##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938965_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3016522 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1675353271085 33.0 31.0 34.0 30.0 34.0 2 32.40641274951749 34.0 31.0 34.0 31.0 34.0 3 32.497858792344296 34.0 31.0 34.0 30.0 34.0 4 36.04788594281759 37.0 35.0 37.0 35.0 37.0 5 35.97004132573871 37.0 35.0 37.0 35.0 37.0 6 36.013003717526345 37.0 35.0 37.0 35.0 37.0 7 35.94979350390947 37.0 35.0 37.0 35.0 37.0 8 35.91721326746498 37.0 35.0 37.0 35.0 37.0 9 37.58880492169459 39.0 37.0 39.0 35.0 39.0 10-11 37.4979544322899 39.0 37.0 39.0 34.5 39.0 12-13 37.40006686508502 39.0 37.0 39.0 34.0 39.0 14-15 38.699256793088196 40.0 38.0 41.0 34.0 41.0 16-17 38.79856420738851 40.0 38.0 41.0 34.5 41.0 18-19 38.83392380363876 40.0 38.0 41.0 35.0 41.0 20-21 38.80374517407796 40.0 38.0 41.0 34.0 41.0 22-23 38.730502214139335 40.0 38.0 41.0 34.0 41.0 24-25 38.64711230350716 40.0 38.0 41.0 34.0 41.0 26-27 38.47953785850062 40.0 38.0 41.0 34.0 41.0 28-29 38.31392146319503 40.0 38.0 41.0 34.0 41.0 30-31 38.12062733174166 40.0 37.5 41.0 33.0 41.0 32-33 38.020822158764304 40.0 37.0 41.0 33.0 41.0 34-35 37.920528675076795 40.0 37.0 41.0 33.0 41.0 36-37 37.78568629700032 40.0 37.0 41.0 33.0 41.0 38-39 37.638360336838254 40.0 37.0 41.0 33.0 41.0 40-41 37.463912413037264 40.0 37.0 41.0 32.0 41.0 42-43 37.26708656525628 40.0 36.5 41.0 31.5 41.0 44-45 37.001032811960265 40.0 36.0 41.0 31.0 41.0 46-47 36.67203438264332 39.5 36.0 41.0 30.5 41.0 48-49 36.316743587482534 39.0 35.0 41.0 30.0 41.0 50-51 36.03629842580296 39.0 35.0 40.5 29.0 41.0 52-53 35.75328988152581 39.0 35.0 40.0 28.0 41.0 54-55 35.437935642438546 38.0 35.0 40.0 28.0 41.0 56-57 35.27865502058331 38.0 34.5 40.0 27.5 41.0 58-59 34.97548302316376 38.0 34.0 40.0 27.0 41.0 60-61 34.57760825215264 37.5 34.0 40.0 26.0 41.0 62-63 34.137343768750895 37.0 33.5 40.0 26.0 41.0 64-65 33.70088466120917 36.0 33.0 39.0 25.0 41.0 66-67 33.22260586861292 36.0 32.5 39.0 23.5 40.0 68-69 32.71788122214922 35.0 32.0 38.5 22.5 40.0 70-71 32.16715293307988 35.0 31.0 37.5 21.5 40.0 72-73 31.632632548345413 35.0 31.0 37.0 20.0 39.0 74-75 31.095295840706616 34.5 30.5 36.5 18.0 39.0 76-77 29.22470414603308 32.5 28.0 35.0 13.0 36.5 78-79 30.183003637964518 34.0 29.5 35.0 14.5 37.0 80-81 30.154751233374064 34.0 30.0 35.0 12.0 37.0 82-83 29.72834144753461 34.0 29.5 35.0 7.5 36.5 84-85 29.252713887052707 34.0 29.0 35.0 4.0 36.0 86-87 28.53382521327542 33.5 28.5 35.0 2.0 36.0 88-89 27.814178050085495 33.0 26.5 35.0 2.0 35.0 90-91 27.191867488451933 32.5 25.5 34.5 2.0 35.0 92-93 26.74263638720354 32.0 25.0 34.0 2.0 35.0 94-95 26.138672119745856 32.0 24.5 34.0 2.0 35.0 96-97 25.211964640072242 32.0 21.5 34.0 2.0 35.0 98-99 23.915159412064625 31.0 4.5 34.0 2.0 35.0 100-101 18.32881295080891 24.0 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08281895435403186 1101 2 0.10540765445635003 1101 3 0.14546727381061686 1101 4 0.094304043782941 1101 5 0.08568137469690384 1101 6 0.12666122287416925 1101 7 0.13716779722918204 1101 8 0.1293435852968301 1101 9 0.1414558203194929 1101 10-11 0.20786067459427215 1101 12-13 0.14456263828743232 1101 14-15 0.19307342730363786 1101 16-17 0.10850346187028492 1101 18-19 0.1610596453101678 1101 20-21 0.23151883770169235 1101 22-23 0.24993333867668355 1101 24-25 0.2629652851703028 1101 26-27 0.1170652985326015 1101 28-29 0.22179893549863294 1101 30-31 0.17790312819043663 1101 32-33 0.33948741011354855 1101 34-35 