##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938964_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3026557 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.562513443493714 33.0 31.0 34.0 30.0 34.0 2 31.635915662582928 33.0 31.0 34.0 30.0 34.0 3 31.67837215687661 33.0 31.0 34.0 28.0 34.0 4 35.21797309616174 37.0 35.0 37.0 33.0 37.0 5 35.244252462451556 37.0 35.0 37.0 33.0 37.0 6 35.31654252670609 37.0 35.0 37.0 33.0 37.0 7 35.214653482488515 37.0 35.0 37.0 32.0 37.0 8 35.193455467714635 37.0 35.0 37.0 32.0 37.0 9 36.75914545802375 39.0 37.0 39.0 32.0 39.0 10-11 36.63171104988275 39.0 37.0 39.0 32.0 39.0 12-13 36.4894165218101 39.0 37.0 39.0 32.0 39.0 14-15 37.642757595511995 40.0 37.0 41.0 32.0 41.0 16-17 37.560539253019186 40.0 37.0 41.0 32.0 41.0 18-19 37.48881055271716 40.0 37.0 41.0 32.0 41.0 20-21 37.35579025936072 40.0 37.0 41.0 31.5 41.0 22-23 37.442466472628794 40.0 37.0 41.0 32.0 41.0 24-25 37.35915959950531 40.0 37.0 41.0 31.0 41.0 26-27 37.258355286221274 40.0 37.0 41.0 31.0 41.0 28-29 37.1190914296344 40.0 37.0 41.0 31.0 41.0 30-31 36.9296175489178 40.0 36.5 41.0 30.0 41.0 32-33 36.72672842441097 39.5 36.0 41.0 30.0 41.0 34-35 36.391359059155334 39.0 35.5 41.0 30.0 41.0 36-37 36.11226436508548 39.0 35.0 41.0 29.0 41.0 38-39 35.889453593637924 39.0 35.0 41.0 27.5 41.0 40-41 35.72080403574094 39.0 35.0 41.0 27.0 41.0 42-43 35.50133815421286 39.0 35.0 40.0 26.5 41.0 44-45 35.41600736414348 39.0 35.0 41.0 26.0 41.0 46-47 35.16727423273376 39.0 35.0 41.0 24.5 41.0 48-49 34.96871164164429 39.0 35.0 41.0 24.0 41.0 50-51 33.80170669179533 38.0 33.0 40.0 22.5 40.5 52-53 33.94329678905767 38.0 33.5 39.5 23.0 40.5 54-55 34.427755201702794 38.0 34.0 40.0 23.0 41.0 56-57 34.29739981768061 38.0 34.0 40.0 22.5 41.0 58-59 34.06733112906844 38.0 34.0 40.0 22.0 41.0 60-61 33.77236295235808 37.5 33.5 40.0 21.5 41.0 62-63 33.39688827932201 37.0 33.0 40.0 19.5 41.0 64-65 32.96707479819478 36.5 32.5 39.5 18.0 41.0 66-67 32.562629416858826 36.0 32.0 39.0 15.5 41.0 68-69 32.16202585974756 35.5 31.5 39.0 13.0 40.5 70-71 31.72426109932838 35.0 31.0 38.5 9.5 40.0 72-73 31.277347163790406 35.0 31.0 37.5 7.5 39.5 74-75 30.8052701469029 35.0 30.5 37.0 6.5 39.0 76-77 30.3839997065973 35.0 30.0 36.0 2.0 39.0 78-79 29.95567141144211 34.0 30.0 36.0 2.0 38.5 80-81 29.507770876279547 34.0 29.0 35.5 2.0 37.0 82-83 29.115817907939615 34.0 29.0 35.0 2.0 37.0 84-85 28.75270183247829 34.0 29.0 35.0 2.0 36.0 86-87 28.3491568141621 34.0 28.5 35.0 2.0 36.0 88-89 27.91457966924132 34.0 27.0 35.0 2.0 36.0 90-91 27.45972453186905 34.0 26.5 35.0 2.0 35.0 92-93 27.002351021309032 33.0 25.5 35.0 2.0 35.0 94-95 26.48504059232983 33.0 25.0 35.0 2.0 35.0 96-97 25.849963671591183 33.0 23.5 35.0 2.0 35.0 98-99 25.078780112186884 32.5 18.0 35.0 2.0 35.0 100-101 22.40423193747879 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.44549661423672404 1101 2 0.36773322358062543 1101 3 0.29195217558877573 1101 4 0.25559761752242594 1101 5 0.1768067219436702 1101 6 0.2162195059811367 1101 7 0.28093168596156204 1101 8 0.3632126383782648 1101 9 0.298014937581236 1101 10-11 0.3312817661809504 1101 12-13 0.5979392892320092 1101 14-15 0.4097561133587817 1101 16-17 0.6038911434955807 1101 18-19 0.6269290885746628 1101 20-21 0.7996145180106851 1101 22-23 0.5530115435873952 1101 24-25 0.6242628229000999 1101 26-27 0.5798654897455151 1101 28-29 0.538486739775415 1101 30-31 0.6782524084062231 