##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938966_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 970513 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.03733901555157 33.0 31.0 34.0 30.0 34.0 2 32.246038950534405 34.0 31.0 34.0 30.0 34.0 3 32.31914461733125 34.0 31.0 34.0 30.0 34.0 4 35.89646300461715 37.0 35.0 37.0 35.0 37.0 5 35.83356534121645 37.0 35.0 37.0 35.0 37.0 6 35.915198456898565 37.0 35.0 37.0 35.0 37.0 7 35.87151743459387 37.0 35.0 37.0 35.0 37.0 8 35.86886832015645 37.0 35.0 37.0 35.0 37.0 9 37.47487359777767 39.0 37.0 39.0 34.0 39.0 10-11 37.397333678168145 39.0 37.0 39.0 34.0 39.0 12-13 37.33449165544408 39.0 37.0 39.0 34.0 39.0 14-15 38.34798606510165 40.0 38.0 41.0 33.0 41.0 16-17 38.373643629709235 40.0 37.5 41.0 33.5 41.0 18-19 38.627790148096935 40.0 38.0 41.0 34.0 41.0 20-21 38.642402008010194 40.0 38.0 41.0 34.0 41.0 22-23 38.600638013092045 40.0 38.0 41.0 34.0 41.0 24-25 38.49320462477061 40.0 38.0 41.0 34.0 41.0 26-27 38.19365531425133 40.0 37.5 41.0 33.5 41.0 28-29 37.97808839242751 40.0 37.0 41.0 33.0 41.0 30-31 37.82218321650508 40.0 37.0 41.0 33.0 41.0 32-33 37.58931822654617 40.0 36.0 41.0 33.0 41.0 34-35 37.507229681621986 40.0 36.0 41.0 33.0 41.0 36-37 37.29255404100718 40.0 35.5 41.0 32.5 41.0 38-39 37.06261688406028 39.5 35.0 41.0 31.5 41.0 40-41 36.87178894048817 39.0 35.0 41.0 31.0 41.0 42-43 36.704538218447354 39.0 35.0 41.0 31.0 41.0 44-45 36.405552527374695 39.0 35.0 41.0 30.0 41.0 46-47 35.92144515323339 39.0 35.0 41.0 29.0 41.0 48-49 35.487878060366015 39.0 35.0 41.0 27.0 41.0 50-51 35.18654824819451 39.0 35.0 40.0 26.0 41.0 52-53 34.85988235088041 38.0 35.0 40.0 24.5 41.0 54-55 34.65395260032581 38.0 34.0 40.0 24.0 41.0 56-57 34.44091578371439 38.0 34.0 40.0 23.5 41.0 58-59 34.191622368788465 38.0 34.0 40.0 23.0 41.0 60-61 33.68853173527815 37.5 33.0 40.0 22.0 41.0 62-63 33.338757955843974 37.0 33.0 40.0 22.0 41.0 64-65 32.9861088929257 36.0 32.5 39.0 20.0 40.5 66-67 32.492168574764065 36.0 32.0 39.0 18.0 40.0 68-69 32.093360418665185 35.0 32.0 38.5 16.0 40.0 70-71 31.553304798596205 35.0 31.0 37.5 12.0 39.5 72-73 30.97913577664596 35.0 31.0 37.0 7.5 39.0 74-75 30.524883231857793 34.5 30.5 36.5 6.0 39.0 76-77 28.512686589463513 32.5 27.5 34.5 2.0 36.0 78-79 29.4409456648185 34.0 29.0 35.0 2.0 37.0 80-81 29.49312992201032 34.0 29.5 35.0 2.0 37.0 82-83 29.184156214290795 34.0 29.0 35.0 2.0 36.0 84-85 28.815497577054607 34.0 29.0 35.0 2.0 36.0 86-87 28.27557384599691 34.0 29.0 35.0 2.0 35.5 88-89 27.641607067602393 33.0 26.5 35.0 2.0 35.0 90-91 27.097942531424103 33.0 25.5 35.0 2.0 35.0 92-93 26.82628414044943 33.0 25.0 35.0 2.0 35.0 94-95 26.476888511539773 33.0 25.0 35.0 2.0 35.0 96-97 25.792710144016617 32.0 24.0 34.0 2.0 35.0 98-99 24.94913978483544 32.0 17.5 34.0 2.0 35.0 100-101 20.85643726565229 27.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.037207952912957865 1101 2 0.08876099325356535 1101 3 0.13979146674659404 1101 4 0.1961205361075642 1101 5 0.14387118073244665 1101 6 0.060288963269009344 1101 7 0.1031464515310816 1101 8 0.0706242723886561 1101 9 0.054814364547851824 1101 10-11 0.22090722497173942 1101 12-13 0.12515571018448668 1101 14-15 0.31164815691970915 1101 16-17 0.332188870486533 1101 18-19 0.14334497576356142 1101 20-21 0.1434174207401142 1101 22-23 0.1745042096672691 1101 24-25 0.1466881501677335 1101 26-27 0.1313209895941867 1101 28-29 0.26922410198174873 1101 30-31 0.38205543564107813 1101 32-33 0.4056035144993828 1101 34-35 0.49258843739730196 1101 36-37 0.43451080715623647 1101 38-39 0.35052902329749713 1101 40-41 0.5561163603987112 1101 