##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938961_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 214233 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.933534982939136 31.0 31.0 34.0 30.0 34.0 2 32.14347929590679 33.0 31.0 34.0 30.0 34.0 3 32.2195039979835 34.0 31.0 34.0 30.0 34.0 4 35.75586861034481 37.0 35.0 37.0 35.0 37.0 5 35.68486180933843 37.0 35.0 37.0 33.0 37.0 6 35.8126385757563 37.0 35.0 37.0 33.0 37.0 7 35.78285791638076 37.0 35.0 37.0 33.0 37.0 8 35.805982271638825 37.0 35.0 37.0 33.0 37.0 9 37.345553672870196 39.0 37.0 39.0 34.0 39.0 10-11 37.24166911726952 39.0 37.0 39.0 33.5 39.0 12-13 37.22933908408135 39.0 37.0 39.0 33.0 39.0 14-15 38.21275900538199 40.0 37.5 41.0 33.0 41.0 16-17 38.387823071142165 40.0 38.0 41.0 33.5 41.0 18-19 38.43451289017098 40.0 38.0 41.0 34.0 41.0 20-21 38.38340965210776 40.0 38.0 41.0 34.0 41.0 22-23 38.31685828047033 40.0 37.5 41.0 33.5 41.0 24-25 38.12148921968138 40.0 37.0 41.0 33.0 41.0 26-27 37.82560343177755 39.5 36.5 41.0 33.0 41.0 28-29 37.714593456657 40.0 36.5 41.0 33.0 41.0 30-31 37.632500595146404 40.0 36.0 41.0 33.0 41.0 32-33 37.37928797150765 39.5 35.5 41.0 33.0 41.0 34-35 37.10954894904147 39.0 35.0 41.0 31.5 41.0 36-37 36.894724435544475 39.0 35.0 41.0 31.0 41.0 38-39 36.63795960472943 39.0 35.0 41.0 30.5 41.0 40-41 36.688077933838386 39.0 35.0 41.0 31.0 41.0 42-43 36.42150602381519 39.0 35.0 41.0 30.0 41.0 44-45 36.16325682784632 39.0 35.0 41.0 30.0 41.0 46-47 35.811063188210966 39.0 35.0 40.0 29.0 41.0 48-49 35.369868320940284 38.5 35.0 40.0 27.0 41.0 50-51 35.072281114487495 38.0 35.0 40.0 27.0 41.0 52-53 34.968555264595096 38.0 34.5 40.0 26.5 41.0 54-55 34.779310377019414 38.0 34.0 40.0 25.5 41.0 56-57 34.529141168727506 38.0 34.0 40.0 24.0 41.0 58-59 34.25785009779072 38.0 34.0 40.0 23.0 41.0 60-61 33.647351248407105 37.5 33.0 40.0 22.0 41.0 62-63 33.25320328800885 37.0 33.0 40.0 21.0 41.0 64-65 32.82102430531244 36.5 32.0 39.0 19.0 40.5 66-67 32.14948210593139 36.0 31.5 39.0 15.0 40.0 68-69 31.52617243841985 35.0 31.0 38.0 10.5 39.0 70-71 30.751826282598852 35.0 30.5 37.0 7.5 39.0 72-73 30.114221431805557 34.0 29.5 36.5 2.0 38.5 74-75 29.662650011902926 34.0 29.0 36.0 2.0 37.0 76-77 27.796100507391486 31.5 26.5 34.0 2.0 36.0 78-79 28.720341870766873 33.5 29.0 35.0 2.0 36.0 80-81 28.619932503395837 34.0 29.0 35.0 2.0 36.0 82-83 28.17396246143218 33.5 28.0 35.0 2.0 35.5 84-85 27.678343205761948 33.0 26.5 35.0 2.0 35.0 86-87 27.21055813063347 33.0 26.0 35.0 2.0 35.0 88-89 26.459175290454787 32.5 25.0 34.5 2.0 35.0 90-91 25.801242105557968 32.0 23.5 34.0 2.0 35.0 92-93 25.45548771664496 32.0 22.0 34.0 2.0 35.0 94-95 24.830768369018777 31.0 19.0 34.0 2.0 35.0 96-97 23.835606559213566 31.0 4.5 34.0 2.0 35.0 98-99 22.723058539067278 31.0 2.0 34.0 2.0 35.0 100-101 17.457357176532092 23.5 2.0 30.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.02843593999757843 1101 2 0.14339574431104296 1101 3 0.19717002291481123 1101 4 0.09355836354502145 1101 5 0.19401394295319818 1101 6 0.2002632003193483 1101 7 0.22310486224490234 1101 8 0.17890641609629654 1101 9 0.41345735209293366 1101 10-11 0.4249775984898605 1101 12-13 0.3607622426398436 1101 14-15 0.4543396085441884 1101 16-17 0.08069231440989455 1101 18-19 0.17376694728572772 1101 20-21 0.4086479528388338 1101 22-23 0.27417949891181337 1101 24-25 0.3077812962486064 1101 26-27 0.13677686570004965 1101 28-29 0.1577109252223181 1101 30-31 0.33987241882355335 1101 32-33 0.33596250128880456 1101 34-35 0.5357483170135353 1101 36-37 0.3548597473494297 1101 38-39 -0.054300038599890854 1101 40-41 -0.1839624289259163 1101 42-43 0.36721541290210524 1101 44-45 0.3048616875059622 1101 46-47 -0.23088181844269684 1101 48-49 -0.17558583340919398 1101 50-51 -0.18365476044277074 