##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938965_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3016522 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.55683300171522 33.0 31.0 34.0 30.0 34.0 2 31.646332763361247 33.0 31.0 34.0 30.0 34.0 3 31.68623567141231 33.0 31.0 34.0 28.0 34.0 4 35.22468624462212 37.0 35.0 37.0 33.0 37.0 5 35.25321048545312 37.0 35.0 37.0 33.0 37.0 6 35.3298530559366 37.0 35.0 37.0 33.0 37.0 7 35.24289496313967 37.0 35.0 37.0 32.0 37.0 8 35.22293124333255 37.0 35.0 37.0 32.0 37.0 9 36.784559502632504 39.0 37.0 39.0 32.0 39.0 10-11 36.65714919367404 39.0 37.0 39.0 32.0 39.0 12-13 36.52618959848461 39.0 37.0 39.0 32.0 39.0 14-15 37.67049220923965 40.0 37.5 41.0 32.0 41.0 16-17 37.57544201567235 40.0 37.0 41.0 32.0 41.0 18-19 37.47152880038667 40.0 37.0 41.0 32.0 41.0 20-21 37.594212805343375 40.0 37.0 41.0 32.0 41.0 22-23 37.51918815775254 40.0 37.0 41.0 32.0 41.0 24-25 37.41912440883905 40.0 37.0 41.0 31.5 41.0 26-27 37.279873476805406 40.0 37.0 41.0 31.0 41.0 28-29 37.138911799748186 40.0 37.0 41.0 31.0 41.0 30-31 36.935657024878324 40.0 36.5 41.0 30.0 41.0 32-33 36.71207221429182 39.5 36.0 41.0 30.0 41.0 34-35 36.37229332323782 39.0 35.5 41.0 30.0 41.0 36-37 36.08652680139578 39.0 35.0 41.0 29.0 41.0 38-39 35.83018174573233 39.0 35.0 40.5 27.5 41.0 40-41 35.664732430262404 39.0 35.0 40.0 27.0 41.0 42-43 35.436120472517686 39.0 35.0 40.0 26.5 41.0 44-45 35.34950267228285 39.0 35.0 40.5 25.5 41.0 46-47 35.11076149950175 39.0 35.0 41.0 24.5 41.0 48-49 34.85034884545844 39.0 34.0 41.0 24.0 41.0 50-51 33.736767873730074 38.0 33.0 39.5 22.5 40.5 52-53 33.83940544773087 38.0 33.5 39.5 22.5 40.5 54-55 34.31299042407116 38.0 34.0 40.0 23.0 41.0 56-57 34.19109159488974 38.0 34.0 40.0 22.0 41.0 58-59 33.951095997310816 38.0 34.0 40.0 21.5 41.0 60-61 33.611059690597315 37.5 33.0 40.0 20.0 41.0 62-63 33.21005084663729 37.0 33.0 40.0 18.5 41.0 64-65 32.792780891370924 36.0 32.0 39.5 16.5 41.0 66-67 32.359524313099655 36.0 32.0 39.0 13.0 41.0 68-69 31.90136223107274 35.0 31.0 39.0 9.5 40.0 70-71 31.431941984842148 35.0 31.0 38.0 7.5 40.0 72-73 30.978456149167815 35.0 30.5 37.0 6.5 39.5 74-75 30.50210673086422 35.0 30.0 37.0 2.0 39.0 76-77 30.059002221764004 34.5 30.0 36.0 2.0 39.0 78-79 29.642890719842256 34.0 29.0 36.0 2.0 38.5 80-81 29.173000893081504 34.0 29.0 35.0 2.0 37.0 82-83 28.694281195363402 34.0 29.0 35.0 2.0 37.0 84-85 28.265646330442806 34.0 27.5 35.0 2.0 36.0 86-87 27.797746543867408 34.0 26.5 35.0 2.0 36.0 88-89 27.296671298933006 33.5 26.0 35.0 2.0 35.5 90-91 26.808073005931995 33.0 25.0 35.0 2.0 35.0 92-93 26.266829646858202 33.0 24.5 35.0 2.0 35.0 94-95 25.68142930832263 32.5 21.5 35.0 2.0 35.0 96-97 24.976752034296453 32.0 18.0 35.0 2.0 35.0 98-99 24.04694877080293 32.0 2.0 34.0 2.0 35.0 100-101 20.83586859303529 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13452405385611854 1101 2 0.29876268765141134 1101 3 0.3343699127814652 1101 4 0.28498078801038673 1101 5 0.211723839258525 1101 6 0.3135896841586856 1101 7 0.2992339540040305 1101 8 0.1636285157082824 1101 9 0.32339485596263273 1101 10-11 0.28380992976573793 1101 12-13 0.2682692899718191 1101 14-15 0.42748999854787684 1101 16-17 0.3948480311209366 1101 18-19 0.4180450766915129 1101 20-21 0.29709064640655214 1101 22-23 0.4875015520035646 1101 24-25 0.38414156564825674 1101 26-27 0.5372152847791085 1101 28-29 0.4416438430245293 1101 30-31 0.34879863082714735 1101 32-33 0.3637044318049121 1101 34-35 0.22725192060057253 1101 36-37 0.26941764896074005 1101 38-39 0.5059772074971818 