0.3056123321902149 1101 36-37 0.4181782760735189 1101 38-39 0.43209924502048835 1101 40-41 0.4336347658946522 1101 42-43 0.3998452154371108 1101 44-45 0.3656754689085844 1101 46-47 0.33887589576760746 1101 48-49 0.2570656092376211 1101 50-51 0.16414223686147977 1101 52-53 0.1657071132048955 1101 54-55 0.230106241264032 1101 56-57 0.23719860344932187 1101 58-59 0.32210755549727565 1101 60-61 0.15232963736684724 1101 62-63 0.22409270102082957 1101 64-65 0.38177418760571413 1101 66-67 0.09081141084246269 1101 68-69 0.24381505563820127 1101 70-71 0.3099489114902738 1101 72-73 0.2900046878564808 1101 74-75 0.27441590928862425 1101 76-77 0.09083039262078785 1101 78-79 0.48729052748287316 1101 80-81 0.3892101905113243 1101 82-83 0.41879908840187596 1101 84-85 0.5516301163608084 1101 86-87 0.6776538870664268 1101 88-89 0.6871540513846135 1101 90-91 0.7371870905806297 1101 92-93 0.7336808952717568 1101 94-95 0.5089643186524775 1101 96-97 0.8982834368047676 1101 98-99 0.8953686644613263 1101 100-101 1.249780982627449 1102 1 0.17450661045390348 1102 2 0.16271465671241714 1102 3 0.13701124318227187 1102 4 0.04134374746271163 1102 5 0.0955516289275593 1102 6 0.13118505270540481 1102 7 0.1606665275477397 1102 8 0.15299780221674553 1102 9 0.20947969883464168 1102 10-11 0.26414355353185215 1102 12-13 0.24896748816109238 1102 14-15 0.33427117481101476 1102 16-17 0.22166223255999995 1102 18-19 0.265907179124099 1102 20-21 0.2659666048964837 1102 22-23 0.15751988973982378 1102 24-25 0.17994644738988086 1102 26-27 0.22992328567551112 1102 28-29 0.24558744201963378 1102 30-31 0.260547842061122 1102 32-33 0.21067544096631252 1102 34-35 0.18471759989008518 1102 36-37 0.19204408560142383 1102 38-39 0.24523749802033734 1102 40-41 0.28017288690700326 1102 42-43 0.3847499320904504 1102 44-45 0.43076774567775544 1102 46-47 0.5623602725091317 1102 48-49 0.4777415406916887 1102 50-51 0.46853053168923253 1102 52-53 0.3735152075153181 1102 54-55 0.3801903751143456 1102 56-57 0.44518841329021086 1102 58-59 0.4379367708258428 1102 60-61 0.4037816633346196 1102 62-63 0.6369232767313591 1102 64-65 0.42901651448031686 1102 66-67 0.35085293935123474 1102 68-69 0.33767052958425836 1102 70-71 0.480554760755318 1102 72-73 0.5252571107931168 1102 74-75 0.6223620897904532 1102 76-77 0.39798863848032795 1102 78-79 0.5735184778851625 1102 80-81 0.6633323139148537 1102 82-83 0.6337801972830057 1102 84-85 0.8808411677848547 1102 86-87 1.011914164592401 1102 88-89 0.8664685970052126 1102 90-91 0.9867650375486292 1102 92-93 1.1685479227780426 1102 94-95 1.1142981883894265 1102 96-97 1.1718928066588319 1102 98-99 1.2971911410307833 1102 100-101 1.1619404877271826 1103 1 0.09552780483462442 1103 2 0.14116249313671148 1103 3 0.08451642732403997 1103 4 0.10476174924868786 1103 5 0.10874188152583741 1103 6 0.11956843065393485 1103 7 0.21665628193626674 1103 8 0.1851891767718996 1103 9 0.16831957674587983 1103 10-11 0.18380141232748315 1103 12-13 0.16251208265318695 1103 14-15 0.22950578097037777 1103 16-17 0.13224785925753224 1103 18-19 0.12733021852332627 1103 20-21 0.16219800149935537 1103 22-23 0.2582423501499349 1103 24-25 0.15933894822549632 1103 26-27 0.15248721873366122 1103 28-29 0.1457357741592915 1103 30-31 0.19339141241419355 1103 32-33 0.13043505745175565 1103 34-35 0.12589442789247585 1103 36-37 0.14835453980656155 1103 38-39 0.03822909862978463 1103 40-41 0.2088117732340038 1103 42-43 0.24803729260854368 1103 44-45 0.10137228357184114 1103 46-47 0.26377008021075454 