##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938963_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 837294 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.02078361961271 33.0 31.0 34.0 30.0 34.0 2 32.22389865447501 34.0 31.0 34.0 30.0 34.0 3 32.3455393207165 34.0 31.0 34.0 30.0 34.0 4 35.82912214825378 37.0 35.0 37.0 35.0 37.0 5 35.771982123364076 37.0 35.0 37.0 35.0 37.0 6 35.8819148351714 37.0 35.0 37.0 35.0 37.0 7 35.840172030374035 37.0 35.0 37.0 35.0 37.0 8 35.85684598241478 37.0 35.0 37.0 35.0 37.0 9 37.43491414007505 39.0 37.0 39.0 34.0 39.0 10-11 37.32618829228443 39.0 37.0 39.0 33.5 39.0 12-13 37.299411556753064 39.0 37.0 39.0 34.0 39.0 14-15 38.315522385207586 40.0 37.5 41.0 33.5 41.0 16-17 38.5524851485858 40.0 38.0 41.0 34.0 41.0 18-19 38.687630629145794 40.0 38.0 41.0 34.0 41.0 20-21 38.63453876416169 40.0 38.0 41.0 34.0 41.0 22-23 38.588368601709796 40.0 38.0 41.0 34.0 41.0 24-25 38.424814939555276 40.0 38.0 41.0 34.0 41.0 26-27 38.14010610371029 40.0 37.5 41.0 33.5 41.0 28-29 38.04748152978524 40.0 37.0 41.0 33.0 41.0 30-31 38.02937916669653 40.0 37.0 41.0 33.0 41.0 32-33 37.9267121226236 40.0 37.0 41.0 33.0 41.0 34-35 37.73255809787243 40.0 37.0 41.0 33.0 41.0 36-37 37.544718462093364 40.0 36.5 41.0 32.5 41.0 38-39 37.35757392266038 39.5 36.0 41.0 32.0 41.0 40-41 37.39359532016233 40.0 37.0 41.0 32.0 41.0 42-43 37.230534913662346 40.0 36.0 41.0 31.5 41.0 44-45 37.04095574553263 40.0 36.0 41.0 31.0 41.0 46-47 36.7878887224798 39.0 36.0 41.0 31.0 41.0 48-49 36.448586756861985 39.0 35.5 41.0 30.0 41.0 50-51 36.22134280193099 39.0 35.0 40.0 30.0 41.0 52-53 36.06831292234269 39.0 35.0 40.0 28.5 41.0 54-55 35.90877517335608 39.0 35.0 40.0 28.0 41.0 56-57 35.744502528383094 38.5 35.0 40.0 28.0 41.0 58-59 35.50604506899607 38.0 35.0 40.0 28.0 41.0 60-61 34.943747954720806 38.0 34.0 40.0 26.5 41.0 62-63 34.60683224769316 38.0 34.0 40.0 26.0 41.0 64-65 34.15992829280994 37.0 33.5 39.5 26.0 40.5 66-67 33.56183431387302 36.5 32.5 39.0 25.0 40.0 68-69 32.98852493867148 36.0 32.0 38.5 23.5 40.0 70-71 32.252474041376146 35.0 31.5 37.5 22.0 39.0 72-73 31.61623456038142 35.0 31.0 37.0 21.0 39.0 74-75 31.140463803634088 34.0 30.5 36.5 20.0 38.5 76-77 29.209078889852307 32.5 28.5 34.5 18.0 36.0 78-79 30.177842549928698 34.0 29.5 35.0 18.0 37.0 80-81 30.103234347791815 34.0 29.5 35.0 17.5 36.5 82-83 29.665219146440798 34.0 29.5 35.0 10.5 36.0 84-85 29.190130348479748 34.0 29.0 35.0 7.0 36.0 86-87 28.671063569068927 33.0 29.0 35.0 2.0 35.0 88-89 27.887840471805603 33.0 26.5 35.0 2.0 35.0 90-91 27.188853616531347 32.0 25.5 34.0 2.0 35.0 92-93 26.853102972193756 32.0 25.0 34.0 2.0 35.0 94-95 26.293321700621284 32.0 25.0 34.0 2.0 35.0 96-97 25.264787517884997 31.5 21.5 34.0 2.0 35.0 98-99 24.12052039068714 31.0 10.0 34.0 2.0 35.0 100-101 18.55678351928952 24.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21620945707866923 1101 2 0.18680874405966819 1101 3 0.15513712069935792 1101 4 0.1848059443111154 1101 5 0.06784313294291877 1101 6 0.16160960023783844 1101 7 0.26614487793886354 1101 8 0.292893953855895 1101 9 0.35682577037245977 1101 10-11 0.35471573524145583 1101 12-13 0.24523551784162834 1101 14-15 0.48320740957002073 1101 16-17 0.32139686149564994 1101 18-19 0.43235128819988233 1101 20-21 0.3771062816617743 1101 22-23 0.5384939718035042 1101 24-25 0.49903675730506336 1101 26-27 0.37295541701602275 1101 28-29 0.5226990324444998 1101 30-31 0.5302456782618066 1101 32-33 0.6346022859213889 1101 34-35 0.5794808433320995 1101 36-37 0.7029320171038691 1101 38-39 0.7467838844737074 1101 40-41 0.7440492599916482 1101 42-43 0.7494800380316775 1101 44-45 0.689577425551299 1101 46-47 0.7692987010849919 1101 48-49 0.8184488758756814 1101 50-51 0.7118467925061438 1101 