711316 38.98063683706957 22.971719331270172 3 22.387828654484103 28.278804766223768 31.57493379378986 17.75843278550227 4 11.484605769940082 16.71104895628024 40.626782521239605 31.177562752540076 5 11.184563957143347 41.03448670003564 34.225574128896234 13.555375213924775 6 25.72502411987135 38.288750948957265 18.110622194020348 17.87560273715104 7 24.175290066093112 37.29192716879707 21.337565064950713 17.195217700159105 8 23.59692460121699 30.98652254153481 21.196696947761218 24.21985590948698 9 25.38077181525206 19.889868276060476 23.422932200027628 31.30642770865984 10-11 23.553432975948578 25.870609305781798 27.473518096075868 23.102439622193753 12-13 29.840411141361777 24.680113472024825 26.432355832317718 19.047119554295676 14-15 25.219739734271613 25.800588949740916 29.849761216241973 19.12991009974551 16-17 18.666738671785954 29.29703098314509 31.006927550214485 21.029302794854473 18-19 18.214643108821313 30.64726950945736 32.442929452655555 18.695157929065772 20-21 20.98049866743664 27.400639381142206 30.144650116430515 21.47421183499064 22-23 19.698649845351586 26.93209233717769 30.18892816055388 23.180329656916847 24-25 21.624426548319537 27.048115920348394 31.11167555932316 20.21578197200891 26-27 22.312743342239436 24.64779002655209 33.62226220502869 19.417204426179783 28-29 19.35841414661332 27.20530529163159 33.691489687704355 19.744790874050743 30-31 19.47391409801566 28.539435668036234 31.922061963398246 20.06458827054986 32-33 19.403260241290468 28.482215331308296 31.29775551358226 20.816768913818972 34-35 21.288284855148518 25.9584228763723 31.242867792826562 21.510424475652623 36-37 21.910105620967116 25.771088339764393 32.91949954029091 19.399306498977584 38-39 22.597758081334725 25.454431699687174 33.04567257559958 18.90213764337852 40-41 19.917190459857235 26.96632259127242 33.720987208985925 19.39549973988442 42-43 18.55306869596912 29.062050747509215 31.028398102169763 21.356482454351905 44-45 19.875282772689868 28.401068408828035 29.901054328860077 21.82259448962202 46-47 20.964991688028885 28.076628656172236 28.506827914039413 22.451551741759467 48-49 21.265555675510473 27.429308741532093 29.46171012946265 21.843425453494785 50-51 21.45590332918717 28.442476537666206 30.641195138296734 19.46042499484989 52-53 20.267080544508033 29.183550859099 30.312640484303827 20.236728112089132 54-55 18.39691539605966 27.706107413593596 31.52134405963957 22.375633130707172 56-57 21.0207132135694 29.313141308547063 27.98121634930458 21.684929128578958 58-59 21.30670015362088 27.608388210965728 29.011462400937464 22.073449234475927 60-61 20.338312740240447 27.07835831200034 31.13094143252477 21.45238751523444 62-63 22.62291987564879 26.759182685940203 30.009114639007112 20.608782799403897 64-65 21.26174024792733 28.288051755143595 30.56913342262389 19.88107457430518 66-67 19.852291854770524 27.48337513998578 30.205528075657785 22.458804929585913 68-69 19.00938799613546 29.468896915670793 30.25067347760674 21.271041610587005 70-71 20.87235767353203 28.812039441492445 27.97931731705205 22.33628556792348 72-73 19.97803035795599 27.166881129736158 28.986313831016314 23.868774681291534 74-75 19.837655445015717 29.671852644306124 28.703022235528962 21.787469675149193 76-77 21.377277328067173 26.68386824936524 29.377942724778677 22.560911697788914 78-79 20.02939480486709 26.726592861619185 31.199889886789855 22.044122446723872 80-81 20.34269559812963 29.106288505416305 29.206658073988795 21.344357822465266 82-83 19.649630347293122 28.082916090034864 29.53477676397187 22.73267679870014 84-85 19.831862135711724 27.150322884771015 29.094827586206897 23.92298739331036 86-87 20.650977979173295 27.617756409922144 29.363570471338505 22.36769513956605 88-89 21.631755262784623 25.927818708229385 29.407556048760547 23.032869980225446 90-91 20.050580861053362 25.917032366952725 30.724821965591666 23.30756480640225 92-93 20.009951930298932 28.325551215929767 29.721377247008164 21.94311960676314 94-95 19.58396297720137 28.11356175952186 28.85927570394489 23.44319955933188 96-97 18.481663650161952 26.96045857826135 29.051444010369128 25.506433761207575 98-99 20.09499398593972 27.074120554524523 28.164579740806 24.666305718729756 100-101 21.21621248127225 25.75342522902218 29.354002823307567 23.676359466397997 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5828.0 1 3561.5 2 1402.0 3 1431.5 4 1173.0 5 1166.5 6 1872.0 7 3383.5 8 4837.5 9 5134.0 10 4026.5 11 2930.5 12 2736.5 13 2708.0 14 2784.5 15 2971.0 16 3132.5 17 3090.5 18 3051.5 19 3025.0 20 3064.5 21 3447.5 22 3851.0 23 4170.5 24 4465.0 25 5043.0 26 6059.5 27 7152.5 28 7956.0 29 8927.0 30 10429.5 31 12238.5 32 14716.5 33 17372.5 34 19306.0 35 20572.5 36 22886.5 37 25514.0 38 25572.5 39 24579.5 40 23832.5 41 23098.5 42 22005.0 43 22118.0 44 23294.0 45 23952.0 46 28721.5 47 45076.0 48 104044.5 49 105879.5 50 48095.0 51 29204.5 52 20734.0 53 17935.0 54 16525.0 55 14867.5 56 13482.0 57 12243.5 58 11408.5 59 10604.0 60 9600.5 61 8355.0 62 6760.5 63 4825.5 64 3187.5 65 2343.0 66 1676.5 67 1254.0 68 960.5 69 648.0 70 457.0 71 355.5 72 294.0 73 251.0 74 189.0 75 143.0 76 104.5 77 60.0 78 46.5 79 33.0 80 20.0 81 14.5 82 14.0 83 14.0 84 6.5 85 3.5 86 4.0 87 2.5 88 1.5 89 2.5 90 1.5 91 1.0 92 1.5 93 1.5 94 1.