1101 52-53 0.17033975649142974 1101 54-55 0.1333247563091291 1101 56-57 -0.5085888954601643 1101 58-59 -0.660066655118996 1101 60-61 -0.5289675202958719 1101 62-63 -0.1031546221346602 1101 64-65 -0.31376237526355766 1101 66-67 -0.1221647004176667 1101 68-69 -0.1171525231700059 1101 70-71 -0.06686813718185292 1101 72-73 0.12137219858611203 1101 74-75 0.18243558687347772 1101 76-77 0.1868436038511021 1101 78-79 0.11753931160078324 1101 80-81 -0.19011007901053745 1101 82-83 0.07884190634014843 1101 84-85 0.03749652657649705 1101 86-87 0.36621354282543095 1101 88-89 0.7933236074729315 1101 90-91 0.8927964488324527 1101 92-93 0.8920776190503616 1101 94-95 0.8248409878643983 1101 96-97 0.8791595648055335 1101 98-99 0.9042129087675406 1101 100-101 1.5212535957146756 1102 1 0.2014496699226349 1102 2 0.2541330316320085 1102 3 0.34900122545253964 1102 4 0.19333727347485308 1102 5 0.2300131739442577 1102 6 0.24356801623783753 1102 7 0.25223107583713755 1102 8 0.20803262968853176 1102 9 0.3348742510643845 1102 10-11 0.36035681602326264 1102 12-13 0.47743531771434533 1102 14-15 0.4021911898188222 1102 16-17 0.5036472312607998 1102 18-19 0.37767447396072384 1102 20-21 0.2617672453506046 1102 22-23 0.5556127393232302 1102 24-25 0.3162644261149907 1102 26-27 0.7822156814262797 1102 28-29 0.6062353893192096 1102 30-31 0.5559879624167507 1102 32-33 0.576397952600928 1102 34-35 0.24088145168622077 1102 36-37 0.3193892100044309 1102 38-39 0.5739802651586388 1102 40-41 1.101580203007181 1102 42-43 0.9877575642046139 1102 44-45 0.4486423277059046 1102 46-47 0.0779713969759257 1102 48-49 -0.10666340718598377 1102 50-51 0.293995917246356 1102 52-53 0.5583768611727749 1102 54-55 0.06661323078764525 1102 56-57 -0.3544987291619748 1102 58-59 -0.19911789033960758 1102 60-61 0.014217137975784055 1102 62-63 0.1366800409432969 1102 64-65 -0.3159492444359131 1102 66-67 -0.33789573954099694 1102 68-69 0.20539866398754825 1102 70-71 0.3206402738534244 1102 72-73 0.5374781295675604 1102 74-75 0.7514301076847794 1102 76-77 0.5368868606039605 1102 78-79 0.5143479245009104 1102 80-81 0.5705599200282023 1102 82-83 0.5877335722057637 1102 84-85 0.5930333909425443 1102 86-87 0.5538517241192906 1102 88-89 0.7126017003307616 1102 90-91 1.3556677359611662 1102 92-93 1.4153161079477954 1102 94-95 1.232656041214394 1102 96-97 1.4534410220774738 1102 98-99 1.8160652590511113 1102 100-101 1.5026772235143504 1103 1 0.23941693224616856 1103 2 0.09757377080934049 1103 3 0.2205832338011149 1103 4 0.1210611138200477 1103 5 -0.09816092670768484 1103 6 0.19012305587012435 1103 7 0.247259451616884 1103 8 0.048471633487601196 1103 9 -0.08888636053919896 1103 10-11 0.06258701160508195 1103 12-13 0.27341111622285297 1103 14-15 0.4740100538495824 1103 16-17 0.2360589380287763 1103 18-19 0.17560843578240792 1103 20-21 0.05081325632570355 1103 22-23 0.07294307092119112 1103 24-25 0.3938170737393989 1103 26-27 0.21624785627736998 1103 28-29 0.2808754155843971 1103 30-31 0.5163204984141245 1103 32-33 0.4387988718823834 1103 34-35 0.4491385690822156 1103 36-37 -0.1916796892029211 1103 38-39 0.20793270641373596 1103 40-41 0.3801787678024411 1103 42-43 0.24904924845957055 1103 44-45 0.30316369161941736 1103 46-47 0.5856867949403721 1103 48-49 0.4572524504191833 1103 50-51 0.5892681840255847 1103 52-53 0.715240985744785 1103 54-55 0.4633816750444879 1103 56-57 0.5985522534373331 1103 58-59 0.7714275812611646 1103 60-61 0.4963708831096838 1103 62-63 0.5116155505347848 1103 64-65 0.5343626546307334 1103 66-67 1.0182906494651505 1103 68-69 1.020803663753039 1103 70-71 1.3227860456277263 1103 72-73 1.3050546972707657 1103 74-75 1.4524408482691804 1103 76-77 1.4966800222755516 1103 78-79 1.2959364991456255 1103 80-81 0.9439359342864435 1103 82-83 