52-53 0.7660134368481124 1101 54-55 0.8393983402248608 1101 56-57 0.842846154490303 1101 58-59 0.5531972801547411 1101 60-61 0.7605714713802669 1101 62-63 0.8076248361225353 1101 64-65 0.8693512365765059 1101 66-67 0.7706257883322678 1101 68-69 0.9270490671704152 1101 70-71 0.9091449276151096 1101 72-73 1.0433365906023795 1101 74-75 1.2164749317958048 1101 76-77 0.9323420726075184 1101 78-79 1.105474502956234 1101 80-81 1.0863807435890855 1101 82-83 0.930322601958192 1101 84-85 1.0697324330587463 1101 86-87 1.3097748955527635 1101 88-89 1.288096124325449 1101 90-91 1.6130044063779856 1101 92-93 1.588242008975083 1101 94-95 1.3090296829536712 1101 96-97 1.3027418150198322 1101 98-99 2.0463210077499667 1101 100-101 2.6059892413154806 1102 1 0.1295639825021624 1102 2 0.0719363878079946 1102 3 0.08631896567430175 1102 4 0.055095616324926766 1102 5 0.11847258012409156 1102 6 0.1575010013016538 1102 7 0.09913787472495983 1102 8 0.02789107678405145 1102 9 0.3375037769125342 1102 10-11 0.20733246716853415 1102 12-13 0.1385680792776398 1102 14-15 0.2846315601321052 1102 16-17 0.2581732077547443 1102 18-19 0.22162682747542561 1102 20-21 0.3578334089406212 1102 22-23 0.3469529138831362 1102 24-25 0.3754700302900602 1102 26-27 0.2914904260682789 1102 28-29 0.1276033873488558 1102 30-31 0.3474656549645445 1102 32-33 0.3929668460100828 1102 34-35 0.35901819614530694 1102 36-37 0.4739872709177462 1102 38-39 0.47123232874974263 1102 40-41 0.4532351890051629 1102 42-43 0.7097378517377706 1102 44-45 0.6445740572720595 1102 46-47 0.7180903309111102 1102 48-49 0.9783053029734887 1102 50-51 0.7740424193569453 1102 52-53 0.6372942951979397 1102 54-55 0.7843304834328677 1102 56-57 0.7836082978386543 1102 58-59 0.8107811713593165 1102 60-61 0.7492614212771116 1102 62-63 0.7712474204347757 1102 64-65 0.6832503004755779 1102 66-67 0.45272338422296343 1102 68-69 0.4581442491276704 1102 70-71 0.529510035049185 1102 72-73 0.8138234472961301 1102 74-75 1.0062662388284913 1102 76-77 0.49189921406120973 1102 78-79 1.1176652177331547 1102 80-81 1.057825805551385 1102 82-83 1.1004768410779526 1102 84-85 1.2545948248176906 1102 86-87 1.349139904745357 1102 88-89 0.9844089111554766 1102 90-91 1.2038111092536532 1102 92-93 1.4604298466801602 1102 94-95 1.5154823937167308 1102 96-97 1.7083984189522958 1102 98-99 1.5588994255007975 1102 100-101 1.3441774579519752 1103 1 0.0013061598734793733 1103 2 0.1001574186780374 1103 3 0.08955664043515554 1103 4 0.0814542834120715 1103 5 0.08789337272369124 1103 6 0.10458569242608462 1103 7 0.08319302732316203 1103 8 0.10723688427555089 1103 9 0.11337714318325709 1103 10-11 0.05178742354402033 1103 12-13 0.045804667805619204 1103 14-15 0.013258176341501837 1103 16-17 0.035438528894239596 1103 18-19 0.13280977011532258 1103 20-21 0.14659615465701847 1103 22-23 0.17670255472548746 1103 24-25 0.13750268735582694 1103 26-27 0.32918883876669014 1103 28-29 0.18614783529727674 1103 30-31 0.0632379314984064 1103 32-33 0.2589887809061935 1103 34-35 0.3902955709657263 1103 36-37 0.23565901835107894 1103 38-39 0.10617661551522417 1103 40-41 0.42725757941828846 1103 42-43 0.4004435527083743 1103 44-45 0.47399958024063693 1103 46-47 0.44332947734231 1103 48-49 0.25444321950875093 1103 50-51 0.22576725204203996 1103 52-53 0.31050901890935023 1103 54-55 0.272092843711782 1103 56-57 0.4320621679812575 1103 58-59 0.4099743806518674 1103 60-61 0.40590233371273143 1103 62-63 0.5374252823079999 1103 64-65 0.43346229108492196 1103 66-67 0.33100047872522254 1103 68-69 0.31645831058742147 1103 70-71 0.556493286039057 1103 72-73 0.3648518645497134 1103 74-75 0.5132181292240894 1103 76-77 0.36712697256711735 1103 78-79 0.4665448210829588 1103 80-81 0.2508923110351091 1103 82-83 0.43537995611351477 1103 84-85 