##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938964_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3026557 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.139150856897786 33.0 31.0 34.0 30.0 34.0 2 32.369443232029 34.0 31.0 34.0 30.0 34.0 3 32.46823601868394 34.0 31.0 34.0 30.0 34.0 4 36.04368924821175 37.0 35.0 37.0 35.0 37.0 5 35.95833912924819 37.0 35.0 37.0 35.0 37.0 6 35.99938477947053 37.0 35.0 37.0 35.0 37.0 7 35.93952963714214 37.0 35.0 37.0 35.0 37.0 8 35.904069541726784 37.0 35.0 37.0 35.0 37.0 9 37.579306452843944 39.0 37.0 39.0 35.0 39.0 10-11 37.49701442926731 39.0 37.0 39.0 34.5 39.0 12-13 37.41888984744051 39.0 37.0 39.0 34.0 39.0 14-15 38.66059849525385 40.0 38.0 41.0 34.0 41.0 16-17 38.65660369191791 40.0 38.0 41.0 34.0 41.0 18-19 38.75668540192701 40.0 38.0 41.0 34.0 41.0 20-21 38.75326104877588 40.0 38.0 41.0 34.0 41.0 22-23 38.69363669674816 40.0 38.0 41.0 34.0 41.0 24-25 38.628100676775624 40.0 38.0 41.0 34.0 41.0 26-27 38.46873856993277 40.0 38.0 41.0 34.0 41.0 28-29 38.28451636628684 40.0 38.0 41.0 34.0 41.0 30-31 38.11841326629566 40.0 37.5 41.0 33.0 41.0 32-33 37.9603465257717 40.0 37.0 41.0 33.0 41.0 34-35 37.932418421328265 40.0 37.0 41.0 33.0 41.0 36-37 37.8308472300373 40.0 37.0 41.0 33.0 41.0 38-39 37.70203865316265 40.0 37.0 41.0 33.0 41.0 40-41 37.54127462327655 40.0 37.0 41.0 32.0 41.0 42-43 37.365289667433984 40.0 37.0 41.0 32.0 41.0 44-45 37.106461401519944 40.0 36.0 41.0 31.0 41.0 46-47 36.785412268792555 40.0 36.0 41.0 30.5 41.0 48-49 36.485103369934876 39.0 36.0 41.0 30.0 41.0 50-51 36.20418465602994 39.0 35.0 41.0 30.0 41.0 52-53 35.964695526963474 39.0 35.0 40.5 29.0 41.0 54-55 35.70615504680731 39.0 35.0 40.0 28.0 41.0 56-57 35.40842961160156 38.0 35.0 40.0 28.0 41.0 58-59 35.08719445891817 38.0 34.0 40.0 27.5 41.0 60-61 34.65593857971285 37.5 34.0 40.0 26.0 41.0 62-63 34.33097575892342 37.0 34.0 40.0 26.0 41.0 64-65 34.0523996409121 36.5 33.5 39.5 26.0 41.0 66-67 33.58399329667341 36.0 33.0 39.0 25.5 40.5 68-69 33.1539059069431 35.5 32.5 39.0 24.0 40.0 70-71 32.67825155779323 35.0 32.0 38.0 23.5 40.0 72-73 32.152056776066004 35.0 31.5 37.0 22.0 39.5 74-75 31.682627982886164 35.0 31.0 37.0 20.5 39.0 76-77 29.72732299441246 33.0 28.5 35.0 18.0 37.0 78-79 30.712000633062587 34.0 30.5 36.0 18.5 37.5 80-81 30.71206473230142 34.0 31.0 35.0 18.0 37.0 82-83 30.39250788932771 34.0 30.5 35.0 17.5 37.0 84-85 29.98918325344608 34.0 30.0 35.0 12.5 36.0 86-87 29.39977770119644 34.0 29.5 35.0 7.0 36.0 88-89 28.821135699740665 34.0 29.0 35.0 2.0 35.0 90-91 28.34396444540777 33.5 28.5 35.0 2.0 35.0 92-93 27.966573238171293 33.0 27.0 35.0 2.0 35.0 94-95 27.543669093296444 33.0 27.0 35.0 2.0 35.0 96-97 26.881307373361878 33.0 25.5 34.5 2.0 35.0 98-99 25.93990729399777 32.5 24.5 34.0 2.0 35.0 100-101 21.555864469098054 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11297953937794603 1101 2 0.1328875507049574 1101 3 0.18563348356585863 1101 4 0.12054610080830486 1101 5 0.17106142247295963 1101 6 0.1704388252292901 1101 7 0.16474834066213617 1101 8 0.14144774986722553 1101 9 0.25750929208712137 1101 10-11 0.26605632851576644 1101 12-13 0.23753073241412892 1101 14-15 0.3251942219195527 1101 16-17 0.28814845230758834 1101 18-19 0.28632898768998416 1101 20-21 0.3196962795084275 1101 22-23 0.3110229744514399 1101 24-25 0.27521894488878473 1101 26-27 0.3198025734800538 1101 28-29 0.3272991351799206 1101 30-31 0.381787383022548 1101 32-33 0.40195999824100426 1101 34-35 0.4298007841250353 1101 36-37 0.4210181580927639 1101 38-39 0.4497373341955324 1101 40-41 