##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938967_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 963413 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.067687481900286 33.0 31.0 34.0 30.0 34.0 2 32.28498058465061 34.0 31.0 34.0 30.0 34.0 3 32.35014266986225 34.0 31.0 34.0 30.0 34.0 4 35.89751020590339 37.0 35.0 37.0 35.0 37.0 5 35.84246839102234 37.0 35.0 37.0 35.0 37.0 6 35.92999160277056 37.0 35.0 37.0 35.0 37.0 7 35.87537328227873 37.0 35.0 37.0 35.0 37.0 8 35.87768485581988 37.0 35.0 37.0 35.0 37.0 9 37.47781065856491 39.0 37.0 39.0 34.0 39.0 10-11 37.39373197164664 39.0 37.0 39.0 34.0 39.0 12-13 37.309962082720496 39.0 37.0 39.0 34.0 39.0 14-15 38.38099029180631 40.0 38.0 41.0 33.5 41.0 16-17 38.51545391228892 40.0 38.0 41.0 34.0 41.0 18-19 38.69594140830568 40.0 38.0 41.0 34.0 41.0 20-21 38.68400312223314 40.0 38.0 41.0 34.0 41.0 22-23 38.63002834713669 40.0 38.0 41.0 34.0 41.0 24-25 38.505372047086766 40.0 38.0 41.0 34.0 41.0 26-27 38.197714791060534 40.0 37.5 41.0 33.5 41.0 28-29 38.009926168735525 40.0 37.0 41.0 33.0 41.0 30-31 37.820675037600694 40.0 37.0 41.0 33.0 41.0 32-33 37.65016664711811 40.0 36.0 41.0 33.0 41.0 34-35 37.500427646294995 40.0 36.0 41.0 33.0 41.0 36-37 37.27546026470475 40.0 35.5 41.0 32.5 41.0 38-39 37.021191327084026 39.5 35.0 41.0 31.5 41.0 40-41 36.80755034445248 39.0 35.0 41.0 31.0 41.0 42-43 36.612307494293724 39.0 35.0 41.0 31.0 41.0 44-45 36.3110363883402 39.0 35.0 41.0 30.0 41.0 46-47 35.813522860912194 39.0 35.0 41.0 28.5 41.0 48-49 35.33619641835848 39.0 35.0 40.5 26.5 41.0 50-51 35.037833203413285 39.0 35.0 40.0 25.5 41.0 52-53 34.671814164849344 38.0 34.0 40.0 24.0 41.0 54-55 34.42174539890992 38.0 34.0 40.0 23.0 41.0 56-57 34.361962626620155 38.0 34.0 40.0 23.0 41.0 58-59 34.14557515831736 38.0 34.0 40.0 23.0 41.0 60-61 33.713706375147524 37.5 33.5 40.0 22.0 41.0 62-63 33.23564815920068 37.0 33.0 39.5 20.5 41.0 64-65 32.708043175668166 36.0 32.0 39.0 18.5 40.5 66-67 32.223242264740044 36.0 32.0 39.0 15.5 40.0 68-69 31.748498826567626 35.0 31.0 38.0 12.0 40.0 70-71 31.152591879079896 35.0 31.0 37.0 7.5 39.0 72-73 30.581177023768625 34.5 30.0 37.0 6.0 39.0 74-75 30.055916310035258 34.0 29.5 36.0 2.0 38.5 76-77 28.117518136043422 32.0 27.0 34.5 2.0 36.0 78-79 29.035399667639943 34.0 29.0 35.0 2.0 37.0 80-81 29.060336013734506 34.0 29.0 35.0 2.0 36.5 82-83 28.66094862743185 34.0 29.0 35.0 2.0 36.0 84-85 28.23695600952032 34.0 28.5 35.0 2.0 36.0 86-87 27.576155293731766 33.0 26.5 35.0 2.0 35.0 88-89 26.805365404037524 32.5 25.5 35.0 2.0 35.0 90-91 26.13922170450264 32.0 24.5 34.0 2.0 35.0 92-93 25.814053266875163 32.0 24.0 34.0 2.0 35.0 94-95 25.305796683250072 32.0 21.5 34.0 2.0 35.0 96-97 24.38288823173447 31.0 12.5 34.0 2.0 35.0 98-99 23.22947738924013 31.0 2.0 34.0 2.0 35.0 100-101 17.97473253941975 23.5 2.0 30.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.26311509004004563 1101 2 0.2555049122371642 1101 3 0.23879881831809513 1101 4 0.1328996195265688 1101 5 0.1786966748450709 1101 6 0.14544766579020774 1101 7 0.31359868364135224 1101 8 0.18664853184949948 1101 9 0.41583268806598284 1101 10-11 0.46734483532749493 1101 12-13 0.36415122362394925 1101 14-15 0.44898520825873334 1101 16-17 0.4011681913489866 1101 18-19 0.3637602483426079 1101 20-21 0.45924842278018474 1101 22-23 0.37012843237899773 1101 24-25 0.3703160245098829 1101 26-27 0.5862028109635204 1101 28-29 0.6057573838183714 1101 30-31 0.46605957459119196 1101 32-33 0.47524878346004584 1101 34-35 0.5445545939527321 1101 36-37 0.47844485317259 1101 38-39 0.5613512372023735 1101 40-41 0.5326111795756603 1101 42-43 0.6785223440790276 1101 44-45 0.5869328487328858 1101 46-47 0.581052957166321 1101 48-49 0.6853666748631326 1101 50-51 0.6796720162357417 1101 