##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938962_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 849213 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00948407525556 33.0 31.0 34.0 30.0 34.0 2 32.19837072677879 33.0 31.0 34.0 30.0 34.0 3 32.324637046300516 34.0 31.0 34.0 30.0 34.0 4 35.83400159912766 37.0 35.0 37.0 35.0 37.0 5 35.76699956312492 37.0 35.0 37.0 35.0 37.0 6 35.879279992181 37.0 35.0 37.0 35.0 37.0 7 35.84022147564863 37.0 35.0 37.0 35.0 37.0 8 35.84720912185753 37.0 35.0 37.0 35.0 37.0 9 37.43647117978646 39.0 37.0 39.0 34.0 39.0 10-11 37.33581857555171 39.0 37.0 39.0 33.5 39.0 12-13 37.327591546526016 39.0 37.0 39.0 34.0 39.0 14-15 38.29215167455044 40.0 37.5 41.0 33.0 41.0 16-17 38.39183573496873 40.0 38.0 41.0 33.5 41.0 18-19 38.62803501595006 40.0 38.0 41.0 34.0 41.0 20-21 38.60285876452669 40.0 38.0 41.0 34.0 41.0 22-23 38.57233874187041 40.0 38.0 41.0 34.0 41.0 24-25 38.43552442084612 40.0 38.0 41.0 34.0 41.0 26-27 38.16087483352233 40.0 37.5 41.0 33.5 41.0 28-29 38.03929697260875 40.0 37.0 41.0 33.0 41.0 30-31 38.04479971455925 40.0 37.0 41.0 33.0 41.0 32-33 37.884549577079014 40.0 37.0 41.0 33.0 41.0 34-35 37.7673339904123 40.0 37.0 41.0 33.0 41.0 36-37 37.61213264516676 40.0 36.5 41.0 32.5 41.0 38-39 37.447937089988024 40.0 37.0 41.0 32.0 41.0 40-41 37.49082150179048 40.0 37.0 41.0 32.5 41.0 42-43 37.35127759466706 40.0 37.0 41.0 32.0 41.0 44-45 37.15719260067851 40.0 36.0 41.0 31.0 41.0 46-47 36.92198188204844 40.0 36.0 41.0 31.0 41.0 48-49 36.6347017768216 39.0 36.0 41.0 30.0 41.0 50-51 36.395801171202045 39.0 36.0 41.0 30.0 41.0 52-53 36.28272883246017 39.0 35.0 40.5 29.5 41.0 54-55 36.17516688981445 39.0 35.0 40.0 29.0 41.0 56-57 35.85327709302613 38.5 35.0 40.0 28.5 41.0 58-59 35.60399511076726 38.5 35.0 40.0 28.0 41.0 60-61 34.94805249095339 38.0 34.0 40.0 26.5 41.0 62-63 34.746781431749156 38.0 34.0 40.0 26.5 41.0 64-65 34.47816978779176 37.0 34.0 39.5 26.0 41.0 66-67 33.88220564216516 36.5 33.0 39.0 26.0 40.0 68-69 33.379282347302734 36.0 32.5 39.0 25.5 40.0 70-71 32.7113551017236 35.5 32.0 37.5 24.0 39.5 72-73 32.07499296407379 35.0 31.5 37.0 22.5 39.0 74-75 31.66560568432184 35.0 31.0 37.0 22.0 39.0 76-77 29.652399927933274 32.5 28.5 34.5 20.0 36.0 78-79 30.639356086164483 34.0 30.0 35.0 20.0 37.0 80-81 30.591038997283366 34.0 30.5 35.0 20.0 37.0 82-83 30.254376699367533 34.0 30.0 35.0 18.0 36.0 84-85 29.854551213888627 34.0 30.0 35.0 15.5 36.0 86-87 29.46542622404509 34.0 29.0 35.0 7.5 35.0 88-89 28.820565040808372 34.0 29.0 35.0 2.0 35.0 90-91 28.25331866092488 33.0 27.5 35.0 2.0 35.0 92-93 27.985238096920327 33.0 27.0 35.0 2.0 35.0 94-95 27.59217357718264 33.0 27.0 35.0 2.0 35.0 96-97 26.854880342152086 33.0 25.0 34.0 2.0 35.0 98-99 26.03111940113964 32.0 25.0 34.0 2.0 35.0 100-101 21.695004080248417 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19574137513775725 1101 2 0.3243692994198497 1101 3 0.2906773840541774 1101 4 0.13214404204921237 1101 5 0.16924764008787463 1101 6 0.08533055116171795 1101 7 0.126712813999859 1101 8 0.1415592765380964 1101 9 0.28456208527320825 1101 10-11 0.35151764714994727 1101 12-13 0.2576554209216013 1101 14-15 0.28759905204083935 1101 16-17 0.3780238500022577 1101 18-19 0.3946093312893808 1101 20-21 0.39854115807529666 1101 22-23 0.35018433275015326 1101 24-25 0.37349188214776774 1101 26-27 0.35985066592343884 1101 28-29 0.4530011341647153 1101 30-31 0.4027063066174392 1101 32-33 0.4251827177973979 1101 34-35 0.5199576034970121 1101 36-37 0.47178375434109654 1101 38-39 0.5651551561576866 1101 40-41 0.6110175918490341 1101 42-43 0.3789638466872489 1101 44-45 0.5272906752932798 1101 46-47 