1.3423182539749128 1103 84-85 1.4948672636502032 1103 86-87 1.0935895412948433 1103 88-89 1.1464110901342437 1103 90-91 1.009049378473243 1103 92-93 1.2754540001667358 1103 94-95 1.736736525244222 1103 96-97 0.763229493537537 1103 98-99 0.017990590883577795 1103 100-101 1.3405366109727268 1104 1 0.040772473381071705 1104 2 -0.00935535169408297 1104 3 -0.03949319564585352 1104 4 0.11276923985653298 1104 5 0.074093741977876 1104 6 -0.03346912813737646 1104 7 0.31763222542898006 1104 8 0.10925467480276296 1104 9 0.15871620633656391 1104 10-11 0.12996750046513483 1104 12-13 -0.049106729331974464 1104 14-15 -0.034786542876929616 1104 16-17 0.16759690039087616 1104 18-19 0.39328815313766086 1104 20-21 0.34304773610073624 1104 22-23 -0.031137973133901653 1104 24-25 0.17563263362114867 1104 26-27 0.09951269152143283 1104 28-29 0.36866112471987833 1104 30-31 0.265220923823307 1104 32-33 0.289905656798382 1104 34-35 0.6859256481794844 1104 36-37 0.4858385168681707 1104 38-39 0.14790674924307012 1104 40-41 0.4481000310514176 1104 42-43 0.47563371741912164 1104 44-45 0.4067039612855794 1104 46-47 0.37903887511919265 1104 48-49 0.009491256389338787 1104 50-51 0.037245687811008565 1104 52-53 0.30537005056561384 1104 54-55 0.6081289531467249 1104 56-57 0.8325273057121905 1104 58-59 0.6888693578852809 1104 60-61 -0.5481168232858735 1104 62-63 -0.3088834039680606 1104 64-65 0.27572829022089707 1104 66-67 -0.30631819039353303 1104 68-69 -0.7072589218695775 1104 70-71 -0.4118018842879074 1104 72-73 -0.8652171319887358 1104 74-75 -1.0174560440806637 1104 76-77 -0.6416500664114473 1104 78-79 -0.4928370245402931 1104 80-81 -0.3396101036398669 1104 82-83 0.271837325285027 1104 84-85 0.23035648421405241 1104 86-87 -0.19803206083941305 1104 88-89 0.10567275283522903 1104 90-91 0.05094136780196479 1104 92-93 -0.2848964216372849 1104 94-95 0.14945553887173801 1104 96-97 0.07716705212143182 1104 98-99 0.5275442717545857 1104 100-101 0.6286251537660057 1105 1 -0.025451431020364623 1105 2 0.14780144803930995 1105 3 0.16839082920596127 1105 4 0.13647844502685302 1105 5 0.1879264921028465 1105 6 0.113475574188314 1105 7 -0.1070386784249564 1105 8 0.1454135931297884 1105 9 -0.019935987240998543 1105 10-11 0.08803600959412705 1105 12-13 -0.07854089820055066 1105 14-15 -0.07819390796535686 1105 16-17 0.021961441322581265 1105 18-19 -0.22750739067438275 1105 20-21 -0.022933363187512157 1105 22-23 0.14538372459754356 1105 24-25 0.08018880015688978 1105 26-27 0.11969884210821391 1105 28-29 -0.032752857998112006 1105 30-31 -0.34895699043316597 1105 32-33 0.30869924864774845 1105 34-35 0.3453200636618803 1105 36-37 0.554442863555785 1105 38-39 0.22318823654340747 1105 40-41 0.1064113452936013 1105 42-43 0.36221754469422507 1105 44-45 0.4159043707216554 1105 46-47 0.40233150288943165 1105 48-49 0.1358808395738933 1105 50-51 0.2120058582506772 1105 52-53 0.61298270499681 1105 54-55 0.5541405220962332 1105 56-57 0.6423656736809633 1105 58-59 0.6344167405889891 1105 60-61 0.20281750031144696 1105 62-63 0.3775171292510109 1105 64-65 0.14358993891522687 1105 66-67 0.1773757799461606 1105 68-69 -0.19954628886236137 1105 70-71 0.17869062922110146 1105 72-73 0.36123434269525845 1105 74-75 0.5520409684642651 1105 76-77 -0.10889518055365599 1105 78-79 0.4251924453352096 1105 80-81 -0.020987903635106164 1105 82-83 -0.43572533985815554 1105 84-85 -0.050321465749050276 1105 86-87 0.037953085200722825 1105 88-89 0.20970998295258525 1105 90-91 0.1487299369181052 1105 92-93 0.7772569264547897 1105 94-95 0.4840147999824538 1105 96-97 1.2638651406262724 1105 98-99 1.432790766553321 1105 100-101 1.1453120541921038 1106 1 -0.19876099137064074 1106 2 -0.049860373753595866 1106 3 -0.07786266903362815 1106 4 -0.0036768316726139005 1106 5 -0.036931321384557236 