42-43 0.6737979294298597 1101 44-45 0.5829342503680479 1101 46-47 0.4748186976402522 1101 48-49 0.2855011232590243 1101 50-51 0.3916551098468659 1101 52-53 0.5728929912228509 1101 54-55 0.8045060956064134 1101 56-57 0.534028526029843 1101 58-59 0.5641414450204465 1101 60-61 0.5750896639709993 1101 62-63 0.48571721627291 1101 64-65 0.539052481163047 1101 66-67 0.6971303998043794 1101 68-69 0.8045190628588728 1101 70-71 0.6039657676103296 1101 72-73 0.3925631556361715 1101 74-75 0.43249999124305205 1101 76-77 0.4176783636469885 1101 78-79 0.7567251025272483 1101 80-81 1.032186087000106 1101 82-83 0.6601424110161815 1101 84-85 0.7041725713107567 1101 86-87 0.6880643936006585 1101 88-89 0.8040795965523309 1101 90-91 0.8013728445819801 1101 92-93 1.084846334284773 1101 94-95 0.8670288167658278 1101 96-97 0.581766653273398 1101 98-99 0.4555533570692276 1101 100-101 1.2313504263505521 1102 1 0.17670626796032707 1102 2 0.12552359607634855 1102 3 0.12128431825084363 1102 4 0.15364778073013952 1102 5 0.1285941074017387 1102 6 0.14729633231494432 1102 7 0.10003078280637112 1102 8 0.07827933442872137 1102 9 0.28569418820939063 1102 10-11 0.309323064416283 1102 12-13 0.30902783429259273 1102 14-15 0.3438885273931902 1102 16-17 0.180377244504065 1102 18-19 0.08371697466017025 1102 20-21 0.2651121868997066 1102 22-23 0.2936534325297373 1102 24-25 0.3466083198960348 1102 26-27 0.22431880697354245 1102 28-29 0.14560984999012305 1102 30-31 0.1943805685750064 1102 32-33 0.1810274856994667 1102 34-35 0.2412438439278759 1102 36-37 0.3043982693544365 1102 38-39 0.3610347124755364 1102 40-41 0.49079918125475075 1102 42-43 0.3689365455441376 1102 44-45 0.5501955762878481 1102 46-47 0.9304201005174448 1102 48-49 0.6840887121941464 1102 50-51 0.510955603447087 1102 52-53 0.6332589138662996 1102 54-55 0.7118845482974479 1102 56-57 0.5813177925447306 1102 58-59 0.4544205840229978 1102 60-61 0.5188150427516618 1102 62-63 0.3555169668668654 1102 64-65 0.3934582096206256 1102 66-67 0.4844570899370737 1102 68-69 0.5267783612423145 1102 70-71 0.49876268982285055 1102 72-73 0.5118935220291512 1102 74-75 0.33893967553212434 1102 76-77 0.12737837724863965 1102 78-79 0.03145818594607164 1102 80-81 0.2571466485577609 1102 82-83 0.28697339075480244 1102 84-85 0.19185099768830582 1102 86-87 0.36587551873715185 1102 88-89 0.4614825195097616 1102 90-91 0.6839111167142242 1102 92-93 0.7441267940461209 1102 94-95 0.5950891572648196 1102 96-97 0.2584540529294159 1102 98-99 0.2681314553545313 1102 100-101 1.2738238173193075 1103 1 0.006760480132662394 1103 2 -0.014648659524915786 1103 3 0.02700249388052356 1103 4 -0.00502148062284391 1103 5 -0.07913741655718098 1103 6 -0.18300575625955418 1103 7 -0.018228458913817747 1103 8 -0.059718761485875405 1103 9 -0.08203970352411716 1103 10-11 0.09741957096219522 1103 12-13 0.03786029944191682 1103 14-15 -0.026663262743056748 1103 16-17 -0.04370857252614968 1103 18-19 -0.06802839584722165 1103 20-21 -0.0502316090375885 1103 22-23 0.09179818248288285 1103 24-25 0.011227231508982527 1103 26-27 0.03722885127066178 1103 28-29 -0.007618462674514603 1103 30-31 -0.01602518413066889 1103 32-33 -0.08510482850422108 1103 34-35 -0.22435374784805617 1103 36-37 -0.1733816459976225 1103 38-39 0.07259413868482767 1103 40-41 -0.04006266403090564 1103 42-43 -0.11427938563254969 1103 44-45 -0.25685545912024565 1103 46-47 -0.3093787372998591 1103 48-49 -0.3897260439744059 1103 50-51 -0.578514212359778 1103 52-53 -0.13982812158126023 1103 54-55 -0.06306587642212236 1103 56-57 -0.12170716281285365 1103 58-59 -0.22033474593626323 1103 60-61 -0.09904833665462576 