0.39230191744140086 1101 48-49 0.399945271299039 1101 50-51 0.5526136891202711 1101 52-53 0.6954264236721315 1101 54-55 0.7085510630140988 1101 56-57 0.4997148202192747 1101 58-59 0.5847697383643649 1101 60-61 0.8569050385859285 1101 62-63 0.7385254741546561 1101 64-65 0.6471367678754163 1101 66-67 0.8359687270943326 1101 68-69 0.7293495019616998 1101 70-71 0.9068277700516845 1101 72-73 1.2120052665848462 1101 74-75 1.110373685292064 1101 76-77 0.8729272110696904 1101 78-79 1.0715268880217863 1101 80-81 1.537870542439709 1101 82-83 1.4711792368076857 1101 84-85 1.3558445001316493 1101 86-87 1.2632299905801503 1101 88-89 1.3354308339073349 1101 90-91 1.3027030585552133 1101 92-93 1.5727611010947733 1101 94-95 1.4221801772663483 1101 96-97 1.31161248144765 1101 98-99 1.4131351960996064 1101 100-101 1.9875732340498509 1102 1 0.11348899356396203 1102 2 0.1292466320571961 1102 3 0.144970741746981 1102 4 0.1871453470211506 1102 5 0.10376414798295741 1102 6 0.14792679284251875 1102 7 0.10165735269227838 1102 8 0.2665592765380964 1102 9 0.25284147976622506 1102 10-11 0.25909790292445223 1102 12-13 0.22586304195775142 1102 14-15 0.3602223718894635 1102 16-17 0.4800400316543545 1102 18-19 0.2750673764414131 1102 20-21 0.393751911044113 1102 22-23 0.46770194987138325 1102 24-25 0.4199195214535152 1102 26-27 0.45862236108199284 1102 28-29 0.5520746040851137 1102 30-31 0.4525187169006202 1102 32-33 0.4069718343313937 1102 34-35 0.57792608842459 1102 36-37 0.5475210634889507 1102 38-39 0.49902407172600505 1102 40-41 0.5768713697428112 1102 42-43 0.4888881583145519 1102 44-45 0.508849790522298 1102 46-47 0.4953229274896813 1102 48-49 0.553827562829774 1102 50-51 0.5457315933353257 1102 52-53 0.2764929119926336 1102 54-55 0.4741846268924732 1102 56-57 0.5180219453660797 1102 58-59 0.6327470318525599 1102 60-61 0.39959882691947257 1102 62-63 0.4049974825064737 1102 64-65 0.26301175156326195 1102 66-67 0.30719083331905495 1102 68-69 0.446765983757345 1102 70-71 0.4372629781947097 1102 72-73 0.6697437502074521 1102 74-75 0.6471706316577119 1102 76-77 0.2919716453643524 1102 78-79 0.46296627207502894 1102 80-81 0.6635931706532041 1102 82-83 0.8296729729423582 1102 84-85 0.8803926535963491 1102 86-87 0.892639164532909 1102 88-89 1.0942230823739898 1102 90-91 0.8720884167700085 1102 92-93 0.988208706471255 1102 94-95 0.9714249247542774 1102 96-97 0.8531676817608442 1102 98-99 1.321514747189255 1102 100-101 1.612653163581367 1103 1 -0.014152841725795895 1103 2 0.01494631311803829 1103 3 0.01598896495146107 1103 4 0.0637863618035226 1103 5 0.047757901539590364 1103 6 0.048958051527328905 1103 7 0.0235701936329491 1103 8 0.05811896845080611 1103 9 -0.16324344274876523 1103 10-11 0.026981159280069278 1103 12-13 -0.04588205815738178 1103 14-15 -0.13401174401382576 1103 16-17 3.2443010332627864E-4 1103 18-19 6.142710856167355E-4 1103 20-21 -0.004895575159459042 1103 22-23 0.07080879449110711 1103 24-25 0.15662101557101238 1103 26-27 0.017114116306110816 1103 28-29 -0.058809691138741016 1103 30-31 -0.07325199760251166 1103 32-33 0.14332428195788083 1103 34-35 -0.09490403346045895 1103 36-37 -0.13297567433247082 1103 38-39 0.06327592117613534 1103 40-41 -0.008913835979562634 1103 42-43 0.022720854210689367 1103 44-45 -0.03816974946419549 1103 46-47 0.020523753290667912 1103 48-49 -0.14342175231123377 1103 50-51 -0.11988269540386653 1103 52-53 0.010343308350641678 1103 54-55 0.13386264391138525 1103 56-57 -0.15627549518021766 1103 58-59 -0.06743692061095174 1103 60-61 0.12074459270431959 1103 62-63 0.10314174623342609 1103 64-65 0.3446985559516591 1103 66-67 0.16444405248716265 1103 68-69 0.23030008775004518 