1103 48-49 0.16452708626448498 1103 50-51 0.16070750794701638 1103 52-53 0.16841187069454833 1103 54-55 0.27628387757565775 1103 56-57 0.31768641171453993 1103 58-59 0.2141889436499227 1103 60-61 0.24708793249093475 1103 62-63 0.24253912437547598 1103 64-65 0.2869211426451841 1103 66-67 0.17482844536069564 1103 68-69 0.2091891981868983 1103 70-71 0.1569934488371061 1103 72-73 0.34178575753352547 1103 74-75 0.41152719192997367 1103 76-77 0.18701757335297842 1103 78-79 0.4176714912623005 1103 80-81 0.4741536398194661 1103 82-83 0.5705234344260042 1103 84-85 0.5855000582282166 1103 86-87 0.7191324112364121 1103 88-89 0.5550278347550517 1103 90-91 0.5979237052251527 1103 92-93 0.5609290923736374 1103 94-95 0.648857875638349 1103 96-97 0.5697162538051472 1103 98-99 1.0913627726753639 1103 100-101 1.0104989503021606 1104 1 0.0685535817399554 1104 2 0.06057223590560312 1104 3 0.05382426422999487 1104 4 0.05469389658994572 1104 5 0.036460910618743014 1104 6 0.10298766670506154 1104 7 0.08633400971972804 1104 8 0.11406505055884963 1104 9 0.12460688428944877 1104 10-11 0.08041136763709744 1104 12-13 0.1571060096118444 1104 14-15 0.17337191584542921 1104 16-17 0.16428889209984732 1104 18-19 0.17584594795717123 1104 20-21 0.26737304816391116 1104 22-23 0.14018005613122142 1104 24-25 0.13018926586931912 1104 26-27 0.14919925753097374 1104 28-29 0.1654273186116697 1104 30-31 0.1492331355570755 1104 32-33 0.21019282549005425 1104 34-35 0.28774649082645 1104 36-37 0.2366107645394493 1104 38-39 0.26037525234320213 1104 40-41 0.3488656620490218 1104 42-43 0.36847848967763497 1104 44-45 0.2665638823266505 1104 46-47 0.35412373618509463 1104 48-49 0.24557707443974408 1104 50-51 0.1899038802403865 1104 52-53 0.2571624631999967 1104 54-55 0.42178279000462027 1104 56-57 0.23606079243849365 1104 58-59 0.2752612846326201 1104 60-61 0.26784471801055787 1104 62-63 0.3396393494006702 1104 64-65 0.27696368885045786 1104 66-67 0.27249034525544147 1104 68-69 0.23866564666607815 1104 70-71 0.3213965205274789 1104 72-73 0.3418497658849411 1104 74-75 0.4281738673526938 1104 76-77 0.2820667398868508 1104 78-79 0.4750866652888881 1104 80-81 0.5556639933130434 1104 82-83 0.44200050103934885 1104 84-85 0.6972658082157821 1104 86-87 0.7550281551441351 1104 88-89 0.8166391653878122 1104 90-91 0.5856811723163595 1104 92-93 0.7161178795822174 1104 94-95 1.056118655685104 1104 96-97 0.8723361302189119 1104 98-99 0.5888604204160295 1104 100-101 0.9947126765347001 1105 1 -0.009928651643697606 1105 2 0.005067844141294131 1105 3 0.09630608190615675 1105 4 0.014407351168046034 1105 5 -0.01168321399982375 1105 6 -0.030404916331534082 1105 7 0.05459435190749673 1105 8 0.07424117489637183 1105 9 0.07139998393531499 1105 10-11 0.13231308306481537 1105 12-13 0.07574315990768099 1105 14-15 0.054175475511648585 1105 16-17 -0.021373846651414397 1105 18-19 0.05851366544001024 1105 20-21 0.05480758155176346 1105 22-23 0.12118260160777083 1105 24-25 0.052822233718345046 1105 26-27 -0.010081729510858395 1105 28-29 0.10683348093861156 1105 30-31 0.12603963347512348 1105 32-33 0.12972445439012148 1105 34-35 0.20055977180275875 1105 36-37 0.21645425700938148 1105 38-39 0.20984532286710333 1105 40-41 0.19116925489377934 1105 42-43 0.27150940963052506 1105 44-45 0.15518934206888702 1105 46-47 0.08858325300807479 1105 48-49 0.050305003152743666 1105 50-51 0.15343146331114355 1105 52-53 0.07507318659936146 1105 54-55 0.08750382782912425 1105 56-57 -0.041014658960321526 1105 58-59 -0.05968710323222837 1105 60-61 0.05045354359784682 1105 62-63 0.08856190094693517 1105 64-65 -0.14177250912389638 1105 66-67 0.09201742430524718 1105 68-69 0.11212186905593313 1105 70-71 0.15751243900196954 1105 72-73 0.06893578074306461 1105 74-75 0.1226686827190413 1105 76-77 -0.06037633697317446 1105 78-79 0.09163187250346638 1105 80-81 0.22026525565862798 1105 82-83 0.20636241442581493 1105 84-85 0.09910839998613952 1105 86-87 0.029781719152840225 1105 88-89 0.10410480365007757 1105 90-91 0.031240689236927466 1105 92-93 0.3486079943311502 1105 94-95 0.30990546068022695 1105 96-97 0.26882099885632016 1105 98-99 0.5797137576397056 1105 100-101 0.6873169864261008 1106 1 -0.05141745774705697 1106 2 -0.004094334193617044 1106 3 0.04047632588021344 1106 4 -0.004742719842205645 1106 5 0.044694338769645015 1106 6 -0.002250598891713196 1106 7 0.038044128497688234 1106 8 0.03338173216430107 1106 9 -0.005269690845530306 1106 10-11 0.11752534898337075 1106 12-13 0.0942854413286156 1106 14-15 -0.04142463144702191 1106 16-17 -0.06266263719800236 1106 18-19 -0.04171747546905635 1106 20-21 0.019753613997316677 1106 22-23 0.019559981314138497 1106 24-25 0.11684606677770404 1106 26-27 0.004683721513579542 1106 28-29 0.05883447581504697 1106 30-31 0.11746260703500155 1106 32-33 0.13781864185765613 1106 34-35 0.09258748506934467 1106 36-37 0.04200771022122751 1106 38-39 0.05618224836319996 1106 40-41 0.3115758420727843 1106 42-43 0.276525579918939 1106 44-45 0.32725810344535944 1106 46-47 0.29459330674510653 1106 48-49 0.41974614881159056 1106 50-51 0.4190675494275311 1106 52-53 0.4817549356053732 1106 54-55 0.4544814840108913 1106 56-57 0.5399538135284345 1106 58-59 0.511864738506759 1106 60-61 0.5673382492877224 1106 62-63 0.5921414346742679 1106 64-65 0.5439432356704401 1106 66-67 0.6050613466339527 1106 68-69 0.6013056613650676 1106 70-71 0.586963039296883 1106 72-73 0.6359896367049167 1106 74-75 0.6815845534806186 1106 76-77 0.459251076128222 1106 78-79 0.6624851965563288 1106 80-81 0.6756603088436144 1106 82-83 0.4954345432036824 1106 84-85 0.5314096357087301 1106 86-87 0.7891752393580767 1106 88-89 0.8394823897554744 1106 90-91 0.9941928313775321 1106 92-93 1.090172822895834 1106 94-95 1.027165798422807 1106 96-97 0.9902266353703659 1106 98-99 1.1483842878062376 1106 100-101 1.3281411190832237 1107 1 -0.03858982228729957 1107 2 -0.008562975793346084 1107 3 -0.02013159673010989 1107 4 -0.03226919965077002 1107 5 -0.013888411791484145 1107 6 0.014262645650013894 1107 7 0.024128780411096784 1107 8 -0.001885106396976255 1107 9 0.004702206240295936 1107 10-11 -0.00763510659918154 1107 12-13 -0.041599104851052005 1107 14-15 0.0014885416780572314 1107 16-17 0.005761118575613011 1107 18-19 -0.034121225306748215 1107 20-21 0.012412191414817642 1107 22-23 0.007874432518939045 1107 24-25 0.06325622092635541 1107 26-27 -0.05395421463807537 1107 28-29 -0.03564313923995144 1107 30-31 0.006778978141873893 1107 32-33 0.107455669070184 1107 34-35 0.08621760712689053 1107 36-37 0.1662017223527812 1107 38-39 0.12820744359961367 1107 40-41 0.10449214547296037 1107 42-43 0.16219355743660913 1107 44-45 0.12146013835604208 1107 46-47 0.07101132136233446 1107 48-49 0.12782144699927755 1107 50-51 0.15389591760353483 1107 52-53 0.16718083851582577 1107 54-55 0.04569654086596131 1107 56-57 0.21174029630496705 1107 58-59 0.11872560589439729 1107 60-61 0.1809208607255144 1107 62-63 0.3254185776374001 1107 64-65 0.34949019907172385 1107 66-67 0.28165407697583333 