FastQCFastQC Report
Fri 27 May 2016
SRR938964_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938964_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3026557
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116540.38505800485502173No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT105150.3474244826712334No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99880.3300119574817193No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64200.2121222233713094No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT49660.16408083508752683No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT36960.12211896223993139No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC32410.10708537787327316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG116150.021.8995041
TACATGG120100.020.7809622
GAGTACT111550.020.65141312-13
AGTACTT114350.020.2909712-13
ACATGGG120250.019.84753
GTACTTT116000.019.79782514-15
TTTGCGT6250.018.979423
AATTCGC3700.017.95350578-79
CATGGGG64050.017.6311554
TACTTTT132950.017.29161514-15
GTATCAA277050.017.1759051
AGAGTAC205600.017.14730310-11
TAGGACG1700.001801485316.7465464
ACTTTTT138550.016.69517716-17
GGTATCA229350.015.8466711
CTTTGCG7500.015.8161822
CCATGCG3654.2542524E-815.599789
ATTCGCG4350.015.30645280-81
TCGCGGA4400.015.2081982-83
CATGGGA64800.015.1571744
Produced by FastQC (version 0.11.5)