1103 70-71 0.31974679964171315 1103 72-73 0.33870210284035096 1103 74-75 0.3806926596343736 1103 76-77 0.3234098161622292 1103 78-79 0.47005307381661865 1103 80-81 0.5410952219216831 1103 82-83 0.7295785153374439 1103 84-85 0.8657054058692886 1103 86-87 0.7144161916493559 1103 88-89 0.9626590232445231 1103 90-91 1.0266415710915702 1103 92-93 0.9347417318937481 1103 94-95 0.7436869776108352 1103 96-97 0.6770274080819298 1103 98-99 0.5696479274494344 1103 100-101 0.6009220584108483 1104 1 0.09447555235294658 1104 2 0.010445248786936645 1104 3 0.12232295367442703 1104 4 0.16758708002389255 1104 5 0.057855235024582896 1104 6 0.016976120781968973 1104 7 0.13810521906314932 1104 8 0.0858630740064541 1104 9 0.14658740172890106 1104 10-11 0.08189739398539331 1104 12-13 0.1855035221874246 1104 14-15 0.28506740647122086 1104 16-17 0.37485929304023813 1104 18-19 0.30726755913748605 1104 20-21 0.11562976567022787 1104 22-23 -0.017537186237191804 1104 24-25 0.28425137581864846 1104 26-27 0.2788380076955903 1104 28-29 0.16249480505079106 1104 30-31 0.04764301547820082 1104 32-33 0.11568904691132076 1104 34-35 0.0421095022311917 1104 36-37 -0.04530485325383182 1104 38-39 0.1689526245121229 1104 40-41 0.036334047545238946 1104 42-43 0.03655878339611007 1104 44-45 -0.12334223609912698 1104 46-47 -0.18174871546999327 1104 48-49 0.029692106742075453 1104 50-51 -0.07523441214554794 1104 52-53 -0.03621914594812381 1104 54-55 0.13450043010270463 1104 56-57 0.05920849110535187 1104 58-59 0.08033935861752894 1104 60-61 0.2138670639023772 1104 62-63 0.4290318032685718 1104 64-65 0.35156714762224794 1104 66-67 0.3885003726639624 1104 68-69 0.3090963374047462 1104 70-71 -0.049501343872357495 1104 72-73 0.1278280513949781 1104 74-75 0.12113317896294973 1104 76-77 0.024193033854498225 1104 78-79 0.1683623310597575 1104 80-81 0.44293383357895166 1104 82-83 0.5540906292127481 1104 84-85 0.4811609558278498 1104 86-87 0.5119641677953375 1104 88-89 0.5645447579579645 1104 90-91 0.5546017927324272 1104 92-93 0.5107357846390741 1104 94-95 0.685471316506856 1104 96-97 0.8166757725868905 1104 98-99 0.8473124112894759 1104 100-101 1.265421335315672 1105 1 -0.05520143927504506 1105 2 -0.14066716429374537 1105 3 -0.07689535064725561 1105 4 -0.05944386402396162 1105 5 -0.013693817535582298 1105 6 0.0341025026256645 1105 7 0.04995399872364459 1105 8 0.017320543285961776 1105 9 -0.03699082180214219 1105 10-11 -0.11152834492516206 1105 12-13 -0.19111807234840938 1105 14-15 0.03729223263258774 1105 16-17 -0.03454803670583573 1105 18-19 -0.06169819734966353 1105 20-21 -0.10892272511747336 1105 22-23 -0.16469332493806377 1105 24-25 0.04763810703559557 1105 26-27 0.037765064850347585 1105 28-29 0.11640713795755886 1105 30-31 0.09852261584206445 1105 32-33 0.09915009318092416 1105 34-35 -0.03781078639490687 1105 36-37 0.020597334568982717 1105 38-39 0.07914935896685904 1105 40-41 -0.02227616846179359 1105 42-43 -0.08987548170202331 1105 44-45 -0.05317618358520804 1105 46-47 0.0452274944623241 1105 48-49 -0.35099368859858515 1105 50-51 -0.08484448939003641 1105 52-53 -0.03283488162088588 1105 54-55 0.106142761930208 1105 56-57 -0.03759997032637585 1105 58-59 -0.250504471845467 1105 60-61 -0.3493801338650897 1105 62-63 -0.23834512206175873 1105 64-65 -0.1827734990112191 1105 66-67 -0.1781881362553932 1105 68-69 -0.26809880898974114 1105 70-71 -0.2761067494444518 1105 72-73 -0.016884324682358454 1105 74-75 0.007956133371745722 1105 76-77 -0.26606120996671123 1105 78-79 0.1088971835860093 1105 80-81 -0.10728201927107506 1105 82-83 -0.10528894802402533 1105 84-85 -0.3207285275129337 1105 86-87 -0.43688024855397245 1105 88-89 -0.30385978111910816 