1107 68-69 0.2616749874516202 1107 70-71 0.2995236738848064 1107 72-73 0.33667261635580203 1107 74-75 0.2581050556231155 1107 76-77 0.12258048309180936 1107 78-79 0.4683854172986912 1107 80-81 0.454654307990328 1107 82-83 0.1796210890494585 1107 84-85 0.41645700720514256 1107 86-87 0.5044974721948954 1107 88-89 0.7720177734576374 1107 90-91 0.7370430511924049 1107 92-93 0.8416188940527825 1107 94-95 1.0115948826202512 1107 96-97 1.0723078973117879 1107 98-99 1.13223334362252 1107 100-101 1.0502195289962266 1108 1 0.08195986629991836 1108 2 0.05038931216630971 1108 3 0.10458934044697799 1108 4 0.04555342304207244 1108 5 0.03554813015207259 1108 6 0.12140250648594275 1108 7 0.1338910022962594 1108 8 0.14099126699500886 1108 9 -0.03380704134471557 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-0.6594808168140887 2316 62-63 -0.6505977317754557 2316 64-65 -0.6369238270233311 2316 66-67 -0.6747107878890333 2316 68-69 -0.5058795325856593 2316 70-71 -0.4952761863202291 2316 72-73 -0.3561640565239692 2316 74-75 -0.6966524001084764 2316 76-77 -0.528469843096449 2316 78-79 -0.4644841073984196 2316 80-81 -0.6478652633051034 2316 82-83 -0.5892572893289234 2316 84-85 -0.8364896377156406 2316 86-87 -0.8298019220269133 2316 88-89 -1.103035348629966 2316 90-91 -1.0983087661421713 2316 92-93 -1.002346856180207 2316 94-95 -0.8100001779575692 2316 96-97 -0.6592519815325772 2316 98-99 -0.8184560785582811 2316 100-101 -0.14341607212213248 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 8.0 4 33.0 5 78.0 6 182.0 7 389.0 8 984.0 9 1741.0 10 2971.0 11 4587.0 12 6529.0 13 8999.0 14 11242.0 15 14392.0 16 19585.0 17 27352.0 18 27377.0 19 20136.0 20 19326.0 21 20988.0 22 22508.0 23 25068.0 24 28765.0 25 33714.0 26 40128.0 27 48392.0 28 58275.0 29 70032.0 30 84417.0 31 101921.0 32 125497.0 33 160211.0 34 215472.0 35 308300.0 36 468798.0 37 648088.0 38 373390.0 39 16645.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.685510133105694 19.441984454639396 15.144529254465006 22.727976157789904 2 15.702322078207951 20.898935926872074 39.51189482456949 23.88684717035049 3 19.822497565076603 24.351886046247966 29.980155954440246 25.84546043423519 4 13.546627539928435 17.089780880099664 36.33568725837239 33.02790432159951 5 14.08695179415234 37.26105760209937 33.79514553515605 14.85684506859224 6 32.102136168740024 35.898064061856665 16.96258140998143 15.037218359421878 7 28.540816211517768 32.80486600130879 20.414304951198766 18.240012835974674 8 25.987279389972954 35.18833941870804 19.33664001124474 19.487741180074273 9 26.08941688474342 17.510662942289166 20.468307540936216 35.9316126320312 10-11 25.29230683548802 26.46446801979233 26.641675412942455 21.60154973177719 12-13 26.460373900803642 23.78217364236031 27.380340670480773 22.37711178635528 14-15 23.107172106997567 25.139760553502676 26.181133522857706 25.571933816642044 16-17 22.328870483195853 28.380869218135974 26.683365059284764 22.60689523938341 18-19 22.24384947849545 27.18380911731677 28.781548228293435 21.79079317589435 20-21 23.146292319432778 25.97993649640215 28.69425450900076 22.17951667516431 22-23 22.80928168267959 25.981693486737374 28.708774542337167 22.50025028824587 24-25 22.962174096453943 26.12540785856538 28.71988974040429 22.192528304576385 26-27 22.698479026255907 26.05017070158459 29.043933550614508 22.207416721545 28-29 22.29244474265396 26.65410694833321 28.872506151123712 22.18094215788912 30-31 22.57052990165495 26.168232819120828 28.64154479894395 