1106 6 0.20876310691378563 1106 7 0.1617841075279216 1106 8 0.12230264251139289 1106 9 0.14472953591508997 1106 10-11 -0.053936094766250164 1106 12-13 0.2515010806745934 1106 14-15 0.14369604418774884 1106 16-17 -0.12533701493813965 1106 18-19 0.24575928802924096 1106 20-21 0.36080095115752897 1106 22-23 0.16185930462823706 1106 24-25 0.30598323908439795 1106 26-27 0.3532909699406588 1106 28-29 0.45483403375958176 1106 30-31 0.2599172534957006 1106 32-33 0.4589542815989063 1106 34-35 0.6968028658646475 1106 36-37 0.8370893382330422 1106 38-39 0.809211076662578 1106 40-41 0.4554453797794764 1106 42-43 0.7968343181888642 1106 44-45 0.8416401295011084 1106 46-47 1.005247454861987 1106 48-49 0.8644851548217005 1106 50-51 0.9601664613020446 1106 52-53 1.3287789494673348 1106 54-55 2.0090019466696774 1106 56-57 1.7761112726698585 1106 58-59 1.8830400140384853 1106 60-61 1.9190989844678086 1106 62-63 1.802575809399066 1106 64-65 1.5736138623602045 1106 66-67 1.7629698035987715 1106 68-69 1.2324411626136715 1106 70-71 1.5808527669840338 1106 72-73 1.480072927149532 1106 74-75 1.407823219976411 1106 76-77 1.2376562322837579 1106 78-79 1.2182772155798602 1106 80-81 0.5834514752580944 1106 82-83 0.5509136418155833 1106 84-85 0.7490601357676034 1106 86-87 1.6569383700251343 1106 88-89 1.7281265217266224 1106 90-91 2.461799811432865 1106 92-93 2.084142816136108 1106 94-95 2.5230709523702863 1106 96-97 2.0088998973293535 1106 98-99 2.2490429106356515 1106 100-101 2.1447591066431286 1107 1 0.07443443878341682 1107 2 -0.02060643572347942 1107 3 -0.04086249593340341 1107 4 -0.1760846558027822 1107 5 -0.15156694541087035 1107 6 -0.016786486300688352 1107 7 -0.05212449555326515 1107 8 -0.09632294170187095 1107 9 -0.35146225913424445 1107 10-11 0.043108218891354966 1107 12-13 0.06323294842601968 1107 14-15 -0.23130395581225116 1107 16-17 -0.1325423118050253 1107 18-19 -0.07155506197941719 1107 20-21 -0.24974518005243596 1107 22-23 -0.47168751898262684 1107 24-25 -0.13492467159588273 1107 26-27 -0.13229843632556282 1107 28-29 -0.4428204581912496 1107 30-31 -0.6505899301636333 1107 32-33 -0.1336759763204256 1107 34-35 -0.3861337794529973 1107 36-37 -0.3571940268353302 1107 38-39 -0.3813506335676493 1107 40-41 -0.09090220547238204 1107 42-43 0.15770501232076128 1107 44-45 -0.05075644311443739 1107 46-47 -0.013014618878706585 1107 48-49 -0.07103501135451751 1107 50-51 -0.513543942021478 1107 52-53 -0.19322641663964646 1107 54-55 -0.6417683493317057 1107 56-57 -0.15092698247987357 1107 58-59 -0.07831536072350787 1107 60-61 0.0860974265657859 1107 62-63 0.4088809850957915 1107 64-65 0.3591643210758022 1107 66-67 -0.00868936205814208 1107 68-69 -0.15026179740018009 1107 70-71 0.20059484764873758 1107 72-73 0.20105700192883447 1107 74-75 -0.32283282987645734 1107 76-77 -0.15748830640414369 1107 78-79 -0.2082850139510377 1107 80-81 -0.5305133853962225 1107 82-83 -0.5228703291035792 1107 84-85 0.045580821244495695 1107 86-87 0.2749927234957923 1107 88-89 0.5570169492841792 1107 90-91 0.4886952588969464 1107 92-93 0.2620249348828665 1107 94-95 0.33649054731575845 1107 96-97 0.0911225042187418 1107 98-99 0.4953428933256312 1107 100-101 0.2721392347372955 1108 1 0.13722799481695702 1108 2 -0.03880142277408538 1108 3 0.16839082920596127 1108 4 0.16997126799336115 1108 5 -0.022599823686626053 1108 6 -0.015711028682495964 1108 7 0.05085605841715335 1108 8 0.20761455006758922 1108 9 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2316 88-89 -2.291375618752209 2316 90-91 -3.2031557934505983 2316 92-93 -2.979417513656184 2316 94-95 -2.5487341847789153 2316 96-97 -2.3594210159416136 2316 98-99 -2.6753070180150544 2316 100-101 -2.1990850347870285 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 7.0 5 35.0 6 60.0 7 111.0 8 133.0 9 188.0 10 389.0 11 521.0 12 722.0 13 933.0 14 1148.0 15 1543.0 16 1917.0 17 2476.0 18 2528.0 19 1849.0 20 1834.0 21 2085.0 22 2264.0 23 2337.0 24 2666.0 25 3028.0 26 3263.0 27 3746.0 28 4719.0 29 5492.0 30 6639.0 31 7717.0 32 9587.0 33 12053.0 34 16831.0 35 23284.0 36 31951.0 37 40204.0 38 19285.0 39 685.