1101 40-41 0.29443086481170155 1101 42-43 0.33497247569012245 1101 44-45 0.4011853070334226 1101 46-47 0.23356035307333656 1101 48-49 0.20401438569825103 1101 50-51 0.2767293806470974 1101 52-53 0.2710389595024978 1101 54-55 0.22805132621595448 1101 56-57 0.2749900486453072 1101 58-59 0.1911440882828117 1101 60-61 0.21316251202848235 1101 62-63 0.2860334532884323 1101 64-65 0.16738614076326996 1101 66-67 0.20619594989200607 1101 68-69 0.1682412544492422 1101 70-71 0.10525605889247203 1101 72-73 0.10172983137352176 1101 74-75 -0.13769228973375292 1101 76-77 -0.20329694402378706 1101 78-79 -0.2255804234590819 1101 80-81 -0.3315675786289667 1101 82-83 -0.1994136695599451 1101 84-85 -0.20181294447124287 1101 86-87 -0.3684454086868314 1101 88-89 -0.4512454270047286 1101 90-91 -0.28489125056415787 1101 92-93 -0.2670399738456197 1101 94-95 -0.4938182904617143 1101 96-97 -0.5813874479387309 1101 98-99 -0.7330707910033141 1101 100-101 -0.44562962276765106 1102 1 0.38504655676771904 1102 2 0.40037569760280434 1102 3 0.4524656346607223 1102 4 0.21176911665270381 1102 5 0.37212311274863197 1102 6 0.368961658176822 1102 7 0.4150197689462942 1102 8 0.4672519573513654 1102 9 0.45632784903693846 1102 10-11 0.54156035817158 1102 12-13 0.4883749151902421 1102 14-15 0.625432367363608 1102 16-17 0.6680888121870012 1102 18-19 0.6053154792131181 1102 20-21 0.6493254140660341 1102 22-23 0.6286284751124214 1102 24-25 0.6914759919165405 1102 26-27 0.6173683132832224 1102 28-29 0.6112167630956051 1102 30-31 0.7148334633482065 1102 32-33 0.8028332514477583 1102 34-35 0.7870556401520616 1102 36-37 0.6865347361582863 1102 38-39 0.8250859069725252 1102 40-41 0.732399321662534 1102 42-43 0.6624349947391224 1102 44-45 0.8791058322460259 1102 46-47 0.82351476128202 1102 48-49 0.6755059299192183 1102 50-51 0.5530680687075105 1102 52-53 0.6325748702401128 1102 54-55 0.7285272208866118 1102 56-57 0.7336497969130065 1102 58-59 0.67459048905733 1102 60-61 0.826559859044643 1102 62-63 0.6864591922083179 1102 64-65 0.49124397468395387 1102 66-67 0.6444043039505942 1102 68-69 0.7100515512349261 1102 70-71 0.5872525722689694 1102 72-73 0.44915690551572496 1102 74-75 0.2714112620783844 1102 76-77 0.416639771923073 1102 78-79 0.591273971893024 1102 80-81 0.5253351836232127 1102 82-83 0.4909450792912047 1102 84-85 0.39320289226681737 1102 86-87 0.44635369532791813 1102 88-89 0.2767249772025657 1102 90-91 0.421820134854169 1102 92-93 0.3232830231510775 1102 94-95 0.10232998186325304 1102 96-97 0.05163127662329359 1102 98-99 -0.0712776103236088 1102 100-101 0.20310872171266325 1103 1 0.3021820272726359 1103 2 0.2669620923118643 1103 3 0.34700654886306026 1103 4 0.34493123782277024 1103 5 0.273428770719228 1103 6 0.3002897360975041 1103 7 0.3214758626457481 1103 8 0.2752819502225705 1103 9 0.3842296796648128 1103 10-11 0.3274547596566393 1103 12-13 0.3934568643482308 1103 14-15 0.5084308511676312 1103 16-17 0.4563229369960382 1103 18-19 0.5593979452822708 1103 20-21 0.5765957202255692 1103 22-23 0.5147506833476712 1103 24-25 0.5503723578174942 1103 26-27 0.5847949156726244 1103 28-29 0.565369391443646 1103 30-31 0.609713420613808 1103 32-33 0.589914079800117 1103 34-35 0.5680382344064796 1103 36-37 0.5877595508102686 1103 38-39 0.794701610838338 1103 40-41 0.6347935502682489 1103 42-43 0.5370598631928374 1103 44-45 0.6483388350981798 1103 46-47 0.6508449452223246 1103 48-49 0.5843096138162096 1103 50-51 0.46674806296661586 1103 52-53 0.5547948354442198 1103 54-55 0.6174705027748573 1103 56-57 0.4412501305707792 1103 58-59 