1101 32-33 0.6510216368395589 1101 34-35 0.6280305261802113 1101 36-37 0.6461615717326055 1101 38-39 0.6277536620954862 1101 40-41 0.6107553299455617 1101 42-43 0.6493543018130268 1101 44-45 0.8101007510061535 1101 46-47 0.7877062831807464 1101 48-49 0.7392581975467962 1101 50-51 0.7031040018896775 1101 52-53 0.7054919663205155 1101 54-55 0.7695010995293856 1101 56-57 0.8902074490598721 1101 58-59 0.7755780119599223 1101 60-61 0.728463555302433 1101 62-63 0.8109328043419808 1101 64-65 0.84628804070244 1101 66-67 0.7851476387496987 1101 68-69 0.7355086219945974 1101 70-71 0.6108161992817926 1101 72-73 0.5764640530690421 1101 74-75 0.5809770853114777 1101 76-77 0.6749097504191681 1101 78-79 0.615880036615426 1101 80-81 0.49732111791253075 1101 82-83 0.5801602544665556 1101 84-85 0.3943962207350218 1101 86-87 0.44142580528911424 1101 88-89 0.3858384320721413 1101 90-91 0.31956744209448473 1101 92-93 0.4421873901873852 1101 94-95 0.35419780321917216 1101 96-97 0.3199116012393901 1101 98-99 0.12639032184508991 1101 100-101 0.4081859263587013 1102 1 0.30970228332044414 1102 2 0.18948925265372552 1102 3 0.1825830345123194 1102 4 0.3599337560049065 1102 5 0.2798039225018414 1102 6 0.302699999778703 1102 7 0.39137779884669754 1102 8 0.29416658884441205 1102 9 0.4367431177728278 1102 10-11 0.252244192021422 1102 12-13 0.32567216940391575 1102 14-15 0.49409678916286737 1102 16-17 0.40568210138409455 1102 18-19 0.44744291884459386 1102 20-21 0.536243972932823 1102 22-23 0.5330708712076913 1102 24-25 0.47738287358112785 1102 26-27 0.5028795554425045 1102 28-29 0.4739424837677433 1102 30-31 0.48804917064200737 1102 32-33 0.5824781170765263 1102 34-35 0.35005622625468646 1102 36-37 0.3772563503883646 1102 38-39 0.48630437308521834 1102 40-41 0.4713897844824544 1102 42-43 0.3315718535427763 1102 44-45 0.5459802637149309 1102 46-47 0.4639564008942756 1102 48-49 0.5400179848919535 1102 50-51 0.44968260456583664 1102 52-53 0.5238424358904936 1102 54-55 0.44339994294043095 1102 56-57 0.4408345411503731 1102 58-59 0.366935698439562 1102 60-61 0.3244069329531314 1102 62-63 0.3824003282485293 1102 64-65 0.4678240524823494 1102 66-67 0.38621776177104294 1102 68-69 0.4104275066695777 1102 70-71 0.40121077504221603 1102 72-73 0.4302754032218523 1102 74-75 0.3783060582502067 1102 76-77 0.30466644724903347 1102 78-79 0.3907689578458111 1102 80-81 0.23713868333985388 1102 82-83 0.3039388160499925 1102 84-85 0.2530714940444412 1102 86-87 0.2965820860551496 1102 88-89 0.11864112706995655 1102 90-91 0.05066436099628291 1102 92-93 -0.10132552405723771 1102 94-95 -0.2329491631960856 1102 96-97 -0.27845239468558347 1102 98-99 -0.375394643354511 1102 100-101 -0.2116515861617394 1103 1 0.08830000300092422 1103 2 0.320431552353206 1103 3 0.3174990646626519 1103 4 0.3669218010363693 1103 5 0.34358172130718856 1103 6 0.32460139194982673 1103 7 0.3917997741414396 1103 8 0.28114009291623887 1103 9 0.41273268756883397 1103 10-11 0.4306160117853892 1103 12-13 0.518573150739023 1103 14-15 0.484163361365205 1103 16-17 0.510059241654055 1103 18-19 0.5222781951647519 1103 20-21 0.6773845279567041 1103 22-23 0.6975337360642087 1103 24-25 0.6220082881601812 1103 26-27 0.6469974138729668 1103 28-29 0.5546022289056864 1103 30-31 0.6482879705696618 1103 32-33 0.6355819651606254 1103 34-35 0.5270765991692201 1103 36-37 0.5272616205879004 1103 38-39 0.44911155684630444 1103 40-41 0.4786489453469116 1103 