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.29395334014453 21.116443427971543 16.508571803499958 15.081031428383964 2 14.204627671740583 28.416723847399794 32.533736632544944 24.844911848314684 3 28.475071534264096 25.6645801533844 33.042528462001655 12.817819850349851 4 11.523901546447092 19.14550979540967 42.30674079156806 27.023847866575178 5 9.149850863312375 42.76231953060453 35.16638426386224 12.921445342220853 6 22.91523714833849 39.43976884980372 15.265155228186133 22.379838773671658 7 19.59828784547665 37.3247818963465 23.989768149631477 19.087162108545368 8 22.058226323675626 25.622103037347188 21.059780706053687 31.2598899329235 9 17.684016934832634 25.640307515648846 28.875570056900663 27.80010549261785 10-11 24.582347257425326 24.70744469806239 26.464176854172795 24.24603119033949 12-13 31.69983149188034 24.738719058221655 25.60693263876247 17.95451681113554 14-15 23.250728182531088 28.975129765861308 32.22581127002502 15.548330781582584 16-17 16.478545821196505 31.526813055493314 31.93923183210098 20.0554092912092 18-19 16.02366615319983 35.98142183634412 29.743966764692154 18.2509452457639 20-21 20.932115967194598 27.957877637898925 26.29823603273072 24.81177036217576 22-23 17.561486792417604 28.31216479253896 30.810379353320915 23.315969061722516 24-25 24.831433139229574 29.074020048312 27.28717631545167 18.807370497006755 26-27 21.51956047854439 24.597284265262587 36.7613766319848 17.121778624208222 28-29 16.219256603791198 35.3771361088161 30.232037081121955 18.171570206270744 30-31 18.840701479230557 28.577763463145267 28.804852660421133 23.776682397203043 32-33 19.608557038364772 29.453912329099623 32.05855307072206 18.87897756181354 34-35 23.619336179589588 26.83059484265836 29.716689348014352 19.8333796297377 36-37 20.118169148561172 26.03993286162164 35.70076266022985 18.14113532958734 38-39 19.234701499505142 29.50528468189203 33.097187727586785 18.16282609101604 40-41 18.231533543865055 31.23525169277307 31.362457666085486 19.170757097276393 42-43 17.087180588361605 31.433979703298448 26.906433512897838 24.572406195442113 44-45 20.25286320786644 26.104380177055592 29.072033571943713 24.57072304313425 46-47 24.15558045091724 30.4002707370583 29.629603696961208 15.81454511506325 48-49 21.188250533907503 27.993324697452415 31.085903674917436 19.73252109372265 50-51 14.059123096097203 30.34131381546088 36.366818994449915 19.232744093992 52-53 18.85797239454239 33.437658997446704 27.11440347658857 20.589965131422332 54-55 16.114930939677826 27.342426236854266 30.74082891057867 25.801813912889237 56-57 21.976306171318143 26.29800264198326 28.455700102225144 23.269991084473446 58-59 26.037842417396217 26.070750896814417 28.3666424715435 19.52476421424587 60-61 19.796619030614508 29.448462739551463 33.45633284086851 17.29858538896552 62-63 16.863105712992052 29.79867526175017 32.59028805354967 20.747930971708108 64-65 16.454357892378358 34.800590014563646 28.766010680010456 19.97904141304754 66-67 19.476536571020457 28.689427569820797 25.86532043113146 25.968715428027277 68-69 16.626133149104216 28.938830954225914 29.95994883907649 24.475087057593385 70-71 23.203474721448142 29.073018629249464 26.175472499568226 21.548034149734168 72-73 20.11524835109437 24.911428211339988 34.04097407962359 20.932349357942055 74-75 16.18308103793533 34.58990911764294 27.753427343126408 21.47358250129532 76-77 18.63879408817284 27.704389954472298 27.805303078083867 