1103 62-63 -0.022790518260549675 1103 64-65 0.08569807505211458 1103 66-67 -0.0668206450892157 1103 68-69 -0.16763813030769015 1103 70-71 -0.14813120921129652 1103 72-73 -0.04097876119615407 1103 74-75 -0.24863592060446038 1103 76-77 -0.30249726525685716 1103 78-79 -0.25269079640414205 1103 80-81 -0.05001414306820706 1103 82-83 0.05050300273573782 1103 84-85 0.18451459611373267 1103 86-87 0.047185391370611285 1103 88-89 0.04180865579492021 1103 90-91 0.3574400134299225 1103 92-93 0.1416280519263715 1103 94-95 0.050402344782700226 1103 96-97 0.25984697032367876 1103 98-99 0.1258978793243024 1103 100-101 0.8232289139541074 1104 1 0.10764180901703924 1104 2 0.12226318340752584 1104 3 0.05318847966320561 1104 4 0.08247428803134937 1104 5 0.10186384075699806 1104 6 0.16530170541653888 1104 7 0.12652346332613718 1104 8 0.10739642783661907 1104 9 -0.033394943139185784 1104 10-11 0.05247784263877264 1104 12-13 0.09272211577732037 1104 14-15 -0.029119861224607746 1104 16-17 0.22958498615656708 1104 18-19 0.15441163993786944 1104 20-21 0.22861347534753662 1104 22-23 0.17435778401100777 1104 24-25 0.0428891832474676 1104 26-27 0.08601626845717902 1104 28-29 0.027447304528926963 1104 30-31 -0.0723208222176197 1104 32-33 0.07353255724943608 1104 34-35 0.0036632017143034545 1104 36-37 0.2198651260900064 1104 38-39 0.4322283086156773 1104 40-41 0.28866273371560425 1104 42-43 -0.06836082090504902 1104 44-45 0.26583422606762497 1104 46-47 0.4045798680485575 1104 48-49 0.363930443283202 1104 50-51 0.270697548363664 1104 52-53 0.06445762724604975 1104 54-55 -0.009816202840227106 1104 56-57 -0.08247956476652973 1104 58-59 0.07594771453088356 1104 60-61 0.10923301157304621 1104 62-63 0.01713766975627351 1104 64-65 0.05272863745841505 1104 66-67 -0.032387766323317635 1104 68-69 -2.3883633795662718E-4 1104 70-71 -0.10947423837131254 1104 72-73 -0.28128958376977664 1104 74-75 -0.30275120664343547 1104 76-77 -0.32959843069038897 1104 78-79 -0.13078572362499585 1104 80-81 0.1045907701287554 1104 82-83 0.263265950653917 1104 84-85 0.04581228942578974 1104 86-87 0.10292067837675489 1104 88-89 0.14830014474506115 1104 90-91 0.20602855736337844 1104 92-93 0.12065145731348181 1104 94-95 0.13433290235318296 1104 96-97 0.020540790140884724 1104 98-99 0.2483149449464328 1104 100-101 0.6615552353429557 1105 1 -0.04553992904043014 1105 2 -0.0592104636927715 1105 3 0.05759531907797566 1105 4 -0.05609437052673627 1105 5 -0.048832439870537314 1105 6 0.07009634669981324 1105 7 0.09297860170089223 1105 8 0.08169574036967475 1105 9 -0.0665696302535892 1105 10-11 0.03850816169394733 1105 12-13 0.013487143099730758 1105 14-15 0.200908342547649 1105 16-17 0.07478153119446063 1105 18-19 -0.0629601210351538 1105 20-21 0.057610478696737744 1105 22-23 0.06350814537183425 1105 24-25 0.08084969796630759 1105 26-27 0.19824084099375483 1105 28-29 -0.057401981563089066 1105 30-31 0.08580538966322138 1105 32-33 -0.006953957821949075 1105 34-35 0.23268664609999234 1105 36-37 0.15514628244466877 1105 38-39 0.3676769248680998 1105 40-41 0.32072689729747594 1105 42-43 0.162592819144308 1105 44-45 0.1815402017418677 1105 46-47 0.19429430485448052 1105 48-49 0.0866168525904456 1105 50-51 -0.05234140809676546 1105 52-53 0.11256268629770005 1105 54-55 0.00661249449804302 1105 56-57 -0.059026365401109615 1105 58-59 -0.057914439532218864 1105 60-61 -0.17812817298538874 1105 62-63 -0.16317257173852084 1105 64-65 -0.0975710276217896 1105 66-67 -0.32325679505591154 1105 68-69 -0.10806538313859448 1105 70-71 -0.11708044320121047 1105 72-73 -0.013265610563362173 1105 74-75 0.09707251977997444 1105 