1105 90-91 -0.2820484793963409 1105 92-93 0.22601431190591725 1105 94-95 0.5589792336810326 1105 96-97 0.2183332141138976 1105 98-99 0.5449449680664884 1105 100-101 0.8068334754966813 1106 1 -0.1569224855435003 1106 2 -0.34595662256541004 1106 3 -0.22863228094872312 1106 4 -0.2603987127191374 1106 5 -0.11615862800368859 1106 6 -0.11914755119937581 1106 7 -0.12965150513143442 1106 8 -0.17364467896252478 1106 9 -0.2569628478369026 1106 10-11 -0.19579502203063015 1106 12-13 -0.2946360469008411 1106 14-15 -0.25979263327155167 1106 16-17 -0.16357305819820311 1106 18-19 -0.28400389648047764 1106 20-21 -0.2280160855916833 1106 22-23 0.09010312607211546 1106 24-25 0.04265759696993854 1106 26-27 0.021929963696905475 1106 28-29 -0.008083029750729054 1106 30-31 -0.08867937033957674 1106 32-33 -0.09265224019105034 1106 34-35 -0.31698282929233557 1106 36-37 -0.20795056369491505 1106 38-39 -0.13932685789310284 1106 40-41 -0.15253954229063282 1106 42-43 0.01226562572372103 1106 44-45 -0.07756607863014864 1106 46-47 0.024304233161700495 1106 48-49 -0.3531308814759129 1106 50-51 -0.2838714229887529 1106 52-53 -0.16743286502621402 1106 54-55 -0.20161150680956297 1106 56-57 -0.2700556418295079 1106 58-59 -0.10937446216448876 1106 60-61 -0.04045300619510073 1106 62-63 0.003759158496940529 1106 64-65 -0.2567976096182889 1106 66-67 -0.02556215178410781 1106 68-69 0.23552214829800988 1106 70-71 0.09363364071558067 1106 72-73 0.12747443464668606 1106 74-75 -0.13212447621377876 1106 76-77 -0.3224984590027553 1106 78-79 -0.18755683778441679 1106 80-81 -0.15863788333988893 1106 82-83 0.023480875023331294 1106 84-85 -0.1460041644174872 1106 86-87 -0.10883506864283277 1106 88-89 -0.23565630289227713 1106 90-91 0.01407355815621969 1106 92-93 0.11224022049518823 1106 94-95 -0.19357689115692978 1106 96-97 -0.19732181781225577 1106 98-99 -0.20351217289790569 1106 100-101 0.17566448594702067 1107 1 -0.21179328429563427 1107 2 -0.29478380485983635 1107 3 -0.11515444234727568 1107 4 -0.18554733830039538 1107 5 -0.2046673689537144 1107 6 -0.10928970200284027 1107 7 -0.23449875923703445 1107 8 -0.11677405679523645 1107 9 -0.29292435233981706 1107 10-11 -0.22400224435095595 1107 12-13 -0.14382890698677642 1107 14-15 -0.2973316290923762 1107 16-17 -0.31765879014240994 1107 18-19 -0.26387469523261586 1107 20-21 -0.33242629219595443 1107 22-23 -0.4392445400647915 1107 24-25 -0.44873536318075224 1107 26-27 -0.4339709133411773 1107 28-29 -0.4069521509468004 1107 30-31 -0.27082443479304885 1107 32-33 -0.25469974151544506 1107 34-35 -0.35267651344090467 1107 36-37 -0.4577747146161002 1107 38-39 -0.5990552115336882 1107 40-41 -0.7153537161678472 1107 42-43 -0.6104122835116641 1107 44-45 -0.6106764735915888 1107 46-47 -0.7727674014253907 1107 48-49 -0.7652446021186847 1107 50-51 -0.5498501445144441 1107 52-53 -0.400949405140409 1107 54-55 -0.6895426681492509 1107 56-57 -0.40668964029911336 1107 58-59 -0.3400192911714939 1107 60-61 -0.5065744671404175 1107 62-63 -0.6006848935367302 1107 64-65 -0.7714813755845071 1107 66-67 -0.6659375417423163 1107 68-69 -0.5330450128062267 1107 70-71 -0.4991999574950299 1107 72-73 -0.6216897304012861 1107 74-75 -0.6405380084993766 1107 76-77 -0.9314880993582797 1107 78-79 -0.9224227804531999 1107 80-81 -0.7218265642510637 1107 82-83 -0.641878327990387 1107 84-85 -0.6625626728641301 1107 86-87 -0.570449937087119 1107 88-89 -0.43048236681599406 1107 90-91 -0.2700139396364669 1107 92-93 0.24033192689344318 1107 94-95 0.1848760784116159 1107 96-97 0.23088614027827248 1107 98-99 -0.27097420715536913 1107 100-101 0.3259999971601566 1108 1 0.05349644605433923 1108 2 -0.04511369585237901 1108 3 0.03583523063035443 1108 4 -0.07398681047480693 1108 5 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2175.0 14 2691.0 15 3522.0 16 4439.0 17 6356.0 18 6016.0 19 4637.0 20 4712.0 21 5069.0 22 5521.0 23 6234.0 24 7440.0 25 8688.0 26 10270.0 27 12303.0 28 15013.0 29 18592.0 30 22482.0 31 27727.0 32 34119.0 33 43555.0 34 58191.0 35 83694.0 36 127857.0 37 189629.0 38 125999.0 39 8024.