42-43 0.34700629166164987 1103 44-45 0.5071730895360318 1103 46-47 0.6331984384013296 1103 48-49 0.4736594053065488 1103 50-51 0.38572010922217714 1103 52-53 0.45922751379961113 1103 54-55 0.5144450474640578 1103 56-57 0.4715557320238304 1103 58-59 0.5321183647532877 1103 60-61 0.544460700966134 1103 62-63 0.4515670857462837 1103 64-65 0.3407960737116795 1103 66-67 0.3555903564938987 1103 68-69 0.2995877842174508 1103 70-71 0.2565534997114298 1103 72-73 0.3452982866677985 1103 74-75 0.21961987108386438 1103 76-77 0.12096555742375159 1103 78-79 0.22782936032136902 1103 80-81 0.20546963510195937 1103 82-83 0.2884738347292597 1103 84-85 0.27830137655492493 1103 86-87 0.2918638800310731 1103 88-89 0.26912550631830356 1103 90-91 0.23644919551800925 1103 92-93 0.3689103053943157 1103 94-95 0.3561996756232446 1103 96-97 0.26841656779179246 1103 98-99 0.23273727453099013 1103 100-101 0.552655149033491 1104 1 0.11581829352701334 1104 2 0.13339938386561911 1104 3 0.0526500887530581 1104 4 0.10653531150944673 1104 5 0.13769796983043392 1104 6 0.19790368049042684 1104 7 0.1290733636996535 1104 8 0.3125201482238893 1104 9 0.32616083119798134 1104 10-11 0.21068366352007217 1104 12-13 0.27119555665868944 1104 14-15 0.1939491352199667 1104 16-17 0.16102090017690074 1104 18-19 0.2651004554455838 1104 20-21 0.381290083991189 1104 22-23 0.26110486827264623 1104 24-25 0.3354395385611255 1104 26-27 0.24598218725234489 1104 28-29 0.38615833856808734 1104 30-31 0.4786817088355235 1104 32-33 0.39273889425187747 1104 34-35 0.3644698231576271 1104 36-37 0.29090114920761323 1104 38-39 0.2517705977716176 1104 40-41 0.3837812375006209 1104 42-43 0.30971696665999815 1104 44-45 0.3984597922799935 1104 46-47 0.44245409263707103 1104 48-49 0.3948788464409958 1104 50-51 0.3324101581016592 1104 52-53 0.24470569163289468 1104 54-55 0.24401585879751764 1104 56-57 0.39421519746223055 1104 58-59 0.39518129075602815 1104 60-61 0.2930650548815166 1104 62-63 0.3332408199235388 1104 64-65 0.4191361113083545 1104 66-67 0.501060558698498 1104 68-69 0.35172866422360016 1104 70-71 0.27338657037233816 1104 72-73 0.24914968763808432 1104 74-75 0.3312966966176383 1104 76-77 0.2493539100651212 1104 78-79 0.28382892205310384 1104 80-81 0.20707851601073557 1104 82-83 0.09481594176798325 1104 84-85 0.15594620874241016 1104 86-87 0.3028792833345193 1104 88-89 0.3949743694591987 1104 90-91 0.461486788049708 1104 92-93 0.4005457207430254 1104 94-95 0.42532102008230055 1104 96-97 0.49971525413721096 1104 98-99 0.4402236222254743 1104 100-101 0.7927326001430401 1105 1 0.015328246378899735 1105 2 0.09956174389011352 1105 3 0.14733506566348353 1105 4 0.1342382708346861 1105 5 0.11661707771899188 1105 6 0.2143891969766969 1105 7 0.1482373569048221 1105 8 0.20171109930851827 1105 9 0.24965787103622716 1105 10-11 0.2713042117608211 1105 12-13 0.34137068237711077 1105 14-15 0.31726752087018895 1105 16-17 0.3740447289068314 1105 18-19 0.36510392930929214 1105 20-21 0.3773733317075312 1105 22-23 0.2560530106908274 1105 24-25 0.42095636176271967 1105 26-27 0.31498624911686335 1105 28-29 0.4754217063499908 1105 30-31 0.34065284648871597 1105 32-33 0.3375059716903479 1105 34-35 0.28806759342509025 1105 36-37 0.3055553353702223 1105 38-39 0.2945304326108982 1105 40-41 0.335209710007895 1105 42-43 0.3455278541671092 1105 44-45 0.3138282234099492 1105 46-47 0.43205567997204497 1105 48-49 0.3994960644198997 1105 50-51 0.3714975729440724 