FastQCFastQC Report
Fri 27 May 2016
SRR938964_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938964_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3026557
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115630.3820512879816901No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92440.30542956897887596No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT90700.2996804619903078No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59140.19540355592179495No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT54840.1811959926741839No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT40430.13358413537230587No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC35900.11861663269517143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG115800.023.4181921
TACATGG120550.021.9852732
GAGTACT97200.021.71527112-13
GTACTTT101150.020.61028114-15
ACATGGG124800.020.4402033
GTATAGG6350.020.1583941
AGTACTT100350.019.35942512-13
GTATCAA256000.019.1860561
GTATTAG7350.018.705811
CATGGGG68450.017.976744
GGTATCA206900.017.5981331
AGAGTAC183500.017.5756810-11
ACTTTTT124200.017.20555716-17
TCAACGC288250.016.9442144
CAACGCA288600.016.9072655
TATCAAC288850.016.8926332
ATCAACG289200.016.8721893
TACTTTT115150.016.74817714-15
AACGCAG291450.016.740556
CATGGGA65150.016.0542094
Produced by FastQC (version 0.11.5)