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.3861219703355 19.836699857079868 14.849508290904534 17.927669881680096 2 13.69774132049321 26.87759136989189 34.85662607614344 24.56804123347146 3 28.094011749702368 25.162944985533663 30.77602438964076 15.967018875123202 4 11.534090975997776 16.686626323431224 42.73615688878997 29.043125811781024 5 10.278977260014909 42.28723435573575 35.11043806648305 12.323350317766298 6 25.414118719331903 39.60349170349488 15.882352248493605 19.100037328679612 7 23.563228542191418 35.523478797427735 22.54251877915199 18.370773881228857 8 22.27697880272676 27.222145680765603 20.73602264685067 29.764852869656966 9 19.957654911076492 24.625506203979448 26.594505736487783 28.822333148456273 10-11 24.550495576492587 24.081826349808587 27.749398560785103 23.61827951291372 12-13 32.026181888407265 23.32465470971358 25.4015188180115 19.24764458386765 14-15 23.889298749594474 27.09176647085596 31.320312127408652 17.698622652140916 16-17 18.202923843354345 29.57734454165467 31.336784855694923 20.882946759296058 18-19 17.51127220143827 34.95624772583557 28.626269263423897 18.90621080930226 20-21 21.93572166229203 27.644419009129628 25.374434917976995 25.045424410601345 22-23 18.933471343467424 27.943519470380224 27.95394088408915 25.169068302063202 24-25 24.928021591756135 28.13722823367047 26.615760710210512 20.318989464362886 26-27 23.54927444586929 23.567821029588572 33.962798496961305 18.92010602758083 28-29 18.310188374412544 32.95298117197923 29.240956038120004 19.495874415488224 30-31 20.088717436026062 28.281538318419525 27.66820573872515 23.961538506829264 32-33 21.072216275539823 28.72341803528679 29.378141879599113 20.826223809574277 34-35 24.71225724096675 25.930703865774213 28.283282069157178 21.073756824101856 36-37 22.468062255049745 24.937113732288214 33.11262294618631 19.48220106647573 38-39 21.478548126862243 27.779439648573213 31.678345561823555 19.06366666274099 40-41 20.10726654600309 29.614999181575918 29.991886414513548 20.28584785790745 42-43 18.550461135552183 30.653561907094613 26.128993132424565 24.66698382492864 44-45 21.499662628015887 26.17676878891112 27.12441313171874 25.199155451354255 46-47 24.45890886824225 29.377951272359017 27.113729230696766 19.04941062870197 48-49 22.632321121038608 27.561476073420966 28.779486877548315 21.02671592799211 50-51 17.407081177896135 29.210359287289698 33.418628394188005 19.96393114062616 52-53 20.44846228213652 31.818165760533578 26.731397187749128 21.001974769580777 54-55 17.596586486546954 26.94176843736495 29.77268364944955 25.688961426638546 56-57 22.763017052258974 26.510369012250166 27.38559112966947 23.34102280582139 58-59 26.130989670423876 25.907193963344845 26.798431958097623 21.16338440813366 60-61 20.925926143997888 27.804633340124095 31.970058632136627 19.299381883741386 62-63 20.179095232880325 27.648717106309018 30.676108349730868 21.49607931107979 64-65 19.090143138833067 32.331971872698226 28.169517451797876 20.408367536670827 66-67 20.95685688950856 27.095008578692575 26.28954176710469 25.658592764694177 68-69 18.39124449631007 28.857705401417565 28.585567879529393 24.165482222742973 70-71 23.707715261071133 28.24232554141305 25.668707379656226 22.381251817859596 72-73 21.528462235034084 