1105 52-53 0.31874000109094425 1105 54-55 0.30792612111373074 1105 56-57 0.30205800826644236 1105 58-59 0.32999086899992847 1105 60-61 0.43879410210991665 1105 62-63 0.2741225144161632 1105 64-65 0.2546907810351158 1105 66-67 0.19011083573041532 1105 68-69 0.209679240321492 1105 70-71 0.12981236428671394 1105 72-73 0.19757472032319257 1105 74-75 0.3570376200449701 1105 76-77 0.2957089033840319 1105 78-79 0.19564465949220988 1105 80-81 0.21995772047503692 1105 82-83 0.10978922380586198 1105 84-85 0.23005138966739125 1105 86-87 0.2766729318801282 1105 88-89 0.244533008685206 1105 90-91 0.31987665105980767 1105 92-93 0.3472644938161906 1105 94-95 0.3904547562831304 1105 96-97 0.40963942050577273 1105 98-99 0.6202355797523182 1105 100-101 0.7173883303741739 1106 1 0.19997630289194746 1106 2 0.271908260734385 1106 3 0.23804164893888924 1106 4 0.1981113914856678 1106 5 0.24623606165226164 1106 6 0.25732645466787574 1106 7 0.1577819058968899 1106 8 0.1534104634830058 1106 9 0.3046611899056799 1106 10-11 0.19588542283912602 1106 12-13 0.25827118526869697 1106 14-15 0.2915028932106125 1106 16-17 0.32346858481503915 1106 18-19 0.2734839291122171 1106 20-21 0.3322515995174484 1106 22-23 0.28122793437027127 1106 24-25 0.38236459479841756 1106 26-27 0.3475882069363152 1106 28-29 0.22744348458322605 1106 30-31 0.23572702389280664 1106 32-33 0.33036933862742046 1106 34-35 0.41917458195192836 1106 36-37 0.25258381628650284 1106 38-39 0.3341864436612525 1106 40-41 0.4608506414770446 1106 42-43 0.522673630916934 1106 44-45 0.45201434704208765 1106 46-47 0.4204430520544804 1106 48-49 0.43588012255968067 1106 50-51 0.423187173103166 1106 52-53 0.2988045578046439 1106 54-55 0.15764476742705114 1106 56-57 0.3068054248333709 1106 58-59 0.40560829740910265 1106 60-61 0.36139227749312397 1106 62-63 0.27592734563306465 1106 64-65 0.0694473659229331 1106 66-67 0.279224216104204 1106 68-69 0.16127379809221765 1106 70-71 0.29945216617574033 1106 72-73 0.32203512410553614 1106 74-75 0.32037251054466864 1106 76-77 0.24416749903197044 1106 78-79 0.3388024249301047 1106 80-81 0.25803777241243253 1106 82-83 0.3395379616501124 1106 84-85 0.30777463178630526 1106 86-87 0.32323250491496225 1106 88-89 0.3442169434759137 1106 90-91 0.38884234398242157 1106 92-93 0.47838850218636964 1106 94-95 0.7262330871560501 1106 96-97 0.7821481409360338 1106 98-99 0.6939477374743106 1106 100-101 1.016136912800377 1107 1 0.08866271480342647 1107 2 0.04269435026243329 1107 3 0.11412329512040387 1107 4 0.12951038719196362 1107 5 0.05905073649640258 1107 6 0.11333911008370023 1107 7 0.06791352506501624 1107 8 0.10581281098178152 1107 9 0.12571739107720248 1107 10-11 0.24511804052507102 1107 12-13 0.3027496358733046 1107 14-15 0.2369133611642127 1107 16-17 0.19530550838137373 1107 18-19 0.2841523837865978 1107 20-21 0.21940706777316876 1107 22-23 0.22273962210263676 1107 24-25 0.39013644142992376 1107 26-27 0.36168581767342545 1107 28-29 0.40781914836609445 1107 30-31 0.4827296154895109 1107 32-33 0.4546321908677555 1107 34-35 0.3183680013489649 1107 36-37 0.17876701813692364 1107 38-39 0.32354125915271936 1107 40-41 0.23798087798634526 1107 42-43 0.17669380476228724 1107 44-45 0.4147383182230655 1107 46-47 0.5733354281483471 1107 48-49 0.49204652768226964 1107 50-51 0.47021145760387384 1107 52-53 0.4805604923078448 1107 54-55 0.5056857526869791 1107 56-57 0.4619886626720202 1107 58-59 0.5326986691808173 