25.851512879270995 78-79 19.07939419825093 28.25417719864537 30.497576186175042 22.16885241692865 80-81 22.167504930391047 27.342956834280514 27.86948758941804 22.6200506459104 82-83 18.802658787395032 26.487515928918516 32.797001395676666 21.912823888009783 84-85 18.19350893653172 28.964258540934402 30.253275639140565 22.588956883393315 86-87 17.965255213236375 30.416054455457108 28.695867486737697 22.92282284456882 88-89 17.460161206777343 29.62051483978934 25.901836838490873 27.017487114942444 90-91 19.50665292353823 25.520052473763116 28.002014617691156 26.971279985007495 92-93 21.90488860259624 29.5811102864638 28.52641749870468 19.987583612235277 94-95 19.685338922328988 27.334109755613696 29.42461507581787 23.555936246239447 96-97 14.312220806318354 28.994132556608925 32.74658899422591 23.947057642846804 98-99 18.37998814375003 30.594492911923 26.002296564954978 25.02322237937199 100-101 17.120691586691063 26.91848089921207 28.62107434930356 27.339753164793308 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4322.0 1 2664.0 2 807.5 3 549.0 4 431.5 5 406.5 6 598.0 7 1090.5 8 1425.0 9 1317.0 10 932.5 11 570.5 12 477.0 13 448.0 14 425.5 15 405.0 16 364.5 17 340.5 18 343.0 19 362.0 20 397.0 21 415.0 22 455.5 23 522.0 24 602.0 25 720.5 26 964.5 27 1285.5 28 1392.5 29 1513.5 30 1813.5 31 2095.5 32 2541.5 33 3209.5 34 3799.5 35 4218.5 36 4765.0 37 5127.5 38 4876.5 39 4339.5 40 3843.5 41 3490.0 42 3217.0 43 3128.5 44 3181.0 45 3580.5 46 5901.5 47 17412.5 48 37785.0 49 37219.5 50 18710.5 51 8129.5 52 3040.0 53 997.0 54 492.0 55 342.5 56 312.0 57 296.5 58 300.5 59 297.0 60 289.5 61 279.5 62 215.0 63 142.0 64 97.0 65 62.0 66 44.5 67 42.0 68 32.0 69 28.0 70 24.0 71 18.0 72 16.5 73 16.5 74 15.0 75 13.5 76 14.5 77 13.5 78 7.5 79 4.0 80 1.5 81 1.5 82 1.5 83 1.5 84 1.5 85 0.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.400965304131483 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 4.667814949144156E-4 16-17 0.0042010334542297405 18-19 0.0014003444847432468 20-21 0.0 22-23 0.0 24-25 2.333907474572078E-4 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.015637180079632926 36-37 0.022638902503349156 38-39 0.013536663352518052 40-41 0.006301550181344611 42-43 0.005134596444058572 44-45 0.0030340797169437017 46-47 0.0014003444847432468 48-49 0.004901205696601364 50-51 9.335629898288312E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.001166953737286039 60-61 0.0021005167271148702 62-63 9.335629898288312E-4 64-65 4.667814949144156E-4 66-67 0.0028006889694864937 68-69 0.0032674704644009094 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.08752153029645293 78-79 0.0021005167271148702 80-81 2.333907474572078E-4 82-83 0.0 84-85 0.0 86-87 0.0870547488015385 88-89 0.42220386215008887 90-91 0.3701577254671316 92-93 0.0 94-95 0.001166953737286039 96-97 0.0 98-99 0.0 100-101 4.667814949144156E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 214233.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.897340745823474 #Duplication Level Percentage of deduplicated Percentage of total 1 73.52855770734213 15.365513249592732 2 10.76637852085148 4.499773610974966 3 4.431637963769573 2.778283457730602 4 2.5754428287431033 2.152796254545285 5 1.6238915320869352 1.6967507340139008 6 1.0833389175545578 1.3583341502009494 7 0.8197636757577789 1.1991616604351336 8 0.5807590073488351 0.9709055094219845 9 0.5718242533896223 1.0754645642828136 >10 3.5850700261341553 13.904953952005528 >50 0.21666778351091157 3.1652453170146524 >100 0.12955393240858631 6.038752199707795 >500 0.026804261877638545 4.0689342911689605 >1k 0.05360852375527709 28.94231981067343 >5k 0.004467376979606424 6.440651066829107 >10k+ 0.002233688489803212 6.342160171402165 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 