76-77 -0.06087817685644126 1105 78-79 0.2639877648290856 1105 80-81 0.33268877823734044 1105 82-83 0.31195271250831524 1105 84-85 0.06068977929006181 1105 86-87 0.041119162423992606 1105 88-89 0.1668264976447631 1105 90-91 0.16090252175611752 1105 92-93 0.2925534492048918 1105 94-95 0.5659651956724154 1105 96-97 0.4602287859103207 1105 98-99 0.7534445060252217 1105 100-101 1.109669108073433 1106 1 -0.06484876694509012 1106 2 -0.15655391720320466 1106 3 -0.1177942502394913 1106 4 0.033463446735069624 1106 5 -0.06376545119822907 1106 6 0.03904005321467707 1106 7 0.029986249334726267 1106 8 0.02090567654355624 1106 9 -0.010011640488770013 1106 10-11 0.05213302512905926 1106 12-13 0.04788999514964587 1106 14-15 -0.0775316551523133 1106 16-17 0.012875947846758606 1106 18-19 -0.12388531222600108 1106 20-21 0.002898271233100047 1106 22-23 0.10578001012946459 1106 24-25 0.22353556996907287 1106 26-27 0.1505351311438332 1106 28-29 0.18194308197552544 1106 30-31 0.10975743382399372 1106 32-33 0.1587696923419344 1106 34-35 0.2499973240368547 1106 36-37 0.1851934332698093 1106 38-39 0.32932950378823733 1106 40-41 0.47450183835894677 1106 42-43 0.3229573755240409 1106 44-45 0.3267704932719724 1106 46-47 0.284343755719874 1106 48-49 0.3537320271060054 1106 50-51 0.4262394670685552 1106 52-53 0.49527788381365667 1106 54-55 0.5160714100290562 1106 56-57 0.5846312736075703 1106 58-59 0.6693640378411345 1106 60-61 0.5721622070962624 1106 62-63 0.3723055491285905 1106 64-65 0.21596758430391816 1106 66-67 0.20378592819174202 1106 68-69 0.20078977182661362 1106 70-71 0.263682595151959 1106 72-73 0.4615086979870391 1106 74-75 0.46118614176836203 1106 76-77 -0.0038803043936681547 1106 78-79 0.39775523075974206 1106 80-81 0.5504105883690151 1106 82-83 0.45225516780128316 1106 84-85 0.45235213338125746 1106 86-87 0.3159682222825424 1106 88-89 0.4636006911931503 1106 90-91 0.4616902855419873 1106 92-93 0.535259446559845 1106 94-95 0.3816313448300228 1106 96-97 0.359271394155968 1106 98-99 0.4397880170366655 1106 100-101 0.9225119578233034 1107 1 -0.09515816343095906 1107 2 -0.1258816482956462 1107 3 -0.15117851303551788 1107 4 -0.2050010299647056 1107 5 -0.26777614638540115 1107 6 -0.1895313753567507 1107 7 -0.07776776136045527 1107 8 -0.09402938839150465 1107 9 0.049690422154469616 1107 10-11 -0.015533132242985914 1107 12-13 -0.11893582608794873 1107 14-15 -0.10686702566415107 1107 16-17 -0.043894487600141474 1107 18-19 -0.0446263359081982 1107 20-21 0.03756213677932152 1107 22-23 0.01435525841059615 1107 24-25 0.08075482130596612 1107 26-27 -0.16075211102576503 1107 28-29 -0.21205997379223618 1107 30-31 -0.18442705815461125 1107 32-33 -0.3324927217146012 1107 34-35 -0.5228636385299907 1107 36-37 -0.24147768972485295 1107 38-39 -0.2519252708488935 1107 40-41 -0.167056603199498 1107 42-43 -0.26709228070208013 1107 44-45 -0.4283574670030461 1107 46-47 -0.6455550219730242 1107 48-49 -0.3905766054379285 1107 50-51 -0.6857639706701804 1107 52-53 -0.5945903362016267 1107 54-55 -0.663645931452983 1107 56-57 -0.550194929600984 1107 58-59 -0.4438139606309832 1107 60-61 -0.5600665935148825 1107 62-63 -0.6918942216888198 1107 64-65 -0.7853884126403088 1107 66-67 -0.4985154469037454 1107 68-69 -0.3990001441397695 1107 70-71 -0.527732607292652 1107 72-73 -0.6686001637394696 1107 74-75 -0.800129748224002 1107 76-77 -0.9615197237977959 1107 78-79 -0.751012912861345 1107 80-81 -0.43621087839951045 1107 82-83 -0.44844192921934223 1107 84-85 -0.3222945434713047 1107 86-87 -0.44508983730493057 1107 88-89 -0.22431947687407572 1107 90-91 -0.04945371751378147 1107 92-93 0.03025562685014549 