0.6950675113168678 1103 86-87 0.4049801584332968 1103 88-89 0.45856795667213746 1103 90-91 0.3323089543077913 1103 92-93 0.6477612177863676 1103 94-95 0.7458364705939857 1103 96-97 0.38530896241913126 1103 98-99 1.0108966923987275 1103 100-101 1.1275589709692504 1104 1 -0.007610918757251284 1104 2 0.0019338811857707583 1104 3 0.06569014222857561 1104 4 -0.0889758833803782 1104 5 0.02523639533860944 1104 6 -0.06557652107164103 1104 7 -0.03279683115340504 1104 8 -0.00495414268088723 1104 9 0.025583433302053038 1104 10-11 0.0510106981707068 1104 12-13 0.01681313886072644 1104 14-15 0.04221493919435915 1104 16-17 0.07970593949874427 1104 18-19 0.11845391796448013 1104 20-21 0.10572479767708387 1104 22-23 0.15293950684156243 1104 24-25 0.019687185070594637 1104 26-27 0.11600061853621924 1104 28-29 0.15061688923559302 1104 30-31 -0.06956599622203896 1104 32-33 -0.001609365467537316 1104 34-35 0.15497152554934246 1104 36-37 0.07635060919656667 1104 38-39 0.21121515500017551 1104 40-41 0.21357754617850588 1104 42-43 -0.006741156994756636 1104 44-45 0.19142841574614522 1104 46-47 0.09083183041317255 1104 48-49 -0.012223729809072381 1104 50-51 -0.12647770475472697 1104 52-53 -0.04891301600937226 1104 54-55 0.03527216043087833 1104 56-57 0.07929741571086879 1104 58-59 0.02618216316876243 1104 60-61 0.13710108552523081 1104 62-63 0.105297173498613 1104 64-65 0.18100788978246385 1104 66-67 -0.056854283805407135 1104 68-69 -0.1144266984432818 1104 70-71 0.041918661894428055 1104 72-73 0.11963474606518076 1104 74-75 0.13851088515764687 1104 76-77 0.1296406458167283 1104 78-79 0.1335390804380019 1104 80-81 0.006442590435778328 1104 82-83 0.2774342081761638 1104 84-85 0.5459879648023787 1104 86-87 0.38052942028980397 1104 88-89 0.37717205567918555 1104 90-91 0.2743657502308281 1104 92-93 0.45034069222480966 1104 94-95 0.6986665152811327 1104 96-97 0.6851122372154101 1104 98-99 0.7945608689087216 1104 100-101 1.2598931324704665 1105 1 0.10743301859037047 1105 2 -0.02448114696569803 1105 3 0.08645733567568925 1105 4 0.004862364036881672 1105 5 -0.04827315511479213 1105 6 0.04131138168737891 1105 7 -0.06922711898249645 1105 8 -0.0027265805459393277 1105 9 0.12222805821215843 1105 10-11 -0.0068445278303741475 1105 12-13 0.11392257004308703 1105 14-15 -0.06881237036662213 1105 16-17 0.014387503352601527 1105 18-19 -0.07080993413942593 1105 20-21 -0.08082914616028347 1105 22-23 -0.15200848747551987 1105 24-25 -0.16902416146440657 1105 26-27 -0.1733018212582138 1105 28-29 -0.008074988055547294 1105 30-31 -0.01763924267459771 1105 32-33 0.005310109038276778 1105 34-35 0.12193520050066553 1105 36-37 0.10982543072850603 1105 38-39 0.17484625667874099 1105 40-41 0.3147737645848352 1105 42-43 0.21385765380277633 1105 44-45 0.14499163816909544 1105 46-47 0.13015619503176623 1105 48-49 0.32543610215908103 1105 50-51 0.23237904721411695 1105 52-53 0.3626685531064382 1105 54-55 0.4471606770849803 1105 56-57 0.45498366270716417 1105 58-59 0.38939757018119536 1105 60-61 0.3166303157008059 1105 62-63 0.3767001868464419 1105 64-65 -0.15270547383636313 1105 66-67 0.03533521683480245 1105 68-69 0.24547367515572205 1105 70-71 0.3346150644692827 1105 72-73 0.21700727513749385 1105 74-75 0.48486171742397133 1105 76-77 0.38485436900266734 1105 78-79 0.5018585865155636 1105 80-81 0.6282306036959078 1105 82-83 0.4659350763992016 1105 84-85 0.214528473107503 1105 86-87 0.2159003006153455 1105 88-89 0.615749222290308 1105 90-91 0.5397438218986963 1105 92-93 0.8235011994775441 1105 94-95 1.0321518607286606 1105 96-97 0.6751846768344443 1105 98-99 1.1614859561695212 1105 100-101 0.9988561759868553 1106 1 0.032713217843173936 1106 2 0.04401200371923153 1106 3 0.08023068561342228 1106 4 0.06837419467199624 1106 5 0.057579895943739245 