13587 6.342160171402165 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 8294 3.8714857188201632 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5504 2.5691653480089434 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4091 1.9096030956948742 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4058 1.8941993063626987 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4014 1.8736609205864643 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3987 1.861057820223775 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3779 1.7639672692815764 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3665 1.7107541788613332 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3649 1.7032856749427026 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3482 1.6253331652919951 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3273 1.5277758328548823 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3105 1.4493565417092604 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2814 1.3135231266891656 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2688 1.2547086583299492 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2229 1.0404559521642325 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2211 1.032053885255773 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2132 0.995178147157534 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2004 0.935430115808489 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1562 0.7291126950563172 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1508 0.7039064943309388 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1457 0.6801006380903035 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1380 0.6441584629818935 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1366 0.6376235220530917 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1321 0.616618354781943 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1152 0.5377322821414068 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1077 0.5027236700228257 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 999 0.4663147134195012 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 928 0.43317322728057767 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 881 0.41123449701960013 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 851 0.3972310521721677 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 845 0.39443036320268116 No Hit CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT 703 0.32814739092483414 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 693 0.32347957597569 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 575 0.268399359575789 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 571 0.2665322335961313 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 570 0.2660654521012169 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 551 0.25719660369784303 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 550 0.2567298222029286 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 453 0.21145201719623027 No Hit ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT 414 0.19324753889456808 RNA PCR Primer, Index 33 (95% over 22bp) CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 400 0.18671259796576622 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 398 0.18577903497593742 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 390 0.18204478301662208 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 368 0.17177559012850493 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 358 0.1671077751793608 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 343 0.16010605275564457 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 340 0.1587057082709013 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 