52-53 0.6366458505690531 1101 54-55 0.4223008228434395 1101 56-57 0.5392122267228956 1101 58-59 0.4820975456294079 1101 60-61 0.4971144377436758 1101 62-63 0.5223219266765753 1101 64-65 0.6037166353087429 1101 66-67 0.33172458907457525 1101 68-69 0.1895495774454723 1101 70-71 0.3280330970346128 1101 72-73 0.551314563683782 1101 74-75 0.5906146428765133 1101 76-77 0.33002034741240394 1101 78-79 0.8280770974614526 1101 80-81 0.8248206415257933 1101 82-83 0.6659557003377898 1101 84-85 0.7615910498181755 1101 86-87 0.916416173999508 1101 88-89 0.8766015998422851 1101 90-91 0.8089437120521943 1101 92-93 0.8134639350461015 1101 94-95 0.4397029784746387 1101 96-97 0.5852167482506268 1101 98-99 1.2150778185496556 1101 100-101 1.1960858763117237 1102 1 0.10749163674530138 1102 2 0.04307149920918363 1102 3 0.12017832211407864 1102 4 0.08400276117330918 1102 5 0.15425479950427246 1102 6 0.13360095214564893 1102 7 0.11691675964488013 1102 8 0.07161822691262643 1102 9 0.1881458775295144 1102 10-11 0.17410852497977913 1102 12-13 0.1897398891581119 1102 14-15 0.33825815192720654 1102 16-17 0.3374976762699191 1102 18-19 0.16124606584187262 1102 20-21 0.07022452487321829 1102 22-23 0.27151876311176437 1102 24-25 0.26037346232708813 1102 26-27 0.29363761340469097 1102 28-29 0.2656119935243382 1102 30-31 0.2176003813945968 1102 32-33 0.33340608076300526 1102 34-35 0.36012309988787905 1102 36-37 0.3493913057123308 1102 38-39 0.361936363665194 1102 40-41 0.4573718334911874 1102 42-43 0.3685403278045456 1102 44-45 0.3028070255711057 1102 46-47 0.7493489074200852 1102 48-49 0.7966213149788999 1102 50-51 0.6331358495481112 1102 52-53 0.6059162251338748 1102 54-55 0.4867092998368463 1102 56-57 0.6079088784992521 1102 58-59 0.5548851017302425 1102 60-61 0.4856799603727495 1102 62-63 0.590008632351676 1102 64-65 0.6108144395115929 1102 66-67 0.7847755307297035 1102 68-69 0.7778788998312827 1102 70-71 0.7889610939726595 1102 72-73 1.0849619568300426 1102 74-75 1.0524719737351198 1102 76-77 0.6604287038152385 1102 78-79 0.7357073352870458 1102 80-81 0.9523582867587947 1102 82-83 1.02381676426128 1102 84-85 1.1559995792422804 1102 86-87 1.210783676411321 1102 88-89 1.1673186156973223 1102 90-91 1.1888839935001165 1102 92-93 1.175081002283214 1102 94-95 0.7746636292443227 1102 96-97 0.7098058227441477 1102 98-99 0.7176593744827144 1102 100-101 1.047429805886825 1103 1 -0.05722050270315293 1103 2 0.015256768857398129 1103 3 0.05594287087036065 1103 4 -0.0013788005480463994 1103 5 0.07297101429374919 1103 6 0.06398494217901174 1103 7 0.1212204147823428 1103 8 0.10057056865003489 1103 9 0.11532687123702345 1103 10-11 -0.02666206816182637 1103 12-13 -0.00961990749715369 1103 14-15 0.13844634610769901 1103 16-17 0.08495454733711227 1103 18-19 0.13055893272814245 1103 20-21 0.22425473096300408 1103 22-23 0.10626883173797097 1103 24-25 0.1255856492860019 1103 26-27 0.038092747700900986 1103 28-29 0.03771359198711366 1103 30-31 0.19164845669337893 1103 32-33 0.10059760595410694 1103 34-35 0.10483228313041337 1103 36-37 0.2728988176427336 1103 38-39 0.12371895583546433 1103 40-41 0.13376098632702593 1103 42-43 0.15509038270550946 1103 44-45 0.2779603787532494 1103 46-47 0.2309927162802552 1103 48-49 0.0265263732583918 1103 50-51 -0.061655685145083794 1103 52-53 0.26592614953946736 1103 54-55 0.4212742096725677 1103 56-57 0.383545386586583 1103 58-59 0.33928596853205306 1103 60-61 0.19187070428085917 1103 62-63 0.46197192260886055 1103 64-65 0.5563239099284161 1103 66-67 0.4506477170551406 1103 68-69 0.4240151122980258 1103 70-71 0.4778831082046793 1103 72-73 0.3287512849266676 1103 74-75 0.39273721403242945 1103 76-77 0.11204260748515438 1103 78-79 0.46425636607381904 1103 80-81 0.17080606749278004 1103 82-83 0.1022307861794367 