1107 94-95 0.04837122259931448 1107 96-97 -0.14674464862478231 1107 98-99 -0.11765086760810206 1107 100-101 0.42462234743369365 1108 1 0.04643962322468553 1108 2 -0.015441734625255776 1108 3 0.0455382064391614 1108 4 0.02782947928817947 1108 5 0.06142668228705617 1108 6 -0.057649114255589495 1108 7 -0.028667793504460803 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70-71 -2.006044884986192 2316 72-73 -1.879964009446386 2316 74-75 -2.026833625390381 2316 76-77 -2.029763378763999 2316 78-79 -2.0320357351437153 2316 80-81 -2.260752625958567 2316 82-83 -2.2396837632210733 2316 84-85 -2.5961734457910843 2316 86-87 -2.520622664263705 2316 88-89 -2.6702104386550793 2316 90-91 -2.7541802539968394 2316 92-93 -3.1802783337830114 2316 94-95 -3.298394085547553 2316 96-97 -3.6502537251052942 2316 98-99 -4.061954685256577 2316 100-101 -4.700353739122345 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 0.0 4 11.0 5 46.0 6 130.0 7 241.0 8 448.0 9 830.0 10 1442.0 11 2150.0 12 3281.0 13 4659.0 14 5346.0 15 7081.0 16 9621.0 17 13856.0 18 12910.0 19 7621.0 20 6582.0 21 6839.0 22 7156.0 23 7789.0 24 8921.0 25 10346.0 26 11973.0 27 14616.0 28 17467.0 29 21128.0 30 25382.0 31 30615.0 32 37789.0 33 47855.0 34 65030.0 35 95088.0 36 140666.0 37 201513.0 38 135660.0 39 8423.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 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20.991178692022906 18-19 18.227267434851466 30.70293751861129 32.282411466925225 18.78738357961202 20-21 20.91955491580226 27.378252532423573 30.175020839494167 21.527171712279998 22-23 19.697417757412833 26.928644953751263 30.210414492129424 23.163522796706484 24-25 21.583997329247524 27.240696415194847 31.138325813255463 20.036980442302163 26-27 22.251170257379343 24.76947758556557 33.67528307194236 19.30406908511272 28-29 19.24404928115337 27.34538331789476 33.69996074241149 19.71060665854038 30-31 19.47557631891587 28.55773183872859 31.86201524348463 20.104676598870906 32-33 19.368313458964487 28.56664465081869 31.327813228673907 20.737228661542918 34-35 21.23333968747907 26.04165915331867 31.377286848463925 21.347714310738333 36-37 21.930410741804202 25.867959398280078 32.93111505314794 19.27051480676778 38-39 22.426309381316717 25.54293808220703 33.178825247335745 18.851927289140516 40-41 19.894826162288375 27.022782856262594 33.62785854752529 19.45453243392374 42-43 18.567511620215 29.10277823859076 31.092099284188574 21.237610857005667 44-45 19.923040423612544 28.312396735941274 29.98806297104768 21.776499869398496 46-47 20.897537020426856 28.17720346435874 28.544638574607305 22.380620940607095 48-49 21.290433596321936 27.436062033299986 29.55128792976071 21.722216440617366 50-51 21.39384734592605 28.549584341396468 30.528393377488904 19.52817493518858 52-53 20.30426176671513 29.30120462064908 30.18068794544741 20.213845667188384 54-55 18.384297788901332 27.778607808447696 31.313027234050445 22.524067168600524 56-57 21.061438641213464 29.298886259122753 27.90962099425768 21.73005410540611 58-59 21.30138524819889 27.74221235801309 28.805104934302715 22.151297459485303 60-61 20.238476732959434 27.414880890434034 30.795173174870914 21.551469201735614 62-63 22.432053975577865 27.05991573528639 29.864360394966372 20.643669894169374 64-65 21.09341311257002 28.627790603202534 30.194438705775255 20.084357578452185 66-67 19.751828954147346 27.773776403915505 29.94049459041731 22.533900051519833 68-69 18.969362589148954 29.766619457154146 29.830452763096005 21.43356519060089 70-71 20.802297341715153 29.184462238012266 27.598960549729885 22.414279870542693 72-73 