0.48551502242920463 1101 42-43 0.4352248431819845 1101 44-45 0.4835830242695849 1101 46-47 0.5373888479250297 1101 48-49 0.3542554381734391 1101 50-51 0.32931668118878576 1101 52-53 0.3506861941228365 1101 54-55 0.39803639998699225 1101 56-57 0.256668761110312 1101 58-59 0.37882308793918895 1101 60-61 0.45474223569976857 1101 62-63 0.31135506104547517 1101 64-65 0.3208491988473625 1101 66-67 0.3300506752201784 1101 68-69 0.38072985475860577 1101 70-71 0.32910868014162276 1101 72-73 0.3516836763143303 1101 74-75 0.23319023609357714 1101 76-77 0.168780248077244 1101 78-79 0.4820161479284799 1101 80-81 0.6250826325574188 1101 82-83 0.48295052895207746 1101 84-85 0.281500368174342 1101 86-87 0.5114925733772715 1101 88-89 0.5115645553593922 1101 90-91 0.47176807812424215 1101 92-93 0.348945041985953 1101 94-95 0.41613822396621813 1101 96-97 0.423198724901944 1101 98-99 0.43331444399980157 1101 100-101 0.9358222359497894 1102 1 0.09258898728008091 1102 2 0.11101423614871919 1102 3 0.04938456439010963 1102 4 0.09035236573652128 1102 5 0.037975786948301504 1102 6 0.15343414237094777 1102 7 0.1398178986585279 1102 8 0.14101884456027136 1102 9 0.1589389764436291 1102 10-11 0.1881766274653387 1102 12-13 0.11292346756295757 1102 14-15 0.24411057254321378 1102 16-17 0.22324591911237235 1102 18-19 0.1107757340879516 1102 20-21 0.15766595650249116 1102 22-23 0.19483072899090104 1102 24-25 0.2027412248500866 1102 26-27 0.14205556768425964 1102 28-29 0.16062319411373238 1102 30-31 0.18802919658143225 1102 32-33 0.0700138297094739 1102 34-35 0.21667636734207463 1102 36-37 0.2345136935395189 1102 38-39 0.2722488701217003 1102 40-41 0.20304064117433285 1102 42-43 0.12935457633611236 1102 44-45 0.18354792423448174 1102 46-47 0.13898803976811536 1102 48-49 0.03237741507589931 1102 50-51 0.15920949766697134 1102 52-53 0.17800044216880195 1102 54-55 0.17766896254638453 1102 56-57 0.056118717877339463 1102 58-59 0.016100609363057572 1102 60-61 -0.0030110196144619295 1102 62-63 0.10116449353783707 1102 64-65 0.06844570254142468 1102 66-67 -0.11897422868278085 1102 68-69 -0.012213754038661762 1102 70-71 -0.09398157831448373 1102 72-73 0.09267409657304171 1102 74-75 0.03273564783410876 1102 76-77 -0.029734421169131053 1102 78-79 -0.05422677855834834 1102 80-81 0.12747970812522524 1102 82-83 0.08554664740185203 1102 84-85 0.16940027314741712 1102 86-87 0.19080837952234475 1102 88-89 0.1839964741327762 1102 90-91 0.20507715533576132 1102 92-93 0.2077127739244169 1102 94-95 0.09969342947264437 1102 96-97 -0.20556660996095033 1102 98-99 -0.08860381572333509 1102 100-101 0.48308058076911564 1103 1 0.040523727836543344 1103 2 0.07039537099036153 1103 3 0.1158162679940844 1103 4 0.09357952451206586 1103 5 0.053342251470873236 1103 6 0.048793252569623746 1103 7 0.063927863767411 1103 8 0.0288602546740222 1103 9 0.12511543470185416 1103 10-11 0.16509896269075597 1103 12-13 0.0739518392030547 1103 14-15 0.12784110724089004 1103 16-17 -0.02189453769415195 1103 18-19 0.06535188427905325 1103 20-21 0.1389498010651451 1103 22-23 0.10550635659381413 1103 24-25 -0.023078021679062033 1103 26-27 -0.015692104744097435 1103 28-29 0.09623303405538053 1103 30-31 0.08437443297983549 1103 32-33 -0.034233591785515216 1103 34-35 -0.09747130364894474 1103 36-37 -0.14549248696721406 1103 38-39 0.04560543625132141 1103 40-41 -0.022368368231774127 1103 42-43 -0.0735633175484196 1103 44-45 -0.05921591213540722 1103 46-47 0.09282240937439923 1103 48-49 -0.01677528057126665 1103 50-51 0.05172240067254563 1103 52-53 0.04753036432555291 1103 54-55 0.04533341373296196 