1103 84-85 0.43785665724388423 1103 86-87 0.39316112583908236 1103 88-89 0.5501043910679044 1103 90-91 0.8388969269010218 1103 92-93 0.5665169793092701 1103 94-95 0.8200328118533875 1103 96-97 0.742443523871728 1103 98-99 0.6901052917511805 1103 100-101 0.713901811672546 1104 1 -0.0032876668246331064 1104 2 -0.005952411835039584 1104 3 -0.07250780362791431 1104 4 -0.08619621884508177 1104 5 -0.149902984125859 1104 6 -0.08737241964600884 1104 7 -0.05168588645494765 1104 8 -0.166912205325481 1104 9 0.04819363691418488 1104 10-11 0.0021891097970936357 1104 12-13 0.07171176650950883 1104 14-15 0.06514919364612126 1104 16-17 -0.03375315978057358 1104 18-19 0.16145638479513735 1104 20-21 0.07780907047977337 1104 22-23 0.09806571779783013 1104 24-25 0.12462106913253734 1104 26-27 0.0451150713931483 1104 28-29 0.18091224015923757 1104 30-31 0.07450119445370262 1104 32-33 0.09154059083741117 1104 34-35 0.07313047803352646 1104 36-37 0.07735223080220521 1104 38-39 0.2991071687818163 1104 40-41 0.022335569800858934 1104 42-43 -0.07596420776940249 1104 44-45 -0.027926713246721135 1104 46-47 0.1388987987335213 1104 48-49 0.024984589370674826 1104 50-51 -0.16702979295669707 1104 52-53 -0.04274400394395883 1104 54-55 0.16095789945227068 1104 56-57 -0.09762760465433473 1104 58-59 -0.1706983047575008 1104 60-61 -0.15147543006283115 1104 62-63 -0.06885925076846888 1104 64-65 -0.01645073480494119 1104 66-67 -8.349951460573379E-4 1104 68-69 -0.12400926796405898 1104 70-71 -0.1888509096223565 1104 72-73 -0.034922459188685195 1104 74-75 0.05508332521074877 1104 76-77 0.07559962388880948 1104 78-79 0.08178521339088007 1104 80-81 0.07232108251166025 1104 82-83 -0.14392762374975376 1104 84-85 0.032423477745748386 1104 86-87 0.27132287672901967 1104 88-89 0.4278646089253151 1104 90-91 0.7266813139582737 1104 92-93 0.6244529193470072 1104 94-95 0.7565487663566017 1104 96-97 0.5011942413546286 1104 98-99 0.3050331831107549 1104 100-101 0.8275054162036923 1105 1 0.06091073207013409 1105 2 0.05592820837736667 1105 3 0.03837428446987445 1105 4 0.03643141933967087 1105 5 0.11477771996571562 1105 6 0.015423963681776343 1105 7 -0.06875036239519261 1105 8 -0.005393005631390224 1105 9 0.028837286151251362 1105 10-11 0.03889487895670385 1105 12-13 0.2145178946225954 1105 14-15 3.4452108324956043E-4 1105 16-17 -0.041232590882472664 1105 18-19 0.0773416183616078 1105 20-21 0.07907774284529268 1105 22-23 0.0034537206102456253 1105 24-25 0.08162991345034243 1105 26-27 2.5760429722510025E-4 1105 28-29 0.1687047379080724 1105 30-31 0.07445185376860763 1105 32-33 0.0031560048674350583 1105 34-35 0.3795941077810241 1105 36-37 0.12187726503231033 1105 38-39 0.20264674617884992 1105 40-41 0.09296305996355869 1105 42-43 0.1633300970820173 1105 44-45 0.2803760905377146 1105 46-47 0.4440931393569443 1105 48-49 0.2153656228113192 1105 50-51 0.37559562489722964 1105 52-53 0.5650388723712823 1105 54-55 0.5690787594604103 1105 56-57 0.34879951298619005 1105 58-59 0.40557005922872946 1105 60-61 0.038710090913127715 1105 62-63 0.0339180130438379 1105 64-65 -0.2294291852607202 1105 66-67 -0.05251196168074301 1105 68-69 -0.06291686519604411 1105 70-71 0.032879961610788655 1105 72-73 0.08210069392779928 1105 74-75 0.23364418077823146 1105 76-77 -0.09359945344037968 1105 78-79 0.1762127997679208 1105 80-81 0.15000552416013235 1105 82-83 0.18205333074579855 1105 84-85 0.1616424147006903 1105 86-87 0.30323396481447773 1105 88-89 0.15999279602518968 1105 90-91 0.34143787935318315 1105 92-93 0.50313959366148 1105 94-95 0.31755790671851614 1105 96-97 0.33450413786254174 1105 98-99 0.645283463735904 1105 100-101 0.5622085703069857 1106 1 0.013615911979002249 1106 2 -0.0190661176408895 1106 3 0.008841645981078727 1106 4 -0.019624028555718098 1106 5 