24.566039379990652 31.432867843521002 22.472630541454265 74-75 18.56230415690763 32.78741611350745 26.976329848930714 21.673949880654206 76-77 21.038009127661105 26.305926377431497 27.245101633948014 25.410962860959387 78-79 20.786858876228788 27.082401096545976 29.58978441190186 22.540955615323384 80-81 23.083490243319403 27.27272192017786 27.642829301953693 22.00095853454905 82-83 20.708173332249977 26.174057627473907 30.88977676978567 22.227992270490443 84-85 19.756998538646958 28.007873171983945 29.203156334158802 23.031971955210295 86-87 20.160930844665828 28.999627360248304 27.966967136947467 22.872474658138405 88-89 20.01260696256761 28.058711074081277 26.067851713263746 25.860830250087368 90-91 21.107138067943172 24.537732521973986 28.23634493681168 26.118784473271162 92-93 22.66704584126715 28.665776430648144 28.072109117500553 20.595068610584153 94-95 21.171720169144844 27.330657915034273 28.236614371188384 23.261007544632502 96-97 16.62346960524441 27.846384105679522 31.24990432272856 24.280241966347507 98-99 20.417845699488822 29.258913841403743 25.71192386362432 24.611316595483114 100-101 19.61014400425807 26.205206950922683 27.975143986594613 26.209505058224636 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3655.0 1 2290.5 2 827.5 3 677.5 4 533.0 5 544.0 6 900.0 7 1589.0 8 2082.0 9 2013.5 10 1536.0 11 1060.5 12 935.5 13 879.0 14 811.0 15 818.5 16 810.5 17 777.5 18 777.5 19 879.0 20 1008.0 21 1158.0 22 1341.0 23 1504.5 24 1757.0 25 2209.0 26 2939.5 27 3694.5 28 4217.5 29 5028.0 30 6575.0 31 8022.5 32 10019.0 33 12745.0 34 15111.0 35 17283.5 36 20078.5 37 22374.0 38 22369.5 39 21677.0 40 20691.0 41 19468.0 42 18345.5 43 18342.0 44 19291.5 45 20451.0 46 27871.0 47 65769.0 48 143604.5 49 140868.0 50 67134.0 51 30795.0 52 14482.0 53 8365.5 54 6168.5 55 5060.5 56 4214.0 57 3425.0 58 2820.5 59 2329.0 60 1812.5 61 1385.0 62 985.0 63 587.0 64 326.0 65 218.0 66 161.5 67 113.0 68 69.5 69 57.5 70 48.5 71 39.5 72 43.0 73 49.5 74 42.0 75 34.0 76 21.5 77 10.0 78 6.5 79 5.5 80 4.0 81 4.5 82 3.5 83 2.0 84 2.0 85 1.5 86 0.5 87 1.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38694650223206667 2 0.0 3 0.0 4 0.0 5 4.7102434842613105E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0010009267404055284 16-17 0.002649511959896987 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.002001853480811057 36-37 0.007183121313498499 38-39 0.012128876971972874 40-41 0.002355121742130655 42-43 5.887804355326638E-4 44-45 2.3551217421306553E-4 46-47 3.532682613195983E-4 48-49 5.887804355326638E-5 50-51 4.7102434842613105E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.1775608710653276E-4 60-61 0.002355121742130655 62-63 0.0 64-65 2.943902177663319E-4 66-67 0.0018840973937045242 68-69 0.0011775608710653275 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.12034672102287647 78-79 1.1775608710653276E-4 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.1423671093117981 88-89 0.42963308380818477 90-91 0.3314245071613364 92-93 0.0 94-95 0.0 96-97 4.7102434842613105E-4 98-99 0.0 100-101 2.3551217421306553E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 849213.