0.5038264391089484 1103 60-61 0.6362882011899345 1103 62-63 0.6261115736132794 1103 64-65 0.6859439788156649 1103 66-67 0.6872965453072908 1103 68-69 0.5691699164704573 1103 70-71 0.566752906834953 1103 72-73 0.5644791320174676 1103 74-75 0.4650293472404208 1103 76-77 0.518349940695014 1103 78-79 0.42355657659823365 1103 80-81 0.5152680798312623 1103 82-83 0.6643100583493329 1103 84-85 0.5643132125688126 1103 86-87 0.7056088162468797 1103 88-89 0.7386905622530016 1103 90-91 0.9647716016167536 1103 92-93 0.7431835398191886 1103 94-95 0.7116842573683293 1103 96-97 0.5947870902236723 1103 98-99 0.6626227689620308 1103 100-101 0.8313703562750234 1104 1 0.27478886349144105 1104 2 0.21005061135780778 1104 3 0.2630794836199648 1104 4 0.2026658572947042 1104 5 0.250645984487079 1104 6 0.19159822666258464 1104 7 0.20789738853379447 1104 8 0.17880049374013396 1104 9 0.2518730964344158 1104 10-11 0.32552213881851344 1104 12-13 0.2724250015110883 1104 14-15 0.3751022798695587 1104 16-17 0.35565562614356594 1104 18-19 0.4586824854320639 1104 20-21 0.43228353151296517 1104 22-23 0.47017086487002757 1104 24-25 0.4073529601785779 1104 26-27 0.3852324815840973 1104 28-29 0.46160912600120696 1104 30-31 0.37334872196182545 1104 32-33 0.4282907721834661 1104 34-35 0.553128031335703 1104 36-37 0.48446942004799354 1104 38-39 0.4805200118500821 1104 40-41 0.48698881469061917 1104 42-43 0.4892305265857928 1104 44-45 0.42594102516073207 1104 46-47 0.4935995170299208 1104 48-49 0.45251248514585285 1104 50-51 0.34246750668646087 1104 52-53 0.3516058112334619 1104 54-55 0.48971928553773836 1104 56-57 0.5124037551816514 1104 58-59 0.34885949253949633 1104 60-61 0.47550137932321235 1104 62-63 0.5387925732448338 1104 64-65 0.5224629065102064 1104 66-67 0.4906726591414241 1104 68-69 0.4500874853863266 1104 70-71 0.34546556884142277 1104 72-73 0.41580422542271833 1104 74-75 0.389402560208449 1104 76-77 0.26265319741963467 1104 78-79 0.28862817713942945 1104 80-81 0.43449727971799135 1104 82-83 0.7207850898904127 1104 84-85 0.4989895498041399 1104 86-87 0.4806715431625115 1104 88-89 0.5118185748958233 1104 90-91 0.5218136367702186 1104 92-93 0.6068108607404419 1104 94-95 0.83325140511052 1104 96-97 0.6719640973580994 1104 98-99 0.7799168993264267 1104 100-101 0.7232043623832851 1105 1 0.19069726244183016 1105 2 0.23277156330846083 1105 3 0.30814263993281443 1105 4 0.2825628135546694 1105 5 0.11730060767165185 1105 6 0.2791118518173761 1105 7 0.3827864356170636 1105 8 0.3086024764502113 1105 9 0.2522992942038442 1105 10-11 0.33132990679128227 1105 12-13 0.3146050409154739 1105 14-15 0.3466940150970643 1105 16-17 0.3683276300000884 1105 18-19 0.4784775665194161 1105 20-21 0.5116345667319777 1105 22-23 0.4296604253422913 1105 24-25 0.47682441639414463 1105 26-27 0.45812132666865324 1105 28-29 0.3475213258452712 1105 30-31 0.42770924721703096 1105 32-33 0.41011040001792054 1105 34-35 0.4947136870850031 1105 36-37 0.5122845575583668 1105 38-39 0.5099244254731943 1105 40-41 0.5723099731288457 1105 42-43 0.5284027246343115 1105 44-45 0.5810076676591152 1105 46-47 0.5902039991569339 1105 48-49 0.5406781538542944 1105 50-51 0.5583988256599994 1105 52-53 0.5453401429153502 1105 54-55 0.6564930708862917 1105 56-57 0.5746391807792861 1105 58-59 0.5394355206592749 1105 60-61 0.718220765051953 1105 62-63 0.5770510198649674 1105 64-65 0.627909955523684 1105 66-67 0.6696316875568868 1105 68-69 0.5802363789310476 1105 70-71 0.5326499458510447 1105 72-73 0.4628957244971339 1105 74-75 0.4797962527742179 1105 76-77 0.4983464799377657 1105 78-79 