1106 6 0.013914121413407088 1106 7 0.11010040281077949 1106 8 0.18282759130960358 1106 9 0.10977475808762449 1106 10-11 0.2198055344361265 1106 12-13 0.19300102583387257 1106 14-15 -0.0656990453354851 1106 16-17 0.0679366938880932 1106 18-19 0.053100402099680366 1106 20-21 0.07421444039015057 1106 22-23 0.13130409035760238 1106 24-25 0.31603187838615554 1106 26-27 0.10378410651264147 1106 28-29 0.096532732990525 1106 30-31 0.2588240200900316 1106 32-33 0.3552478425376506 1106 34-35 0.05406471482196906 1106 36-37 0.11854274081567695 1106 38-39 0.15180765144835817 1106 40-41 0.27803652921746647 1106 42-43 0.3109933947741368 1106 44-45 0.1798608785177933 1106 46-47 0.26776516140785844 1106 48-49 0.23079102121263162 1106 50-51 0.10411005593143585 1106 52-53 0.05195871499933702 1106 54-55 0.19124535952582278 1106 56-57 0.1922190300795208 1106 58-59 0.19823941326961148 1106 60-61 0.4125207266597073 1106 62-63 0.5622543587019848 1106 64-65 0.17792964446999093 1106 66-67 0.18664281334787347 1106 68-69 0.1956604746575863 1106 70-71 0.2910285140334139 1106 72-73 0.22012060016862733 1106 74-75 0.16730256424838075 1106 76-77 0.09656047111972654 1106 78-79 0.30820976957907575 1106 80-81 -0.07724884835888446 1106 82-83 0.09669472770679377 1106 84-85 0.21639646812618452 1106 86-87 0.3155267016116099 1106 88-89 0.6082772422155891 1106 90-91 0.6817114433183704 1106 92-93 0.9169009504115415 1106 94-95 1.4300347997074923 1106 96-97 0.9286093343686908 1106 98-99 1.1459193310138573 1106 100-101 1.0125548061238412 1107 1 -0.04419339438021552 1107 2 -0.029413072916462113 1107 3 0.058941828922165485 1107 4 0.04973465783896813 1107 5 0.011062628964140231 1107 6 0.0148092023598565 1107 7 0.1338918938539848 1107 8 0.04247949762247316 1107 9 0.013448780982542985 1107 10-11 0.0014125190610840832 1107 12-13 0.013285086807137247 1107 14-15 0.09100747588301772 1107 16-17 0.05506728589882215 1107 18-19 0.04833686512295543 1107 20-21 0.10037385460606885 1107 22-23 -0.020275922095699173 1107 24-25 0.06605433024935792 1107 26-27 0.11765037722822314 1107 28-29 0.18015095552141958 1107 30-31 0.1455266795880661 1107 32-33 0.044002153295387814 1107 34-35 0.13762905288116656 1107 36-37 0.09571218951765559 1107 38-39 0.0876948592747695 1107 40-41 0.03568138863886361 1107 42-43 0.14700573431814945 1107 44-45 0.1855157544637649 1107 46-47 0.12407323689402006 1107 48-49 0.22855331884650099 1107 50-51 0.053524511339034575 1107 52-53 0.28866347000461445 1107 54-55 0.1373302036200812 1107 56-57 0.055503647858998306 1107 58-59 0.13685527087091742 1107 60-61 -0.05549285222716094 1107 62-63 0.15491843691828677 1107 64-65 0.31045700580727953 1107 66-67 0.030199727324308867 1107 68-69 0.006148039318958354 1107 70-71 0.056193999233151715 1107 72-73 0.4610463054564917 1107 74-75 0.2828293747666315 1107 76-77 0.12162423441123238 1107 78-79 0.5392858424787796 1107 80-81 0.4584795499551291 1107 82-83 0.21016345076455778 1107 84-85 0.4086540179393836 1107 86-87 0.6651089472769023 1107 88-89 0.742526661460726 1107 90-91 0.448755401072944 1107 92-93 0.5637061999565169 1107 94-95 0.8223130052348395 1107 96-97 0.7839669476458262 1107 98-99 0.8280061867950579 1107 100-101 0.7883707068327226 1108 1 -0.03453149339107142 1108 2 0.028478805024569454 1108 3 0.03263606929477447 1108 4 0.15430196553127473 1108 5 -0.15627327327344886 1108 6 -0.07620983634176781 1108 7 0.08131788780385563 1108 8 0.12680017850213687 1108 9 0.20105231742736862 1108 10-11 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68-69 -1.1731177546065297 2316 70-71 -1.022824239858398 2316 72-73 -1.0468075144470639 2316 74-75 -1.084170362730454 2316 76-77 -0.9830159907579379 2316 78-79 -1.0766274246130756 2316 80-81 -1.2013191005854331 2316 82-83 -1.2913143434331538 2316 84-85 -1.3235170513224972 2316 86-87 -1.310757190819075 2316 88-89 -1.2144672183323806 2316 90-91 -1.606804070421184 2316 92-93 -1.7320859755121027 2316 94-95 -1.5246970136264792 2316 96-97 -1.4611852554342057 2316 98-99 -1.4159228256106324 2316 100-101 -1.158409865034745 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 6.0 5 38.0 6 87.0 7 145.0 8 273.0 9 410.0 10 729.0 11 1177.0 12 1656.0 13 2333.0 14 2842.0 15 3622.0 16 4599.0 17 6420.0 18 6525.0 19 5104.0 20 5244.0 21 5620.0 22 6239.0 23 7131.0 24 8352.0 25 9523.0 26 11115.0 27 13656.0 28 16968.0 29 19999.0 30 24458.0 31 30191.0 32 36548.0 33 46474.0 34 62328.0 35 89258.0 36 131580.0 37 175515.0 38 96747.0 39 4378.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.39545991880894 19.80009159770862 14.942535625993608 17.861912857488832 2 13.648849746922826 26.955406344724793 34.78336163880309 24.612382269549286 3 28.090730376665785 25.146244927110427 30.74165108074344 16.021373615480343 4 11.53214999749192 16.69640532477242 42.781030319099386 28.990414358636272 5 10.341289917281147 42.35167097817493 35.07967332860381 12.227365775940111 6 25.483521917032725 39.52231832546274 15.871008271885383 19.12315148561915 7 23.615599777378076 35.438567576024674 22.653213805425573 18.292618841171677 8 22.24057499516299 27.314300592145653 20.76104689631121 29.684077516380146 9 19.86840942369108 24.641882062931302 26.596034367856454 28.893674145521164 10-11 24.537080165389934 24.122829018242097 27.766650662730175 23.57344015363779 12-13 31.96768399152508 23.33857641401945 25.37710768260611 19.316631911849363 14-15 23.84382397412593 27.09157922143688 31.36072978647678 17.70386701796041 16-17 18.19754560926816 29.56507718491535 31.346869308172344 20.89050789764414 18-19 17.536397186160112 34.954734918606455 28.67429444995163 18.8345734452818 20-21 21.88156131538026 27.694931529426942 25.441183144749637 24.982324010443165 22-23 18.871626931519874 28.032925113520463 27.96138512876003 25.13406282619964 24-25 24.94886792847183 28.127924231973893 26.627731646945364 20.295476192608916 26-27 23.552448187193733 23.615627982080348 33.9260569478068 18.905866882919124 28-29 18.277630079756932 32.93843022880852 29.28815923677944 19.495780454655115 30-31 20.122382341208702 28.241991462974774 27.779728506354996 23.85589768946153 32-33 21.025589577854372 28.820044094428006 29.33234921067152 20.822017117046105 34-35 24.677308768266727 25.950156358623854 28.284188069920614 21.08834680318881 36-37 22.410812735115943 24.96640134179601 33.16620614105822 19.456579782029827 38-39 21.49529919481317 27.889493153941068 31.571601427143193 19.04360622410257 40-41 20.139638297275138 29.586692856063625 30.033640515291715 20.240028331369526 42-43 18.560087811758915 30.703897482876886 26.15523164949827 24.580783055865933 44-45 21.530060714420078 26.199523337758375 27.10489495455824 25.165520993263307 46-47 24.42305912281697 29.36691021250295 27.1823428771054 19.027687787574685 48-49 22.649026001170473 27.61200090771197 28.753150118838604 20.985822972278953 50-51 17.46279806781056 29.25311884732682 33.40377898418526 19.880304100677364 52-53 20.438698951622726 31.832426841706734 26.75708890783882 20.971785298831712 54-55 17.614004161023487 26.966991283826232 29.763261172300293 25.655743382849987 56-57 22.7630318621655 26.588569845239547 27.335977565825146 23.31242072676981 58-59 26.158876132750102 25.956675574399473 26.784587134048937 21.099861158801485 60-61 20.9161837025359 27.82440708128428 32.01561011731474 19.243799098865072 62-63 20.175986002334934 27.71794466025505 30.640136393630584 21.46593294377943 64-65 19.117567143902185 32.39735455264769 28.14140901422749 20.34366928922264 66-67 20.919725011704678 27.129355812686917 26.32358421158238 25.627334964026026 68-69 