1103 56-57 -0.137014511569177 1103 58-59 -0.10162037669127955 1103 60-61 -0.12997204289556663 1103 62-63 -0.030094557131540967 1103 64-65 -0.097358368208603 1103 66-67 -0.06436711606939127 1103 68-69 0.07026660548476826 1103 70-71 -0.13248248203392876 1103 72-73 -0.0328200744664926 1103 74-75 -0.011964867088344988 1103 76-77 -0.10245886279748007 1103 78-79 0.10887152383851628 1103 80-81 0.16655943546426144 1103 82-83 0.173051064188396 1103 84-85 0.19234133195445935 1103 86-87 0.2726391270642168 1103 88-89 0.45730399147352685 1103 90-91 0.45433160176717635 1103 92-93 0.3673248393275905 1103 94-95 0.18368567748823494 1103 96-97 0.03793475490095233 1103 98-99 0.3413285819898739 1103 100-101 0.9012560616448759 1104 1 -0.07989557726398289 1104 2 -0.06188931223334038 1104 3 0.002668587596474481 1104 4 -0.013536275426531574 1104 5 0.027550709455518074 1104 6 0.0062250242441876935 1104 7 -0.04776366557184275 1104 8 -0.0027160490050945896 1104 9 0.015490857243570133 1104 10-11 -0.008459302502103583 1104 12-13 0.03914306990090921 1104 14-15 0.04561499615197562 1104 16-17 -0.028603030363179016 1104 18-19 0.019621389143821943 1104 20-21 0.026896605542830798 1104 22-23 0.03881766188738567 1104 24-25 0.06136765256215426 1104 26-27 0.10738621756593147 1104 28-29 0.037782264945555255 1104 30-31 0.06171314142364537 1104 32-33 0.0804735220460131 1104 34-35 0.02157538935254877 1104 36-37 0.04736072838152694 1104 38-39 0.19128048381043072 1104 40-41 0.2096058631119675 1104 42-43 0.2110772176890663 1104 44-45 0.2526407148519425 1104 46-47 0.2407509929912095 1104 48-49 0.11215231042008611 1104 50-51 0.012247070397208404 1104 52-53 -0.04470310933820798 1104 54-55 -0.08811198078022642 1104 56-57 0.04434708690523337 1104 58-59 0.05962967681752929 1104 60-61 0.1702091103594654 1104 62-63 0.1557045668299537 1104 64-65 0.08928492019788337 1104 66-67 0.29530842074698427 1104 68-69 0.2869798282731537 1104 70-71 0.19194394208002308 1104 72-73 0.27843387436497835 1104 74-75 0.31788720195645936 1104 76-77 0.06698233686229216 1104 78-79 0.3409106772265993 1104 80-81 0.46090418586775783 1104 82-83 0.5596443477983577 1104 84-85 0.4472001392552798 1104 86-87 0.2123880904162867 1104 88-89 0.4034399203289283 1104 90-91 0.5071473152971997 1104 92-93 0.49821111950315 1104 94-95 0.572713923142814 1104 96-97 0.573384740558815 1104 98-99 0.44700109669990695 1104 100-101 1.107647865174524 1105 1 -3.7442207301552344E-4 1105 2 0.013797115683232164 1105 3 0.07138742085229666 1105 4 -0.06567536799988716 1105 5 0.027615771785811205 1105 6 0.011889164827152854 1105 7 5.268739819328516E-4 1105 8 0.04106578724208276 1105 9 -0.007316394092555356 1105 10-11 -0.05303089087458801 1105 12-13 -0.045629254349762505 1105 14-15 0.049444669477502146 1105 16-17 0.07634982791212508 1105 18-19 0.04444795446507044 1105 20-21 0.052406615788989086 1105 22-23 -0.009015989070171315 1105 24-25 -0.03919390505791398 1105 26-27 -0.019337890618309928 1105 28-29 0.025230231056447394 1105 30-31 0.00511222527632782 1105 32-33 -0.09375381340409206 1105 34-35 0.05604901360667469 1105 36-37 0.046326702060788705 1105 38-39 0.09953882215801002 1105 40-41 0.03978731381836553 1105 42-43 -0.03551598508662579 1105 44-45 -0.051447023559383354 1105 46-47 0.07696865948193476 1105 48-49 -0.13064072944418115 1105 50-51 -0.13634297662311212 1105 52-53 0.016479182414990134 1105 54-55 -0.010359010597710494 1105 56-57 -0.05351961223063029 1105 58-59 -0.024922306880633016 1105 60-61 -0.1492886409818297 1105 62-63 -0.04602246625458406 1105 64-65 -0.09364444383759007 1105 66-67 0.043852389695523186 