1107 60-61 0.39707888178612194 1107 62-63 0.38980092965180546 1107 64-65 0.522618573030293 1107 66-67 0.49771124656890464 1107 68-69 0.4314575291028717 1107 70-71 0.4956072238990714 1107 72-73 0.45243902309680095 1107 74-75 0.49399027380077953 1107 76-77 0.6091507200761086 1107 78-79 0.5747458563232115 1107 80-81 0.44339852773869026 1107 82-83 0.4872641943135605 1107 84-85 0.4927497910231047 1107 86-87 0.6263968920309999 1107 88-89 0.6152260156194274 1107 90-91 0.634536252552774 1107 92-93 0.5983212708451546 1107 94-95 0.5619747067862555 1107 96-97 0.4655789164690134 1107 98-99 0.44342068765594433 1107 100-101 0.6582782501229225 1108 1 0.2903108684675608 1108 2 0.2581503391768578 1108 3 0.21396914264156663 1108 4 0.30567749974151326 1108 5 0.2390049182257954 1108 6 0.30182850356557367 1108 7 0.3003690339557181 1108 8 0.32585666857603 1108 9 0.341609516487992 1108 10-11 0.36032755418272444 1108 12-13 0.460478582170488 1108 14-15 0.5255582066429625 1108 16-17 0.5776118633780172 1108 18-19 0.5156347930429064 1108 20-21 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-2.436561641427872 2316 68-69 -2.6062537138660105 2316 70-71 -2.4626207724532847 2316 72-73 -2.3795346855264583 2316 74-75 -2.4783301712233374 2316 76-77 -2.527873596613425 2316 78-79 -2.2480027402511524 2316 80-81 -2.2872693982179264 2316 82-83 -2.4387003057061136 2316 84-85 -2.7313499681459454 2316 86-87 -2.8408372499632364 2316 88-89 -3.0734360368097953 2316 90-91 -3.340955192899738 2316 92-93 -3.3990261402932305 2316 94-95 -3.6363877179729904 2316 96-97 -3.796683913105234 2316 98-99 -4.283352341809184 2316 100-101 -4.6983370437382455 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12500.0 3 3829.0 4 3877.0 5 4243.0 6 4919.0 7 5984.0 8 7336.0 9 8660.0 10 10780.0 11 12939.0 12 16111.0 13 21182.0 14 20113.0 15 19453.0 16 23013.0 17 29178.0 18 26070.0 19 20743.0 20 21070.0 21 22690.0 22 24704.0 23 27028.0 24 30581.0 25 35075.0 26 40085.0 27 46386.0 28 53696.0 29 63627.0 30 75409.0 31 90705.0 32 111119.0 33 141990.0 34 188296.0 35 266635.0 36 400036.0 37 581519.0 38 484110.0 39 70847.0 40 19.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.66008506786311 18.70293542774649 14.616073034971791 23.02090646941861 2 15.261334089055008 20.226941367527754 40.323328949574424 24.18839559384281 3 19.75978814212309 24.15448984329581 29.65778788682482 26.42793412775628 4 13.05921302865232 16.374520835193763 36.82419984851943 33.742066287634486 5 14.12476711138071 36.99966362829637 33.828177893995615 15.047391366327306 6 32.19124699545958 35.323309876782844 17.32873747632824 15.156705651429334 7 28.634795808020847 32.679645619686035 20.543111972406223 18.1424465998869 8 26.03324369405537 34.73339038853535 19.63255612155139 19.6008097958579 9 26.305788261563755 17.400137044106774 20.598681678023073 35.6953930163064 10-11 25.296802716780526 26.20676678613786 26.796098108461898 21.70033238861972 12-13 26.51904788281991 23.683811511156563 27.374270084005964 22.422870522017565 14-15 23.237658961884204 24.961722479334934 26.19154750984864 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22.77702586104523 26.283622622431263 28.52941308363903 22.40993843288447 42-43 22.501259697343695 26.442878851587963 28.061571248365468 22.99429020270287 44-45 22.755787820755145 26.28604899447908 28.08442969246555 22.873733492300214 46-47 22.967367658686875 26.599703821165537 27.765278689063898 22.667649831083693 