18.444689817604402 28.87019593955858 28.5280735625814 24.157040680255616 70-71 23.68134729258779 28.338074798099594 25.688646998545313 22.291930910767306 72-73 21.532579953994656 24.551053751728784 31.421161503605667 22.4952047906709 74-75 18.55029416190729 32.834643506343056 26.981263451069754 21.633798880679905 76-77 20.97800113084444 26.374436221449464 27.31341963294176 25.334143014764333 78-79 20.77865151898795 27.118915329380794 29.593451559118467 22.508981592512793 80-81 23.04669281845092 27.324892226900666 27.685220738536103 21.94319421611231 82-83 20.67218921907956 26.220121008869047 30.946597013713223 22.16109275833817 84-85 19.732077382615902 28.07024772660499 29.172130697222244 23.02554419355686 86-87 20.148466474010895 28.979711374958534 27.980431592757178 22.89139055827339 88-89 19.97334967694579 28.107035469397108 26.07431865966386 25.845296193993246 90-91 21.099356778461697 24.553787910120413 28.265663502407513 26.081191809010377 92-93 22.625923510738165 28.718526586838077 28.023907970199236 20.63164193222452 94-95 21.126642811758018 27.360489391890923 28.243702256343127 23.26916554000793 96-97 16.55183245072818 27.86727242760607 31.214901814655306 24.36599330701044 98-99 20.36387457691086 29.274723096069007 25.748840908928045 24.612561418092092 100-101 19.76845495907325 26.00352208348735 28.08022563310018 26.147797324339223 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3538.0 1 2207.0 2 842.0 3 747.5 4 560.5 5 541.0 6 879.5 7 1520.0 8 2013.0 9 1994.0 10 1535.5 11 1079.5 12 956.0 13 907.5 14 863.0 15 812.5 16 743.0 17 754.5 18 817.5 19 880.0 20 987.5 21 1132.5 22 1319.5 23 1541.5 24 1808.0 25 2199.5 26 2903.0 27 3702.0 28 4219.5 29 5072.0 30 6537.0 31 7933.0 32 10018.5 33 12739.0 34 14992.0 35 16941.5 36 19922.5 37 22358.5 38 22315.5 39 21560.0 40 20415.5 41 19145.5 42 18127.5 43 18145.5 44 19102.0 45 20392.0 46 27837.5 47 64849.0 48 139919.5 49 137665.5 50 66496.0 51 30610.5 52 14303.0 53 8096.0 54 6055.0 55 4967.0 56 4110.5 57 3384.0 58 2780.5 59 2261.0 60 1751.0 61 1339.0 62 983.0 63 642.0 64 365.5 65 217.5 66 154.0 67 108.0 68 81.5 69 63.0 70 48.0 71 44.5 72 37.0 73 38.5 74 37.0 75 24.5 76 18.5 77 15.0 78 13.0 79 11.5 80 6.5 81 2.5 82 2.5 83 1.5 84 0.0 85 0.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38361674632805204 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.554588949640151E-4 16-17 0.0022692148755395357 18-19 4.7772944748200754E-4 20-21 0.0 22-23 0.0 24-25 1.791485428057528E-4 26-27 1.1943236187050188E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.013854153976978218 36-37 0.02472249890719389 38-39 0.011584939101438683 40-41 0.007106225531294861 42-43 0.005792469550719341 44-45 0.0016123368852517756 46-47 0.0013734721615107717 48-49 0.002866376684892045 50-51 6.568779902877603E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 7.763103521582623E-4 60-61 0.001970633970863281 62-63 0.0013734721615107717 64-65 7.763103521582623E-4 66-67 0.002627511961151041 68-69 0.002448363418345289 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.08969370376474692 78-79 0.003105241408633049 80-81 2.985809046762547E-4 82-83 0.0 84-85 0.0 86-87 0.08855909632697714 88-89 0.42195453448848314 90-91 0.3742413059212176 92-93 0.0 94-95 0.0011943236187050188 96-97 0.0 98-99 0.0 100-101 6.568779902877603E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 837294.