0.45379975650589444 1105 80-81 0.42560811650985997 1105 82-83 0.5346623039475737 1105 84-85 0.623790966527487 1105 86-87 0.5275019442616973 1105 88-89 0.5391332898678307 1105 90-91 0.6209758544233424 1105 92-93 0.5898307707875858 1105 94-95 0.7436598523649458 1105 96-97 0.7945977482945636 1105 98-99 0.7510707267141328 1105 100-101 0.783378144706738 1106 1 0.275611550101555 1106 2 0.2033057963492837 1106 3 0.2641335432442098 1106 4 0.214306204470887 1106 5 0.17927883245556586 1106 6 0.26775907757086514 1106 7 0.1891500984173149 1106 8 0.23784484541933182 1106 9 0.30418429897135724 1106 10-11 0.2898536312133473 1106 12-13 0.27488965879710037 1106 14-15 0.3828204655940368 1106 16-17 0.39545178457380814 1106 18-19 0.5956418211387486 1106 20-21 0.48201961559275475 1106 22-23 0.6011789075126615 1106 24-25 0.5440905229069202 1106 26-27 0.5530785349243246 1106 28-29 0.4051934828522761 1106 30-31 0.3380858462480276 1106 32-33 0.43567769591398076 1106 34-35 0.36165641147184857 1106 36-37 0.38317215141614014 1106 38-39 0.5195502332487862 1106 40-41 0.606016377243968 1106 42-43 0.5094386018928461 1106 44-45 0.5242323889199483 1106 46-47 0.55416419842539 1106 48-49 0.6106558187837905 1106 50-51 0.3516391864351007 1106 52-53 0.5238926709961405 1106 54-55 0.5595979459463152 1106 56-57 0.542499624298415 1106 58-59 0.6325719017158562 1106 60-61 0.7941455237388553 1106 62-63 0.7816415187477403 1106 64-65 0.5905894996300631 1106 66-67 0.7053005374508814 1106 68-69 0.7603834872995208 1106 70-71 0.606341315699904 1106 72-73 0.6912753076845704 1106 74-75 0.7203524922547793 1106 76-77 0.504589045848924 1106 78-79 0.763107028868216 1106 80-81 0.8624488897047478 1106 82-83 0.9464403713197171 1106 84-85 0.956731164476686 1106 86-87 0.9560256994190119 1106 88-89 1.0568522211984224 1106 90-91 0.9566464065773985 1106 92-93 0.9990556844983693 1106 94-95 1.0909202539422758 1106 96-97 1.0453817927273334 1106 98-99 1.1840174027085553 1106 100-101 1.3638657878354863 1107 1 0.16456376587257537 1107 2 0.1972615829526312 1107 3 0.2570424267330509 1107 4 0.25568055754736463 1107 5 0.2822410001826583 1107 6 0.1825924078125709 1107 7 0.2089348282397907 1107 8 0.16871608213305933 1107 9 0.11034583971594714 1107 10-11 0.22900722801536233 1107 12-13 0.3602393916802953 1107 14-15 0.35099472684911603 1107 16-17 0.1565729563633056 1107 18-19 0.22917622634010826 1107 20-21 0.291088370346138 1107 22-23 0.3144539404385114 1107 24-25 0.3311016203118058 1107 26-27 0.20894142423779982 1107 28-29 0.2688735965624218 1107 30-31 0.39623603916572137 1107 32-33 0.32941865289704 1107 34-35 0.29758385401390086 1107 36-37 0.2425268046280351 1107 38-39 0.4180118769446395 1107 40-41 0.2442714691581216 1107 42-43 0.40514096606209904 1107 44-45 0.2544179794521355 1107 46-47 0.38457934246442704 1107 48-49 0.45221532183523294 1107 50-51 0.3748803196410577 1107 52-53 0.5575366358257625 1107 54-55 0.5859437323641785 1107 56-57 0.44594397032597755 1107 58-59 0.5157489918121385 1107 60-61 0.38678497169745896 1107 62-63 0.32998629884011876 1107 64-65 0.34472957828112527 1107 66-67 0.4086527142673688 1107 68-69 0.48805500318741224 1107 70-71 0.3651281138226672 1107 72-73 0.37167619158151055 1107 74-75 0.42061045010968456 1107 76-77 0.4124397757827012 1107 78-79 0.5116719716421478 1107 80-81 0.39300304114513906 1107 82-83 0.5546105052491157 1107 84-85 0.5790489824662615 1107 86-87 0.6901075549533395 1107 88-89 0.6311504598969719 1107 90-91 0.6433563019566506 1107 92-93 0.7376636859452503 1107 94-95 0.7185449316398795 1107 96-97 0.7784653223791551 1107 98-99 