339 0.1582389267759869 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 334 0.15590501930141482 No Hit TACCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 312 0.14563582641329767 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 312 0.14563582641329767 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 307 0.1433019189387256 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 298 0.13910088548449584 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 284 0.13256594455569404 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 283 0.13209916306077962 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 279 0.13023203708112197 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 279 0.13023203708112197 No Hit TGATACCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCA 278 0.12976525558620755 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 263 0.1227635331624913 No Hit TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA 262 0.12229675166757689 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 250 0.11669537372860389 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 245 0.11436146625403182 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 234 0.10922686980997325 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 225 0.10502583635574352 No Hit ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT 221 0.10315871037608584 RNA PCR Primer, Index 33 (95% over 24bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.333907474572078E-4 0.0 18-19 0.0 0.0 0.0 4.667814949144156E-4 0.0 20-21 0.0 0.0 0.0 0.0018671259796576624 0.0 22-23 0.0 0.0 0.0 0.006768331676259027 0.0 24-25 0.0 0.0 0.0 0.014236835594889675 0.0 26-27 0.0 0.0 0.0 0.031040969411808637 0.0 28-29 0.0 0.0 0.0 0.09008882851848221 0.0 30-31 0.0 0.0 0.0 0.2070175929945433 0.0 32-33 0.0 0.0 0.0 0.4128682322518006 0.0 34-35 0.0 0.0 0.0 0.6803340288377607 0.0 36-37 0.0 0.0 0.0 1.0147829699439397 0.0 38-39 0.0 0.0 0.0 1.438853958073686 0.0 40-41 0.0 0.0 0.0 1.8342178842661963 0.0 42-43 0.0 0.0 0.0 2.239851003346823 0.0 44-45 0.0 0.0 0.0 2.7138676114324127 0.0 46-47 0.0 0.0 0.0 3.2611689142195646 0.0 48-49 0.0 0.0 0.0 3.7004103009340295 0.0 50-51 0.0 0.0 0.0 4.1557556492230425 0.0 52-53 0.0 0.0 0.0 4.586594969029048 0.0 54-55 0.0 0.0 0.0 5.085817777840015 0.0 56-57 0.0 0.0 0.0 5.79089122590824 0.0 58-59 0.0 0.0 0.0 6.34846172158351 0.0 60-61 0.0 0.0 0.0 6.942441173862104 0.0 62-63 0.0 0.0 0.0 7.552524587715245 0.0 64-65 0.0 0.0 0.0 8.163774955305673 0.0 66-67 0.0 0.0 0.0 8.915759943612795 0.0 68-69 0.0 0.0 0.0 9.748264739792656 0.0 70-71 0.0 0.0 0.0 10.622546479767356 0.0 72-73 0.0 0.0 0.0 11.351192393328759 0.0 74-75 0.0 0.0 0.0 12.328166062184632 0.0 76-77 0.0 0.0 0.0 13.092053978612071 0.0 78-79 0.0 0.0 0.0 14.327857986397987 0.0 80-81 0.0 0.0 0.0 15.4987793663908 0.0 82-83 0.0 0.0 0.0 16.441211204623002 0.0 84-85 0.0 0.0 0.0 17.54141518813628 0.0 86-87 0.0 0.0 0.0 18.572768901149683 0.0 88-89 0.0 0.0 0.0 19.728053101062862 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTTCGC 25 0.004956446 57.171368 1 CTCGTCC 25 0.005030425 56.957844 9 ATGGAAC 25 0.005030425 56.957844 3 CCTCGTC 25 0.005030425 56.957844 8 GTACCCA 60 2.2844688E-8 55.583275 1 AGGCTGC 70 7.92661E-8 47.46487 8 GTAAGCG 125 0.0 45.566277 94-95 TACCCAT 70 4.3156433E-6 40.684174 2 ACCCATG 75 6.9310663E-6 37.971897 3 TCCGTTT 25 0.0016205641 37.971893 52-53 GAGGCTG 100 1.3001554E-6 33.225407 7 AGGAGGC 115 1.0364238E-7 33.01904 5 GCTTTGC 395 0.0 32.56597 1 CGCAGAA 220 0.0 32.36241 9 CTTTGCG 400 0.0 32.038788 2 TCTCTCC 30 0.0038594524 31.814049 86-87 GGTAGGT 60 0.0038862762 31.761871 1 GATTGGC 30 0.003962536 31.643248 14-15 TCCCCCG 30 0.003962536 31.643248 68-69 GGCTGCT 105 1.903094E-6 31.643248 9 >>END_MODULE