48-49 23.072595812442884 26.59576890178208 27.621893064052117 22.70974222172292 50-51 22.63945892941446 26.793070677935148 27.92724707025619 22.640223322394203 52-53 22.951233207609377 27.010670721827896 27.35223086767145 22.685865202891282 54-55 22.656673320294153 26.633014698075247 27.491450617627557 23.21886136400305 56-57 22.854037435260402 26.850026728080895 27.43027027422114 22.865665562437563 58-59 23.18155618639155 26.551942367650405 27.415264865295846 22.851236580662203 60-61 22.91088336023102 26.553783276441727 27.978250977057474 22.55708238626978 62-63 22.758695168187995 26.536553433706718 27.943198545904043 22.76155285220125 64-65 22.556479076191284 26.793859889676124 27.750410099670425 22.899250934462167 66-67 22.67612278054429 26.72706890722443 27.39016709103488 23.206641221196403 68-69 22.348966591870074 26.72394404733745 27.770509041832845 23.156580318959634 70-71 22.769046954957243 26.602556537401433 27.49165323882259 23.136743268818734 72-73 22.700045207956602 26.42993431549835 27.645047734283583 23.224972742261464 74-75 22.428984201722013 27.09723366011307 27.273324086993156 23.20045805117176 76-77 22.670146323387065 26.548401543079862 27.30854202549588 23.472910108037194 78-79 22.606108724592044 26.633669074118654 27.473411452998864 23.28681074829044 80-81 22.70899285260525 26.780369782654347 27.2869038410934 23.223733523647006 82-83 22.6108658308659 26.592380895732475 27.53977203530507 23.256981238096554 84-85 22.519132613851454 26.614891975526607 27.35836249384568 23.507612916776267 86-87 22.51018869755307 26.58766151177027 27.54639348601246 23.355756304664197 88-89 22.530022521148993 26.485871564145558 27.255525558006212 23.72858035669924 90-91 22.528335276143256 26.331738866402816 27.411568909590034 23.72835694786389 92-93 22.645213372529707 26.478527619598164 27.386441017707348 23.489817990164784 94-95 22.548019589283236 26.298039326491658 27.374519139274884 23.779421944950226 96-97 22.24776741344385 26.189139454473054 27.640561708340066 23.922531423743035 98-99 22.330040285786755 26.24124202154271 27.250879828418917 24.177837864251625 100-101 22.51532707593705 25.547441859305792 27.781343963761717 24.15588710099544 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3811.0 1 2263.0 2 784.5 3 843.5 4 753.0 5 764.5 6 1228.5 7 2489.0 8 4048.5 9 4729.5 10 4256.0 11 3691.5 12 3830.0 13 4170.5 14 4456.5 15 4819.0 16 5164.0 17 5388.0 18 5561.0 19 5737.5 20 6120.5 21 6858.0 22 7890.0 23 9154.5 24 10582.0 25 12569.0 26 15306.5 27 18894.5 28 22288.5 29 25691.5 30 30375.0 31 35460.0 32 39493.5 33 43876.5 34 49598.0 35 54022.5 36 59007.5 37 65247.5 38 70468.0 39 74066.0 40 77611.0 41 82333.0 42 86576.0 43 91541.5 44 97918.0 45 102786.0 46 106513.5 47 117272.5 48 139651.5 49 140944.5 50 123260.0 51 115556.0 52 111183.5 53 109383.0 54 107692.0 55 102093.0 56 94182.0 57 85367.5 58 77343.0 59 69194.0 60 59225.5 61 48341.5 62 38552.5 63 31012.5 64 24045.0 65 17921.0 66 13169.0 67 9926.5 68 7116.5 69 4703.0 70 3264.5 71 2243.0 72 1516.0 73 1032.5 74 734.0 75 476.5 76 314.5 77 200.0 78 122.0 79 86.5 80 64.0 81 40.0 82 32.5 83 31.0 84 26.0 85 16.5 86 16.5 87 14.5 88 10.5 89 11.5 90 10.0 91 5.0 92 4.5 93 3.0 94 2.5 95 3.5 96 3.0 97 3.0 98 1.