0.07901592244830624 1106 6 -0.02302991994049819 1106 7 0.01116374225048844 1106 8 0.051657571368906474 1106 9 -0.04415281493291445 1106 10-11 0.04822290493481063 1106 12-13 -0.001371000085761409 1106 14-15 -0.014950787843233115 1106 16-17 -0.08607275918150492 1106 18-19 0.02296916074188715 1106 20-21 -0.0631139853622571 1106 22-23 0.1391509220278735 1106 24-25 0.17848041712533558 1106 26-27 0.1039133943773507 1106 28-29 0.07838193199316379 1106 30-31 0.08779880465811374 1106 32-33 0.246613467462808 1106 34-35 0.28002533039315125 1106 36-37 0.15341412845520352 1106 38-39 0.23760196536913014 1106 40-41 0.2950056933894203 1106 42-43 0.1852299207521213 1106 44-45 0.1980893886050623 1106 46-47 0.35282408787210073 1106 48-49 0.4176998116065178 1106 50-51 0.2178484575234947 1106 52-53 0.2697282000480854 1106 54-55 0.3039868409562416 1106 56-57 0.1215888424088476 1106 58-59 -0.013684816030966829 1106 60-61 -0.05309318777538863 1106 62-63 0.15631365542586906 1106 64-65 0.2633814434164563 1106 66-67 0.09236486926952381 1106 68-69 0.2619543790645018 1106 70-71 0.2713355910735942 1106 72-73 0.1518697192250258 1106 74-75 0.0304719885262692 1106 76-77 0.04520010660780471 1106 78-79 0.18359072186851577 1106 80-81 0.32570167807343253 1106 82-83 0.17423720267093756 1106 84-85 0.15638045132842038 1106 86-87 0.3370945419893552 1106 88-89 0.5647327530775144 1106 90-91 0.6578854430327183 1106 92-93 0.8628176606668418 1106 94-95 0.9573176790609104 1106 96-97 0.7897390407065181 1106 98-99 1.063045645338235 1106 100-101 0.9373041550762728 1107 1 -0.034555601170580985 1107 2 -0.010993158669030834 1107 3 -0.013276637082405784 1107 4 -0.02008543719455247 1107 5 0.02739117740760122 1107 6 0.027158704813572854 1107 7 -0.07946977519505083 1107 8 0.03815149747132551 1107 9 -0.08360945831480393 1107 10-11 0.12525220043489327 1107 12-13 -0.04975516733905749 1107 14-15 -0.025520881343354063 1107 16-17 -0.07791349761582467 1107 18-19 -0.014846605219020148 1107 20-21 0.013001517968007192 1107 22-23 0.060311528334615616 1107 24-25 -0.08319017546323693 1107 26-27 0.08262654937455949 1107 28-29 0.08044642541385372 1107 30-31 0.006608934439164216 1107 32-33 0.04414250574731682 1107 34-35 0.10313942129403131 1107 36-37 -0.0010579252983404785 1107 38-39 -0.10081081147605175 1107 40-41 0.1332977594171112 1107 42-43 0.16334572642350054 1107 44-45 0.17090181527606063 1107 46-47 0.31210392939120624 1107 48-49 0.10008765207871306 1107 50-51 0.13435210817961973 1107 52-53 0.2710723770485046 1107 54-55 0.025217617476528176 1107 56-57 0.04692920178863602 1107 58-59 -0.0768729805045325 1107 60-61 0.10227404386302652 1107 62-63 0.11237209751174504 1107 64-65 0.08074146399369653 1107 66-67 0.050469190491156724 1107 68-69 0.0966465551985074 1107 70-71 -0.006893818142845731 1107 72-73 -0.056477151770081946 1107 74-75 -0.044740439949752187 1107 76-77 -0.13481807898451237 1107 78-79 0.0879319115582291 1107 80-81 0.02270511663952135 1107 82-83 -0.268879798827232 1107 84-85 -0.21284065930134943 1107 86-87 -0.1048452470036203 1107 88-89 0.18929954790071335 1107 90-91 -0.03920373405794919 1107 92-93 0.29288153586764665 1107 94-95 0.6221702990497207 1107 96-97 0.7929920280172169 1107 98-99 0.8443954855938252 1107 100-101 0.7660719119759669 1108 1 -0.056802536996542585 1108 2 0.0329047710191972 1108 3 0.07698510813597892 1108 4 -0.02633930847108701 1108 5 -6.263066849712118E-4 1108 6 0.1201350360634521 1108 7 0.013176910992235946 1108 8 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2316 66-67 -1.3533151461540776 2316 68-69 -1.1598006978698727 2316 70-71 -0.9674285931282327 2316 72-73 -1.0055712487893693 2316 74-75 -1.2442034732636031 2316 76-77 -1.0780091952907647 2316 78-79 -1.0569650785060567 2316 80-81 -1.220566362027924 2316 82-83 -1.1784940594935058 2316 84-85 -1.4443755804001235 2316 86-87 -1.2459652060918742 2316 88-89 -1.5653322463716428 2316 90-91 -1.7141891227195494 2316 92-93 -1.7014675290066954 2316 94-95 -1.7379042187761158 2316 96-97 -1.7142004033094942 2316 98-99 -1.762070031647557 2316 100-101 -1.3189295491578719 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 2.0 4 15.0 5 53.0 6 137.0 7 214.0 8 434.0 9 824.0 10 1452.0 11 2193.0 12 3201.0 13 4489.0 14 5337.0 15 7413.0 16 9866.0 17 14253.0 18 13401.0 19 8102.0 20 7097.0 21 7459.0 22 8156.0 23 8588.0 24 9902.0 25 11248.0 26 13237.0 27 15733.0 28 18943.0 29 22935.0 30 27382.0 31 32831.0 32 40600.0 33 51464.0 34 69812.0 35 100436.0 36 145926.0 37 191193.0 38 104893.0 39 4190.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.19467311541375 19.78582435638427 15.544508962014977 18.474993566187003 2 14.665880572506287 24.235608197107574 38.36319418567115 22.735317044714986 3 22.35946577428372 28.300220154803807 31.65786635638091 17.682447714531566 4 11.724047734460715 17.327459770627964 39.92586772235791 31.022624772553414 5 11.029018707449453 41.43467028159263 34.13935664144038 13.39695436951754 6 25.834714706984443 38.70053653002399 17.47817395032037 17.9865748126712 7 23.949541889096366 37.340994983459844 21.110883909600556 17.59857921784323 8 23.869306309962603 31.452658413369967 20.861769563001538 23.816265713665892 9 25.07751089096784 19.801476625289467 23.16628486433129 31.954727619411404 10-11 23.6177527187198 26.21378370439261 27.18491446555112 22.98354911133647 12-13 29.712231410620365 24.80275852619801 26.42911191773414 19.055898145447486 14-15 25.008368750230304 26.013708661617564 29.66533821947249 19.312584368679648 16-17 18.546263806014885 29.498434440340315 30.984691094552748 20.970610659092053 18-19 18.191174158029373 30.612164401534343 32.42837028283404 18.768291157602246 20-21 20.901108870235298 27.3169450692486 30.260231074316003 21.521714986200102 22-23 19.694357456251886 26.927859599154257 30.279537436177424 23.098245508416433 24-25 21.58596572809377 27.241017092358106 31.156212340917133 20.016804838630993 26-27 22.200292086571388 24.79071799944572 33.74570407499172 19.26328583899117 28-29 19.28762638660678 27.265201943507094 33.72811037426317 19.719061295622957 30-31 19.476486200622162 28.548140828492034 31.94154531857054 20.03382765231526 32-33 19.372792353850322 28.511655956479725 31.396036798340898 20.719514891329055 34-35 21.223873918999093 26.08702196804582 31.364585058116205 21.32451905483888 36-37 21.90226427650762 25.853834018809184 32.94198138599532 19.301920318687873 38-39 22.49150070330058 25.464982897598397 33.19744839434661 18.846068004754414 40-41 19.841594847161346 27.031953516495776 33.68367238368094 19.44277925266193 42-43 18.569506740421872 29.099502058924585 31.147786876652404 21.183204324001135 44-45 19.94131183717616 28.371273732428065 29.917780520387755 21.769633910008025 46-47 20.922481436867184 28.15736741119876 28.55092087774412 22.369230274189942 48-49 21.316870505744085 27.465466727909217 29.539668823131 21.6779939432157 50-51 21.3780067801114 28.58976381712383 30.612483210609025 19.41974619215575 52-53 20.352901611250836 29.26849648074087 30.180722078693144 20.197879829315156 54-55 18.372753948721886 27.835362404285597 31.296858149101165 22.49502549789135 56-57 21.00874702749496 29.364457403003698 27.890115661715175 21.736679907786172 58-59 21.29531261271875 27.70918134594555 28.901723004200207 22.09378303713549 60-61 20.22799460244966 27.41483288353747 30.8757525430766 21.481419970936265 62-63 22.474314979624292 27.07986904739662 29.86297516509204 20.582840807887052 64-65 21.13249066464606 28.59200286483134 30.289519232515836 19.985987238006764 66-67 19.771307214067846 27.7324776037912 29.984891868649044 