22.61969248028027 32-33 22.44682783682665 26.371198353600604 28.71436044557275 22.467613363999998 34-35 23.027412840733387 26.213898833931033 28.48239623932355 22.276292086012027 36-37 22.842300268681452 26.043399531606298 28.779404338228932 22.33489586148332 38-39 22.661933353446877 26.388945066822274 28.797149402727264 22.15197217700359 40-41 22.79246732976302 26.30263225072389 28.587576259285218 22.317324160227876 42-43 22.500389126084976 26.499809622445753 28.126149455248346 22.873651796220926 44-45 22.778489148352673 26.318479086948273 28.099745481277417 22.803286283421638 46-47 23.011086373063318 26.676315831348692 27.754885138253126 22.557712657334868 48-49 23.08538059000111 26.651626837641125 27.62991743564837 22.633075136709394 50-51 22.68514320939483 26.87593060787642 27.8267646761928 22.612161506535948 52-53 23.06061417751967 27.06706597863367 27.214371385323894 22.65794845852276 54-55 22.706580624971405 26.700534589172563 27.401706335972353 23.191178449883672 56-57 22.880091708265347 26.951933385534733 27.361941998102452 22.80603290809747 58-59 23.28856131326839 26.595319184837944 27.3391768518827 22.776942650010966 60-61 23.01276417716093 26.614119925516057 27.884769346084138 22.488346551238873 62-63 22.826420631226636 26.617632628272197 27.853445543740445 22.702501196760725 64-65 22.62050231758814 26.9439429431082 27.597996086853033 22.837558652450625 66-67 22.735834888973535 26.826314967662245 27.302057423263093 23.135792720101126 68-69 22.412693960930337 26.85006719801834 27.65576381305491 23.08147502799641 70-71 22.818866034934608 26.72672812045265 27.35281349726829 23.10159234734445 72-73 22.741703856295427 26.57207539013473 27.4942798361822 23.19194091738764 74-75 22.488962454111057 27.2443562486864 27.074375721443438 23.192305575759104 76-77 22.715976015255418 26.682530628794897 27.22238978760341 23.379103568346267 78-79 22.603368136783544 26.790589691638967 27.353890735543562 23.252151436033927 80-81 22.705813594225923 26.921554535099435 27.223923227988845 23.1487086426858 82-83 22.635389365633667 26.736453438761593 27.41993925454547 23.208217941059274 84-85 22.49742003774552 26.777206957560658 27.21592485638238 23.509448148311446 86-87 22.479518103471445 26.748953715277167 27.363269591231415 23.408258590019972 88-89 22.4909596618037 26.68175429092467 27.044499890039255 23.78278615723238 90-91 22.47044988657196 26.541478985818706 27.253613714334403 23.734457413274935 92-93 22.557733707892734 26.764896791735648 27.147075340408588 23.53029415996303 94-95 22.47915251183453 26.527300516941793 27.182615028955006 23.810931942268674 96-97 22.15046003311098 26.47726421355455 27.379014640039088 23.99326111329538 98-99 22.212087297888097 26.575158410911637 26.94714310056416 24.2656111906361 100-101 22.496580489929766 25.649640611646422 27.80384907877106 24.049929819652753 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1468.0 1 1003.0 2 600.0 3 670.0 4 578.5 5 597.0 6 1048.0 7 2171.5 8 3486.5 9 4178.0 10 3813.0 11 3192.0 12 3209.0 13 3498.0 14 3723.5 15 3890.5 16 4082.0 17 4307.5 18 4508.0 19 4767.0 20 5385.0 21 6262.0 22 7413.5 23 8853.5 24 10435.5 25 12657.5 26 15720.5 27 19258.0 28 22315.0 29 25656.0 30 30327.5 31 35721.0 32 40144.5 33 44243.0 34 49847.5 35 54665.5 36 59844.5 37 65844.0 38 70723.0 39 74629.0 40 77904.5 41 82502.0 42 87070.5 43 92018.0 44 98451.5 45 103072.5 46 106471.0 47 117926.0 48 141428.5 49 142462.0 50 124305.5 51 116378.0 52 111205.5 53 109396.5 54 107933.0 55 101909.0 56 93706.0 57 84956.5 58 77123.0 59 68801.0 60 58678.5 61 47700.5 62 37932.5 63 30435.5 64 23517.5 65 17367.0 66 12732.0 67 9625.0 68 6752.0 69 4271.0 70 2894.5 71 1935.0 72 1249.0 73 870.0 74 632.5 75 397.5 76 233.5 77 117.5 78 68.5 79 34.5 80 19.5 81 11.5 82 5.0 83 4.0 84 2.5 85 3.5 86 2.5 87 0.5 88 1.5 89 2.0 90 1.