FastQCFastQC Report
Fri 27 May 2016
SRR938965_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938965_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3016522
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117460.3893888391995815No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT103590.3434087336342981No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100760.33402706825940603No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62950.2086840407595237No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT49020.16250503062798813No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT36510.12103342856441955No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC32810.10876764697887169No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT31200.10343037445110627No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG118250.021.3108081
GAGTACT107750.020.96583412-13
TACATGG120950.020.5195522
GTACTTT109750.020.38917514-15
AGTACTT109450.019.75127412-13
ACATGGG121000.019.4521853
GTATAGA7750.019.0548441
CATGGGG67250.017.9936814
GTATCAA281750.017.1273781
TACTTTT125550.017.02944814-15
AGAGTAC204850.016.93558910-11
ACTTTTT132700.016.8442216-17
TTTGCGT5700.016.65053
ACCGTGG9150.016.5959078
GGTATCA233250.016.4202521
GTAAGCG3200.015.57133394-95
TATCAAC317950.015.1488382
ATCAACG317350.015.1027133
TCAACGC317550.015.0932014
CAACGCA317950.015.0891385
Produced by FastQC (version 0.11.5)