22.51132331349191 68-69 18.989500563118586 29.63316846328311 29.898431053005808 21.478899920592497 70-71 20.84631453297523 29.118056907088086 27.67526890091212 22.36035965902456 72-73 20.007307354166905 27.350056517817382 28.742501917661478 23.900134210354228 74-75 19.75725882876814 29.97572173097104 28.232232697711158 22.034786742549663 76-77 21.18058026496568 27.005306066264794 29.192870184478654 22.621243484290876 78-79 19.866605493349997 27.022482231884933 30.825019748116794 22.28589252664828 80-81 20.151928848679926 29.477538933259567 28.830463410494893 21.540068807565614 82-83 19.52734704638613 28.54751804262554 29.140098794597957 22.785036116390376 84-85 19.571740854380057 27.658208533835992 28.594879449290644 24.175171162493307 86-87 20.308524553035742 28.069324262972582 28.917956384558085 22.704194799433587 88-89 21.178139112087766 26.409592988103626 29.030662452412425 23.381605447396183 90-91 19.791641160586973 26.379008448655544 30.297575850088332 23.53177454066915 92-93 19.493664710773054 28.859689458207438 29.286090181469422 22.36055564955009 94-95 19.11061241669517 28.598475510546194 28.379564347062942 23.9113477256957 96-97 17.880441721255576 27.74822428179815 28.684323337966166 25.687010658980103 98-99 19.48800773915237 27.640741821005115 27.771215460036352 25.100034979806168 100-101 20.369085387974053 26.70923081953294 29.043910241605715 23.877773550887294 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5081.0 1 3237.0 2 1396.0 3 1325.0 4 1086.5 5 1120.5 6 1847.0 7 3243.0 8 4545.5 9 4840.5 10 3848.5 11 2790.0 12 2573.0 13 2584.0 14 2611.0 15 2654.5 16 2682.0 17 2741.5 18 2804.5 19 2916.0 20 3122.5 21 3352.5 22 3657.0 23 4103.0 24 4575.0 25 5090.0 26 6098.5 27 7204.5 28 8011.0 29 8984.0 30 10569.5 31 12476.0 32 14874.5 33 17383.0 34 19290.5 35 20746.0 36 23039.5 37 25607.0 38 25957.5 39 24960.0 40 24004.5 41 23302.0 42 22278.5 43 22483.5 44 23598.0 45 24266.0 46 28399.0 47 45745.5 48 105863.5 49 107028.5 50 50247.0 51 30990.5 52 20278.5 53 16609.5 54 15272.0 55 14129.0 56 13113.5 57 11898.0 58 11208.5 59 10644.5 60 9555.5 61 8087.0 62 6537.5 63 4684.5 64 2979.5 65 2161.5 66 1541.0 67 1130.0 68 838.5 69 543.0 70 384.5 71 295.0 72 229.5 73 198.0 74 152.5 75 107.0 76 74.5 77 32.5 78 12.5 79 9.0 80 5.0 81 2.0 82 2.0 83 1.0 84 0.5 85 0.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3778234256751777 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 7.784823331219322E-4 16-17 0.0020240540661170237 18-19 3.632917554569017E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.013234199663072845 36-37 0.022160797082871 38-39 0.010172169152793247 40-41 0.0062797574871835855 42-43 0.00477469164314785 44-45 0.001660762310660122 46-47 0.0011936729107869626 48-49 0.003165828154695857 50-51 7.265835109138034E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 8.822799775381896E-4 60-61 0.0013493693774113491 62-63 0.0011417740885788339 64-65 5.708870442894169E-4 66-67 0.0025430422881983117 68-69 0.0029063340436552136 70-71 5.1898822208128815E-5 72-73 0.0 74-75 0.0 76-77 0.08672293190978324 78-79 0.003113929332487729 80-81 1.556964666243864E-4 82-83 0.0 84-85 1.0379764441625763E-4 86-87 0.08968116477564658 88-89 0.4285804737947277 90-91 0.3814044444075386 92-93 0.0 94-95 8.822799775381896E-4 96-97 0.0 98-99 0.0 100-101 4.670893998731593E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 963413.