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.681629987702216 #Duplication Level Percentage of deduplicated Percentage of total 1 75.74227887899137 20.209274594746002 2 12.356985653244118 6.594090379264086 3 4.210324349534703 3.370149492674939 4 2.041857066327689 2.179202989261221 5 1.1657537681929855 1.555210534984741 6 0.7765080303931222 1.2431099969657227 7 0.5204407104920753 0.9720344527520483 8 0.39406497006855634 0.8411436577987335 9 0.30204677853355744 0.7253190345428834 >10 2.0782276683083922 11.05848199978145 >50 0.24467079660424537 4.588368910588906 >100 0.13329414513170867 6.375453030252739 >500 0.00849861999161901 1.6018594266576611 >1k 0.01610264840517286 10.250850087504086 >5k 0.0044729578903257945 8.47200082850152 >10k+ 0.0044729578903257945 19.96345058372326 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 45503 5.4345307621934476 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 23336 2.787073596610032 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 17059 2.0373966611488914 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 13708 1.6371788165208396 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 11979 1.430680262846742 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 11838 1.4138402998230013 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 11518 1.3756219440244406 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 11380 1.3591402780863115 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 10638 1.270521465578399 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 10315 1.231944812694227 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 9890 1.1811860588992635 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 9067 1.0828932250798406 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 8110 0.9685964547697703 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 8050 0.9614305130575401 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 6805 0.8127372225287652 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 6430 0.767950086827327 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 6071 0.7250738689158169 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 6023 0.7193411155460327 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 5272 0.6296474117812859 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 5269 0.6292891146956744 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 4583 0.5473585144525102 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 4308 0.5145146149381221 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 4082 0.48752290115538865 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3869 0.46208380807697175 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3842 0.4588591343064682 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3720 0.444288386158267 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 3470 0.4144302956906415 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3444 0.41132505428200844 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 3316 0.39603771196258425 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3272 0.39078268804028216 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3068 0.36641848621869977 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2918 0.3485036319381245 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2696 0.3219896476028731 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2625 0.3135099499100674 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 2311 0.2760081882827298 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 2297 0.2743361352165428 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2185 0.2609597106870466 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 2113 0.2523605806323705 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 2052 0.24507520655826986 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 2004 0.23934245318848577 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1955 0.23349026745683116 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1887 0.22536886684963706 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1837 0.21939724875611197 