1105 68-69 -0.022697287132665167 1105 70-71 0.00794418652849771 1105 72-73 -0.06564297307699007 1105 74-75 0.01752422386306307 1105 76-77 -0.1818661470285612 1105 78-79 -0.09173104740847648 1105 80-81 -0.09525686245368448 1105 82-83 -0.03523575801157719 1105 84-85 -0.2645512561864791 1105 86-87 -0.16471131704015818 1105 88-89 0.1135558114429358 1105 90-91 0.1511397792204292 1105 92-93 0.1301621726473421 1105 94-95 0.09821673005954423 1105 96-97 -0.012458323756113998 1105 98-99 0.17761429405916473 1105 100-101 0.6722297798682817 1106 1 -0.005637808627696472 1106 2 -0.10234943054265955 1106 3 -0.12096905707923611 1106 4 -0.12968039243680352 1106 5 -0.06543439912924498 1106 6 -0.05589012184433528 1106 7 -0.032163206315900084 1106 8 -0.05014803215409103 1106 9 -0.10037115588641399 1106 10-11 0.048290308056927245 1106 12-13 -0.003303759251473082 1106 14-15 -0.050999447658433894 1106 16-17 -0.08185650230507946 1106 18-19 -0.13010363984636086 1106 20-21 -0.12805887020815732 1106 22-23 -0.006155143757261783 1106 24-25 0.04492420728021784 1106 26-27 -0.08818550837771255 1106 28-29 -0.05955389462523897 1106 30-31 -0.02627007041389362 1106 32-33 -0.009268710693817184 1106 34-35 -0.03191345584718874 1106 36-37 -0.16580716975649779 1106 38-39 0.10948893610138555 1106 40-41 0.09652005032252475 1106 42-43 0.10233035752818864 1106 44-45 -0.008905533212320904 1106 46-47 0.10427016779836151 1106 48-49 0.02074641018302259 1106 50-51 0.09016851983317764 1106 52-53 0.26141331562123327 1106 54-55 0.24873971927408434 1106 56-57 0.2215639417634776 1106 58-59 0.3645888451477717 1106 60-61 0.3154305114478859 1106 62-63 0.21308914047237693 1106 64-65 0.240796205729211 1106 66-67 0.3028290693749014 1106 68-69 0.2021511914725025 1106 70-71 0.23368413541521704 1106 72-73 0.41012558482272965 1106 74-75 0.33735781682365484 1106 76-77 0.17829932391945746 1106 78-79 0.20395233961201242 1106 80-81 0.35799102753363243 1106 82-83 0.3578030924682025 1106 84-85 0.19710421246834642 1106 86-87 0.21391598108658627 1106 88-89 0.44909396097390797 1106 90-91 0.5810549196251813 1106 92-93 0.6761515249437657 1106 94-95 0.8008068366933401 1106 96-97 0.6650065190701113 1106 98-99 0.6725580569136262 1106 100-101 0.9162775375487122 1107 1 -0.1098261719008633 1107 2 -0.04415735470613669 1107 3 -0.06001832807326224 1107 4 -0.09832120593070215 1107 5 -0.09565029295242056 1107 6 0.013378775085378436 1107 7 -0.04456948141879735 1107 8 0.013669314704891633 1107 9 -0.05746596842773499 1107 10-11 -0.026373990643584477 1107 12-13 -8.061873461073787E-4 1107 14-15 0.056420247499069376 1107 16-17 0.07498569287018597 1107 18-19 0.03384861843714759 1107 20-21 -0.02373827241259363 1107 22-23 -0.04405046999720952 1107 24-25 -0.06099558962177554 1107 26-27 -0.037527745761394726 1107 28-29 4.995723295664334E-4 1107 30-31 -0.057243105456649346 1107 32-33 0.05343705533116605 1107 34-35 -0.07788475145893159 1107 36-37 0.021903316946513485 1107 38-39 -0.12950999580355926 1107 40-41 -0.08071908389845817 1107 42-43 -0.0452146566360625 1107 44-45 -0.04227900526768735 1107 46-47 -0.23069837138170612 1107 48-49 -0.1813236015820081 1107 50-51 -0.1651210832122274 1107 52-53 -0.15944168469865616 1107 54-55 -0.18059078639161896 1107 56-57 -0.07612079336317379 1107 58-59 -0.08063701652591959 1107 60-61 -0.15833534780881564 1107 62-63 -0.10470396142851257 1107 64-65 -0.23600277247169288 1107 66-67 -0.17852508544378765 1107 68-69 -0.15472962440284022 1107 70-71 -0.06459157306414198 1107 72-73 -0.05107321859090774 1107 74-75 -0.14665435741623867 1107 76-77 -0.5527309368485724 