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.242983439611503 #Duplication Level Percentage of deduplicated Percentage of total 1 68.2265686119552 19.95148416060924 2 13.86678379320611 8.110122576507989 3 6.003928874222292 5.267183779244634 4 3.2293699279889143 3.777456452982368 5 1.9434122414639023 2.841558599673358 6 1.3115787300080635 2.301268504882349 7 0.9059584735885309 1.8545050048087584 8 0.6721733393401168 1.572508306469712 9 0.5125195200167956 1.3488839852695909 >10 2.931366585283529 16.055174243867697 >50 0.22977771344944606 4.725483294580938 >100 0.14099187467472432 7.283938163020977 >500 0.006037435439471475 1.3440847454347882 >1k 0.014916016968105995 10.881769088387552 >5k 0.0031962893503084274 6.414883832175552 >10k+ 0.0014205730445815234 6.269695262084545 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 19214 1.994367939813974 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 16424 1.704772511892615 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 14197 1.4736151577776093 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 10535 1.093508183925274 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8946 0.9285737269478407 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7965 0.8267482377754919 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7750 0.8044317442259965 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 7462 0.7745380226341143 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 6506 0.6753074745721721 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 6375 0.6617099831536424 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 5802 0.6022339329031268 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5591 0.5803326299312963 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5371 0.5574971481597196 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 4759 0.49397298977697 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 4526 0.46978813862798197 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 4398 0.456502040142701 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4388 0.4554640636985384 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4375 0.45411469432112705 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3970 0.4120766483325427 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3955 0.41051968366629893 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3855 0.40013991922467307 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3746 0.38882597598330104 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 3563 0.3698310070551259 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3559 0.36941581647746086 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3483 0.36152719550182527 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 3391 0.3519778122155296 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3182 0.3302841045325317 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3059 0.31751699426933205 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 2937 0.3048536816505486 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2891 0.3000789900074008 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 2841 0.2948891077865879 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2493 0.25876752752973026 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2096 0.21755986269647595 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 2075 0.21538011216373454 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2050 0.21278517105332811 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 2002 0.20780288412134773 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1972 0.20468895478886 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1904 0.19763071496855447 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1894 0.19659273852439194 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1860 0.19306361861423918 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1772 0.18392942590560848 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1658 0.17209649444215513 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1572 0.16316989702235696 