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2807150170969851 2 0.08313076542090567 3 0.08732695270566522 4 0.10133627088470497 5 0.1029552722780374 6 0.0787693739123367 7 0.08878074987518822 8 0.08541058370947582 9 0.08963981183899725 10-11 0.08380810273852433 12-13 0.08803733086804577 14-15 0.09633058290327921 16-17 0.0853279815975711 18-19 0.09566976600804147 20-21 0.09788350260708785 22-23 0.11133112642517554 24-25 0.12425009672707304 26-27 0.12697596641992867 28-29 0.11788973411040995 30-31 0.11019121728089046 32-33 0.1051855292994647 34-35 0.3809939809493097 36-37 0.5860785043863374 38-39 0.5697563270739656 40-41 0.5320402027782725 42-43 0.5585720011220671 44-45 0.5796851009249123 46-47 0.5691781122906325 48-49 0.5916128458839532 50-51 0.5826257361087203 52-53 0.5853681262239568 54-55 0.5756210770192003 56-57 0.5489405948739773 58-59 0.5432575695749329 60-61 0.4901774524649627 62-63 0.5658905482368249 64-65 0.5994270056701394 66-67 0.6131059154015602 68-69 0.6152700907334637 70-71 0.6113877914739422 72-73 0.6025658859225186 74-75 0.6000052204534724 76-77 0.5828239811772915 78-79 0.5598275532230188 80-81 0.5547227427073074 82-83 0.5678895193449189 84-85 0.5780000178420561 86-87 0.5688807446877756 88-89 0.5817666741449112 90-91 0.5956603493672843 92-93 0.60416836689347 94-95 0.6039701218248987 96-97 0.6001208634101389 98-99 0.6046474591425174 100-101 0.5810067347153878 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3026557.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.716904683507394 #Duplication Level Percentage of deduplicated Percentage of total 1 73.59179884575636 38.05940046393832 2 13.873040703187117 14.34941447434293 3 5.074606993476687 7.873288985636815 4 2.4817259446092095 5.1338873651176735 5 1.3213762914259024 3.416874585735995 6 0.821885418904573 2.5503221922151402 7 0.5450004451506646 1.9730015252028215 8 0.4025725030760454 1.665584301582786 9 0.2976530623647114 1.3854325549562876 >10 1.4625633695309603 13.53687518040106 >50 0.08621223157188368 3.0760553457489914 >100 0.03797943640949427 3.516070715770369 >500 0.0018563907422583226 0.6786355331283774 >1k 0.001408296424798878 1.4187154071513677 >5k 2.5605389541797784E-4 0.9836391510076408 >10k+ 6.401347385449446E-5 0.38280221806345416 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11563 0.3820512879816901 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9244 0.30542956897887596 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9070 0.2996804619903078 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5914 0.19540355592179495 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5484 0.1811959926741839 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4043 0.13358413537230587 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3590 0.11861663269517143 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.30408447618862E-5 2 6.60816895237724E-5 0.0 0.0 0.0 3.30408447618862E-5 3 6.60816895237724E-5 0.0 0.0 0.0 3.30408447618862E-5 4 9.91225342856586E-5 0.0 0.0 0.0 3.30408447618862E-5 5 1.321633790475448E-4 0.0 0.0 0.0 6.60816895237724E-5 6 1.321633790475448E-4 0.0 0.0 0.0 6.60816895237724E-5 7 1.982450685713172E-4 0.0 0.0 0.0 1.321633790475448E-4 8 1.982450685713172E-4 0.0 0.0 0.0 1.321633790475448E-4 9 1.982450685713172E-4 0.0 0.0 0.0 1.321633790475448E-4 10-11 1.982450685713172E-4 0.0 0.0 3.30408447618862E-5 1.321633790475448E-4 12-13 2.4780633571414647E-4 0.0 0.0 9.91225342856586E-5 1.486838014284879E-4 14-15 3.30408447618862E-4 0.0 0.0 1.1564295666660169E-4 1.65204223809431E-4 16-17 3.469288699998051E-4 0.0 0.0 1.486838014284879E-4 1.982450685713172E-4 