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.430880429097858 #Duplication Level Percentage of deduplicated Percentage of total 1 75.62045907643757 19.98715311842809 2 12.29192201415499 6.497726419998524 3 4.371930280859928 3.466618994932829 4 2.033830642537065 2.1502373810372974 5 1.1742673679864357 1.5518460197520467 6 0.7662925560853004 1.2152272154159005 7 0.5058639615986726 0.9359300917682987 8 0.38943025199903764 0.8234387540848009 9 0.30389565533268387 0.7229006756118451 >10 2.120613359555821 11.125737612423928 >50 0.2491463104533139 4.589311107269531 >100 0.13886111568070303 6.55293864046759 >500 0.007590478992192771 1.3690752457014514 >1k 0.016966953041372076 10.494709374169355 >5k 0.0044649876424663355 8.551852226511137 >10k+ 0.0044649876424663355 19.96529712242739 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 45938 5.409479129499902 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 23829 2.806009799661569 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 17196 2.024933673883937 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 14151 1.666366388644545 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 12283 1.446398017929542 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 11876 1.398471290477183 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 11551 1.36020056216756 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 11287 1.3291129551714351 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 10748 1.265642424221014 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 10319 1.2151250628523114 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 9957 1.1724973593197467 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 9257 1.0900680983451738 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 8370 0.9856184490816792 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 8095 0.9532355251273825 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 6926 0.815578659299846 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 6614 0.7788387601226077 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 6319 0.7441007144261805 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 6117 0.7203139848306609 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 5584 0.6575499904028789 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 5226 0.6153933112187402 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 4534 0.5339060989410195 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 4480 0.5275472702372668 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 4175 0.4916316636697743 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3927 0.46242815406735416 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3857 0.45418522796989685 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3845 0.4527721549246184 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3480 0.409791183130734 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 3293 0.38777079484181237 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3264 0.3843558683157229 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 3126 0.3681055282950214 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3104 0.3655148943786777 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 3043 0.35833177306517916 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2699 0.3178236791005319 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2669 0.31429099648733594 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 2331 0.27448943904532785 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 2287 0.26930817121264045 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2239 0.2636558790315268 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 2198 0.258827879460159 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 2124 0.25011392901427554 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1997 0.2351589059517459 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1961 0.23091968681591074 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1950 0.22962436985773885 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1939 0.228329052899567 