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39008500518146394 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 7.790428844874991E-4 16-17 0.0021216487066893592 18-19 3.149322298992018E-4 20-21 0.0 22-23 0.0 24-25 1.491784246890956E-4 26-27 1.657538052101062E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0120171508777327 36-37 0.02264196979170051 38-39 0.010343037445110628 40-41 0.005569327855059569 42-43 0.004889737253698133 44-45 0.0014752088663699454 46-47 8.619197870925523E-4 48-49 0.002884116210655848 50-51 4.1438451302526556E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 5.801383182353717E-4 60-61 0.0010939751143867011 62-63 0.0015249350079329772 64-65 7.956182650085098E-4 66-67 0.002618910122319678 68-69 0.0021216487066893592 70-71 1.657538052101062E-5 72-73 0.0 74-75 0.0 76-77 0.0919270603695249 78-79 0.003530556050975262 80-81 1.9890456625212745E-4 82-83 0.0 84-85 1.657538052101062E-4 86-87 0.09159555275910469 88-89 0.43684415363123497 90-91 0.38963746990739667 92-93 0.0 94-95 0.0010442489728236693 96-97 0.0 98-99 0.0 100-101 2.652060883361699E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3016522.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.39997192143445 #Duplication Level Percentage of deduplicated Percentage of total 1 71.67878278022624 35.40929856705776 2 14.688912072312865 14.5126368785735 3 5.500980558218088 8.152448553489911 4 2.6209209563636997 5.178936866106635 5 1.469465479139477 3.62957767045037 6 0.9015448419577277 2.672177392717548 7 0.5998288064366782 2.0742068336947606 8 0.4532382744876301 1.7911966426726662 9 0.3113018778755558 1.3840473623528047 >10 1.6283380343355445 14.317459032077936 >50 0.09809274875187618 3.3079792988901686 >100 0.044636255181471636 3.9172977297777787 >500 0.0022134133142743566 0.789320714801873 >1k 0.0014756088760134543 1.5885485276857445 >5k 6.707313072788429E-5 0.20857920826359969 >10k+ 2.0121939218365286E-4 1.0662887213869583 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11746 0.3893888391995815 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10359 0.3434087336342981 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10076 0.33402706825940603 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6295 0.2086840407595237 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4902 0.16250503062798813 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3651 0.12103342856441955 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3281 0.10876764697887169 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3120 0.10343037445110627 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.3150761042021245E-5 0.0 0.0 0.0 0.0 3 3.3150761042021245E-5 0.0 0.0 0.0 0.0 4 3.3150761042021245E-5 0.0 0.0 0.0 0.0 5 3.3150761042021245E-5 0.0 0.0 0.0 0.0 6 6.630152208404249E-5 0.0 0.0 0.0 0.0 7 6.630152208404249E-5 0.0 0.0 0.0 0.0 8 9.945228312606373E-5 0.0 0.0 0.0 0.0 9 9.945228312606373E-5 0.0 0.0 0.0 0.0 10-11 1.3260304416808498E-4 0.0 0.0 6.630152208404249E-5 0.0 12-13 1.3260304416808498E-4 0.0 0.0 9.945228312606373E-5 3.3150761042021245E-5 14-15 2.9835684937819115E-4 0.0 0.0 1.1602766364707435E-4 3.3150761042021245E-5 16-17 