18-19 4.956126714282929E-4 0.0 0.0 2.147654909522603E-4 2.3128591333320338E-4 20-21 5.616943609520653E-4 0.0 0.0 3.1388802523791887E-4 2.3128591333320338E-4 22-23 5.616943609520653E-4 0.0 0.0 5.616943609520654E-4 2.3128591333320338E-4 24-25 6.60816895237724E-4 0.0 0.0 9.416640757137566E-4 2.3128591333320338E-4 26-27 6.60816895237724E-4 0.0 0.0 0.0014042359023801634 2.3128591333320338E-4 28-29 8.755823861899843E-4 0.0 0.0 0.0032214823642839037 2.3128591333320338E-4 30-31 9.581844980946997E-4 0.0 0.0 0.009201875266185305 2.3128591333320338E-4 32-33 9.747049204756428E-4 0.0 0.0 0.01942801671998908 2.3128591333320338E-4 34-35 0.0011399091442850736 0.0 0.0 0.035056336292361254 2.3128591333320338E-4 36-37 0.001321633790475448 0.0 0.0 0.06046474591425174 2.3128591333320338E-4 38-39 0.0014537971695229926 0.0 0.0 0.09715660402232636 2.4780633571414647E-4 40-41 0.0014868380142848787 0.0 0.0 0.13812725152706523 2.643267580950896E-4 42-43 0.0016024809709514806 0.0 0.0 0.1804690940894224 2.643267580950896E-4 44-45 0.0017181239276180822 0.0 0.0 0.23326836401891654 2.643267580950896E-4 46-47 0.0018337668842846838 0.0 0.0 0.2985901141131656 2.8084718047603264E-4 48-49 0.0018833281514275132 0.0 0.0 0.3709660845640773 2.9736760285697575E-4 50-51 0.0018833281514275132 0.0 0.0 0.4506275612849849 2.9736760285697575E-4 52-53 0.0018833281514275132 0.0 0.0 0.5304542422297019 2.9736760285697575E-4 54-55 0.0018833281514275132 0.0 0.0 0.619862768155366 2.9736760285697575E-4 56-57 0.002015491530475058 0.0 0.0 0.7267664213824487 2.9736760285697575E-4 58-59 0.002015491530475058 0.0 0.0 0.8510991202214265 2.9736760285697575E-4 60-61 0.002032011952856001 0.0 0.0 0.9923982928456327 3.469288699998051E-4 62-63 0.002048532375236944 0.0 0.0 1.1368693865669801 3.964901371426344E-4 64-65 0.002048532375236944 0.0 0.0 1.2876843224826098 3.964901371426344E-4 66-67 0.002048532375236944 0.0 0.0 1.4637259433739396 3.964901371426344E-4 68-69 0.0020815732199988305 0.0 0.0 1.6677696802009676 3.964901371426344E-4 70-71 0.0021311344871416597 0.0 0.0 1.89553674356703 4.2953098190452054E-4 72-73 0.002213736599046375 0.0 0.0 2.1242619914311875 4.2953098190452054E-4 74-75 0.002246777443808261 0.0 0.0 2.3713249081381913 4.4605140428546366E-4 76-77 0.002246777443808261 0.0 0.0 2.6347595634247103 4.6257182666640677E-4 78-79 0.0022963387109510907 0.0 0.0 2.9396439584650147 4.956126714282929E-4 80-81 0.0024450225123795787 0.0 0.0 3.2792873221948238 5.121330938092361E-4 82-83 0.0024450225123795787 0.0 0.0 3.6330391266379585 5.616943609520653E-4 84-85 0.002494583779522408 0.0 0.0 3.995728479589183 5.616943609520653E-4 86-87 0.00256066546904618 0.0 0.0 4.380340433039919 5.616943609520653E-4 88-89 0.002577185891427123 0.0 0.0 4.800537376299207 5.616943609520653E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11580 0.0 23.418192 1 TACATGG 12055 0.0 21.985273 2 GAGTACT 9720 0.0 21.715271 12-13 GTACTTT 10115 0.0 20.610281 14-15 ACATGGG 12480 0.0 20.440203 3 GTATAGG 635 0.0 20.158394 1 AGTACTT 10035 0.0 19.359425 12-13 GTATCAA 25600 0.0 19.186056 1 GTATTAG 735 0.0 18.70581 1 CATGGGG 6845 0.0 17.97674 4 GGTATCA 20690 0.0 17.598133 1 AGAGTAC 18350 0.0 17.57568 10-11 ACTTTTT 12420 0.0 17.205557 16-17 TCAACGC 28825 0.0 16.944214 4 CAACGCA 28860 0.0 16.907265 5 TATCAAC 28885 0.0 16.892633 2 ATCAACG 28920 0.0 16.872189 3 TACTTTT 11515 0.0 16.748177 14-15 AACGCAG 29145 0.0 16.74055 6 CATGGGA 6515 0.0 16.054209 4 >>END_MODULE