19.96227768200941 27.390668646375683 28.735524408225338 23.911529263389568 74-75 19.724980500003607 30.09712389220958 28.21507800513749 21.96281760264932 76-77 21.218936357995112 27.014284748955582 29.14242502866699 22.624353864382318 78-79 19.867997646605456 27.079235414672446 30.80041174100708 22.252355197715023 80-81 20.198905115129833 29.39775149843433 28.881220550368724 21.522122836067112 82-83 19.485777109631712 28.615690876886763 29.086524343311215 22.812007670170313 84-85 19.524364949258793 27.61050083821649 28.707961665634567 24.15717254689015 86-87 20.257909267408284 28.073352557345704 28.920065565955234 22.74867260929078 88-89 21.094693157326887 26.457370097360677 29.05136079854903 23.3965759467634 90-91 19.7475286318928 26.366153267209636 30.344637219082827 23.54168088181474 92-93 19.51478238828331 28.855718573579125 29.328458248369678 22.301040789767885 94-95 19.09206780331639 28.620327599939415 28.440062111481247 23.84754248526295 96-97 17.769092776897942 27.799221234998882 28.711936289233197 25.719749698869975 98-99 19.445283061638534 27.744296057858058 27.745996189644035 25.064424690859372 100-101 20.323283634377802 26.927800846977362 28.998001050993803 23.75091446765103 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5192.0 1 3303.5 2 1407.0 3 1369.0 4 1130.0 5 1110.0 6 1822.5 7 3295.5 8 4741.0 9 5008.0 10 3927.5 11 2827.5 12 2566.0 13 2564.5 14 2575.0 15 2529.0 16 2511.5 17 2615.0 18 2782.0 19 2973.5 20 3174.0 21 3405.0 22 3792.5 23 4234.0 24 4548.5 25 5077.5 26 6038.5 27 7169.0 28 8023.5 29 9152.5 30 10756.0 31 12575.5 32 15094.5 33 17579.5 34 19580.0 35 20796.5 36 23052.0 37 25908.5 38 25978.0 39 24909.0 40 24205.0 41 23511.0 42 22354.0 43 22369.0 44 23555.5 45 24413.0 46 28354.5 47 45679.0 48 108113.5 49 108955.5 50 49799.0 51 30883.5 52 20477.5 53 16856.5 54 15432.5 55 14147.5 56 13212.0 57 12050.5 58 11227.0 59 10651.5 60 9581.5 61 8277.5 62 6708.0 63 4664.5 64 2984.0 65 2096.5 66 1433.5 67 1057.0 68 836.5 69 575.5 70 400.5 71 302.0 72 238.0 73 207.0 74 146.0 75 109.5 76 79.5 77 36.0 78 14.0 79 7.5 80 6.0 81 3.0 82 1.5 83 0.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.40236452268027323 2 0.0 3 0.0 4 0.0 5 3.091148701769064E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001391016915796079 16-17 0.002266842381297314 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0018546892210614385 36-37 0.0063368548386265825 38-39 0.012519152242164709 40-41 0.0025244381064447358 42-43 6.182297403538128E-4 44-45 3.606340152063908E-4 46-47 1.545574350884532E-4 48-49 2.0607658011793763E-4 50-51 5.151914502948441E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.0303829005896881E-4 60-61 0.0020607658011793763 62-63 0.0 64-65 1.545574350884532E-4 66-67 0.0013394977707665947 68-69 0.0017001317859729856 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.1228731608953203 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14554158470829345 88-89 0.43899463479623657 90-91 0.3333288683407641 92-93 0.0 94-95 0.0 96-97 2.0607658011793763E-4 98-99 0.0 100-101 3.091148701769064E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 970513.