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3136764814259305 2 0.08428368726600119 3 0.09237990353046928 4 0.09777738104011467 5 0.10815714548174044 6 0.05335198922995642 7 0.054908953896200284 8 0.056673513851276655 9 0.05874946673960182 10-11 0.05589503151815473 12-13 0.05527224565165718 14-15 0.05319629276333203 16-17 0.05381907862982957 18-19 0.061396306672216384 20-21 0.06300517016066837 22-23 0.07738114391232005 24-25 0.0852178660657475 26-27 0.08065076971143217 28-29 0.07800392977881761 30-31 0.0726064522691722 32-33 0.06554821244886669 34-35 0.30942077800486395 36-37 0.4831261359354711 38-39 0.4580590048089449 40-41 0.43844125001427214 42-43 0.4700476327390226 44-45 0.47788435489245007 46-47 0.4727982703160534 48-49 0.48987298282252784 50-51 0.48738183935653767 52-53 0.4853577852904206 54-55 0.47606789611516553 56-57 0.4554121648763303 58-59 0.4394273276362266 60-61 0.39728548400322605 62-63 0.4687501621838194 64-65 0.49251982275514244 66-67 0.5017059142859812 68-69 0.5143173280825565 70-71 0.51172238697215 72-73 0.5000451519753211 74-75 0.5018097119303975 76-77 0.47606789611516553 78-79 0.45670963543153353 80-81 0.46262610116326025 82-83 0.47124130564980954 84-85 0.473005865604886 86-87 0.4720716868051396 88-89 0.47798815253686633 90-91 0.49303881097722363 92-93 0.4987476814201179 94-95 0.5053388318405503 96-97 0.49802109790920407 98-99 0.5009274319528593 100-101 0.4783514442923232 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 963413.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.75602544839461 #Duplication Level Percentage of deduplicated Percentage of total 1 69.92610257836445 21.506489904072286 2 13.176972713759245 8.105426162343614 3 5.734966269281208 5.291543055710926 4 3.0418469048103827 3.7422048325787403 5 1.8339026669929612 2.8201778547957126 6 1.258445207222202 2.3222863691241766 7 0.8562827259125805 1.8435097316431621 8 0.6229719062527151 1.5328111841874728 9 0.4579344344370346 1.2675818807317465 >10 2.7149410785664356 15.58726595823377 >50 0.22673376132088208 4.8457005194705 >100 0.12453433893954524 6.714682506382495 >500 0.005414536475631513 1.178925640167826 >1k 0.014551566778259691 10.597841039887749 >5k 0.0030456767675427258 6.389117096083885 >10k+ 0.0013536341189078782 6.254436264585945 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 18965 1.968522326354326 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 15812 1.6412483535098654 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 13600 1.4116479640611035 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 11715 1.215989404336458 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9111 0.9457003382765231 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 8260 0.857368542878288 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7506 0.7791051189884297 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 7147 0.7418417646429932 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 6498 0.674477093416842 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 6436 0.6680416394630341 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5876 0.6099149585899297 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 5500 0.5708870442894168 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5052 0.5243856995909335 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 4767 0.4948033709323001 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4567 0.4740438420490486 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4341 0.4505855744109743 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 4280 0.4442539181015826 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 4276 0.44383872752391756 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4085 0.42401337744041234 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3727 0.3868538207393921 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3651 0.3789651997637566 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3518 0.3651601130563943 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3381 0.350939835771367 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 3236 0.33588917733100965 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 3223 0.33453980795359833 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3150 0.32696257991121147 