1107 78-79 -0.24502912689494138 1107 80-81 -0.10038531025098862 1107 82-83 -0.0207895656667354 1107 84-85 -0.10031477625356544 1107 86-87 -0.1474714161914612 1107 88-89 -0.0020757897209797704 1107 90-91 0.25815815385611884 1107 92-93 0.43836047570475856 1107 94-95 0.38239592577353676 1107 96-97 0.28488969284082444 1107 98-99 0.4133650640437381 1107 100-101 0.7888034579587462 1108 1 0.03329419664245137 1108 2 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-1.2148142501468584 2316 48-49 -1.2057579139068935 2316 50-51 -1.214535816959625 2316 52-53 -1.2581113411089504 2316 54-55 -1.324666096528297 2316 56-57 -1.2947465757840249 2316 58-59 -1.0805801999021938 2316 60-61 -1.1472728897850217 2316 62-63 -1.208481565296779 2316 64-65 -1.0472872051111608 2316 66-67 -1.2657491040822109 2316 68-69 -1.3673589372500317 2316 70-71 -1.3334420585825981 2316 72-73 -1.4208984462540677 2316 74-75 -1.6370816046696177 2316 76-77 -1.5770174067239786 2316 78-79 -1.6221255984037661 2316 80-81 -1.6154385514169682 2316 82-83 -1.8200508853587003 2316 84-85 -2.0282842748521333 2316 86-87 -2.1443212806248866 2316 88-89 -2.217564918485973 2316 90-91 -2.5963069128165195 2316 92-93 -2.7500088266632225 2316 94-95 -2.837806965059947 2316 96-97 -3.110811311029586 2316 98-99 -3.320432361901819 2316 100-101 -3.781563082176671 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 7.0 4 37.0 5 66.0 6 156.0 7 437.0 8 890.0 9 1716.0 10 2835.0 11 4436.0 12 6107.0 13 8438.0 14 10406.0 15 14049.0 16 18794.0 17 26096.0 18 25632.0 19 17923.0 20 16786.0 21 18014.0 22 19408.0 23 21537.0 24 24797.0 25 29490.0 26 35181.0 27 43056.0 28 52424.0 29 63666.0 30 76655.0 31 94049.0 32 115674.0 33 147919.0 34 198755.0 35 285128.0 36 446596.0 37 679548.0 38 486267.0 39 33581.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.60527986719719 19.46341453705123 15.191179387963219 22.740126207788364 2 15.70048077733213 20.913764386396817 39.53558449419588 23.85017034207517 3 19.823251305030766 24.42088485364723 29.918881422025095 25.836982419296913 4 13.530820665198112 17.079836923606592 36.38243059687956 33.006911814315735 5 14.071212483661252 37.282441910718084 33.80924406287295 14.83710154274771 6 32.049685500719136 35.934859313735046 16.981375206216175 15.034079979329649 7 28.529249573029684 32.86873500152153 20.38841495468283 18.213600470765957 8 26.00555680927205 35.20987709796974 19.305303022543438 19.47926307021477 9 26.099657135153908 17.54207834182538 20.425222455747573 35.93304206727314 10-11 25.250358740972 26.501417287036062 26.650728864515028 21.59749510747691 12-13 26.459042403628942 23.81518008747233 27.382087963319375 22.343689545579352 14-15 23.102537703418715 25.137963916265438 26.151241229188944 25.608257151126907 16-17 22.30359641630472 28.418908403643172 26.678440501109375 22.599054678942736 18-19 22.270206706828915 27.188369490480436 28.744626319610038 21.79679748308061 20-21 23.18854394614078 26.003772603654912 28.67737168009722 22.130311770107088 22-23 22.775483825350058 26.05280521728155 28.696386686257686 22.475324271110704 24-25 22.944768593487584 26.14008260872007 28.707736216433393 22.207412581358952 26-27 22.710905494262953 26.06289919535631 29.01597425721703 22.21022105316371 28-29 22.31268071277032 26.691798634554047 28.832911456813797 22.162609195861833 30-31 22.579782901825407 26.215399214354797 28.604880066689642 22.599937817130158 32-33 22.439045423562153 26.350024136337098 28.74571666748718 22.465213772613566 34-35 23.043844520950667 26.21044557230458 28.513022643313015 22.232687263431743 36-37 22.807106528537403 