0.7665522067301467 1107 100-101 0.902583812655088 1108 1 0.2466300394418326 1108 2 0.2926194232552284 1108 3 0.2962683503065726 1108 4 0.2772628378283528 1108 5 0.18421695456566312 1108 6 0.2428012487123965 1108 7 0.32221205671343967 1108 8 0.41602131879729143 1108 9 0.3904316559776646 1108 10-11 0.44364510993445805 1108 12-13 0.4957693029489789 1108 14-15 0.5329231279791102 1108 16-17 0.5339929820536682 1108 18-19 0.5182510056968042 1108 20-21 0.3797508358632129 1108 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68-69 -2.072366944622466 2316 70-71 -1.7721183913031489 2316 72-73 -1.840310993879811 2316 74-75 -1.9574652721643133 2316 76-77 -1.9821855737047187 2316 78-79 -1.8553527254687552 2316 80-81 -1.8479016652854305 2316 82-83 -1.7990136792802396 2316 84-85 -1.7408599178133244 2316 86-87 -1.7566471048789047 2316 88-89 -1.9335939483043312 2316 90-91 -2.195175617894236 2316 92-93 -2.090468420298926 2316 94-95 -2.1599655529826727 2316 96-97 -2.3177769888976414 2316 98-99 -2.2974941586007027 2316 100-101 -1.9818858984353156 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11701.0 3 3929.0 4 4010.0 5 4362.0 6 5257.0 7 6308.0 8 7595.0 9 9100.0 10 11048.0 11 13492.0 12 16581.0 13 21627.0 14 19534.0 15 20303.0 16 24422.0 17 30658.0 18 27569.0 19 21868.0 20 22515.0 21 24231.0 22 26329.0 23 28827.0 24 32779.0 25 36998.0 26 42542.0 27 48297.0 28 56613.0 29 66383.0 30 79078.0 31 93814.0 32 115607.0 33 146027.0 34 195180.0 35 273391.0 36 403673.0 37 563974.0 38 442556.0 39 58332.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.68042517739283 18.710964847368167 14.593854816421082 23.014755158817923 2 15.26720545950792 20.243286269143812 40.32326243290251 24.16624583844576 3 19.780270465958814 24.17004964803057 29.624099171294 26.425580714716617 4 13.053518759905597 16.389166164473387 36.73532777071315 33.82198730490787 5 14.140500958480025 37.04442329239217 33.78399816269203 15.031077586435782 6 32.18922585502901 35.37125059290635 17.319101369572213 15.12042218249243 7 28.657600732934867 32.676204063449816 20.51919094552114 18.147004258094178 8 26.07659287648368 34.69079539419761 19.610086379526585 19.622525349792134 9 26.334707861146928 17.417437938095496 20.599672727260664 35.64818147349691 10-11 25.268302164926638 26.195542638101728 26.817263288064012 21.718891908907626 12-13 26.516958288415292 23.624048428559583 27.400199021477732 22.45879426154739 14-15 23.214834790309823 24.953769812641283 26.215426868404425 25.615968528644473 16-17 22.44596076495794 28.172563996929767 26.69448319829744 22.686992039814857 18-19 22.33802120739157 27.102718015999255 28.69381973551301 21.86544104109616 20-21 23.252026398077003 25.949041653203793 28.557898557022774 22.24103339169643 22-23 22.890662649852896 25.93975277019971 28.614273900359905 22.555310679587492 24-25 23.02122323039788 26.009477562750323 28.609643900397163 22.359655306454634 26-27 22.776437502218894 25.94129980148585 28.95816404773763 22.324098648557626 28-29 22.317617285578844 26.590520934684715 28.784264542549128 22.30759723718732 30-31 22.599265557875928 26.139945446845342 28.540576210438555 22.720212784840175 32-33 22.453996013449505 26.318116909731685 28.63518949384543 22.59269758297338 34-35 23.09394953338351 26.185082173789993 28.35758178218533 22.36338651064117 36-37 22.87794485910769 26.0138584348734 28.641470592595763 22.466726113423146 38-39 22.704068292829152 26.323964607900773 28.666467934445013 22.30549916482506 40-41 22.794270928726277 26.298448515283408 28.493155018598504 22.41412553739181 42-43 22.526760824707583 