3.315076104202124E-4 0.0 0.0 1.491784246890956E-4 3.3150761042021245E-5 18-19 3.978091325042549E-4 0.0 0.0 2.1547994677313805E-4 3.3150761042021245E-5 20-21 4.6411065458829736E-4 0.0 0.0 4.143845130252655E-4 3.3150761042021245E-5 22-23 4.972614156303186E-4 0.0 0.0 6.795906013614355E-4 3.3150761042021245E-5 24-25 5.635629377143611E-4 0.0 0.0 8.950705481345735E-4 3.3150761042021245E-5 26-27 5.635629377143611E-4 0.0 0.0 0.0011934273975127648 3.3150761042021245E-5 28-29 8.287690260505311E-4 0.0 0.0 0.0029006915911768587 3.3150761042021245E-5 30-31 8.619197870925523E-4 0.0 0.0 0.007790428844874991 3.3150761042021245E-5 32-33 8.78495167613563E-4 0.0 0.0 0.01737099878601913 3.3150761042021245E-5 34-35 9.61372070218616E-4 0.0 0.0 0.03127774304314704 3.3150761042021245E-5 36-37 0.0010608243533446798 0.0 0.0 0.05559382626746962 3.3150761042021245E-5 38-39 0.0011934273975127646 0.0 0.0 0.0900540423706507 3.3150761042021245E-5 40-41 0.001226578158554786 0.0 0.0 0.1282437190910592 3.3150761042021245E-5 42-43 0.001309455061159839 0.0 0.0 0.17034518561442613 3.3150761042021245E-5 44-45 0.001524935007932977 0.0 0.0 0.22320407409592902 3.3150761042021245E-5 46-47 0.0017238395741851044 0.0 0.0 0.28721819366807205 3.3150761042021245E-5 48-49 0.001790141096269147 0.0 0.0 0.3541164294508709 3.3150761042021245E-5 50-51 0.001790141096269147 0.0 0.0 0.4275453651589479 3.3150761042021245E-5 52-53 0.001790141096269147 0.0 0.0 0.5029633465295462 9.945228312606373E-5 54-55 0.001790141096269147 0.0 0.0 0.5911609462818438 1.3260304416808498E-4 56-57 0.001922744140437232 0.0 0.0 0.699166125756749 1.657538052101062E-4 58-59 0.001922744140437232 0.0 0.0 0.8207465418783619 1.657538052101062E-4 60-61 0.001922744140437232 0.0 0.0 0.9583553509637921 2.1547994677313805E-4 62-63 0.001922744140437232 0.0 0.0 1.0949696372179616 2.9835684937819115E-4 64-65 0.001922744140437232 0.0 0.0 1.23927490003388 2.9835684937819115E-4 66-67 0.0019890456625212744 0.0 0.0 1.4098521409756004 2.9835684937819115E-4 68-69 0.002055347184605317 0.0 0.0 1.6110441097396273 2.9835684937819115E-4 70-71 0.0021216487066893592 0.0 0.0 1.8310822861560432 2.9835684937819115E-4 72-73 0.0021713748482523913 0.0 0.0 2.061480075398091 3.6465837146223366E-4 74-75 0.002187950228773402 0.0 0.0 2.30649735025967 3.978091325042549E-4 76-77 0.002187950228773402 0.0 0.0 2.5678247995539234 3.978091325042549E-4 78-79 0.002187950228773402 0.0 0.0 2.874734545280956 4.309598935462761E-4 80-81 0.002486307078151593 0.0 0.0 3.211529702087371 4.6411065458829736E-4 82-83 0.0025194578391936145 0.0 0.0 3.551871990325282 4.8068603510930796E-4 84-85 0.0026023347417986676 0.0 0.0 3.9148065222133304 4.972614156303186E-4 86-87 0.002718362405445742 0.0 0.0 4.295327532834172 4.972614156303186E-4 88-89 0.002751513166487763 0.0 0.0 4.7132757526714535 4.972614156303186E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11825 0.0 21.310808 1 GAGTACT 10775 0.0 20.965834 12-13 TACATGG 12095 0.0 20.519552 2 GTACTTT 10975 0.0 20.389175 14-15 AGTACTT 10945 0.0 19.751274 12-13 ACATGGG 12100 0.0 19.452185 3 GTATAGA 775 0.0 19.054844 1 CATGGGG 6725 0.0 17.993681 4 GTATCAA 28175 0.0 17.127378 1 TACTTTT 12555 0.0 17.029448 14-15 AGAGTAC 20485 0.0 16.935589 10-11 ACTTTTT 13270 0.0 16.84422 16-17 TTTGCGT 570 0.0 16.6505 3 ACCGTGG 915 0.0 16.595907 8 GGTATCA 23325 0.0 16.420252 1 GTAAGCG 320 0.0 15.571333 94-95 TATCAAC 31795 0.0 15.148838 2 ATCAACG 31735 0.0 15.102713 3 TCAACGC 31755 0.0 15.093201 4 CAACGCA 31795 0.0 15.089138 5 >>END_MODULE