26.085236840227022 28.770043987068224 22.33761264416735 38-39 22.64753700197278 26.40253916641057 28.804338128141328 22.145585703475327 40-41 22.771101793718344 26.348179906118613 28.570647371193946 22.310070928969097 42-43 22.48904729516637 26.51297167999516 28.158367876405215 22.839613148433248 44-45 22.76211516988092 26.334619342541078 28.110405704984213 22.79285978259379 46-47 22.980991208793387 26.692085072526545 27.77546491100254 22.551458807677527 48-49 23.076718477102283 26.666817553739474 27.654015501499067 22.602448467659173 50-51 22.653996757354353 26.902196269602825 27.812162465335838 22.631644507706984 52-53 23.034623170817532 27.103421478597628 27.24729783711326 22.614657513471577 54-55 22.69443463314915 26.70916159847642 27.412584663034593 23.183819105339833 56-57 22.849759644374778 27.005273649232443 27.3426206742513 22.802346032141475 58-59 23.28191012113952 26.674847594696473 27.30368209495196 22.739560189212046 60-61 23.034819393868105 26.610249518999318 27.847750357425717 22.50718072970686 62-63 22.81521544117623 26.662788772853112 27.821977250056744 22.70001853591391 64-65 22.573918674795827 26.95403433444488 27.625937432111286 22.846109558648 66-67 22.74947654176911 26.847253285718487 27.27489148381286 23.12837868869955 68-69 22.401660658646428 26.892460292548183 27.637129895490187 23.068749153315203 70-71 22.76478235248541 26.759140296901773 27.36970216812407 23.106375182488744 72-73 22.754535929771023 26.56730073149126 27.497334429848834 23.180828908888877 74-75 22.51464287637735 27.254930272253258 27.06605228317193 23.16437456819746 76-77 22.733308786092632 26.679261187603444 27.196820900751405 23.39060912555252 78-79 22.61434204896326 26.8255987418038 27.342027486696924 23.21803172253602 80-81 22.691593120499633 26.94621313922057 27.204642106525668 23.15755163375413 82-83 22.551202571106376 26.799825676503037 27.446848019052673 23.202123733337913 84-85 22.53093201284496 26.853764855576816 27.18367769052425 23.431625441053978 86-87 22.49159891392665 26.75750257397266 27.367728082600077 23.383170429500613 88-89 22.479577867934392 26.724062689266663 27.03682869967062 23.759530743128323 90-91 22.477865984149375 26.646868581715587 27.207276500728543 23.66798893340649 92-93 22.588720450333497 26.785403347764476 27.165703470973785 23.460172730928246 94-95 22.472730564796898 26.577460791255543 27.215496024029946 23.734312619917617 96-97 22.140926602655178 26.526594725329915 27.399026611245848 23.933452060769064 98-99 22.197847256800387 26.634770136495035 26.994304088771496 24.17307851793308 100-101 22.388447315625175 25.97715155728367 27.603804593477992 24.03059653361317 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1493.0 1 1010.0 2 588.0 3 679.5 4 611.5 5 618.0 6 1059.0 7 2234.5 8 3561.5 9 4196.5 10 3820.5 11 3203.0 12 3236.0 13 3517.5 14 3714.0 15 3843.5 16 3975.5 17 4173.5 18 4536.5 19 4971.0 20 5406.0 21 6210.0 22 7526.5 23 8983.0 24 10531.0 25 12719.5 26 15664.5 27 19204.0 28 22522.5 29 26057.0 30 30848.0 31 36131.0 32 40226.0 33 44209.0 34 49724.0 35 54730.5 36 60040.0 37 66174.5 38 71290.0 39 74678.5 40 77934.0 41 83330.0 42 88035.0 43 92651.0 44 99306.5 45 103897.0 46 107319.5 47 118714.5 48 142098.0 49 143121.0 50 124418.0 51 116526.0 52 111491.0 53 109498.0 54 107937.5 55 101837.0 56 93647.5 57 85005.5 58 77544.0 59 69188.5 60 58666.0 61 47529.5 62 37880.0 63 30400.5 64 23449.0 65 17242.5 66 12487.0 67 9368.0 68 6511.0 69 4161.0 70 2796.5 71 1876.0 72 1198.5 73 842.0 74 606.0 75 376.5 76 233.5 77 114.0 78 59.5 79 32.5 80 16.5 81 10.5 82 6.0 83 6.0 84 4.5 85 1.0 86 0.5 87 2.5 88 3.5 89 2.5 90 2.