26.44302091648944 28.004858079229926 23.02536017957305 44-45 22.734709392506677 26.31133499816105 28.051858406136233 22.902097203196043 46-47 22.98778512411836 26.590219937722203 27.737920248111802 22.68407469004763 48-49 23.052951820696745 26.567702344710764 27.611998729905302 22.76734710468719 50-51 22.645412865891423 26.826446099262665 27.85137687487235 22.67676415997356 52-53 22.98000482092528 26.994782772671716 27.274309815580448 22.750902590822548 54-55 22.679958171303767 26.59371963246981 27.50938030833173 23.216941887894695 56-57 22.849231099222855 26.87367418697623 27.388861521920944 22.88823319187997 58-59 23.26837621182695 26.515283205856733 27.337404059537036 22.878936522779284 60-61 22.95702319722753 26.527915598268905 27.963765629010133 22.551295575493434 62-63 22.82405878336998 26.5440552990326 27.919269301805077 22.712616615792342 64-65 22.612224566582565 26.81726289590235 27.68004118225731 22.890471355257777 66-67 22.680690286804214 26.72174747986869 27.348258546486992 23.249303686840104 68-69 22.410079824437894 26.74842679788279 27.716434536212603 23.125058841466718 70-71 22.778560554961622 26.605185702593253 27.450685037417788 23.16556870502734 72-73 22.745389765475117 26.435160155511326 27.62791906546587 23.191531013547678 74-75 22.453793256499534 27.1153203424299 27.244626249115715 23.186260151954848 76-77 22.73230533473805 26.50200921950671 27.299750565288182 23.465934880467053 78-79 22.616424118674573 26.639128655768367 27.474361722164854 23.270085503392206 80-81 22.731484796956895 26.722231567236637 27.311283910605404 23.23499972520106 82-83 22.611267908255765 26.594735224726072 27.52710129225091 23.266895574767254 84-85 22.60207142626336 26.568635191194357 27.324608172243316 23.504685210298966 86-87 22.536671376463925 26.561041287705095 27.504560863733403 23.397726472097574 88-89 22.595507736631383 26.483712197179326 27.127731874068445 23.79304819212085 90-91 22.557177232929128 26.33126443966278 27.379266593409085 23.732291733999002 92-93 22.681378688959953 26.462247128786508 27.32825116799502 23.52812301425852 94-95 22.567407659678835 26.246158214098987 27.369710848440278 23.816723277781907 96-97 22.291673226637798 26.127377895821162 27.604841666870755 23.976107210670285 98-99 22.3640782700171 26.214001313541573 27.228997080019074 24.192923336422258 100-101 22.642539037826516 25.340956336800698 27.813418644876375 24.20308598049641 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3191.0 1 1879.5 2 661.5 3 771.5 4 717.0 5 748.5 6 1257.0 7 2486.0 8 3947.5 9 4608.0 10 4192.0 11 3679.5 12 3805.5 13 4095.5 14 4338.0 15 4666.5 16 5014.5 17 5191.0 18 5340.5 19 5602.5 20 5980.0 21 6697.0 22 7736.5 23 8942.0 24 10319.5 25 12440.5 26 15368.0 27 18980.0 28 22209.0 29 25539.5 30 30010.0 31 35167.0 32 39748.5 33 43783.5 34 49001.0 35 53944.0 36 59074.5 37 64833.5 38 70146.0 39 73966.0 40 76901.5 41 81453.0 42 86198.0 43 91215.5 44 97432.5 45 102400.0 46 106158.0 47 116256.0 48 138727.5 49 140784.0 50 123251.0 51 115081.0 52 110569.0 53 109145.5 54 107194.5 55 102001.5 56 94673.0 57 85729.5 58 77845.0 59 69113.5 60 59015.5 61 48366.5 62 38669.5 63 31343.0 64 24141.5 65 17976.0 66 13385.5 67 10229.5 68 7328.5 69 4813.5 70 3325.5 71 2324.0 72 1570.0 73 1042.0 74 749.0 75 519.0 76 343.0 77 213.5 78 145.0 79 92.0 80 57.0 81 41.0 82 32.0 83 31.0 84 29.0 85 22.0 86 15.5 87 10.5 88 15.5 89 14.0 90 8.5 91 10.5 92 7.5 93 4.0 94 5.0 95 5.0 96 4.0 97 2.0 98 1.