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1773 0.21175357759639982 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1722 0.20566252714100422 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1674 0.19992977377122015 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1670 0.19945204432373814 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1638 0.1956302087438821 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 1563 0.18667278160359443 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1438 0.17174373636978169 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1324 0.15812844711654447 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1155 0.13794437796042966 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 1060 0.126598303582732 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1014 0.1211044149366889 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 1005 0.1200295236798544 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 1002 0.11967122659424288 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1000 0.11943236187050188 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 982 0.11728257935683284 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 968 0.11561052629064583 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 853 0.10187580467553811 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.1943236187050188E-4 0.0 10-11 0.0 0.0 0.0 3.5829708561150567E-4 0.0 12-13 0.0 0.0 0.0 7.165941712230112E-4 0.0 14-15 0.0 0.0 0.0 7.165941712230112E-4 0.0 16-17 0.0 0.0 0.0 7.165941712230112E-4 0.0 18-19 0.0 0.0 0.0 0.0011346074377697679 0.0 20-21 0.0 0.0 0.0 0.0023886472374100376 0.0 22-23 0.0 0.0 0.0 0.0047175782938848245 0.0 24-25 0.0 0.0 0.0 0.008419981511870383 0.0 26-27 0.0 0.0 0.0 0.013137559805755206 0.0 28-29 0.0 0.0 0.0 0.03254531860971176 0.0 30-31 0.0 0.0 0.0 0.07661586013992695 0.0 32-33 0.0 0.0 0.0 0.1711465745604292 0.0 34-35 0.0 0.0 0.0 0.30341791533201 0.0 36-37 0.0 0.0 0.0 0.4667416701899213 0.0 38-39 0.0 0.0 0.0 0.6937228739248102 0.0 40-41 0.0 0.0 0.0 0.948292953251785 0.0 42-43 0.0 0.0 0.0 1.212955067156817 0.0 44-45 0.0 0.0 0.0 1.5043103139399063 0.0 46-47 0.0 0.0 0.0 1.8839260761452965 0.0 48-49 0.0 0.0 0.0 2.2057962913862994 0.0 50-51 0.0 0.0 0.0 2.555613679304999 0.0 52-53 0.0 0.0 0.0 2.884888700981973 0.0 54-55 0.0 0.0 0.0 3.2559650493136223 0.0 56-57 0.0 0.0 0.0 3.7848712638571396 0.0 58-59 0.0 0.0 0.0 4.26104809063483 0.0 60-61 0.0 0.0 0.0 4.839877032440218 0.0 62-63 0.0 0.0 0.0 5.383413711312873 0.0 64-65 0.0 0.0 0.0 5.886940548958908 0.0 66-67 0.0 0.0 0.0 6.490910002938036 0.0 68-69 0.0 0.0 0.0 7.199502205915724 0.0 70-71 0.0 0.0 0.0 7.918723889099885 0.0 72-73 0.0 0.0 0.0 8.606952874378653 0.0 74-75 0.0 0.0 0.0 9.441008773501302 0.0 76-77 0.0 0.0 0.0 10.130670947122516 0.0 78-79 0.0 0.0 0.0 11.174748654594444 0.0 80-81 0.0 0.0 0.0 12.237935539965651 0.0 82-83 0.0 0.0 0.0 13.105014487145494 0.0 84-85 0.0 0.0 0.0 14.164379536936847 0.0 86-87 0.0 0.0 0.0 15.228342732660213 0.0 88-89 0.0 0.0 0.0 16.29791924939149 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 480 0.0 41.52514 94-95 CATTCCG 100 1.3077915E-6 33.220108 9 CTCCGAC 155 0.0 32.14849 94-95 CGCCTTC 30 0.0038752225 31.79294 90-91 GTACAAC 90 2.3701677E-5 31.748167 1 TTACTCG 30 0.0039688977 31.6382 94-95 GATTGGC 260 0.0 27.379211 9 CTTTGCG 1065 0.0 27.182114 2 TTTGCGT 1085 0.0 26.681065 3 GCTTTGC 1100 0.0 26.4087 1 CGGTATA 165 8.477946E-8 25.8858 2 GCAAGCG 85 1.133285E-7 25.124454 94-95 ATATGAG 305 0.0 24.895634 3 TTGCGTT 1205 0.0 24.417864 4 ATGGGAT 615 0.0 23.921566 5 GTCCTAC 220 2.1027518E-9 23.811125 1 CTACGTG 120 1.7207357E-4 23.728651 4 AGATTGG 325 0.0 23.363594 8 GACAATG 330 0.0 23.0096 7 GAGTACT 2220 0.0 22.874247 12-13 >>END_MODULE