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4018757948388218 2 0.0 3 0.0 4 0.0 5 2.973676028569758E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001354674635237334 16-17 0.0023789408228558065 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.002593706313808066 36-37 0.007037699934281759 38-39 0.012125990027612233 40-41 0.002593706313808066 42-43 7.103781623805532E-4 44-45 3.4692886999980504E-4 46-47 3.30408447618862E-4 48-49 3.6344929238074815E-4 50-51 5.286535161901792E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.8172464619037407E-4 60-61 0.0017842056171418546 62-63 0.0 64-65 1.486838014284879E-4 66-67 0.00165204223809431 68-69 0.0015529197038086514 70-71 1.65204223809431E-5 72-73 0.0 74-75 0.0 76-77 0.12601778192183397 78-79 6.60816895237724E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14794038242134544 88-89 0.4388485001273724 90-91 0.339395557394095 92-93 0.0 94-95 0.0 96-97 5.616943609520653E-4 98-99 0.0 100-101 1.65204223809431E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3026557.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.37216361865879 #Duplication Level Percentage of deduplicated Percentage of total 1 71.65547090127131 35.377856335096105 2 14.708514403224962 14.523823594068444 3 5.465076803239489 8.094679983542303 4 2.6306365183787492 5.19520866426458 5 1.4736901713793615 3.6379636132275572 6 0.9110492848733511 2.6988284614457503 7 0.6194673996800597 2.1409112069400305 8 0.4390862275752407 1.7342909656435546 9 0.31613274915608025 1.4047342034895367 >10 1.6363140469115356 14.391909027970101 >50 0.09697693278193989 3.317823012073245 >100 0.04370540492617634 3.8487588472893948 >500 0.0020733423217359254 0.7506355450699044 >1k 0.0015382862386352475 1.6087217351491407 >5k 1.3376402075089108E-4 0.5418101365065705 >10k+ 1.3376402075089108E-4 0.7320446682236813 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11654 0.38505800485502173 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10515 0.3474244826712334 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9988 0.3300119574817193 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6420 0.2121222233713094 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4966 0.16408083508752683 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3696 0.12211896223993139 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3241 0.10708537787327316 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 3.30408447618862E-5 0.0 0.0 0.0 6 0.0 3.30408447618862E-5 0.0 0.0 3.30408447618862E-5 7 3.30408447618862E-5 3.30408447618862E-5 0.0 0.0 3.30408447618862E-5 8 3.30408447618862E-5 3.30408447618862E-5 0.0 0.0 3.30408447618862E-5 9 3.30408447618862E-5 3.30408447618862E-5 0.0 0.0 3.30408447618862E-5 10-11 4.95612671428293E-5 3.30408447618862E-5 0.0 3.30408447618862E-5 3.30408447618862E-5 12-13 9.91225342856586E-5 3.30408447618862E-5 0.0 9.91225342856586E-5 6.60816895237724E-5 14-15 1.65204223809431E-4 3.30408447618862E-5 0.0 1.1564295666660169E-4 9.91225342856586E-5 16-17 2.973676028569758E-4 3.30408447618862E-5 0.0 1.486838014284879E-4 9.91225342856586E-5 18-19 4.1301055952357743E-4 3.30408447618862E-5 0.0 2.147654909522603E-4 9.91225342856586E-5 20-21 4.956126714282929E-4 3.30408447618862E-5 0.0 2.973676028569758E-4 9.91225342856586E-5 22-23 4.956126714282929E-4 3.30408447618862E-5 0.0 5.12133093809236E-4 9.91225342856586E-5 24-25 6.938577399996101E-4 3.30408447618862E-5 0.0 8.755823861899843E-4 9.91225342856586E-5 26-27 6.938577399996101E-4 3.30408447618862E-5 0.0 