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32411499070784167 2 0.08698759697426374 3 0.09640241311019777 4 0.1019054394431733 5 0.11330930124162861 6 0.05675410290394037 7 0.059439314548344085 8 0.059770822158764295 9 0.06335110435130259 10-11 0.060466988140646744 12-13 0.058743148566461637 14-15 0.05691985670915048 16-17 0.058610545522293556 18-19 0.06653357741133664 20-21 0.06829056774656375 22-23 0.08368909625058261 24-25 0.0919104849890039 26-27 0.08721965230155788 28-29 0.08501512669226348 30-31 0.07947894959824593 32-33 0.07082660096627838 34-35 0.32033580394905126 36-37 0.49581935752499073 38-39 0.4693153240718947 40-41 0.45058514408315276 42-43 0.48110041962233324 44-45 0.4909627710323346 46-47 0.4856420738850902 48-49 0.5029301957685042 50-51 0.49895210444346166 52-53 0.4980404585148061 54-55 0.48913947917502343 56-57 0.4680555951522979 58-59 0.45144706387024525 60-61 0.4115003968146097 62-63 0.4812330226665013 64-65 0.5048695152894624 66-67 0.515079949690405 68-69 0.5262517561615663 70-71 0.5241135320743558 72-73 0.5123284365239172 74-75 0.5138036453902872 76-77 0.48845988857366196 78-79 0.4683042258601131 80-81 0.4749509534490383 82-83 0.48365302822256895 84-85 0.4849624832837287 86-87 0.4847801540979977 88-89 0.49155948473109096 90-91 0.5052175982804037 92-93 0.511814599727766 94-95 0.5184447519361702 96-97 0.5093780187911774 98-99 0.5130080271252787 100-101 0.49122797712067073 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3016522.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.67550961047574 #Duplication Level Percentage of deduplicated Percentage of total 1 73.52013851138533 37.991906242086 2 13.992236290040196 14.461118817560395 3 5.080167162449093 7.875606810778844 4 2.429911862972922 5.02267735310665 5 1.3276041237307168 3.4302309827376947 6 0.8122751840981484 2.5184840449328894 7 0.5363076613638117 1.93997801962842 8 0.3803979508523789 1.5725806372061908 9 0.30066299923141315 1.3983222335667618 >10 1.4914643241956924 13.674835558080728 >50 0.08582667562526554 3.0372652672924048 >100 0.039468447463485413 3.6072588462951276 >500 0.0018015742649356138 0.6758529183679001 >1k 0.001415522636432705 1.4220940211119975 >5k 2.5736775207867363E-4 0.9927493694877841 >10k+ 6.434193801966841E-5 0.3790388777600891 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11400 0.37791867587904215 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9469 0.3139045563068991 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9079 0.30097575950051086 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5888 0.1951916810154211 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5422 0.17974342636983917 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4186 0.13876908572190091 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3622 0.12007205649420094 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.3150761042021245E-5 0.0 0.0 0.0 0.0 3 3.3150761042021245E-5 0.0 0.0 0.0 0.0 4 3.3150761042021245E-5 0.0 0.0 0.0 0.0 5 3.3150761042021245E-5 0.0 0.0 0.0 0.0 6 6.630152208404249E-5 0.0 0.0 0.0 0.0 7 2.9835684937819115E-4 0.0 0.0 0.0 0.0 8 3.315076104202124E-4 0.0 0.0 0.0 0.0 9 3.315076104202124E-4 0.0 0.0 0.0 0.0 10-11 3.315076104202124E-4 0.0 0.0 6.630152208404249E-5 0.0 12-13 3.315076104202124E-4 0.0 0.0 9.945228312606373E-5 1.6575380521010622E-5 14-15 3.6465837146223366E-4 0.0 0.0 1.1602766364707435E-4 6.630152208404249E-5 16-17 3.8123375198324426E-4 0.0 0.0 1.491784246890956E-4 9.945228312606373E-5 18-19 4.4753527406728676E-4 0.0 0.0 1.9890456625212745E-4 1.3260304416808498E-4 20-21 4.972614156303186E-4 0.0 0.0 3.8123375198324426E-4 1.3260304416808498E-4 22-23 5.967136987563823E-4 0.0 