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3084 0.3201119353797385 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3021 0.31357268378151426 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 2809 0.29156758316526765 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 2660 0.27610173414724526 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2607 0.27060045899318363 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2308 0.23956496331272256 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2074 0.21527631451931828 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2003 0.207906681765764 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1964 0.20385857363352994 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1922 0.19949907256804714 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1878 0.1949319762137318 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1850 0.1920256421700766 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1806 0.18745854581576127 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1782 0.18496740234977108 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1696 0.17604080492997293 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1607 0.166802814576926 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1463 0.1518559537809849 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1438 0.14926101267057845 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1400 0.14531670218276066 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1314 0.1363901047629625 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1229 0.12756730498758062 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1207 0.12528375681042295 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 1105 0.11469639707996467 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1102 0.1143850041467159 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1084 0.11251664654722325 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1079 0.11199765832514198 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1061 0.11012930072564933 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1060 0.11002550308123309 No Hit GCTTTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1005 0.1043166326383389 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1003 0.10410903734950638 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 969 0.10057991743935363 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.189882220812881E-5 0.0 12-13 0.0 0.0 0.0 1.0379764441625762E-4 0.0 14-15 0.0 0.0 0.0 1.0379764441625762E-4 0.0 16-17 0.0 0.0 0.0 1.556964666243864E-4 0.0 18-19 0.0 0.0 0.0 2.5949411104064405E-4 0.0 20-21 0.0 0.0 0.0 7.265835109138033E-4 0.0 22-23 0.0 0.0 0.0 0.0017645599550763795 0.0 24-25 0.0 0.0 0.0 0.003010131688071471 0.0 26-27 0.0 0.0 0.0 0.006124061020559199 0.0 28-29 0.0 0.0 0.0 0.018579778350510112 0.0 30-31 0.0 0.0 0.0 0.04421779652132574 0.0 32-33 0.0 0.0 0.0 0.09523433875191636 0.0 34-35 0.0 0.0 0.0 0.17624840021880545 0.0 36-37 0.0 0.0 0.0 0.28964732674356686 0.0 38-39 0.0 0.0 0.0 0.4418665722800087 0.0 40-41 0.0 0.0 0.0 0.6181149724988142 0.0 42-43 0.0 0.0 0.0 0.7943114738954113 0.0 44-45 0.0 0.0 0.0 0.9858700266656149 0.0 46-47 0.0 0.0 0.0 1.2266805617113326 0.0 48-49 0.0 0.0 0.0 1.491520251439414 0.0 50-51 0.0 0.0 0.0 1.777275166517371 0.0 52-53 0.0 0.0 0.0 2.0198502615181653 0.0 54-55 0.0 0.0 0.0 2.2790848784477684 0.0 56-57 0.0 0.0 0.0 2.5869486917863886 0.0 58-59 0.0 0.0 0.0 2.951330322509661 0.0 60-61 0.0 0.0 0.0 3.4504931945074437 0.0 62-63 0.0 0.0 0.0 3.903777507673241 0.0 64-65 0.0 0.0 0.0 4.283417392125703 0.0 66-67 0.0 0.0 0.0 4.714021919986548 0.0 68-69 0.0 0.0 0.0 5.211679726140295 0.0 70-71 0.0 0.0 0.0 5.7761313164759045 0.0 72-73 0.0 0.0 0.0 6.378469047023447 0.0 74-75 0.0 0.0 0.0 7.003071372298278 0.0 76-77 0.0 0.0 0.0 7.554392560615229 0.0 78-79 0.0 0.0 0.0 8.249006397048825 0.0 80-81 0.0 0.0 0.0 9.003303879021768 0.0 82-83 0.0 0.0 0.0 9.744626655442682 0.0 84-85 0.0 0.0 0.0 10.633342086934679 0.0 86-87 0.0 0.0 0.0 11.57603229352313 0.0 88-89 0.0 0.0 0.0 12.399147613744054 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGCGGT 55 0.0026183268 34.440144 6 ATATGAG 365 0.0 28.554726 3 GGATATG 355 0.0 28.078571 1 TTAGCGC 90 9.085307E-4 26.31938 4 ATTGGCG 110 9.673766E-5 25.830107 7 GTATAGG 295 0.0 25.744356 1 TAAGGTA 130 1.0225953E-5 25.509554 4 CATATAG 190 1.2243618E-8 24.934149 3 TTTGCGT 725 0.0 24.830975 3 CTTTGCG 710 0.0 24.68832 2 GATATGA 405 0.0 24.564754 2 CATGGGG 1530 0.0 24.46154 4 GAGTACT 6030 0.0 24.032244 12-13 CGCGCGG 50 0.001639395 23.786335 30-31 TATATGG 160 1.90217E-6 23.687443 2 CTTAGCG 100 0.0016790276 23.687443 3 GATTGGC 395 0.0 22.778448 9 AGTACTT 6310 0.0 22.74068 12-13 CATGGGT 450 0.0 22.108278 4 GTACATG 8070 0.0 21.645037 1 >>END_MODULE