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.042880827959312 #Duplication Level Percentage of deduplicated Percentage of total 1 68.05054942678468 19.763839972792624 2 13.89001858185809 8.068123087420897 3 5.9509071575909225 5.184944621884897 4 3.3054008884591966 3.8399345636860502 5 2.02692624984647 2.9433888760676754 6 1.3194162866836152 2.2991789985972497 7 0.9558271276267802 1.943198135185666 8 0.6620381405323281 1.5381995855235344 9 0.5042906733182118 1.3181448535048965 >10 2.9215211321348242 15.821973972154732 >50 0.24551421829913772 4.902745841518551 >100 0.1405479748661878 7.130129166748618 >500 0.006404717842003167 1.2535105409316072 >1k 0.016011794605007918 11.233128549860398 >5k 0.0032023589210015834 6.434859676279492 >10k+ 0.0014232706315562595 6.324699557843129 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 19241 1.982559739024619 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 16390 1.6887975740664987 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 14893 1.5345492538482224 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 10679 1.100345899539728 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8959 0.9231200406383016 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7961 0.8202878271594507 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7870 0.8109113427640846 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 7580 0.7810302386469836 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 6574 0.677373718847661 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 6334 0.6526445292335085 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 5875 0.6053499540964418 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5818 0.5994767715630805 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5298 0.5458968607324167 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 4783 0.49283214135204784 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 4522 0.465939147646657 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 4493 0.4629510372349469 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4456 0.45913862050276505 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4449 0.45841735247235227 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4078 0.4201901468604748 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4005 0.4126683516861701 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4003 0.41246227510605216 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3870 0.3987581825282093 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3636 0.37464722265441064 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 3506 0.3612522449467447 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3431 0.353524373192322 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 3324 0.34249927615601233 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3236 0.33343190663082306 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3096 0.31900654602256745 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 3015 0.31066044452779096 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 2811 0.28964063335576135 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2774 0.2858282166235795 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2525 0.26017168239889626 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2183 0.2249325871987289 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2075 0.21380445187236027 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 2036 0.2097859585600605 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1998 0.2058705035378197 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1987 0.20473708234717103 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1950 0.2009246656149892 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1905 0.1962879425623356 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1808 0.1862932284266156 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1753 0.18062612247337234 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1691 0.17423774848971627 