0.0 6.795906013614355E-4 1.3260304416808498E-4 24-25 6.961659818824461E-4 0.0 0.0 9.116459286555842E-4 1.3260304416808498E-4 26-27 7.127413624034567E-4 0.0 0.0 0.001226578158554786 1.3260304416808498E-4 28-29 8.619197870925522E-4 0.0 0.0 0.002983568493781912 1.491784246890956E-4 30-31 8.950705481345735E-4 0.0 0.0 0.007690976561748927 1.657538052101062E-4 32-33 9.282213091765948E-4 0.0 0.0 0.01705606655611993 1.657538052101062E-4 34-35 0.0011602766364707433 0.0 0.0 0.030846783149600765 1.9890456625212745E-4 36-37 0.0013923319637648922 0.0 0.0 0.05464902957777201 1.9890456625212745E-4 38-39 0.0014586334858489346 0.0 0.0 0.08869486116792782 1.9890456625212745E-4 40-41 0.0014586334858489346 0.0 0.0 0.12587343967655465 1.9890456625212745E-4 42-43 0.0015415103884539878 0.0 0.0 0.16754394630637537 2.1547994677313805E-4 44-45 0.001657538052101062 0.0 0.0 0.21982269646964286 2.486307078151593E-4 46-47 0.0018067164767901576 0.0 0.0 0.2830743485378194 2.6520608833616996E-4 48-49 0.0018564426183531894 0.0 0.0 0.34916039067508875 2.9835684937819115E-4 50-51 0.0018564426183531894 0.0 0.0 0.4222412433922246 2.9835684937819115E-4 52-53 0.0018564426183531894 0.0 0.0 0.4975266217186548 2.9835684937819115E-4 54-55 0.0018564426183531894 0.0 0.0 0.5849617539669858 3.315076104202124E-4 56-57 0.0019558949014792533 3.3150761042021245E-5 0.0 0.6914088476729161 3.6465837146223366E-4 58-59 0.0019558949014792533 3.3150761042021245E-5 0.0 0.811547205689201 3.978091325042549E-4 60-61 0.0019558949014792533 3.3150761042021245E-5 0.0 0.9476973812887823 4.4753527406728676E-4 62-63 0.0019890456625212744 3.3150761042021245E-5 0.0 1.083035363242834 4.6411065458829736E-4 64-65 0.0019890456625212744 3.3150761042021245E-5 0.0 1.2253515803962312 4.6411065458829736E-4 66-67 0.0021547994677313807 3.3150761042021245E-5 0.0 1.3939729264364722 4.6411065458829736E-4 68-69 0.002187950228773402 3.3150761042021245E-5 0.0 1.5932753018211039 4.6411065458829736E-4 70-71 0.0022376763703364335 3.3150761042021245E-5 0.0 1.8115564879022927 4.6411065458829736E-4 72-73 0.0023371286534624976 3.3150761042021245E-5 0.0 2.039501120827231 4.972614156303186E-4 74-75 0.0023868547950255292 3.3150761042021245E-5 0.0 2.281717156380759 4.972614156303186E-4 76-77 0.0023868547950255292 3.3150761042021245E-5 0.0 2.5402599417474825 4.972614156303186E-4 78-79 0.0024200055560675508 3.3150761042021245E-5 0.0 2.844650229635322 4.972614156303186E-4 80-81 0.0026520608833616992 3.3150761042021245E-5 0.0 3.1796386699649464 5.138367961513293E-4 82-83 0.0026520608833616992 3.3150761042021245E-5 0.0 3.5187212292832606 5.635629377143611E-4 84-85 0.0027680885470087735 3.3150761042021245E-5 0.0 3.880661238340049 5.967136987563823E-4 86-87 0.0028509654496138266 3.3150761042021245E-5 0.0 4.260254027651713 6.298644597984035E-4 88-89 0.0028675408301348376 3.3150761042021245E-5 0.0 4.674787719101667 6.630152208404248E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11750 0.0 23.6342 1 TACATGG 11915 0.0 23.136597 2 GAGTACT 10150 0.0 21.759953 12-13 ACATGGG 12170 0.0 21.36743 3 GTACTTT 10565 0.0 20.996403 14-15 AGTACTT 10395 0.0 19.857948 12-13 GTATCAA 25725 0.0 19.855453 1 CATGGGG 6725 0.0 19.721333 4 ATGGGGA 3665 0.0 17.835104 5 TTTGCGT 560 0.0 17.762405 3 AGAGTAC 18930 0.0 17.71918 10-11 ACTTTTT 12840 0.0 17.666224 16-17 TATCAAC 28955 0.0 17.389225 2 ATCAACG 28895 0.0 17.376154 3 TCAACGC 28915 0.0 17.347755 4 CAACGCA 29110 0.0 17.199005 5 TACTTTT 12030 0.0 17.160332 14-15 AACGCAG 29430 0.0 17.019617 6 GGTATCA 20640 0.0 16.92743 1 GTCTTAG 1245 0.0 16.776688 1 >>END_MODULE