FastQCFastQC Report
Fri 27 May 2016
SRR938965_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938965_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3016522
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114000.37791867587904215No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT94690.3139045563068991No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90790.30097575950051086No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58880.1951916810154211No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT54220.17974342636983917No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT41860.13876908572190091No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC36220.12007205649420094No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG117500.023.63421
TACATGG119150.023.1365972
GAGTACT101500.021.75995312-13
ACATGGG121700.021.367433
GTACTTT105650.020.99640314-15
AGTACTT103950.019.85794812-13
GTATCAA257250.019.8554531
CATGGGG67250.019.7213334
ATGGGGA36650.017.8351045
TTTGCGT5600.017.7624053
AGAGTAC189300.017.7191810-11
ACTTTTT128400.017.66622416-17
TATCAAC289550.017.3892252
ATCAACG288950.017.3761543
TCAACGC289150.017.3477554
CAACGCA291100.017.1990055
TACTTTT120300.017.16033214-15
AACGCAG294300.017.0196176
GGTATCA206400.016.927431
GTCTTAG12450.016.7766881
Produced by FastQC (version 0.11.5)