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1544 0.15909111985104785 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1456 0.1500237503258586 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1438 0.14816906110479713 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1380 0.14219284028137696 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1375 0.14167764883108214 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1216 0.12529456071170608 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1207 0.12436721610117535 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1161 0.1196274547584628 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1134 0.11684542092687063 No Hit GCTTTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1112 0.11457857854557332 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1089 0.11220869787421704 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 1063 0.10952970233268386 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 1062 0.10942666404262487 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1026 0.105717285600502 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1021 0.10520209415020715 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1018 0.10489297928003025 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.0303829005896881E-4 0.0 12-13 0.0 0.0 0.0 2.0607658011793763E-4 0.0 14-15 0.0 0.0 0.0 2.0607658011793763E-4 0.0 16-17 0.0 0.0 0.0 4.636723052653597E-4 0.0 18-19 0.0 0.0 0.0 5.667105953243285E-4 0.0 20-21 0.0 0.0 0.0 8.243063204717505E-4 0.0 22-23 0.0 0.0 0.0 0.001545574350884532 0.0 24-25 0.0 0.0 0.0 0.003400263571945971 0.0 26-27 0.0 0.0 0.0 0.005873182533361223 0.0 28-29 0.0 0.0 0.0 0.01931967938605665 0.0 30-31 0.0 0.0 0.0 0.05239497049498564 0.0 32-33 0.0 0.0 0.0 0.10236854117358551 0.0 34-35 0.0 0.0 0.0 0.1902602025938859 0.0 36-37 0.0 0.0 0.0 0.31421526553482537 0.0 38-39 0.0 0.0 0.0 0.4744913257215514 0.0 40-41 0.0 0.0 0.0 0.6634635496897002 0.0 42-43 0.0 0.0 0.0 0.8492931058110504 0.0 44-45 0.0 0.0 0.0 1.052484613807337 0.0 46-47 0.0 0.0 0.0 1.2949337103160907 0.0 48-49 0.0 0.0 0.0 1.5732916509103947 0.0 50-51 0.0 0.0 0.0 1.8677750838989278 0.0 52-53 0.0 0.0 0.0 2.1176429372919268 0.0 54-55 0.0 0.0 0.0 2.376526641065086 0.0 56-57 0.0 0.0 0.0 2.6985728166443934 0.0 58-59 0.0 0.0 0.0 3.0773930900461917 0.0 60-61 0.0 0.0 0.0 3.589596429929326 0.0 62-63 0.0 0.0 0.0 4.036988685365369 0.0 64-65 0.0 0.0 0.0 4.418127320293494 0.0 66-67 0.0 0.0 0.0 4.851454849136488 0.0 68-69 0.0 0.0 0.0 5.345729526549361 0.0 70-71 0.0 0.0 0.0 5.926762444191887 0.0 72-73 0.0 0.0 0.0 6.548804601277881 0.0 74-75 0.0 0.0 0.0 7.166261554456252 0.0 76-77 0.0 0.0 0.0 7.726326180071776 0.0 78-79 0.0 0.0 0.0 8.430747450059918 0.0 80-81 0.0 0.0 0.0 9.191324588130195 0.0 82-83 0.0 0.0 0.0 9.92737861316644 0.0 84-85 0.0 0.0 0.0 10.81149866101742 0.0 86-87 0.0 0.0 0.0 11.762490559116674 0.0 88-89 0.0 0.0 0.0 12.588548530519425 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAGTAC 45 9.673881E-4 42.17819 3 CATATAG 210 0.0 36.152733 3 CGTCACT 95 3.4223383E-5 30.086517 1 TTTGCGT 620 0.0 27.551882 3 CTTTGCG 670 0.0 25.495771 2 GCTAGAT 100 0.0016635327 23.725231 2 ACATATA 380 0.0 23.725231 2 GAGTACT 6335 0.0 23.446766 12-13 TACCTGG 490 0.0 22.272667 2 GACATAT 345 0.0 22.092516 1 AGTACTT 6585 0.0 22.052145 12-13 GTACATG 7600 0.0 22.000767 1 GTCGGGA 260 6.330083E-10 21.900215 2 GTATAGT 240 5.7316356E-9 21.83362 1 GTACTTT 6880 0.0 21.451475 14-15 GCTTATG 245 7.2686817E-9 21.388035 1 TACATGG 7740 0.0 21.334316 2 GCTTTGC 850 0.0 21.296535 1 CATGGGG 1440 0.0 20.759579 4 ACATGGG 7680 0.0 20.759579 3 >>END_MODULE