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1488 0.15445089489139133 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1447 0.15019519147032476 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1386 0.14386353516093306 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1241 0.1288128767205757 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1223 0.12694451912108307 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1182 0.1226888157000165 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1179 0.12237742276676775 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1172 0.12165083925585393 No Hit GCTTTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1103 0.11448880179113215 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1074 0.11147867010306069 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1055 0.10950651485915179 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 1003 0.10410903734950638 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 1000 0.10379764441625762 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 988 0.10255207268326251 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.189882220812881E-5 0.0 12-13 0.0 0.0 0.0 1.0379764441625762E-4 0.0 14-15 0.0 0.0 0.0 1.0379764441625762E-4 0.0 16-17 0.0 0.0 0.0 1.556964666243864E-4 0.0 18-19 0.0 0.0 0.0 2.5949411104064405E-4 0.0 20-21 0.0 0.0 0.0 7.265835109138033E-4 0.0 22-23 0.0 0.0 0.0 0.0017645599550763795 0.0 24-25 0.0 0.0 0.0 0.003010131688071471 0.0 26-27 0.0 0.0 0.0 0.006124061020559199 0.0 28-29 0.0 0.0 0.0 0.018839272461550757 0.0 30-31 0.0 0.0 0.0 0.04463298709899077 0.0 32-33 0.0 0.0 0.0 0.09611661872945455 0.0 34-35 0.0 0.0 0.0 0.17822055546271431 0.0 36-37 0.0 0.0 0.0 0.29276125607605463 0.0 38-39 0.0 0.0 0.0 0.4483539250560248 0.0 40-41 0.0 0.0 0.0 0.6268339746297797 0.0 42-43 0.0 0.0 0.0 0.804639339514829 0.0 44-45 0.0 0.0 0.0 0.9979624522401089 0.0 46-47 0.0 0.0 0.0 1.2412641307518166 0.0 48-49 0.0 0.0 0.0 1.5082835710126394 0.0 50-51 0.0 0.0 0.0 1.7959068436900893 0.0 52-53 0.0 0.0 0.0 2.0407135880458327 0.0 54-55 0.0 0.0 0.0 2.3032178307745483 0.0 56-57 0.0 0.0 0.0 2.6160120322229408 0.0 58-59 0.0 0.0 0.0 2.9838189852119497 0.0 60-61 0.0 0.0 0.0 3.4875489535640476 0.0 62-63 0.0 0.0 0.0 3.9438952972401244 0.0 64-65 0.0 0.0 0.0 4.327064301602739 0.0 66-67 0.0 5.189882220812881E-5 0.0 4.758499210618914 0.0 68-69 0.0 1.0379764441625762E-4 0.0 5.259738035505022 0.0 70-71 0.0 1.0379764441625762E-4 0.0 5.8296909009946924 0.0 72-73 0.0 1.0379764441625762E-4 0.0 6.437426109051881 0.0 74-75 0.0 1.0379764441625762E-4 0.0 7.0676854059473975 0.0 76-77 0.0 1.0379764441625762E-4 0.0 7.625442048218158 0.0 78-79 0.0 1.0379764441625762E-4 0.0 8.322495129295536 0.0 80-81 0.0 1.0379764441625762E-4 0.0 9.081255909978378 0.0 82-83 0.0 1.0379764441625762E-4 0.0 9.826782490998148 0.0 84-85 0.0 1.0379764441625762E-4 0.0 10.715186529556899 5.189882220812881E-5 86-87 0.0 1.0379764441625762E-4 0.0 11.66036787961134 1.0379764441625762E-4 88-89 0.0 1.0379764441625762E-4 0.0 12.485818646831628 1.0379764441625762E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGCG 635 0.0 31.387972 2 CTCCGAC 190 0.0 31.220806 94-95 TTTGCGT 655 0.0 30.429562 3 ATGGGCG 95 3.5125995E-5 29.971973 5 GTAAGCG 225 0.0 29.527945 94-95 GTGCTCG 85 6.441527E-4 27.915073 9 TTGCGTT 780 0.0 26.76984 4 GGACAAT 330 0.0 24.446838 6 GCTTTGC 845 0.0 24.24212 1 TGCTATA 200 2.073648E-8 23.727814 2 GAGTACT 6485 0.0 22.941154 12-13 AGGCTTA 290 3.6379788E-12 22.909615 6 TTAGACT 150 3.054518E-5 22.145958 4 TCACTAG 55 0.0028902316 21.575218 32-33 CCCTATA 155 3.933826E-5 21.431574 2 AGTACTT 6680 0.0 21.205845 12-13 GTACTTT 7175 0.0 20.701897 14-15 GTACATG 7990 0.0 20.689096 1 TAGGACA 460 0.0 20.632881 4 TACATGG 8050 0.0 20.456028 2 >>END_MODULE