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1860 0.21902632201815092 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1834 0.21596466375338108 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1713 0.20171617721349058 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1656 0.19500408024841825 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1604 0.18888076371887855 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 1523 0.17934252066324938 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1410 0.16603608282021118 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1345 0.15838193715828655 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1187 0.1397764753954544 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 1092 0.12858964712033377 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1067 0.12564574494267045 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 1035 0.1218775501552614 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 1034 0.12175979406815486 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 1028 0.12105325754551567 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1018 0.11987569667445035 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 939 0.11057296579303426 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.7663413065979912E-4 0.0 12-13 0.0 0.0 0.0 2.355121742130655E-4 0.0 14-15 0.0 0.0 0.0 5.299023919793974E-4 0.0 16-17 0.0 0.0 0.0 9.42048696852262E-4 0.0 18-19 0.0 0.0 0.0 0.0012953169581718603 0.0 20-21 0.0 0.0 0.0 0.0021196095679175894 0.0 22-23 0.0 0.0 0.0 0.004651365440708044 0.0 24-25 0.0 0.0 0.0 0.008007413923244227 0.0 26-27 0.0 0.0 0.0 0.012364389146185938 0.0 28-29 0.0 0.0 0.0 0.02961565590729299 0.0 30-31 0.0 0.0 0.0 0.07954423684046288 0.0 32-33 0.0 0.0 0.0 0.17445564304832828 0.0 34-35 0.0 0.0 0.0 0.30728450930449724 0.0 36-37 0.0 0.0 0.0 0.4791495184364818 0.0 38-39 0.0 0.0 0.0 0.726319545273094 0.0 40-41 0.0 0.0 0.0 0.984676400384827 0.0 42-43 0.0 0.0 0.0 1.252571498552189 0.0 44-45 0.0 0.0 0.0 1.5489046917557787 0.0 46-47 0.0 0.0 0.0 1.941562364212512 0.0 48-49 0.0 0.0 0.0 2.290532528352722 0.0 50-51 0.0 0.0 0.0 2.6631127879577914 0.0 52-53 0.0 0.0 0.0 3.0039577820876504 0.0 54-55 0.0 0.0 0.0 3.3810716510463217 0.0 56-57 0.0 0.0 0.0 3.9278720415255064 0.0 58-59 0.0 0.0 0.0 4.407728096484627 0.0 60-61 0.0 0.0 0.0 5.0002767268047 0.0 62-63 0.0 0.0 0.0 5.556085457947535 0.0 64-65 0.0 0.0 0.0 6.059845998589282 0.0 66-67 0.0 0.0 0.0 6.662286140226303 0.0 68-69 0.0 0.0 0.0 7.367409589820221 0.0 70-71 0.0 0.0 0.0 8.108330889894527 0.0 72-73 0.0 0.0 0.0 8.803445072084388 0.0 74-75 0.0 0.0 0.0 9.644459046199245 0.0 76-77 0.0 0.0 0.0 10.327562107504242 0.0 78-79 0.0 0.0 0.0 11.376003429057256 0.0 80-81 0.0 0.0 0.0 12.448996894771984 0.0 82-83 0.0 0.0 0.0 13.309381745215864 0.0 84-85 0.0 0.0 0.0 14.383317259627443 0.0 86-87 0.0 0.0 0.0 15.461491993174857 0.0 88-89 0.0 0.0 0.0 16.559508627399723 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTACGG 15 6.641672E-4 94.91226 8 GCGTGCA 25 0.005039219 56.94736 9 CATTCCG 75 1.3721728E-7 44.292393 9 GTAAGCG 170 0.0 32.10268 94-95 CTTTGCG 900 0.0 30.055552 2 TGGCCCG 65 0.005869662 29.203777 5 GCAGCGC 65 0.005869662 29.203777 8 TTTGCGT 935 0.0 28.930477 3 CTCCGAC 75 3.3687684E-8 28.47368 94-95 TTGCGTT 980 0.0 28.086285 4 GCTTTGC 965 0.0 27.643478 1 CTATTAC 70 0.008274448 27.220362 1 GTCCTAT 90 8.797891E-4 26.464243 1 CTGTTCG 45 8.98844E-4 26.369179 36-37 GCTTATG 245 1.0913936E-11 25.276054 1 CTTAGAC 115 1.2894301E-4 24.759724 3 CGCAGTC 50 0.0016628876 23.728067 40-41 CTTATGT 240 2.3283064E-10 23.728065 2 CATGGGG 375 0.0 22.778944 4 TCTAAAC 235 4.6948117E-9 22.21351 3 >>END_MODULE