0.001338154212856391 9.91225342856586E-5 28-29 7.599394295233825E-4 3.30408447618862E-5 0.0 0.003204961941902961 9.91225342856586E-5 30-31 7.599394295233825E-4 3.30408447618862E-5 0.0 0.009218395688566248 9.91225342856586E-5 32-33 7.764598519043257E-4 3.30408447618862E-5 0.0 0.019593220943798513 9.91225342856586E-5 34-35 9.086232309518704E-4 3.30408447618862E-5 0.0 0.035535428541408606 1.1564295666660169E-4 36-37 9.912253428565859E-4 3.30408447618862E-5 0.0 0.061720298015203415 1.321633790475448E-4 38-39 0.001024266187618472 3.30408447618862E-5 0.0 0.09903993217375387 1.321633790475448E-4 40-41 0.0010573070323803583 3.30408447618862E-5 0.0 0.1407870395303971 1.321633790475448E-4 42-43 0.00117294998904696 3.30408447618862E-5 0.0 0.18390534194465857 1.321633790475448E-4 44-45 0.001354674635237334 3.30408447618862E-5 0.0 0.23744803088129515 1.321633790475448E-4 46-47 0.0016685626604752528 3.30408447618862E-5 0.0 0.3034966795603057 1.65204223809431E-4 48-49 0.0017842056171418546 3.30408447618862E-5 0.0 0.37684735493169297 1.65204223809431E-4 50-51 0.0017842056171418546 3.30408447618862E-5 0.0 0.4572026893926002 1.65204223809431E-4 52-53 0.0017842056171418546 3.30408447618862E-5 0.0 0.53742586047446 2.147654909522603E-4 54-55 0.0017842056171418546 3.30408447618862E-5 0.0 0.6273134786491713 2.3128591333320338E-4 56-57 0.0018833281514275132 3.30408447618862E-5 0.0 0.7349275100386347 2.3128591333320338E-4 58-59 0.0018833281514275132 3.30408447618862E-5 0.0 0.8603505567547547 2.3128591333320338E-4 60-61 0.0018833281514275132 3.30408447618862E-5 0.0 1.0024261892308652 2.3128591333320338E-4 62-63 0.0018833281514275132 3.30408447618862E-5 0.0 1.1484832435007832 2.3128591333320338E-4 64-65 0.0018833281514275132 3.30408447618862E-5 0.0 1.3004711294054596 2.3128591333320338E-4 66-67 0.002048532375236944 3.30408447618862E-5 0.0 1.4769753221234558 2.4780633571414647E-4 68-69 0.002048532375236944 3.30408447618862E-5 0.0 1.6835136427300064 2.9736760285697575E-4 70-71 0.002048532375236944 3.30408447618862E-5 0.0 1.9147995560632096 3.1388802523791887E-4 72-73 0.002065052797617887 3.30408447618862E-5 0.0 2.1462011123530798 4.625718266664067E-4 74-75 0.0020980936423797736 3.30408447618862E-5 0.0 2.395593408615797 4.956126714282929E-4 76-77 0.0021146140647607167 3.30408447618862E-5 0.0 2.6610270350104095 4.956126714282929E-4 78-79 0.0021146140647607167 3.30408447618862E-5 0.0 2.967266104685952 4.956126714282929E-4 80-81 0.002411981667617692 3.30408447618862E-5 0.0 3.31039527753814 5.286535161901792E-4 82-83 0.0024450225123795787 3.30408447618862E-5 0.0 3.6663938594250824 6.112556280948947E-4 84-85 0.002527624624284294 3.30408447618862E-5 0.0 4.030966540527736 6.277760504758377E-4 86-87 0.002577185891427123 4.95612671428293E-5 0.0 4.418205241137041 6.442964728567808E-4 88-89 0.002577185891427123 6.60816895237724E-5 0.0 4.840764604796804 6.77337317618667E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11615 0.0 21.899504 1 TACATGG 12010 0.0 20.780962 2 GAGTACT 11155 0.0 20.651413 12-13 AGTACTT 11435 0.0 20.29097 12-13 ACATGGG 12025 0.0 19.8475 3 GTACTTT 11600 0.0 19.797825 14-15 TTTGCGT 625 0.0 18.97942 3 AATTCGC 370 0.0 17.953505 78-79 CATGGGG 6405 0.0 17.631155 4 TACTTTT 13295 0.0 17.291615 14-15 GTATCAA 27705 0.0 17.175905 1 AGAGTAC 20560 0.0 17.147303 10-11 TAGGACG 170 0.0018014853 16.746546 4 ACTTTTT 13855 0.0 16.695177 16-17 GGTATCA 22935 0.0 15.846671 1 CTTTGCG 750 0.0 15.816182 2 CCATGCG 365 4.2542524E-8 15.59978 9 ATTCGCG 435 0.0 15.306452 80-81 TCGCGGA 440 0.0 15.20819 82-83 CATGGGA 6480 0.0 15.157174 4 >>END_MODULE